BR112012008516A2 - método para purificar partículas de adenovírus a partir de uma suspensão de célula - Google Patents

método para purificar partículas de adenovírus a partir de uma suspensão de célula

Info

Publication number
BR112012008516A2
BR112012008516A2 BR112012008516A BR112012008516A BR112012008516A2 BR 112012008516 A2 BR112012008516 A2 BR 112012008516A2 BR 112012008516 A BR112012008516 A BR 112012008516A BR 112012008516 A BR112012008516 A BR 112012008516A BR 112012008516 A2 BR112012008516 A2 BR 112012008516A2
Authority
BR
Brazil
Prior art keywords
cell suspension
adenovirus particles
purifying adenovirus
purifying
particles
Prior art date
Application number
BR112012008516A
Other languages
English (en)
Other versions
BR112012008516B1 (pt
Inventor
Marcel Leo De Vocht
Marloes Veenstra
Original Assignee
Crucell Holland Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv
Publication of BR112012008516A2 publication Critical patent/BR112012008516A2/pt
Publication of BR112012008516B1 publication Critical patent/BR112012008516B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/861—Adenoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

método para purificar partículas de adenovírus a partir de uma suspensão de célula. a invenção fornece um método para a purificação de adenovírus em larga escala a partir de suspensões de alta densidade celular, usando precipitação do dna da célula hospedeira seguida por uma etapa de clarificação.
BR112012008516-6A 2009-10-15 2010-10-14 Método para purificar partículas de adenovírus a partir de uma suspensão de célula BR112012008516B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27901409P 2009-10-15 2009-10-15
EP09173090 2009-10-15
US61/279,014 2009-10-15
EP09173090.3 2009-10-15
PCT/EP2010/065430 WO2011045378A1 (en) 2009-10-15 2010-10-14 Method for the purification of adenovirus particles

Publications (2)

Publication Number Publication Date
BR112012008516A2 true BR112012008516A2 (pt) 2015-09-15
BR112012008516B1 BR112012008516B1 (pt) 2021-10-19

Family

ID=41618043

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012008516-6A BR112012008516B1 (pt) 2009-10-15 2010-10-14 Método para purificar partículas de adenovírus a partir de uma suspensão de célula

Country Status (16)

Country Link
US (1) US8460920B2 (pt)
EP (1) EP2488636B1 (pt)
JP (1) JP5393896B2 (pt)
KR (1) KR101749779B1 (pt)
CN (1) CN102791852B (pt)
AU (1) AU2010305765B2 (pt)
BR (1) BR112012008516B1 (pt)
CA (1) CA2776461C (pt)
DK (1) DK2488636T3 (pt)
EA (1) EA028623B1 (pt)
ES (1) ES2472429T3 (pt)
HK (1) HK1174948A1 (pt)
MX (1) MX2012004222A (pt)
PL (1) PL2488636T3 (pt)
WO (1) WO2011045378A1 (pt)
ZA (1) ZA201202523B (pt)

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US8932607B2 (en) 2012-03-12 2015-01-13 Crucell Holland B.V. Batches of recombinant adenovirus with altered terminal ends
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KR101906319B1 (ko) 2017-12-04 2018-10-10 주식회사 삼양바이오팜 재조합 3형 아데노바이러스 섬유 폴리펩타이드의 정제방법
GB202019454D0 (en) * 2020-12-10 2021-01-27 Chancellor Masters And Scholars Of The Univ Of Oxford Method for purifying virus
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Also Published As

Publication number Publication date
AU2010305765B2 (en) 2015-07-02
EP2488636B1 (en) 2014-03-12
BR112012008516B1 (pt) 2021-10-19
ZA201202523B (en) 2012-12-27
EA028623B1 (ru) 2017-12-29
EA201290200A1 (ru) 2012-08-30
KR101749779B1 (ko) 2017-06-21
CN102791852B (zh) 2014-05-07
US8460920B2 (en) 2013-06-11
MX2012004222A (es) 2012-06-08
AU2010305765A1 (en) 2012-04-12
CN102791852A (zh) 2012-11-21
US20120202267A1 (en) 2012-08-09
JP2013507140A (ja) 2013-03-04
CA2776461C (en) 2020-08-25
EP2488636A1 (en) 2012-08-22
PL2488636T3 (pl) 2014-07-31
JP5393896B2 (ja) 2014-01-22
CA2776461A1 (en) 2011-04-21
HK1174948A1 (en) 2013-06-21
ES2472429T3 (es) 2014-07-01
DK2488636T3 (da) 2014-06-23
KR20120083896A (ko) 2012-07-26
WO2011045378A1 (en) 2011-04-21

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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Owner name: JANSSEN VACCINES AND PREVENTION B.V (NL)

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
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Owner name: JANSSEN VACCINES AND PREVENTION B.V. (NL)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 14/10/2010, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.