HK1174949A1 - Process for adenovirus purification from high cell density cultures - Google Patents

Process for adenovirus purification from high cell density cultures

Info

Publication number
HK1174949A1
HK1174949A1 HK13102162.3A HK13102162A HK1174949A1 HK 1174949 A1 HK1174949 A1 HK 1174949A1 HK 13102162 A HK13102162 A HK 13102162A HK 1174949 A1 HK1174949 A1 HK 1174949A1
Authority
HK
Hong Kong
Prior art keywords
cell density
high cell
density cultures
adenovirus purification
adenovirus
Prior art date
Application number
HK13102162.3A
Other languages
English (en)
Inventor
Vocht Marcel Leo De
Marloes Veenstra
Original Assignee
Crucell Holland Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv
Publication of HK1174949A1 publication Critical patent/HK1174949A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
HK13102162.3A 2009-10-15 2013-02-20 Process for adenovirus purification from high cell density cultures HK1174949A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27906009P 2009-10-15 2009-10-15
EP09173119 2009-10-15
PCT/EP2010/065436 WO2011045381A1 (en) 2009-10-15 2010-10-14 Process for adenovirus purification from high cell density cultures

Publications (1)

Publication Number Publication Date
HK1174949A1 true HK1174949A1 (en) 2013-06-21

Family

ID=41563777

Family Applications (1)

Application Number Title Priority Date Filing Date
HK13102162.3A HK1174949A1 (en) 2009-10-15 2013-02-20 Process for adenovirus purification from high cell density cultures

Country Status (16)

Country Link
US (1) US8470585B2 (ru)
EP (1) EP2488635B1 (ru)
JP (1) JP5465331B2 (ru)
KR (1) KR101805938B1 (ru)
CN (1) CN102575233B (ru)
AU (1) AU2010305768B2 (ru)
BR (1) BR112012008507B8 (ru)
CA (1) CA2777116C (ru)
DK (1) DK2488635T3 (ru)
EA (1) EA028875B1 (ru)
ES (1) ES2445713T3 (ru)
HK (1) HK1174949A1 (ru)
MX (1) MX2012004221A (ru)
PL (1) PL2488635T3 (ru)
WO (1) WO2011045381A1 (ru)
ZA (1) ZA201202524B (ru)

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US10744196B2 (en) 2016-07-14 2020-08-18 Janssen Vaccines & Prevention B.V. HPV vaccines
MX2019009316A (es) 2017-02-09 2019-09-19 Janssen Vaccines & Prevention Bv Promotor corto y potente para la expresion de genes heterologos.
US11229692B2 (en) 2017-05-17 2022-01-25 Janssen Vaccines & Prevention B.V. Methods and compositions for inducing protective immunity against RSV infection
SG11202001458SA (en) 2017-09-15 2020-03-30 Janssen Vaccines & Prevention Bv Method for the safe induction of immunity against rsv
US20210047626A1 (en) 2018-04-24 2021-02-18 Merck Sharp & Dohme Corp. Scalable chromatography process for purification of human cytomegalovirus
CA3108544A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna
CN111454914A (zh) * 2019-01-18 2020-07-28 嘉兴安宇生物科技有限公司 一种腺病毒快速纯化方法
KR102125567B1 (ko) * 2019-07-02 2020-06-22 한양대학교 에리카산학협력단 식물 엑소좀의 대량 생산 방법
AU2020400034A1 (en) * 2019-12-10 2022-05-26 Repligen Corporation Methods of preparing viral vectors
JP2024500797A (ja) * 2020-12-21 2024-01-10 ファイザー・インク 過剰な核酸沈殿を阻害するための方法および組成物
JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
CN115073549B (zh) * 2022-07-07 2023-10-20 澳斯康生物(南通)股份有限公司 Hek293细胞裂解液的纯化方法
CN117551624A (zh) * 2024-01-11 2024-02-13 深圳源兴基因技术有限公司 一种基于无血清悬浮扩增的高效回收腺病毒的方法

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Also Published As

Publication number Publication date
MX2012004221A (es) 2012-06-08
BR112012008507B1 (pt) 2021-05-04
DK2488635T3 (en) 2014-02-24
PL2488635T3 (pl) 2014-04-30
EA201270557A1 (ru) 2012-09-28
JP2013507141A (ja) 2013-03-04
EA028875B1 (ru) 2018-01-31
WO2011045381A1 (en) 2011-04-21
CA2777116C (en) 2020-09-01
AU2010305768A1 (en) 2012-04-12
CN102575233A (zh) 2012-07-11
US8470585B2 (en) 2013-06-25
AU2010305768B2 (en) 2015-05-14
CA2777116A1 (en) 2011-04-21
BR112012008507A2 (pt) 2015-09-15
ES2445713T3 (es) 2014-03-04
JP5465331B2 (ja) 2014-04-09
BR112012008507B8 (pt) 2021-05-25
US20120202268A1 (en) 2012-08-09
KR101805938B1 (ko) 2018-01-10
EP2488635A1 (en) 2012-08-22
ZA201202524B (en) 2012-12-27
EP2488635B1 (en) 2013-11-20
KR20120093957A (ko) 2012-08-23
CN102575233B (zh) 2014-07-16

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20221016