ATE315666T1 - Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren - Google Patents

Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Info

Publication number
ATE315666T1
ATE315666T1 AT01978771T AT01978771T ATE315666T1 AT E315666 T1 ATE315666 T1 AT E315666T1 AT 01978771 T AT01978771 T AT 01978771T AT 01978771 T AT01978771 T AT 01978771T AT E315666 T1 ATE315666 T1 AT E315666T1
Authority
AT
Austria
Prior art keywords
dna
nucleic acid
acid primer
modified
template
Prior art date
Application number
AT01978771T
Other languages
English (en)
Inventor
Thomas Valentine Mccarthy
Ruairi Collins
Original Assignee
Univ College Cork Nat Univ Ie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ College Cork Nat Univ Ie filed Critical Univ College Cork Nat Univ Ie
Application granted granted Critical
Publication of ATE315666T1 publication Critical patent/ATE315666T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
AT01978771T 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren ATE315666T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IE20000887A IE20000887A1 (en) 2000-11-03 2000-11-03 Method for the amplification and optional characterisation of nucleic acids

Publications (1)

Publication Number Publication Date
ATE315666T1 true ATE315666T1 (de) 2006-02-15

Family

ID=11042688

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01978771T ATE315666T1 (de) 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Country Status (13)

Country Link
US (1) US20040067559A1 (de)
EP (1) EP1330546B1 (de)
JP (1) JP2004512843A (de)
CN (1) CN1473201A (de)
AT (1) ATE315666T1 (de)
AU (2) AU2002210862B2 (de)
CA (1) CA2427474A1 (de)
DE (1) DE60116651T2 (de)
DK (1) DK1330546T3 (de)
ES (1) ES2254505T3 (de)
IE (1) IE20000887A1 (de)
RU (1) RU2284357C2 (de)
WO (1) WO2002036821A2 (de)

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WO2005014808A1 (ja) * 2003-06-30 2005-02-17 Matsushita Electric Industrial Co., Ltd. ヌクレオチド鎖修飾方法
CN1863927B (zh) 2003-09-04 2010-08-11 人类遗传标记控股有限公司 核酸检测分析
US20050136417A1 (en) * 2003-12-19 2005-06-23 Affymetrix, Inc. Amplification of nucleic acids
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
NZ555620A (en) 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
WO2006125267A1 (en) * 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US7435561B2 (en) * 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CA2621932A1 (en) * 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for hpv and methylated genomic dna targets to screen for cervical cancer
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
US20070218478A1 (en) * 2005-12-16 2007-09-20 Affymetrix, Inc. Methods for fragmentation and analysis of nucleic acid
US20100173364A1 (en) * 2006-04-11 2010-07-08 New England Biolabs, Inc. Repair of Nucleic Acids for Improved Amplification
EP2029782B1 (de) 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US9279150B2 (en) 2007-01-10 2016-03-08 General Electric Company Mutant endonuclease V enzymes and applications thereof
US20130323795A1 (en) * 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
CA2680426A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
US9388457B2 (en) * 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8685675B2 (en) * 2007-11-27 2014-04-01 Human Genetic Signatures Pty. Ltd. Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009079703A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
CN102296116A (zh) * 2011-09-02 2011-12-28 北京大学 对dna目标序列进行信号放大和检测的方法
WO2013033749A1 (en) 2011-09-07 2013-03-14 Human Genetic Signatures Pty Ltd Molecular detection assay
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2943590A4 (de) * 2013-01-13 2017-02-15 Unitaq Bio Verfahren und zusammensetzungen für pcr mittels blockierter und universelle primer
US9068218B2 (en) * 2013-01-18 2015-06-30 Emerald Therapeutics, Inc. Rotationally sequestered translators
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
WO2016156071A1 (en) 2015-03-30 2016-10-06 F. Hoffmann-La Roche Ag Methods to amplify highly uniform and less error prone nucleic acid libraries
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
RU2766717C1 (ru) * 2018-07-30 2022-03-15 Орикиро Дженомикс, Инк. Способ редактирования днк в бесклеточной системе
CN117980494A (zh) * 2021-09-17 2024-05-03 曼彻斯特大学 寡核苷酸的合成
CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法
CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用

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US6399309B1 (en) * 2000-12-07 2002-06-04 Becton, Dickinson And Company Amplification and detection of mycoplasma pneumoniae targeting the ORF9 region of the hmw gene cluster

Also Published As

Publication number Publication date
AU1086202A (en) 2002-05-15
CA2427474A1 (en) 2002-05-10
EP1330546B1 (de) 2006-01-11
IE20000887A1 (en) 2002-12-11
DK1330546T3 (da) 2006-05-15
RU2284357C2 (ru) 2006-09-27
AU2002210862B2 (en) 2006-06-08
WO2002036821A2 (en) 2002-05-10
CN1473201A (zh) 2004-02-04
US20040067559A1 (en) 2004-04-08
DE60116651T2 (de) 2006-09-14
ES2254505T3 (es) 2006-06-16
JP2004512843A (ja) 2004-04-30
DE60116651D1 (de) 2006-04-06
EP1330546A2 (de) 2003-07-30
WO2002036821A3 (en) 2003-03-27

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