ATE315666T1 - Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren - Google Patents

Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Info

Publication number
ATE315666T1
ATE315666T1 AT01978771T AT01978771T ATE315666T1 AT E315666 T1 ATE315666 T1 AT E315666T1 AT 01978771 T AT01978771 T AT 01978771T AT 01978771 T AT01978771 T AT 01978771T AT E315666 T1 ATE315666 T1 AT E315666T1
Authority
AT
Austria
Prior art keywords
dna
nucleic acid
acid primer
modified
template
Prior art date
Application number
AT01978771T
Other languages
English (en)
Inventor
Thomas Valentine Mccarthy
Ruairi Collins
Original Assignee
Univ College Cork Nat Univ Ie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ College Cork Nat Univ Ie filed Critical Univ College Cork Nat Univ Ie
Application granted granted Critical
Publication of ATE315666T1 publication Critical patent/ATE315666T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
AT01978771T 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren ATE315666T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IE20000887A IE20000887A1 (en) 2000-11-03 2000-11-03 Method for the amplification and optional characterisation of nucleic acids

Publications (1)

Publication Number Publication Date
ATE315666T1 true ATE315666T1 (de) 2006-02-15

Family

ID=11042688

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01978771T ATE315666T1 (de) 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Country Status (13)

Country Link
US (1) US20040067559A1 (de)
EP (1) EP1330546B1 (de)
JP (1) JP2004512843A (de)
CN (1) CN1473201A (de)
AT (1) ATE315666T1 (de)
AU (2) AU2002210862B2 (de)
CA (1) CA2427474A1 (de)
DE (1) DE60116651T2 (de)
DK (1) DK1330546T3 (de)
ES (1) ES2254505T3 (de)
IE (1) IE20000887A1 (de)
RU (1) RU2284357C2 (de)
WO (1) WO2002036821A2 (de)

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US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
KR101293713B1 (ko) 2004-12-03 2013-08-12 휴먼 제네틱 시그너처스 피티와이 엘티디 시토신의 화학적 변형에 의한 미생물 핵산의 단순화 방법
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CN101203618B (zh) 2005-05-26 2013-03-13 人类遗传标记控股有限公司 使用含有非常规碱基的引物的等温链置换扩增
CA2616241C (en) * 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CN101292046B (zh) 2005-09-14 2013-03-13 人类遗传标记控股有限公司 健康状态的测定法
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
US20070218478A1 (en) * 2005-12-16 2007-09-20 Affymetrix, Inc. Methods for fragmentation and analysis of nucleic acid
JP5654749B2 (ja) * 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
AU2007298650B2 (en) 2006-05-04 2013-10-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US20130323795A1 (en) * 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
US9279150B2 (en) 2007-01-10 2016-03-08 General Electric Company Mutant endonuclease V enzymes and applications thereof
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
US20100092972A1 (en) * 2007-03-16 2010-04-15 Human Genetic Signatures Pty Ltd. Assay for gene expression
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
CN101918595A (zh) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 用于扩增和复制亚硫酸氢盐修饰的核酸的酶
CN101903521A (zh) * 2007-12-20 2010-12-01 人类遗传标记控股有限公司 核酸扩增相关污染物的清除
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
CN102296116A (zh) * 2011-09-02 2011-12-28 北京大学 对dna目标序列进行信号放大和检测的方法
US9732375B2 (en) 2011-09-07 2017-08-15 Human Genetic Signatures Pty. Ltd. Molecular detection assay using direct treatment with a bisulphite reagent
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
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US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
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AU2014205110A1 (en) * 2013-01-13 2015-08-27 Unitaq Bio Methods and compositions for PCR using blocked and universal primers
US9068218B2 (en) * 2013-01-18 2015-06-30 Emerald Therapeutics, Inc. Rotationally sequestered translators
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
EP3277833B1 (de) 2015-03-30 2019-01-09 H. Hoffnabb-La Roche Ag Verfahren zum amplifizieren von höchst gleichmässigen und weniger fehleranfälligen nukleinsäurebibliotheken
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN112424351A (zh) * 2018-07-30 2021-02-26 奥利希罗基因组学有限公司 在无细胞体系中对dna进行编辑的方法
CN117980494A (zh) * 2021-09-17 2024-05-03 曼彻斯特大学 寡核苷酸的合成
CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法
CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用

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US6399309B1 (en) * 2000-12-07 2002-06-04 Becton, Dickinson And Company Amplification and detection of mycoplasma pneumoniae targeting the ORF9 region of the hmw gene cluster

Also Published As

Publication number Publication date
ES2254505T3 (es) 2006-06-16
AU2002210862B2 (en) 2006-06-08
DE60116651T2 (de) 2006-09-14
IE20000887A1 (en) 2002-12-11
DK1330546T3 (da) 2006-05-15
DE60116651D1 (de) 2006-04-06
CA2427474A1 (en) 2002-05-10
JP2004512843A (ja) 2004-04-30
CN1473201A (zh) 2004-02-04
EP1330546B1 (de) 2006-01-11
US20040067559A1 (en) 2004-04-08
AU1086202A (en) 2002-05-15
WO2002036821A3 (en) 2003-03-27
RU2284357C2 (ru) 2006-09-27
WO2002036821A2 (en) 2002-05-10
EP1330546A2 (de) 2003-07-30

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