MX353621B - Variantes de alfa-amilasa. - Google Patents

Variantes de alfa-amilasa.

Info

Publication number
MX353621B
MX353621B MX2014000065A MX2014000065A MX353621B MX 353621 B MX353621 B MX 353621B MX 2014000065 A MX2014000065 A MX 2014000065A MX 2014000065 A MX2014000065 A MX 2014000065A MX 353621 B MX353621 B MX 353621B
Authority
MX
Mexico
Prior art keywords
alpha
variants
amylase variants
relates
present
Prior art date
Application number
MX2014000065A
Other languages
English (en)
Other versions
MX2014000065A (es
Inventor
Peter Friis Esben
Kaasgaard Svend
Oebro Jens
Eskildsen Larsen Signe
Svendsen Allan
Helle Johansen Annette
Skjoet Michael
Andersen Carsten
Beier Lars
Duarte Guilherme Pereira Toscano Miguel
Bjoernvad Mads
Winther Rasmussen Frank
Spaangner Christiansen Liv
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2014000065A publication Critical patent/MX2014000065A/es
Publication of MX353621B publication Critical patent/MX353621B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Abstract

La presente invención se refiere a variantes de una alfa-amilasa original. La presente invención también se refiere a polinucleótidos que codifican las variantes; a constructos de ácidos nucleicos, vectores y células hospederas que comprenden los polinucleótidos; y a métodos para utilizar las variantes. Una variante aislada de una alfa-amilasa original, caracterizad porque comprende por lo menos dos sustituciones en posiciones que comprenden a 140 a 206 del polipéptido de la SEQ ID NO: 1, en donde la variante tiene por lo menos 90% de identidad secuencial con el polipéptido de la SEQ ID NO: 1 y tiene actividad de alfa-amilasa y en donde la sustitución en la posición 206 es V206Y.
MX2014000065A 2011-06-30 2012-06-29 Variantes de alfa-amilasa. MX353621B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11172251 2011-06-30
PCT/EP2012/062748 WO2013001078A1 (en) 2011-06-30 2012-06-29 Alpha-amylase variants

Publications (2)

Publication Number Publication Date
MX2014000065A MX2014000065A (es) 2014-02-17
MX353621B true MX353621B (es) 2018-01-22

Family

ID=46397293

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000065A MX353621B (es) 2011-06-30 2012-06-29 Variantes de alfa-amilasa.

Country Status (13)

Country Link
US (5) US9434932B2 (es)
EP (3) EP3798303A1 (es)
JP (1) JP6204352B2 (es)
KR (1) KR20140056237A (es)
CN (2) CN103649307B (es)
AU (1) AU2012277721B2 (es)
BR (1) BR112013033782B1 (es)
DK (2) DK2726607T3 (es)
ES (2) ES2834102T3 (es)
IN (1) IN2014CN00650A (es)
MX (1) MX353621B (es)
WO (1) WO2013001078A1 (es)
ZA (1) ZA201400678B (es)

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