KR20190069603A - 펩티도미메틱 거대고리 - Google Patents

펩티도미메틱 거대고리 Download PDF

Info

Publication number
KR20190069603A
KR20190069603A KR1020197016502A KR20197016502A KR20190069603A KR 20190069603 A KR20190069603 A KR 20190069603A KR 1020197016502 A KR1020197016502 A KR 1020197016502A KR 20197016502 A KR20197016502 A KR 20197016502A KR 20190069603 A KR20190069603 A KR 20190069603A
Authority
KR
South Korea
Prior art keywords
mod
res
term
artificial sequence
carbon
Prior art date
Application number
KR1020197016502A
Other languages
English (en)
Other versions
KR102104762B1 (ko
Inventor
빈센트 겔라바
노리유키 가와하타
Original Assignee
에일러론 테라퓨틱스 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에일러론 테라퓨틱스 인코포레이티드 filed Critical 에일러론 테라퓨틱스 인코포레이티드
Publication of KR20190069603A publication Critical patent/KR20190069603A/ko
Application granted granted Critical
Publication of KR102104762B1 publication Critical patent/KR102104762B1/ko

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C07K7/52—Cyclic peptides containing at least one abnormal peptide link with only normal peptide links in the ring
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/12—Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C07K7/54—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring
    • C07K7/56—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring the cyclisation not occurring through 2,4-diamino-butanoic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

본 발명은 신규 펩티도미메틱 거대고리, 및 질환의 치료를 위한 이러한 거대고리의 사용 방법을 제공한다.

Description

펩티도미메틱 거대고리{PEPTIDOMIMETIC MACROCYCLES}
관련 출원에 대한 상호참조
본원은 2010년 8월 13일자로 출원된 미국 가출원 제61/373,701호, 2010년 8월 13일자로 출원된 미국 가출원 제61/373,638호 및 2010년 8월 16일자로 출원된 미국 가출원 제61/374,163호(이들은 전체적으로 본원에 참고로 도입됨)의 우선권 이익을 주장한다.
서열 목록
본원은 EFS-Web을 통해 ASCII 포맷으로 제출되었고 이로써 그 전체가 본원에 참고로 인용되는 서열 목록을 포함한다. 2013년 7월 31일에 생성된 상기 ASCII 카피는 파일명이 35224-757.601_SL.txt이며, 크기가 586,273 바이트이다.
인간 전사 인자 단백질 p53은 DNA 손상 및 세포 스트레스에 반응하여 세포 주기 정지 및 아폽토시스를 유도함으로써 악성 형질전환으로부터 세포를 보호하는 데 있어서 매우 중요한 역할을 수행한다. E3 유비퀴틴 리가제(ubiquitin ligase) HDM2는 p53 전사활성화(transactivation) 활성을 중화하는 직접적인 결합 상호작용을 통해 p53 기능을 음성적으로 조절하고, p53 단백질을 핵으로부터 유출시키고 유비퀴틸화-프로테아좀(ubiquitylation-proteasomal) 경로를 통한 분해를 위해 p53을 표적화한다. 결실, 돌연변이 또는 HDM2 과발현에 의한 p53 활성의 손실은 인간 암에서 가장 흔한 결함이다. 야생형 p53을 발현하는 종양은 활성 p53의 농도를 안정화시키거나 증가시키는 약제에 의해 공격받기 쉽다. 이와 관련하여, HDM2 활성의 억제는 p53 활성을 회복하고 암 세포를 시험관 및 생체 내에서 아폽토시스에 다시 민감하게 만드는 검증된 방법으로서 알려졌다. HDMX(HDM4)는 p53의 유사한 음성 조절제로서 보다 최근에 확인되었고, 연구는 HDM2의 p53 결합 경계면과 HDMX의 p53 결합 경계면 사이에 상당한 구조적 상동성을 보여주었다.
p53-HDM2 및 p53-HDMX 단백질-단백질 상호작용은 HDM2 및 HDMX의 표면 상의 소수성 틈새(clefts) 내로 삽입되는, p53의 동일한 15개 잔기 α 나선형 전사활성화 도메인에 의해 매개된다. p53의 이 도메인 내의 3개 잔기(F19, W23 및 L26)는 HDM2 및 HDMX와의 결합에 필수적이다. 본 발명은 p53과 HDM2의 상호작용, p53과 HDMX의 상호작용, 또는 p53과 HDM2 및 HDMX 단백질 둘 다와의 상호작용을 억제함으로써 p53의 활성을 조절하고 암 및 다른 과증식성 질환을 포함하나 이들로 한정되지 않는 질환의 치료를 위해 사용될 수 있는, p53에 기초한 펩티도미메틱 거대고리를 제공한다.
인간 p53의 일부와 관련된 안정하게 가교결합된 펩티드("p53 펩티도미메틱 거대고리")가 이하에 기재된다. 이들 가교결합된 펩티드는 p53과 HDM2의 결합 및 p53과 HDMX의 결합에 중요한 것으로 생각되는 p53의 일부의 α 나선형 2차 구조를 안정화시키는 데에 도움이 될 수 있는 분자내 가교결합을 함께 형성하는 2개 이상의 변형된 아미노산을 함유한다. 따라서, 본원에 기재된 가교결합된 폴리펩티드는 가교결합되지 않은 상응하는 폴리펩티드에 비해 상대적으로 개선된 생물학적 활성을 가질 수 있다. p53 펩티도미메틱 거대고리는 p53과 HDM2의 결합 및/또는 p53과 HDMX의 결합을 방해하여 기능성 p53을 유리시키고 그의 파괴를 억제하는 것으로 생각된다. 본원에 기재된 p53 펩티도미메틱 거대고리는 치료적으로, 예를 들면, p53의 바람직하지 않게 낮은 수준 또는 낮은 활성을 특징으로 하는 암 및 다른 장애의 치료, 및/또는 HDM2 또는 HDMX의 바람직하지 않게 높은 활성 수준을 특징으로 하는 암 및 다른 장애의 치료를 위해 사용될 수 있다. p53 펩티도미메틱 거대고리는 과도한 세포 생존 및 증식의 병태, 예컨대, 암 및 자가면역뿐만 아니라, 부적절한 세포 주기 정지 및 아폽토시스의 병태, 예컨대, 신경퇴행 및 면역결핍을 초래하는, p53 전사 경로의 파괴된 조절과 관련된 임의의 장애의 치료에도 유용할 수 있다. 몇몇 경우, p53 펩티도미메틱 거대고리는 HDM2(예를 들면, 진뱅크(GenBank)® 등록번호: 228952; GI:228952) 및/또는 HDMX(HDM4로도 지칭됨; 진뱅크® 등록번호: 88702791; GI:88702791)에 결합한다.
한 양태에서, 본 발명은 표 1, 2, 3 또는 4의 아미노산 서열들로 구성된 군으로부터 선택된 아미노산 서열과 약 60%, 80%, 90% 또는 95% 이상 동일한 아미노산 서열을 포함하는 펩티도미메틱 거대고리를 제공한다. 대안적으로, 상기 펩티도미메틱 거대고리의 아미노산 서열은 표 1의 아미노산 서열들로 구성된 군으로부터 선택된다. 대안적으로, 상기 펩티도미메틱 거대고리의 아미노산 서열은 전술된 바와 같이 선택되고, 추가로 상기 거대고리는 티오에테르 또는 트리아졸을 포함하지 않는다. 몇몇 실시양태에서, 상기 펩티도미메틱 거대고리는 나선, 예컨대, α 나선을 포함한다. 다른 실시양태에서, 상기 펩티도미메틱 거대고리는 α,α-이치환된 아미노산을 포함한다. 본 발명의 펩티도미메틱 거대고리는 2개 이상의 아미노산의 α 위치들을 연결하는 가교제(crosslinker)를 포함할 수 있다. 상기 2개의 아미노산들 중 하나 이상은 α,α-이치환된 아미노산일 수 있다.
몇몇 실시양태에서, 상기 펩티도미메틱 거대고리는 하기 화학식 I로 표시된다:
[화학식 I]
Figure pat00001
상기 식에서,
A, C, D 및 E는 각각 독립적으로 천연 또는 비천연 아미노산이고, 말단 D 및 E는 독립적으로 임의적으로 캡핑(capping) 기를 포함하고;
B는 천연 또는 비천연 아미노산, 아미노산 유사체(analog),
Figure pat00002
[-NH-L3-CO-], [-NH-L3-SO2-] 또는 [-NH-L3-]이고;
R1 및 R2는 독립적으로 비치환되거나 할로로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 사이클로알킬알킬, 헤테로알킬, 헤테로사이클로알킬 또는 -H이고;
R3은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 헤테로알킬, 사이클로알킬, 헤테로사이클로알킬, 사이클로알킬알킬, 사이클로아릴, 헤테로사이클로아릴 또는 수소이고;
L은 화학식 -L1-L2-의 거대고리 형성 연결제(linker)이고;
L1 및 L2는 독립적으로 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 사이클로아릴렌, 헤테로사이클로아릴렌 또는 [-R4-K-R4-]n이고, 이때 이들 각각은 임의적으로 R5로 치환되고;
R4는 각각 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 아릴렌 또는 헤테로아릴렌이고;
K는 각각 O, S, SO, SO2, CO, CO2 또는 CONR3이고;
R5는 각각 독립적으로 할로겐, 알킬, -OR6, -N(R6)2, -SR6, -SOR6, -SO2R6, -CO2R6, 형광 모이어티(moiety), 방사성 동위원소 또는 치료제이고;
R6은 각각 독립적으로 -H, 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R7은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이거나, D 잔기를 갖는 환형 구조의 일부이고;
R8은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이거나, E 잔기를 갖는 환형 구조의 일부이고;
v 및 w는 독립적으로 1 내지 1000의 정수이고;
u는 1 내지 10의 정수이고;
x, y 및 z는 독립적으로 0 내지 10의 정수이고;
n은 1 내지 5의 정수이다.
다양한 실시양태에서, 펩티도미메틱 거대고리는 L1 및 L2를 포함하고, 이때 L1 및 L2는 단독으로 또는 조합으로 티오에테르 또는 트리아졸을 포함하지 않는다.
다른 실시양태에서, 펩티도미메틱 거대고리는 펩티도미메틱 거대고리 내에서 제1 아미노산의 골격 아미노 기를 제2 아미노산에 연결하는 가교제를 포함할 수 있다. 예를 들면, 본 발명은 하기 화학식 IV 또는 IVa의 펩티도미메틱 거대고리를 제공한다:
[화학식 IV]
Figure pat00003
[화학식 IVa]
Figure pat00004
상기 식에서,
A, C, D 및 E는 각각 독립적으로 천연 또는 비천연 아미노산이고, 말단 D 및 E는 독립적으로 임의적으로 캡핑 기를 포함하고;
B는 천연 또는 비천연 아미노산, 아미노산 유사체,
Figure pat00005
[-NH-L3-CO-], [-NH-L3-SO2-] 또는 [-NH-L3-]이고;
R1 및 R2는 독립적으로 비치환되거나 할로로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 사이클로알킬알킬, 헤테로알킬, 헤테로사이클로알킬 또는 -H이거나, E 잔기를 갖는 환형 구조의 일부이고;
R3은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 헤테로알킬, 사이클로알킬, 헤테로사이클로알킬, 사이클로알킬알킬, 사이클로아릴, 헤테로사이클로아릴 또는 수소이고;
L1 및 L2는 독립적으로 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 사이클로아릴렌, 헤테로사이클로아릴렌 또는 [-R4-K-R4-]n이고, 이때 이들 각각은 임의적으로 R5로 치환되고;
R4는 각각 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 아릴렌 또는 헤테로아릴렌이고;
K는 각각 O, S, SO, SO2, CO, CO2 또는 CONR3이고;
R5는 각각 독립적으로 할로겐, 알킬, -OR6, -N(R6)2, -SR6, -SOR6, -SO2R6, -CO2R6, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R6은 각각 독립적으로 -H, 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R7은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이고;
v 및 w는 독립적으로 1 내지 1000의 정수이고;
u는 1 내지 10의 정수이고;
x, y 및 z는 독립적으로 0 내지 10의 정수이고;
n은 1 내지 5의 정수이다.
추가로, 본 발명은 본 발명의 펩티도미메틱 거대고리를 대상체에게 투여하는 단계를 포함하는, 상기 대상체에서 암을 치료하는 방법을 제공한다. 또한, 본 발명의 펩티도미메틱 거대고리를 대상체에게 투여하는 단계를 포함하는, 상기 대상체에서 p53, HDM2 또는 HDMX의 활성을 조절하는 방법, 또는 이러한 펩티도미메틱 거대고리를 대상체에게 투여하는 단계를 포함하는, 상기 대상체에서 p53과 HDM2 단백질 사이 및/또는 p53과 HDMX 단백질 사이의 상호작용을 길항하는 방법이 제공된다.
참조에 의한 도입
본 명세서에서 언급된 모든 공개문헌, 특허 및 특허출원은 각각의 개별 공개문헌, 특허 또는 특허출원이 참고로 도입되는 것으로 구체적으로 및 개별적으로 표시되어 있는 것과 동일한 정도로 본원에 참고로 도입된다.
본 발명의 신규 특징은 첨부된 특허청구범위에 구체적으로 기재되어 있다. 본 발명의 특징 및 장점은 본 발명의 원리가 이용되는 예시적 실시양태를 기술하는 하기 상세한 설명 및 첨부된 도면을 참조하면 더 잘 이해될 것이다.
도 1은 Fmoc-Me-6-클로로-트립토판 및 Fmoc-6-클로로-트립토판의 합성을 보여준다.
도 2는 Me-6-클로로-(Boc)트립토판-Ni-S-BPB의 LC-MS 트레이스를 보여준다.
도 3은 Me-6-클로로-(Boc)트립토판-Ni-S-BPB의 1H-NMR 스펙트럼을 보여준다.
도 4는 Fmoc-Me-6-클로로-(Boc)트립토판의 LC-MS 트레이스를 보여준다.
도 5는 Fmoc-Me-6-클로로-(Boc)트립토판의 1H-NMR 스펙트럼을 보여준다.
도 6a 내지 6f는 본 발명의 예시적 펩티도미메틱 거대고리(기재된 순서대로 각각 서열 번호 38-178)에 대한 세포 생존능 분석, 경쟁 ELISA 분석, GRIP 분석, Kd 데이터, p21 활성화 분석, 형광 편광 경쟁 결합 및 원형 나선도(helicity) 데이터의 결과를 보여준다.
도 7a 내지 7d는 다양한 거대고리들로부터의 데이터를 제공한다(도 7a 및 7b는 기재된 순서대로 각각 서열 번호 42, 163, 177, 214, 217, 344, 289-290, 383, 533, 529, 543, 601, 544, 594, 279, 374 및 660을 개시하고, 도 7c 및 7d는 기재된 순서대로 각각 서열 번호 702, 699, 704, 706, 689, 507, 624, 703, 716, 606, 605, 642, 691, 731, 375, 727, 662, 587 및 714를 개시한다).
도 8a 및 8b는 다양한 거대고리들로부터의 데이터를 제공한다.
본원에서 사용된 바와 같이, 용어 "거대고리(macrocycle)"는 9개 이상의 공유결합된 원자들에 의해 형성된 고리 또는 환을 포함하는 화학구조를 갖는 분자를 지칭한다.
본원에서 사용된 바와 같이, 용어 "펩티도미메틱 거대고리(peptidomimetic macrocycle)" 또는 "가교결합된 폴리펩티드(crosslinked polypeptide)"는 복수의 펩티드 결합에 의해 연결된 복수의 아미노산 잔기, 및 동일 분자 내에서 제1 천연 발생 또는 비천연 발생 아미노산 잔기(또는 유사체)와 제2 천연 발생 또는 비천연 발생 아미노산 잔기(또는 유사체) 사이에 거대고리를 형성하는 하나 이상의 거대고리 형성 연결제를 포함하는 화합물을 지칭한다. 펩티도미메틱 거대고리는 거대고리 형성 연결제가 제1 아미노산 잔기(또는 유사체)의 α 탄소를 제2 아미노산 잔기(또는 유사체)의 α 탄소에 연결하는 실시양태를 포함한다. 펩티도미메틱 거대고리는 임의적으로 하나 이상의 아미노산 잔기 및/또는 아미노산 유사체 잔기 사이의 하나 이상의 비펩티드 결합을 포함하고, 임의적으로 거대고리를 형성하는 임의의 비천연 발생 아미노산 잔기 이외에 하나 이상의 비천연 발생 아미노산 잔기 또는 아미노산 유사체 잔기를 포함한다. 펩티도미메틱 거대고리와 관련하여 언급될 때 "상응하는 비가교결합된 폴리펩티드"는 상기 거대고리와 동일한 길이를 갖고 상기 거대고리에 상응하는 야생형 서열의 등가 천연 아미노산을 포함하는 폴리펩티드에 관한 것으로 이해된다.
본원에서 사용된 바와 같이, 용어 "안정성"은 원이색법(circular dichroism), NMR 또는 또 다른 생물리학적 수단에 의해 측정되었을 때 본 발명의 펩티도미메틱 거대고리에 의한 용액에서의 정의된 2차 구조의 유지, 또는 시험관내 또는 생체내 단백질용해성 분해에 대한 내성을 지칭한다. 본 발명에서 고려되는 2차 구조의 비한정적 예는 α 나선, β 회전(turn) 및 β 주름 시트(pleated sheet)이다.
본원에서 사용된 바와 같이, 용어 "나선 안정성"은 원이색법 또는 NMR에 의해 측정되었을 때 본 발명의 펩티도미메틱 거대고리에 의한 α 나선 구조의 유지를 지칭한다. 예를 들면, 몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 원이색법에 의해 측정되었을 때 상응하는 비가교결합된 거대고리에 비해 α 나선도에서의 1.25배, 1.5배, 1.75배 또는 2배 이상의 증가를 나타낸다.
용어 "α 아미노산" 또는 단순히 "아미노산"은 α 탄소로 지정된 탄소에 결합된 아미노 기 및 카복실 기 둘 다를 함유하는 분자를 지칭한다. 적합한 아미노산은 천연 발생 아미노산뿐만 아니라 유기 합성 또는 다른 대사 경로에 의해 제조된 비천연 발생 아미노산의 D 이성질체 및 L 이성질체 둘 다를 포함하나 이들로 한정되지 않는다. 문맥이 구체적으로 달리 표시하지 않은 한, 본원에서 사용된 바와 같이 용어 아미노산은 아미노산 유사체를 포함하기 위한 것이다.
용어 "천연 발생 아미노산"은 자연에서 합성된 펩티드에서 통상적으로 발견되고 1 문자 약어 A, R, N, C, D, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y 및 V로 공지되어 있는 20개의 아미노산들 중 어느 하나를 지칭한다.
용어 "아미노산 유사체" 또는 "비천연 아미노산"은 아미노산과 구조적으로 유사하고 펩티도미메틱 거대고리의 형성에 있어서 아미노산을 치환시킬 수 있는 분자를 지칭한다. 아미노산 유사체는 하나 이상의 추가 메틸렌 기가 아미노 기와 카복실 기 사이에 포함되어 있다는 점(예를 들면, α 아미노 β 카복시 산), 또는 아미노 또는 카복시 기가 유사한 반응성 기로 치환되어 있다는 점(예를 들면, 2차 또는 3차 아민에 의한 1차 아민의 치환, 또는 에스테르에 의한 카복시 기의 치환)을 제외하고 본원에 정의된 바와 같은 아미노산과 구조적으로 동일한 화합물을 포함하나 이로 한정되지 않는다. 비천연 아미노산은 하기 화학식에 따른 구조를 포함한다:
Figure pat00006
Figure pat00007
Figure pat00008
"비필수" 아미노산 잔기는 폴리펩티드의 필수적인 생물학적 또는 생화학적 활성(예를 들면, 수용체 결합 또는 활성화)을 제거하거나 실질적으로 변경하지 않으면서 상기 폴리펩티드의 야생형 서열로부터 변경될 수 있는 잔기이다. "필수" 아미노산 잔기는 폴리펩티드의 야생형 서열로부터 변경되었을 때 상기 폴리펩티드의 필수적인 생물학적 또는 생화학적 활성을 제거하거나 실질적으로 제거하는 잔기이다.
"보존적 아미노산 치환"은 아미노산 잔기가 유사한 측쇄를 갖는 아미노산 잔기로 교체되는 치환이다. 유사한 측쇄를 갖는 아미노산 잔기의 패밀리는 당업계에서 정의되어 있다. 이들 패밀리는 염기성 측쇄를 갖는 아미노산(예를 들면, K, R, H), 산성 측쇄를 갖는 아미노산(예를 들면, D, E), 비하전된 극성 측쇄를 갖는 아미노산(예를 들면, G, N, Q, S, T, Y, C), 비극성 측쇄를 갖는 아미노산(예를 들면, A, V, L, I, P, F, M, W), 베타 분지된 측쇄를 갖는 아미노산(예를 들면, T, V, I) 및 방향족 측쇄를 갖는 아미노산(예를 들면, Y, F, W, H)을 포함한다. 따라서, 폴리펩티드 내의 예측된 비필수 아미노산 잔기는 예를 들면, 바람직하게는 동일한 측쇄 패밀리로부터의 또 다른 아미노산 잔기로 교체된다. 허용가능한 치환의 다른 예는 등전자 고려에 기초한 치환(예를 들면, 메티오닌 대신에 노르류신) 또는 다른 성질에 기초한 치환(예를 들면, 페닐알라닌 대신에 티오에닐알라닌)이다.
용어 "캡핑 기"는 본 펩티도미메틱 거대고리의 폴리펩티드 쇄의 카복시 또는 아미노 말단에 존재하는 화학적 모이어티를 지칭한다. 카복시 말단의 캡핑 기는 비변형된 카복실산(즉, -COOH) 또는 치환기를 갖는 카복실산을 포함한다. 예를 들면, 카복시 말단은 아미노 기로 치환되어 C 말단에서 카복스아미드를 생성할 수 있다. 다양한 치환기들은 PEG화된(pegylated) 2차 아민을 포함하는 1차 아민 및 2차 아민을 포함하나 이들로 한정되지 않는다. C 말단에 대한 대표적인 2차 아민 캡핑 기는 하기 기들을 포함한다:
Figure pat00009
Figure pat00010
아미노 말단의 캡핑 기는 비변형된 아민(즉, -NH2) 또는 치환기를 갖는 아민을 포함한다. 예를 들면, 아미노 말단은 아실 기로 치환되어 N 말단에서 카복스아미드를 생성할 수 있다. 다양한 치환기들은 C1-C6 카보닐, C7-C30 카보닐 및 PEG화된 카바메이트를 포함하는 치환된 아실 기를 포함하나 이들로 한정되지 않는다. N 말단에 대한 대표적인 캡핑 기는 하기 기들을 포함한다:
Figure pat00011
거대고리 또는 거대고리 형성 연결제와 함께 본원에서 사용된 바와 같이 용어 "구성원"은 거대고리를 형성하거나 형성할 수 있는 원자를 치징하고 치환기 또는 측쇄 원자를 배제한다. 유사하게, 사이클로데칸, 1,2-디플루오로-데칸 및 1,3-디메틸 사이클로데칸은 수소 또는 플루오로 치환기 또는 메틸 측쇄가 거대고리의 형성에 참여하지 않기 때문에 모두 10원 거대고리로서 간주된다.
