ATE538200T2 - Verfahren zur herstellung einer nukleinsäurenbibliothek - Google Patents

Verfahren zur herstellung einer nukleinsäurenbibliothek Download PDF

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Publication number
ATE538200T2
ATE538200T2 AT09004736T AT09004736T ATE538200T2 AT E538200 T2 ATE538200 T2 AT E538200T2 AT 09004736 T AT09004736 T AT 09004736T AT 09004736 T AT09004736 T AT 09004736T AT E538200 T2 ATE538200 T2 AT E538200T2
Authority
AT
Austria
Prior art keywords
stranded
oligonucleotide
nucleotides
stretch
library
Prior art date
Application number
AT09004736T
Other languages
English (en)
Inventor
Den Brulle Jan Van
Markus Fuhrmann
Ralf Strohner
Original Assignee
Sloning Biotechnology Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40996643&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE538200(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Sloning Biotechnology Gmbh filed Critical Sloning Biotechnology Gmbh
Application granted granted Critical
Publication of ATE538200T2 publication Critical patent/ATE538200T2/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT09004736T 2008-03-31 2009-03-31 Verfahren zur herstellung einer nukleinsäurenbibliothek ATE538200T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08006472 2008-03-31

Publications (1)

Publication Number Publication Date
ATE538200T2 true ATE538200T2 (de) 2012-01-15

Family

ID=40996643

Family Applications (1)

Application Number Title Priority Date Filing Date
AT09004736T ATE538200T2 (de) 2008-03-31 2009-03-31 Verfahren zur herstellung einer nukleinsäurenbibliothek

Country Status (8)

Country Link
US (1) US9115352B2 (de)
EP (1) EP2110435B2 (de)
JP (1) JP5642939B2 (de)
AT (1) ATE538200T2 (de)
DK (1) DK2110435T4 (de)
ES (1) ES2378790T5 (de)
PT (1) PT2110435E (de)
SI (2) SI2110435T2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012101347B4 (de) * 2012-02-20 2014-01-16 Markus Fuhrmann Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts
US10017770B2 (en) * 2014-07-03 2018-07-10 Ut-Battelle, Llc TNT cloning system
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
EP3802864A1 (de) 2018-06-06 2021-04-14 The Regents Of The University Of California Verfahren zur herstellung von nukleinsäurebibliotheken und zusammensetzungen und kits zur durchführung davon
WO2024222896A1 (zh) * 2023-04-27 2024-10-31 华大青兰生物科技(无锡)有限公司 靶向dna酶切方法

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
IL82064A0 (en) 1986-04-04 1987-10-20 Innogenetics Sa Nv Vectors comprising appropriate means for an enzymatical synthesis of nucleotide sequences,particularly dna fragments
US5093251A (en) * 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
DK0533838T3 (da) 1990-06-11 1998-02-23 Nexstar Pharmaceuticals Inc Nukleinsyreligander
WO1992013001A1 (en) 1991-01-18 1992-08-06 New York University A RECEPTOR TYROSINE KINASE TARGET PROTEIN cDNA CLONING METHOD AND hGRB PROTEINS
JPH07507201A (ja) 1992-03-10 1995-08-10 ザ　ガバメント　オブ　ザ　ユナイテッド　ステイツ　オブ　アメリカ，アズ　レプリゼンテッド　バイ　ザ　セクレタリー，デパートメント　オブ　ヘルス　アンド　ヒューマン　サービシーズ 可変鋳型反応
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
GB9618544D0 (en) 1996-09-05 1996-10-16 Brax Genomics Ltd Characterising DNA
US5770380A (en) * 1996-09-13 1998-06-23 University Of Pittsburgh Synthetic antibody mimics--multiple peptide loops attached to a molecular scaffold
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US5981190A (en) * 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
DE19736591A1 (de) * 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US20020048772A1 (en) * 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US8137906B2 (en) * 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (de) 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
WO2001061036A2 (en) 2000-02-17 2001-08-23 Complete Genomics As A method of mapping restriction endonuclease cleavage sites
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
JP2004508019A (ja) * 2000-07-28 2004-03-18 コンピュジェン　インコーポレイテッド トランスクリプトームの中に場所を占めるｒｎａ転写物及びスプライス変異体を検出するためのオリゴヌクレオチドライブラリー
ATE414767T1 (de) * 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nukleinsäure-linker und deren verwendung in der gensynthese
WO2003060084A2 (en) * 2002-01-14 2003-07-24 Diversa Corporation Methods for making polynucleotides and purifying double-stranded polynucleotides
US20030215837A1 (en) * 2002-01-14 2003-11-20 Diversa Corporation Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
ATE400650T1 (de) * 2002-10-18 2008-07-15 Sloning Biotechnology Gmbh Verfahren zur herstellung von nukleinsäuremolekülen

Also Published As

Publication number Publication date
US20100022410A1 (en) 2010-01-28
EP2110435B2 (de) 2016-05-18
ES2378790T3 (es) 2012-04-17
SI2110435T1 (sl) 2012-05-31
JP5642939B2 (ja) 2014-12-17
SI2110435T2 (sl) 2016-07-29
US9115352B2 (en) 2015-08-25
EP2110435A1 (de) 2009-10-21
PT2110435E (pt) 2012-03-15
ES2378790T5 (es) 2016-10-20
JP2009240310A (ja) 2009-10-22
EP2110435B1 (de) 2011-12-21
DK2110435T3 (da) 2012-04-10
DK2110435T4 (da) 2016-08-15

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