ATE344834T1 - Methode zur herstellung von genormten und/oder subtrahierten cdna - Google Patents

Methode zur herstellung von genormten und/oder subtrahierten cdna

Info

Publication number
ATE344834T1
ATE344834T1 AT01120108T AT01120108T ATE344834T1 AT E344834 T1 ATE344834 T1 AT E344834T1 AT 01120108 T AT01120108 T AT 01120108T AT 01120108 T AT01120108 T AT 01120108T AT E344834 T1 ATE344834 T1 AT E344834T1
Authority
AT
Austria
Prior art keywords
cdna
tester
subtracted
normalized
preparing
Prior art date
Application number
AT01120108T
Other languages
English (en)
Inventor
Yoshihide Hayashizaki
Original Assignee
Riken
Dnaform Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken, Dnaform Kk filed Critical Riken
Application granted granted Critical
Publication of ATE344834T1 publication Critical patent/ATE344834T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paper (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT01120108T 2000-08-25 2001-08-22 Methode zur herstellung von genormten und/oder subtrahierten cdna ATE344834T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000255402 2000-08-25

Publications (1)

Publication Number Publication Date
ATE344834T1 true ATE344834T1 (de) 2006-11-15

Family

ID=18744193

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01120108T ATE344834T1 (de) 2000-08-25 2001-08-22 Methode zur herstellung von genormten und/oder subtrahierten cdna

Country Status (6)

Country Link
US (1) US7049065B2 (de)
EP (1) EP1197552B1 (de)
AT (1) ATE344834T1 (de)
CA (1) CA2356123A1 (de)
DE (1) DE60124363T2 (de)
HK (1) HK1045540A1 (de)

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DE60220025T2 (de) 2001-03-09 2008-01-17 Nugen Technologies, Inc., San Carlos Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2003106672A2 (en) * 2002-06-12 2003-12-24 Riken METHOD FOR UTILIZING THE 5'END OF mRNA FOR CLONING AND ANALYSIS
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
EP3167961A1 (de) * 2004-04-08 2017-05-17 Biomatrica, Inc. Integration von probenlagerung und probenverwaltung für biowissenschaften
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP1854882A4 (de) * 2005-03-01 2010-09-22 Wako Pure Chem Ind Ltd Verfahren zur gewinnung eines subtraktionspolynukleotids
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2806734A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (de) 2010-07-26 2017-03-15 Biomatrica, INC. Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen
GB201102385D0 (en) 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
US9322014B1 (en) 2012-11-28 2016-04-26 Sandia Corporation Multiplexed microfluidic approach for nucleic acid enrichment
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
EP3154338B1 (de) 2014-06-10 2020-01-29 Biomatrica, INC. Stabilisierung von thrombozyten bei umgebungstemperatur
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
CN108700498B (zh) 2015-12-08 2021-07-13 生物马特里卡公司 降低红细胞沉降速率
JP6417603B2 (ja) 2016-01-27 2018-11-07 株式会社ダナフォーム Ｒｎａの末端領域に対応する核酸の塩基配列を解読する方法およびｄｎａエレメントの分析方法
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
EP3788170A1 (de) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
CN112805389A (zh) 2018-10-01 2021-05-14 贝克顿迪金森公司 确定5’转录物序列
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
CN113574178A (zh) 2019-01-23 2021-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
CN114051534A (zh) 2019-07-22 2022-02-15 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4055160B1 (de) 2019-11-08 2024-04-10 Becton Dickinson and Company Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung
EP4090763A1 (de) 2020-01-13 2022-11-23 Becton Dickinson and Company Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
GB2621159A (en) * 2022-08-04 2024-02-07 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor

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EP1130116A3 (de) * 1992-04-06 2004-01-14 Abbott Laboratories Methode und Gerät zur Detektion von Nukleinsäuren unter Verwendung von totaler interner Reflektion
AU4745896A (en) * 1995-01-09 1996-07-31 Ambion, Inc. Methods and compositions for use in cleavage and detection of base pair mismatches in double-stranded nucleic acids
EP0839214A1 (de) * 1996-05-14 1998-05-06 Roche Diagnostics GmbH Verfahren zur identifizierung und/oder quantifizierung der expression von nukleinsäuremolekülen in einer probe
US5846721A (en) * 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries
US5741772A (en) * 1997-02-03 1998-04-21 Amgen Inc. Neurotrophic factor NNT-1
US5955594A (en) * 1997-04-30 1999-09-21 Mishra; Lopa Nucleic acids encoding proteins for early liver development
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof

Also Published As

Publication number Publication date
US7049065B2 (en) 2006-05-23
DE60124363T2 (de) 2007-09-06
US20020106666A1 (en) 2002-08-08
EP1197552B1 (de) 2006-11-08
EP1197552A3 (de) 2003-07-30
CA2356123A1 (en) 2002-02-25
HK1045540A1 (zh) 2002-11-29
DE60124363D1 (de) 2006-12-21
EP1197552A2 (de) 2002-04-17

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