ES2527426T3 - Detección combinada de agentes biológicos - Google Patents

Detección combinada de agentes biológicos Download PDF

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Publication number
ES2527426T3
ES2527426T3 ES05748001.4T ES05748001T ES2527426T3 ES 2527426 T3 ES2527426 T3 ES 2527426T3 ES 05748001 T ES05748001 T ES 05748001T ES 2527426 T3 ES2527426 T3 ES 2527426T3
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marker
probe
mixture
absence
detecting
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William A. Mcmillan
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Cepheid
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Cepheid
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un método para detectar al menos un primer y un segundo agentes biológicos, comprendiendo el primer agente un primer y un segundo marcadores, y comprendiendo el segundo agente un tercer y un cuarto marcadores, comprendiendo el método: (a) preparar una primera y una segunda mezclas a partir de al menos una y la misma muestra que se sospecha que contiene los agentes; (b) detectar la presencia o la ausencia del primer y del tercer marcadores en la primera mezcla en un primer recipiente; y (c) detectar la presencia o la ausencia del segundo y del cuarto marcadores en la segunda mezcla en un segundo recipiente; mediante lo cual la presencia del primer y del segundo marcadores indica la presencia del primer agente biológico en la muestra, y la presencia del tercer y del cuarto marcadores indica la presencia del segundo agente biológico en la muestra, en donde: la primera mezcla comprende una primera sonda que reconoce específicamente el primer marcador y una tercera sonda que reconoce específicamente el tercer marcador; la segunda mezcla comprende una segunda sonda que reconoce específicamente el segundo marcador y una cuarta sonda que reconoce específicamente el cuarto marcador; cada una de las sondas tiene un marcador detectable, el marcador detectable de la primera sonda es igual que el marcador detectable de la segunda sonda, y el marcador detectable de la tercera sonda es diferente del marcador detectable de la cuarta sonda; la etapa de detección (b) comprende determinar la presencia o la ausencia de la primera y de la tercera sondas que se unen al primer y al tercer marcadores en la primera mezcla; la etapa de detección (c) comprende determinar la presencia o la ausencia de la segunda y de la cuarta sondas que se unen al segundo y al cuarto marcadores en la segunda mezcla.

Description

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Claims (1)

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ES05748001.4T 2004-05-07 2005-05-06 Detección combinada de agentes biológicos Active ES2527426T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56920904P 2004-05-07 2004-05-07
US569209P 2004-05-07
PCT/US2005/016257 WO2005111243A2 (en) 2004-05-07 2005-05-06 Multiplexed detection of biological agents

Publications (1)

Publication Number Publication Date
ES2527426T3 true ES2527426T3 (es) 2015-01-23

Family

ID=35394752

Family Applications (1)

Application Number Title Priority Date Filing Date
ES05748001.4T Active ES2527426T3 (es) 2004-05-07 2005-05-06 Detección combinada de agentes biológicos

Country Status (5)

Country Link
US (1) US7294466B2 (es)
EP (1) EP1745155B1 (es)
JP (1) JP4951505B2 (es)
ES (1) ES2527426T3 (es)
WO (1) WO2005111243A2 (es)

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Also Published As

Publication number Publication date
JP2007535945A (ja) 2007-12-13
US20050250146A1 (en) 2005-11-10
JP4951505B2 (ja) 2012-06-13
WO2005111243A2 (en) 2005-11-24
EP1745155B1 (en) 2014-10-15
US7294466B2 (en) 2007-11-13
EP1745155A2 (en) 2007-01-24
WO2005111243A3 (en) 2007-03-01
EP1745155A4 (en) 2008-05-28

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