EP2376515A1 - Synthesis of purine nucleosides - Google Patents

Synthesis of purine nucleosides

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Publication number
EP2376515A1
EP2376515A1 EP09793688A EP09793688A EP2376515A1 EP 2376515 A1 EP2376515 A1 EP 2376515A1 EP 09793688 A EP09793688 A EP 09793688A EP 09793688 A EP09793688 A EP 09793688A EP 2376515 A1 EP2376515 A1 EP 2376515A1
Authority
EP
European Patent Office
Prior art keywords
alkyl
compound
cycloalkyl
salt
lower alkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09793688A
Other languages
German (de)
English (en)
French (fr)
Inventor
Byoung-Kwon Chun
Jinfa Du
Suguna Rachakonda
Bruce S. Ross
Michael Joseph Sofia
Ganapati Reddy Pamulapati
Wonsuk Chang
Hai-Ren Zhang
Dhanapalan Nagarathnam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gilead Pharmasset LLC
Original Assignee
Pharmasset Inc
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Filing date
Publication date
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Application filed by Pharmasset Inc filed Critical Pharmasset Inc
Priority to EP13182837.8A priority Critical patent/EP2671888A1/en
Publication of EP2376515A1 publication Critical patent/EP2376515A1/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/04—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/167—Purine radicals with ribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C07H19/213—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids containing cyclic phosphate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
    • C07H5/02—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to halogen
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Definitions

  • Hepatitis C virus (HCV) infection is a major health problem that leads to chronic liver disease, such as cirrhosis and hepatocellular carcinoma, in a substantial number of infected individuals, estimated to be 2-15% of the world's population.
  • chronic liver disease such as cirrhosis and hepatocellular carcinoma
  • According to the World Health Organization there are more than 200 million infected individuals worldwide, with at least 3 to 4 million people being infected each year. Once infected, about 20% of people clear the virus, but the rest can harbor HCV the rest of their lives.
  • Ten to twenty percent of chronically infected individuals eventually develop liver- destroying cirrhosis or cancer.
  • the viral disease is transmitted parenterally by contaminated blood and blood products, contaminated needles, or sexually and vertically from infected mothers or carrier mothers to their offspring.
  • Current treatments for HCV infection which are restricted to immunotherapy with recombinant interferon- ⁇ alone or in combination with the nucleoside analog ribavirin, are of limited clinical benefit.
  • Purine phosphoramidates have been shown to be potent inhibitors of the HCV virus (US Patent Application 12/053,015, see also WO 2008/121634). However, preparation of these compounds has been made difficult due to poor yields associated with the coupling of the ribose sugar to the purine base and because of poor C-I' beta-stereoselectivity associated with the ribose to purine base coupling step.
  • nucleoside analogue there are two ways to prepare a nucleoside analogue.
  • the first way follows a linear synthetic sequence in which the target nucleoside is prepared from an appropriate nucleoside. In this approach, usually there is less concern about stereoselective chemistry as most if not all of the stereocenters are set. However, the synthesis can be lengthy if extensive modification of the sugar is required.
  • a common way to avoid this stereochemical problem is to employ an ⁇ -halosugar so that an Sj ⁇ 2 type coupling with a salt of a purine base or a silylated pyrimidine base would generate the desired ⁇ isomer enriched mixture (Kazimierczuk, Z. et al. J. Am. Chem. Soc. 1984, 106, 6379-6382; Chun, B. K. et al J. Org. Chem, 2000, 65, 685-693; Zhong, M. et al. J. Org. Chem. 2006, 71, 1113- 7779).
  • Another possible way to do an Sj ⁇ 2 type coupling is enzymatic glycosylation in which the sugar- 1- ⁇ -O-phosphate is coupled with purine base using either isolated enzymes or whole cells.
  • the phosphate intermediate can be generated enzymatically from another nucleoside containing the desired sugar.
  • This coupled reaction is called transglycosylation.
  • This conversion is highly stereospecific.
  • natural enzymes only work with a limited number of modified sugars.
  • existing enzymes from a range of microorganisms need to be screened for activity or through extensive research there is a possibility that a mutated enzyme can be selected and produced though genetic engineering ( Komatsu, H. et al Tetrahedron Lett. 2003, 44, 2899-2901; Okuyama, K.
  • a final alternative method to couple a sugar with a purine base is through the use of Mitsunobu chemistry.
  • This approach uses a condensing reagent such as N, N- dicyclohexylcarbodiiomide (DCC) and triphenylphosphine.
  • DCC dicyclohexylcarbodiiomide
  • triphenylphosphine triphenylphosphine
  • 2'-deoxy-2'-fluoro-2'-C-methyl purine nucleosides and their corresponding nucleotide phosphoramidates belong to the 2'-deoxy nucleoside category since there is no directing ⁇ -acyloxy group in 2'-position.
  • a close derivative of the purine analogs was first prepared using the linear nucleoside route in a less than 5% overall yield due to the complexity of forming the 2' quaternary center. The lowest yielding step, fluorination, was done late in the sequence. This route was unsuitable for large scale synthesis (Clark, J. L. et al. Bioorg. Med. Chem. Lett. 2006, 16, 1712-1715).
  • R 1 is hydrogen, n-alkyl; branched alkyl; cycloalkyl; or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of Ci_6 alkyl, C2-6 alkenyl, C2-6 alkynyl, Ci_6 alkoxy, F, Cl, Br, I, nitro, cyano,
  • R 1 is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 2 o alkyl, Ci_io alkyl, or Ci_ 6 alkyl, R 1 is - OR' or -N(R r ) 2 );
  • R 2 is hydrogen, C 1-10 alkyl, or R 3a or R 3b and R 2 together are (CH 2 ),, so as to form a cyclic ring that includes the adjoining N and C atoms, where n is 2 to 4;
  • R 3a and R 3b together are(CH 2 ) f so as to form a spiro ring;
  • R 3a is hydrogen and R 3b and R 2 together are (CH 2 ) n so as to form a cyclic ring that includes the adjoining N and C atoms
  • R 3b is hydrogen and R 3a and R 2 together are (CH 2 ) n so as to form a cyclic ring that includes the adjoining N and C atoms, where c is 1 to 6, d is
  • R 3 is independently hydrogen or Ci_ 6 alkyl and R 3" is -OR or -N(R 3' ) 2 );
  • R 3a is H and R 3b is H, CH 3 , CH 2 CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 -indol-3-yl, -CH 2 CH 2 SCH 3 , CH 2 CO 2 H, CH 2 C(O)NH 2 , CH 2 CH 2 COOH, CH 2 CH 2 C(O)NH 2 , CH 2 CH 2 CH 2 CH 2 NH 2 , -
  • R 3a is CH 3 , -CH 2 CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 ,
  • R 3b is H, where R 3' is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 20 alkyl, C 1-10 alkyl, or C 1-6 alkyl, R 3" is -OR' or -N(R 3' ) 2 );
  • R 4 is hydrogen, C 1-10 alkyl, C 1-10 alkyl optionally substituted with a lower alkyl, alkoxy, di(lower alkyl)-amino, or halogen, C 1-10 haloalkyl, C 3 -Io cycloalkyl, cycloalkyl alkyl, cycloheteroalkyl, aminoacyl, aryl, such as phenyl, heteroaryl, such as, pyridinyl, substituted aryl, or substituted heteroaryl;
  • R 5 is H, a lower alkyl, CN, vinyl, O-(lower alkyl), hydroxyl lower alkyl, i.e., -(CH 2 ) P OH, where p is 1 -6, including hydroxyl methyl (CH 2 OH), CH 2 F, N 3 , CH 2 CN, CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , alky
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , F, CN, vinyl, or ethynyl;
  • R 7 is hydrogen, n-alkyl, branched alkyl, cycloalkyl, alkaryl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkenyl Of C 2 -C 6 , lower alkynyl Of C 2 -C 6 , such as C ⁇ CH, halogenated (F,
  • X is H, OH, OMe, halogen, CN, NH 2 , or N 3 ;
  • O) Z is N or CR 10 ;
  • R 5 , R 6 , and X have the meanings as defined above, X' is a leaving group and R 11 is a protecting group.
  • a or “an” entity refers to one or more of that entity; for example, a compound refers to one or more compounds or at least one compound.
  • a compound refers to one or more compounds or at least one compound.
  • the terms “a” (or “an”), “one or more”, and “at least one” can be used interchangeably herein.
  • halo includes chloro, bromo, iodo and fluoro.
  • both R's can be carbon, both R's can be nitrogen, or one R' can be carbon and the other nitrogen.
  • purified refers to the purity of a given compound.
  • a compound is “purified” when the given compound is a major component of the composition, i.e., at least 50% w/w pure.
  • purified embraces at least 50% w/w purity, at least 60% w/w purity, at least 70% purity, at least 80% purity, at least 85% purity, at least 90% purity, at least 92% purity, at least 94% purity, at least 96% purity, at least 97% purity, at least 98% purity, and at least 99% purity.
  • tautomerism and “tautomers” have their accepted plain meanings.
  • P* means that the phosphorus atom is chiral and that it has a corresponding Cahn-Ingold-Prelog designation of "R” or "S” which have their accepted plain meanings. It is contemplated that the phosphoramidate nucleoside I and the cyclic phosphate nucleotide II can exist as a mixture of diastereomers due to the chirality at phosphorus. Applicants contemplate use of the mixture of disastereomers and/or the resolved diastereomers. In some instances, an asterisk does not appear next to the phosphoroamidate or cyclic phosphate phosphorus atom.
  • isotopically-enriched analogs of compounds I and II are also contemplated.
  • isotopically-enriched means that at least one atom of compounds I and II is enriched with a particular isotope, such as, for example, 2 H, 3 H, 13 C, 15 N, 32 P, etc.
  • deuterated analogs means a compound described herein or its salts thereof, whereby a hydrogen atom is enriched with its 2 H-isotope, i.e., deuterium (D).
  • Deuterium substitution can be partial or complete. Partial deuterium substitution means that at least one hydrogen is substituted by at least one deuterium.
  • methyl groups depicted below are shown as being completely deuterated, one will recognize that partial-deuterated variations are also possible, such as, -CDH 2 and -CD 2 H.
  • alkenyl refers to an unsubstituted hydrocarbon chain radical having from 2 to 10 carbon atoms having one or two olefmic double bonds, preferably one olefmic double bond.
  • C 2 -N alkenyl refers to an alkenyl comprising 2 to N carbon atoms, where N is an integer having the following values: 3, 4, 5, 6, 7, 8, 9, or 10.
  • C 2 -io alkenyl refers to an alkenyl comprising 2 to 10 carbon atoms.
  • C 2 _ 4 alkenyl refers to an alkenyl comprising 2 to 4 carbon atoms. Examples include, but are not limited to, vinyl, 1-propenyl, 2- propenyl (allyl) or 2-butenyl (crotyl).
  • halogenated alkenyl refers to an alkenyl comprising at least one of F, Cl, Br, and I.
  • alkyl refers to an unbranched or branched chain, saturated, monovalent hydrocarbon residue containing 1 to 30 carbon atoms.
  • C I _ M alkyl refers to an alkyl comprising 1 to M carbon atoms, where M is an integer having the following values: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30.
  • C 1-4 alkyl refers to an alkyl containing 1 to 4 carbon atoms.
  • lower alkyl denotes a straight or branched chain hydrocarbon residue comprising 1 to 6 carbon atoms, which is also designated by the expression "Ci_6-alkyl.”
  • Ci_2o alkyl refers to an alkyl comprising 1 to 20 carbon atoms.
  • C 1 -H alkyl refers to an alkyl comprising 1 to 10 carbons.
  • alkyl groups include, but are not limited to, methyl, ethyl, propyl, /-propyl, n-butyl, /-butyl, /-butyl, pentyl, isopentyl, neopentyl, hexyl, heptyl, octyl, nonyl, decyl, etc..
  • lower alkyl groups include, but are not limited to, methyl, ethyl, propyl, /-propyl, n-butyl, /-butyl, /-butyl, pentyl, isopentyl, neopentyl, hexyl, etc.
  • the term (ar)alkyl or (heteroaryl)alkyl indicate the alkyl group is optionally substituted by an aryl or a heteroaryl group respectively.
  • halogenated alkyl refers to an unbranched or branched chain alkyl comprising at least one of F, Cl, Br, and I.
  • C I _ M haloalkyl refers to an alkyl comprising 1 to M carbon atoms that comprises at least one of F, Cl, Br, and I, where M is an integer having the following values: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30.
  • Ci- 3 haloalkyl refers to a haloalkyl comprising 1 to 3 carbons and at least one of F, Cl, Br, and I.
  • halogenated lower alkyl refers to a haloalkyl comprising 1 to 6 carbon atoms and at least one of F, Cl, Br, and I.
  • Examples include, but are not limited to, fluoromethyl, chloromethyl, bromomethyl, iodomethyl, difluoromethyl, dichloromethyl, dibromomethyl, diiodomethyl, trifluoromethyl, trichloromethyl, tribromomethyl, triiodomethyl, 1- fluoroethyl, 1-chloroethyl, 1-bromoethyl, 1-iodoethyl, 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 2-iodoethyl, 2,2-difluoroethyl, 2,2-dichloroethyl, 2,2-dibromomethyl, 2-2-diiodomethyl, 3-
  • alkynyl refers to an unbranched or branched hydrocarbon chain radical having from 2 to 10 carbon atoms, preferably 2 to 5 carbon atoms, and having one triple bond.
  • C 2 -N alkynyl refers to an alkynyl comprising 2 to N carbon atoms, where N is an integer having the following values: 3, 4, 5, 6, 7, 8, 9, or 10.
  • C C2-4 alkynyl refers to an alkynyl comprising 2 to 4 carbon atoms.
  • C 2 - 10 alkynyl refers to an alkynyl comprising 2 to 10 carbons. Examples include, but are limited to, ethynyl (i.e., -C ⁇ CH), 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl or 3-butynyl.
  • halogenated alkynyl refers to an unbranched or branched hydrocarbon chain radical having from 2 to 10 carbon atoms, preferably 2 to 5 carbon atoms, and having one triple bond and at least one of F, Cl, Br, and I.
  • alkoxy refers to an -O-alkyl group, an -O-cycloalkyl group, an - O-lower cycloalkyl, wherein alkyl, cycloalkyl, and lower cycloalkyl are as defined above.
  • -O-alkyl groups include, but are not limited to, methoxy, ethoxy, n-propyloxy, z-propyloxy, n-butyloxy, z-butyloxy, t-butyloxy.
  • “Lower alkoxy” as used herein denotes an alkoxy group with a "lower alkyl” group as previously defined.
  • C 1 -H) alkoxy refers to an-O-alkyl wherein alkyl is C 1-10 .
  • -O-cycloalkyl groups include, but are not limited to, -O-c-propyl, -O-c- butyl, -O-c-pentyl, and -O-c-hexyl.
  • halogenated alkoxy refers to an -O-alkyl group in which the alkyl group comprises at least one of F, Cl, Br, and I.
  • halogenated lower alkoxy refers to an -O-(lower alkyl) group in which the lower alkyl group comprises at least one of F, Cl, Br, and I.
  • cycloalkyl refers to an unsubstituted or substituted carbocycle, in which the carbocycle contains 3 to 10 carbon atoms; preferably 3 to 8 carbon atoms; more preferably 3 to 6 carbon atoms (i.e., lower cycloalkyls).
  • Examples of cycloalkyl groups include, but are not limited to, cyclopropyl, 2-methyl-cyclopropyl, cyclobutyl, cyclopentyl, cycloheptyl, or cyclooctyl.
  • C 3 - 7 cycloalkyl refers to a cycloalkyl comprising 3 to 7 carbons in the carbocyclic ring.
  • lower cycloalkyl refers to C 3-6 cycloalkyl rings, which include, but are not limited to, cyclopropyl ( c Pr), 2-methyl-cyclopropyl, etc., cyclobutyl ( c Bu), 2- methyl-cyclobutyl, 3-methyl-cyclobutyl, etc., cyclopentyl ( c Pn), 2-methyl- cyclopentyl, 3-methyl-cyclopentyl, 4-methyl-cyclopentyl, etc., cyclohexyl ( ⁇ x), etc.
