ATE400650T1 - Verfahren zur herstellung von nukleinsäuremolekülen - Google Patents

Verfahren zur herstellung von nukleinsäuremolekülen

Info

Publication number
ATE400650T1
ATE400650T1 AT02023385T AT02023385T ATE400650T1 AT E400650 T1 ATE400650 T1 AT E400650T1 AT 02023385 T AT02023385 T AT 02023385T AT 02023385 T AT02023385 T AT 02023385T AT E400650 T1 ATE400650 T1 AT E400650T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
recognition site
stranded
restriction enzyme
ligation product
Prior art date
Application number
AT02023385T
Other languages
English (en)
Inventor
Octavian Schatz
Timothy O'connell
Heinz Schwer
Thomas Waldmann
Original Assignee
Sloning Biotechnology Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloning Biotechnology Gmbh filed Critical Sloning Biotechnology Gmbh
Application granted granted Critical
Publication of ATE400650T1 publication Critical patent/ATE400650T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT02023385T 2002-10-18 2002-10-18 Verfahren zur herstellung von nukleinsäuremolekülen ATE400650T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02023385A EP1411122B1 (de) 2002-10-18 2002-10-18 Verfahren zur Herstellung von Nukleinsäuremolekülen

Publications (1)

Publication Number Publication Date
ATE400650T1 true ATE400650T1 (de) 2008-07-15

Family

ID=32039153

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02023385T ATE400650T1 (de) 2002-10-18 2002-10-18 Verfahren zur herstellung von nukleinsäuremolekülen

Country Status (9)

Country Link
US (1) US7695906B2 (de)
EP (1) EP1411122B1 (de)
AT (1) ATE400650T1 (de)
AU (1) AU2003278103A1 (de)
CA (1) CA2502431C (de)
DE (1) DE60227525D1 (de)
DK (1) DK1411122T3 (de)
ES (1) ES2310197T3 (de)
WO (1) WO2004035781A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
ATE414767T1 (de) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nukleinsäure-linker und deren verwendung in der gensynthese
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
ATE483020T1 (de) * 2004-01-23 2010-10-15 Sloning Biotechnology Gmbh Enzymatische herstellung von nukleinsäuremolekülen
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
WO2009082488A2 (en) 2007-12-24 2009-07-02 Bergenbio As Methods for creating and identifying functional rna interference elements
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
EP2373793B1 (de) 2008-12-18 2017-08-23 ITI Scotland Limited Verfahren zum zusammensetzen von polynukleinsäuresequenzen
JP2013504602A (ja) 2009-09-14 2013-02-07 ダイアックス コーポレーション 新しく設計したｈｃｃｄｒ３を含む遺伝子パッケージライブラリー
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
JP2013535987A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高レベルのエイコサペンタエン酸生産のための組換え微生物宿主細胞
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
LT3360963T (lt) 2010-11-12 2020-02-25 Gen9, Inc. Nukleorūgščių sintezės būdai ir įrenginiai
WO2013017950A1 (en) * 2011-07-29 2013-02-07 Cellectis High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules
US20130059296A1 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and Methods For High Fidelity Assembly of Nucleic Acids
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
DE102012101347B4 (de) * 2012-02-20 2014-01-16 Markus Fuhrmann Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
CN108913736B (zh) * 2018-07-10 2021-08-24 中国海洋大学 单链寡核苷酸的制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US6333149B1 (en) 1999-06-04 2001-12-25 Triad Biotechnology, Inc. NMR-solve method for rapid identification of bi-ligand drug candidates
DE19925862A1 (de) * 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
JP2001127864A (ja) 1999-10-29 2001-05-11 Suriibii Enterprises Japan:Kk 携帯電話機用イヤホンマイク及び携帯電話機

Also Published As

Publication number Publication date
CA2502431C (en) 2013-04-02
EP1411122A1 (de) 2004-04-21
EP1411122B1 (de) 2008-07-09
AU2003278103A1 (en) 2004-05-04
ES2310197T3 (es) 2009-01-01
DK1411122T3 (da) 2008-11-03
US20060194202A1 (en) 2006-08-31
US7695906B2 (en) 2010-04-13
DE60227525D1 (de) 2008-08-21
WO2004035781A1 (en) 2004-04-29
CA2502431A1 (en) 2004-04-29

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