DE60129809D1 - In vitro evolution von nukleinsäuren und kodierten polypeptiden - Google Patents

In vitro evolution von nukleinsäuren und kodierten polypeptiden

Info

Publication number
DE60129809D1
DE60129809D1 DE60129809T DE60129809T DE60129809D1 DE 60129809 D1 DE60129809 D1 DE 60129809D1 DE 60129809 T DE60129809 T DE 60129809T DE 60129809 T DE60129809 T DE 60129809T DE 60129809 D1 DE60129809 D1 DE 60129809D1
Authority
DE
Germany
Prior art keywords
nucleic acid
protein
cognate pair
nucleic acids
vitro evolution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60129809T
Other languages
English (en)
Other versions
DE60129809T2 (de
Inventor
Richard B Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE60129809D1 publication Critical patent/DE60129809D1/de
Publication of DE60129809T2 publication Critical patent/DE60129809T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60129809T 2000-05-19 2001-05-18 In vitro evolution von nukleinsäuren und kodierten polypeptiden Expired - Lifetime DE60129809T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20601600P 2000-05-19 2000-05-19
US206016P 2000-05-19
PCT/US2001/016210 WO2001090414A1 (en) 2000-05-19 2001-05-18 In vitro evolution of nucleic acids and encoded polypeptide

Publications (2)

Publication Number Publication Date
DE60129809D1 true DE60129809D1 (de) 2007-09-20
DE60129809T2 DE60129809T2 (de) 2008-04-30

Family

ID=22764634

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60129809T Expired - Lifetime DE60129809T2 (de) 2000-05-19 2001-05-18 In vitro evolution von nukleinsäuren und kodierten polypeptiden

Country Status (8)

Country Link
EP (1) EP1290222B1 (de)
JP (1) JP2003533995A (de)
AT (1) ATE369442T1 (de)
AU (2) AU2001261771B2 (de)
CA (1) CA2409305C (de)
DE (1) DE60129809T2 (de)
HK (1) HK1054410B (de)
WO (1) WO2001090414A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
CN101006177B (zh) 2002-03-15 2011-10-12 纽韦卢森公司 改善的用于合成模制分子的方法
US10730906B2 (en) 2002-08-01 2020-08-04 Nuevolutions A/S Multi-step synthesis of templated molecules
ES2640279T3 (es) 2002-10-30 2017-11-02 Nuevolution A/S Procedimiento para la síntesis de un complejo bifuncional
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
WO2004074429A2 (en) 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
DE602004023960D1 (de) 2003-09-18 2009-12-17 Nuevolution As Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen
AU2004314705A1 (en) * 2003-12-12 2005-08-11 Proteonova, Inc. System and methods for nucleic acid and polypeptide selection
US10206998B2 (en) 2005-01-12 2019-02-19 Proteonova, Inc. Modular targeted therapeutic agents and methods of making same
WO2006076417A1 (en) * 2005-01-12 2006-07-20 Proteonova, Inc. Method for making targeted therapeutic agents
SI2341140T1 (sl) 2005-12-01 2017-12-29 Nuevolution A/S Postopki encimskega kodiranja za učinkovito sintezo velikih knjižnic
CA2677804C (en) * 2007-02-12 2017-03-14 Proteonova, Inc. Generation of library of soluble random polypeptides linked to mrna
LT2558577T (lt) 2010-04-16 2019-03-12 Nuevolution A/S Bifunkciniai kompleksai ir tokių kompleksų gamybos ir naudojimo būdai
GB2500243A (en) * 2012-03-15 2013-09-18 Isogenica Ltd Identifying members of immobilised peptide libraries comprising protein-DNA complexes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599303A (en) * 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
US5843701A (en) * 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
WO1992002536A1 (en) * 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation
US5462733A (en) * 1993-02-04 1995-10-31 Yale University Immune system modulation using psoralens activated with visible light
RU2233878C2 (ru) * 1997-01-21 2004-08-10 Дзе Дженерал Хоспитал Корпорейшн Способ отбора желательного белка и нуклеиновой кислоты, средства для его осуществления
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays

Also Published As

Publication number Publication date
CA2409305C (en) 2011-01-11
AU2001261771B2 (en) 2005-06-09
HK1054410B (zh) 2008-03-07
EP1290222A1 (de) 2003-03-12
DE60129809T2 (de) 2008-04-30
CA2409305A1 (en) 2001-11-29
EP1290222A4 (de) 2005-12-14
AU6177101A (en) 2001-12-03
HK1054410A1 (en) 2003-11-28
JP2003533995A (ja) 2003-11-18
WO2001090414A1 (en) 2001-11-29
ATE369442T1 (de) 2007-08-15
EP1290222B1 (de) 2007-08-08

Similar Documents

Publication Publication Date Title
DE60129809D1 (de) In vitro evolution von nukleinsäuren und kodierten polypeptiden
DE60142254D1 (de) Zusammensetzungen, methoden und reagentien zur nukleinsäureisolierung mittels oberflächenaktiven substanzen und proteasen
DE69835143D1 (de) Selektion von proteinen mittels rns-protein fusionen
DE60230672D1 (de) Nukleinsäureanreicherung
DE69834032D1 (de) Verfahren zur erkennung von nucleinsäuremolekülen codierend für (poly)peptide, welche mit zielmolekülen interagieren
WO1999064592A3 (en) Green fluorescent proteins for measuring the ph of a biological sample
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
DE60119170D1 (de) Nachweismethode mittels verstärkter silberfärbung
DE60144145D1 (de) Subtilisin-variante
ATE418609T1 (de) Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro
DE60117761D1 (de) Luciferase, fluoreszenzproteine, für luciferase und fluoreszenzproteine kodierend nukleinsaüre und ihre verwendung in der diagnose, hoch-durchsatz screeningsverfahren
WO2001096373A3 (en) Fluorescent timer proteins and methods for their use
DE60139544D1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
DE60114804D1 (de) Konstruierte fluoreszenzproteine mit langen wellenlängen
ATE360065T1 (de) Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon
DE60140139D1 (de) Auf nukleinsäurenamplifizierung basierendes verfahren zur bestimmung von methylierungsprofil und reagenzien dafür
DE60026733D1 (de) Histone-deacetylase-8 proteine, nukleinsäuren und methoden zur verwendung
DE69728897D1 (de) Saeugetierchemokine
DE602004011228D1 (de) Verfahren und zusammensetzungen zur bestimmung glycierter proteine
PT1322753E (pt) Novas redutases do acido 2,5-diceto-l-gluconico e metodos de utilizacao
DE50008101D1 (de) Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
ATE510012T1 (de) Herstellung von kalibratoren und ihre verwendung zur quantifizierung von besonderen nukleotidsequenzen
ATE414905T1 (de) Diabetesmodell
DE69937453D1 (de) Verfahren zur isolierung und charakterisierung potentieller funktionen ausgehend von einer biologischen probe, welche nukleinsäuren enthält

Legal Events

Date Code Title Description
8364 No opposition during term of opposition