WO2002083943A3 - Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen - Google Patents

Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen Download PDF

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Publication number
WO2002083943A3
WO2002083943A3 PCT/EP2002/004056 EP0204056W WO02083943A3 WO 2002083943 A3 WO2002083943 A3 WO 2002083943A3 EP 0204056 W EP0204056 W EP 0204056W WO 02083943 A3 WO02083943 A3 WO 02083943A3
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WO
WIPO (PCT)
Prior art keywords
dna fragments
dna
complex mixtures
microarray method
enriching
Prior art date
Application number
PCT/EP2002/004056
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English (en)
French (fr)
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WO2002083943A2 (de
Inventor
Juergen Distler
Original Assignee
Epigenomics Ag
Juergen Distler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Juergen Distler filed Critical Epigenomics Ag
Priority to US10/474,779 priority Critical patent/US20050009020A1/en
Priority to EP02737945A priority patent/EP1379700A2/de
Publication of WO2002083943A2 publication Critical patent/WO2002083943A2/de
Publication of WO2002083943A3 publication Critical patent/WO2002083943A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Beschrieben wird ein Verfahren zur gleichzeitigen, parallelen, selektiven Anreicherung von verschiedenen DNA-Abschnitten, die aus verschiedenen Geweben durch komplexe Amplifikationen erhalten werden und gewünschte individuelle Sequenzeigenschaften haben. Diese Eigenschaften der DNA-Fragmente, vor allem das Vorhandensein von 5-Methylcytosinen, sind durch Hybridisierung auf DNA Microarrays identifizierbar. Die Anreicherung der gewünschten DNA-Fragmente erfolgt durch mehrmalige Wiederholung der Arbeitsschritte (Hybridisierung, Dehybridisierung und Reamplifikation). Das Verfahren kombiniert das SELEX-Verfahren mit komplexen DNA-Arrays, die für die Anreicherung der DNA-Fragmente ververwendet werden. Die Amplifikate werden abschließend hinsichtlich ihrer Sequenz analysiert.
PCT/EP2002/004056 2001-04-12 2002-04-11 Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen WO2002083943A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/474,779 US20050009020A1 (en) 2001-04-12 2002-04-11 Microarray method for enriching dna fragments from complex mixtures
EP02737945A EP1379700A2 (de) 2001-04-12 2002-04-11 Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10119468.4 2001-04-12
DE10119468A DE10119468A1 (de) 2001-04-12 2001-04-12 Mikroarray-Verfahren zur Anreicherung von DNA-Fragmenten aus komplexen Mischungen

Publications (2)

Publication Number Publication Date
WO2002083943A2 WO2002083943A2 (de) 2002-10-24
WO2002083943A3 true WO2002083943A3 (de) 2003-10-30

Family

ID=7682144

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/004056 WO2002083943A2 (de) 2001-04-12 2002-04-11 Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen

Country Status (4)

Country Link
US (1) US20050009020A1 (de)
EP (1) EP1379700A2 (de)
DE (1) DE10119468A1 (de)
WO (1) WO2002083943A2 (de)

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DE10149947A1 (de) 2001-10-10 2003-04-17 Febit Ferrarius Biotech Gmbh Mikrofluidisches Extraktionsverfahren
US7300755B1 (en) * 2003-05-12 2007-11-27 Fred Hutchinson Cancer Research Center Methods for haplotyping genomic DNA
US20070048768A1 (en) * 2005-06-30 2007-03-01 Syngenta Participations Ag Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping and marker development
US20070192909A1 (en) * 2005-06-30 2007-08-16 Syngenta Participations Ag Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping ane marker development
DE102005034628B4 (de) * 2005-07-19 2007-08-23 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
US20070037169A1 (en) * 2005-08-09 2007-02-15 Combimatrix Corporation Selective Dehybridization using Electrochemically-Generated Reagent on an Electrode Microarray
US20070141604A1 (en) * 2005-11-15 2007-06-21 Gormley Niall A Method of target enrichment
EP2010657A2 (de) * 2006-04-24 2009-01-07 Nimblegen Systems, Inc. Verwendung von mikroarrays zur genomischen repräsentationsauswahl
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US20080194414A1 (en) * 2006-04-24 2008-08-14 Albert Thomas J Enrichment and sequence analysis of genomic regions
US8568979B2 (en) 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
US20080199861A1 (en) * 2007-02-15 2008-08-21 Honeywell International, Inc. Real-time microarray apparatus and methods related thereto
US8080372B2 (en) * 2007-04-11 2011-12-20 Canon Kabushiki Kaisha Method for detecting nucleic acid in sample, method for designing probes, system for designing probes therefor
US20090099040A1 (en) * 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
CN102203273A (zh) * 2008-09-09 2011-09-28 生命技术公司 生成基因特异性的文库的方法
US20100331204A1 (en) * 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
US10388049B2 (en) * 2017-04-06 2019-08-20 Honeywell International Inc. Avionic display systems and methods for generating avionic displays including aerial firefighting symbology

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Also Published As

Publication number Publication date
WO2002083943A2 (de) 2002-10-24
EP1379700A2 (de) 2004-01-14
DE10119468A1 (de) 2002-10-24
US20050009020A1 (en) 2005-01-13

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