분자 구조의 일부로서 사용될 때 부호 "
Figure pat00012
"는 단일 결합, 또는 트랜스 또는 시스 이중 결합을 지칭한다.
용어 "아미노산 측쇄"는 아미노산에서 α 탄소에 부착된 모이어티를 지칭한다. 예를 들면, 알라닌에 대한 아미노산 측쇄는 메틸이고, 페닐알라닌에 대한 아미노산 측쇄는 페닐메틸이고, 시스테인에 대한 아미노산 측쇄는 티오메틸이고, 아스파테이트에 대한 아미노산 측쇄는 카복시메틸이고, 티로신에 대한 아미노산 측쇄는 4-하이드록시페닐메틸이다. 다른 비천연 발생 아미노산 측쇄, 예를 들면, 자연에서 발생하는 비-천연 아미노산 측쇄(예를 들면, 아미노산 대사물질) 또는 합성적으로 제조된 비-천연 아미노산 측쇄(예를 들면, α,α-이치환된 아미노산)도 포함된다.
용어 "α,α-이치환된 아미노산"은 2개의 천연 또는 비천연 아미노산 측쇄에 부착된 탄소(α 탄소)에 결합된 아미노 기 및 카복실 기 둘 다를 함유하는 분자 또는 모이어티를 지칭한다.
용어 "폴리펩티드"는 공유결합(예를 들면, 아미드 결합)에 의해 연결된 2개 이상의 천연 또는 비천연 발생 아미노산을 포괄한다. 본원에 기재된 바와 같은 폴리펩티드는 전장 단백질(예를 들면, 전체적으로 프로세싱된 단백질)뿐만 아니라 보다 짧은 아미노산 서열(예를 들면, 천연 발생 단백질의 단편 또는 합성 폴리펩티드 단편)도 포함한다.
본원에서 사용된 바와 같이, 용어 "거대고리화 시약" 또는 "거대고리 형성 시약"은 2개의 반응성 기들 사이의 반응을 매개함으로써 본 발명의 펩티도미메틱 거대고리를 제조하는 데에 사용될 수 있는 임의의 시약을 지칭한다. 반응성 기는 예를 들면, 아지드 및 알킨일 수 있고, 이 경우 거대고리화 시약은 Cu 시약, 예컨대, 반응성 Cu(I) 종, 예컨대, CuBr, CuI 또는 CuOTf를 제공하는 시약뿐만 아니라, 환원제, 예컨대, 아스코르브산 또는 아스코르브산나트륨의 첨가에 의해 동일반응계(in situ)에서 활성 Cu(I) 시약으로 전환될 수 있는 Cu(II) 염, 예컨대, Cu(CO2CH3)2, CuSO4 및 CuCl2도 포함하나 이들로 한정되지 않는다. 거대고리화 시약은 예를 들면, 당업계에 공지된 Ru 시약, 예컨대, Cp*RuCl(PPh3)2, [Cp*RuCl]4, 또는 반응성 Ru(II) 종을 제공할 수 있는 다른 Ru 시약을 추가로 포함할 수 있다. 다른 경우, 반응성 기는 말단 올레핀이다. 이러한 실시양태에서, 거대고리화 시약 또는 거대고리 형성 시약은 안정화된 후전이금속 카벤 착물 촉매, 예컨대, VIII 족 전이금속 카벤 촉매를 포함하나 이들로 한정되지 않는 복분해 촉매이다. 예를 들면, 이러한 촉매는 +2 산화 상태, 16의 전자수 및 5배위 구조를 갖는 Ru 및 Os 금속 중심이다. 추가 촉매는 문헌(Grubbs et al., "Ring Closing Metathesis and Related Processes in Organic Synthesis" Acc. Chem. Res. 1995, 28, 446-452) 및 미국 특허 제5,811,515호에 개시되어 있다. 다른 경우, 반응성 기는 티올 기이다. 이러한 실시양태에서, 거대고리화 시약은 예를 들면, 2개의 티올 반응성 기, 예컨대, 할로겐 기를 작용기로서 갖는 연결제이다.
용어 "할로" 또는 "할로겐"은 불소, 염소, 브롬 또는 요오드, 또는 이들의 라디칼을 지칭한다.
용어 "알킬"은 표시된 수의 탄소 원자를 함유하는 직쇄 또는 분지쇄인 탄화수소 쇄를 지칭한다. 예를 들면, C1-C10은 기가 그의 내부에 1개 내지 10개(포함)의 탄소 원자를 갖는다는 것을 표시한다. 임의의 수치 표시가 없는 경우, "알킬"은 그의 내부에 1개 내지 20개(포함)의 탄소 원자를 갖는 쇄(직쇄 또는 분지쇄)이다.
용어 "알킬렌"은 2가 알킬(즉, -R-)을 지칭한다.
용어 "알케닐"은 하나 이상의 탄소-탄소 이중 결합을 갖는 직쇄 또는 분지쇄인 탄화수소 쇄를 지칭한다. 알케닐 모이어티는 표시된 수의 탄소 원자를 함유한다. 예를 들면, C2-C10은 기가 그의 내부에 2개 내지 10개(포함)의 탄소 원자를 갖는다는 것을 표시한다. 용어 "저급 알케닐"은 C2-C6 알케닐 쇄를 지칭한다. 임의의 수치 표시가 없는 경우, "알케닐"은 그의 내부에 2개 내지 20개(포함)의 탄소 원자를 갖는 쇄(직쇄 또는 분지쇄)이다.
용어 "알키닐"은 하나 이상의 탄소-탄소 삼중 결합을 갖는 직쇄 또는 분지쇄인 탄화수소 쇄를 지칭한다. 알키닐 모이어티는 표시된 수의 탄소 원자를 함유한다. 예를 들면, C2-C10은 기가 그의 내부에 2개 내지 10개(포함)의 탄소 원자를 갖는다는 것을 표시한다. 용어 "저급 알키닐"은 C2-C6 알키닐 쇄를 지칭한다. 임의의 수치 표시가 없는 경우, "알키닐"은 그의 내부에 2개 내지 20개(포함)의 탄소 원자를 갖는 쇄(직쇄 또는 분지쇄)이다.
용어 "아릴"은 6개의 탄소를 갖는 일환형 방향족 고리 시스템 또는 10개의 탄소를 갖는 이환형 방향족 고리 시스템을 지칭하고, 이때 각각의 고리의 0개, 1개, 2개, 3개 또는 4개의 원자는 치환기로 치환된다. 아릴 기의 예로는 페닐, 나프틸 등이 있다. 용어 "아릴알킬" 또는 용어 "아르알킬"은 아릴로 치환된 알킬을 지칭한다. 용어 "아릴알콕시"는 아릴로 치환된 알콕시를 지칭한다.
"아릴알킬"은 아릴 기의 수소 원자들 중 하나가 상기 정의된 바와 같은 C1-C5 알킬 기로 치환되어 있는, 상기 정의된 바와 같은 아릴 기를 지칭한다. 아릴알킬 기의 대표적인 예로는 2-메틸페닐, 3-메틸페닐, 4-메틸페닐, 2-에틸페닐, 3-에틸페닐, 4-에틸페닐, 2-프로필페닐, 3-프로필페닐, 4-프로필페닐, 2-부틸페닐, 3-부틸페닐, 4-부틸페닐, 2-펜틸페닐, 3-펜틸페닐, 4-펜틸페닐, 2-이소프로필페닐, 3-이소프로필페닐, 4-이소프로필페닐, 2-이소부틸페닐, 3-이소부틸페닐, 4-이소부틸페닐, 2-sec-부틸페닐, 3-sec-부틸페닐, 4-sec-부틸페닐, 2-t-부틸페닐, 3-t-부틸페닐 및 4-t-부틸페닐이 있으나 이들로 한정되지 않는다.
"아릴아미도"는 아릴 기의 수소 원자들 중 하나가 하나 이상의 -C(O)NH2 기로 치환되어 있는, 상기 정의된 바와 같은 아릴 기를 지칭한다. 아릴아미도 기의 대표적인 예로는 2-C(O)NH2-페닐, 3-C(O)NH2-페닐, 4-C(O)NH2-페닐, 2-C(O)NH2-피리딜, 3-C(O)NH2-피리딜 및 4-C(O)NH2-피리딜이 있다.
"알킬헤테로사이클"은 C1-C5 알킬 기의 수소 원자들 중 하나가 헤테로사이클로 치환되어 있는, 상기 정의된 바와 같은 C1-C5 알킬 기를 지칭한다. 알킬헤테로사이클 기의 대표적인 예로는 -CH2CH2-모폴린, -CH2CH2-피페리딘, -CH2CH2CH2-모폴린 및 -CH2CH2CH2-이미다졸이 있으나 이들로 한정되지 않는다.
"알킬아미도"는 C1-C5 알킬 기의 수소 원자들 중 하나가 -C(O)NH2로 치환되어 있는, 상기 정의된 바와 같은 C1-C5 알킬 기를 지칭한다. 알킬아미도 기의 대표적인 예로는 -CH2-C(O)NH2, -CH2CH2-C(O)NH2, -CH2CH2CH2C(O)NH2, -CH2CH2CH2CH2C(O)NH2, -CH2CH2CH2CH2CH2C(O)NH2, -CH2CH(C(O)NH2)CH3, -CH2CH(C(O)NH2)CH2CH3, -CH(C(O)NH2)CH2CH3, -C(CH3)2CH2C(O)NH2, -CH2-CH2-NH-C(O)-CH3, -CH2-CH2-NH-C(O)-CH3-CH3 및 -CH2-CH2-NH-C(O)-CH=CH2가 있으나 이들로 한정되지 않는다.
"알칸올"은 C1-C5 알킬 기의 수소 원자들 중 하나가 하이드록실 기로 치환되어 있는, 상기 정의된 바와 같은 C1-C5 알킬 기를 지칭한다. 알칸올 기의 대표적인 예로는 -CH2OH, -CH2CH2OH, -CH2CH2CH2OH, -CH2CH2CH2CH2OH, -CH2CH2CH2CH2CH2OH, -CH2CH(OH)CH3, -CH2CH(OH)CH2CH3, -CH(OH)CH3 및 -C(CH3)2CH2OH가 있으나 이들로 한정되지 않는다.
"알킬카복시"는 C1-C5 알킬 기의 수소 원자들 중 하나가 --COOH 기로 치환되어 있는, 상기 정의된 바와 같은 C1-C5 알킬 기를 지칭한다. 알킬카복시 기의 대표적인 예로는 -CH2COOH, -CH2CH2COOH, -CH2CH2CH2COOH, -CH2CH2CH2CH2COOH, -CH2CH(COOH)CH3, -CH2CH2CH2CH2CH2COOH, -CH2CH(COOH)CH2CH3, -CH(COOH)CH2CH3 및 -C(CH3)2CH2COOH가 있으나 이들로 한정되지 않는다.
본원에서 사용된 바와 같이, 용어 "사이클로알킬"은 3개 내지 12개의 탄소, 바람직하게는 3개 내지 8개의 탄소, 보다 바람직하게는 3개 내지 6개의 탄소를 갖는 포화된 및 부분적으로 불포화된 환형 탄화수소 기를 포함하고, 이때 사이클로알킬 기는 추가로 임의적으로 치환된다. 몇몇 사이클로알킬 기는 사이클로프로필, 사이클로부틸, 사이클로펜틸, 사이클로펜테닐, 사이클로헥실, 사이클로헥세닐, 사이클로헵틸 및 사이클로옥틸을 포함하나 이들로 한정되지 않는다.
용어 "헤테로아릴"은 단환형인 경우 1개 내지 3개의 헤테로원자, 이환형인 경우 1개 내지 6개의 헤테로원자 또는 삼환형인 경우 1개 내지 9개의 헤테로원자를 갖는 방향족 5원 내지 8원 단환형, 8원 내지 12원 이환형 또는 11원 내지 14원 삼환형 고리 시스템을 지칭하고, 이때 상기 헤테로원자는 O, N 및 S로부터 선택되고(예를 들면, 단환형, 이환형 또는 삼환형인 경우 각각 탄소 원자 및 1개 내지 3개, 1개 내지 6개 또는 1개 내지 9개의 O, N 또는 S 헤테로원자), 각각의 고리의 0개, 1개, 2개, 3개 또는 4개 원자는 치환기로 치환된다. 헤테로아릴 기의 예로는 피리딜, 푸릴 또는 푸라닐, 이미다졸릴, 벤즈이미다졸릴, 피리미디닐, 티오페닐 또는 티에닐, 퀴놀리닐, 인돌릴, 티아졸릴 등이 있다.
용어 "헤테로아릴알킬" 또는 용어 "헤테로아르알킬"은 헤테로아릴로 치환된 알킬을 지칭한다. 용어 "헤테로아릴알콕시"는 헤테로아릴로 치환된 알콕시를 지칭한다.
용어 "헤테로아릴알킬" 또는 용어 "헤테로아르알킬"은 헤테로아릴로 치환된 알킬을 지칭한다. 용어 "헤테로아릴알콕시"는 헤테로아릴로 치환된 알콕시를 지칭한다.
용어 "헤테로사이클릴"은 단환형인 경우 1개 내지 3개의 헤테로원자, 이환형인 경우 1개 내지 6개의 헤테로원자 또는 삼환형인 경우 1개 내지 9개의 헤테로원자를 갖는 비방향족 5원 내지 8원 단환형, 8원 내지 12원 이환형 또는 11원 내지 14원 삼환형 고리 시스템을 지칭하고, 이때 상기 헤테로원자는 O, N 및 S로부터 선택되고(예를 들면, 단환형, 이환형 또는 삼환형인 경우 각각 탄소 원자 및 1개 내지 3개, 1개 내지 6개 또는 1개 내지 9개의 O, N 또는 S 헤테로원자), 각각의 고리의 0개, 1개, 2개 또는 3개 원자는 치환기로 치환된다. 헤테로사이클릴 기의 예로는 피페라지닐, 피롤리디닐, 디옥사닐, 모폴리닐, 테트라하이드로푸라닐 등이 있다.
용어 "치환기"는 임의의 분자, 화합물 또는 모이어티 상의 제2 원자 또는 기, 예컨대, 수소 원자를 치환시키는 기를 지칭한다. 적합한 치환기는 할로, 하이드록시, 머캡토, 옥소, 니트로, 할로알킬, 알킬, 알크아릴, 아릴, 아르알킬, 알콕시, 티오알콕시, 아릴옥시, 아미노, 알콕시카보닐, 아미도, 카복시, 알칸설포닐, 알킬카보닐 및 시아노 기를 포함하나 이들로 한정되지 않는다.
몇몇 실시양태에서, 본 발명의 화합물은 하나 이상의 비대칭 중심을 함유하므로 라세미체 및 라세미체 혼합물, 단일 거울상이성질체, 개별 부분입체이성질체 및 부분입체이성질체 혼합물로서 존재한다. 이들 화합물의 모든 이러한 이성질체 형태들은 명확히 달리 제공되어 있지 않은 한 본 발명에 포함된다. 몇몇 실시양태에서, 본 발명의 화합물은 다수의 호변이성질체 형태들로도 표시되고, 이 경우 본 발명은 본원에 기재된 화합물의 모든 호변이성질체 형태들을 포함한다(예를 들면, 고리 시스템의 알킬화가 다수의 부위들에서 알킬화를 발생시키는 경우, 본 발명은 모든 이러한 반응 생성물들을 포함함). 이러한 화합물의 모든 이러한 이성질체 형태들은 명확히 달리 제공되어 있지 않은 한 본 발명에 포함된다. 본원에 기재된 화합물의 모든 결정 형태들은 명확히 달리 제공되어 있지 않은 한 본 발명에 포함된다.
본원에서 사용된 바와 같이, 용어 "증가시킨다" 및 "감소시킨다"는 각각 5% 이상의 통계적으로 유의한(즉, p < 0.1) 증가 또는 감소를 야기한다는 것을 의미한다.
본원에서 사용된 바와 같이, 변수에 대한 수치 범위의 언급은 그 범위 내의 임의의 값과 동등한 변수를 사용하여 본 발명을 실시할 수 있다는 것을 의미하기 위한 것이다. 따라서, 본질적으로 불연속적인 변수의 경우, 상기 변수는 수치 범위의 종점을 포함하는, 상기 수치 범위 내의 임의의 정수 값과 동등하다. 유사하게, 본질적으로 연속적인 변수의 경우, 상기 변수는 수치 범위의 종점을 포함하는, 상기 수치 범위 내의 임의의 실제 값과 동등하다. 한 예로서(그러나 이로 한정되지 않음), 0 내지 2 사이의 값을 갖는다고 기재된 변수는 이 변수가 본질적으로 불연속적인 경우 값 0, 1 또는 2를 갖고, 상기 변수가 본질적으로 연속적인 경우 값 0.0, 0.1, 0.01, 0.001, 또는 0 이상 내지 2 이하의 임의의 다른 실수 값을 갖는다.
본원에서 사용된 바와 같이, 달리 구체적으로 표시되지 않은 한, 단어 "또는"은 "및/또는"의 포괄적 의미로 사용되고 "어느 하나/또는"의 배제적 의미로 사용되지 않는다.
용어 "평균적으로"는 각각의 데이터 점에 대해 3회 이상의 독립적 반복실험을 수행함으로써 유도된 평균 값을 나타낸다.
용어 "생물학적 활성"은 본 발명의 거대고리의 구조적 및 기능적 성질을 포괄한다. 생물학적 활성은 예를 들면, 구조적 안정성, α 나선도, 표적에 대한 친화성, 단백질용해성 분해에 대한 내성, 세포 투과능, 세포내 안정성, 생체내 안정성, 또는 이들의 임의의 조합이다.
본 발명의 하나 이상의 구체적인 실시양태의 세부사항은 첨부된 도면 및 하기 설명에 기재되어 있다. 본 발명의 다른 특징, 목적 및 장점은 설명 및 도면, 및 특허청구범위로부터 자명해질 것이다.
몇몇 실시양태에서, 펩티드 서열은 p53 단백질로부터 유도된다.
본 발명에서 사용되기에 적합한 p53 펩티드의 비한정적 예시적 목록은 이하에 제시되어 있다:
[표 1]
Figure pat00013
[표 2]
Figure pat00014
[표 3]
Figure pat00015
표 3 및 다른 부분에서, "Aib"는 2-아미노이소부티르산 잔기를 나타내는 반면, "Ac3c"는 아미노사이클로프로판 카복실산 잔기를 나타낸다.
펩티도미메틱 거대고리
몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 하기 화학식 I로 표시된다:
[화학식 I]
Figure pat00016
상기 식에서,
A, C, D 및 E는 각각 독립적으로 천연 또는 비천연 아미노산이고, 말단 D 및 E는 독립적으로 임의적으로 캡핑 기를 포함하고;
B는 천연 또는 비천연 아미노산, 아미노산 유사체,
Figure pat00017
[-NH-L3-CO-], [-NH-L3-SO2-] 또는 [-NH-L3-]이고;
R1 및 R2는 독립적으로 비치환되거나 할로로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 사이클로알킬알킬, 헤테로알킬, 헤테로사이클로알킬 또는 -H이고;
R3은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 헤테로알킬, 사이클로알킬, 헤테로사이클로알킬, 사이클로알킬알킬, 사이클로아릴, 헤테로사이클로아릴 또는 수소이고;
L은 화학식 -L1-L2-의 거대고리 형성 연결제이고;
L1 및 L2는 독립적으로 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 사이클로아릴렌, 헤테로사이클로아릴렌 또는 [-R4-K-R4-]n이고, 이때 이들 각각은 임의적으로 R5로 치환되고;
R4는 각각 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 아릴렌 또는 헤테로아릴렌이고;
K는 각각 O, S, SO, SO2, CO, CO2 또는 CONR3이고;
R5는 각각 독립적으로 할로겐, 알킬, -OR6, -N(R6)2, -SR6, -SOR6, -SO2R6, -CO2R6, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R6은 각각 독립적으로 -H, 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R7은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이거나, D 잔기를 갖는 환형 구조의 일부이고;
R8은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이거나, E 잔기를 갖는 환형 구조의 일부이고;
v 및 w는 독립적으로 1 내지 1000의 정수이고;
u는 1 내지 10의 정수이고;
x, y 및 z는 독립적으로 0 내지 10의 정수이고;
n은 1 내지 5의 정수이다.
한 실시양태에서, L1 및 L2는 단독으로 또는 조합으로 트리아졸 또는 티오에테르를 형성하지 않는다.
한 예에서, R1 및 R2 중 하나 이상은 비치환되거나 할로로 치환된 알킬이다. 또 다른 예에서, R1 및 R2 둘 다 독립적으로 비치환되거나 할로로 치환된 알킬이다. 몇몇 실시양태에서, R1 및 R2 중 하나 이상은 메틸이다. 다른 실시양태에서, R1 및 R2는 메틸이다.
본 발명의 몇몇 실시양태에서, x+y+z는 3 이상이다. 본 발명의 다른 실시양태에서, x+y+z는 1, 2, 3, 4, 5, 6, 7, 8, 9 또는 10이다. 본 발명의 거대고리 또는 거대고리 전구체에서 A, B, C, D 또는 E의 각각의 발생은 독립적으로 선택된다. 예를 들면, x가 3인 경우 화학식 [A]x로 표시된 서열은 아미노산이 동일하지 않은 실시양태, 예를 들면, Gln-Asp-Ala뿐만 아니라, 아미노산이 동일한 실시양태, 예를 들면, Gln-Gln-Gln을 포괄한다. 이것은 표시된 범위 내에서 x, y 또는 z의 임의의 값에 적용된다. 유사하게, u가 1을 초과하는 경우, 본 발명의 각각의 화합물은 동일하거나 상이한 펩티도미메틱 거대고리를 포괄할 수 있다. 예를 들면, 본 발명의 화합물은 상이한 연결제 길이 또는 화학적 조성을 포함하는 펩티도미메틱 거대고리를 포함할 수 있다.
몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 R8이 나선내 수소결합을 가능하게 하는 -H인, α 나선인 2차 구조를 포함한다. 몇몇 실시양태에서, A, B, C, D 및 E 중 하나 이상은 α,α-이치환된 아미노산이다. 한 예에서, B는 α,α-이치환된 아미노산이다. 예를 들면, A, B, C, D 및 E 중 하나 이상은 2-아미노이소부티르산이다. 다른 실시양태에서, A, B, C, D 및 E 중 하나 이상은
Figure pat00018
이다.
다른 실시양태에서, 제1 Cα부터 제2 Cα까지 측정된 거대고리 형성 연결제 L의 길이는 원하는 2차 펩티드 구조, 예컨대, 제1 Cα와 제2 Cα 사이의 잔기들을 포함하나 반드시 이들로 한정되지 않는 펩티도미메틱 거대고리의 잔기들에 의해 형성된 α 나선을 안정화시키도록 선택된다.
