ATE360065T1 - Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon - Google Patents
Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davonInfo
- Publication number
- ATE360065T1 ATE360065T1 AT02736176T AT02736176T ATE360065T1 AT E360065 T1 ATE360065 T1 AT E360065T1 AT 02736176 T AT02736176 T AT 02736176T AT 02736176 T AT02736176 T AT 02736176T AT E360065 T1 ATE360065 T1 AT E360065T1
- Authority
- AT
- Austria
- Prior art keywords
- protein synthesis
- dsdna
- fragment
- cell
- pcr
- Prior art date
Links
- 230000014616 translation Effects 0.000 title abstract 6
- 238000001243 protein synthesis Methods 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 10
- 102000053602 DNA Human genes 0.000 abstract 7
- 239000012634 fragment Substances 0.000 abstract 6
- 230000000692 anti-sense effect Effects 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001201356A JP4768155B2 (ja) | 2001-07-02 | 2001-07-02 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360065T1 true ATE360065T1 (de) | 2007-05-15 |
Family
ID=19038333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02736176T ATE360065T1 (de) | 2001-07-02 | 2002-06-24 | Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7195895B2 (de) |
| EP (1) | EP1411131B1 (de) |
| JP (1) | JP4768155B2 (de) |
| AT (1) | ATE360065T1 (de) |
| CA (1) | CA2452396A1 (de) |
| DE (1) | DE60219632T2 (de) |
| WO (1) | WO2003004703A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4768155B2 (ja) * | 2001-07-02 | 2011-09-07 | 独立行政法人理化学研究所 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
| JP4441170B2 (ja) * | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
| TW200526786A (en) * | 2003-02-10 | 2005-08-16 | Tatsuya Sawazaki | Automatic process for protein synthesis and apparatus therefor |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| JP4868731B2 (ja) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
| JP4590249B2 (ja) * | 2004-11-17 | 2010-12-01 | 独立行政法人理化学研究所 | 糖タンパク質合成用の無細胞タンパク質合成システム |
| JP4787488B2 (ja) * | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液 |
| US20070117179A1 (en) * | 2005-09-27 | 2007-05-24 | Invitrogen Corporation | In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles |
| EP2118129A4 (de) * | 2007-03-01 | 2010-04-28 | Life Technologies Corp | Isolierte phospholipidproteinpartikel |
| CA2700233A1 (en) * | 2007-10-22 | 2009-04-30 | Monoquant Pty Ltd. | A method of dna amplification |
| DK2213747T3 (da) * | 2007-11-05 | 2021-08-09 | Riken | Fremgangsmåde til fremstilling af membranprotein |
| KR20130023091A (ko) * | 2011-08-26 | 2013-03-07 | (주)바이오니아 | 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법 |
| JP6374409B2 (ja) * | 2014-01-27 | 2018-08-15 | 株式会社日立製作所 | 核酸増幅反応後の反応液の解析方法、解析装置及び核酸増幅反応後の反応液処理装置 |
| WO2017022696A1 (ja) | 2015-07-31 | 2017-02-09 | 国立研究開発法人理化学研究所 | 膜タンパク質の製造方法およびその利用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0491790A (ja) * | 1990-08-02 | 1992-03-25 | Shionogi & Co Ltd | ２段階ｐｃｒ法 |
| AU1322692A (en) * | 1990-11-05 | 1992-05-26 | United States Of America, As Represented By The Secretary Of The Army, The | Method for the in vitro production of protein from a dna sequence without cloning |
| WO1992011390A1 (en) * | 1990-12-17 | 1992-07-09 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
| JPH09234074A (ja) * | 1996-03-04 | 1997-09-09 | Sumitomo Electric Ind Ltd | アダプター二本鎖ｄｎａ及びそれを用いたｄｎａ増幅方法 |
| AU3152797A (en) * | 1996-06-07 | 1998-01-05 | Genentech Inc. | Methods for (in vitro) protein synthesis |
| US6136568A (en) * | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6607878B2 (en) * | 1997-10-06 | 2003-08-19 | Stratagene | Collections of uniquely tagged molecules |
| AU753732B2 (en) | 1997-10-23 | 2002-10-24 | Genzyme Corporation | Methods for detecting contamination in molecular diagnostics using PCR |
| AU4187799A (en) * | 1998-05-14 | 1999-11-29 | Clontech Laboratories, Inc. | Compositions and methods for protein purification based on metal ion affinity site |
| AU4032300A (en) * | 1999-03-24 | 2000-10-09 | Gene Therapy Systems, Inc. | Method for generating transcriptionally active dna fragments |
