DE60116651D1 - Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren - Google Patents

Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Info

Publication number
DE60116651D1
DE60116651D1 DE60116651T DE60116651T DE60116651D1 DE 60116651 D1 DE60116651 D1 DE 60116651D1 DE 60116651 T DE60116651 T DE 60116651T DE 60116651 T DE60116651 T DE 60116651T DE 60116651 D1 DE60116651 D1 DE 60116651D1
Authority
DE
Germany
Prior art keywords
dna
nucleic acid
acid primer
modified
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60116651T
Other languages
English (en)
Other versions
DE60116651T2 (de
Inventor
Valentine Mccarthy
Ruairi Collins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Cork
Original Assignee
University College Cork
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University College Cork filed Critical University College Cork
Publication of DE60116651D1 publication Critical patent/DE60116651D1/de
Application granted granted Critical
Publication of DE60116651T2 publication Critical patent/DE60116651T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60116651T 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren Expired - Fee Related DE60116651T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IE20000887 2000-11-03
IE20000887A IE20000887A1 (en) 2000-11-03 2000-11-03 Method for the amplification and optional characterisation of nucleic acids
PCT/IE2001/000139 WO2002036821A2 (en) 2000-11-03 2001-11-01 Method for the amplification and optional characterisation of nucleic acids

Publications (2)

Publication Number Publication Date
DE60116651D1 true DE60116651D1 (de) 2006-04-06
DE60116651T2 DE60116651T2 (de) 2006-09-14

Family

ID=11042688

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60116651T Expired - Fee Related DE60116651T2 (de) 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Country Status (13)

Country Link
US (1) US20040067559A1 (de)
EP (1) EP1330546B1 (de)
JP (1) JP2004512843A (de)
CN (1) CN1473201A (de)
AT (1) ATE315666T1 (de)
AU (2) AU1086202A (de)
CA (1) CA2427474A1 (de)
DE (1) DE60116651T2 (de)
DK (1) DK1330546T3 (de)
ES (1) ES2254505T3 (de)
IE (1) IE20000887A1 (de)
RU (1) RU2284357C2 (de)
WO (1) WO2002036821A2 (de)

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US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
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IE20020544A1 (en) * 2002-06-28 2003-12-31 Univ College Cork Nat Univ Ie Method for the characterisation of nucleic acid molecules
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DE602004019926D1 (de) * 2003-06-30 2009-04-23 Panasonic Corp Verfahren zur modifizierung einer nukleotidkette
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US20050136417A1 (en) * 2003-12-19 2005-06-23 Affymetrix, Inc. Amplification of nucleic acids
JP2007524407A (ja) 2003-12-29 2007-08-30 ニューゲン テクノロジーズ， インコーポレイテッド 核酸のメチル化状態を分析するための方法、ならびに核酸の断片化、標識化および固定化のための方法
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
EP1828411B1 (de) 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Verfahren zur vereinfachung mikrobieller nukleinsäuren durch chemische modifikation von cytosinen
EP1866434B1 (de) 2005-02-19 2013-06-05 Avacta Group plc Isotherme nukleinsäureamplifikation
CN101203618B (zh) * 2005-05-26 2013-03-13 人类遗传标记控股有限公司 使用含有非常规碱基的引物的等温链置换扩增
EP1929049B1 (de) 2005-07-25 2013-04-10 Alere San Diego, Inc. Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung
US8343738B2 (en) 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
US20070218478A1 (en) * 2005-12-16 2007-09-20 Affymetrix, Inc. Methods for fragmentation and analysis of nucleic acid
JP5654749B2 (ja) * 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
EP2029782B1 (de) 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US20130323795A1 (en) * 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
US9279150B2 (en) 2007-01-10 2016-03-08 General Electric Company Mutant endonuclease V enzymes and applications thereof
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
AU2008229628A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009067743A1 (en) * 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
EP2432795B1 (de) 2009-05-20 2015-03-04 Alere San Diego, Inc. Dna-glycosylase/lyase- und ap-endonukleasesubstrate
CN102296116A (zh) * 2011-09-02 2011-12-28 北京大学 对dna目标序列进行信号放大和检测的方法
MY167564A (en) 2011-09-07 2018-09-14 Human Genetic Signatures Pty Ltd Molecular detection assay
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2943590A4 (de) * 2013-01-13 2017-02-15 Unitaq Bio Verfahren und zusammensetzungen für pcr mittels blockierter und universelle primer
US9068218B2 (en) * 2013-01-18 2015-06-30 Emerald Therapeutics, Inc. Rotationally sequestered translators
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
WO2016156071A1 (en) * 2015-03-30 2016-10-06 F. Hoffmann-La Roche Ag Methods to amplify highly uniform and less error prone nucleic acid libraries
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
KR102520700B1 (ko) * 2018-07-30 2023-04-10 오리시로 제노믹스 가부시키가이샤 무세포계에서 dna를 편집하는 방법
WO2023041931A1 (en) * 2021-09-17 2023-03-23 The University Of Manchester Synthesis of oligonucleotides
CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法
CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用

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US5683896A (en) * 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU708821B2 (en) * 1995-07-11 1999-08-12 Forfas Glycosylase mediated detection of nucleotide sequences at candidate loci
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
AU753661B2 (en) * 1998-04-22 2002-10-24 Enterprise Ireland Trading As Bioresearch Ireland A method for the characterisation of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site
US6399309B1 (en) * 2000-12-07 2002-06-04 Becton, Dickinson And Company Amplification and detection of mycoplasma pneumoniae targeting the ORF9 region of the hmw gene cluster

Also Published As

Publication number Publication date
WO2002036821A2 (en) 2002-05-10
AU1086202A (en) 2002-05-15
EP1330546B1 (de) 2006-01-11
ATE315666T1 (de) 2006-02-15
DK1330546T3 (da) 2006-05-15
EP1330546A2 (de) 2003-07-30
US20040067559A1 (en) 2004-04-08
AU2002210862B2 (en) 2006-06-08
CA2427474A1 (en) 2002-05-10
JP2004512843A (ja) 2004-04-30
CN1473201A (zh) 2004-02-04
WO2002036821A3 (en) 2003-03-27
RU2284357C2 (ru) 2006-09-27
DE60116651T2 (de) 2006-09-14
ES2254505T3 (es) 2006-06-16
IE20000887A1 (en) 2002-12-11

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8339 Ceased/non-payment of the annual fee