한 실시양태에서, 화학식 I의 펩티도미메틱 거대고리는 다음과 같다:
Figure pat00019
상기 식에서,
R1 및 R2는 각각 독립적으로 비치환되거나 할로로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 사이클로알킬알킬, 헤테로알킬, 헤테로사이클로알킬 또는 -H이다.
관련 실시양태에서, 화학식 I의 펩티도미메틱 거대고리는 다음과 같다:
Figure pat00020
Figure pat00021
다른 실시양태에서, 화학식 I의 펩티도미메틱 거대고리는 이하에 제시된 화학식들 중 임의의 화학식의 화합물이다:
Figure pat00022
Figure pat00023
상기 식에서,
"AA"는 임의의 천연 또는 비천연 아미노산 측쇄를 나타내고, "
Figure pat00024
"는 상기 정의된 바와 같은 [D]v 또는 [E]w이고, n은 0 내지 20, 50, 100, 200, 300, 400 또는 500의 정수이다. 몇몇 실시양태에서, n은 0이다. 다른 실시양태에서, n은 50 미만이다.
거대고리 형성 연결제 L의 예시적 실시양태는 이하에 제시되어 있다:
Figure pat00025
다른 실시양태에서, 화학식 I의 화합물에서 D 및/또는 E는 세포 섭취(uptake)를 용이하게 하기 위해 추가로 변형된다. 몇몇 실시양태에서, 펩티도미메틱 거대고리의 지질화(lipidating) 또는 PEG화(PEGylating)는 세포 섭취를 용이하게 하고, 생체이용성을 증가시키고, 혈액 순환을 증가시키고, 약동학을 변경하고, 면역원성을 감소시키고/시키거나 필요한 투여 빈도를 감소시킨다.
다른 실시양태에서, 화학식 I의 화합물에서 [D] 및 [E] 중 하나 이상은 펩티도미메틱 거대고리가 2개 이상의 거대고리 형성 연결제를 포함하도록 추가 거대고리 형성 연결제를 포함하는 모이어티를 나타낸다. 특정 실시양태에서, 펩티도미메틱 거대고리는 2개의 거대고리 형성 연결제를 포함한다. 한 실시양태에서, u는 2이다.
본 발명의 펩티도미메틱 거대고리에서, 본원에 기재된 거대고리 형성 연결제들 중 임의의 거대고리 형성 연결제는 표 1 내지 4에 나타낸 서열들 중 임의의 서열 및 본원에서 표시된 R- 치환기들 중 임의의 R- 치환기와 임의의 조합으로 사용될 수 있다.
몇몇 실시양태에서, 펩티도미메틱 거대고리는 하나 이상의 α 나선 모티프를 포함한다. 예를 들면, 화학식 I의 화합물에서 A, B 및/또는 C는 하나 이상의 α 나선을 포함한다. 일반적인 문제로서, α 나선은 회전 당 3개 내지 4개의 아미노산 잔기를 포함한다. 몇몇 실시양태에서, 펩티도미메틱 거대고리의 α 나선은 1개 내지 5개의 회전을 포함하므로 3개 내지 20개의 아미노산 잔기를 포함한다. 특정 실시양태에서, α 나선은 1개의 회전, 2개의 회전, 3개의 회전, 4개의 회전 또는 5개의 회전을 포함한다. 몇몇 실시양태에서, 거대고리 형성 연결제는 펩티도미메틱 거대고리 내에 포함된 α 나선 모티프를 안정화시킨다. 따라서, 몇몇 실시양태에서, 제1 Cα부터 제2 Cα까지 측정된 거대고리 형성 연결제 L의 길이는 α 나선의 안정성을 증가시키도록 선택된다. 몇몇 실시양태에서, 거대고리 형성 연결제는 α 나선의 1개 회전 내지 5개 회전에 걸쳐 있다. 몇몇 실시양태에서, 거대고리 형성 연결제는 α 나선의 대략 1개 회전, 2개 회전, 3개 회전, 4개 회전 또는 5개 회전에 걸쳐 있다. 몇몇 실시양태에서, 거대고리 형성 연결제의 길이는 α 나선의 회전 당 대략 5Å 내지 9Å, 또는 α 나선의 회전 당 대략 6Å 내지 8Å이다. 거대고리 형성 연결제가 α 나선의 대략 1개 회전에 걸쳐 있는 경우, 길이는 대략 5개의 탄소-탄소 결합 내지 13개의 탄소-탄소 결합, 대략 7개의 탄소-탄소 결합 내지 11개의 탄소-탄소 결합, 또는 대략 9개의 탄소-탄소 결합과 동등하다. 거대고리 형성 연결제가 α 나선의 대략 2개 회전에 걸쳐 있는 경우, 길이는 대략 8개의 탄소-탄소 결합 내지 16개의 탄소-탄소 결합, 대략 10개의 탄소-탄소 결합 내지 14개의 탄소-탄소 결합, 또는 대략 12개의 탄소-탄소 결합과 동등하다. 거대고리 형성 연결제가 α 나선의 대략 3개 회전에 걸쳐 있는 경우, 길이는 대략 14개의 탄소-탄소 결합 내지 22개의 탄소-탄소 결합, 대략 16개의 탄소-탄소 결합 내지 20개의 탄소-탄소 결합, 또는 대략 18개의 탄소-탄소 결합과 동등하다. 거대고리 형성 연결제가 α 나선의 대략 4개 회전에 걸쳐 있는 경우, 길이는 대략 20개의 탄소-탄소 결합 내지 28개의 탄소-탄소 결합, 대략 22개의 탄소-탄소 결합 내지 26개의 탄소-탄소 결합, 또는 대략 24개의 탄소-탄소 결합과 동등하다. 거대고리 형성 연결제가 α 나선의 대략 5개 회전에 걸쳐 있는 경우, 길이는 대략 26개의 탄소-탄소 결합 내지 34개의 탄소-탄소 결합, 대략 28개의 탄소-탄소 결합 내지 32개의 탄소-탄소 결합, 또는 대략 30개의 탄소-탄소 결합과 동등하다. 거대고리 형성 연결제가 α 나선의 대략 1개 회전에 걸쳐 있는 경우, 연결은 대략 4개의 원자 내지 12개의 원자, 대략 6개의 원자 내지 10개의 원자, 또는 대략 8개의 원자를 함유한다. 거대고리 형성 연결제가 α 나선의 대략 2개 회전에 걸쳐 있는 경우, 연결은 대략 7개의 원자 내지 15개의 원자, 대략 9개의 원자 내지 13개의 원자, 또는 대략 11개의 원자를 함유한다. 거대고리 형성 연결제가 α 나선의 대략 3개 회전에 걸쳐 있는 경우, 연결은 대략 13개의 원자 내지 21개의 원자, 대략 15개의 원자 내지 19개의 원자, 또는 대략 17개의 원자를 함유한다. 거대고리 형성 연결제가 α 나선의 대략 4개 회전에 걸쳐 있는 경우, 연결은 대략 19개의 원자 내지 27개의 원자, 대략 21개의 원자 내지 25개의 원자, 또는 대략 23개의 원자를 함유한다. 거대고리 형성 연결제가 α 나선의 대략 5개 회전에 걸쳐 있는 경우, 연결은 대략 25개의 원자 내지 33개의 원자, 대략 27개의 원자 내지 31개의 원자, 또는 대략 29개의 원자를 함유한다. 거대고리 형성 연결제가 α 나선의 대략 1개 회전에 걸쳐 있는 경우, 생성된 거대고리는 대략 17개의 구성원 내지 25개의 구성원, 대략 19개의 구성원 내지 23개의 구성원, 또는 대략 21개의 구성원을 함유하는 고리를 형성한다. 거대고리 형성 연결제가 α 나선의 대략 2개 회전에 걸쳐 있는 경우, 생성된 거대고리는 대략 29개의 구성원 내지 37개의 구성원, 대략 31개의 구성원 내지 35개의 구성원, 또는 대략 33개의 구성원을 함유하는 고리를 형성한다. 거대고리 형성 연결제가 α 나선의 대략 3개 회전에 걸쳐 있는 경우, 생성된 거대고리는 대략 44개의 구성원 내지 52개의 구성원, 대략 46개의 구성원 내지 50개의 구성원, 또는 대략 48개의 구성원을 함유하는 고리를 형성한다. 거대고리 형성 연결제가 α 나선의 대략 4개 회전에 걸쳐 있는 경우, 생성된 거대고리는 대략 59개의 구성원 내지 67개의 구성원, 대략 61개의 구성원 내지 65개의 구성원, 또는 대략 63개의 구성원을 함유하는 고리를 형성한다. 거대고리 형성 연결제가 α 나선의 대략 5개 회전에 걸쳐 있는 경우, 생성된 거대고리는 대략 74개의 구성원 내지 82개의 구성원, 대략 76개의 구성원 내지 80개의 구성원, 또는 대략 78개의 구성원을 함유하는 고리를 형성한다.
다른 실시양태에서, 본 발명은 하기 화학식 IV 또는 IVa의 펩티도미메틱 거대고리를 제공한다:
[화학식 IV]
Figure pat00026
[화학식 IVa]
Figure pat00027
상기 식에서,
A, C, D 및 E는 각각 독립적으로 천연 또는 비천연 아미노산이고, 말단 D 및 E는 독립적으로 임의적으로 캡핑 기를 포함하고;
B는 천연 또는 비천연 아미노산, 아미노산 유사체,
Figure pat00028
[-NH-L3-CO-], [-NH-L3-SO2-] 또는 [-NH-L3-]이고;
R1 및 R2는 독립적으로 비치환되거나 할로로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 사이클로알킬알킬, 헤테로알킬, 헤테로사이클로알킬 또는 -H이거나, E 잔기를 갖는 환형 구조의 일부이고;
R3은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 헤테로알킬, 사이클로알킬, 헤테로사이클로알킬, 사이클로알킬알킬, 사이클로아릴, 헤테로사이클로아릴 또는 수소이고;
L은 화학식 -L1-L2-의 거대고리 형성 연결제이고;
L1 및 L2는 독립적으로 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 사이클로아릴렌, 헤테로사이클로아릴렌 또는 [-R4-K-R4-]n이고, 이때 이들 각각은 임의적으로 R5로 치환되고;
R4는 각각 알킬렌, 알케닐렌, 알키닐렌, 헤테로알킬렌, 사이클로알킬렌, 헤테로사이클로알킬렌, 아릴렌 또는 헤테로아릴렌이고;
K는 각각 O, S, SO, SO2, CO, CO2 또는 CONR3이고;
R5는 각각 독립적으로 할로겐, 알킬, -OR6, -N(R6)2, -SR6, -SOR6, -SO2R6, -CO2R6, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R6은 각각 독립적으로 -H, 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 형광 모이어티, 방사성 동위원소 또는 치료제이고;
R7은 임의적으로 R5로 치환된 알킬, 알케닐, 알키닐, 아릴알킬, 사이클로알킬, 헤테로알킬, 사이클로알킬알킬, 헤테로사이클로알킬, 사이클로아릴, 헤테로사이클로아릴 또는 -H이고;
v 및 w는 독립적으로 1 내지 1000의 정수이고;
u는 1 내지 10의 정수이고;
x, y 및 z는 독립적으로 0 내지 10의 정수이고;
n은 1 내지 5의 정수이다.
한 예에서, L1 및 L2는 단독으로 또는 조합으로 트리아졸 또는 티오에테르를 형성하지 않는다.
한 예에서, R1 및 R2 중 하나 이상은 비치환되거나 할로로 치환된 알킬이다. 또 다른 예에서, R1 및 R2 둘 다 독립적으로 비치환되거나 할로로 치환된 알킬이다. 몇몇 실시양태에서, R1 및 R2 중 하나 이상은 메틸이다. 다른 실시양태에서, R1 및 R2는 메틸이다.
본 발명의 몇몇 실시양태에서, x+y+z는 1 이상이다. 본 발명의 다른 실시양태에서, x+y+z는 2 이상이다. 본 발명의 다른 실시양태에서, x+y+z는 1, 2, 3, 4, 5, 6, 7, 8, 9 또는 10이다. 본 발명의 거대고리 또는 거대고리 전구체에서 A, B, C, D 또는 E의 각각의 발생은 독립적으로 선택된다. 예를 들면, x가 3인 경우 화학식 [A]x로 표시된 서열은 아미노산이 동일하지 않은 실시양태, 예를 들면, Gln-Asp-Ala뿐만 아니라, 아미노산이 동일한 실시양태, 예를 들면, Gln-Gln-Gln을 포괄한다. 이것은 표시된 범위 내에서 x, y 또는 z의 임의의 값에 적용된다.
몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 R8이 나선내 수소결합을 가능하게 하는 -H인, α 나선인 2차 구조를 포함한다. 몇몇 실시양태에서, A, B, C, D 및 E 중 하나 이상은 α,α-이치환된 아미노산이다. 한 예에서, B는 α,α-이치환된 아미노산이다. 예를 들면, A, B, C, D 및 E 중 하나 이상은 2-아미노이소부티르산이다. 다른 실시양태에서, A, B, C, D 및 E 중 하나 이상은
Figure pat00029
이다.
다른 실시양태에서, 제1 Cα부터 제2 Cα까지 측정된 거대고리 형성 연결제 L의 길이는 원하는 2차 펩티드 구조, 예컨대, 제1 Cα와 제2 Cα 사이의 잔기들을 포함하나 반드시 이들로 한정되지 않는 펩티도미메틱 거대고리의 잔기들에 의해 형성된 α 나선을 안정화시키도록 선택된다.
거대고리 형성 연결제 L의 예시적 실시양태는 이하에 제시되어 있다:
Figure pat00030
펩티도미메틱 거대고리의 제조
본 발명의 펩티도미메틱 거대고리는 당업계에 공지된 다양한 방법들 중 임의의 방법에 의해 제조될 수 있다. 예를 들면, 표 1, 2, 3 또는 4에서 "X"로 표시된 잔기들 중 임의의 잔기는 동일한 분자 내의 제2 잔기와 가교제를 형성할 수 있는 잔기 또는 이러한 잔기의 전구체로 치환될 수 있다.
펩티도미메틱 거대고리의 형성을 수행하는 다양한 방법들이 당업계에 공지되어 있다. 예를 들면, 화학식 I의 펩티도미메틱 거대고리의 제조는 문헌(Schafmeister et al., J. Am. Chem. Soc. 122:5891-5892 (2000)); 문헌(Schafmeister & Verdine, J. Am. Chem. Soc. 122:5891 (2005)); 문헌(Walensky et al., Science 305:1466-1470 (2004)), 미국 특허 제7,192,713호 및 PCT 특허출원 공보 제WO 2008/121767호에 기재되어 있다. 인용된 참고문헌들에 개시된 α,α-이치환된 아미노산 및 아미노산 전구체는 펩티도미메틱 거대고리 전구체 폴리펩티드의 합성에서 사용될 수 있다. 예를 들면, "S5-올레핀 아미노산"은 (S)-α-(2'-펜테닐) 알라닌이고, "R8 올레핀 아미노산"은 (R)-α-(2'-옥테닐) 알라닌이다. 이러한 아미노산이 전구체 폴리펩티드 내로 도입된 후, 말단 올레핀은 복분해 촉매와 반응하여 펩티도미메틱 거대고리의 형성을 유발한다. 다양한 실시양태들에서, 하기 아미노산들이 펩티도미메틱 거대고리의 합성에서 사용될 수 있다:
Figure pat00031
다른 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 화학식 IV 또는 IVa의 펩티도미메틱 거대고리이다. 이러한 거대고리의 제조 방법은 예를 들면, 미국 특허 제7,202,332호에 기재되어 있다.
본 발명을 수행하기에 적합한 것으로서 예상되는, 펩티도미메틱 거대고리를 형성하는 추가 방법은 문헌(Mustapa, M. Firouz Mohd et al., J. Org. Chem (2003), 68, pp. 8193-8198), 문헌(Yang, Bin et al. Bioorg Med. Chem. Lett. (2004), 14, pp. 1403-1406), 미국 특허 제5,364,851호, 미국 특허 제5,446,128호, 미국 특허 제5,824,483호, 미국 특허 제6,713,280호 및 미국 특허 제7,202,332호에 개시된 방법들을 포함한다. 이러한 실시양태에서, α 위치에서 추가 치환기 R-을 함유하는 아미노산 전구체가 사용된다. 이러한 아미노산은 가교제가 치환되는 위치일 수 있는 원하는 위치, 또는 대안적으로 거대고리 전구체의 서열 내의 임의의 위치에서 거대고리 전구체 내로 도입된다. 그 후, 상기 전구체의 고리화가 기재된 방법에 따라 수행된다.
분석
본 발명의 펩티도미메틱 거대고리의 성질은 예를 들면, 이하에 기재된 방법을 이용함으로써 분석된다. 몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 본원에 기재된 치환기를 결여하는 상응하는 폴리펩티드에 비해 상대적으로 개선된 생물학적 성질을 갖는다.
α 나선도를 측정하기 위한 분석
용액에서, α 나선 도메인을 갖는 폴리펩티드의 2차 구조는 종종 "% 나선도"로 표현되는, 랜덤 코일(random coil) 구조와 α 나선 구조 사이의 동력학적 평형에 도달할 것이다. 따라서, 예를 들면, α 나선 도메인은 통상적으로 25% 미만의 α 나선 함량을 가지면서 용액에서 주로 랜덤 코일이다. 다른 한편으로, 최적화된 연결제를 갖는 펩티도미메틱 거대고리는 예를 들면, 상응하는 비가교결합된 폴리펩티드의 α 나선도보다 2배 이상 더 큰 α 나선도를 보유한다. 몇몇 실시양태에서, 본 발명의 거대고리는 50% 초과의 α 나선도를 보유할 것이다. 본 발명의 펩티도미메틱 거대고리의 나선도를 분석하기 위해, 화합물을 수용액(예를 들면, pH 7의 50 mM 인산칼륨 용액 또는 증류된 H2O, 25 μM 내지 50 μM의 농도)에 용해시킨다. 표준 측정 파라미터(예를 들면, 온도: 20℃; 파장: 190 nm 내지 260 nm; 단계 해상도: 0.5 nm; 속도: 20 nm/초; 축적: 10; 반응: 1초; 밴드 폭: 1 nm; 경로 길이: 0.1 cm)를 이용하여 분광편광계(예를 들면, 자스코(Jasco) J-710) 상에서 원이색법(CD) 스펙트럼을 수득한다. 평균 잔기 타원율(ellipticity)(예를 들면, [Φ]222실측치)을 모델 나선 데카펩티드에 대해 보고된 값(Yang et al. (1986), Methods Enzymol. 130:208)으로 나눔으로써 각각의 펩티드의 α 나선 함량을 계산한다.
용융 온도( Tm )를 측정하기 위한 분석
2차 구조, 예컨대, α 나선을 포함하는 본 발명의 펩티도미메틱 거대고리는 예를 들면, 상응하는 비가교결합된 폴리펩티드보다 더 높은 용융 온도를 나타낸다. 전형적으로, 본 발명의 펩티도미메틱 거대고리는 수용액에서 고도로 안정한 구조를 표시하는 60℃ 초과의 Tm을 나타낸다. 용융 온도에 대한 거대고리 형성의 효과를 분석하기 위해, 펩티도미메틱 거대고리 또는 비변형된 펩티드를 증류된 H2O에 (예를 들면, 50 μM의 최종 농도로) 용해시키고, 표준 파라미터(예를 들면, 파장: 220 nm; 단계 해상도: 0.5 nm; 속도: 20 nm/초; 축적: 10; 반응: 1초; 밴드 폭: 1 nm; 온도 증가 속도: 1℃/분; 경로 길이: 0.1 cm)를 이용하여 분광편광계(예를 들면, 자스코 J-710) 상에서 일정한 온도 범위(예를 들면, 4℃ 내지 95℃)에 걸쳐 타원율에서의 변화를 측정함으로써 Tm을 측정한다.
프로테아제 내성 분석
펩티드 골격의 아미드 결합은 프로테아제에 의한 가수분해에 민감하므로 펩티드성 화합물이 생체내 신속한 분해에 의해 공격받기 쉽게 만든다. 그러나, 펩티드 나선 형성은 전형적으로 아미드 골격을 숨겨 단백질용해성 절단으로부터 상기 아미드 골격을 보호할 수 있다. 본 발명의 펩티도미메틱 거대고리를 시험관내 트립신 단백질용해로 처리하여 상응하는 비가교결합된 폴리펩티드와 비교된 분해 속도에서의 임의의 변화를 평가할 수 있다. 예를 들면, 펩티도미메틱 거대고리 및 상응하는 비가교결합된 폴리펩티드를 트립신 아가로스와 함께 인큐베이션하고, 다양한 시점에서 반응을 원심분리로 켄칭한 후, HPLC 주입을 수행하여 280 nm에서 자외선 흡수로 잔류 기질을 정량한다. 요약하건대, 펩티도미메틱 거대고리 및 펩티도미메틱 전구체(5 mcg)를 트립신 아가로스(피어스(Pierce))(S/E ~125)와 함께 0분, 10분, 20분, 90분 및 180분 동안 인큐베이션한다. 탁상 원심분리를 고속으로 수행하여 반응을 켄칭하고, 단리된 상청액 중의 남은 기질을 280 nm에서 HPLC에 기초한 피크 검출로 정량한다. 단백질용해성 반응은 1차 반응속도식을 나타내고, 속도 상수 k는 ln[S] 대 시간의 곡선으로부터 측정된다(k=-1X기울기).
생체외 안정성 분석
최적화된 연결제를 갖는 펩티도미메틱 거대고리는 예를 들면, 상응하는 비가교결합된 폴리펩티드의 생체외 수명보다 2배 이상 더 긴 생체외 수명을 보유하고 12시간 이상의 생체외 수명을 보유한다. 생체외 혈청 안정성 연구를 위해, 다양한 분석들이 이용될 수 있다. 예를 들면, 펩티도미메틱 거대고리 및 상응하는 비가교결합된 폴리펩티드(2 mcg)를 37℃에서 새로운 마우스, 래트 및/또는 인간 혈청(2 ㎖)과 0시간, 1시간, 2시간, 4시간, 8시간 및 24시간 동안 인큐베이션한다. 온전한 화합물의 수준을 측정하기 위해, 하기 절차를 이용할 수 있다: 100 ㎕의 혈청을 2 ㎖의 원심분리 튜브로 옮긴 후 10 ㎕의 50% 포름산 및 500 ㎕의 아세토니트릴을 첨가하고 4±2℃에서 14,000 RPM으로 10분 동안 원심분리함으로써 샘플을 추출한다. 그 다음, 상청액을 새로운 2 ㎖의 튜브로 옮기고 37℃에서 10 psi 미만의 N2 하에 터보뱁(Turbovap) 상에서 증발시킨다. 샘플을 100 ㎕의 50:50 아세토니트릴:물에서 재구성하고 LC-MS/MS 분석으로 처리한다.