  • cycloalkyl alkyl refers to an additionally unsubstituted or substituted alkyl substituted by a lower cycloalkyl.
  • cycloalkyl alkyls include, but are not limited to, any one of methyl, ethyl, propyl, i-propyl, n-butyl, i- butyl, t-butyl or pentyl, isopentyl, neopentyl, hexyl, heptyl, and octyl that is substituted with cyclopropyl, 2-methyl-cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.
  • cycloheteroalkyl refers to an unsubstituted or substituted heterocycle, in which the heterocycle contains 2 to 9 carbon atoms; preferably 2 to 7 carbon atoms; more preferably 2 to 5 carbon atoms.
  • Examples of cycloheteroalkyls include, but are not limited to, aziridin-2-yl, N-Ci_ 3 -alkyl-aziridin-2-yl, azetidinyl, N- Ci_ 3 -alkyl-azetidin-m'-yl, pyrrolidin-m'-yl, N-Ci_ 3 -alkyl-pyrrolidin-m'-yl, piperidin- m'-yl, and N-Ci_ 3 -alkyl-piperidin-m'-yl, where m' is 2, 3, or 4 depending on the cycloheteroalkyl.
  • ⁇ -Ci_ 3 -alkyl-cycloheteroalkyls include, but are not limited to, N-methyl-aziridin-2-yl, N-methyl-azetidin-3-yl, N-methyl- pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl-piperidin-2-yl, N-methyl- piperidin-3-yl, and N-methyl-piperidin-4-yl.
  • R 4 the point of attachment between the cycloheteroalkyl ring carbon and the oxygen occurs at any one of m'.
  • acyl refers to a substituent containing a carbonyl moiety and a non-carbonyl moiety.
  • the carbonyl moiety contains a double-bond between the carbonyl carbon and a heteroatom, where the heteroatom is selected from among O, N and S.
  • the heteroatom is selected from among O, N and S.
  • the heteroatom is N, the N is substituted by a lower alkyl.
  • the non- carbonyl moiety is selected from straight, branched, and cyclic alkyl, which includes, but is not limited to, a straight, branched, or cyclic Ci_2o alkyl, C 1-10 alkyl, or lower alkyl; alkoxyalkyl, including methoxymethyl; aralkyl, including benzyl; aryloxyalkyl, such as phenoxymethyl; or aryl, including phenyl optionally substituted with halogen (F, Cl, Br, I), hydroxyl, Ci to C 4 alkyl, or Ci to C 4 alkoxy, sulfonate esters, such as alkyl or aralkyl sulphonyl, including methanesulfonyl, the mono, di or triphosphate ester, trityl or monomethoxytrityl, substituted benzyl, trialkylsilyl (e.g. dimethyl-t-butylsilyl) or diphenyl
  • lower acyl refers to an acyl group in which the non-carbonyl moiety is lower alkyl.
  • aryl refers to substituted or unsubstituted phenyl (Ph), biphenyl, or naphthyl, preferably the term aryl refers to substituted or unsubstituted phenyl.
  • the aryl group can be substituted with one or more moieties selected from among hydroxyl, F, Cl, Br, I, amino, alkylamino, arylamino, alkoxy, aryloxy, nitro, cyano, sulfonic acid, sulfate, phosphonic acid, phosphate, and phosphonate, either unprotected, or protected as necessary, as known to those skilled in the art, for example, as taught in T. W. Greene and P. G. M. Wuts, "Protective Groups in Organic Synthesis," 3rd ed., John Wiley & Sons, 1999.
  • alkaryl or “alkylaryl” refer to an alkyl group with an aryl substituent, such as benzyl.
  • lower alkaryl or “lower alkylaryl” refer to a lower alkyl group with an aryl substituent, such as benzyl.
  • aralkyl or “arylalkyl” refer to an aryl group with an alkyl substituent.
  • di(lower alkyl)amino-lower alkyl refers to a lower alkyl substituted by an amino group that is itself substituted by two lower alkyl groups. Examples include, but are not limited to, (CH 3 ) 2 NCH 2 , (CHs) 2 NCH 2 CH 2 , (CHs) 2 NCH 2 CH 2 CH 2 , etc.
  • the examples above show lower alkyls substituted at the terminus carbon atom with an N,N-dimethyl-amino substituent. These are intended as examples only and are not intended to limit the meaning of the term "di(lower alkyl)amino-lower alkyl" so as to require the same.
  • the lower alkyl chain can be substituted with an N,N-di(lower alkyl)-amino at any point along the chain, e.g., CH 3 CH(N-(lower alkyl) 2 )CH 2 CH 2 .
  • heterocycle refers to an unsubstituted or substituted heterocycle containing carbon, hydrogen, and at least one of N, O, and S, where the C and N can be trivalent or tetravalent, i.e., sp 2 - or sp 3 -hybridized.
  • heterocycles include, but are not limited to, aziridine, azetidine, pyrrolidine, piperidine, imidazole, oxazole, piperazine, etc.
  • nitrogen heterocycle as used for R 8 and R 9 represents a heterocycle containing a nitrogen where the nitrogen is the point of attachment to the purine.
  • Examples of a nitrogen heterocycle include, but are not limited to, -N(-CH 2 CH 2 -) (aziridin-1-yl), -N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl), -N(-CH 2 CH 2 CH 2 CH 2 -) (pyrrolidin-1-yl), etc.
  • piperazine as related to R 10 for NR' 2 , the corresponding opposite nitrogen atom of the piperazinyl is substituted by a lower alkyl represented by the following structure:
  • the opposite nitrogen of the piperazinyl is substituted by a methyl group.
  • amino acid includes naturally occurring and synthetic ⁇ , ⁇ ⁇ or ⁇ amino acids, and includes but is not limited to, amino acids found in proteins, i.e. glycine, alanine, valine, leucine, isoleucine, methionine, phenylalanine, tryptophan, proline, serine, threonine, cysteine, tyrosine, asparagine, glutamine, aspartate, glutamate, lysine, arginine and histidine.
  • the amino acid is in the L-configuration.
  • the amino acid can be a derivative of alanyl, valinyl, leucinyl, isoleucinyl, prolinyl, phenylalaninyl, tryptophanyl, methioninyl, glycinyl, serinyl, threoninyl, cysteinyl, tyrosinyl, asparaginyl, glutaminyl, aspartoyl, glutaroyl, lysinyl, argininyl, histidinyl, ⁇ -alanyl, ⁇ -valinyl, ⁇ -leucinyl, ⁇ -isoleucinyl, ⁇ -prolinyl, ⁇ -phenylalaninyl, ⁇ -tryptophanyl, ⁇ -methioninyl, ⁇ -glycinyl, ⁇ -serinyl, ⁇ - threoninyl, ⁇ -cysteiny
  • amino acid When the term amino acid is used, it is considered to be a specific and independent disclosure of each of the esters of ⁇ , ⁇ ⁇ or ⁇ glycine, alanine, valine, leucine, isoleucine, methionine, phenylalanine, tryptophan, proline, serine, threonine, cysteine, tyrosine, asparagine, glutamine, aspartate, glutamate, lysine, arginine and histidine in the D and L-conf ⁇ gurations.
  • aminoacyl includes unsubstituted, N -monosubstituted, and N,N- disubstituted derivatives of naturally occurring and synthetic ⁇ , ⁇ ⁇ or ⁇ amino acyls, where the amino acyls are derived from amino acids.
  • the amino-nitrogen can be substituted or unsubstituted. When the amino-nitrogen is substituted, the nitrogen is either mono- or di-substituted, where the substituent bound to the amino-nitrogen is a lower alkyl or an alkaryl.
  • alkylamino or arylamino refer to an amino group that has one or two alkyl or aryl substituents, respectively.
  • protected refers to a group that is added to an oxygen, nitrogen, or phosphorus atom to prevent its further reaction or for other purposes.
  • oxygen and nitrogen protecting groups are known to those skilled in the art of organic synthesis.
  • Non-limiting examples include: C(O)-alkyl, C(O)Ph, C(O)aryl, CH 3 , CH 2 -alkyl, CH 2 -alkenyl, CH 2 Ph, CH 2 -aryl, CH 2 O-alkyl, CH 2 O-aryl, SO 2 -alkyl, SO 2 -aryl, tert- butyldimethylsilyl, tert-butyldiphenylsilyl, and 1, 3-(l, 1,3,3- tetraisopropyldisiloxanylidene).
  • purine or "pyrimidine” base includes, but is not limited to, adenine, N 6 -alkylpurines, N 6 -acylpurines (wherein acyl is C(O)(alkyl, aryl, alkylaryl, or arylalkyl), N 6 -benzylpurine, N 6 -halopurine, N 6 -vinylpurine, N 6 - acetylenic purine, N 6 -acyl purine, N 6 -hydroxyalkyl purine, N 6 -alkylaminopurine, N 6 -thioalkyl purine, N 2 -alkylpurines, N 2 -alkyl-6-thiopurines, thymine, cytosine, 5- fluorocytosine, 5-methylcytosine, 6-azapyrimidine, including 6-azacytosine, 2- and/or 4-mercaptopyrmidine, uracil, 5-halouracil, including 5-fluorouracil,
  • Purine bases include, but are not limited to, guanine, adenine, hypoxanthine, 2,6- diaminopurine, and 6-chloropurine. Functional oxygen and nitrogen groups on the base can be protected as necessary or desired. Suitable protecting groups are well known to those skilled in the art, and include trimethylsilyl, dimethylhexylsilyl, t- butyldimethylsilyl, and t-butyldiphenylsilyl, trityl, alkyl groups, and acyl groups such as acetyl and propionyl, methanesulfonyl, and p-toluenesulfonyl.
  • protecting group refers to a chemical group which exhibits the following characteristics. The group must react selectively in good yield to give a protected substrate that is stable to the projected reactions; and the protective group must be selectively removable in good yield by readily available, preferably nontoxic reagents that do not attack the functional group(s) generated in such projected reactions (see Protective Groups in Organic Synthesis, 3 nd ed. T. W. Greene and P. G. M. Wuts, John Wiley & Sons, New York, N.Y., 1999).
  • protecting groups include, but are not limited to: benzoyl, substituted benzoyl, acetyl, phenyl-substituted benzoyl, trityl, DMT (4,4'-dimethoxytrityl), MMT (4- monomethoxytrityl), pixyl (9-phenylxanthen-9-yl) group, thiopixyl (9- phenylthioxanthen-9-yl).
  • the substituted benzoyl groups can be partially substituted or fully substituted.
  • the 2-, 3-, 4-, 5-, and 6-positions of the benzoyl ring can be substituted with either hydrogen (an unsubstituted position) or another substituent (a substituted position), such as the substituents contemplated above and throughout the present disclosure.
  • substituted benzoyl groups include, but are not limited to: 2-halo-benzoyl, 3-halo- benzoyl, 4-halo-benzoyl; 2,4-dihalo-benzoyl, 3,4-dihalo-benzoyl, and 3,5-dihalo- benzoyl; 2-(Ci_6-alkyl)-benzoyl, 3-(Ci_6-alkyl)-benzoyl, and 4-(Ci_6-alkyl)-benzoyl; 2,4-(diCi_ 6 -alkyl)-benzoyl, 3,4-(diCi_ 6 -alkyl)-benzoyl, and 3,5-(diCi_ 6 -alkyl)- benzoyl; 2-nitro-benzoyl, 3-nitro-benzoyl, 4-nitro-benzoyl; 2,4-(dinitro)-benzoyl, 3,4-(dinitro)-benzoyl, and 3,5-
  • leaving group (see also "Lv") as used herein, has the same meaning to the skilled artisan (Advanced Organic Chemistry: reactions, mechanisms and structure-Fourth Edition by Jerry March, John Wiley and Sons Ed.; 1992 pages 351-357) and represents a group which is part of and attached to a substrate molecule; in a reaction where the substrate molecule undergoes a displacement reaction (with for example a nucleophile), the leaving group is then displaced.
  • leaving groups include, but are not limited to: halogen (F, Cl, Br, and I), preferably Cl, Br, or I; tosylate, mesylate, triflate, acetate, etc.
  • Hydride reducing agents include, but are not limited to: ('BuO ⁇ AlH, sodium (bis(2-methoxyethoxy)(2,2,2-trifluoro-ethoxy)aluminum hydride, Red- Al (sodium bis(2-methoxyethoxy)aluminum hydride), sodium borohydride, lithium aluminum hydride, diborane, borane-tetrahydrofuran complex, borane-dimethylsulfide complex, aluminum tri-isopropoxide, boron triacetoxy hydride, alcohol dehydrogenase enzymes, (-)- or (+)- diisopinocampheylchloroborane, lithium (2,3-Dimethyl-2-butyl)-t- butoxyborohydride, Diisobuty
  • the term "reagent" standing alone, as used herein, has the meaning of at least one compound capable of reacting with the lactol derivative by introducing a leaving group at the anomeric carbon atom.
  • the at least one compound includes, but is not limited to, Ph 3 P/CBr 4 , Ph 3 P/CHBr 3 , Ph 3 P/CHBr 3 /imidazole Ph 3 P/Br 2 , Ph 3 P/Br 2 /imidazole, N-bromosuccinimide/ Ph 3 P, HBr in acetic acid, PBr 3 ZDMF, PBr 3 /sodium bicarbonate, PBr 3 /imidazole, PBrs/DMF, PBrs/sodium bicarbonate, PBrs/imidazole, N-chlorosuccinimide/Pr ⁇ P, POBr 3 /imidazole, POCl 3 /imidazole, SOCl 2 , SO 2 Cl 2 ,
  • basic reagent means a compound that is capable of abstracting a proton from an acidic reagent, such as a purine base, whereby the "acidic" functional group of the purine base includes the N-H of the fused imidazole ring.
  • Examples of basic reagents include, but are not limited to, a (lower alk)oxide ((lower alkyl)OM) in combination with an alcoholic solvent, where (lower alk)oxides include, but are not limited to, MeO “ , EtO “ , “PrO “ , 'PrO “ , 'BuO “ , 'AmO- (iso-amyloxide), etc., and where M is an alkali metal cation, such as Li + , Na + , K + , etc.
  • Alcoholic solvents include (lower alkyl)OH, such as, for example, MeOH, EtOH, "PrOH, 'PrOH, 'BuOH, 'AmOH, etc.
  • Non-alkoxy bases can also be used such as sodium hydride, sodium hexamethyldisilazane, lithium hexamethyldisilazane, lithium diisopropylamide, calcium hydride, sodium carbonate, potassium carbonate, cesium carbonate, DBU, and DBN.
  • nucleophilic reagent means a compound that contains a radical that is capable of replacing another radical, e.g., by way of a nucleophilic-substitution reaction.
  • An example of a nucleophilic reagent includes, but is not limited to, a (lower alk)oxide ((lower alkyl)OM) in combination with an alcoholic solvent, where (lower alk)oxides include, but are not limited to, MeO " , EtO " , "PrO " , 'PrO " , 'BuO " , etc., and where M is an alkali metal cation, such as Li + , Na + , K + , etc.
  • Alcoholic solvents include (lower alkyl)OH, such as, for example, MeOH, EtOH, "PrOH, 'PrOH, 'BuOH, etc.
  • a nucleophilic reagent includes, but is not limited to, an (aralkyl)oxide in combination with an aralkanol solvent, such as, for example, BnONa/BnOH, where "Bn” represents a benzyl radical (CeH 5 CH 2 -).
  • nucleophilic reagent includes, but is not limited to, an unsubstituted or substituted heterocycle containing carbon, hydrogen, and at least one of N, O, and S, where the C and N can be trivalent or tetravalent, i.e., sp 2 - or sp 3 -hybridized in the presence of a basic reagent or lower alkyl amine, such as triethylamine or diisopropyl ethyl amine, etc.
