ATE483020T1 - Enzymatische herstellung von nukleinsäuremolekülen - Google Patents

Enzymatische herstellung von nukleinsäuremolekülen

Info

Publication number
ATE483020T1
ATE483020T1 AT04001462T AT04001462T ATE483020T1 AT E483020 T1 ATE483020 T1 AT E483020T1 AT 04001462 T AT04001462 T AT 04001462T AT 04001462 T AT04001462 T AT 04001462T AT E483020 T1 ATE483020 T1 AT E483020T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
stranded
partially double
overhang
stranded overhang
Prior art date
Application number
AT04001462T
Other languages
English (en)
Inventor
Octavian Schatz
Heinz Schwer
Gudrun Horn
Original Assignee
Sloning Biotechnology Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloning Biotechnology Gmbh filed Critical Sloning Biotechnology Gmbh
Application granted granted Critical
Publication of ATE483020T1 publication Critical patent/ATE483020T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04001462T 2004-01-23 2004-01-23 Enzymatische herstellung von nukleinsäuremolekülen ATE483020T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04001462A EP1557464B1 (de) 2004-01-23 2004-01-23 Enzymatische Herstellung von Nukleinsäuremolekülen

Publications (1)

Publication Number Publication Date
ATE483020T1 true ATE483020T1 (de) 2010-10-15

Family

ID=34626497

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04001462T ATE483020T1 (de) 2004-01-23 2004-01-23 Enzymatische herstellung von nukleinsäuremolekülen

Country Status (7)

Country Link
US (1) US8092991B2 (de)
EP (1) EP1557464B1 (de)
AT (1) ATE483020T1 (de)
DE (1) DE602004029326D1 (de)
DK (1) DK1557464T3 (de)
ES (1) ES2353302T3 (de)
WO (1) WO2005071077A1 (de)

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US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US9187777B2 (en) * 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
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RU2495933C2 (ru) * 2011-05-30 2013-10-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДА ДЛЯ ЭКСПРЕССИИ В КЛЕТКАХ БАКТЕРИИ РОДА Escherichia ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА (ТАП) ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), БАКТЕРИЯ, ПРИНАДЛЕЖАЩАЯ К РОДУ Escherichia, - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ) ИЛИ [-1]МЕТИОНИЛ-ФРАГМЕНТ ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), ПРЕДШЕСТВЕННИК РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ), СПОСОБ ПОЛУЧЕНИЯ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО ФРАГМЕНТА ТАП ЧЕЛОВЕКА (РЕТЕПЛАЗЫ)
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
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DE102012101347B4 (de) 2012-02-20 2014-01-16 Markus Fuhrmann Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
JP6509727B2 (ja) 2012-06-25 2019-05-15 ギンゴー バイオワークス， インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
WO2015176070A1 (en) * 2014-05-16 2015-11-19 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
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JP2022501025A (ja) * 2018-09-19 2022-01-06 ザ・ユニバーシティ・オブ・ホンコンThe University Of Hong Kong 改良されたハイスループットコンビナトリアル遺伝子改変システムおよび最適化されたＣａｓ９酵素変異体
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Also Published As

Publication number Publication date
EP1557464A1 (de) 2005-07-27
EP1557464B1 (de) 2010-09-29
US8092991B2 (en) 2012-01-10
WO2005071077A1 (en) 2005-08-04
ES2353302T3 (es) 2011-03-01
DE602004029326D1 (de) 2010-11-11
US20090298133A1 (en) 2009-12-03
DK1557464T3 (da) 2011-01-24

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