시험관내 결합 분석
펩티도미메틱 거대고리 및 펩티도미메틱 전구체와 수용체 단백질의 결합 및 친화성을 평가하기 위해, 예를 들면, 형광 편광 분석(FPA)을 이용한다. FPA 기법은 편광된 광 및 형광 트레이서(tracer)를 사용하여 분자 배향 및 이동성을 측정한다. 편광된 광에 의해 여기되었을 때, 높은 겉보기 분자량을 갖는 분자에 부착된 형광 트레이서(예를 들면, FITC)(예를 들면, 큰 단백질에 결합된 FITC 표지된 펩티드)는 보다 작은 분자에 부착된 형광 트레이서(예를 들면, 용액에서 자유로운 FITC 표지된 펩티드)에 비해 그의 보다 느린 속도로 인해 보다 높은 수준의 편광된 형광을 방출한다.
예를 들면, 플루오레세인 표지된 펩티도미메틱 거대고리(25 nM)를 실온에서 결합 완충제(140 mM NaCl, 50 mM 트리스-HCL, pH 7.4)에서 수용체 단백질(25 nM 내지 1000 nM)과 함께 30분 동안 인큐베이션한다. 결합 활성을 예를 들면, 발광 분광계(예를 들면, 퍼킨-엘머(Perkin-Elmer) LS50B) 상에서의 형광 편광으로 측정한다. 예를 들면, 그래프패드 프리즘(Graphpad Prism) 소프트웨어(그래프패드 소프트웨어 인코포레이티드, 미국 캘리포니아주 샌디에고 소재)를 이용하는 비선형 회귀 분석으로 Kd 값을 측정할 수 있다. 본 발명의 펩티도미메틱 거대고리는 몇몇 경우 상응하는 비가교결합된 폴리펩티드와 유사하거나 상응하는 비가교결합된 폴리펩티드보다 낮은 Kd를 보인다.
펩티드-단백질 상호작용의 길항제를 특징규명하기 위한 시험관내 치환 분석
펩티드와 수용체 단백질 사이의 상호작용을 길항하는 화합물의 결합 및 친화성을 평가하기 위해, 예를 들면, 펩티도미메틱 전구체 서열로부터 유도된 플루오레세인 표지된 펩티도미메틱 거대고리를 사용하는 형광 편광 분석(FPA)을 이용한다. FPA 기법은 편광된 광 및 형광 트레이서를 사용하여 분자 배향 및 이동성을 측정한다. 편광된 광에 의해 여기되었을 때, 높은 겉보기 분자량을 갖는 분자에 부착된 형광 트레이서(예를 들면, FITC)(예를 들면, 큰 단백질에 결합된 FITC 표지된 펩티드)는 보다 작은 분자에 부착된 형광 트레이서(예를 들면, 용액에서 자유로운 FITC 표지된 펩티드)에 비해 그의 보다 느린 속도로 인해 보다 높은 수준의 편광된 형광을 방출한다. 플루오레세인 표지된 펩티도미메틱 거대고리와 수용체 단백질 사이의 상호작용을 길항하는 화합물은 경쟁 결합 FPA 실험에서 검출될 것이다.
예를 들면, 추정되는 길항제 화합물(1 nM 내지 1 mM) 및 플루오레세인 표지된 펩티도미메틱 거대고리(25 nM)를 실온에서 결합 완충제(140 mM NaCl, 50 mM 트리스-HCL, pH 7.4)에서 수용체 단백질(50 nM)과 함께 30분 동안 인큐베이션한다. 길항제 결합 활성을 예를 들면, 발광 분광계(예를 들면, 퍼킨-엘머 LS50B) 상에서의 형광 편광으로 측정한다. 예를 들면, 그래프패드 프리즘 소프트웨어(그래프패드 소프트웨어 인코포레이티드, 미국 캘리포니아주 샌디에고 소재)를 이용하는 비선형 회귀 분석으로 Kd 값을 측정할 수 있다.
임의의 부류의 분자, 예컨대, 유기 소분자, 펩티드, 올리고뉴클레오티드 또는 단백질은 이 분석에서 추정되는 길항제로서 조사될 수 있다.
친화성 선택-질량 분광측정에 의한 단백질-리간드 결합에 대한 분석
단백질에 대한 테스트 화합물의 결합 및 친화성을 평가하기 위해, 예를 들면, 친화성 선택-질량 분광측정 분석을 이용한다. 1 μM의 펩티도미메틱 거대고리 및 5 μM의 hMDM2를 사용하는 시스템 광범위 제어 실험에 대해 요약된 하기 대표적인 절차에 따라 단백질-리간드 결합 실험을 수행한다. 펩티도미메틱 거대고리의 40 μM 저장 용액의 1 ㎕ DMSO 분취액을 19 ㎕의 PBS(인산염 완충 식염수: 50 mM, pH 7.5, 150 mM NaCl을 함유하는 인산염 완충제)에 용해시킨다. 생성된 용액을 반복된 피펫팅으로 혼합하고 10,000 g에서 10분 동안 원심분리하여 정화한다. 생성된 상청액의 4 ㎕ 분취액에 4 ㎕의 PBS 중의 10 μM hMDM2를 첨가한다. 따라서, 각각의 8.0 ㎕ 실험 샘플은 PBS 중의 5.0 μM 농도로 40 pmol(1.5 ㎍)의 단백질, 1 μM 펩티도미메틱 거대고리 및 2.5% DMSO를 함유한다. 이로써 각각의 농도 점에 대해 제조된 중복 샘플을 실온에서 60분 동안 인큐베이션한 후, 5.0 ㎕ 주입물의 크기 배제 크로마토그래피-LC-MS 분석 전에 4℃로 냉각시킨다. 표적 단백질, 단백질-리간드 착물 및 비결합된 화합물을 함유하는 샘플을 SEC 컬럼 상에 주입하는데, 이때 상기 착물은 신속한 SEC 단계에 의해 비결합 성분으로부터 분리된다. UV 검출기를 이용하여 SEC 컬럼 용출물을 모니터링하여, SEC 컬럼의 공극 용적에서 용출되는 초기 용출 단백질 분획이 컬럼 상에 보유된 비결합된 성분으로부터 잘 분리되는 것을 확인한다. 단백질 및 단백질-리간드 착물을 함유하는 피크는 1차 UV 검출기로부터 용출된 후 샘플 루프(loop)로 들어가고, 이 루프에서 SEC 단계의 유동 스트림으로부터 제거되고 밸빙(valving) 기작을 통해 LC-MS로 직접적으로 옮겨진다. 펩티도미메틱 거대고리의 (M + 3H)3+ 이온을 예측된 m/z에서 ESI-MS로 관찰하여 단백질-리간드 착물의 검출을 확인한다.
단백질-리간드 K d 적정 실험에 대한 분석
단백질에 대한 테스트 화합물의 결합 및 친화성을 평가하기 위해, 예를 들면, 단백질-리간드 Kd 적정 실험을 수행한다. 단백질-리간드 Kd 적정 실험을 다음과 같이 수행한다: 적정제 펩티도미메틱 거대고리의 연속적으로 희석된 저장 용액(5 mM, 2.5 mM, ..., 0.098 mM)의 2 ㎕ DMSO 분취액을 제조한 후 38 ㎕의 PBS에 용해시킨다. 생성된 용액을 반복된 피펫팅으로 혼합하고 10,000 g에서 10분 동안 원심분리하여 정화한다. 생성된 상청액의 4.0 ㎕ 분취액에 4.0 ㎕의 PBS 중의 10 μM hMDM2를 첨가한다. 따라서, 각각의 8.0 ㎕ 실험 샘플은 PBS 중의 5.0 μM 농도로 40 pmol(1.5 ㎍)의 단백질, 다양한 농도(125 μM, 62.5 μM, ..., 0.24 μM)의 적정제 펩티드 및 2.5% DMSO를 함유한다. 이로써 각각의 농도 점에 대해 제조된 중복 샘플을 실온에서 30분 동안 인큐베이션한 후, 2.0 ㎕ 주입물의 SEC-LC-MS 분석 전에 4℃로 냉각시킨다. (M + H)1+, (M + 2H)2+, (M + 3H)3+ 및/또는 (M + Na)1+ 이온을 ESI-MS로 관찰하고, 추출된 이온 크로마토그램을 정량한 후, 문헌들("A General Technique to Rank Protein-Ligand Binding Affinities and Determine Allosteric vs. Direct Binding Site Competition in Compound Mixtures." Annis, D. A.; Nazef, N.; Chuang, C. C.; Scott, M. P.; Nash, H. M. J. Am. Chem. Soc. 2004, 126, 15495-15503; "ALIS: An Affinity Selection-Mass Spectrometry System for the Discovery and Characterization of Protein-Ligand Interactions" D. A. Annis, C.-C. Chuang, and N. Nazef. In Mass Spectrometry in Medicinal Chemistry. Edited by Wanner K, Hofner G: Wiley-VCH; 2007:121-184; Mannhold R, Kubinyi H, Folkers G (Series Editors): Methods and Principles in Medicinal Chemistry)에 기재된 바와 같이 방정식에 피팅하여 결합 친화성 Kd를 유도한다: .
친화성 선택-질량 분광측정에 의한 경쟁 결합 실험에 대한 분석
단백질에 경쟁적으로 결합하는 테스트 화합물의 능력을 측정하기 위해, 예를 들면, 친화성 선택-질량 분광측정 분석을 수행한다. 3개 화합물 각각의 400 μM 저장액의 2 ㎕ 분취액을 14 ㎕의 DMSO와 조합하여 성분 당 40 μM의 리간드 혼합물을 제조한다. 그 다음, 상기 성분 당 40 μM 혼합물의 1 ㎕ 분취액을 적정제 펩티도미메틱 거대고리(10 mM, 5 mM, 2.5 mM, ..., 0.078 mM)의 연속적으로 희석된 저장 용액의 1 ㎕ DMSO 분취액과 조합한다. 이들 2 ㎕의 샘플들을 38 ㎕의 PBS에 용해시킨다. 생성된 용액을 반복된 피펫팅으로 혼합하고 10,000 g에서 10분 동안 원심분리하여 정화한다. 생성된 상청액의 4.0 ㎕ 분취액에 4.0 ㎕의 PBS 중의 10 μM hMDM2 단백질을 첨가한다. 따라서, 각각의 8.0 ㎕ 실험 샘플은 PBS 중의 5.0 μM 농도로 40 pmol(1.5 ㎍)의 단백질, 0.5 μM 리간드, 2.5% DMSO 및 다양한 농도(125 μM, 62.5 μM, ..., 0.98 μM)의 적정제 펩티도미메틱 거대고리를 함유한다. 이로써 각각의 농도 점에 대해 제조된 중복 샘플을 실온에서 60분 동안 인큐베이션한 후, 2.0 ㎕ 주입물의 SEC-LC-MS 분석 전에 4℃로 냉각시킨다. 이들 방법 및 다른 방법에 대한 추가 세부설명은 문헌들("A General Technique to Rank Protein-Ligand Binding Affinities and Determine Allosteric vs. Direct Binding Site Competition in Compound Mixtures." Annis, D. A.; Nazef, N.; Chuang, C. C.; Scott, M. P.; Nash, H. M. J. Am. Chem. Soc. 2004, 126, 15495-15503; "ALIS: An Affinity Selection-Mass Spectrometry System for the Discovery and Characterization of Protein-Ligand Interactions" D. A. Annis, C.-C. Chuang, and N. Nazef. In Mass Spectrometry in Medicinal Chemistry. Edited by Wanner K, Hofner G: Wiley-VCH; 2007:121-184; Mannhold R, Kubinyi H, Folkers G (Series Editors): Methods and Principles in Medicinal Chemistry)에 제공되어 있다.
온전한 세포에서의 결합 분석
면역침전 실험으로 온전한 세포에서 펩티드 또는 펩티도미메틱 거대고리와 그들의 천연 수용체의 결합을 측정할 수 있다. 예를 들면, 온전한 세포를 혈청의 부재 하에 플루오레세인 표지된(FITC 표지된) 화합물과 함께 4시간 동안 인큐베이션한 후, 혈청을 교체하고 4시간 내지 18시간 동안 더 인큐베이션한다. 그 다음, 세포를 펠렛화하고 4℃에서 용해 완충제(50 mM 트리스[pH 7.6], 150 mM NaCl, 1% CHAPS 및 프로테아제 억제제 칵테일)에서 10분 동안 인큐베이션한다. 추출물을 14,000 rpm에서 15분 동안 원심분리하고, 상청액을 수집하고 4℃에서 회전하면서 10 ㎕의 염소 항-FITC 항체와 함께 2시간 동안 인큐베이션한 후, 4℃에서 단백질 A/G 세파로스(50 ㎕의 50% 비드 슬러리)와 함께 2시간 동안 더 인큐베이션한다. 신속한 원심분리 후, 증가하는 염 농도(예를 들면, 150 mM, 300 mM, 500 mM)를 함유하는 용해 완충제에서 펠렛을 세척한다. 그 다음, 상기 비드를 150 mM NaCl에서 재평형화시킨 후, SDS 함유 샘플 완충제를 첨가하고 끓인다. 원심분리 후, 4% 내지 12% 구배 비스-트리스 겔을 사용하여 상청액을 임의적으로 전기영동한 후 임모빌론(Immobilon)-P 막으로 옮긴다. 차단 후, 임의적으로 FITC를 검출하는 항체 및 펩티도미메틱 거대고리에 결합하는 단백질을 검출하는 하나 이상의 항체와 함께 블롯을 인큐베이션한다.
세포 침투능 분석
펩티도미메틱 거대고리는 예를 들면, 상응하는 비가교결합된 거대고리에 비해 더 높은 세포 침투능을 나타낸다. 최적화된 연결제를 갖는 펩티도미메틱 거대고리는 예를 들면, 상응하는 비가교결합된 거대고리보다 2배 이상 더 높은 세포 침투능을 보유하고, 종종 적용된 펩티도미메틱 거대고리의 20% 이상이 4시간 후 세포를 침투하는 것으로 관찰될 것이다. 펩티도미메틱 거대고리 및 상응하는 비가교결합된 거대고리의 세포 침투능을 측정하기 위해, 온전한 세포를 37℃에서 무혈청 배지에서 플루오레세인 표지된 펩티도미메틱 거대고리 또는 상응하는 비가교결합된 거대고리(10 μM)와 함께 4시간 동안 인큐베이션하고, 배지로 2회 세척하고, 37℃에서 트립신(0.25%)과 함께 10분 동안 인큐베이션한다. 상기 세포를 다시 세척하고 PBS에 재현탁한다. 예를 들면, FACS칼리부르 유동세포분석기(FACSCalibur flow cytometer) 또는 셀로믹스 키네틱스캔(Cellomics'KineticScan)® HCS 판독기를 이용하여 세포 형광도를 분석한다.
세포 효능 분석
예를 들면, 인간 또는 마우스 세포 집단으로부터 유래된 다양한 종양형성 세포주, 비종양형성 세포주 및 1차 세포를 사용하는, 세포에 기초한 사멸 분석에서 일부 펩티도미메틱 거대고리의 효능을 측정한다. 세포 생존능을 펩티도미메틱 거대고리(0.5 μM 내지 50 μM)와의 24시간 내지 96시간의 인큐베이션에 걸쳐 모니터링하여 10 μM 미만의 EC50에서 사멸하는 세포를 확인한다. 세포 생존능을 측정하는 여러 표준 분석은 상업적으로 이용가능하고 임의적으로 펩티도미메틱 거대고리의 효능을 평가하는 데에 이용된다. 또한, 아넥신(Annexin) V 및 캐스파제(caspase) 활성화를 측정하는 분석은 임의적으로 펩티도미메틱 거대고리가 아폽토시스 기구를 활성화시켜 세포를 사멸하는지를 평가하는 데에 이용된다. 예를 들면, 세포 생존능을 세포내 ATP 농도의 함수로서 측정하는 세포 타이터-글로(Titer-glo) 분석을 이용한다.
생체내 안정성 분석
펩티도미메틱 거대고리의 생체내 안정성을 조사하기 위해, 화합물을 예를 들면, 0.1 ㎎/㎏ 내지 50 ㎎/㎏의 농도로 IV, IP, PO 또는 흡입 경로를 통해 마우스 및/또는 래트에게 투여하고, 주사 후 0분, 5분, 15분, 30분, 1시간, 4시간, 8시간 및 24시간에서 혈액 표본을 채취한다. 그 다음, 25 ㎕의 새로운 혈청 중의 온전한 화합물의 수준을 전술된 바와 같이 LC-MS/MS로 측정한다.
동물 모델에서 생체내 효능
생체 내에서 본 발명의 펩티도미메틱 거대고리의 항-발암 활성을 측정하기 위해, 화합물을 예를 들면, 단독으로(IP, IV, PO, 흡입에 의해 또는 비강 경로) 또는 준최적(sub-optimal) 투여량의 관련 화학요법(예를 들면, 사이클로포스프아미드, 독소루비신, 에토포사이드)과 조합하여 제공한다. 한 예에서, NOD-SCID 마우스를 전신 방사선조사에 노출시킨 지 3시간 후, 루시퍼라제를 안정하게 발현하는 5 x 106개의 RS4;11 세포(급성 림프모구성 백혈병을 갖는 환자의 골수로부터 확립됨)를 꼬리 정맥을 통해 상기 NOD-SCID 마우스에 주입한다. 비치료된 상태로 방치되면, 이 형태의 백혈병은 이 모델에서 3주 이내에 치명적이다. 예를 들면, D-루시페린(60 ㎎/㎏)을 상기 마우스에 주입하고 마취된 동물을 영상화하여(예를 들면, 제노겐(Xenogen) 생체내 영상화 시스템, 칼리퍼 라이프 사이언시스(Caliper Life Sciences), 미국 매사추세츠주 홉킨톤 소재) 상기 백혈병을 용이하게 모니터링한다. 광자속(광자/초)을 리빙 이미지(Living Image) 소프트웨어(칼리퍼 라이프 사이언시스, 미국 매사추세츠주 홉킨톤 소재)로 적분하여 전신 생체발광도를 정량한다. 펩티도미메틱 거대고리를 단독으로 또는 준최적 투여량의 관련 화학요법 약제와 조합하여 예를 들면, 0.1 ㎎/㎏ 내지 50 ㎎/㎏의 투여량으로 꼬리 정맥 또는 IP 경로를 통해 백혈병 마우스(14 내지 16의 생체발광도 범위 내에서 주사 후 10일/실험의 1일)에게 7일 내지 21일 동안 투여한다. 임의적으로, 상기 마우스를 실험 전체에 걸쳐 격일제로 영상화하고 실험의 지속기간 동안 생존을 매일 모니터링한다. 숨진 마우스를 임의적으로 실험의 말기에 부검한다. 또 다른 동물 모델은 루시퍼라제를 안정하게 발현하는, 인간 여포성 림프종으로부터 유래된 세포주인 DoHH2의 NOD-SCID 마우스 내로의 이식이다. 이들 생체내 테스트는 임의적으로 예비 약동학, 약력학 및 독성학 데이터를 발생시킨다.
임상 시험
인간 치료를 위한 본 발명의 펩티도미메틱 거대고리의 적합성을 확인하기 위해, 임상 시험을 수행한다. 예를 들면, 암으로 진단되고 치료가 필요한 환자를 선택하고 치료군과 하나 이상의 대조군으로 분리하는데, 이때 치료군은 본 발명의 펩티도미메틱 거대고리를 투여받는 반면, 대조군은 플라세보 또는 공지된 항암 약물을 제공받는다. 따라서, 인자, 예컨대, 생존 및 삶의 질에 대한 환자 군의 비교를 수행하여 본 발명의 펩티도미메틱 거대고리의 치료 안전성 및 효능을 평가할 수 있다. 이 예에서, 펩티도미메틱 거대고리로 치료받은 환자 군은 플라세보로 치료받은 환자 대조군에 비해 개선된 장기간 생존을 보인다.
약학 조성물 및 투여 경로
본 발명의 펩티도미메틱 거대고리는 약학적으로 허용가능한 유도체 또는 이의 전구약물도 포함한다. "약학적으로 허용가능한 유도체"는 수용자에게 투여되었을 때 본 발명의 화합물을 (직접적으로 또는 간접적으로) 제공할 수 있는, 본 발명의 화합물의 임의의 약학적으로 허용가능한 염, 에스테르, 에스테르의 염, 전구약물 또는 다른 유도체를 의미한다. 특히 유리한 약학적으로 허용가능한 유도체는 포유동물에게 투여되었을 때 (예를 들면, 경구 투여된 화합물의 혈액 내로의 흡수를 증가시킴으로써) 본 발명의 화합물의 생체이용성을 증가시키거나 모 종(parent species)에 비해 상대적으로 생물학적 구획(예를 들면, 뇌 또는 림프 시스템)으로의 활성 화합물의 전달을 증가시키는 유도체이다. 몇몇 약학적으로 허용가능한 유도체는 수용성을 증가시키거나 위장 점막을 횡단하는 능동 수송을 증가시키는 화학적 기를 포함한다.
몇몇 실시양태에서, 선택적 생물학적 성질을 향상시키기 위해 적절한 작용기를 공유적으로 또는 비공유적으로 연결하여 본 발명의 펩티도미메틱 거대고리를 변형시킨다. 이러한 변형은 소정의 생물학적 구획(예를 들면, 혈액, 림프 시스템, 중추신경계) 내로의 생물학적 침투를 증가시키고, 경구 이용성을 증가시키고, 주사에 의한 투여를 허용하도록 가용성을 증가시키고, 대사를 변경하고 배출 속도를 변경하는 변형을 포함한다.
본 발명의 화합물의 약학적으로 허용가능한 염은 약학적으로 허용가능한 무기 및 유기 산 및 염기로부터 유도된 염을 포함한다. 적합한 산 염의 예로는 아세트산염, 아디프산염, 벤조산염, 벤젠설폰산염, 부티르산염, 시트르산염, 디글루콘산염, 도데실황산염, 포름산염, 푸마르산염, 글리콜산염, 반황산염, 헵탄산염, 헥산산염, 염화수소산염, 브롬화수소산염, 요오드화수소산염, 젖산염, 말레산염, 말론산염, 메탄설폰산염, 2-나프탈렌설폰산염, 니코틴산염, 질산염, 팔모산염, 인산염, 피크르산염, 피발산염, 프로피온산염, 살리실산염, 석신산염, 황산염, 주석산염, 토실산염 및 운데칸산염이 있다. 적절한 염기로부터 유도된 염은 알칼리 금속(예를 들면, 나트륨), 알칼리 토금속(예를 들면, 마그네슘), 암모늄 및 N-(알킬)4 + 염을 포함한다.
본 발명의 화합물의 약학 조성물을 제조하기 위한 약학적으로 허용가능한 담체는 고체 또는 액체 담체를 포함한다. 고체 형태 제제는 산제, 정제, 환제, 캡슐제, 카세제, 좌약제 및 분산가능한 과립제를 포함한다. 고체 담체는 희석제, 향미제, 결합제, 보존제, 정제 붕해제 또는 캡슐화 물질로도 작용하는 하나 이상의 물질일 수 있다. 제제화 및 투여를 위한 기법들에 대한 세부설명은 과학 및 특허 문헌에 잘 기재되어 있다(예를 들면, 문헌(Remington's Pharmaceutical Sciences, Maack Publishing Co, Easton PA)의 마지막 개정판 참조).