  • heterocycles include (see also a preceding paragraph), but are not limited to, aziridine, azetidine, pyrrolidine, piperidine, imidazole, oxazole, piperazine, etc.
  • nucleophilic reagents includes primary and secondary amines, which includes, but is not limited to, H 2 NR' or HNR' 2 , wherein R' is an optionally substituted alkyl, which includes, but is not limited to, an optionally substituted Ci_ 2 o alkyl, an optionally substituted C 1-10 alkyl, an optionally substituted lower alkyl; or an optionally substituted cycloalkyl.
  • nucleophilic reagent also provides for functional groups, which when introduced at the 6 position of the purine moiety, are capable of being co verted to a hydroxy group.
  • a group capable of being converted to OH means a substituent comprised of -OZ, where -OZ is converted to -OH on exposure to certain chemical reagents.
  • P(III)-reagent as used in the process described herein means a chemical reagent whereby the phosphorus atom has a +3-oxidation state.
  • P(III)-reagents examples include, but are not limited to, P(Lv) 3 , R 7 OP(Lv) 2 , R 7 OP(Lv)(N(C i_6 alkyl) 2 ), R 7 OP[N(d_ 6 alkyl) 2 ] 2 , and mixtures thereof, where Lv is a leaving group, R 7 and Ci_6 alkyl are defined herein.
  • activator reagent as used in the process described herein means a compound that promotes the reaction involving a P(III)-reagent.
  • activators include, but are not limited to, lH-tetrazole, 5-ethylthiotetrazole, imidazolium triflate, and 4,5-dicyano-imidazole, as well as those disclosed in U.S. Patent No. 6,274,725.
  • P(V)-reagents include, but are not limited to, P(O)(Lv) 3 , R 7 OP(O)(Lv) 2 , R 7 OP(O)(LV)(N(CL 6 alkyl) 2 ), R 7 OP(O)[N(CL 6 alkyl) 2 ] 2 , and mixtures thereof, where Lv is a leaving group, R 7 and C L6 alkyl are defined herein.
  • oxidizing agent as used in the process described herein means a chemical reagent that increases the oxidation state of an atom, i.e., an oxidizing agent promotes "oxidizing" or "oxidation.”
  • the atom that is oxidized is phosphorus, as in a phosphite derivative of II.
  • oxidizing agents include, but are not limited to, hydrogen peroxide, hydroperoxides, peroxides, peracids, iodine, and mixtures thereof.
  • Hydrogen peroxide can be used in the presence of a solvent, such as acetonitrile, as disclosed in Cvetovich, R. J. Organic Process Research & Development, Article ASAP, Publication Date (Web): May 11, 2009.
  • Hydroperoxides include peroxides in which R is an alkyl or an aryl and salts thereof, which include, but is not limited to t-butylperoxide ('BuOOH).
  • Peroxides include alkyl, aryl, or mixed alkyl/aryl peroxides and salts thereof.
  • Peracids include alkyl and aryl peracids, which include, but are not limited to, m-chloroperoxybenzoic acid (mCPBA).
  • an elemental halogent such as, bromine (Br 2 ), chlorine (Cl 2 ), or iodine (I 2 )
  • bromine (Br 2 ) chlorine (Cl 2 ), or iodine (I 2 )
  • Cl 2 iodine
  • water and other components such as, pyridine, tetrahydrofuran, and water.
  • an aqueous Cl 2 solution in the presence of TEMPO is contemplated as well.
  • equilibrating the phosphite derivative of II refers to a process where a composition comprising the two isomers (cis and trans) of the phosphite derivative of II is allowed to equilibrate, as depicted in the following equation.
  • cis and trans refer to the spatial position of the -OR 7 substituent relative to the spatial position of the nucleobase on the furanose ring system.
  • the equilibrium position i.e., the ratio of cis-to- trans
  • solvent, temperature, etc. may be influenced by solvent, temperature, etc. and that the conditions to obtain a certain equilibrium position can be determined experimentally, such experimental techniques include, but are not limited to, 1 H- or 31 P-NMR spectroscopy.
  • amine reagent as used herein means a composition containing at least one compound comprising nitrogen in its protonated or unprotonated form.
  • alkyl amines such as methyl amine, di-isopropyl amine, cyclopentyl- amine, as well as heterocyclic compounds that include, but are not limited to, pyrrolidine, piperidine, morpholine, etc., imidazole, N-(C 1-6 alkyl)-imidazole, such as, N-methyl-imidazole, pyrazole, N-(C 1-6 alkyl)- pyrazole, such as, N-methyl- pyrazole, triazole, N-(C 1-6 alkyl)-triazole, such as N-methyl-triazole, tetrazole, N- (C 1-6 alkyl)-tetrazole, such as N-methyl-tetrazole, oxazole, etc.
  • heterocyclic amines are disclosed in T. L. Gilchrist, Heterocyclic Chemistry, 1985, Longman Scientific & Technical, which is hereby incorporated by reference.
  • purine or "derivatized purine", as used in the process described herein, means, in addition to the compounds specifically disclosed in a preceding paragraph, a precursor compound of the "Base” of the compounds represented by the structures of formula I and formula II.
  • salts thereof includes acid or basic additions salts, as well as pharmaceutically acceptable salts.
  • pharmaceutically acceptable salt means a salt that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound.
  • Such salts include, but are not limited to: (1) acid addition salts, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as gly colic acid, pyruvic acid, lactic acid, malonic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, 3-(4- hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1 ,2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 4-chlorobenzen
  • references to pharmaceutically acceptable salts include solvent addition forms (solvates) or crystal forms (polymorphs) as defined herein, of the same acid addition salt.
  • preparation or “dosage form” is intended to include both solid and liquid formulations of the active compound and one skilled in the art will appreciate that an active ingredient can exist in different preparations depending on the desired dose and pharmacokinetic parameters.
  • excipient refers to a compound that is used to prepare a pharmaceutical composition, and is generally safe, non-toxic and neither biologically nor otherwise undesirable, and includes excipients that are acceptable for veterinary use as well as human pharmaceutical use.
  • crystalline refers to a situation where a solid sample of compound I or II has crystalline characteristics when determined by X-ray powder diffraction or a single crystal X-ray technique.
  • crystal-like refers to a situation where a solid sample of compound I or II has crystalline characteristics when determined by one means, e.g., visually or by optical or polarizing microscopy, but does not have crystalline characteristics when determined by another means, e.g., x-ray powder diffraction.
  • Methods of visually determining the crystallinity of a solid sample by visual or by optical or by polarizing microscopy are disclosed in USP ⁇ 695> and ⁇ 776>, both of which are incorporated by reference.
  • a solid sample of compound I or II that is "crystal-like” may be crystalline under certain conditions but may become noncrystalline when subjected to other conditions.
  • amorphous refers to a situation where a solid sample of compound I or II is neither crystalline nor crystal-like.
  • co-crystallates include co-crystallates of compound I or II in combination with salts, which embraces pharmaceutically acceptable salts.
  • substantially anhydrous means that a substance contains at most
  • a (lattice or adsorbed) solvent (designated in some instances by the symbol S) or anti-solvent includes at least one of a Ci to Cg alcohol, a C 2 to Cg ether, a C3 to C 7 ketone, a C3 to C 7 ester, a Ci to C 2 chlorocarbon, a C5 to Ci 2 saturated hydrocarbon, and a C 6 to Ci 2 aromatic hydrocarbon.
  • R 1 is hydrogen, n-alkyl; branched alkyl; cycloalkyl; or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of
  • Ci_6 alkyl C 2 _6 alkenyl, C 2 _6 alkynyl, Ci_6 alkoxy, F, Cl, Br, I, nitro, cyano, C 1-6 haloalkyl, -N(R r ) 2 , C 1-6 acylamino, -NHSO 2 CL 6 alkyl, -SO 2 N(R r ) 2 , COR 1 , and -SO 2 Ci_6 alkyl;
  • R 1 is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 20 alkyl, C 1-10 alkyl, or Ci_ 6 alkyl, R 1 is -
  • R 2 is hydrogen, C 1-10 alkyl, or R 3a or R 3b and R 2 together are (CH 2 ) n so as to form a cyclic ring that includes the adjoining N and C atoms, where n is 2 to
  • R 3a and R 3b both are C 1-6 alkyl
  • R 3a and R 3b together are(CH 2 ) f so as to form a spiro ring;
  • R 3a is hydrogen and R 3b and R 2 together are (CH 2 ) n so as to form a cyclic ring that includes the adjoining N and C atoms
  • R 3b is hydrogen and R 3a and R 2 together are (CH 2 ) n so as to form a cyclic ring that includes the adjoining N and C atoms, where c is 1 to 6, d is 0 to 2, e is 0 to 3, f is 2 to 5, n is 2 to 4, and where R 3 is independently hydrogen or C 1-6 alkyl and R 3" is -OR or -N(R 3' ) 2 );
  • R 3a is H and R 3b is H, CH 3 , CH 2 CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 -indol-3-yl, -CH 2 CH 2 SCH 3 , CH 2 CO 2 H, CH 2 C(O)NH 2 , CH 2 CH 2 COOH, CH 2 CH 2 C(O)NH 2 , CH 2 CH 2 CH 2 CH 2 NH 2 , - CH 2 CH 2 CH 2 NHC(NH)NH 2 , CH 2 -imidazol-4-yl, CH 2 OH, CH(OH)CH 3 , CH 2 ((4'-OH)-Ph), CH 2 SH, or lower cycloalkyl; or
  • R 3a is CH 3 , -CH 2 CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 -indol-3-yl, -CH 2 CH 2 SCH 3 , CH 2 CO 2 H, CH 2 C(O)NH 2 , CH 2 CH 2 COOH, CH 2 CH 2 C(O)NH 2 , CH 2 CH 2 CH 2 CH 2 NH 2 , - CH 2 CH 2 CH 2 NHC(NH)NH 2 , CH 2 -imidazol-4-yl, CH 2 OH, CH(OH)CH 3 , CH 2 ((4'-OH)-Ph), CH 2 SH, or lower cycloalkyl and R 3b is H, where R 3' is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 2 o alkyl, C LIO alkyl, or C 1-6 alkyl, R 3" is -OR'
  • R 4 is hydrogen, C 1-10 alkyl, C 1-10 alkyl optionally substituted with a lower alkyl, alkoxy, di(lower alkyl)-amino, or halogen, C 1-10 haloalkyl, C 3 _io cycloalkyl, cycloalkyl alkyl, cycloheteroalkyl, aminoacyl, aryl, such as phenyl, heteroaryl, such as, pyridinyl, substituted aryl, or substituted heteroaryl;
  • R 5 is H, a lower alkyl, CN, vinyl, O-(lower alkyl), hydroxyl lower alkyl, i.e., -(CH 2 ) p 0H, where p is 1 -6, including hydroxyl methyl (CH 2 OH), CH 2 F, N 3 , CH 2 CN, CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , alkyne (optionally substituted), or halogen, including F, Cl, Br, or I;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , F, CN, vinyl, or ethynyl;
  • R 7 is hydrogen, n-alkyl, branched alkyl, cycloalkyl, alkaryl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkenyl Of C 2 -C 6 , lower alkynyl Of C 2 -C 6 , such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy of Ci-C 6 , halogenated (F, Cl, Br, I
  • R 5 , R 6 , and X have their meanings as described above, X' is a leaving group, and R 11 is a protecting group.
  • a first embodiment is directed to compound I,
  • R 1 is hydrogen, n-alkyl; branched alkyl; cycloalkyl; or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of Ci_ 6 alkyl, C 2 - 6 alkenyl, C 2 _ 6 alkynyl, Ci_ 6 alkoxy, F, Cl, Br, I, nitro, cyano, Ci_ 6 haloalkyl, -N(R 1 ) ⁇ Ci_ 6 acylamino, - NHSO 2 C L6 alkyl, -SO 2 N(R r ) 2 , COR 1" , and -SO 2 C 1-6 alkyl;
  • R 1' is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 2 o alkyl, C 1-10 alkyl, or C 1-6 alkyl, R 1" is -OR' or -N(
  • R 2 is hydrogen or CH 3 ;
  • R 3a and R 3b are
  • R 3a is CH 3 , -CH 2 CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 -indol-3-yl, -CH 2 CH 2 SCH 3 , CH 2 CO 2 H, CH 2 C(O)NH 2 , CH 2 CH 2 COOH, CH 2 CH 2 C(O)NH 2 , CH 2 CH 2 CH 2 CH 2 NH 2 , -
  • R 3b is H, where R 3' is independently hydrogen or alkyl, which includes, but is not limited to, Ci_ 2 o alkyl, C 1-10 alkyl, or Ci_ 6 alkyl, R 3" is -OR' or -N(R 3' ) 2 );
  • R 4 is hydrogen, C 1-10 alkyl, C 1-10 alkyl optionally substituted with a lower alkyl, alkoxy or halogen, C 1-10 haloalkyl, C 3 _io cycloalkyl, cycloalkyl alkyl, cycloheteroalkyl, aminoacyl, di(lower alkyl)amino-lower alkyl, aryl, such as phenyl, heteroaryl, such as, pyridinyl, substituted aryl, or substituted heteroaryl;
  • R 5 is H, a lower alkyl, CN, vinyl, O-(lower alkyl), hydroxyl lower alkyl, i.e., -(CH 2 ) p 0H, where p is 1 -6, including hydroxyl methyl (CH 2 OH), CH 2 F, N 3 , CH 2 CN, CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , alkyne (optionally substituted), or halogen, including F, Cl, Br, or I;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl, ethynyl;
  • X is H, OH, OMe, CN, F, Cl, Br, I, NH 2 , or N 3 ;
  • O) Z is N or CR 10 ;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl);
  • R 1 is hydrogen, methyl, ethyl, n-propyl, /-propyl, phenyl, p-tolyl, p- bromo-phenyl, p-chloro-phenyl, p-fluorophenyl;
  • R 2 is hydrogen or CH 3 ;
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 -indol-3-yl, -CH 2 CH 2 SCH 3 , CH 2 CO 2 H, CH 2 C(O)NH 2 , CH 2 CH 2 COOH, CH 2 CH 2 C(O)NH 2 , CH 2 CH 2 CH 2 CH 2 NH 2 , -
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, N-methyl-aziridin-2-yl, N-methyl- azetidin-3-yl, N-methyl-pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl- piperidin-4-yl, lower haloalkyl, or di(lower alkyl)amino-lower alkyl;
  • R 5 is H, CN, CH 3 , OCH 3 , CH 2 OH, CH 2 F, N 3 , halogen, including F, Cl, Br, or I;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl, or ethynyl;
  • X is H, OH, OCH 3 , CN, F, Cl, Br, I, NH 2 , or N 3 ;
  • O) Z is N or CR 10 ;
  • R 10 is an H, halogen (including F, Cl, Br, I), OR, NH 2 , NHR', NR 2 ,
  • R is a lower alkyl, a lower cycloalkyl, a lower alkyaryl, or a C(O)(lower alkyl).
  • R 1 is hydrogen, methyl, ethyl, n-propyl, /-propyl, phenyl, p-tolyl, p- bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl;
  • R 2 is hydrogen or CH 3 ;
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 ,
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, N-methyl-aziridin-2-yl, N-methyl- azetidin-3-yl, N-methyl-pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl- piperidin-4-yl, lower haloalkyl, or di(lower alkyl)amino-lower alkyl;
  • R 5 is H, CN, CH 2 F, N 3 , F, Cl, Br, or I;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , F, vinyl, or ethynyl;
  • X is H, OH, OCH 3 , CN, F, Cl, Br, I, NH 2 or N 3 ;
  • O) Z is N or CR 10 ;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl).
  • a third aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, methyl, ethyl, n-propyl, /-propyl, phenyl, p-tolyl, p- bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl;
  • R 2 is hydrogen
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, or lower cycloalkyl;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, N-methyl-aziridin-2-yl, N-methyl- azetidin-3-yl, N-methyl-pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl- piperidin-4-yl, lower haloalkyl, or di(lower alkyl)amino-lower alkyl;
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, vinyl or ethynyl;
  • X is H, OH, OCH 3 , CN, F, NH 2 or N 3 ;
  • O) Z is N or CR 10 ;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkyaryl, or a C(O)(lower alkyl).
