DE602004029326D1 - Enzymatische Herstellung von Nukleinsäuremolekülen - Google Patents

Enzymatische Herstellung von Nukleinsäuremolekülen

Info

Publication number
DE602004029326D1
DE602004029326D1 DE602004029326T DE602004029326T DE602004029326D1 DE 602004029326 D1 DE602004029326 D1 DE 602004029326D1 DE 602004029326 T DE602004029326 T DE 602004029326T DE 602004029326 T DE602004029326 T DE 602004029326T DE 602004029326 D1 DE602004029326 D1 DE 602004029326D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
stranded
partially double
overhang
stranded overhang
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004029326T
Other languages
English (en)
Inventor
Octavian Dr Schatz
Heinz Dr Schwer
Gudrun Dr Horn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sloning Biotechnology GmbH
Original Assignee
Sloning Biotechnology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloning Biotechnology GmbH filed Critical Sloning Biotechnology GmbH
Publication of DE602004029326D1 publication Critical patent/DE602004029326D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE602004029326T 2004-01-23 2004-01-23 Enzymatische Herstellung von Nukleinsäuremolekülen Expired - Lifetime DE602004029326D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04001462A EP1557464B1 (de) 2004-01-23 2004-01-23 Enzymatische Herstellung von Nukleinsäuremolekülen

Publications (1)

Publication Number Publication Date
DE602004029326D1 true DE602004029326D1 (de) 2010-11-11

Family

ID=34626497

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004029326T Expired - Lifetime DE602004029326D1 (de) 2004-01-23 2004-01-23 Enzymatische Herstellung von Nukleinsäuremolekülen

Country Status (7)

Country Link
US (1) US8092991B2 (de)
EP (1) EP1557464B1 (de)
AT (1) ATE483020T1 (de)
DE (1) DE602004029326D1 (de)
DK (1) DK1557464T3 (de)
ES (1) ES2353302T3 (de)
WO (1) WO2005071077A1 (de)

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US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
RU2010101135A (ru) 2010-01-15 2011-07-20 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Бактерия семейства enterobacteriaceae - продуцент l-аспартата или метаболитов, производных l-аспартата, и способ получения l-аспартата или метаболитов, производных l-аспартата
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US9187777B2 (en) * 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP6118725B2 (ja) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． 核酸合成のための方法およびデバイス
RU2502803C2 (ru) * 2011-05-20 2013-12-27 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДА ДЛЯ ЭКСПРЕССИИ В КЛЕТКАХ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, НЕАКТИВНОГО ПРЕДШЕСТВЕННИКА ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНОВ, БАКТЕРИЯ, ПРИНАДЛЕЖАЩАЯ К РОДУ Escherichia, - ПРОДУЦЕНТ НЕАКТИВНОГО ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, ПРЕДШЕСТВЕННИК РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, СПОСОБ ПОЛУЧЕНИЯ КОНЪЮГАТОВ ПОЛИЭТИЛЕНГЛИКОЛЯ И РЕКОМБИНАНТНОГО МУТЕИНА ДНКазы I ЧЕЛОВЕКА, ФЕРМЕНТАТИВНО АКТИВНЫЙ КОНЪЮГАТ МУТЕИНА РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА
RU2495933C2 (ru) * 2011-05-30 2013-10-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДА ДЛЯ ЭКСПРЕССИИ В КЛЕТКАХ БАКТЕРИИ РОДА Escherichia ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА (ТАП) ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), БАКТЕРИЯ, ПРИНАДЛЕЖАЩАЯ К РОДУ Escherichia, - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ) ИЛИ [-1]МЕТИОНИЛ-ФРАГМЕНТ ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), ПРЕДШЕСТВЕННИК РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), СПОСОБ ПОЛУЧЕНИЯ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ)
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
DE102012101347B4 (de) * 2012-02-20 2014-01-16 Markus Fuhrmann Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
WO2015176070A1 (en) * 2014-05-16 2015-11-19 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
EP4253549A3 (de) * 2018-09-19 2023-12-06 The University of Hong Kong Verbessertes kombinatorisches genetisches modifikationssystem mit hohem durchsatz und optimierte cas9-enzymvarianten
US11873484B2 (en) 2019-11-27 2024-01-16 Microsoft Technology Licensing, Llc Oligonucleotide assembly using electrically controlled hybridization
US20210205775A1 (en) * 2020-01-06 2021-07-08 Microsoft Technology Licensing, Llc Solid-phase polymer synthesis on reusable substrates

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US5273879A (en) 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
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US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5770365A (en) 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
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US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
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Also Published As

Publication number Publication date
EP1557464B1 (de) 2010-09-29
US8092991B2 (en) 2012-01-10
EP1557464A1 (de) 2005-07-27
US20090298133A1 (en) 2009-12-03
DK1557464T3 (da) 2011-01-24
WO2005071077A1 (en) 2005-08-04
ATE483020T1 (de) 2010-10-15
ES2353302T3 (es) 2011-03-01

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