산제에서, 담체는 미분된 활성 성분을 갖는 혼합물로 존재하는 미분된 고체이다. 정제에서, 활성 성분은 필요한 결합 성질을 갖는 담체와 적합한 비율로 혼합되고 원하는 형태 및 크기로 압축된다.
적합한 고체 부형제는 락토스, 수크로스, 만니톨 또는 소르비톨을 포함하는 당; 옥수수, 밀, 쌀, 감자 또는 다른 식물로부터의 전분; 셀룰로스, 예컨대, 메틸 셀룰로스, 하이드록시프로필메틸셀룰로스 또는 나트륨 카복시메틸셀룰로스; 및 아라비아 검(gum) 및 트라가칸쓰 검을 포함하는 검; 및 단백질, 예컨대, 젤라틴 및 콜라겐을 포함하나 이들로 한정되지 않는 탄수화물 또는 단백질 충전제이다. 원하는 경우, 붕해제 또는 가용화제, 예컨대, 가교결합된 폴리비닐 피롤리돈, 아가, 알긴산 또는 이의 염, 예컨대, 알긴산나트륨이 첨가된다.
액체 형태 제제는 용액, 현탁액 및 유화액, 예를 들면, 물 또는 물/프로필렌 글리콜 용액을 포함한다. 비경구 주사의 경우, 액체 제제는 수성 폴리에틸렌 글리콜 용액 중의 용액으로 제제화될 수 있다.
약학 제제는 바람직하게는 유닛 제형으로 존재한다. 이러한 제형에서, 상기 제제는 적절한 양의 활성 성분을 함유하는 유닛 투여량으로 세분된다. 유닛 제형은 포장된 제제일 수 있고, 포장물은 분리된 양의 제제, 예컨대, 묶음 형태의 정제, 캡슐제, 및 바이알 또는 앰플 내의 산제를 함유한다. 또한, 유닛 제형은 캡슐제, 정제, 카세제 또는 로젠지 자체일 수 있거나 포장된 형태의 이들 제제 중 적절한 수의 임의의 제제일 수 있다.
본 발명의 조성물이 펩티도미메틱 거대고리와 하나 이상의 추가 치료 또는 예방 약제의 조합물을 포함하는 경우, 상기 화합물 및 추가 약제 둘 다 단일요법 섭생법에서 통상적으로 투여되는 투여량의 약 1% 내지 100%, 보다 바람직하게는 약 5% 내지 95%의 투여량 수준으로 존재해야 한다. 몇몇 실시양태에서, 상기 추가 약제는 다회 투약 섭생법의 일부로서 본 발명의 화합물과 별도로 투여된다. 대안적으로, 상기 약제는 단일 조성물에서 본 발명의 화합물과 함께 혼합된 단일 제형의 일부이다.
사용 방법
한 양태에서, 본 발명은 단백질 또는 펩티드(이에 대한 펩티도미메틱 거대고리가 모델링됨)의 천연 리간드(들)에 결합하는 약제를 확인하기 위한 경쟁 결합 분석에서 유용한 신규 펩티도미메틱 거대고리를 제공한다. 예를 들면, p53/HDMX 시스템에서, p53에 기초한 표지된 펩티도미메틱 거대고리는 HDMX에 경쟁적으로 결합하는 소분자와 함께 HDMX 결합 분석에서 사용될 수 있다. 경쟁 결합 연구는 p53/HDMX 시스템에 대해 특이적인 약물 후보물질의 신속한 시험관내 평가 및 측정을 가능하게 한다. 본원에 개시된 펩티도미메틱 거대고리 중 임의의 펩티도미메틱 거대고리 및 이의 결합 파트너를 사용하여 이러한 결합 연구를 수행할 수 있다.
본 발명은 펩티도미메틱 거대고리에 대한 항체의 발생을 추가로 제공한다. 몇몇 실시양태에서, 이들 항체는 펩티도미메틱 거대고리, 및 이 펩티도미메틱 거대고리와 관련된 전구체 펩티드, 예컨대, p53 둘 다에 특이적으로 결합한다. 이러한 항체는 예를 들면, 천연 단백질-단백질 상호작용, 예를 들면, p53과 HDMX 사이의 결합을 파괴한다.
다른 양태에서, 본 발명은 p53, HDM2 또는 HDMX를 포함하는 분자의 비정상적(예를 들면, 불충분한 또는 과도한) 발현 또는 활성과 관련된 장애의 위험(또는 민감성)을 갖거나 이러한 장애를 갖는 대상체를 치료하는 예방 방법 및 치료 방법 둘 다를 제공한다.
또 다른 실시양태에서, 장애는 적어도 부분적으로 p53, HDM2 또는 HDMX의 비정상적 수준(예를 들면, 과도한 또는 부족한 발현), 또는 비정상적 활성을 나타내는 p53, HDM2 또는 HDMX의 존재에 의해 야기된다. 따라서, p53으로부터 유도된 펩티도미메틱 거대고리에 의한 p53, HDM2 또는 HDMX의 수준 및/또는 활성의 감소, 또는 p53, HDM2 또는 HDMX의 수준 및/또는 활성의 향상이 예를 들면, 상기 장애의 불리한 증상을 호전시키거나 감소시키는 데에 이용된다.
또 다른 양태에서, 본 발명은 결합 파트너들 사이, 예를 들면, p53과 HDM2 사이 또는 p53과 HDMX 사이의 상호작용 또는 결합을 방해함으로써 과증식성 질환 및 염증성 장애를 포함하는 질환을 치료하거나 예방하는 방법을 제공한다. 이들 방법은 유효량의 본 발명의 화합물을 인간을 포함하는 온혈동물에게 투여하는 단계를 포함한다. 몇몇 실시양태에서, 본 발명의 화합물의 투여는 세포 성장 정지 또는 아폽토시스를 유도한다.
본원에서 사용된 바와 같이, 용어 "치료"는 질환, 질환의 증상 또는 질환에 대한 소인을 치료하거나, 치유하거나, 완화하거나, 경감시키거나, 변경하거나, 교정하거나, 호전시키거나, 개선하거나, 또는 영향을 미치기 위한 목적으로 치료제를, 질환, 질환의 증상 또는 질환에 대한 소인을 갖는 환자에게 적용하거나 투여하거나, 또는 치료제를 상기 환자로부터 단리된 조직 또는 세포주에 적용하거나 투여하는 것으로서 정의된다.
몇몇 실시양태에서, 본 발명의 펩티도미메틱 거대고리는 암 및 신생물성 병태의 치료, 예방 및/또는 진단을 위해 사용된다. 본원에서 사용된 바와 같이, 용어 "암", "과증식성" 및 "신생물성"은 자율적 성장 능력을 갖는 세포, 즉 신속하게 증식하는 세포 성장을 특징으로 하는 비정상적 상태 또는 병태를 지칭한다. 과증식성 및 신생물성 질환 상태는 병리학적 상태로서, 즉 질환 상태를 특징짓거나 구성하는 상태로서 분류될 수 있거나, 또는 비병리학적 상태로서, 즉 정상 상태로부터 벗어나 있지만 질환 상태와 관련되지 않은 상태로서 분류될 수 있다. 상기 용어는 침윤의 조직학적 유형 또는 단계와 관계없이 모든 유형의 암성 성장 또는 발암성 과정, 전이성 조직 또는 악성으로 형질전환된 세포, 조직 또는 기관을 포함하기 위한 것이다. 전이성 종양은 유방, 폐, 간, 결장 및 난소 유래의 원발성 종양 유형을 포함하나 이들로 한정되지 않는 다수의 원발성 종양 유형으로부터 발생할 수 있다. "병리학적 과증식성" 세포는 악성 종양 성장을 특징으로 하는 질환 상태에서 발생한다. 비병리학적 과증식성 세포의 예로는 상처 회복과 관련된 세포의 증식을 포함한다. 세포 증식성 및/또는 분화성 장애의 예로는 암, 예를 들면, 암종, 육종 또는 전이성 장애가 있다. 몇몇 실시양태에서, 펩티도미메틱 거대고리는 유방암, 난소암, 결장암, 폐암, 이러한 암의 전이 등을 제어하기 위한 신규 치료제가다.
암 또는 신생물성 병태의 예로는 섬유육종, 근육종, 지방육종, 연골육종, 골원성 육종, 척삭종, 혈관육종, 내피육종, 림프관육종, 림프관내피육종, 활막종, 중피종, 에윙 종양, 평활근육종, 횡문근육종, 위암, 식도암, 직장암, 췌장암, 난소암, 전립선암, 자궁암, 두경부암, 피부암, 뇌암, 편평세포 암종, 피지선 암종, 유두 암종, 유두 선암종, 낭선암종, 수질 암종, 기관지원성 암종, 신장 세포 암종, 간세포종, 담관 암종, 융모막암종, 고환종, 배아 암종, 윌름 종양, 자궁경부암, 정소암, 소세포 폐 암종, 비소세포 폐 암종, 방광 암종, 상피 암종, 아교모세포종, 성상세포종, 수모세포종, 두개인두종, 뇌실막종, 송과체종, 혈관모세포종, 청신경초종, 희소돌기아교세포종, 수막종, 흑색종, 신경모세포종, 망막모세포종, 백혈병, 림프종 또는 카포시 육종이 있으나 이들로 한정되지 않는다.
증식성 장애의 예로는 조혈 신생물성 장애가 있다. 본원에서 사용된 바와 같이, 용어 "조혈 신생물성 장애"는 조혈 유래의, 예를 들면, 골수, 림프구 또는 적혈구 계통, 또는 이들의 전구체 세포로부터 발생된 과형성/신생물성 세포를 수반하는 질환을 포함한다. 바람직하게는, 상기 질환은 저분화된(poorly differentiated) 급성 백혈병, 예를 들면, 적혈모구성 백혈병 및 급성 거대모구성 백혈병으로부터 발생한다. 추가 예시적 골수 장애는 급성 전골수성(promyeloid) 백혈병(APML), 급성 골수성 백혈병(AML) 및 만성 골수성 백혈병(CML)(문헌(Vaickus (1991), Crit Rev. Oncol./Hemotol. 11:267-97)에서 검토됨)을 포함하나 이들로 한정되지 않고, 림프 악성종양은 B 계통 급성 림프모구성 백혈병(ALL) 및 T 계통 ALL을 포함하는 ALL, 만성 림프구성 백혈병(CLL), 전림프구성 백혈병(PLL), 모발세포 백혈병(HLL) 및 발덴스트롬 마크로글로불린혈증(WM)을 포함하나 이들로 한정되지 않는다. 악성 림프종의 추가 형태는 비호지킨 림프종 및 이의 변이체, 말초 T 세포 림프종, 성인 T 세포 백혈병/림프종(ATL), 피부 T 세포 림프종(CTCL), 대과립 림프구성 백혈병(LGF), 호지킨병 및 리드-스템베르그(Reed-Stemberg)병을 포함하나 이들로 한정되지 않는다.
유방의 세포 증식성 및/또는 분화성 장애의 예로는 예를 들면, 상피 과형성증, 경화성 선증 및 소관 유두종을 포함하는 증식성 유방 질환; 종양, 예를 들면, 간질 종양, 예컨대, 섬유선종, 엽상 종양 및 육종, 및 상피 종양, 예컨대, 대관 유두종; 유관 상피내 암종(파제트병을 포함함) 및 소엽성 상피내 암종을 포함하는 상피내(비침습성) 암종, 및 침습성 유관 암종, 침습성 소엽성 암종, 수질 암종, 콜로이드(점액) 암종, 관 암종 및 침습성 유두 암종을 포함하나 이들로 한정되지 않는 침습성(침윤성) 암종을 포함하는 유방 암종; 및 잡종 악성 신생물이 있으나 이들로 한정되지 않는다. 남성 유방에서의 장애는 여성유방증 및 암종을 포함하나 이들로 한정되지 않는다.
폐의 세포 증식성 및/또는 분화성 장애의 예로는 부신생물성(paraneoplastic) 증후군, 세기관지폐포 암종, 신경내분비 종양, 예컨대, 기관지 유암종, 잡종 종양 및 전이성 종양을 포함하는 기관지원성 암종; 및 염증성 흉막 삼출, 비염증성 흉막 삼출, 기흉, 및 흉막 종양(고립 섬유 종양(흉막 섬유종) 및 악성 중피종을 포함함)을 포함하는 흉막의 병리학적 상태가 있으나 이들로 한정되지 않는다.
결장의 세포 증식성 및/또는 분화성 장애의 예로는 비신생물성 폴립, 선종, 가족성 증후군, 대장 발암, 대장 암종 및 카시노이드(carcinoid) 종양이 있으나 이들로 한정되지 않는다.
간의 세포 증식성 및/또는 분화성 장애의 예로는 결절 과형성, 선종 및 악성 종양(간의 원발성 암종 및 전이성 종양을 포함함)이 있으나 이들로 한정되지 않는다.
난소의 세포 증식성 및/또는 분화성 장애의 예로는 난소 종양, 예컨대, 체강 상피 종양, 장액 종양, 점액 종양, 자궁내막유사 종양, 투명 세포 선암종, 낭선섬유종, 브렌너(Brenner) 종양, 표면 상피 종양; 생식세포 종양, 예컨대, 성숙 (양성) 기형종, 단일진피성 기형종, 미성숙 악성 기형종, 미분화세포종, 내배엽동 종양, 융모막암종; 성삭 기질 종양, 예컨대, 과립난포막 세포 종양, 난포막종섬유종, 남성모세포종, 힐(hill) 세포 종양 및 성선모세포종; 및 전이성 종양, 예컨대, 크루켄베르그(Krukenberg) 종양이 있으나 이들로 한정되지 않는다.
다른 또는 추가 실시양태에서, 본원에 기재된 펩티도미메틱 거대고리는 생리학적 손상 등으로 인한 과활성 세포 사멸 또는 세포성 사멸을 특징으로 하는 병태의 치료, 예방 또는 진단을 위해 사용된다. 미성숙 또는 원치 않는 세포 사멸, 또는 대안적으로 원치 않는 또는 과도한 세포 증식을 특징으로 하는 병태들의 몇몇 예로는 저세포성/저형성, 무세포성/무형성 또는 과세포성/과형성 병태가 있으나 이들로 한정되지 않는다. 몇몇 예로는 판코니(fanconi) 빈혈, 무형성 빈혈, 지중해빈혈, 선천성 호중구감소증 및 골수이형성증을 포함하나 이들로 한정되지 않는 혈액 장애가 있다.
다른 또는 추가 실시양태에서, 아폽토시스를 감소시키는 작용을 하는 본 발명의 펩티도미메틱 거대고리는 바람직하지 않은 수준의 세포 사멸과 관련된 장애의 치료를 위해 사용된다. 따라서, 몇몇 실시양태에서, 본 발명의 항-아폽토시스성 펩티도미메틱 거대고리는 장애, 예컨대, 바이러스 감염, 예를 들면, 인간 면역결핍 바이러스(HIV)에 의한 감염과 관련된 감염과 관련된 세포 사멸을 초래하는 장애의 치료를 위해 사용된다. 매우 다양한 신경 질환들이 특정 신경 세트(set)의 점차적인 손실을 특징으로 한다. 한 예는 알쯔하이머병(AD)이다. 알쯔하이머병은 대뇌 피질 및 일부 피질하 영역 내의 신경 및 시냅시스의 손실을 특징으로 한다. 이 손실은 영향받은 영역의 심한 위축을 발생시킨다. 아밀로이드 플라크 및 신경원섬유매듭이 AD에 의해 고통받는 환자들의 뇌에서 가시화된다. 알쯔하이머병은 뇌 내에서의 비정상적으로 폴딩된 A-베타 및 타우(tau) 단백질의 축적으로 인한 단백질 미스폴딩(misfolding) 질환으로서 확인되었다. 플라크는 β-아밀로이드로 구성된다. β-아밀로이드는 아밀로이드 전구체 단백질(APP)로 지칭되는 보다 큰 단백질의 단편이다. APP는 신경 성장, 생존 및 손상 후 회복에 있어서 매우 중요하다. AD에서, 미공지된 과정은 APP가 단백질용해를 통해 효소에 의해 더 작은 단편으로 절단되게 한다. 이들 단편 중 하나는 노인성 플라크로서 공지된 조밀한 형성물 형태로 신경 외부에서 침착되는 덩어리를 형성하는 β-아밀로이드의 원섬유(fibril)이다. 플라크는 신경 세포 내에서 종종 매듭으로 지칭되는 불용성 비틀린 섬유로 계속 성장한다. 따라서, β-아밀로이드와 그의 천연 수용체 사이의 상호작용의 파괴는 AD의 치료에 있어서 중요하다. 몇몇 실시양태에서, 본 발명의 항-아폽토시스성 펩티도미메틱 거대고리는 세포 아폽토시스와 관련된 AD 및 다른 신경 장애의 치료에 사용된다. 이러한 신경 장애는 알쯔하이머병, 파킨슨병, 근위축측삭경화증(ALS), 색소망막염, 척수근위축증 및 다양한 형태의 소뇌 변성을 포함한다. 이들 질환들에서 세포 손실은 염증성 반응을 유도하지 않고, 아폽토시스는 세포 사멸의 기작인 것으로 보인다.
또한, 다수의 혈액 질환이 혈액 세포의 감소된 생성과 관련된다. 이들 장애는 만성 질환과 관련된 빈혈, 무형성 빈혈, 만성 호중구감소증 및 골수형성이상 증후군을 포함한다. 혈액 세포 생성의 장애, 예컨대, 골수형성이상 증후군 및 몇몇 형태의 무형성 빈혈은 골수 내에서의 증가된 아폽토시스성 세포 사멸과 관련된다. 이들 장애는 아폽토시스를 촉진하는 유전자의 활성화, 간질 세포 또는 조혈 생존 인자에서의 후천적 결핍, 또는 독소 및 면역 반응의 매개자의 직접적 효과로부터 발생할 수 있다. 세포 사멸과 관련된 2종의 공통된 장애는 심근경색 및 뇌졸중이다. 두 장애에서, 혈류의 급성 손실이 일어나는 경우 발생하는 허혈의 중심 영역 내의 세포가 괴사의 결과로서 신속히 사멸하는 것으로 보인다. 그러나, 중심 허혈 영역 외부에서 세포는 보다 연장된 시간에 걸쳐 사멸하고 형태학적으로 아폽토시스에 의해 사멸하는 것으로 보인다. 다른 또는 추가 실시양태에서, 본 발명의 항-아폽토시스성 펩티도미메틱 거대고리는 바람직하지 않은 세포 사멸과 관련된 모든 이러한 장애들의 치료를 위해 사용된다.
본원에 기재된 펩티도미메틱 거대고리에 의해 치료되는 신경 장애의 몇몇 예로는 알쯔하이머병, 다운증후군, 더치(Dutch) 유형 유전성 뇌출혈 아밀로이드증, 반응성 아밀로이드증, 두드러기 및 난청을 동반하는 가족성 아밀로이드 신장병증, 머클-웰스(Muckle-Wells) 증후군, 특발성 골수종, 마크로글로불린혈증 관련 골수종, 가족성 아밀로이드 다발신경병증, 가족성 아밀로이드 심근병증, 고립성 심장 아밀로이드, 전신 노인성 아밀로이드증, 성인 발병형 당뇨병, 인슐린종, 고립성 심방 아밀로이드, 갑상선의 수질 암종, 가족성 아밀로이드증, 아밀로이드증을 동반하는 유전성 뇌출혈, 가족성 아밀로이드 다발신경병증, 스크래피(Scrapie), 크로이츠펠트-야콥(Creutzfeldt-Jacob)병, 게르스트만 스트라우슬러-쉐인커(Gerstmann Straussler-Scheinker) 증후군, 소해면상뇌병증, 프리온 매개 질환 및 헌팅톤병이 있으나 이들로 한정되지 않는다.
또 다른 실시양태에서, 본원에 기재된 펩티도미메틱 거대고리는 염증성 장애의 치료, 예방 또는 진단을 위해 사용된다. 다수의 유형의 염증성 장애가 존재한다. 일부 염증성 질환, 예를 들면, 자가면역 질환은 면역 시스템과 관련된다. 자가면역 질환은 체내에 정상적으로 존재하는 물질 및 조직, 즉, 자가 항원에 대한 신체의 과활성 면역 반응으로부터 발생한다. 다시 말해, 면역 시스템이 그 자신의 세포를 공격한다. 자가면역 질환은 면역 매개 질환의 주요 원인이다. 류마티스성 관절염은 면역 시스템이 관절을 공격하고 관절에서 염증(즉, 관절염) 및 파괴를 야기하는 자가면역 질환의 한 예이다. 이것은 몇몇 기관, 예컨대, 폐 및 피부도 손상시킬 수 있다. 류마티스성 관절염은 기능성 및 운동성의 실질적인 손실을 초래할 수 있다. 류마티스성 관절염은 혈액 테스트, 특히 류마티스성 인자 테스트에 의해 진단된다. 본원에 기재된 펩티도미메틱 거대고리에 의해 치료되는 자가면역 질환의 몇몇 예로는 급성 파종뇌척수염(ADEM), 애디슨병, 강직척추염, 항-인지질 항체 증후군(APS), 자가면역 용혈 빈혈, 자가면역 간염, 자가면역 내이 질환, 베체트병, 수포성 유천포창, 복강 질환, 샤가스병, 처그-스트라우스(Churg-Strauss) 증후군, 만성 폐쇄성 폐 질환(COPD), 크론병, 피부근염, 1형 진성 당뇨병, 자궁내막증, 굿파스처 증후군, 그레이브병, 귈랑-바레 증후군(GBS), 하시모토병, 화농땀샘염, 특발성 혈소판감소성자반병, 염증성 장 질환(IBD), 간질성 방광염, 홍반성 루프스, 국소피부경화증, 다발성 경화증, 중증근육무력증, 기면증, 신경근육긴장증, 심상성 천포창, 악성 빈혈, 다발근육염, 류마티스성 다발근육통, 원발성 담관간경화증, 건선, 류마티스성 관절염, 정신분열증, 공피증, 쇼그렌 증후군, 측두 동맥염("거대 세포 동맥염"으로도 공지되어 있음), 타카야수 동맥염, 혈관염, 백반증 및 베게너 육아종증이 있으나 이들로 한정되지 않는다.
본원에 기재된 펩티도미메틱 거대고리에 의해 치료되는 다른 유형의 염증성 장애의 몇몇 예로는 알레르기성 비염/동염, 피부 알레르기(두드러기/발진, 혈관부종, 아토피성 피부염), 식품 알레르기, 약물 알레르기, 곤충 알레르기 및 희귀 알레르기성 장애, 예컨대, 비만세포증, 천식, 관절염(골관절염, 류마티스성 관절염 및 척추관절병증을 포함함), CNS의 원발성 혈관염, 사르코이드증, 기관 이식 거부, 섬유근육통, 섬유증, 췌장염 및 골반 염증성 질환이 있으나 이들로 한정되지 않는다.