  • a fourth aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, methyl, phenyl, p-bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl;
  • R 2 is hydrogen
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, or lower cycloalkyl;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, N-methyl-aziridin-2-yl, N-methyl- azetidin-3-yl, N-methyl-pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl- piperidin-4-yl, lower haloalkyl, or di(lower alkyl)amino-lower alkyl;
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, vinyl, or ethynyl;
  • X is H, OH, OCH 3 , CN, F, NH 2 or N 3 ;
  • O) Z is N or CR 10 ;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkyaryl, or a
  • a fifth aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, methyl, phenyl, p-bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl;
  • R 2 is hydrogen
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, or lower cycloalkyl;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, N-methyl-aziridin-2-yl, N-methyl- azetidin-3-yl, N-methyl-pyrrolidin-3-yl, N-methyl-pyrrolidin-4-yl, N-methyl- piperidin-4-yl, lower haloalkyl, or di(lower alkyl)amino-lower alkyl;
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, vinyl, or ethynyl;
  • X is H, OH, OCH 3 , CN, F, NH 2 or N 3 ;
  • Y is OH;
  • Z is N or CR 10 ;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkyl, or a C(O)(lower alkyl).
  • a sixth aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, phenyl, p-tolyl, p-bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl;
  • R 2 is hydrogen or CH 3 ;
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , CH(CH 3 )CH 2 CH 3 , CH 2 Ph, CH 2 OH, CH 2 ((4'-OH)-Ph), or lower cycloalkyl;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl;
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl or ethynyl;
  • X is H, OH, OCH 3 , CN, F, Cl, Br, I, NH 2 , or N 3 ;
  • R 10 is an H, F, Cl, Br, I, OR, NH 2 , NHR', NR 2 , or lower alkyl of C 1 - C 6 , or -C ⁇ CH;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl).
  • a seventh aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, phenyl, p-tolyl, p-bromo-phenyl, or p-chloro-phenyl;
  • R 2 is hydrogen or CH 3 ;
  • R 3a is H and R 3b is H, CH 3 , CH(CHs) 2 , CH 2 CH(CHs) 2 ,
  • R 4 is hydrogen, CH 3 , Et, 'Pr, “Pr, “Bu, 2-butyl, 'Bu, benzyl, cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl;
  • R 5 is H ofN 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl or ethynyl;
  • X is H, OH, OCH 3 , CN, F, Cl, Br, or N 3 ;
  • R 9 is NH 2 and R 8 is H, F, OH, O(lower alkyl), O(lower alkyaryl), NH 2 , NHR', NR 2 , or nitrogen heterocycle;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl).
  • An eighth aspect of the first embodiment is directed to compound I,
  • R 1 is hydrogen, phenyl, p-bromo-phenyl, p-chloro-phenyl, or p- fluorophenyl;
  • R 2 is hydrogen
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , CH 2 CH(CH 3 ) 2 , or CH(CH 3 )CH 2 CH 3 ;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, "Pr, “Bu, 2-butyl, or cyclopentyl;
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl, or ethynyl;
  • X is H, OH, CN, F, or N 3 ;
  • R 9 is NH 2 and R 8 is H, F, OH, O(lower alkyl), O(lower alkaryl), NH 2 , NHR, NR' 2 , or nitrogen heterocycle;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl).
  • R 1 is hydrogen, phenyl, p-bromo-phenyl, p-chloro-phenyl, or p- fluorophenyl;
  • R 2 is hydrogen
  • R 3a is H and R 3b is H, CH 3 , CH(CH 3 ) 2 , or CH 2 CH(CH 3 ) 2 ;
  • R 4 is hydrogen, CH 3 , Et, 'Pr, "Pr, “Bu, 2-butyl, or cyclopentyl;
  • R 5 is H
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, CN, vinyl, or ethynyl
  • X is H, OH, CN, F, or N 3 ;
  • R 9 is NH 2 and R 8 is H, F, OH, O(lower alkyl), O(lower alkaryl), NH 2 , NHR, NR' 2 , or nitrogen heterocycle;
  • R is a lower alkyl, a lower cycloalkyl, a lower alkaryl, or a C(O)(lower alkyl).
  • a tenth aspect of the first embodiment is directed to compound I wherein (a) R 1 is hydrogen, phenyl, p-bromo-phenyl, p-chloro-phenyl, or p-fluorophenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ; (d) R 4 is hydrogen, CH 3 , 'Pr, or cyclopentyl; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; (i) R 8 is independently OH, OMe, OEt, O'Pr, OBn, or-N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl), and R 9 is NH 2 ; O) Z is N.
  • An eleventh aspect of the first embodiment is directed to compound I wherein (a) R 1 is phenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ;(d) R 4 is 'Pr; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; R 8 is OMe; and (j) Z is N.
  • a twelfth aspect of the first embodiment is directed to compound I wherein (a) R 1 is phenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ;(d) R 4 is 'Pr; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; R 8 is OEt; and O) Z is N.
  • a thirteenth aspect of the first embodiment is directed to compound I wherein (a) R 1 is phenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ;(d) R 4 is 'Pr; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; R 8 is O'Pr; and O) Z is N.
  • a fourteenth aspect of the first embodiment is directed to compound I wherein (a) R 1 is phenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ;(d) R 4 is CH 3 ; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; R 8 is -N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl), and R 9 is NH 2 ; and O) Z is N.
  • a fifteenth aspect of the first embodiment is directed to compound I wherein (a) R 1 is phenyl; (b) R 2 is hydrogen; (c) R 3a is H and R 3b is CH 3 ;(d) R 4 is cyclopentyl; (e) R 5 is H; (f) R 6 is CH 3 ; (g) X is F; (h) Y is OH; R 8 is independently OBn; and R 9 is NH 2 ; and G) Z is N.
  • R 1 is phenyl
  • R 2 is hydrogen
  • R 3a is H and R 3b is CH 3
  • R 4 is cyclopentyl
  • R 5 is H
  • R 6 is CH 3
  • X is F
  • Y is OH
  • R 8 is OH and R 9 is NH 2
  • O Z is N.
  • R 4 is a lower alkyl and R 8 is a O(lower alkyl).
  • R 8 is a O(lower alkyl).
  • preferred compounds include:
  • compound 11 is particularly preferred.
  • 11 comprises a mixture of diastereomers designated Sp-11 and i?p-ll.
  • Contemplated herein is a composition that comprises Sp-Il, i?p-ll, or mixtures thereof.
  • the composition that comprises Sp-H, i?p-ll, or mixtures thereof can also be part of a solvate, a hydrate, or a mixed solvate/hydrate.
  • the solvate is designated as iSp-1 l-nS, R ⁇ -ll-nS, or 11 -nS; while the hydrate is designated as Sv-H mH 2 O, R ⁇ - 11 -HiH 2 O, or ll-mH 2 0, where S is a lattice solvent, n varies by an integer or non- integer amount from about 0 to about 3 and m varies by an integer or non-integer amount from about 0 to about 5.
  • the composition that comprises Sp-ll, i?p-ll, or mixtures thereof and its salts, solvates, and hydrates thereof is crystalline, crystal- like, or amorphous.
  • a second embodiment is directed to compound II,
  • R 5 is H, a lower alkyl, cyano (CN), vinyl, O-(lower alkyl), including OCH 3 , OCH 2 CH 3 , hydroxyl lower alkyl, i.e., -(CH 2 ) P OH, where p is 1 -6, including hydroxyl methyl (CH 2 OH), fluoromethyl (CH 2 F), azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , F, vinyl, or ethynyl;
  • R 7 is H, n-alkyl, branched alkyl, cycloalkyl, alkaryl, alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I), halogenated (F, Cl, Br, I) lower alkenyl of C 2 -C 6 , lower alkynyl of C 2 -C 6 such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy of
  • X is H, OH, F, OMe, NH 2 , or N 3 ;
  • R 8 and R 9 are independently H, F, Cl, Br, I, OH, OR', SH, SR', NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 _ q X q , where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 H, CO 2 CH 3 , CONH 2 , CONHCH 3 , CON(CH 3 ) 2 , (f) Z is N or CR 10 ; and
  • R is a Ci_ 2 o alkyl; a Ci_ 2 o cycloalkyl; an alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a first aspect of the second embodiment is directed to compound II,
  • R 5 is H, a lower alkyl, cyano (CN), vinyl, O-(lower alkyl), including OCH 3 , OCH 2 CH 3 , hydroxyl lower alkyl, i.e., -(CH 2 ) P OH, where p is 1 -6, including hydroxyl methyl (CH 2 OH), fluoromethyl (CH 2 F), azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , CH 2 CH 3 , or F;
  • R 7 is H, n-alkyl, branched alkyl, cycloalkyl, alkaryl, alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I), halogenated (F, Cl, Br, I) lower alkenyl Of C 2 -C 6 , lower alkynyl Of C 2 -C 6 such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy Of Ci-
  • X is H, OH, F, OMe, NH 2 , or N 3 ;
  • R 8 and R 9 are independently H, F, Cl, Br, I, OH, OR', SH, SR', NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 .
  • q X q where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 H, CO 2 CH 3 , CONH 2 , CONHCH 3 , or CON(CH 3 ) 2 ,
  • (f) Z is N or CR 10 ;
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , NR" 2 , lower alkyl of C 1 -
  • R is a Ci_ 2 o alkyl; a Ci_ 2 o cycloalkyl; an alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a second aspect of the second embodiment is directed to compound II,
  • R 5 is H, a lower alkyl, cyano (CN), vinyl, O-(lower alkyl), including OCH 3 , OCH 2 CH 3 , hydroxyl lower alkyl, i.e., -(CH 2 ) P OH, where p is 1 -6, including hydroxyl methyl (CH 2 OH), fluoromethyl (CH 2 F), azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , or CH 2 CH 3 ;
  • R 7 is H, n-alkyl, branched alkyl, cycloalkyl, alkaryl, alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I), halogenated (F, Cl, Br, I) lower alkenyl of C 2 -C 6 , lower alkynyl of C 2 -C 6 such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy of
  • X is H, OH, F, OMe, NH 2 , or N 3 ;
  • R 8 and R 9 are independently H, F, Br, OH, OR, NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 _ q X q , where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 CH 3 , CONH 2 , CONHCH 3 , CON(CH 3 ) 2 ;
  • (f) Z is N or CR 10 ;
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl Of Ci-C 6 , or -C ⁇ CH;
  • R is a Ci_ 2 o alkyl; a Ci_ 2 o cycloalkyl; an alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a third aspect of the second embodiment is directed to compound II,
  • R 5 is H, a lower alkyl, cyano (CN), vinyl, O-(lower alkyl), including OCH 3 , OCH 2 CH 3 , hydroxyl lower alkyl, i.e., -(CH 2 ) P OH, where p is 1 -6, including hydroxyl methyl (CH 2 OH), fluoromethyl (CH 2 F), azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H, CH 3 , CH 2 F, CHF 2 , CF 3 , or CH 2 CH 3 ;
  • R 7 is H, n-alkyl, branched alkyl, cycloalkyl, alkaryl, alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I), halogenated (F, Cl, Br, I) lower alkenyl of C 2 -C 6 , lower alkynyl of C 2 -C 6 such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy of
  • X is F
  • R 8 and R 9 are independently H, F, Br, OH, OR, NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 _ q X q , where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 CH 3 , CONH 2 , CONHCH 3 , or CON (CH 3 ) 2 ;
  • (f) Z is N or CR 10 ;
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl Of Ci-C 6 , or -C ⁇ CH;
  • R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a fourth aspect of the second embodiment is directed to compound II,
  • R 5 is H, -CH 3 , cyano (CN), vinyl, -OCH 3 , -CH 2 OH, -CH 2 F, azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H, CH 3 , or CH 2 CH 3 ;
  • R 7 is H, n-alkyl, branched alkyl, cycloalkyl, alkaryl, alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkyl Of Ci-C 6 , halogenated (F, Cl, Br, I), halogenated (F, Cl, Br, I) lower alkenyl Of C 2 -C 6 , lower alkynyl Of C 2 -C 6 such as C ⁇ CH, halogenated (F, Cl, Br, I) lower alkynyl Of C 2 -C 6 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy of Ci-
  • R 8 and R 9 are independently H, F, OH, OR, SH, SCH 3 , NH 2 , NHR',
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl Of Ci-C 6 , or -C ⁇ CH;
  • R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a fifth aspect of the second embodiment is directed to compound II,
  • R 5 is H, -CH 3 , cyano (CN), vinyl, -OCH 3 , -CH 2 OH, -CH 2 F, azido (N 3 ), CH 2 CN, CH 2 N 3 , CH 2 NH 2 , CH 2 NHCH 3 , CH 2 N(CH 3 ) 2 , F, Cl, Br, or I
  • R 6 is H or CH 3 ;
  • R 7 is H, lower alkyl, lower alkylaryl, lower cycloalkyl, lower alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR 7' 2 , lower alkoxy Of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkoxy Of Ci-C 6 , wherein R 7 is an optionally substituted alkyl, cycloalkyl, alkenyl, alkynyl, or alkoxyalkyl, which includes, but is not limited to, Ci_io alkyl, C 3 _ 7 cycloalkyl, C 2 _io alkenyl, C 2 _io alkynyl, or Ci_io
  • R 8 and R 9 are independently H, F, OH, OR', NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 _ q X q , where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 H, CO 2 CH 3 , CONH 2 , CONHCH 3 , CON(CH 3 ) 2 ;
  • (f) Z is N or CR 10 ;
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl Of Ci-C 6 , or -C ⁇ CH;
  • R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a sixth aspect of the second embodiment is directed to compound II,
  • R 5 is H or N 3 ;
  • R 6 is H, CH 3 , or CH 2 CH 3 ;
  • R 7 is H, lower alkyl, lower alkylaryl, lower cycloalkyl, lower alkenyl, or aryl, which includes, but is not limited to, phenyl or naphthyl, where phenyl or naphthyl are optionally substituted with at least one of H, F, Cl, Br, I, OH, OR 7' , SH, SR 7' , NH 2 , NHR 7' , NR T 2 , lower alkoxy of C 1 -C 6 , halogenated (F, Cl, Br, I) lower alkoxy of Ci-C 6 , wherein R 7 is an optionally substituted alkyl, cycloalkyl, alkenyl, alkynyl, or alkoxyalkyl, which includes, but is not limited to, C 1-10 alkyl, C 3 _7 cycloalkyl, C 2-10 alkenyl, C 2-10 alkynyl, or C 1-10 alkoxyalkyl;
  • R 8 and R 9 are independently H, F, OH, OR', NH 2 , NHR, NR' 2 , nitrogen heterocycle, CH 3 , CH 3 _ q X q , where X is F, Cl, Br, or I and q is 1 to 3, vinyl, CO 2 H, CO 2 CH 3 , CONH 2 , CONHCH 3 , CON(CH 3 ) 2 ,
  • (f) Z is N or CR 10 ;
  • R 10 is an H, F, OH, OR, NH 2 , NHR', NR 2 , lower alkyl of Ci-C 6 , halogenated (F, Cl, Br, I) lower alkyl Of Ci-C 6 , or -C ⁇ CH;
  • R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • a seventh aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is H, CH 3 , or CH 2 CH 3 ; (c) R 7 is lower alkyl or lower cycloalkyl; (d) X is F; (e) R 8 and R 9 are independently H, F, OH, OR', NH 2 , NHR, NR' 2 , nitrogen heterocycle, and (f) Z is N; wherein R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 -C 6 alkenyl, a C 2 -C 6 alkynyl.
  • An eighth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is H, CH 3 , or CH 2 CH 3 ; (c) R 7 is lower alkyl or lower cycloalkyl; (d) X is F; (e) R 9 is NH 2 and R 8 OH, OR, or nitrogen heterocycle, and (f) Z is N; wherein R is a lower alkyl; a lower cycloalkyl; a lower alkaryl, a C 2 - Ce alkenyl, a C 2 -C 6 alkynyl.
