WO2014195356A3 - Alpha-amylase variants and polynucleotides encoding same - Google Patents

Alpha-amylase variants and polynucleotides encoding same Download PDF

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Publication number
WO2014195356A3
WO2014195356A3 PCT/EP2014/061572 EP2014061572W WO2014195356A3 WO 2014195356 A3 WO2014195356 A3 WO 2014195356A3 EP 2014061572 W EP2014061572 W EP 2014061572W WO 2014195356 A3 WO2014195356 A3 WO 2014195356A3
Authority
WO
WIPO (PCT)
Prior art keywords
alpha
polynucleotides encoding
amylase variants
encoding same
variants
Prior art date
Application number
PCT/EP2014/061572
Other languages
French (fr)
Other versions
WO2014195356A2 (en
Inventor
Carsten Andersen
Original Assignee
Novozymes A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes A/S filed Critical Novozymes A/S
Priority to CN202210257926.3A priority Critical patent/CN114634921B/en
Priority to CN201480031631.8A priority patent/CN105264058B/en
Priority to EP14733537.6A priority patent/EP3004315A2/en
Priority to EP20192786.0A priority patent/EP3786269A1/en
Priority to US14/896,300 priority patent/US10538751B2/en
Publication of WO2014195356A2 publication Critical patent/WO2014195356A2/en
Publication of WO2014195356A3 publication Critical patent/WO2014195356A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
PCT/EP2014/061572 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same WO2014195356A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN202210257926.3A CN114634921B (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same
CN201480031631.8A CN105264058B (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same
EP14733537.6A EP3004315A2 (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same
EP20192786.0A EP3786269A1 (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same
US14/896,300 US10538751B2 (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13170894 2013-06-06
EP13170894.3 2013-06-06

Publications (2)

Publication Number Publication Date
WO2014195356A2 WO2014195356A2 (en) 2014-12-11
WO2014195356A3 true WO2014195356A3 (en) 2015-03-26

Family

ID=48569990

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2014/061572 WO2014195356A2 (en) 2013-06-06 2014-06-04 Alpha-amylase variants and polynucleotides encoding same

Country Status (4)

Country Link
US (1) US10538751B2 (en)
EP (2) EP3786269A1 (en)
CN (2) CN105264058B (en)
WO (1) WO2014195356A2 (en)

Families Citing this family (10)

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EP3978604A1 (en) 2013-03-11 2022-04-06 Danisco US Inc. Alpha-amylase combinatorial variants
DK179660B1 (en) 2016-04-08 2019-03-13 Novozymes A/S Stabilized Alpha-Amylase Variants and use of the same
WO2020074499A1 (en) 2018-10-09 2020-04-16 Novozymes A/S Cleaning compositions and uses thereof
WO2020074498A1 (en) 2018-10-09 2020-04-16 Novozymes A/S Cleaning compositions and uses thereof
WO2020193532A1 (en) * 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
CA3160401A1 (en) * 2019-12-19 2021-06-24 Neil Joseph Lant Cleaning compositions comprising polypeptides having alpha amylase activity
WO2021133701A1 (en) 2019-12-23 2021-07-01 The Procter & Gamble Company Compositions comprising enzymes
WO2021130167A1 (en) 2019-12-23 2021-07-01 Novozymes A/S Enzyme compositions and uses thereof
EP4093842A1 (en) 2020-01-23 2022-11-30 Novozymes A/S Enzyme compositions and uses thereof
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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