본 발명의 펩티도미메틱 거대고리에 의해 치료되거나 예방되는 심혈관 장애(예를 들면, 염증성 장애)의 예로는 대동맥판막협착증, 죽상동맥경화증, 심근경색, 뇌졸중, 혈전증, 동맥류, 심부전, 허혈 심장 질환, 협심증, 돌연 심장사, 고혈압 심장 질환, 비관상동맥 혈관 질환, 예컨대, 세동맥경화증, 소혈관 질환, 신장병증, 고트리글리세라이드혈증, 고콜레스테롤혈증, 고지질혈증, 황색종증, 천식, 고혈압, 폐기종 및 만성 폐 질환; 또는 중재적 시술과 관련된 심혈관 병태("시술적 혈관 외상"), 예컨대, 혈관성형술, 또는 단락(shunt), 스텐트(stent), 합성 또는 천연 절제 이식물, 유치 카테터, 밸브 또는 다른 이식가능한 장치의 배치 후 재협착증이 있으나 이들로 한정되지 않는다. 바람직한 심혈관 장애는 죽상동맥경화증, 심근경색, 동맥류 및 뇌졸중을 포함한다.
본 발명의 바람직한 실시양태가 본원에 제시되고 기재되어 있지만, 이러한 실시양태가 단지 예로서 제공된 것임이 당업자에게 자명할 것이다. 본 발명을 벗어나지 않으면서 다수의 변경, 변화 및 치환이 당업자에게 인식될 것이다. 본원에 기재된 본 발명의 실시양태들에 대한 다양한 대안이 본 발명을 실시함에 있어서 사용될 수 있다는 것을 이해해야 한다. 하기 특허청구범위는 본 발명의 범위를 정의하고 이 특허청구범위 내의 방법 및 구조, 및 이들의 등가물은 하기 특허청구범위에 포함된다.
실시예
실시예 1: 6-클로로트립토판 Fmoc 아미노산의 합성
Figure pat00032
tert-부틸 6-클로로-3-포밀-1H-인돌-1-카복실레이트(화합물 1)
아르곤 하에 0℃에서 POCl3(3.92 ㎖, 43 mmol, 1.3 당량)을 무수 DMF(12 ㎖) 중의 교반된 용액에 적가하였다. 용액을 동일한 온도에서 20분 동안 교반한 후, 무수 DMF(30 ㎖) 중의 6-클로로인돌(5.0 g, 33 mmol, 1 당량)의 용액을 적가하였다. 생성된 혼합물을 실온까지 가온하고 추가 2.5시간 동안 교반하였다. 물(50 ㎖)을 첨가하였고, 용액을 4 M 수성 NaOH(pH 약 8)로 중화하였다. 생성된 고체를 여과하고 물로 세척하고 진공 하에 건조하였다. 이 물질을 추가 정제 없이 다음 단계에서 직접적으로 사용하였다. N2 하에 실온에서 Boc2O(7.91 g, 36.3 mmol, 1.1 당량) 및 DMAP(0.4 g, 3.3 mmol, 0.1 당량)를 THF(150 ㎖) 중의 미정제 포밀 인돌(33 mmol, 1 당량)의 교반된 용액에 연속적으로 첨가하였다. 생성된 혼합물을 실온에서 1.5시간 동안 교반하였고, 용매를 감압 하에 증발시켰다. 잔사를 EtOAc에 용해시키고 1 N HCl로 세척하고 농축하여 백색 고체로서 포밀 인돌(화합물 1)(9 g, 2개 단계에 걸쳐 98%)을 수득하였다. 1H NMR(CDCl3) δ: 1.70 (s, Boc, 9H); 7.35 (dd, 1H); 8.21 (m, 3H); 10.07 (s, 1H).
tert-부틸 6-클로로-3-(하이드록시메틸)-1H-인돌-1-카복실레이트(화합물 2)
NaBH4(2.4 g, 63 mmol, 2 당량)를 에탄올(150 ㎖) 중의 화합물 1(8.86 g, 32 mmol, 1 당량)의 용액에 첨가하였다. 반응물을 실온에서 3시간 동안 교반하였다. 반응 혼합물을 농축하였고, 잔사를 디에틸 에테르 및 물에 부었다. 유기층을 분리하고 황산마그네슘 상에서 건조하고 농축하여 백색 고체(8.7 g, 98%)를 수득하였다. 이 물질을 추가 정제 없이 다음 단계에서 직접적으로 사용하였다. 1H NMR(CDCl3) δ: 1.65 (s, Boc, 9H); 4.80 (s, 2H, CH2); 7.21 (dd, 1H); 7.53 (m, 2H); 8.16 (bs, 1H).
tert-부틸 3-(브로모메틸)-6-클로로-1H-인돌-1-카복실레이트(화합물 3)
아르곤 하에 -40℃에서 디클로로메탄(50 ㎖) 중의 트리페닐포스핀(4.59 g, 17.5 mmol, 1.2 당량)의 용액을 디클로로메탄(50 ㎖) 중의 화합물 2(4.1 g, 14.6 mmol, 1 당량)의 용액에 첨가하였다. 반응 용액을 40℃에서 추가 30분 동안 교반하였다. 그 다음, NBS(3.38 g, 19 mmol, 1.3 당량)를 첨가하였다. 생성된 혼합물을 실온까지 가온하고 밤새 교반하였다. 디클로로메탄을 증발시켰고, 사염화탄소(100 ㎖)를 첨가하였고, 혼합물을 1시간 동안 교반하고 여과하였다. 여과액을 농축하고 실리카 플러그에 적재하고 헥산 중의 25% EtOAc로 신속히 용출하였다. 용액을 농축하여 백색 포말(3.84 g, 77%)을 수득하였다. 1H NMR(CDCl3) δ: 1.66 (s, Boc, 9H); 4.63 (s, 2H, CH2); 7.28 (dd, 1H); 7.57 (d, 1H); 7.64 (bs, 1H); 8.18 (bs, 1H).
αMe-6Cl-Trp(Boc)-Ni-S-BPB(화합물 4)
아르곤 하에 50 ㎖의 DMF를 S-Ala-Ni-S-BPB(2.66 g, 5.2 mmol, 1 당량) 및 KO-tBu(0.87 g, 7.8 mmol, 1.5 당량)에 첨가하였다. DMF 용액(5.0 ㎖) 중의 브로마이드 유도체 화합물 3(2.68 g, 7.8 mmol, 1.5 당량)을 주사기를 통해 첨가하였다. 반응 혼합물을 주위 온도에서 1시간 동안 교반하였다. 그 다음, 용액을 5% 수성 아세트산으로 켄칭하고 물로 희석하였다. 원하는 생성물을 디클로로메탄으로 추출하고 건조하고 농축하였다. 유성 생성물(화합물 4)을, EtOAc 및 헥산을 용출제로서 사용하는 순상 상의 플래쉬 크로마토그래피(고체 적재)로 정제하여 적색 고체(1.78 g, 45% 수율)를 수득하였다. αMe-6Cl-Trp(Boc)-Ni-S-BPB, 4: M+H 계산치 775.21, M+H 실측치 775.26; 1H NMR(CDCl3) δ: 1.23 (s, 3H, αMe); 1.56 (m, 11H, Boc + CH2); 1.82-2.20 (m, 4H, 2CH2); 3.03 (m, 1H, CHα); 3.24 (m, 2H, CH2); 3.57 및 4.29 (AB 시스템, 2H, CH2(벤질), J= 12.8Hz); 6.62 (d, 2H); 6.98 (d, 1H); 7.14 (m, 2H); 7.23 (m, 1H); 7.32-7.36 (m, 5H); 7.50 (m, 2H); 7.67 (bs, 1H); 7.98 (d, 2H); 8.27 (m, 2H).
Fmoc-αMe-6Cl-Trp(Boc)-OH(화합물 6)
50℃에서 MeOH(5 ㎖) 중의 화합물 4(1.75 g, 2.3 mmol, 1 당량)의 용액을 3 N HCl/MeOH(1/3, 15 ㎖)의 용액에 적가하였다. 출발 물질은 3시간 내지 4시간 이내에 사라졌다. 그 다음, 산성 용액을 빙욕조로 0℃까지 냉각시키고 Na2CO3(1.21 g, 11.5 mmol, 5 당량)의 수용액으로 켄칭하였다. 메탄올을 제거하였고, 8 당량 이상의 Na2CO3(1.95 g, 18.4 mmol)을 현탁액에 첨가하였다. 그 다음, 니켈 소거(scavenging) EDTA 이나트륨 염 이수화물(1.68 g, 4.5 mmol, 2 당량)을 첨가하였고, 현탁액을 2시간 동안 교반하였다. 아세톤(50 ㎖) 중의 Fmoc-OSu(0.84 g, 2.5 mmol, 1.1 당량)의 용액을 첨가하였고, 반응물을 밤새 교반하였다. 그 후, 반응물을 디에틸 에테르 및 1 N HCl로 희석하였다. 그 다음, 유기층을 황산마그네슘 상에서 건조하고 진공 중에서 농축하였다. 원하는 생성물(화합물 6)을, 아세톤 및 디클로로메탄을 용출제로서 사용하는 순상 상에서 정제하여 백색 포말(0.9 g, 70% 수율)을 수득하였다. Fmoc-αMe-6Cl-Trp(Boc)-OH, 6: M+H 계산치 575.19, M+H 실측치 575.37; 1H NMR(CDCl3) 1.59 (s, 9H, Boc); 1.68 (s, 3H, Me); 3.48 (bs, 2H, CH2); 4.22 (m, 1H, CH); 4.39 (bs, 2H, CH2); 5.47 (s, 1H, NH); 7.10 (m, 1H); 7.18 (m, 2H); 7.27 (m, 2H); 7.39 (m, 2H); 7.50 (m, 2H); 7.75 (d, 2H); 8.12 (bs, 1H).
6Cl-Trp(Boc)-Ni-S-BPB(화합물 5)
아르곤 하에 95 ㎖의 DMF를 Gly-Ni-S-BPB(4.6 g, 9.2 mmol, 1 당량) 및 KO-tBu(1.14 g, 10.1 mmol, 1.1 당량)에 첨가하였다. DMF 용액(10 ㎖) 중의 브로마이드 유도체 화합물 3(3.5 g, 4.6 mmol, 1.1 당량)을 주사기를 통해 첨가하였다. 반응 혼합물을 주위 온도에서 1시간 동안 교반하였다. 그 다음, 용액을 5% 수성 아세트산으로 켄칭하고 물로 희석하였다. 원하는 생성물을 디클로로메탄으로 추출하고 건조하고 농축하였다. 유성 생성물(화합물 5)을, EtOAc 및 헥산을 용출제로서 사용하는 순상 상의 플래쉬 크로마토그래피(고체 적재)로 정제하여 적색 고체(5 g, 71% 수율)를 수득하였다. 6Cl-Trp(Boc)-Ni-S-BPB, 5: M+H 계산치 761.20, M+H 실측치 761.34; 1H NMR(CDCl3) δ: 1.58 (m, 11H, Boc + CH2); 1.84 (m, 1H); 1.96 (m, 1H); 2.24 (m, 2H, CH2); 3.00 (m, 1H, CHα); 3.22 (m, 2H, CH2); 3.45 및 4.25 (AB 시스템, 2H, CH2(벤질), J= 12.8Hz); 4.27 (m, 1H, CHα); 6.65 (d, 2H); 6.88 (d, 1H); 7.07 (m, 2H); 7.14 (m, 2H); 7.28 (m, 3H); 7.35-7.39 (m, 2H); 7.52 (m, 2H); 7.96 (d, 2H); 8.28 (m, 2H).
Fmoc-6Cl-Trp(Boc)-OH(화합물 7)
50℃에서 MeOH (10 ㎖) 중의 화합물 5(5 g, 6.6 mmol, 1 당량)의 용액을 3 N HCl/MeOH(1/3, 44 ㎖)의 용액에 적가하였다. 출발 물질은 3시간 내지 4시간 이내에 사라졌다. 그 다음, 산성 용액을 빙욕조로 0℃까지 냉각시키고 Na2CO3(3.48 g, 33 mmol, 5 당량)의 수용액으로 켄칭하였다. 메탄올을 제거하였고, 8 당량 이상의 Na2CO3(5.57 g, 52 mmol)을 현탁액에 첨가하였다. 그 다음, 니켈 소거 EDTA 이나트륨 염 이수화물(4.89 g, 13.1 mmol, 2 당량)을 첨가하였고, 현탁액을 2시간 동안 교반하였다. 아세톤(100 ㎖) 중의 Fmoc-OSu(2.21 g, 6.55 mmol, 1.1 당량)의 용액을 첨가하였고, 반응물을 밤새 교반하였다. 그 후, 반응물을 디에틸 에테르 및 1 N HCl로 희석하였다. 그 다음, 유기층을 황산마그네슘 상에서 건조하고 진공 중에서 농축하였다. 원하는 생성물(화합물 7)을, 아세톤 및 디클로로메탄을 용출제로서 사용하는 순상 상에서 정제하여 백색 포말(2.6 g, 69% 수율)을 수득하였다. Fmoc-6Cl-Trp(Boc)-OH, 7: M+H 계산치 561.17, M+H 실측치 561.37; 1H NMR(CDCl3) 1.63 (s, 9H, Boc); 3.26 (m, 2H, CH2); 4.19 (m, 1H, CH); 4.39 (m, 2H, CH2); 4.76 (m, 1H); 5.35 (d, 1H, NH); 7.18 (m, 2H); 7.28 (m, 2H); 7.39 (m, 3H); 7.50 (m, 2H); 7.75 (d, 2H); 8.14 (bs, 1H).
실시예 2: 본 발명의 펩티도미메틱 거대고리
펩티도미메틱 거대고리를 전술된 바와 같이 및 이하에 기재된 바와 같이 합성하고 정제하고 분석하였다(Schafmeister et al., J. Am. Chem. Soc. 122:5891-5892 (2000); Schafmeister & Verdine, J. Am. Chem. Soc. 122:5891 (2005); Walensky et al., Science 305:1466-1470 (2004); 및 미국 특허 제7,192,713호). 2개 이상의 천연 발생 아미노산을 상응하는 합성 아미노산으로 치환시켜 펩티도미메틱 거대고리를 디자인하였다. i 및 i+4, 및 i 및 i+7 위치에서 치환시켰다. 고체상 조건, 링크(rink) 아미드 AM 수지(노바바이오켐(Novabiochem)) 및 Fmoc 주쇄 보호기 화학반응을 이용하여 펩티드 합성을 수동으로 또는 자동화된 펩티드 합성기(어플라이드 바이오시스템스((Applied Biosystems), 모델 433A) 상에서 수행하였다. 천연 Fmoc 보호된 아미노산(노바바이오켐)의 커플링을 위해, 10 당량의 아미노산 및 1:1:2 몰비의 커플링 시약 HBTU/HOBt(노바바이오켐)/DIEA를 사용하였다. 비천연 아미노산(4 당량)을 1:1:2 몰비의 HATU(어플라이드 바이오시스템스)/HOBt/DIEA와 커플링시켰다. 합성 펩티드의 N 말단을 아세틸화한 반면, C 말단을 아미드화하였다.
가교결합된 화합물의 정제를 역상 C18 컬럼(바리안(Varian)) 상의 고성능 액체 크로마토그래피(HPLC)(바리안 프로스타(Varian ProStar))로 달성하여 순수한 화합물을 수득하였다. 순수한 생성물의 화학적 조성을 LC/MS 질량 분광측정(아질런트(Agilent) 1100 HPLC 시스템과 접속된 마이크로매스 LCT) 및 아미노산 분석(어플라이드 바이오시스템스, 모델 420A)으로 확인하였다.
하기 표 4는 제조된 본 발명의 펩티도미메틱 거대고리의 목록을 보여준다.
[표 4]
Figure pat00033
Figure pat00034
Figure pat00035
Figure pat00036
Figure pat00037
Figure pat00038
Figure pat00039
Figure pat00040
Figure pat00041
Figure pat00042
Figure pat00043
Figure pat00044
Figure pat00045
Figure pat00046
Figure pat00047
Figure pat00048
상기 및 임의의 부분에서 제시된 서열에서, 하기 약어가 사용된다: "Nle"는 노르류신을 나타내고, "Aib"는 2-아미노이소부티르산을 나타내고, "Ac"는 아세틸을 나타내고, "Pr"은 프로피오닐을 나타낸다. "$"로서 나타낸 아미노산은 1개의 이중 결합을 포함하는 모든-탄소 i 내지 i+4 가교제에 의해 연결된 α-Me S5-펜테닐-알라닌 올레핀 아미노산이다. "$r5"로서 나타낸 아미노산은 1개의 이중 결합을 포함하는 모든-탄소 i 내지 i+4 가교제에 의해 연결된 α-Me R5-펜테닐-알라닌 올레핀 아미노산이다. "$s8"로서 나타낸 아미노산은 1개의 이중 결합을 포함하는 모든-탄소 i 내지 i+7 가교제에 의해 연결된 α-Me S8-옥테닐-알라닌 올레핀 아미노산이다. "$r8"로서 나타낸 아미노산은 1개의 이중 결합을 포함하는 모든-탄소 i 내지 i+7 가교제에 의해 연결된 α-Me R8-옥테닐-알라닌 올레핀 아미노산이다. "Ahx"는 아미노사이클로헥실 연결제를 나타낸다. 가교제는 각각의 아미노산의 α 탄소들 사이에서 8개 또는 11개의 탄소 원자를 포함하는 선형 모든-탄소 가교제가다. "$/"로서 나타낸 아미노산은 임의의 가교제에 의해 연결되지 않은 α-Me S5-펜테닐-알라닌 올레핀 아미노산이다. "$/r5"로서 나타낸 아미노산은 임의의 가교제에 의해 연결되지 않은 α-Me R5-펜테닐-알라닌 올레핀 아미노산이다. "$/s8"로서 나타낸 아미노산은 임의의 가교제에 의해 연결되지 않은 α-Me S8-옥테닐-알라닌 올레핀 아미노산이다. "$/r8"로서 나타낸 아미노산은 임의의 가교제에 의해 연결되지 않은 α-Me R8-옥테닐-알라닌 올레핀 아미노산이다. "Amw"로서 나타낸 아미노산은 α-Me 트립토판 아미노산이다. "Aml"로서 나타낸 아미노산은 α-Me 류신 아미노산이다. "2ff"로서 나타낸 아미노산은 2-플루오로-페닐알라닌 아미노산이다. "3ff"로서 나타낸 아미노산은 3-플루오로-페닐알라닌 아미노산이다. "St"로서 나타낸 아미노산은 표시된 바와 같이 또 다른 아미노산에 각각 가교결합된 2개의 펜테닐-알라닌 올레핀 측쇄를 포함하는 아미노산이다. "St//"로서 나타낸 아미노산은 가교결합되지 않은 2개의 펜테닐-알라닌 올레핀 측쇄를 포함하는 아미노산이다. "%St"로서 나타낸 아미노산은 표시된 바와 같이 전체적으로 포화된 탄화수소 가교결합을 통해 또 다른 아미노산에 각각 가교결합된 2개의 펜테닐-알라닌 올레핀 측쇄를 포함하는 아미노산이다.
예를 들면, SP-72, SP-56 및 SP-138로서 나타낸 화합물은 하기 구조를 갖는다(기재된 순서대로 각각 서열 번호 109, 93 및 173):
Figure pat00049
예를 들면, 추가 화합물은 하기 구조를 갖는다(기재된 순서대로 각각 서열 번호 83, 177, 303, 163, 225, 273, 366, 217, 214, 387 및 184):
Figure pat00050
Figure pat00051
Figure pat00052
실시예 3: 경쟁 결합 ELISA(HDM2 및 HDMX)
실온에서 p53-His6(서열 번호 796으로서 개시된 "His6") 단백질(30 nM/웰)을 96웰 임뮬론(Immulon) 플레이트의 웰 내에 밤새 코팅한다. 실험 당일, 자동화된 ELISA 플레이트 세척기를 이용하여 플레이트를 1X PBS-트윈(Tween) 20(0.05%)으로 세척하고 실온에서 ELISA 마이크로 웰 차단제로 30분 동안 차단하고, 플레이트를 1X PBS-트윈 20(0.05%)으로 세척하여 과량의 차단제를 세척한다. 펩티드를 10 mM DMSO 저장액으로부터 멸균수 중의 500 μM 작업 저장액까지 희석하고, DMSO의 농도가 샘플들 사이에 일정하게 유지되도록 0.5% DMSO로 더 희석한다. 펩티드를 50 ㎕ 부피의 2X 원하는 농도로 웰에 첨가한 후, 희석된 GST-HDM2 또는 GST-HMDX 단백질(최종 농도: 10 nM)을 첨가한다. 샘플을 실온에서 2시간 동안 인큐베이션하고, 플레이트를 PBS-트윈 20(0.05%)으로 세척한 후, HRP 안정화 완충제 중에 0.5 ㎍/㎖까지 희석된 100 ㎕의 HRP 접합된 항-GST 항체[하이프로매트릭스 인코포레이티드(Hypromatrix, INC)]를 첨가한다. 검출 항체와 함께 30분 동안 인큐베이션한 후, 플레이트를 세척하고 웰당 100 ㎕의 TMB-E 기질 용액과 함께 30분 이하 동안 인큐베이션하고, 1 M HCL을 사용하여 반응을 중단시키고, 마이크로플레이트 판독기 상에서 450 nm에서 흡광도를 측정한다. 그래프패드 프리즘 소프트웨어를 이용하여 데이터를 분석한다.
실시예 4: SJSA-1 세포 생존능 분석
분석 전날, SJSA-1 세포를 5000개 세포/100 ㎕/웰의 밀도로 96웰 플레이트에 시딩한다. 연구 당일, 세포를 옵티(Opti)-MEM 배지로 1회 세척하고, 90 ㎕의 옵티-MEM 배지를 세포에 첨가한다. 펩티드를 10 mM DMSO 저장액으로부터 멸균수 중의 500 μM 작업 저장액까지 희석하고, DMSO의 농도가 샘플들 사이에 일정하게 유지되도록 0.5% DMSO로 더 희석한다. 펩티드에 대한 최종 농도 범위(μM)는 웰 당 100 ㎕ 최종 부피의 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.1 μM, 1.56 μM, 0.8 μM 및 0 μM일 것이다. 가장 높은 최종 DMSO 농도는 0.5%이고 음성 대조군으로서 사용될 것이다. 케이맨 케미칼스(Cayman Chemicals)의 세포에 기초한 분석 (-)-뉴틀린-3(10 mM)이 양성 대조군으로서 사용된다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석하였다. 10 ㎕의 10X 원하는 농도를 적절한 웰에 첨가하여 최종 원하는 농도를 달성한다. 그 다음, 습한 5% CO2 대기 하에 37℃에서 세포를 펩티드와 함께 20시간 내지 24시간 동안 인큐베이션한다. 인큐베이션 시간 후, 프로메가 세포 타이터-글로 시약을 제조자의 지시에 따라 사용하여 세포 생존능을 측정한다.
