CN110964796B - 大规模平行单细胞分析 - Google Patents
大规模平行单细胞分析 Download PDFInfo
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- CN110964796B CN110964796B CN201911165393.0A CN201911165393A CN110964796B CN 110964796 B CN110964796 B CN 110964796B CN 201911165393 A CN201911165393 A CN 201911165393A CN 110964796 B CN110964796 B CN 110964796B
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Rigid containers without fluid transport within
- B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
- B01L3/50857—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
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- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/101—Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
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- Plant Pathology (AREA)
- Cell Biology (AREA)
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- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911165393.0A CN110964796B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361871232P | 2013-08-28 | 2013-08-28 | |
| US61/871,232 | 2013-08-28 | ||
| US201461952036P | 2014-03-12 | 2014-03-12 | |
| US61/952,036 | 2014-03-12 | ||
| US201462012237P | 2014-06-13 | 2014-06-13 | |
| US62/012,237 | 2014-06-13 | ||
| CN201480059505.3A CN105705659B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
| CN201911165393.0A CN110964796B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
| PCT/US2014/053301 WO2015031691A1 (en) | 2013-08-28 | 2014-08-28 | Massively parallel single cell analysis |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480059505.3A Division CN105705659B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110964796A CN110964796A (zh) | 2020-04-07 |
| CN110964796B true CN110964796B (zh) | 2024-04-05 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911165393.0A Active CN110964796B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
| CN201480059505.3A Active CN105705659B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480059505.3A Active CN105705659B (zh) | 2013-08-28 | 2014-08-28 | 大规模平行单细胞分析 |
Country Status (11)
| Country | Link |
|---|---|
| US (15) | US9567645B2 (https=) |
| EP (3) | EP3039158B1 (https=) |
| JP (2) | JP6545682B2 (https=) |
| KR (4) | KR102182834B1 (https=) |
| CN (2) | CN110964796B (https=) |
| AU (3) | AU2014312208B2 (https=) |
| DK (1) | DK3039158T3 (https=) |
| ES (2) | ES2711168T3 (https=) |
| GB (2) | GB2546833B (https=) |
| SG (2) | SG10201806890VA (https=) |
| WO (1) | WO2015031691A1 (https=) |
Families Citing this family (443)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060030036A1 (en) | 2004-05-28 | 2006-02-09 | Victor Joseph | Chips for multiplex analyses |
| KR101829182B1 (ko) | 2009-04-02 | 2018-03-29 | 플루이다임 코포레이션 | 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법 |
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| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
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| EP2820174B1 (en) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Methods and uses for molecular tags |
| WO2013134261A1 (en) | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
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| AU2013302867A1 (en) | 2012-08-13 | 2015-02-26 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) * | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA3216609C (en) | 2012-08-14 | 2024-05-14 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| WO2014093676A1 (en) | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| AU2014214682B2 (en) | 2013-02-08 | 2018-07-26 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| WO2014142850A1 (en) | 2013-03-13 | 2014-09-18 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
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| US20150298091A1 (en) * | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| SG11201609053YA (en) * | 2014-04-29 | 2016-11-29 | Illumina Inc | Multiplexed single cell gene expression analysis using template switch and tagmentation |
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| JP6593710B2 (ja) * | 2014-05-02 | 2019-10-23 | Idacセラノスティクス株式会社 | 単一細胞由来核酸の解析方法 |
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| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US10697007B2 (en) | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
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