EP1861512A4 - Thermal reaction device and method for using the same - Google Patents

Thermal reaction device and method for using the same

Info

Publication number
EP1861512A4
EP1861512A4 EP06739049A EP06739049A EP1861512A4 EP 1861512 A4 EP1861512 A4 EP 1861512A4 EP 06739049 A EP06739049 A EP 06739049A EP 06739049 A EP06739049 A EP 06739049A EP 1861512 A4 EP1861512 A4 EP 1861512A4
Authority
EP
European Patent Office
Prior art keywords
same
method
reaction device
thermal reaction
thermal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06739049A
Other languages
German (de)
French (fr)
Other versions
EP1861512A1 (en
Inventor
Marc A Unger
Lincoln Mcbride
Geoffrey Facer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fluidigm Corp
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US11/084,357 priority Critical patent/US7604965B2/en
Priority to US74996305P priority
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Priority to PCT/US2006/010103 priority patent/WO2006102264A1/en
Publication of EP1861512A1 publication Critical patent/EP1861512A1/en
Publication of EP1861512A4 publication Critical patent/EP1861512A4/en
Application status is Withdrawn legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0684Venting, avoiding backpressure, avoid gas bubbles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/142Preventing evaporation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/143Quality control, feedback systems
    • B01L2200/147Employing temperature sensors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0819Microarrays; Biochips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/12Specific details about materials
    • B01L2300/123Flexible; Elastomeric
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1805Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts
    • B01L2400/0655Valves, specific forms thereof with moving parts pinch valves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
EP06739049A 2003-04-03 2006-03-20 Thermal reaction device and method for using the same Withdrawn EP1861512A4 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/084,357 US7604965B2 (en) 2003-04-03 2005-03-18 Thermal reaction device and method for using the same
US74996305P true 2005-12-12 2005-12-12
PCT/US2006/010103 WO2006102264A1 (en) 2005-03-18 2006-03-20 Thermal reaction device and method for using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10197437A EP2371453A1 (en) 2005-03-18 2006-03-20 Microfluidic device
EP20120160084 EP2518161A1 (en) 2005-03-18 2006-03-20 Method for detection of mutant alleles

Publications (2)

Publication Number Publication Date
EP1861512A1 EP1861512A1 (en) 2007-12-05
EP1861512A4 true EP1861512A4 (en) 2009-12-09

Family

ID=37024154

Family Applications (3)

Application Number Title Priority Date Filing Date
EP20120160084 Ceased EP2518161A1 (en) 2003-04-03 2006-03-20 Method for detection of mutant alleles
EP06739049A Withdrawn EP1861512A4 (en) 2003-04-03 2006-03-20 Thermal reaction device and method for using the same
EP10197437A Withdrawn EP2371453A1 (en) 2003-04-03 2006-03-20 Microfluidic device

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP20120160084 Ceased EP2518161A1 (en) 2003-04-03 2006-03-20 Method for detection of mutant alleles

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP10197437A Withdrawn EP2371453A1 (en) 2003-04-03 2006-03-20 Microfluidic device

Country Status (2)

Country Link
EP (3) EP2518161A1 (en)
WO (1) WO2006102264A1 (en)

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EP1758981A4 (en) 2004-05-28 2013-01-16 Wafergen Inc Apparatus and methods for multiplex analyses
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
CA2677833C (en) * 2007-01-22 2016-05-03 Wafergen, Inc. Apparatus for high throughput chemical reactions
WO2008121691A1 (en) * 2007-03-28 2008-10-09 University Of Florida Research Foundation, Inc. Microfluidic array device and system for simultaneous detection of multiple analytes
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP5992911B2 (en) 2010-09-21 2016-09-14 ポピュレーション ジェネティクス テクノロジーズ リミテッド Increasing the reliability of allelic calls by molecular counting
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
EP3470533A1 (en) 2012-09-04 2019-04-17 Guardant Health, Inc. Systems and methods to detect copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
SG10201806890VA (en) 2013-08-28 2018-09-27 Cellular Res Inc Massively parallel single cell analysis
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ, インコーポレイテッド Method and system for digitally counting features on an array
SG10201804519RA (en) 2013-12-28 2018-07-30 Guardant Health Inc Methods and systems for detecting genetic variants
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN105653102B (en) * 2016-03-23 2018-12-21 南京铁道职业技术学院 Infrared induction electronic whiteboard
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
BR112019005900A2 (en) 2016-09-26 2019-06-11 Becton Dickinson Co protein expression measurement using reagents with barcode oligonucleotide sequences

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US20050252773A1 (en) * 2003-04-03 2005-11-17 Fluidigm Corporation Thermal reaction device and method for using the same

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Also Published As

Publication number Publication date
EP1861512A1 (en) 2007-12-05
WO2006102264A1 (en) 2006-09-28
EP2371453A1 (en) 2011-10-05
EP2518161A1 (en) 2012-10-31

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