WO2004042034A3 - Method for the linear amplification of complementary dna - Google Patents

Method for the linear amplification of complementary dna Download PDF

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Publication number
WO2004042034A3
WO2004042034A3 PCT/US2003/035350 US0335350W WO2004042034A3 WO 2004042034 A3 WO2004042034 A3 WO 2004042034A3 US 0335350 W US0335350 W US 0335350W WO 2004042034 A3 WO2004042034 A3 WO 2004042034A3
Authority
WO
WIPO (PCT)
Prior art keywords
template
cdna
synthesizing
molecule
molecules
Prior art date
Application number
PCT/US2003/035350
Other languages
French (fr)
Other versions
WO2004042034A2 (en
Inventor
Era L Pogosova-Agadjanyan
Derek L Stirewalt
Original Assignee
Hutchinson Fred Cancer Res
Era L Pogosova-Agadjanyan
Derek L Stirewalt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hutchinson Fred Cancer Res, Era L Pogosova-Agadjanyan, Derek L Stirewalt filed Critical Hutchinson Fred Cancer Res
Priority to AU2003291325A priority Critical patent/AU2003291325A1/en
Publication of WO2004042034A2 publication Critical patent/WO2004042034A2/en
Publication of WO2004042034A3 publication Critical patent/WO2004042034A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

In one aspect, the present invention provides methods for linearly amplifying cDNA from an RNA molecule. The methods comprise the steps of: (a) synthesizing an antisense cDNA molecule using an RNA molecule as a template; (b) synthesizing an extended antisense cDNA molecule using a template-switching oligonucleotide as a template; (c) synthesizing multiple copies of sense cDNA molecules using the extended antisense cDNA strand as a template; and (d) generating double-stranded cDNA molecules using the multiple copies of sense cDNA molecules as templates. Another aspect of the invention provides methods for linearly amplifying RNA, comprising the steps o£ (a) using an RNA molecule as a template for synthesizing an antisense cDNA molecule; (b) using a template-switching oligonucleotide as a template for synthesizing an extended antisense cDNA molecule; (c) using the extended antisense cDNA molecule as a template for synthesizing multiple copies of sense cDNA molecules; (d) using the sense cDNA molecules as templates for generating double-stranded cDNA molecules; and (e) using the double-stranded cDNA molecules as templates for synthesizing RNA molecules.
PCT/US2003/035350 2002-11-04 2003-11-04 Method for the linear amplification of complementary dna WO2004042034A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003291325A AU2003291325A1 (en) 2002-11-04 2003-11-04 Method for the linear amplification of complementary dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42373102P 2002-11-04 2002-11-04
US60/423,731 2002-11-04

Publications (2)

Publication Number Publication Date
WO2004042034A2 WO2004042034A2 (en) 2004-05-21
WO2004042034A3 true WO2004042034A3 (en) 2005-02-10

Family

ID=32312706

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/035350 WO2004042034A2 (en) 2002-11-04 2003-11-04 Method for the linear amplification of complementary dna

Country Status (2)

Country Link
AU (1) AU2003291325A1 (en)
WO (1) WO2004042034A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2621146A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
EP2454271A4 (en) 2009-07-15 2015-08-12 Univ California Peptides whose uptake in cells is controllable

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PHILLIPS J: "Antisense RNA Amplification: A linear Amplification Method for Analyzing the mRNA Population from Single Living Cells", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 10, 1996, pages 283 - 288, XP002248904 *

Also Published As

Publication number Publication date
AU2003291325A8 (en) 2004-06-07
AU2003291325A1 (en) 2004-06-07
WO2004042034A2 (en) 2004-05-21

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