WO2003004703A1 - Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same - Google Patents

Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same Download PDF

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Publication number
WO2003004703A1
WO2003004703A1 PCT/JP2002/006261 JP0206261W WO03004703A1 WO 2003004703 A1 WO2003004703 A1 WO 2003004703A1 JP 0206261 W JP0206261 W JP 0206261W WO 03004703 A1 WO03004703 A1 WO 03004703A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna fragment
producing
protein
double
terminal domain
Prior art date
Application number
PCT/JP2002/006261
Other languages
English (en)
French (fr)
Inventor
Yoko Motoda
Takashi Yabuki
Takanori Kigawa
Shigeyuki Yokoyama
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Priority to CA002452396A priority Critical patent/CA2452396A1/en
Priority to EP02736176A priority patent/EP1411131B1/en
Priority to DE60219632T priority patent/DE60219632T2/de
Publication of WO2003004703A1 publication Critical patent/WO2003004703A1/ja
Priority to US10/748,055 priority patent/US7195895B2/en
Priority to US11/374,114 priority patent/US7846694B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/JP2002/006261 2001-07-02 2002-06-24 Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same WO2003004703A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002452396A CA2452396A1 (en) 2001-07-02 2002-06-24 Method of producing template dna and method of producing protein in cell-free protein synthesis system using the same
EP02736176A EP1411131B1 (en) 2001-07-02 2002-06-24 Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same
DE60219632T DE60219632T2 (de) 2001-07-02 2002-06-24 Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon
US10/748,055 US7195895B2 (en) 2001-07-02 2003-12-31 Method of producing template DNA and method of producing protein in cell-free protein synthesis system using the same
US11/374,114 US7846694B2 (en) 2001-07-02 2006-03-14 Process for producing template DNA and process for producing protein in cell-free protein synthesis system with the use of the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001201356A JP4768155B2 (ja) 2001-07-02 2001-07-02 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
JP2001-201356 2001-07-02

Publications (1)

Publication Number Publication Date
WO2003004703A1 true WO2003004703A1 (en) 2003-01-16

Family

ID=19038333

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/006261 WO2003004703A1 (en) 2001-07-02 2002-06-24 Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same

Country Status (7)

Country Link
US (2) US7195895B2 (ja)
EP (1) EP1411131B1 (ja)
JP (1) JP4768155B2 (ja)
AT (1) ATE360065T1 (ja)
CA (1) CA2452396A1 (ja)
DE (1) DE60219632T2 (ja)
WO (1) WO2003004703A1 (ja)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4768155B2 (ja) * 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
JP4441170B2 (ja) * 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
WO2004070047A1 (ja) * 2003-02-10 2004-08-19 Cellfree Sciences Co., Ltd. 自動蛋白質合成方法及びそれを行うための装置
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4590249B2 (ja) * 2004-11-17 2010-12-01 独立行政法人理化学研究所 糖タンパク質合成用の無細胞タンパク質合成システム
JP4787488B2 (ja) * 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
EP2118129A4 (en) * 2007-03-01 2010-04-28 Life Technologies Corp ISOLATED PHOSPHOLIPID PROTEIN PARTICLES
CN101849022A (zh) * 2007-10-22 2010-09-29 莫诺匡特私人有限公司 Dna扩增方法
JP5704677B2 (ja) * 2007-11-05 2015-04-22 独立行政法人理化学研究所 膜タンパク質の製造方法
KR20130023091A (ko) * 2011-08-26 2013-03-07 (주)바이오니아 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
US20160333397A1 (en) * 2014-01-27 2016-11-17 Hitachi, Ltd. Method and device for analyzing reaction liquid after nucleic acid amplification reaction, and device for processing reaction liquid after nucleic acid amplification reaction
CA3032514C (en) 2015-07-31 2021-05-04 Takehiro SHINODA Method of producing membrane protein and utilization thereof

Citations (5)

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Publication number Priority date Publication date Assignee Title
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
JPH09234074A (ja) * 1996-03-04 1997-09-09 Sumitomo Electric Ind Ltd アダプター二本鎖ｄｎａ及びそれを用いたｄｎａ増幅方法
WO1999014370A1 (en) * 1997-09-15 1999-03-25 Hiatt Andrew C De novo polynucleotide synthesis using rolling templates
WO1999020798A1 (en) * 1997-10-23 1999-04-29 Exact Laboratories, Inc. Methods for detecting contamination in molecular diagnostics using pcr

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WO1992007949A1 (en) * 1990-11-05 1992-05-14 The United States Of America, As Represented By The Secretary Of The Army Method for the in vitro production of protein from a dna sequence without cloning
AU3152797A (en) * 1996-06-07 1998-01-05 Genentech Inc. Methods for (in vitro) protein synthesis
US6607878B2 (en) * 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
WO1999057992A1 (en) * 1998-05-14 1999-11-18 Clontech Laboratories, Inc. Compositions and methods for protein purification based on a metal ion affinity site
JP2002539800A (ja) * 1999-03-24 2002-11-26 ジーン セラピー システムズ インコーポレーテッド 転写活性のあるｄｎａ断片を製造する方法
US6303337B1 (en) * 1999-08-25 2001-10-16 Amber Gen. Inc. N-terminal and C-terminal markers in nascent proteins
EP1314781A4 (en) 2000-08-30 2004-11-03 Endo Yaeta Dr DESIGN AND CONSTRUCTION OF A TRANSCRIPTION MODEL OF ACELLULAR PROTEIN SYNTHESIS, AND DISCONTINUOUS DILUTION METHOD FOR USE IN SYNTHESIS OF ACELLULAR WHEAT GERM PROTEIN
JP4768155B2 (ja) * 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
DE10158904A1 (de) * 2001-11-30 2003-06-12 Roche Diagnostics Gmbh Verfahren zur Herstellung von linearen DNA Fragmenten für die in vitro Expression von Proteinen
EP1394227B1 (en) 2002-08-30 2006-05-24 Eastman Kodak Company Ink jet ink composition and printing process

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
JPH09234074A (ja) * 1996-03-04 1997-09-09 Sumitomo Electric Ind Ltd アダプター二本鎖ｄｎａ及びそれを用いたｄｎａ増幅方法
WO1999014370A1 (en) * 1997-09-15 1999-03-25 Hiatt Andrew C De novo polynucleotide synthesis using rolling templates
WO1999020798A1 (en) * 1997-10-23 1999-04-29 Exact Laboratories, Inc. Methods for detecting contamination in molecular diagnostics using pcr

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KIGAWA T. ET AL.: "High-troughput cell-free protein expression system for structural genomics and proteomics studies", TANPAKUSHITSU KAKUSAN KOSO, vol. 47, no. 8, June 2002 (2002-06-01), pages 1014 - 1019, XP002956635 *

Also Published As

Publication number Publication date
EP1411131A4 (en) 2005-11-30
EP1411131B1 (en) 2007-04-18
JP4768155B2 (ja) 2011-09-07
US7195895B2 (en) 2007-03-27
CA2452396A1 (en) 2003-01-16
US7846694B2 (en) 2010-12-07
US20040214292A1 (en) 2004-10-28
DE60219632T2 (de) 2008-01-17
US20060147990A1 (en) 2006-07-06
ATE360065T1 (de) 2007-05-15
EP1411131A1 (en) 2004-04-21
DE60219632D1 (de) 2007-05-31
JP2003009880A (ja) 2003-01-14

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