  • a ninth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is H, CH 3 , or CH 2 CH 3 ; (c) R 7 is lower alkyl or lower cycloalkyl; (d) X is F; (e)R 9 is NH 2 and R 8 is O(lower alkyl), O(lower cycloalkyl), or nitrogen heterocycle; and (f) Z is N.
  • a tenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is is lower alkyl or lower cycloalkyl; (d) X is F; (e) R 9 is NH 2 and R 8 is O(lower alkyl), O(lower cycloalkyl), or nitrogen heterocycle; and (f) Z is N.
  • An eleventh aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is is lower alkyl or lower cycloalkyl; (d) X is F; (e) R 9 is NH 2 and R 8 is O(lower alkyl), O(lower cycloalkyl) or -N(- CH 2 CH 2 CH 2 -) (azetidin- 1 -yl); and (f) Z is N.
  • a twelfth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 9 is NH 2 and R 8 is O(lower alkyl) or -N(-CH 2 CH 2 CH 2 -) (azetidin- 1-yl); and (f) Z is N.
  • a thirteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 9 is NH 2 and R 8 is OMe, OEt, O'Pr, or -N(-CH 2 CH 2 CH 2 -) (azetidin- 1-yl); and (f) Z is N.
  • a fourteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 9 is NH 2 and R 8 is OMe, OEt, or O'Pr; and (f) Z is N.
  • a fifteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 8 is
  • a sixteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 8 is
  • OEt and R 9 is NH 2 ; and (f) Z is N.
  • a seventeenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 , 'Pr, c Bu, or c Pn; (d) X is F; (e) R 8 is O'Pr and R 9 is NH 2 ; and (f) Z is N.
  • An eighteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 ; (d) X is F; (e) R 8 is -N(- CH 2 CH 2 CH 2 -) (azetidin-1-yl); and R 9 is NH 2 ; and (f) Z is N.
  • a nineteenth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is CH 3 ; (d) X is F; (e) R 8 is OEt and R 9 is
  • a twentieth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is 'Pr; (d) X is F; (e) R 8 is OEt and R 9 is NH 2 ; and (f) Z is N.
  • a twenty-first aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is c Bu; (d) X is F; (e) R 8 is OMe and R 9 is
  • a twenty-second aspect of the second embodiment is directed to a compound II wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is c Pn; (d) X is F; (e) R 8 is OMe and R 9 is NH 2 ; and (f) Z is N.
  • a twenty-third aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is c Bu; (d) X is F; (e) R 8 is OEt and R 9 is
  • a twenty- fourth aspect of the second embodiment is directed to compound II, wherein (a) R 5 is H; (b) R 6 is CH 3 ; (c) R 7 is c Pn; (d) X is F; (e) R 8 is OEt and R 9 is
  • Preferred compounds to be prepared by way of the second embodiment, or by another suitable process include compounds represented by the following structure
  • R 7 is a lower alkyl or a lower cycloalkyl and R 8 is an O(lower alkyl).
  • R 8 is an O(lower alkyl).
  • preferred compounds include:
  • 17 is particularly preferred.
  • 17 comprises a mixture of diastereomers designated as Rp- 17 and Sp-17.
  • Contemplated herein is a composition that comprises Sp-17, i?p-17, or mixtures thereof. It is preferred that the composition comprises purified i?p-17. It is further preferred that the composition comprises substantially pure i?p-17.
  • the composition that comprises Sp- 17, i?p-17, or mixtures thereof can also be part of a solvate, a hydrate, or a mixed solvate/hydrate.
  • the solvate is designated as Sp-17-nS, i?p-17-nS, or 17-nS; while the hydrate is designated as S P -17 mH 2 O, R P - 17-HiH 2 O, or 17-mH 2 O, where S is a lattice solvent, n varies by an integer or non- integer amount from about O to about 3 and m varies by an integer or non-integer amount from about O to about 5.
  • composition that comprises Sp- 17, i?p-17, or mixtures thereof and its salts, solvates, and hydrates thereof is crystalline, crystal- like, or amorphous.
  • a first aspect of the preferred compounds of formula II is directed to crystalline i?p-17.
  • a second aspect of the preferred compounds of formula II directed to crystalline R P -l7 having an XRD 2 ⁇ -reflections (°) at about 12.2.
  • a third aspect of the preferred compounds of formula II directed to crystalline R P -ll having XRD 2 ⁇ -reflections (°) at about 12.2, 14.3, 15.5, and 19.9.
  • a fourth aspect of the preferred compounds of formula II directed to crystalline R 7 -Il having XRD 2 ⁇ -reflections (°) at about 12.2, 14.3, 15.5, 17.4, 18.1, 19.9, 22.8, 23.6, 24.5, 25.1, and 27.35.
  • a fifth aspect of the preferred compounds of formula II directed to crystalline i?p-17 having an XRD diffraction pattern substantially as that shown in Fig. 1.
  • a sixth aspect of the preferred compounds of formula II directed to orthorhombic, crystalline i?p-17.
  • a sixth aspect of the preferred compounds of formula II directed to crystalline i?p-17 having orthorhombic (P2i2i2i) unit cell parameters of a ⁇ 11.4 A, b ⁇ 12.4 A, and c ⁇ 14.2 A.
  • a seventh aspect of the preferred compounds of formula II directed to crystalline R P -ll having the following FT-IR peaks (cm 1 ): -1581, -1295, -1065, -999, -798, and ⁇ 79 ⁇
  • An eighth aspect of the preferred compounds of formula II is directed to crystalline i?p-17 having an FT-IR spectrum substantially as that shown in Fig. 2.
  • a ninth aspect of the preferred compounds of formula II is directed to substantially pure i?p-17.
  • a tenth aspect of the preferred compounds of formula II is directed to substantially pure, crystalline i?p-17.
  • An eleventh aspect of the preferred compounds of formula II is directed to substantially pure, crystal-like i?p-17.
  • a twelfth aspect of the preferred compounds of formula II is directed to substantially pure, amorphous i?p-17.
  • a third embodiment is directed to a process for preparing compound I or compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, wherein said process comprises:
  • R 5 , R 6 , and X have their meanings as described above, X' is a leaving group, and R 11 is a protecting group.
  • a first aspect of the third embodiment directed to a process for preparing a compound I or compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, wherein for the compound III or the compound IV, R 11 is benzoyl or substituted benzoyl (preferably R 11 is 4-chloro- benzoyl), R 5 is H, R 6 is CH3, and X is F.
  • the hydride reducing agent is ('BuO) 3 AlH, sodium (bis(2-methoxyethoxy)(2,2,2-trifluoro-ethoxy)aluminum hydride, or Red- Al (sodium bis(2-methoxyethoxy)aluminum hydride).
  • the stereoselective reduction can be performed in a solvent comprising tetrahydrofuran (THF) or diethyl ether, preferably the solvent is THF at a temperature ranging from about (-78 0 C) to about 25°C, preferably at a temperature ranging from about (-78 0 C) to about O 0 C, and most preferably at a temperature from about (-30 0 C) to about 0 0 C.
  • THF tetrahydrofuran
  • diethyl ether diethyl ether
  • a second aspect of the third embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, said process comprising stereoselective reduction of a protected ribonolactone III using a hydride reducing agent to provide a mixture comprising a beta-lactol derivative IV and an alpha-lactol derivative IV- ⁇ :
  • R 5 , R 6 , and X have the meanings as defined herein above and R 11 is a protecting group .
  • a third aspect of the third embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, wherein for the compound III, the compound IV, or the compound IV- ⁇ , R 5 is H, R 6 is CH 3 , R 11 is benzoyl or a substituted benzoyl (preferably R 11 is 4-chloro-benzoyl), and X is F.
  • the hydride reducing agent is ('BuO ⁇ AlH, sodium (bis(2-methoxyethoxy)(2,2,2-trifluoro- ethoxy)aluminum hydride, or Red- Al (sodium bis(2-methoxyethoxy)aluminum hydride).
  • the stereoselective reduction can be performed in a solvent comprising tetrahydrofuran (THF) or diethyl ether, preferably the solvent is THF at a temperature ranging from about (-78 0 C) to about 25°C, preferably at a temperature ranging from about (-78 0 C) to about 0 0 C, and most preferably at a temperature from about (-30 0 C) to about 0 0 C.
  • THF tetrahydrofuran
  • diethyl ether diethyl ether
  • the crystallization occurs by a process comprising adding to the mixture seed crystals of the beta-lactol derivative and then heating the mixture comprising the seed crystals of the beta-lactol derivative at a temperature that ranges from about 25°C to about 80 0 C, preferably about 50 0 C with (about 0.2 mmHg) or without applied vacuum.
  • a fourth aspect of the third embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, comprising stereoselective conversion of a lactol derivative IV using a reagent to obtain an anomeric alpha-derivative V
  • R 5 , R 6 , and X have the meanings as defined herein above, R 11 is a protecting group and X' is a leaving group.
  • a fifth aspect of the third embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, wherein for the compound IV or the compound V, R 5 is H, R 6 is CH 3 , R 11 is benzoyl or a substituted benzoyl (preferably R 11 is 4-chloro-benzoyl), X is F, and X" is Br.
  • the reagent is defined as above.
  • the reagent is at least one of Ph 3 P/CBr 4 , Ph 3 P/CHBr 3 ,
  • the stereoselective conversion can be performed in the presence of certain solvents, which include, but are not limited to, CH 2 Cl 2 , 1 ,2- dichloroethane, toluene, chlorobenzene, tetrahydrofuran, dioxane, diethyl ether, dimethylformamide, acetonitrile, N-methylpyrrolidine, dimethoxyethane, preferably the solvent used is CH 2 Cl 2 .
  • the stereoselective conversion can be performed at a temperature that ranges from about (-78 0 C) to about 0 0 C.
  • the temperature ranges from about (-78 0 C) to about (-10 0 C).
  • the temperature ranges from about (-30 0 C) to about (-10 0 C).
  • a fourth embodiment is directed to a compound IV.
  • R 5 , R 6 , and X have the meanings as defined herein above and R 11 is a protecting group.
  • R 5 is H
  • R 6 is CH 3
  • R 11 is a protecting group
  • R 11 is is benzoyl or a substituted benzoyl and more preferably R 11 is 4- chloro-benzoyl
  • X is F, preferably R 11 is 4-chloro-benzoyl.
  • the compound IV can be purified or unpurif ⁇ ed.
  • the compound IV is useful for preparing compound I and compound II.
  • a fifth embodiment is directed to a compound V.
  • R 5 , R 6 , R 11 , X, and X' have the meanings set forth above.
  • R 5 , R 6 , R 11 , X, and X' have the meanings set forth above.
  • R 5 is H, R 6 is CH 3 , R 11 is a protecting group, preferably R 11 is is benzoyl or a substituted benzoyl and more preferably R 11 is 4-chloro-benzoyl, X is F, and X' is Cl, Br, or I (most preferably X' is Br).
  • the compound V can be purified or unpurified. The compound V is useful for preparing a compound I and a compound II.
  • An sixth embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, comprising stereoselective coupling of an alpha- derivative V with a purine or a derivatized purine base using a basic reagent to produce a beta-nucleoside VI
  • R 5 , R 6 , R 9 X, and Z have the meanings as defined herein above
  • R 11 is a protecting group
  • X' and X" independent of each other are leaving groups.
  • a first aspect of the sixth embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, wherein for the compound V or the compound VI, R 5 is H, R 6 is CH3, R 9 is NH 2 , R 11 is a protecting group, preferably R 11 is is benzoyl or a substituted benzoyl and more preferably R 11 is 4-chloro- benzoyl, X is F, X' is Br, X" is Cl, and Z is N.
  • the basic reagent is as defined above.
  • the basic reagent comprises a (lower alk)oxide ((lower alkyl)OM) and an alcoholic solvent.
  • the basic reagent is MeONa/MeOH, EtONa/EtOH, or 'BUOK/BUOH. Most preferably, the basic reagent is 'BUOK/BUOH.
  • the stereoselective coupling can be performed in a solvent comprising at least one of a polar aprotic solvent, a non-polar solvent, and a polar solvent. Examples of polar aprotic solvents include, but are not limited to, acetonitrile, dimethylsulfoxide, N 5 N- dimethylformamide, hexamethylphosphoramide, etc.
  • the stereoselective coupling can be performed at a temperature that ranges from about 0 0 C up to about the reflux temperature of the solvent. Preferably, the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 6O 0 C. Most preferably, the temperature is about 50 0 C.
  • a seventh embodiment is directed to a compound VI.
  • R 5 , R 6 , R 9 X, and Z have the meanings as defined herein above
  • R 11 is a protecting group
  • X" is a leaving group.
  • R 11 is benzoyl or a substituted benzoyl, more preferably R 11 is 4-chloro-benzoyl
  • R 5 is H
  • R 6 is CH 3
  • X is F
  • X" is Cl
  • R 9 is NH 2
  • Z is N.
  • Compound VI can be purified or unpurified.
  • Compound VI is useful for preparing compound I and compound II.
  • a first aspect of the seventh embodiment is directed to a process for preparing compound VI, wherein said process comprises:
  • a second aspect of the seventh embodiment is directed to a process for preparing compound Vl, where R 5 is H, R 6 is CH3, R 9 is NH 2 , R 11 is a protecting group, preferably R 11 is benzoyl or a substituted benzoyl and more preferably R 11 is 4-chloro-benzoyl, X is F, X' is Br, X" is Cl, and Z is N.
  • the stereoselective reduction can be performed in a solvent comprising tetrahydrofuran (THF) or diethyl ether, preferably the solvent is THF at a temperature ranging from about (-78 0 C) to about 25°C, preferably at a temperature ranging from about (-78 0 C) to about 0 0 C, and most preferably at a temperature from about (-30 0 C) to about 0 0 C.
  • the hydride reducing agent is ('BuO ⁇ AlH, sodium (bis(2-methoxyethoxy)(2,2,2-trifluoro- ethoxy)aluminum hydride, or Red- Al (sodium bis(2-methoxyethoxy)aluminum hydride).
  • the stereoselective coupling can be performed in a solvent comprising at least one of a polar aprotic solvent, a non-polar solvent, and a polar solvent.
  • polar aprotic solvents include, but are not limited to, acetonitrile, dimethylsulfoxide, N,N-dimethylformamide, hexamethylphosphoramide, etc.
  • the stereoselective coupling can be performed at a temperature that ranges from about 0 0 C up to about the reflux temperature of the solvent. Preferably, the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the basic reagent is as defined above.
  • the basic reagent comprises a (lower alk)oxide ((lower alkyl)OM) and an alcoholic solvent.
  • the basic reagent is MeONa/MeOH, EtONa/EtOH, EtOK/EtOH, 'PrONa/'PrOH, 'PrOK/PrOH, or 'BUOK/BUOH.
  • the basic reagent is 'BUOK/BUOH.
  • R > 5 , r R> 6 , r R> 9 , X, and Z have the meanings as defined above, each of X' and X" independent of each other is a leaving group, and R 11 is a protecting group.
  • R 5 is H
  • R 6 is CH3
  • R 11 is benzoyl or a substituted benzoyl (preferably R 11 is 4-chloro-benzoyl)
  • X is F
  • X is Br
  • X" is Cl
  • Z is N
  • R 9 is NH 2 .
  • the hydride reducing agent is ('BuO ⁇ AlH, sodium (bis(2-methoxyethoxy)(2,2,2- trifluoro-ethoxy)aluminum hydride, or Red- Al (sodium bis(2- methoxyethoxy)aluminum hydride).
  • the stereoselective reduction can be performed in a solvent comprising tetrahydrofuran (THF) or diethyl ether, preferably the solvent is THF at a temperature ranging from about (-78 0 C) to about 25°C, preferably at a temperature ranging from about (-78 0 C) to about 0 0 C, and most preferably at a temperature from about (-30 0 C) to about 0 0 C.