실시예 5: SJSA-1 p21 상향조절 분석
분석 전날, SJSA-1 세포를 80만개 세포/2 ㎖/웰의 밀도로 6웰 플레이트에 시딩한다. 연구 당일, 세포를 옵티-MEM 배지로 1회 세척하고, 1350 ㎕의 옵티-MEM 배지를 세포에 첨가한다. 펩티드를 10 mM DMSO 저장액으로부터 멸균수 중의 500 μM 작업 저장액까지 희석하고, DMSO의 농도가 샘플들 사이에 일정하게 유지되도록 0.5% DMSO로 더 희석한다. 가장 높은 최종 DMSO 농도는 0.5%이고 음성 대조군으로서 사용된다. 케이맨 케미칼스의 세포에 기초한 분석 (-)-뉴틀린-3(10 mM)이 양성 대조군으로서 사용된다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석한다. 150 ㎕의 10X 원하는 농도를 적절한 웰에 첨가하여 최종 원하는 농도를 달성한다. 그 다음, 습한 5% CO2 대기 하에 37℃에서 세포를 펩티드와 함께 18시간 내지 20시간 동안 인큐베이션한다. 인큐베이션 시간 후, 세포를 수거하여 1X PBS(Ca++/Mg++ 무함유)로 세척하고 얼음 상에서 1X 세포 용해 완충제(1X까지 희석되고 프로테아제 억제제 및 포스파타제 억제제로 보충된 셀 시그날링 테크놀로지스(Cell Signaling technologies) 10X 완충제)로 30분 동안 용해시킨다. 용해물을 4℃에서 미세원심분리기 내에서 13000 rpm 속도로 8분 동안 원심분리하고, 투명한 상청액을 수집하고 추가 사용 시까지 -80℃에서 저장한다. 써모피셔(Thermofisher)로부터의 BCA 단백질 검출 키트 및 BSA 표준물을 사용하여 상기 용해물의 총 단백질 함량을 측정한다. p21 검출 ELISA 분석을 위해 25 ㎍의 총 단백질을 사용한다. ELISA 플레이트에 대한 각각의 조건을 삼중으로 설정한다. 제조자의 지시에 따라 ELISA 분석 프로토콜을 따른다. 각각의 웰에 대해 25 ㎍의 총 단백질을 사용하고, 각각의 웰을 삼중으로 준비한다. 그래프패드 프리즘 소프트웨어를 이용하여 데이터를 분석한다.
실시예 6: p53 GRIP 분석
써모 사이언티픽* 바이오이미지(Thermo Scientific* BioImage) p53-Hdm2 재분포 분석은 약물 화합물 또는 다른 자극에 반응하여 일어나는 Hdm2와의 단백질 상호작용 및 GFP 태그 표지된 p53의 세포 전위(translocation)를 모니터링한다. 재조합 CHO-hIR 세포는 향상된 녹색 형광 단백질(EGFP) 및 PDE4A4-Hdm2(1-124)(PDE4A4와 Hdm2(1-124) 사이의 융합 단백질)의 C 말단에 융합된 인간 p53(1-312)을 안정하게 발현한다. 상기 세포는 p53과 Hdm2의 상호작용에 대한 실험 조건의 효과를 측정하기 위한 사용 준비된 분석 시스템을 제공한다. HCS 플랫폼을 이용하여 영상화 및 분석을 수행한다.
CHO-hIR 세포를 1%의 페니실린-스트렙토마이신, 0.5 ㎎/㎖의 제네티신, 1 ㎎/㎖의 제오신 및 10% FBS로 보충된 햄(Ham)의 F12 배지에서 정기적으로 유지한다. 배양 배지를 사용하는 분석을 실시하기 18시간 내지 24시간 전, 세포를 웰 당 7000개 세포/100 ㎕의 밀도로 96웰 플레이트에 시딩한다. 다음 날, 배지를 새로 공급하고, PD177을 3 μM의 최종 농도로 세포에 첨가하여 초점 형성을 활성화시킨다. 대조군 웰은 PD-177 용액의 사용 없이 유지된다. PD177을 사용한 자극으로부터 24시간 후, 세포를 옵티-MEM 배지로 1회 세척하고, PD-177(6 μM)로 보충된 50 ㎕의 옵티-MEM 배지를 세포에 첨가한다. 펩티드를 10 mM DMSO 저장액으로부터 멸균수 중의 500 μM 작업 저장액까지 희석하고, DMSO의 농도가 샘플들 사이에 일정하게 유지되도록 0.5% DMSO로 더 희석한다. 가장 높은 최종 DMSO 농도는 0.5%이고 음성 대조군으로서 사용된다. 케이맨 케미칼스의 세포에 기초한 분석 (-)-뉴틀린-3(10 mM)이 양성 대조군으로서 사용된다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석하였다. 50 ㎕의 2X 원하는 농도를 적절한 웰에 첨가하여 최종 원하는 농도를 달성한다. 그 다음, 습한 5% CO2 대기 하에 37℃에서 세포를 펩티드와 함께 6시간 동안 인큐베이션한다. 인큐베이션 시간 후, 배지를 약하게 흡인하고 실온에서 웰 당 150 ㎕의 고정 용액을 20분 동안 첨가하여 세포를 고정시킨다. 고정된 세포를 매번 웰 당 200 ㎕의 PBS로 4회 세척한다. 마지막 세척의 말기에, 100 ㎕의 1 μM 훼히스트(Hoechst) 염색 용액을 첨가한다. 밀봉된 플레이트를 암실 내에서 30분 이상 동안 인큐베이션하고 PBS로 세척하여 과량의 염료를 제거하고, PBS를 각각의 웰에 첨가한다. 플레이트를 4℃의 암실 내에서 3일 이하 동안 저장할 수 있다. 10X 대물렌즈, 및 훼히스트 및 GFP에 대한 XF-100 필터 세트를 이용하는 셀로믹스 어레이스캔(Cellomics Arrayscan) 기계 상에서 분자 전위 모듈(module)을 이용하여 p53/HDM2의 전위를 영상화한다. 출력 파라미터는 평균-서크링아베인텐레티오(CircRINGAveIntenRatio)(핵 및 세포질의 평균 형광 강도의 비, (웰 평균))이었다. 영상 분석을 위해 사용된 웰 당 최소한으로 허용가능한 수의 세포를 500개 세포로 설정하였다.
실시예 7: 형광 편광(FP)을 이용한 직접적 결합 분석 hDM2
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
1. hDM2(사내 제조, 41 kD)를 FP 완충제(고 농도 염 완충제 - 200 mM NaCl, 5 mM CHAPS, pH 7.5)로 희석하여 10 μM 작업 저장 용액을 제조한다.
2. 30 ㎕의 10 μM 단백질 저장 용액을 96웰 흑색 HE 마이크로플레이트(몰레큘라 디바이시스)의 A1 및 B1 웰에 첨가한다.
3. 30 ㎕의 FP 완충제를 A2 내지 A12, B2 내지 B12, C1 내지 C12 및 D1 내지 D12 열에 충전시킨다.
4. 마지막 희석 점에서 단일 숫자 nM 농도에 도달하도록 A1 및 B1로부터 A2 및 B2로, A2 및 B2로부터 A3 및 B3으로, . . . 단백질 저장 용액의 2배 또는 3배 연속 희석물을 제조한다.
5. (100% DMSO 중의) 1 mM의 FAM 표지된 선형 펩티드를 100 μM까지 DMSO로 희석한다(희석 1:10). 그 다음, 100 μM로부터 10 μM까지 물로 희석한 후(희석 1:10), 10 μM로부터 40 nM까지 FP 완충제로 희석한다(희석 1:250). 이것은 웰에서 10 nM 농도가 될 작업 용액이다(희석 1:4). 희석된 FAM 표지된 펩티드를 사용할 때까지 암실 내에서 유지한다.
6. 10 ㎕의 10 nM FAM 표지된 펩티드를 각각의 웰에 첨가하고 인큐베이션하고 상이한 시점에 판독한다. 5-FAM-BaLTFEHYWAQLTS-NH2(서열 번호 795)를 사용하여 얻은 Kd는 약 13.38 nM이다.
실시예 8: hDM2에 대한 경쟁 형광 편광 분석
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
1. hDM2(사내 제조, 41 kD)를 FP 완충제(고 농도 염 완충제 - 200 mM NaCl, 5 mM CHAPS, pH 7.5)로 희석하여 84 nM(2X) 작업 저장 용액을 제조한다.
2. 20 ㎕의 84 nM(2X) 단백질 저장 용액을 96웰 흑색 HE 마이크로플레이트(몰레큘라 디바이시스)의 각각의 웰에 첨가한다.
3. (100% DMSO 중의) 1 mM의 FAM 표지된 선형 펩티드를 100 μM까지 DMSO로 희석한다(희석 1:10). 그 다음, 100 μM로부터 10 μM까지 물로 희석한 후(희석 1:10), 10 μM로부터 40 nM까지 FP 완충제로 희석한다(희석 1:250). 이것은 웰에서 10 nM 농도가 될 작업 용액이다(희석 1:4). 희석된 FAM 표지된 펩티드를 사용할 때까지 암실 내에서 유지한다.
4. 1 μM(최종)의 펩티드로 출발하여 하기 희석 방식을 이용하여 6개의 점에 대해 5배 연속 희석물을 제조함으로써 비표지된 펩티드 투여량 플레이트를 FP 완충제로 제조한다:
(100% DMSO 중의) 10 mM을 5 mM까지 DMSO로 희석한다(희석 1:2). 그 다음, 5 mM로부터 500 μM까지 H2O로 희석한 후(희석 1:10), 500 μM로부터 20 μM까지 FP 완충제로 희석한다(희석 1:25). 6개의 점에 대해 4 μM(4X)로부터 5배 연속 희석물을 제조한다.
5. 10 ㎕의 연속 희석된 비표지된 펩티드를 20 ㎕의 84 nM 단백질로 충전된 각각의 웰로 옮긴다.
6. 10 ㎕의 10 nM(4X) FAM 표지된 펩티드를 각각의 웰에 첨가하고 판독하기 위해 3시간 동안 인큐베이션한다.
실시예 7 및 8의 결과는 도 7a 내지 7d에서 HDM2 데이터로 제공되어 있다.
실시예 9: 형광 편광(FP)을 이용한 직접적 결합 분석 hDMX
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
1. hDMX(사내 제조, 40 kD)를 FP 완충제(고 농도 염 완충제 - 200 mM NaCl, 5 mM CHAPS, pH 7.5)로 희석하여 10 μM 작업 저장 용액을 제조한다.
2. 30 ㎕의 10 μM 단백질 저장 용액을 96웰 흑색 HE 마이크로플레이트(몰레큘라 디바이시스)의 A1 및 B1 웰에 첨가한다.
3. 30 ㎕의 FP 완충제를 A2 내지 A12, B2 내지 B12, C1 내지 C12 및 D1 내지 D12 열에 충전시킨다.
4. 마지막 희석 점에서 단일 숫자 nM 농도에 도달하도록 A1 및 B1로부터 A2 및 B2로, A2 및 B2로부터 A3 및 B3으로, . . . 단백질 저장 용액의 2배 또는 3배 연속 희석물을 제조한다.
5. (100% DMSO 중의) 1 mM의 FAM 표지된 선형 펩티드를 100 μM까지 DMSO로 희석한다(희석 1:10). 그 다음, 100 μM로부터 10 μM까지 물로 희석한 후(희석 1:10), 10 μM로부터 40 nM까지 FP 완충제로 희석한다(희석 1:250). 이것은 웰에서 10 nM 농도가 될 작업 용액이다(희석 1:4). 희석된 FAM 표지된 펩티드를 사용할 때까지 암실 내에서 유지한다.
6. 10 ㎕의 10 nM FAM 표지된 펩티드를 각각의 웰에 첨가하고 인큐베이션하고 상이한 시점에 판독한다. 5-FAM-BaLTFEHYWAQLTS-NH2(서열 번호 795)를 사용하여 얻은 Kd는 약 51 nM이다.
실시예 10: hDMX에 대한 경쟁 형광 편광 분석
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
1. hDMX(사내 제조, 40 kD)를 FP 완충제(고 농도 염 완충제 - 200 mM NaCl, 5 mM CHAPS, pH 7.5)로 희석하여 300 nM(2X) 작업 저장 용액을 제조한다.
2. 20 ㎕의 300 nM(2X) 단백질 저장 용액을 96웰 흑색 HE 마이크로플레이트(몰레큘라 디바이시스)의 각각의 웰에 첨가한다.
3. (100% DMSO 중의) 1 mM의 FAM 표지된 선형 펩티드를 100 μM까지 DMSO로 희석한다(희석 1:10). 그 다음, 100 μM로부터 10 μM까지 물로 희석한 후(희석 1:10), 10 μM로부터 40 nM까지 FP 완충제로 희석한다(희석 1:250). 이것은 웰에서 10 nM 농도가 될 작업 용액이다(희석 1:4). 희석된 FAM 표지된 펩티드를 사용할 때까지 암실 내에서 유지한다.
4. 5 μM(최종)의 펩티드로 출발하여 하기 희석 방식을 이용하여 6개의 점에 대해 5배 연속 희석물을 제조함으로써 비표지된 펩티드 투여량 플레이트를 FP 완충제로 제조한다.
5. (100% DMSO 중의) 10 mM을 5 mM까지 DMSO로 희석한다(희석 1:2). 그 다음, 5 mM로부터 500 μM까지 H2O로 희석한 후(희석 1:10), 500 μM로부터 20 μM까지 FP 완충제로 희석한다(희석 1:25). 6개의 점에 대해 20 μM(4X)로부터 5배 연속 희석물을 제조한다.
6. 10 ㎕의 연속적으로 희석된 비표지된 펩티드를 20 ㎕의 300 nM 단백질로 충전된 각각의 웰로 옮긴다.
7. 10 ㎕의 10 nM(4X) FAM 표지된 펩티드를 각각의 웰에 첨가하고 판독하기 위해 3시간 동안 인큐베이션한다.
실시예 9 및 10의 결과는 도 7a 내지 7d에서 HDMX 데이터로 제공되어 있다.
실시예 11: 세포 생존능 분석
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
세포 플레이팅: 분석 전날, 세포를 트립신으로 처리하고 세포의 수를 세고 세포를 미리 결정된 밀도로 96웰 플레이트에 시딩한다. 사용될 각각의 세포주에 대해 하기 세포 밀도가 이용된다:
ㆍ SJSA-1: 7500개 세포/웰
ㆍ RKO: 5000개 세포/웰
ㆍ RKO-E6: 5000개 세포/웰
ㆍ HCT-116: 5000개 세포/웰
ㆍ SW-480: 2000개 세포/웰
ㆍ MCF-7: 5000개 세포/웰
연구 당일, 실온에서 배지를 11% FBS를 갖는 새로운 배지(분석 배지)로 교체한다. 웰 당 180 ㎕의 상기 분석 배지를 첨가한다. 세포를 갖지 않는 대조군 웰은 200 ㎕의 배지를 제공받는다.
펩티드 희석: 모든 희석물들을 실온에서 제조하고 실온에서 세포에 첨가한다.
ㆍ DMSO 중의 10 mM 펩티드 저장액을 제조한다. 희석제로서 DMSO를 사용하여 10 mM, 3.3 mM, 1.1 mM, 0.33 mM, 0.11 mM, 0.03 mM 및 0.01 mM 용액을 수득하기 위해 1:3 희석 방식을 이용하여 상기 저장액을 연속적으로 희석한다. DMSO에 의해 연속적으로 희석된 펩티드를 멸균수를 사용하여 33.3배 희석한다. 이것은 10X 작업 저장액을 제공한다. 대조군 웰을 위해 DMSO/멸균수(3% DMSO) 혼합물도 제조한다.
ㆍ 따라서, 상기 작업 저장액 농도 범위(μM)는 300 μM, 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM 및 0 μM일 것이다. 멀티채널을 이용하여 각각의 희석 단계에서 잘 혼합한다.
ㆍ H 열은 대조군을 갖는다. H1 내지 H3은 20 ㎕의 분석 배지를 제공받을 것이다. H4 내지 H9는 20 ㎕의 3% DMSO-물 비히클을 제공받을 것이다. H10 내지 H12는 세포를 갖지 않는 대조군으로서 배지만을 가질 것이다.
ㆍ 양성 대조군: HDM2 소분자 억제제인 뉴틀린-3a(10 mM)를 양성 대조군으로서 사용한다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석하였다.
세포에의 작업 저장액의 첨가:
ㆍ 20 ㎕의 10X 원하는 농도를 적절한 웰에 첨가하여 웰에서 총 200 ㎕의 부피로 최종 농도를 달성한다(20 ㎕의 300 μM 펩티드 + 180 ㎕의 배지 중의 세포 = 웰에서 200 ㎕ 부피의 30 μM 최종 농도). 피펫을 이용하여 수회 약하게 혼합한다. 따라서, 사용된 최종 농도 범위는 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM, 0.1 μM, 0.03 μM 및 0 μM일 것이다(강력한 펩티드의 경우 추가 희석이 포함됨).
ㆍ 대조군은 펩티드를 갖지 않되 펩티드를 함유하는 웰과 동일한 농도의 DMSO를 함유하는 웰, 및 세포를 함유하지 않는 웰을 포함한다.
ㆍ 습한 5% CO2 대기 하에 37℃에서 72시간 동안 인큐베이션한다.
ㆍ 프로메가로부터의 MTT 시약을 사용하여 세포의 생존능을 측정한다. SJSA-1, RKO, RKO-E6 및 HCT-116 세포의 생존능을 3일째 날에 측정하고, MCF-7 세포의 생존능을 5일째 날에 측정하고, SW-480 세포의 생존능을 6일째 날에 측정한다. 지정된 인큐베이션 시간의 말기에, 플레이트의 온도가 실온이 되게 한다. 80 ㎕의 분석 배지를 각각의 웰로부터 제거한다. 15 ㎕의 해동된 MTT 시약을 각각의 웰에 첨가한다.
ㆍ 플레이트를 습한 5% CO2 대기 하에 37℃에서 2시간 동안 인큐베이션하고, 100 ㎕의 가용화 시약을 제조자의 프로토콜에 따라 첨가한다. 실온에서 1시간 동안 교반하면서 인큐베이션하고 시너지 바이오텍(Synergy Biotek) 멀티플레이트 판독기 상에서 570 nM에서의 흡광도를 판독한다.
ㆍ 그래프패드 프리즘 분석 수단을 이용하여 DMSO 대조군과 비교된 세포 생존능을 분석한다.
시약:
ㆍ 인비트로겐 세포 배양 배지
i. 세포 배양물로 처리된 팔콘 96웰 투명 플레이트(넌크 353072)
ㆍ DMSO(시그마 D 2650)
ㆍ RPMI 1640(인비트로겐 72400)
ㆍ MTT(프로메가 G4000)
기계: 흡광도 판독을 위한 멀티플레이트 판독기(시너지 2)
실시예 11의 결과는 도 7a 내지 7d에서 SJSA-1 EC50 데이터로 제공되어 있다.
실시예 12: P21 ELISA 분석
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
세포 플레이팅:
ㆍ 분석 전날, SJSA-1 세포를 트립신으로 처리하고 상기 세포의 수를 세고 상기 세포를 7500개 세포/100 ㎕/웰의 밀도로 96웰 플레이트에 시딩한다.
ㆍ 연구 당일, 배지를 새로운 RPMI-11% FBS(분석 배지)로 교체한다. 웰 당 90 ㎕의 상기 분석 배지를 첨가한다. 세포를 갖지 않는 대조군 웰은 100 ㎕의 배지를 제공받는다.
펩티드 희석:
ㆍ DMSO 중의 10 mM 펩티드 저장액을 제조한다. 희석제로서 DMSO를 사용하여 10 mM, 3.3 mM, 1.1 mM, 0.33 mM, 0.11 mM, 0.03 mM 및 0.01 mM 용액을 수득하기 위해 1:3 희석 방식을 이용하여 상기 저장액을 연속적으로 희석한다. DMSO에 의해 연속적으로 희석된 펩티드를 멸균수를 사용하여 33.3배 희석한다. 이것은 10X 작업 저장액을 제공한다. 대조군 웰을 위해 DMSO/멸균수(3% DMSO) 혼합물도 제조한다.
ㆍ 따라서, 상기 작업 저장액 농도 범위(μM)는 300 μM, 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM 및 0 μM일 것이다. 멀티채널을 이용하여 각각의 희석 단계에서 잘 혼합한다.
ㆍ H 열은 대조군을 갖는다. H1 내지 H3은 10 ㎕의 분석 배지를 제공받을 것이다. H4 내지 H9는 10 ㎕의 3% DMSO-물 비히클을 제공받을 것이다. H10 내지 H12는 세포를 갖지 않는 대조군으로서 배지만을 가질 것이다.
ㆍ 양성 대조군: HDM2 소분자 억제제인 뉴틀린-3a(10 mM)를 양성 대조군으로서 사용한다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석하였다.
세포에의 작업 저장액의 첨가:
ㆍ 10 ㎕의 10X 원하는 농도를 적절한 웰에 첨가하여 웰에서 총 100 ㎕의 부피로 최종 농도를 달성한다(10 ㎕의 300 μM 펩티드 + 90 ㎕의 배지 중의 세포 = 웰에서 100 ㎕ 부피의 30 μM 최종 농도). 따라서, 사용된 최종 농도 범위는 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM 및 0 μM일 것이다.
ㆍ 대조군은 펩티드를 갖지 않되 펩티드를 함유하는 웰과 동일한 농도의 DMSO를 함유하는 웰, 및 세포를 함유하지 않는 웰을 포함한다.
ㆍ 20시간 인큐베이션 후, 배지를 흡인하고, 세포를 1X PBS(Ca++/Mg++ 무함유)로 세척하고 얼음 상에서 60 ㎕의 1X 세포 용해 완충제(1X까지 희석되고 프로테아제 억제제 및 포스파타제 억제제로 보충된 셀 시그날링 테크놀로지스 10X 완충제)로 30분 동안 용해시킨다.
ㆍ 플레이트를 4℃에서 5000 rpm 속도로 8분 동안 원심분리하고, 투명한 상청액을 수집하고 추가 사용 시까지 -80℃에서 동결시킨다.
단백질 평가:
ㆍ 써모피셔로부터의 BCA 단백질 검출 키트 및 BSA 표준물을 사용하여 상기 용해물의 총 단백질 함량을 측정한다. 전형적으로 웰 당 약 6 ㎍ 내지 7 ㎍의 단백질이 예상된다.
ㆍ 웰 당 50 ㎕의 상기 용해물을 사용하여 p21 ELISA를 준비한다.
인간 총 p21 ELISA: 제조자의 지시에 따라 ELISA 분석 프로토콜을 따른다. 각각의 웰에 대해 50 ㎕의 용해물을 사용하고, 각각의 웰을 삼중으로 준비한다.
