WO2005021786A8 - A method of sequencing nucleic acids by ligation of labelled oligonucleotides - Google Patents

A method of sequencing nucleic acids by ligation of labelled oligonucleotides

Info

Publication number
WO2005021786A8
WO2005021786A8 PCT/GB2004/003666 GB2004003666W WO2005021786A8 WO 2005021786 A8 WO2005021786 A8 WO 2005021786A8 GB 2004003666 W GB2004003666 W GB 2004003666W WO 2005021786 A8 WO2005021786 A8 WO 2005021786A8
Authority
WO
WIPO (PCT)
Prior art keywords
method
ligation
nucleic acids
sequencing nucleic
labelled oligonucleotides
Prior art date
Application number
PCT/GB2004/003666
Other languages
French (fr)
Other versions
WO2005021786A1 (en
Inventor
Colin Barnes
Original Assignee
Colin Barnes
Solexa Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to GB0320059.9 priority Critical
Priority to GB0320059A priority patent/GB0320059D0/en
Application filed by Colin Barnes, Solexa Ltd filed Critical Colin Barnes
Publication of WO2005021786A1 publication Critical patent/WO2005021786A1/en
Publication of WO2005021786A8 publication Critical patent/WO2005021786A8/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The invention is concerned with a method of sequencing nucleic acid molecules, such as DNA molecules, using ligation cassettes.
PCT/GB2004/003666 2003-08-27 2004-08-27 A method of sequencing nucleic acids by ligation of labelled oligonucleotides WO2005021786A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0320059.9 2003-08-27
GB0320059A GB0320059D0 (en) 2003-08-27 2003-08-27 A method of sequencing

Publications (2)

Publication Number Publication Date
WO2005021786A1 WO2005021786A1 (en) 2005-03-10
WO2005021786A8 true WO2005021786A8 (en) 2005-05-26

Family

ID=28686409

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/003666 WO2005021786A1 (en) 2003-08-27 2004-08-27 A method of sequencing nucleic acids by ligation of labelled oligonucleotides

Country Status (2)

Country Link
GB (1) GB0320059D0 (en)
WO (1) WO2005021786A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9217177B2 (en) 2005-02-01 2015-12-22 Applied Biosystems, Llc Methods for bead-based sequencing

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2233582A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
PL2963127T3 (en) 2006-04-04 2018-01-31 Keygene Nv High throughput detection of molecular markers based on restriction fragments
US8178300B2 (en) 2006-07-12 2012-05-15 Keygene N.V. Method for the identification of the clonal source of a restriction fragment
CA2718905A1 (en) 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
ES2403312T3 (en) 2009-01-13 2013-05-17 Keygene N.V. New strategies for genome sequencing
WO2012034007A2 (en) 2010-09-10 2012-03-15 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
DK2729580T3 (en) 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
CN105229173A (en) 2013-03-08 2016-01-06 豪夫迈·罗氏有限公司 EGFR suddenlys change blood testing
EP2818867A1 (en) 2013-06-27 2014-12-31 INSERM (Institut National de la Santé et de la Recherche Medicale) Antibodies conjugated to at least one nucleic acid molecule and their use in multiplex immuno-detection assays
EP3016973A1 (en) 2013-07-05 2016-05-11 INSERM - Institut National de la Santé et de la Recherche Médicale Novel alternative splice transcripts for mhc class i related chain alpha (mica) and uses thereof
JP2018526018A (en) 2015-09-11 2018-09-13 インセルム(インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル) Non-invasive method for assessing gene integrity of pluripotent stem cells
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US6401267B1 (en) * 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
JP2001508308A (en) * 1997-01-15 2001-06-26 ブラックス グループ リミテッド Nucleic acid sequencing

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9217177B2 (en) 2005-02-01 2015-12-22 Applied Biosystems, Llc Methods for bead-based sequencing
US9493830B2 (en) 2005-02-01 2016-11-15 Applied Biosystems, Llc Reagents, methods, and libraries for bead-based sequencing

Also Published As

Publication number Publication date
GB0320059D0 (en) 2003-10-01
WO2005021786A1 (en) 2005-03-10

Similar Documents

Publication Publication Date Title
WO2001092565A8 (en) Diagnosis of diseases associated dna transcription by means of assessing the methylation status of genes associated with dna transcription
AU2003299541A8 (en) Microfluidic nucleic acid analysis
WO2006116362A3 (en) Structured substrates for optical surface profiling
AU2002325538A1 (en) Nucleic acid amplification methods
PT1488039E (en) Marcacao apparatus, for the marking of nucleic acid items
WO2005118806A3 (en) METHODS AND COMPOSITIONS INVOLVING MicroRNA
EP1448581A4 (en) Methods for detection and quantification of analytes in complex mixtures
WO2007014045A3 (en) Methods for rapid identification and quantitation of nucleic acid variants
EP1138783A3 (en) Method for the efficiency-corrected real-time quantification of nucleic acids
ZA200406664B (en) Method for the seperation of acids from chemical reaction mixtures by means of ionic fluids
AU2003272203A1 (en) Multiplex nucleic acid reactions
AU8182301A (en) Method for the production of acrolein or acrylic acid or the mixture thereof from propane
WO2006076022A3 (en) Nano-pcr: methods and devices for nucleic acid amplification and detection
BR0116033A (en) Isothermal amplification of nucleic acids on a solid support
WO2006031745A3 (en) Methods for long-range sequence analysis of nucleic acids
AT506448T (en) Method for the production of l-amino acid by fermentation
WO2002052031A3 (en) Nucleic acid amplification
EP1629084A4 (en) Modulation of c-reactive protein expression
AU6152301A (en) Detection of nucleic acid hybridization by fluorescence polarization
WO2002038806A3 (en) Detection of nucleic acid polymorphisms
DE60222307D1 (en) Method for enrichment of nucleic acid molecules
WO2012162267A3 (en) Nucleic acid encoding reactions
WO2005059096A3 (en) Methods for high fidelity production of long nucleic acid molecules with error control
AU2002365912A1 (en) Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
WO2003027326A3 (en) Nucleic acid sequencing by raman monitoring of molecular deconstruction

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
CFP Corrected version of a pamphlet front page

Free format text: UNDER (54) PUBLISHED TITLE REPLACED BY CORRECT TITLE

122 Ep: pct application non-entry in european phase