| US6303337B1 (en) * | 1999-08-25 | 2001-10-16 | Amber Gen. Inc. | N-terminal and C-terminal markers in nascent proteins |
| CA2421012A1 (en) * | 2000-08-30 | 2003-02-26 | Yaeta Endo | Designing and construction of transcription template for cell-free protein synthesis, and dilution batch wheat embryo cell-free protein synthesis method using the same |
| JP4768155B2 (ja) * | 2001-07-02 | 2011-09-07 | 独立行政法人理化学研究所 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
| DE10158904A1 (de) * | 2001-11-30 | 2003-06-12 | Roche Diagnostics Gmbh | Verfahren zur Herstellung von linearen DNA Fragmenten für die in vitro Expression von Proteinen |
| EP1394227B1 (de) | 2002-08-30 | 2006-05-24 | Eastman Kodak Company | Tintenzusammensetzung fuer den Tintenstrahldruck und Druckverfahren |
-
2001
- 2001-07-02 JP JP2001201356A patent/JP4768155B2/ja not_active Expired - Fee Related
-
2002
- 2002-06-24 AT AT02736176T patent/ATE360065T1/de not_active IP Right Cessation
- 2002-06-24 EP EP02736176A patent/EP1411131B1/de not_active Expired - Lifetime
- 2002-06-24 WO PCT/JP2002/006261 patent/WO2003004703A1/ja active IP Right Grant
- 2002-06-24 CA CA002452396A patent/CA2452396A1/en not_active Abandoned
- 2002-06-24 DE DE60219632T patent/DE60219632T2/de not_active Expired - Lifetime
-
2003
- 2003-12-31 US US10/748,055 patent/US7195895B2/en not_active Expired - Lifetime
-
2006
- 2006-03-14 US US11/374,114 patent/US7846694B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DE60219632T2 (de) | 2008-01-17 |
| US20040214292A1 (en) | 2004-10-28 |
| JP4768155B2 (ja) | 2011-09-07 |
| US20060147990A1 (en) | 2006-07-06 |
| US7846694B2 (en) | 2010-12-07 |
| JP2003009880A (ja) | 2003-01-14 |
| EP1411131A4 (de) | 2005-11-30 |
| WO2003004703A1 (en) | 2003-01-16 |
| EP1411131A1 (de) | 2004-04-21 |
| EP1411131B1 (de) | 2007-04-18 |
| US7195895B2 (en) | 2007-03-27 |
| DE60219632D1 (de) | 2007-05-31 |
| CA2452396A1 (en) | 2003-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE360065T1 (de) | Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon | |
| ATE400650T1 (de) | Verfahren zur herstellung von nukleinsäuremolekülen | |
| DE60323340D1 (de) | Verfahren zur herstellung interferierender rna-moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle | |
| ATE469223T1 (de) | Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden | |
| ATE547531T1 (de) | Verfahren zur herstellung von l-methionin unter verwendung coryneformer bakterien | |
| DE60228846D1 (de) | Neue mesogene, verfahren zu deren herstellung und verwendung | |
| DK1100880T3 (da) | Urat oxidase | |
| ATE418609T1 (de) | Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro | |
| ATE355382T1 (de) | Verfahren zur fermentativen herstellung von l- threonine | |
| DE3585953D1 (de) | Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine. | |
| DE602004029326D1 (de) | Enzymatische Herstellung von Nukleinsäuremolekülen | |
| DE60228847D1 (de) | ||
| DK1573058T3 (da) | Fremgangsmåde til forbedring af proteinproduktion | |
| ATE446299T1 (de) | Verfahren zur herstellung von 7-ethyl-10- hydroxycamptothecin | |
| WO2003097810A3 (en) | Nitrilases, nucleic acids encoding them and methods for making and using them | |
| ATE356871T1 (de) | Eine methode zur in vitro molekularen evolution der proteinfunktion | |
| ATE387497T1 (de) | Samenspezifische 7s-alpha-promoter zur expression von genen in pflanzen | |
| ATE442459T1 (de) | MIKROORGANISMUS MIT EINEM ßKNOCK-OUTß FADR GEN UND DESSEN VERWENDUNG IN EINEM VERFAHREN ZUR HERSTELLUNG VON L-THREONIN | |
| DE60129809D1 (de) | In vitro evolution von nukleinsäuren und kodierten polypeptiden | |
| ATE550437T1 (de) | Verfahren zur expression und reinigung von immunotoxinen | |
| DE60227335D1 (de) | Kontinuierliches Verfahren zur Modifizierung von Stärke und Stärkederivate mittels Verzweigungsenzyme | |
| ATE345315T1 (de) | Verfahren zur herstellung von expandiertem graphit | |
| DE69930610D1 (de) | Kaliumkanalprotein | |
| DE60234430D1 (de) | Halbdurchlässige verbundmembran, verfahren zur herstellung derselben und verfahren zur wasserbehandlung unter verwendung derselben | |
| ATE404547T1 (de) | Verfahren zur herstellung von propylenoxid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |