BRPI0620212B8 - método de discriminação e de contagem de pelo menos duas populações de elementos biológicos portadoras de características específicas, e, utilização do método - Google Patents
método de discriminação e de contagem de pelo menos duas populações de elementos biológicos portadoras de características específicas, e, utilização do métodoInfo
- Publication number
- BRPI0620212B8 BRPI0620212B8 BRPI0620212A BRPI0620212A BRPI0620212B8 BR PI0620212 B8 BRPI0620212 B8 BR PI0620212B8 BR PI0620212 A BRPI0620212 A BR PI0620212A BR PI0620212 A BRPI0620212 A BR PI0620212A BR PI0620212 B8 BRPI0620212 B8 BR PI0620212B8
- Authority
- BR
- Brazil
- Prior art keywords
- populations
- biological elements
- discrimination
- counting
- specific characteristics
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 abstract 3
- 238000000295 emission spectrum Methods 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 239000003550 marker Substances 0.000 abstract 1
- 238000001228 spectrum Methods 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1477—Multiparameters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/13—Tracers or tags
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Ecology (AREA)
- Dispersion Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
método de discriminação e de contagem de pelo menos duas populações de elementos biológicos portadoras de características específicas, e, utilização do método. a invenção tem como objeto um método de discriminação e de contagem de pelo menos duas populações de elementos biológicos portadoras de características específicas, eventualmente presentes em uma amostra. a invenção permite a detecção sem ambiquidade de pelo menos três populações de elementos biológicos pela utilização de somente dois meios de detecção, o que implica que pelo menos duas populações de elementos biológicos sejam detectadas por um único e mesmo meio de detecção. a invenção pode ser realizada se são utilizadas três sondas diferentes, cada uma delas reconhecendo e se fixando a uma das populações de elementos biológicos a detectar, cada uma das sondas sendo ela própria tornada detectável por um marcador diferente, dois dos ditos marcadores diferentes apresentado dois espectros de emissão que têm pelo menos uma parte comum (espectros de emissão superpostos) e o terceiro aparesentando um espectro de emissão que não tem essencialmente nenhuma parte comum com os dois outros (espectro não superposto).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0512948 | 2005-12-20 | ||
| FR0512948A FR2895087B1 (fr) | 2005-12-20 | 2005-12-20 | Methode de discrimination d'au moins deux populations cellulaires et application |
| PCT/FR2006/002645 WO2007080245A1 (fr) | 2005-12-20 | 2006-12-05 | Methode de discrimination d'au moins deux populations cellulaires et application |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI0620212A2 BRPI0620212A2 (pt) | 2013-01-15 |
| BRPI0620212B1 BRPI0620212B1 (pt) | 2018-02-14 |
| BRPI0620212B8 true BRPI0620212B8 (pt) | 2021-07-27 |
Family
ID=36259085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0620212A BRPI0620212B8 (pt) | 2005-12-20 | 2006-12-05 | método de discriminação e de contagem de pelo menos duas populações de elementos biológicos portadoras de características específicas, e, utilização do método |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US8080383B2 (pt) |
| EP (1) | EP1963859B1 (pt) |
| JP (1) | JP4782844B2 (pt) |
| KR (1) | KR20080082650A (pt) |
| CN (1) | CN101375164B (pt) |
| AT (1) | ATE490468T1 (pt) |
| BR (1) | BRPI0620212B8 (pt) |
| CA (1) | CA2632343A1 (pt) |
| DE (1) | DE602006018653D1 (pt) |
| ES (1) | ES2357035T3 (pt) |
| FR (1) | FR2895087B1 (pt) |
| RU (1) | RU2397494C2 (pt) |
| WO (1) | WO2007080245A1 (pt) |
| ZA (1) | ZA200804642B (pt) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2935802B1 (fr) * | 2008-09-05 | 2012-12-28 | Horiba Abx Sas | Procede et dispositif de classification, de visualisation et d'exploration de donnees biologiques |
| JP5452058B2 (ja) * | 2009-03-31 | 2014-03-26 | シスメックス株式会社 | 血液分析装置 |
| FR2956207B1 (fr) | 2010-02-10 | 2012-05-04 | Horiba Abx Sas | Dispositif et procede de mesures multiparametriques de microparticules dans un fluide |
| US20120173296A1 (en) * | 2011-01-03 | 2012-07-05 | Mcmullin Dale Robert | Method and system for outage restoration |
| RU2513511C1 (ru) * | 2012-12-19 | 2014-04-20 | Федеральное государственное бюджетное учреждение "Российский онкологический научный центр имени Н.Н. Блохина" Российской академии медицинских наук (ФГБУ "РОНЦ им. Н.Н. Блохина" РАМН) | Способ количественного определения клеток-предшественников (cd34+) в кроветворной ткани |
| US20140349313A1 (en) * | 2013-04-30 | 2014-11-27 | Becton, Dickinson And Company | Data analysis methods utilizing phenotypic properties |
| CN104792751B (zh) * | 2015-04-02 | 2017-06-23 | 清华大学 | 通过荧光共振能量转移方法原位定量活细胞中的蛋白质‑蛋白质相互作用 |
| CN108732082A (zh) * | 2018-06-07 | 2018-11-02 | 北京唯公医疗技术有限公司 | 细胞分群方法 |
| US11428692B2 (en) | 2018-07-11 | 2022-08-30 | Visiongate, Inc. | Specimen enrichment for optical tomography cell analysis |
| US10753875B1 (en) * | 2019-01-31 | 2020-08-25 | Rarecyte, Inc. | Spectral unmixing of spectroscopic emission images |
| CN110231275B (zh) * | 2019-05-20 | 2021-05-28 | 西安交通大学 | 一种基于流式细胞术检测nk细胞杀伤活性的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08510051A (ja) * | 1993-01-08 | 1996-10-22 | クールター コーポレイション | 保存対照細胞を蛍光および光散乱測定の校正において用いる方法 |
| GR940100272A (en) * | 1993-06-08 | 1995-11-29 | Ortho Diagnostic Systems Inc | Three-colour flow cytometry with automatic gating function. |
| US6900023B1 (en) * | 1999-09-02 | 2005-05-31 | Sysmex Corporation | Method for classifying and counting leukocytes |
| WO2003016866A2 (en) * | 2001-08-16 | 2003-02-27 | Litron Laboratories Ltd | Method for the enumeration of mammalian micronucleated erythrocyte populations with a single-laser flow cytometer |
| US7625712B2 (en) * | 2004-05-21 | 2009-12-01 | Beckman Coulter, Inc. | Method for a fully automated monoclonal antibody-based extended differential |
-
2005
- 2005-12-20 FR FR0512948A patent/FR2895087B1/fr not_active Expired - Fee Related
-
2006
- 2006-12-05 EP EP06841854A patent/EP1963859B1/fr not_active Not-in-force
- 2006-12-05 US US12/158,597 patent/US8080383B2/en not_active Expired - Fee Related
- 2006-12-05 KR KR1020087014810A patent/KR20080082650A/ko not_active Application Discontinuation
- 2006-12-05 AT AT06841854T patent/ATE490468T1/de not_active IP Right Cessation
- 2006-12-05 DE DE602006018653T patent/DE602006018653D1/de active Active
- 2006-12-05 JP JP2008546504A patent/JP4782844B2/ja not_active Expired - Fee Related
- 2006-12-05 CA CA002632343A patent/CA2632343A1/fr not_active Abandoned
- 2006-12-05 WO PCT/FR2006/002645 patent/WO2007080245A1/fr active Application Filing
- 2006-12-05 CN CN200680052947.0A patent/CN101375164B/zh not_active Expired - Fee Related
- 2006-12-05 BR BRPI0620212A patent/BRPI0620212B8/pt not_active IP Right Cessation
- 2006-12-05 ES ES06841854T patent/ES2357035T3/es active Active
- 2006-12-05 RU RU2008129429/15A patent/RU2397494C2/ru active
-
2008
- 2008-05-28 ZA ZA200804642A patent/ZA200804642B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RU2008129429A (ru) | 2010-01-27 |
| DE602006018653D1 (de) | 2011-01-13 |
| EP1963859A1 (fr) | 2008-09-03 |
| KR20080082650A (ko) | 2008-09-11 |
| ES2357035T3 (es) | 2011-04-15 |
| BRPI0620212A2 (pt) | 2013-01-15 |
| ATE490468T1 (de) | 2010-12-15 |
| US8080383B2 (en) | 2011-12-20 |
| FR2895087A1 (fr) | 2007-06-22 |
| WO2007080245A1 (fr) | 2007-07-19 |
| US20090311678A1 (en) | 2009-12-17 |
| JP4782844B2 (ja) | 2011-09-28 |
| EP1963859B1 (fr) | 2010-12-01 |
| CA2632343A1 (fr) | 2007-07-19 |
| ZA200804642B (en) | 2009-04-29 |
| CN101375164A (zh) | 2009-02-25 |
| FR2895087B1 (fr) | 2008-02-08 |
| BRPI0620212B1 (pt) | 2018-02-14 |
| JP2009520198A (ja) | 2009-05-21 |
| CN101375164B (zh) | 2014-01-01 |
| RU2397494C2 (ru) | 2010-08-20 |
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