AU1322692A - Method for the in vitro production of protein from a dna sequence without cloning - Google Patents

Method for the in vitro production of protein from a dna sequence without cloning

Info

Publication number
AU1322692A
AU1322692A AU13226/92A AU1322692A AU1322692A AU 1322692 A AU1322692 A AU 1322692A AU 13226/92 A AU13226/92 A AU 13226/92A AU 1322692 A AU1322692 A AU 1322692A AU 1322692 A AU1322692 A AU 1322692A
Authority
AU
Australia
Prior art keywords
cloning
protein
dna sequence
vitro production
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU13226/92A
Inventor
Kevin C Kain
David Ervin Lanar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Army
Original Assignee
US SEC TH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US SEC TH filed Critical US SEC TH
Publication of AU1322692A publication Critical patent/AU1322692A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU13226/92A 1990-11-05 1991-11-05 Method for the in vitro production of protein from a dna sequence without cloning Abandoned AU1322692A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60931890A 1990-11-05 1990-11-05
US609318 1996-02-29

Publications (1)

Publication Number Publication Date
AU1322692A true AU1322692A (en) 1992-05-26

Family

ID=24440279

Family Applications (1)

Application Number Title Priority Date Filing Date
AU13226/92A Abandoned AU1322692A (en) 1990-11-05 1991-11-05 Method for the in vitro production of protein from a dna sequence without cloning

Country Status (2)

Country Link
AU (1) AU1322692A (en)
WO (1) WO1992007949A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5709998A (en) * 1993-12-15 1998-01-20 The Johns Hopkins University Molecular diagnosis of familial adenomatous polyposis
FR2786788B1 (en) * 1998-12-08 2002-04-19 Proteus METHOD FOR SCREENING SUBSTANCES CAPABLE OF ALTERING THE ACTIVITY OF ONE OR MORE TARGET PROTEINS OR OF A TARGET SET OF PROTEINS EXPRESSED IN VITRO
US6911307B1 (en) 1998-12-08 2005-06-28 Proteus S.A. Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro
FR2786789B1 (en) * 1998-12-08 2001-12-28 Proteus METHOD FOR IN VITRO DETECTION OF A TARGET NUCLEIC ACID SEQUENCE IN A NUCLEIC ACID SAMPLE
FR2786787B1 (en) * 1998-12-08 2002-04-19 Proteus METHOD OF IN VITRO ANALYSIS OF A KNOWN PHENOTYPE FROM A SAMPLE OF NUCLEIC ACIDS
US6818396B1 (en) 2000-11-28 2004-11-16 Proteus S.A. Process for determination of the activity of a substance using an in vitro functional test
DE10106339A1 (en) * 2001-02-09 2002-08-22 Hans-Joachim Mueller Process for high throughput screening of candidate compounds via high throughput direct synthesis of recombinant polypeptides
DE10113265B4 (en) * 2001-03-16 2018-03-08 Qiagen Gmbh Use of a stabilized nucleic acid for the production of a protein
JP4768155B2 (en) * 2001-07-02 2011-09-07 独立行政法人理化学研究所 Method for producing template DNA and method for producing protein by cell-free protein synthesis system using the same
JP4441170B2 (en) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Escherichia coli cell extract having mutation in S12 ribosomal protein and cell-free protein production method using the same
JP4868731B2 (en) 2004-11-17 2012-02-01 独立行政法人理化学研究所 Cell-free protein synthesis system derived from cultured mammalian cells
JP4787488B2 (en) 2004-11-19 2011-10-05 独立行政法人理化学研究所 Cell-free protein synthesis method using linear template DNA and cell extract therefor
EP2213747B1 (en) 2007-11-05 2021-06-30 Riken Method for producing membrane protein
CN107002078A (en) * 2014-10-09 2017-08-01 生命技术公司 CRISPR oligonucleotides and gene editing
WO2017022696A1 (en) 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 Method of manufacturing membrane protein and utilization thereof
AU2020329166A1 (en) 2019-08-09 2022-03-03 Nutcracker Therapeutics, Inc. Microfluidic apparatus and methods of use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction

Also Published As

Publication number Publication date
WO1992007949A1 (en) 1992-05-14

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