  • the crystallization occurs by a process comprising adding to the mixture seed crystals of the beta-lactol derivative and then heating the mixture comprising the seed crystals of the beta- lactol derivative at a temperature that ranges from about 25°C to about 8O 0 C, preferably about 50 0 C with (about 0.2 mmHg) or without applied vacuum.
  • the stereoselective coupling can be performed in a solvent comprising at least one of a polar aprotic solvent, a non-polar solvent, and a polar solvent.
  • polar aprotic solvents include, but are not limited to, acetonitrile, dimethylsulfoxide, N 5 N- dimethylformamide, hexamethylphosphoramide, etc.
  • the stereoselective coupling can be performed at a temperature that ranges from about 0 0 C up to about the reflux temperature of the solvent.
  • the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 6O 0 C. Most preferably, the temperature is about 50 0 C.
  • the basic reagent is as defined above.
  • the basic reagent comprises a (lower alk)oxide ((lower alkyl)OM) and an alcoholic solvent.
  • the basic reagent is MeONa/MeOH, EtONa/EtOH, EtOK/EtOH, 'PrONa/'PrOH, 'PrOK/PrOH, or 'BUOK/BUOH.
  • the basic reagent is 'BUOK/BUOH.
  • An eight embodiment is directed to a process for preparing a compound I or a compound II or any of the compounds recited in any one of the aspects of the first and second embodiments, comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-substituted nucleoside VII;
  • R 5 , R 6 , R 8 , R 9 , X, Z have the meanings as defined herein above, R 11 is a protecting group, and X" is a leaving group.
  • a first aspect of the eighth embodiment is directed to a process for preparing a compound I or a compound II, wherein for the the compound VI, the compound VII, or the compound VIII, R 5 is H, R 6 is CH 3 , R 8 is any one -OMe, -OEt, -O'Pr, - N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl), and -OBn, R 9 is NH 2 , R 11 is a protecting group (preferably benzoyl or substituted benzoyl, more preferably 4-chlor-benzoyl), X is F, X" is Cl, and Z is N.
  • the nucleophilic reagent is as defined above.
  • the nucleophilic reagent is one that provides for R 8 which is any one -OMe, -OEt, - O'Pr, -N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl), and -OBn.
  • the reacting can be performed in at least one solvent comprising at least one of a polar aprotic solvent, a non-polar solvent, and a polar solvent.
  • polar solvents include, but are not limited to, methanol, ethanol, t-butanol, benzyl alcohol.
  • polar aprotic solvents include, but are not limited to, acetonitrile, dimethylsulfoxide, N 5 N- dimethylformamide, hexamethylphosphoramide, etc.
  • the reacting can be performed at a temperature that ranges from about 0 0 C up to about the reflux temperature of the at least one solvent. Preferably, the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a second aspect of the eight embodiment is directed to a process for preparing a compound I or a compound II, comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-substituted nucleoside VII;
  • the nucleophilic reagent is MeONa/MeOH.
  • the reacting can be performed in a solvent comprising methanol.
  • the reacting can be performed at a temperature that ranges from about 0 0 C up to about 65°C.
  • the temperature ranges from about 25°C to about 65°C or alternatively from a temperature above room temperature to the boiling point of the alcoholic solvent/reagent. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a third aspect of the eighth embodiment is directed to a process for preparing a compound I or a compound II, comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-substituted nucleoside VII;
  • the nucleophilic reagent is as defined above.
  • the nucleophilic reagent can be prepared in situ by reacting EtOH a base, such as potassium carbonate. The reacting can be performed in ethanol and at a temperature that ranges from about 0 0 C up to about 78°C.
  • a fourth aspect of the eighth embodiment is directed to a process for preparing a compound I or a compound II comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-substituted nucleoside VII;
  • the nucleophilic reagent is BnONa/BnOH, wherein BnONa is obtained by a process comprising reacting benzyl alcohol with sodium hydride in a N ,N- dimethylformamide solution at a temperature achieved using an ice bath (about O 0 C). The reacting can be performed in a solvent comprising benzyl alcohol. The reacting can be performed at a temperature that ranges from about O 0 C up to about 75°C.
  • the temperature ranges from about 25°C to about 65°C. More preferably, the temperature ranges from about 40 0 C to about 6O 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a fifth aspect of the eighth embodiment is directed to a process for preparing a compound I or a compound II comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-substituted nucleoside VII;
  • R is H
  • R 6 is CH 3
  • R 8 is -N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl)
  • R 9 is NH 2
  • R 11 is a protecting group (preferably benzoyl or substituted benzoyl)
  • X is F
  • X" is Cl
  • Z is N (see compound (8) below).
  • the nucleophilic reagent is azetidine/triethyl amine.
  • the reacting can be performed in a solvent comprising ethanol.
  • the reacting can be performed at a temperature that ranges from about O 0 C up to about 78°C.
  • the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a ninth embodiment is directed to a compound VIII.
  • R > 5 , n Ro , X ⁇ ⁇ 7-, f R) 8 , f R) 9 , and Z have the meanings as defined herein above.
  • R 5 is H
  • R 6 is CH 3
  • X is F
  • R 8 is a -O(lower alkyl), -O(lower cycloalkyl), -O(lower alkaryl), or nitrogen heterocycle
  • R 9 is NH 2
  • Z is N.
  • R 5 is H
  • R 6 is CH 3
  • X is F
  • R 8 is any one of OMe, OEt, O'Pr, OBn, and N(-CH 2 CH 2 CH 2 -) (azetidin-1-yl)
  • R 9 is NH 2
  • Z is N.
  • the compound VIII can be purified or unpurified.
  • the compound VIII is purified.
  • a preferred compound VIII is represented by one of compounds 7, 8, 9, and
  • the compound VIII is useful for preparing a compound I and a compound II.
  • a tenth embodiment of is directed to a process comprising converting a free purine nucleoside derivative VIII to compound I or converting the free purine nucleoside derivative to compound II.
  • Procedures for converting a free purine nucleoside derivative VIII to compound I are disclosed herein, as well as U.S. Patent Application No. 12/053,015, filed March 21, 2008 (see also WO 2008/121634).
  • Procedures for converting a free purine nucleoside derivative VIII to compound II are disclosed herein and U.S. Provisional Patent Application No. 61/060,683, filed June 11, 2008.
  • a first aspect of the tenth embodiment related to preparing compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with either a P(III)-reagent or a P(V)-reagent.
  • a second aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(III)-reagent to obtain a phosphite derivative of II, wherein the phosphite derivative of II comprises a mixture of isomers.
  • a third aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(III)-reagent in the presence of an activator reagent to obtain a phosphite derivative of II, wherein the phosphite derivative of II comprises a mixture of isomers.
  • a fourth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(III)-reagent in the presence of an activator reagent to obtain a phosphite derivative of II, wherein the phosphite derivative of II comprises a mixture of isomers; and equilibrating the phosphite derivative of II to provide an equilibrium mixture of phosphite isomer derivatives of II.
  • a fifth aspect of the tenth embodiment related to preparing the 3',5'-cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP[N('Pr) 2 ]2 in the presence of 4,5- dicyanoimidazole to obtain a phosphite derivative of II
  • the phosphite derivative of II comprises a mixture of isomers and R 5 , R 6 , R 7 , R 8 , R 9 , X and Z are defined herein; and equilibrating the phosphite derivative of II to obtain an equilibrium mixture of phosphite isomer derivatives of II.
  • a sixth aspect of the tenth embodiment is related to the fifth aspect further comprising oxidizing the equilibrium mixture of phosphite isomer derivatives of II to obtain the 3',5'-cyclic phosphate derivative II
  • a seventh aspect of the tenth embodiment related to preparing the 3',5'-cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP [NCPr) 2 J 2 in the presence of 4,5- dicyanoimidazole to obtain a phosphite derivative of II
  • phosphite derivative of II comprises a mixture of isomers, R 5 is H, R 6 is CH 3 , X is F, Z is N, and R 9 is NH 2 , while R 7 and R 8 are defined herein; and equilibrating the phosphite derivative of II to obtain an equilibrium mixture of phosphite isomer derivatives of II.
  • An eighth aspect of the tenth embodiment is related to the seventh aspect further comprising oxidizing the equilibrium mixture of phosphite isomer derivatives of II to obtain the 3',5'-cyclic phosphate derivative II
  • a ninth aspect of the tenth embodiment related to preparing the 3',5'-cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP[N( 2 Pr) 2 J 2 in the presence of 4,5- dicyanoimidazole to obtain a phosphite derivative of II
  • the phosphite derivative of II comprises a mixture of isomers and R 5 is H, R 6 is CH 3 , R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, or O'Pr, R 9 is NH 2 , X is F, and Z is N. and equilibrating the phosphite derivative of II to obtain an equilibrium mixture of phosphite isomer derivatives of II.
  • a tenth aspect of the tenth embodiment is related to the ninth aspect further comprising oxidizing the equilibrium mixture of phosphite isomer derivatives of II to obtain the 3',5'-cyclic phosphate derivative II
  • An eleventh aspect of the tenth embodiment related to preparing the 3',5'- cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a 'PrOP[N('Pr) 2 ]2 in the presence of 4,5- dicyanoimidazole to obtain a phosphite derivative of II
  • the phosphite derivative of II comprises a mixture of isomers and R 5 is H, R 6 is CH 3 , R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, or O'Pr, R 9 is NH 2 , X is F, and Z is N; and equilibrating the phosphite derivative of II to obtain an equilibrium mixture of phosphite isomer derivatives of II.
  • a twelfth aspect of the tenth embodiment is related to the eleventh aspect further comprising oxidizing the equilibrium mixture of phosphite isomer derivatives of II to obtain a disasteromeric mixture of the 3',5'-cyclic phosphate derivatives s shown below.
  • a thirteenth aspect of the tenth embodiment related to preparing the 3',5'- cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a 'PrOP[N('Pr) 2 ] 2 in the presence of 4,5- dicyanoimidazole to obtain a phosphite derivative of II
  • the phosphite derivative of II comprises a mixture of isomers and R 5 is H, R 6 is CH 3 , R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, or O'Pr, R 9 is NH 2 , X is F, and Z is N; and equilibrating the phosphite derivative of II in a solution at a temperature to obtain an equilibrium mixture of phosphite isomer derivatives of II.
  • the above- noted solution comprises a polar solvent, a non-polar solvent, and mixtures thereof.
  • polar solvents include, but are not limited to, water, dimethylsulfoxide, N,N-dimethylformamide, hexamethylphosphoramidate, acetonitrile, ethyl acetate, tetrahydrofuran, etc. and mixtures thereof.
  • non-polar solvents include, but are not limited to, hexane, heptane, benzene, toluene, methylene chloride, chloroform, etc., and mixtures thereof.
  • the temperature can range over the entire available temperature range for the solution, which entails a temperature range where the solution does not solidify due to freezing, the solution does not evaporate due to boiling, or the solution components do not decompose.
  • the solution temperature is determined experimentally based on the equilibrium position of the mixture of isomers of the phosphite derivative of II. For instance, if the cis isomer is desired, then the desirable temperature or temperature range is one where the mole (or molar) ratio of the c ⁇ -isomer to the trans-isomcr is at an acceptable maximum.
  • a fourteenth aspect of the tenth embodiment is related to the eleventh aspect further comprising optionally isolating the equilibrium mixture as a solid and contacting an oxidizing agent and the equilibrium mixture of phosphite isomer derivatives of II in a solution comprising an organic solvent to obtain the 3',5'-cyclic phosphate derivatives s shown below.
  • the mole ratio of oxidizing agent to the phosphite derivative II ranges from about 0.9 to about 1.5, preferably from about 0.9 to about 1.2, more preferably from about 0.9 to about 1.1, most preferably the mole ratio of oxidizing agent to the phosphite derivative II is about 1.
  • the solution comprises tetrahydrofuran ("THF"), and the oxidizing agent comprises iodine (I 2 ).
  • the solution comprises THF, pyridine (“pyr”), and water in at least about one-molar equivalent relative to the phosphite derivative of II.
  • the solution comprises about 60 v/v % to about 80 v/v % of THF and about 39 v/v % to about 17 v/v % of pyr, and about 1 v/v% to about 3 v/v % of water, with the proviso that the amount of water is at least about one -molar equivalent relative to the phosphite derivative of II.
  • the solution comprises about 65 v/v % to about 75 v/v % of THF and about 34 v/v % to about 22 v/v % of pyr, and about 1 v/v% to about 3 v/v % of water, with the proviso that the amount of water is at least about one-molar equivalent relative to the phosphite derivative of II.
  • the solution comprises about 70 v/v % of THF, about 28 v/v of pyr, and about 2 v/v % of water, with the proviso that the amount of water is at least about one-molar equivalent relative to the phosphite derivative of II.
  • a fifteenth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(V)-reagent.
  • a sixteenth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(V)-reagent and an amine reagent.
  • a seventeenth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a P(V)-reagent selected from among P(O)(Lv) 3 , R 7 OP(O)(Lv) 2 , R 7 OP(O)(LV)(N(CL 6 alkyl) 2 ), R 7 OP(O)[N(CL 6 alkyl) 2 ] 2 and an amine reagent.
  • a P(V)-reagent selected from among P(O)(Lv) 3 , R 7 OP(O)(Lv) 2 , R 7 OP(O)(LV)(N(CL 6 alkyl) 2 ), R 7 OP(O)[N(CL 6 alkyl) 2 ] 2 and an amine reagent.
  • An eighteenth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP(O)(Lv) 2 and an amine reagent.
  • a nineteenth aspect of the tenth embodiment related to preparing the compound II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP(O)(Lv) 2 and an amine reagent, wherein R 5 is H, R 6 is CH 3 , R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, or O'Pr, R 9 is NH 2 , X is F, and Z is N.
  • a twentieth aspect of the tenth embodiment related to preparing the 3', 5'- cyclic phosphate derivative II is directed to a process which comprises reacting the free purine nucleoside derivative VIII with a R 7 OP(O)(Lv) 2 and an amine reagent, wherein R 5 is H, R 6 is CH 3 , R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, O'Pr, R 9 is NH 2 , X is F, and Z is N, Lv is Cl, and the amine reagent comprises triethyl amine and N-methylimidazole.
  • the phosphite derivative of II wherein the phosphite derivative of II where R 5 , R 6 , R 7 , R 8 , R 9 , X, and Z are as defined herein.
  • the phosphite derivative of II has R 5 is H, R 6 is CH 3 , R 7 is lower alkyl or lower cycloalkyl, R 8 is a -O(lower alkyl), -O(lower cycloalkyl), or -OBn, R 9 is NH 2 , X is F, and Z is N.
  • the phosphite derivative of II has R 5 is H, R 6 is CH3, R 7 is Me, 'Pr, c Bu, or c Pn, R 8 is OMe, OEt, or O'Pr, R 9 is NH 2 , X is F, and Z is N.
  • An eleventh embodiment is directed to a process for preparing the comound I or the compound II, where for both R 8 is OH, said process comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ;
  • a first aspect of the eleventh embodiment is directed to a process for preparing a compound I, where R 8 is OH, said process comprising reacting the beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ;
  • R 11 is a protecting group (preferably benzoyl or 4-chloro-benzoyl, and more preferably 4-chloro-benzoyl)
  • X" is a leaving group
  • X"" is a group capable of being converted to OH; wherein the reacting can be performed in a solvent comprising ethanol and at a temperature that ranges from about O 0 C up to about 78°C.
  • the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a second aspect of the eleventh embodiment is directed to a process for preparing a compound I, where R 8 is OH, said process comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ;
  • R 11 is a protecting group (preferably benzoyl or 4-chloro-benzoyl, and more preferably 4-chloro-benzoyl)
  • X" is a leaving group
  • X"" is a group capable of being converted to OH; wherein the reacting can be performed in a solvent comprising ethanol and at a temperature that ranges from about O 0 C up to about 78°C. Preferably, the temperature ranges from about 25°C to about 75°C.
  • the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the group capable of being converted to OH (X'") is - OBn, an -O-silyl, or an -O-allyl. The deprotecting can occur as described herein.
  • a third aspect of the eleventh embodiment is directed to a process for preparing a compound I, where R 8 is OH, said process comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ; deprotecting the 6-X'"-substituted nucleoside VII 1 to produce a 6-X'"- substituted nucleoside IX;
  • R 1 is phenyl
  • R 2 is hydrogen
  • R 3a is hydrogen
  • R 3b is CH 3
  • R 4 is -lower alkyl or - lower cycloalkyl
  • R 5 is H
  • R 6 is CH 3
  • R 9 is NH 2
  • R 11 is a protecting group (preferably benzoyl or 4-chloro benzoyl)
  • X is F
  • X" is Cl
  • X" is -OBn, -O-silyl, or -O-allyl
  • Z is N; wherein the reacting can be performed in a solvent comprising ethanol and at a temperature that ranges from about 0 0 C up to about 78°C. Preferably, the temperature ranges from about 25°C to about 75°C.
  • the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the group capable of being converted to OH (X'") is - OBn, an -O-silyl, or an -O-allyl. The deprotecting can occur as described herein.
  • a fourth aspect of the eleventh embodiment is directed to a process for preparing a compound I, where R 8 is OH, said process comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ;
  • X'" is a group capable of being converted to OH; deprotecting the 6-X'"-substituted nucleoside VII 1 to produce a 6-X'"- substituted nucleoside IX;
  • R 1 is phenyl
  • R 2 is hydrogen
  • R 3a is hydrogen
  • R 3b is CH 3
  • R 4 is -lower alkyl or -lower cycloalkyl
  • R 5 is H
  • R 6 is CH 3
  • R 9 is NH 2
  • R 11 is a protecting group (preferably benzoyl or 4-chloro-benzoyl)
  • X is F
  • X" is Cl
  • X'" is -OBn or -O-allyl
  • Z is N; wherein the reacting can be performed in a solvent comprising ethanol and at a temperature that ranges from about 0 0 C up to about 78°C.
  • the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 6O 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a fifth aspect of the eleventh embodiment is directed to a process for preparing a compound I, where R 8 is OH, said process comprising reacting a beta-nucleoside derivative VI with a nucleophilic reagent to produce a 6-X'"-substituted nucleoside VII 1 ;
  • X'" is a group capable of being converted to OH; deprotecting the 6-X'"-substituted nucleoside VII 1 to produce a 6-X'"- substituted nucleoside IX;
  • R 1 is phenyl
  • R 2 is hydrogen
  • R 3a is hydrogen
  • R 3b is CH 3
  • R 4 is -Me, -Et, -"Pr, - Pr,
  • R 5 is H
  • R 6 is CH 3
  • R 9 is NH 2
  • R 11 is a protecting group (preferably benzoyl or substituted benzoyl)
  • X is F
  • X" is Cl
  • X'" is -OBn or -O-allyl
  • Z is N; wherein the reacting can be performed in a solvent comprising ethanol and at a temperature that ranges from about 0 0 C up to about 78°C.
  • the temperature ranges from about 25°C to about 75°C. More preferably, the temperature ranges from about 40 0 C to about 60 0 C. Most preferably, the temperature is about 50 0 C.
  • the deprotecting can occur as described herein.
  • a twelfth embodiment is directed to a process for preparing a compound I or compound II: said process comprising: ( a "l) stereoselective reduction of a protected ribonolactone III using a hydride reducing agent
  • X'" is a group capable of being converted to -OH.
  • Compounds prepared by the processes disclosed herein are useful for the treatment of any condition the result of an infection by any one of the following viral agents: hepatitis C virus, West Nile virus, yellow fever virus, degue virus, rhinovirus, polio virus, hepatitis A virus, bovine viral diarrhea virus and Japanese encephalitis virus.
  • the invention is related to a composition for the treatment and/or prophylaxis of any of the viral agents using compound I or II.
  • Possible viral agents include, but are not limited to: hepatitis C virus, hepatitus B virus, Hepatitis A virus, West Nile virus, yellow fever virus, dengue virus, rhinovirus, polio virus, bovine viral diarrhea virus, Japanese encephalitis virus, or those viruses belonging to the groups of Pestiviruses, hepaciviruses, or flavaviruses.
  • a preferred compound of formula I is represented by compound 11, while a preferred compound of formula II is represented by compound 17, more preferably i?p-17.
  • An aspect of this embodiment is directed to a composition for the treatment of any of the viral agents disclosed herein said composition comprising a pharmaceutically acceptable medium selected from among an excipient, carrier, diluent, and equivalent medium and compound I or II.
  • the compound I or II may be independently formulated in a wide variety of oral administration dosage forms and carriers. Oral administration can be in the form of tablets, coated tablets, hard and soft gelatin capsules, solutions, emulsions, syrups, or suspensions.
  • the compound I or II is efficacious when administered by suppository administration, among other routes of administration.
  • the most convenient manner of administration is generally oral using a convenient daily dosing regimen which can be adjusted according to the severity of the disease and the patient's response to the antiviral medication.
  • the compound I or II, as well as their pharmaceutically acceptable salts, together with one or more conventional excipients, carriers, or diluents, may be placed into the form of pharmaceutical compositions and unit dosages.
  • the pharmaceutical compositions and unit dosage forms may be comprised of conventional ingredients in conventional proportions, with or without additional active compounds and the unit dosage forms may contain any suitable effective amount of the active ingredient commensurate with the intended daily dosage range to be employed.
  • the pharmaceutical compositions may be employed as solids, such as tablets or filled capsules, semisolids, powders, sustained release formulations, or liquids such as suspensions, emulsions, or filled capsules for oral use; or in the form of suppositories for rectal or vaginal administration.
  • a typical preparation will contain from about 5% to about 95% active compound or compounds (w/w).
  • the compound I or II can be administered alone but will generally be administered in admixture with one or more suitable pharmaceutical excipients, diluents or carriers selected with regard to the intended route of administration and standard pharmaceutical practice.
  • a pharmaceutically acceptable salt form of an active ingredient may also initially confer a desirable pharmacokinetic property on the active ingredient which were absent in the non-salt form, and may even positively affect the pharmacodynamics of the active ingredient with respect to its therapeutic activity in the body.
  • Solid form preparations include, for example, powders, tablets, pills, capsules, suppositories, and dispersible granules.
  • a solid carrier may be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material.
  • the carrier In powders, the carrier generally is a finely divided solid which is a mixture with the finely divided active component.
  • the active component In tablets, the active component generally is mixed with the carrier having the necessary binding capacity in suitable proportions and compacted in the shape and size desired.
  • Suitable carriers include but are not limited to magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like.
  • Solid form preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
  • solid formulations are exemplified in EP 0524579; US 6,635,278; US 2007/0099902; US 7,060,294; US 2006/0188570; US 2007/0077295; US 2004/0224917; US 7,462,608; US 2006/0057196; US 6,267,985; US 6,294,192; US 6,569,463; US 6,923,988; US 2006/0034937; US 6,383,471; US 6,395,300; US 6,645,528; US 6,932,983; US 2002/0142050; US 2005/0048116; US 2005/0058710; US 2007/0026073; US 2007/0059360; and US 2008/0014228, each of which is incorporated by reference.
  • Liquid formulations also are suitable for oral administration include liquid formulation including emulsions, syrups, elixirs and aqueous suspensions. These include solid form preparations which are intended to be converted to liquid form preparations shortly before use. Examples of liquid formulation are exemplified in U.S. Patent Nos. 3,994,974; 5,695,784; and 6,977,257.
  • Emulsions may be prepared in solutions, for example, in aqueous propylene glycol solutions or may contain emulsifying agents such as lecithin, sorbitan monooleate, or acacia.
  • Aqueous suspensions can be prepared by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well known suspending agents.
  • the compound I or II may be independently formulated for administration as suppositories.
  • a low melting wax such as a mixture of fatty acid glycerides or cocoa butter is first melted and the active component is dispersed homogeneously, for example, by stirring. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool, and to solidify.
  • the compound I or II may be independently formulated for vaginal administration. Pessaries, tampons, creams, gels, pastes, foams or sprays containing in addition to the active ingredient such carriers as are known in the art to be appropriate. Certain of these formulations may also be used in conjunction with a condom with or without a spermicidal agent.
  • the purified compound I or II may be independently formulated in conjunction with liposomes or micelles.
  • liposomes it is contemplated that the purified compounds can be formulated in a manner as disclosed in U.S. Patent Nos. 5,013,556; U.S.
  • the purified compounds can be formulated in a manner as disclosed in U.S. Patent Nos. 5,145,684 and 5,091,188, both of which are incorporated by reference.
  • the fourteenth embodiment is directed to a use compound I or II in the manufacture of a medicament for the treatment of any condition the result of an infection by any one of the following viral agents: hepatitis C virus, West Nile virus, yellow fever virus, degue virus, rhinovirus, polio virus, hepatitis A virus, bovine viral diarrhea virus and Japanese encephalitis virus.
  • a preferred compound of formula I is represented by compound 11, while a preferred compound of formula II is represented by compound 17, more preferably i?p-17.
  • the term “medicament” means a substance used in a method of treatment and/or prophylaxis of a subject in need thereof, wherein the substance includes, but is not limited to, a composition, a formulation, a dosage form, and the like, comprising compound I or II. It is contemplated that the use of any of compound I or II in the manufacture of a medicament, for the treatment of any of the antiviral conditions disclosed herein, either alone or in combination with another compound.
  • a medicament includes, but is not limited to, any one of the compositions contemplated by the thirteenth embodiment.
  • a fifteenth embodiment is directed to a method of treatment and/or prophylaxis in a subject in need thereof said method comprises administering a therapeutically effective amount of any of compound I or II to the subject.
  • a preferred compound of formula I is represented by compound 11, while a preferred compound of formula II is represented by compound 17, more preferably R P -l7.
  • a subject in need thereof is one that has any condition the result of an infection by any of the viral agents disclosed herein, which includes, but is not limited to, hepatitis C virus, West Nile virus, yellow fever virus, degue virus, rhinovirus, polio virus, hepatitis A virus, bovine viral diarrhea virus or Japanese encephalitis virus, flaviviridae viruses or pestiviruses or hepaciviruses or a viral agent causing symptoms equivalent or comparable to any of the above-listed viruses.
  • the viral agents disclosed herein includes, but is not limited to, hepatitis C virus, West Nile virus, yellow fever virus, degue virus, rhinovirus, polio virus, hepatitis A virus, bovine viral diarrhea virus or Japanese encephalitis virus, flaviviridae viruses or pestiviruses or hepaciviruses or a viral agent causing symptoms equivalent or comparable to any of the above-listed viruses.
  • subject means a mammal, which includes, but is not limited to, cattle, pigs, sheep, chicken, turkey, buffalo, llama, ostrich, dogs, cats, and humans, preferably the subject is a human. It is contemplated that in the method of treating a subject thereof of the fifteenth embodiment can be any of the compounds contemplated herein, either alone or in combination with another compound.
  • terapéuticaally effective amount means an amount required to reduce symptoms of the disease in an individual.
  • the dose will be adjusted to the individual requirements in each particular case. That dosage can vary within wide limits depending upon numerous factors such as the severity of the disease to be treated, the age and general health condition of the patient, other medicaments with which the patient is being treated, the route and form of administration and the preferences and experience of the medical practitioner involved.
  • a daily dosage of between about 0.001 and about 10 g including all values in between, such as 0.001, 0.0025, 0.005, 0.0075, 0.01, 0.025, 0.050, 0.075, 0.1, 0.125, 0.150, 0.175, 0.2, 0.25, 0.5, 0.75, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, and 9.5, per day should be appropriate in monotherapy and/or in combination therapy.
  • a particular daily dosage is between about 0.01 and about 1 g per day, including all incremental values of 0.01 g (i.e., 10 mg) in between, a preferred daily dosage about 0.01 and about 0.8 g per day, more preferably about 0.01 and about 0.6 g per day, and most preferably about 0.01 and about 0.25 g per day, each of which including all incremental values of 0.01 g in between.
  • treatment is initiated with a large initial "loading dose" to rapidly reduce or eliminate the virus following by a decreasing the dose to a level sufficient to prevent resurgence of the infection.
  • Therapeutic efficacy can be ascertained from tests of liver function including, but not limited to protein levels such as serum proteins (e.g., albumin, clotting factors, alkaline phosphatase, aminotransferases (e.g., alanine transaminase, aspartate transaminase), 5 '-nucleosidase, ⁇ -glutaminyltranspeptidase, etc.), synthesis of bilirubin, synthesis of cholesterol, and synthesis of bile acids; a liver metabolic function, including, but not limited to, carbohydrate metabolism, amino acid and ammonia metabolism. Alternatively the therapeutic effectiveness may be monitored by measuring HCV-RNA. The results of these tests will allow the dose to be optimized.
  • serum proteins e.g., albumin, clotting factors, alkaline phosphatase, aminotransferases (e.g., alanine transaminase, aspartate transaminase), 5 '-nucle
  • a first aspect of the fifteenth embodiment is directed to a method of treatment and/or prophylaxis in a subject in need thereof said method comprises administering to the subject a therapeutically effective amount of a compound represented by any of compound I (preferably compound 11) or II (preferably compound 17, more preferably i?p-17) and a therapeutically effective amount of another antiviral agent; wherein the administration is concurrent or alternative.
  • the time between alternative administration can range between 1-24 hours, which includes any sub-range in between including, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, and 23 hours.
  • another antiviral agent examples include, but are not limited to: HCV NS3 protease inhibitors (see WO 2008010921, WO 2008010921, EP 1881001, WO 2007015824, WO 2007014925, WO 2007014926, WO 2007014921, WO 2007014920, WO 2007014922, US 2005267018, WO 2005095403, WO
  • HCV NS5B Inhibitors see US 2007275947, US20072759300, WO2007095269, WO 2007092000, WO 2007076034, WO 200702602, US 2005-98125, WO 2006093801, US 2006166964, WO 2006065590, WO 2006065335, US 2006040927, US 2006040890, WO 2006020082, WO
  • Concurrent administration as used herein thus includes administration of the agents at the same time or at different times. Administration of two or more agents at the same time can be achieved by a single formulation containing two or more active ingredients or by substantially simultaneous administration of two or more dosage forms with a single active agent.
  • references herein to treatment extend to prophylaxis as well as to the treatment of existing conditions.
  • treatment also includes treatment or prophylaxis of a disease or a condition associated with or mediated by HCV infection, or the clinical symptoms thereof.
  • An alternative common intermediate is the 4- chlorobenzoyl analog Ia, which can be produced either by debenzoylation of Ia to form the intermediate lactone diol, Ia', followed by 4-chlorobenzoylation, or by substituting 4-chlorobenzoyl chloride directly to react with Ia'.
  • An unexpected feature of the 3,5-di(4-chloro-benzoylated) intermediates is that they tend to have better crystalline properties, compared to the 3,5-di(benzoylated) intermediates, and so provides for an alternative means of purification in addition to chromatography.
  • the preferred approach became the Sj ⁇ 2 type reaction using a halo-sugar and a salt of the purine base. Again, the challenge of this approach was how to obtain an ⁇ halo-sugar stereospecifically in high yield to take advantage the inversion of configuration expected with Sj ⁇ 2 type reactions.
  • a typical method treats an anomeric mixture of the 1-O-acetate of a sugar with HCl or HBr in acetic acid. However, this method resulted in production of unfavorable anomeric mixtures.
  • the ⁇ -anomer (6a) could be selectively crystallized out from a methanolic solution to give the pure desired ⁇ - anomer (6a) in 55% yield from the bromosugar (4a).
  • conversion to unprotected 2-amino-6- substituted purines e.g., 7-10 was accomplished.
  • Further conversion to the phosphoramidate derivatives e.g., 11-14 proceeded by an adaptation of the method of Lehsten et al., Org. Proc. Res. Dev., 2002, 6, 819-822 or as disclosed in U.S. Patent Application No. 12/053,015, filed March 21, 2008, pp. 651-675.
  • Cyclic phosphate derivatives e.g., 15-17 were prepared as described in Can J. Chem.,
  • the 4-chlorobenzoyl lactone analog (Ib) can be produced either by debenzoylation of Ia to form the intermediate lactone diaol, Ia', followed by 4-chlorobenzoylation, or by substituting 4-chlorobenzoyl chloride directly to react with Ia'.
  • the reaction was quenched via addition of water (60 mL) and then the solution was concentrated under reduced pressure. The residue was diluted with ethyl acetate (200 mL)and washed with water, brine (2x100 mL each). The organic layer was concentrated under reduced pressure and the residue was purified by column chromatography (20% EtOAc in hexanes) to afford the product as a light yellow fluffy solid. The product was dried under (0.2 mmHg, 50 0 C, 2 h) to afford 24.3 g (46%), mp: 138-14FC.
  • the residue was purified by plug column (2.2 kg of 40-63 micron silica gel, packed in a 6 L sintered glass funnel, 22 cm length of silica gel, diameter 15 cm) using suction and a step-gradient of 5%, 10%, 20%, and 30% ethyl acetate in hexanes -ca 5 L of each).
  • the product containing fractions were combined and concentrated under reduced pressure to a colorless, very thick liquid (310.4 g).
  • the white solid thus collected (293.8 g, 77%) has a mp of 79-80 0 C and ratio of ⁇ / ⁇ is 20:1 based on NMR.
  • the reaction was judged to be >95% complete by TLC (RfS 0.61 ( ⁇ ), 0.72 ( ⁇ ), 0.36 lactol; 20% EtOAc in hexanes).
  • the reaction solution was immediately transferred to a vessel containing 230 g of flash chromatography grade silica gel (40-63 microns).
  • the stirred mixture was immediately passed through a pad of silica gel (680 g) in a 2.5 L sintered glass Buchner funnel.
  • the residue was purified by plug column chromatography using 2.1 kg of silica gel in a 6 L sintered glass Buchner funnel and eluted (via suction) with a stepwise gradient elution of 1%, 5%, 8% 12% EtOAc in hexane (ca 4 L each) to remove non-polar impurities followed by 12%, 25% EtOAc in hexane (6 L total) to elute the product.
  • the product containing fractions were combined into two fractions, concentrated under reduced pressure, dried under vacuum (0.1 mmHg, ambient temp., 20 h) to colorless oils.
  • the bromide solution was added to the purine base suspension over 1 min at ambient temperature.
  • the 5L flask was rinsed with acetonitrile (2x1 L) to transfer bromide completely to the reaction mixture.
  • the mixture was heated gradually to 50 0 C over 2 h with a heating mantle and controller, and stirred for 20 h.
  • the reaction was almost complete as shown by TLC beta (R/ 0.28, 30% EtOAc in hexanes).
  • the reaction was quenched by the addition of sat. NH 4 Cl (200 mL) to form a suspension.
  • the suspended solid 1 was removed by filtration through a 3 cm pad of Celite in a 2.5 L porcelain Buchner funnel. The solid was washed with toluene (3x100 mL).
  • the combined filtrate was neutralized by adding 6 N HCl solution until pH 7 (approx 220 mL).
  • the mixture was concentrated under reduced pressure. When the volume of mixture was reduced to about one-third volume, additional precipitated solid was removed by filtration in a similar manner.
  • the filtrate was further concentrated to a volume of about 800 mL.
  • the mixture was loaded in line with a silica gel cartridge and separated via column chromatography using a gradient of methanol in dichloromethane 0 to 15% MeOH.
  • the product eluted out at 12% methanol in dichloromethane.
  • the product containing fractions were combined, concentrated under reduced pressure and dried under vacuum (0.2 mmHg, 50 0 C, 24 h) to a white powder solid (4.45 g, 98% yield), mp 199-202 0 C.
  • the residue was mixed with 420 mL of 2 N HCl (420 mL) and dichloromethane (1 L). The organic layer was separated and the aqueous layer was extracted with additional dichloromethane (3x 500 mL). To the aqueous layer, ethyl acetate (1.4 L) was added and solid sodium carbonate (86 g) was added portion- wise to neutralize the aqueous layer to ca pH 8 (caution: foaming). After separation of the organic layer, the solid in aqueous layer was dissolved by adding more water (200 mL). The aqueous layer was extracted with ethyl acetate (3x 700 mL and 400 mL).
  • phenyl dichlorophosphate (2.66 g, 12.61 mmol) and anhydrous dichloromethane (40 mL).
  • the amino ester salt (2.6O g, 15.53 mmol) was added to the solution and the mixture was cooled to -5°C.
  • TV-Methyl imidazole (7.7 mL, 97 mmol) was then added quickly via a dry syringe at -5°C and the solution was stirred at -5°C for 1 h.
  • the nucleoside (7, 3.04 g, 9.7 mmol) was added from a vial in one portion at -5°C and the solid was slowly dissolved in 20 minutes.
  • reaction temperature was allowed to rise to ambient temperature over 2 h. After 17 h, the reaction was not complete. More chemical reagents were made (as described above from phosphate (2.66g), aminoester (2.6Og), and NMI (3.8 mL, 48 mmol)) and added to the reaction mixture at -5°C. The reaction was stirred at room temperature for 2 more hours. The reaction was almost complete as shown by TLC result and diluted with 70 mL of dichloromethane ⁇ Cl solution (1 N, 70 mL) was added. The aqueous layer was separated and extracted with dichloromethane. The organic layer was washed with saturated NaHCOs, water, brine and dried over MgSO 4 .
  • Example 14 using compounds (10) and (7a) as starting reagents.
  • phenyl dichlorophosphate (1.72 g, 8.15 mmol) and anhydrous dichloromethane (17 mL).
  • the amino ester (1.42 g, 10.2 mmol) was added and the suspension was cooled to - 5°C.
  • JV-Methylimidazole (3.34 g, 40.7 mmol) was added via a syringe in one portion and the solution was stirred at -5°C for 1 h under a nitrogen atmosphere.
  • the nucleoside (8, 1.38 g, 4.07 mmol) (foam solid) was then added in one portion and the solution was allowed to warm up over 1 h to ambient temperature.
  • phenyl dichlorophosphate (3.29 g, 15.58 mmol) and anhydrous dichloromethane (24 mL).
  • aminoester tosylate (white powder) was added and the solution was cooled to - 5°C under nitrogen.
  • N-Methylimidazole (4.92 g, 59.94 mmol) was added via a dry syringe in one portion and the resulted colorless clear solution was stirred at -5°C for one hour.
  • Example 22 N 6 ,N 6 -Diethyl-9-((4aR,6R,7R,7aR)-7-fluoro-2-methoxy-7-methyl- tetrahydro-furo[3,2-d] [l,3,2]dioxaphosphinin-6-yl)-9H-purine-2,6-diamine (phosphite precursor to 15)
  • the reaction was quenched upon the addition of water (0.1 mL).
  • the reaction solution was concentrated under reduced pressure and then the residue was triturated with ethyl acetate (5 mL).
  • the resulting white precipitate was removed by filtration and the filtrate was concentrated under reduced pressure.
  • the resulting intermediate cyclic phosphite residue was dissolved in acetonitrile (2 mL) and then treated with t-butyl hydroperoxide (70% in water, 0.19 mL) for 5 h at ambient temperature. TLC indicated a complete reaction.
  • the reaction solution was concentrated under reduced pressure and the residue was purified by column chromatography (Analogix using a gradient of 0 to 5% IPA in DCM). The two diastereomers were separable.
  • the P(III)-reagent cyclization reaction incorporating nucleoside 10 and a P(III)-reagent, such as 18 using lH-tetrazole as the activator initially gives an approximately equimolar mixture of cyclic phosphite diastereomers, but with elevated reaction temperature (50-60 0 C) for 6-24 hours, the mixture equilibrates predominantly to the cis isomer as reported in the literature.
  • 4,5-dicyanoimidazole, DCI accelerates this conversion rate and drives the equilibrium from initially approximately 80% to greater than 95% cis at 40-60 0 C after 6 h.
  • the c ⁇ -phosphite diastereomer leads to i?p-17 upon oxidation and also doubles the effective yield of this diastereomer and also simplifies the purification away from Sp-17.
  • the cyclic phosphate esters do not equilibrate under the reaction conditions.
  • Oxidation [ox] of the phosphite esters to the phosphate esters can be accomplished in many ways.
  • Peroxides such as m-chloroperbenzoic acid, t- butylhydroperoxide, hydrogen peroxide work well but are potentially explosive.
  • Urea hydrogen peroxide is a safer peroxide form that works well for this conversion.
  • the diastereomers, i?p-17and iSp-17 can be prepared directly from compound (10) by using an appropriate P(V)-reagent, as illustrated in Scheme 3.
  • Purification of i? P -17from iSp-17 and other by-products such as ring opened phosphates can be accomplished through washing an organic solution of the crude product with dilute base to remove free phosphates and the activating reagents followed by silica gel chromatography or crystallization or by a combination of both.
  • solvents include ethyl acetate, acetone and isopropanol.
  • an XPRD study of crystals from the three solvents showed a single polymorph which is the same as could be mathematically predicted from the single crystal x-ray from ethyl acetate.
  • the synthesis of the cyclic phosphate ester can be done either through P(III)- or P(V)-reagents. Chemistry involving the use of the P(III)-reagent requires an oxidation step for the intermediate cyclic phosphite esters as shown in Scheme 2.
  • the preferred route is to make the phosphorus reagent, isopropyl-N,N, N',N'-tetraisopropylphosphorodiamidite (18), which is not commercially available, but can be prepared readily by reacting commercially available chloro-N,N,N',N'-tetraisopropylphosphorodiamidite (19) with isopropanol in the presence of a tertiary amine base as described below.
  • Reagent 18 can be used as crude or it can be purified by vacuum distillation.
  • Example 27 6-Ethoxy-9-((2R,4aR,6R,7R,7aR)-7-fluoro-2-isopropoxy-7-methyl- 2-oxo-tetrahydro-2 ⁇ 5 -furo[3,2- ⁇ /] [1,3,2] dioxaphosphinin-6-yl)-9H-purin-2- ylamine (17-R P ) and 6-Ethoxy-9-((2S,4aR,6R,7R,7aR)-7-fluoro-2-isopropoxy-7- methyl-2-oxo-tetrahydro-2 ⁇ 5 -furo[3,2- ⁇ /][l,3,2]dioxaphosphinin-6-yl)-9H-purin- 2-ylamine (5 P -17)
  • the primary fraction was suspended in ethyl ether (250 mL) which immediately gave a fine granular solid.
  • the solid was collected by filtration and dried (40 0 C, 0.2mmHg, 17 h) to 73.5 g of slightly off white powder containing 20 mole% of ethyl ether.
  • the solid was co- evaporated with acetone (200 mL) and re-dried in a similar manner to 71.5 g of white solid with 2 mole% of acetone, and HPLC purity of 98.5%.
  • the secondary contaminated fractions were purified by chromatography to afford an additional 9.0 g for a total recovery of 80.5 g (41%) of pure product.
  • Portions of the desired Rp- 17solid could be re-crystallized into large irregular prisms by slow evaporation of product solutions in ethyl acetate, isopropanol and acetone.
  • a small portion of the lower isomer (Sp- 17 250 mg) was also isolated as an amorphous white foam solid.
  • Example 30 6-Methoxy-9-((2R,4aR,6R,7R,7aR)-7-fluoro-2-cyclobutoxy-7- methyl-2-oxo-tetrahydro-2 ⁇ s -furo[3,2-d][l,3,2]dioxaphosphinin-6-yl)-9H-purin- 2-ylamine (23)
  • the crude reaction mixture from a same scale reaction was diluted with dichloromethane (100 mL) and washed with 1 N HCl ( 2x100 mL) and water (2x50 mL). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure followed by high vacuum for 2 h to give 5.1 g of crude i?p-17.
  • One portion of the crude (750 mg) was dissolved in ethyl acetate (2,0 mL) and heated to reflux with stirring for 6 h. The resulting suspension was cooled to ambient temperature and stirred for 2Oh.
  • the maximum ⁇ / ⁇ in the final cycle of least squares was 0.009 and the two most prominent peaks in the
  • Table 1 lists cell information, data collection parameters, and refinement data. Final positional and equivalent isotropic thermal parameters are given in Table 2.
  • Figure 1 is an ORTEP representation of i?p-17 with 30% probability thermal ellipsoids displayed (ORTEP-II: A Fortran Thermal Ellipsoid Plot Program for Crystal Structure Illustrations". CK. Johnson (1976) ORNL-5138).
  • Samples run under ambient conditions were prepared as flat plate specimens using powder as received. Approximately 35 mg of the sample was gently packed into a cavity cut into polished, zero-background (510) silicon wafer. The sample was rotated in its own plane during analysis. The details of the data collection are: angular range: 2 to 42°2 ⁇ ; step size: 0.05°2 ⁇ ; and collection time: 4 s.step "1 .
  • X-Ray Powder Diffraction patterns were collected on a Bruker AXS C2 GADDS diffractometer using Cu Ka radiation (40 kV, 40 rnA), automated XYZ stage, laser video microscope for auto-sample positioning and a HiStar 2- dimensional area detector.
  • X-ray optics consists of a single G ⁇ bel multilayer mirror coupled with a pinhole collimator of 0.3 mm.
  • Figure 2 shows an XRD Diffractogram for i?p-17.
  • nucleoside 30 was dissolved in pyridine (30 mL) and CH 2 Cl 2 (100 mL). To the solution was added Ac 2 O (3 mL, excess) and the mixture was stirred at 0 0 C for 16 h. Water (10 mL) was added and the mixture was stirred at room temperature for 10 min. EtOAc (200 niL) was added and the solution was washed with water, brine and dried over Na 2 SO 4 .
  • HCV replicon assay HCV replicon assay.
  • HCV replicon RNA-containing Huh7 cells (clone A cells; Apath, LLC, St. Louis, Mo.) were kept at exponential growth in Dulbecco's modified Eagle's medium (high glucose) containing 10% fetal bovine serum, 4 mM L-glutamine and 1 mM sodium pyruvate, l ⁇ nonessential amino acids, and G418 (1,000 ⁇ g/ml).
  • Antiviral assays were performed in the same medium without G418. Cells were seeded in a 96-well plate at 1,500 cells per well, and test compounds were added immediately after seeding. Incubation time 4 days.
  • Replicon RNA and an internal control (TaqMan rRNA control reagents; Applied Biosystems) were amplified in a single-step multiplex RT-PCR protocol as recommended by the manufacturer.
  • the HCV primers and probe were designed with Primer Express software (Applied Biosystems) and covered highly conserved 5 '-untranslated region (UTR) sequences (sense, 5'-AGCCATGGCGTTAGTA(T)GAGTGT-3', and antisense, 5'-TTCCGCAGACCACTATGG-S'; probe, 5'-FAM- CCTCCAGGACCCCCCCTCCC-TAMRA-3').
  • the threshold RT-PCR cycle of the test compound was subtracted from the average threshold RT-PCR cycle of the no-drug control ( ⁇ Ct H cv)- A ⁇ Ct of 3.3 equals a 1-log 10 reduction (equal to the 90% effective concentration [EC90]) in replicon RNA levels.
  • the cytotoxicity of the test compound could also be expressed by calculating the ⁇ Ct rRN A values.
  • the ⁇ Ct specificity parameter could then be introduced ( ⁇ Ct H cv ⁇ ⁇ Ct r RNA), in which the levels of HCV RNA are normalized for the rRNA levels and calibrated against the no-drug control.

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TW201031675A (en) 2010-09-01
CN102325783A (zh) 2012-01-18
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AR074977A1 (es) 2011-03-02
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US9045520B2 (en) 2015-06-02
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AU2009329872B2 (en) 2016-07-07
KR20110104074A (ko) 2011-09-21

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