시약:
ㆍ 세포에 기초한 분석 (-)-뉴틀린-3(10 mM): 케이맨 케미칼스, 카탈로그 # 600034
ㆍ 옵티-MEM: 인비트로겐, 카탈로그 # 51985
ㆍ 세포 신호전달 용해 완충제(10X): 셀 시그날링 테크놀로지, 카탈로그 # 9803
ㆍ 프로테아제 억제제 칵테일 정제(미니): 로슈 케미칼스(Roche Chemicals), 카탈로그 # 04693124001
ㆍ 포스파타제 억제제 칵테일 정제: 로슈 케미칼스, 카탈로그 # 04906837001
ㆍ 인간 총 p21 ELISA 키트: 알앤드디 시스템스(R&D Systems), DYC1047-5
ㆍ 중단 용액(1 M HCL): 셀 시그날링 테크놀로지스, 카탈로그 # 7002
기계: 미세원심분리기 - 에펜도르프 5415D 및 흡광도 판독용 멀티플레이트 판독기(시너지 2)
실시예 12의 결과는 도 7a 내지 7d에서 p21 데이터로 제공되어 있다.
실시예 13: 캐스파제 3 검출 분석
하기 일반적인 프로토콜에 따라 분석을 수행하였다:
세포 플레이팅: 분석 전날, SJSA-1 세포를 트립신으로 처리하고 상기 세포의 수를 세고 상기 세포를 7500개 세포/100 ㎕/웰의 밀도로 96웰 플레이트에 시딩한다. 연구 당일, 배지를 새로운 RPMI-11% FBS(분석 배지)로 교체한다. 웰 당 180 ㎕의 상기 분석 배지를 첨가한다. 세포를 갖지 않는 대조군 웰은 200 ㎕의 배지를 제공받는다.
펩티드 희석:
ㆍ DMSO 중의 10 mM 펩티드 저장액을 제조한다. 희석제로서 DMSO를 사용하여 10 mM, 3.3 mM, 1.1 mM, 0.33 mM, 0.11 mM, 0.03 mM 및 0.01 mM 용액을 수득하기 위해 1:3 희석 방식을 이용하여 상기 저장액을 연속적으로 희석한다. DMSO에 의해 연속적으로 희석된 펩티드를 멸균수를 사용하여 33.3배 희석한다. 이것은 10X 작업 저장액을 제공한다. 대조군 웰을 위해 DMSO/멸균수(3% DMSO) 혼합물도 제조한다.
ㆍ 따라서, 상기 작업 저장액 농도 범위(μM)는 300 μM, 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM 및 0 μM일 것이다. 멀티채널을 이용하여 각각의 희석 단계에서 잘 혼합한다. 20 ㎕의 10X 작업 저장액을 적절한 웰에 첨가한다.
ㆍ H 열은 대조군을 갖는다. H1 내지 H3은 20 ㎕의 분석 배지를 제공받을 것이다. H4 내지 H9는 20 ㎕의 3% DMSO-물 비히클을 제공받을 것이다. H10 내지 H12는 세포를 갖지 않는 대조군으로서 배지만을 가질 것이다.
ㆍ 양성 대조군: HDM2 소분자 억제제인 뉴틀린-3a(10 mM)를 양성 대조군으로서 사용한다. 펩티드와 동일한 희석 방식을 이용하여 뉴틀린을 희석하였다.
세포에의 작업 저장액의 첨가:
ㆍ 10 ㎕의 10X 원하는 농도를 적절한 웰에 첨가하여 웰에서 총 100 ㎕의 부피로 최종 농도를 달성한다(10 ㎕의 300 μM 펩티드 + 90 ㎕의 배지 중의 세포 = 웰에서 100 ㎕ 부피의 30 μM 최종 농도). 따라서, 사용된 최종 농도 범위는 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM 및 0 μM일 것이다.
ㆍ 대조군은 펩티드를 갖지 않되 펩티드를 함유하는 웰과 동일한 농도의 DMSO를 함유하는 웰, 및 세포를 함유하지 않는 웰을 포함한다.
ㆍ 48시간 인큐베이션 후, 각각의 웰로부터 80 ㎕의 배지를 흡인하고, 웰 당 100 ㎕의 캐스파제 3/7 글로 분석 시약(프로메가 캐스파제 3/7 글로 분석 시스템, G8092)을 첨가하고 실온에서 약하게 진탕하면서 1시간 동안 인큐베이션한다.
ㆍ 시너지 바이오텍 멀티플레이트 판독기 상에서 발광도를 판독한다.
ㆍ 데이터를 DMSO로 처리된 세포와 비교된 캐스파제 3 활성화로서 분석한다.
실시예 13의 결과는 도 7a 내지 7d에서 p21 데이터로 제공되어 있다.
SEQUENCE LISTING <110> AILERON THERAPEUTICS, INC. <120> PEPTIDOMIMETIC MACROCYCLES <130> 35224-757.601 <140> PCT/US2011/047692 <141> 2011-08-13 <150> 61/374,163 <151> 2010-08-16 <150> 61/373,638 <151> 2010-08-13 <150> 61/373,701 <151> 2010-08-13 <160> 796 <170> PatentIn version 3.5 <210> 1 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 1 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 2 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 2 Xaa Gln Ser Gln Xaa Thr Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln 1 5 10 15 Asn <210> 3 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 3 Xaa Ser Gln Gln Xaa Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 4 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (7)..(7) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 4 Gln Ser Xaa Gln Thr Phe Xaa Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 5 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 5 Gln Ser Gln Xaa Thr Phe Ser Xaa Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 6 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 6 Gln Ser Gln Gln Xaa Phe Ser Asn Xaa Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 7 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (11)..(11) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 7 Gln Ser Gln Gln Thr Phe Xaa Asn Leu Trp Xaa Leu Leu Pro Gln Asn 1 5 10 15 <210> 8 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (12)..(12) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 8 Gln Ser Gln Gln Thr Phe Ser Xaa Leu Trp Arg Xaa Leu Pro Gln Asn 1 5 10 15 <210> 9 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (11)..(11) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (15)..(15) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 9 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Xaa Leu Leu Pro Xaa Asn 1 5 10 15 <210> 10 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (12)..(12) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (16)..(16) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 10 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Arg Xaa Leu Pro Gln Xaa 1 5 10 15 <210> 11 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 11 Xaa Gln Ser Gln Gln Thr Phe Xaa Asn Leu Trp Arg Leu Leu Pro Gln 1 5 10 15 Asn <210> 12 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 12 Xaa Ser Gln Gln Thr Phe Ser Xaa Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 13 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 13 Gln Xaa Gln Gln Thr Phe Ser Asn Xaa Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 14 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (11)..(11) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 14 Gln Ser Gln Xaa Thr Phe Ser Asn Leu Trp Xaa Leu Leu Pro Gln Asn 1 5 10 15 <210> 15 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (12)..(12) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 15 Gln Ser Gln Gln Xaa Phe Ser Asn Leu Trp Arg Xaa Leu Pro Gln Asn 1 5 10 15 <210> 16 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (14)..(14) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 16 Gln Ser Gln Gln Thr Phe Xaa Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 17 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (15)..(15) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 17 Gln Ser Gln Gln Thr Phe Ser Xaa Leu Trp Arg Leu Leu Pro Xaa Asn 1 5 10 15 <210> 18 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (16)..(16) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 18 Gln Ser Gln Gln Thr Phe Ser Asn Xaa Trp Arg Leu Leu Pro Gln Xaa 1 5 10 15 <210> 19 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 19 Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 20 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 20 Xaa Leu Thr Phe Xaa His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 21 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 21 Xaa Thr Phe Glu Xaa Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 22 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (6)..(6) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 22 Leu Xaa Phe Glu His Xaa Trp Ala Gln Leu Thr Ser 1 5 10 <210> 23 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 23 Leu Thr Phe Xaa His Tyr Trp Xaa Gln Leu Thr Ser 1 5 10 <210> 24 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 24 Leu Thr Phe Glu Xaa Tyr Trp Ala Xaa Leu Thr Ser 1 5 10 <210> 25 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (12)..(12) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 25 Leu Thr Phe Glu His Tyr Trp Xaa Gln Leu Thr Xaa 1 5 10 <210> 26 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (13)..(13) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 26 Leu Thr Phe Glu His Tyr Trp Ala Xaa Leu Thr Ser Xaa 1 5 10 <210> 27 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 27 Xaa Thr Phe Glu His Tyr Trp Xaa Gln Leu Thr Ser 1 5 10 <210> 28 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 28 Gln Xaa Phe Glu His Tyr Trp Ala Xaa Leu Thr Ser 1 5 10 <210> 29 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (11)..(11) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 29 Gln Thr Phe Xaa His Tyr Trp Ala Gln Leu Xaa Ser 1 5 10 <210> 30 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (12)..(12) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 30 Gln Thr Phe Glu Xaa Tyr Trp Ala Gln Leu Thr Xaa 1 5 10 <210> 31 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (6)..(6) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (13)..(13) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 31 Gln Thr Phe Glu His Xaa Trp Ala Gln Leu Thr Ser Xaa 1 5 10 <210> 32 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-aminoisobutyric acid, His or Asn <220> <221> MOD_RES <222> (5)..(5) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue, Gln or Leu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 32 Phe Met Xaa Tyr Trp Glu Xaa Leu 1 5 <210> 33 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (4)..(4) <223> 2-aminoisobutyric acid, His or Asn <220> <221> MOD_RES <222> (5)..(5) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (6)..(6) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (8)..(8) <223> Aminocyclopropane carboxylic acid residue, Gln or Leu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 33 Xaa Phe Met Xaa Xaa Trp Glu Xaa Leu 1 5 <210> 34 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (3)..(3) <223> 2-aminoisobutyric acid, His or Asn <220> <221> MOD_RES <222> (5)..(5) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (6)..(6) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue, Gln or Leu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 34 Phe Xaa Xaa Tyr Trp Xaa Xaa Leu 1 5 <210> 35 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (5)..(5) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 35 Phe Met Xaa Tyr Trp Glu Xaa Leu 1 5 <210> 36 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (4)..(4) <223> 2-aminoisobutyric acid, His or Asn <220> <221> MOD_RES <222> (6)..(6) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (8)..(8) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 36 Xaa Phe Met Xaa Tyr Trp Glu Xaa Leu 1 5 <210> 37 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Any cross-linked amino acid <220> <221> MOD_RES <222> (3)..(3) <223> 2-aminoisobutyric acid, His or Asn <220> <221> MOD_RES <222> (5)..(5) <223> 6-Cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue, Gln or Leu <220> <221> MOD_RES <222> (9)..(9) <223> Any cross-linked amino acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 37 Phe Xaa Xaa Tyr Trp Glu Xaa Leu Xaa 1 5 <210> 38 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 38 Leu Ser Gln Glu Thr Phe Ala Asp Leu Trp Lys Leu Leu Ala Glu Asn 1 5 10 15 <210> 39 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 39 Leu Ser Gln Glu Thr Phe Ala Asn Leu Trp Lys Leu Leu Ala Gln Asn 1 5 10 15 <210> 40 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 40 Leu Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 41 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 41 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Lys Leu Leu Ala Gln Asn 1 5 10 15 <210> 42 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 42 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 43 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 43 Gln Ser Gln Gln Thr Ala Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 44 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 44 Gln Xaa Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 45 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 45 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 46 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 46 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 47 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 47 Gln Ser Gln Xaa Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 48 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 48 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Ala Asn 1 5 10 15 <210> 49 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 49 Ala Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 50 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 50 Gln Ser Gln Gln Thr Phe Ala Ala Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 51 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 51 Gln Ser Gln Glu Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 52 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 52 Arg Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 53 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 53 Arg Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Glu Asn 1 5 10 15 <210> 54 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 54 Leu Ser Gln Glu Thr Phe Ser Asp Leu Trp Lys Leu Leu Pro Glu Asn 1 5 10 15 <210> 55 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 55 Gln Ser Gln Ala Thr Phe Ser Ala Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 56 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 56 Gln Ser Gln Gln Ala Phe Ser Asn Ala Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 57 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 57 Gln Ser Gln Gln Thr Ala Ser Asn Leu Ala Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 58 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 58 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Pro Gln Asn 1 5 10 15 <210> 59 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (13)..(13) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (17)..(17) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 59 Arg Thr Gln Ala Thr Phe Ala Asn Gln Trp Xaa Ala Leu Ala Thr Asn 1 5 10 15 Xaa Thr Arg <210> 60 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 60 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Arg Asn 1 5 10 15 <210> 61 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 61 Gln Ser Gln Arg Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 62 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 62 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 63 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 63 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 64 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 64 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 65 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 65 Gln Ser Gln Gln Thr Tyr Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 66 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 66 Arg Xaa Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 67 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 67 Met Pro Arg Phe Met Asp Tyr Trp Glu Gly Leu Asn 1 5 10 <210> 68 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 68 Arg Ser Gln Gln Arg Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 69 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 69 Gln Ser Gln Gln Arg Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 70 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 70 Arg Xaa Gln Gln Arg Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 71 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 71 Arg Ser Gln Gln Arg Phe Ala Asn Phe Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 72 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 72 Arg Ser Gln Gln Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 73 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 73 Arg Ser Gln Gln Thr Phe Ala Asn Leu Trp Gln Leu Leu Ala Gln Asn 1 5 10 15 <210> 74 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me-Leu <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 74 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Gln Leu Leu Ala Gln Asn 1 5 10 15 <210> 75 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me-Leu <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 75 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 76 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 76 Leu Pro Arg Phe Ala Asp Tyr Trp Glu Gly Leu Ala Gln Asn 1 5 10 <210> 77 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 77 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 78 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 78 Arg Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 <210> 79 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> 2-fluoro-Phe <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 79 Gln Ser Gln Gln Thr Phe Ala Asn Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 80 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> 3-fluoro-Phe <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 80 Gln Ser Gln Gln Thr Phe Ala Asn Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 81 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-hydrogen R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-hydrogen S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 81 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 82 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 82 Arg Ser Gln Gln Thr Ala Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 83 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 83 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 84 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term acetylated with heptanoic acid and connected to internal olefin amino acid by an all-carbon crosslinker comprising one double bond <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 84 Gln Ser Gln Ala Thr Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 85 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term acetylated with heptanoic acid and connected to internal olefin amino acid by an all-carbon crosslinker comprising one double bond <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 85 Gln Ser Gln Ala Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 86 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> 6cl-Trp <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 86 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 87 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Me6cl-D-Trp <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 87 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 88 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 88 Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 89 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 89 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 90 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 90 Leu Thr Phe Glu Ala Tyr Trp Ala Ala Leu Thr Ser 1 5 10 <210> 91 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in cis configuration <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in cis configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 91 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 92 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 92 Leu Thr Phe Ala His Tyr Trp Arg Gln Leu Ala Ser 1 5 10 <210> 93 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Two Pentyl-alanine olefin amino acid side chain, each of which is crosslinked to another amino acid as indicated <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 93 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 94 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 94 Gln Ser Gln Gln Thr Ala Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 95 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 95 Gln Ser Gln Gln Thr Ala Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 96 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 96 Ala Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 97 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 97 Ala Ser Gln Gln Ala Phe Ser Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 98 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 98 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 99 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 99 Gln Ser Gln Gln Ala Phe Ser Asn Ala Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 100 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 100 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 101 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 101 Gln Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Ala Leu Xaa Gln Asn 1 5 10 15 <210> 102 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (15)..(15) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 102 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Ala Leu Leu Ala Ala Asn 1 5 10 15 <210> 103 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 103 Ala Ser Gln Gln Ala Phe Ser Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 104 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 104 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 105 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 105 Gln Ser Gln Gln Ala Phe Ser Asn Ala Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 106 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 106 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 107 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 107 Gln Ser Gln Ala Thr Phe Ser Ala Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 108 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 108 Gln Ser Gln Ala Thr Phe Ser Ala Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 109 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (7)..(7) <223> Two pentenyl-alanine olefin side chains where one side chain is not crosslinked <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 109 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 110 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 110 Ala Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 111 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 111 Gln Ser Gln Ala Thr Phe Ser Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 112 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (15)..(15) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 112 Gln Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Leu Leu Ala Ala Asn 1 5 10 15 <210> 113 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Two Pentenyl-alanine olefin side chains, each of which is crosslinked to another amino acid as indicated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 113 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 114 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 114 Ala Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 115 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 115 Gln Ser Gln Ala Thr Phe Ser Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 116 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (15)..(15) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 116 Gln Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Leu Leu Ala Ala Asn 1 5 10 15 <210> 117 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (7)..(7) <223> Two pentenyl-alanine olefin side chains where one side chain is not crosslinked <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 117 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 118 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 118 Gln Ser Gln Gln Thr Phe Ser Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 119 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-terminus acetylated with heptanoic acid <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 119 Gln Ser Gln Ala Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 120 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 120 Ala Ser Gln Gln Thr Phe Ala Asn Leu Arg Trp Leu Leu Ala Gln Asn 1 5 10 15 <210> 121 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 121 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 15 <210> 122 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 122 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 15 <210> 123 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 123 Ala Ala Ala Arg Ala Ala Ala Ala Ala Ala Arg Ala Ala Ala Ala Ala 1 5 10 15 <210> 124 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 124 Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser Ala 1 5 10 <210> 125 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 125 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser Ala 1 5 10 <210> 126 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 126 Ala Ser Gln Gln Thr Phe Ser Asn Leu Trp Arg Leu Leu Pro Gln Asn 1 5 10 15 <210> 127 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Two Pentenyl-alanine olefin side chains, each of which is crosslinked to another amino acid as indicated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 127 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Xaa Gln Asn 1 5 10 15 <210> 128 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 128 Gln Ser Gln Gln Thr Phe Xaa Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 129 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 129 Gln Ser Gln Gln Thr Phe Leu Asn Leu Trp Arg Leu Leu Leu Gln Asn 1 5 10 15 <210> 130 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <400> 130 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 131 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (14)..(14) <223> Norleucine <220> <223> C-term NH2 <400> 131 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Leu Gln Asn 1 5 10 15 <210> 132 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 132 Gln Ser Gln Gln Thr Phe Xaa Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 133 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 133 Gln Ser Gln Gln Thr Phe Leu Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 134 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (10)..(10) <223> 6cl-Trp <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 134 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 135 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (10)..(10) <223> Me6cl-Trp <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 135 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 136 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Norleucine <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 136 Phe Leu Ala Tyr Trp Glu Ala Leu 1 5 <210> 137 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 137 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala 1 5 10 <210> 138 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 138 Phe Ala Ala Tyr Trp Gln Leu Leu Ala Ala 1 5 10 <210> 139 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 139 Leu Pro Arg Phe Ala Asn Tyr Trp Glu Leu Leu Ala Gln Asn 1 5 10 <210> 140 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 140 Leu Pro Arg Phe Ala Asp Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 141 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 141 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln 1 5 10 <210> 142 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 142 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala 1 5 10 <210> 143 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 143 Gln Ser Gln Gln Thr Phe Ala Asp Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 144 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 144 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Glu Asn 1 5 10 15 <210> 145 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 145 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asp 1 5 10 15 <210> 146 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 146 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Ser 1 5 10 15 <210> 147 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 147 Glu Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 148 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 148 Leu Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 <210> 149 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 149 Leu Arg Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 <210> 150 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 150 Gln Ser Gln Gln Thr Phe Ala Asn Trp Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 151 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 151 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln 1 5 10 15 <210> 152 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 152 Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 153 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 153 Leu Pro Arg Phe Ala Asn Trp Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 154 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 154 Leu Pro Arg Phe Ala Asn Trp Trp Arg Leu Leu Ala Ala 1 5 10 <210> 155 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 155 Thr Ser Phe Ala Glu Tyr Trp Asn Leu Leu Ser Pro 1 5 10 <210> 156 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 156 Gln Thr Phe Ala His Trp Trp Ser Gln Leu Ala Ser 1 5 10 <210> 157 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 157 Phe Met Ala Tyr Trp Glu Ala Leu 1 5 <210> 158 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 158 Gln Thr Phe Glu His Trp Trp Ser Gln Leu Leu Ser 1 5 10 <210> 159 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me-Trp <220> <221> MOD_RES <222> (13)..(13) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 159 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 160 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 160 Phe Met Xaa Tyr Trp Glu Xaa Leu 1 5 <210> 161 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Norleucine <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 161 Phe Leu Ala Tyr Trp Glu Ala Leu 1 5 <210> 162 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in cis configuration <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in cis configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 162 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala 1 5 <210> 163 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 163 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 164 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> 6cl-Trp <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 164 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 165 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 165 Ala Phe Ala Ala Ala Trp Ala Leu Ala Ala Ala 1 5 10 <210> 166 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 166 Thr Phe Ala Ala Ala Trp Arg Leu Ala Ala Gln 1 5 10 <210> 167 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Pr <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 167 Thr Phe Ala Ala Ala Trp Arg Leu Ala Ala Gln 1 5 10 <210> 168 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 168 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 169 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 169 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser Ala 1 5 10 <210> 170 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 170 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 171 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (8)..(8) <223> 6cl-Trp <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 171 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 172 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (7)..(7) <223> 6cl-Trp <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 172 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 173 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (7)..(7) <223> Two pentenyl-alanine olefin side chains, each of which is crosslinked to another amino acid as indicated via fully saturated hydrocarbon crosslinks <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-methyl S8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 173 Gln Ser Ala Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 174 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (7)..(7) <223> 6cl-Trp <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 174 Leu Thr Phe Ala His Tyr Trp Arg Gln Leu Ala Ser 1 5 10 <210> 175 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (10)..(10) <223> 6cl-Trp <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 175 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Arg Leu Leu Ala Gln Asn 1 5 10 15 <210> 176 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (8)..(8) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 176 Ala Ser Gln Gln Thr Phe Ser Ala Leu Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 177 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 177 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 178 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 178 Thr Ser Phe Ala Gln Tyr Trp Asn Leu Leu Ala Pro 1 5 10 <210> 179 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <223> C-term NH2 <400> 179 Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 180 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 180 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala 1 5 <210> 181 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 181 Ala Phe Ala Ala Ala Trp Ala Leu Ala Ala Ala 1 5 10 <210> 182 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 182 Thr Phe Ala Ala Ala Trp Arg Leu Ala Ala Gln 1 5 10 <210> 183 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Pr <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 183 Thr Phe Ala Ala Ala Trp Arg Leu Ala Ala Gln 1 5 10 <210> 184 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 184 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 185 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Beta-Ala <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (21)..(21) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 185 Cys Cys Pro Gly Cys Cys Ala Gln Ser Gln Gln Thr Phe Ala Asn Leu 1 5 10 15 Trp Arg Leu Leu Ala Gln Asn 20 <210> 186 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Beta-Ala <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (21)..(21) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 186 Cys Cys Pro Gly Cys Cys Ala Gln Ser Gln Gln Thr Ala Ala Asn Leu 1 5 10 15 Trp Arg Leu Leu Ala Gln Asn 20 <210> 187 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Beta-Ala <220> <221> MOD_RES <222> (8)..(8) <223> Norleucine <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (19)..(19) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 187 Cys Cys Pro Gly Cys Cys Ala Leu Pro Arg Phe Ala Asn Tyr Trp Arg 1 5 10 15 Leu Leu Ala Gln Asn 20 <210> 188 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 188 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 189 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 189 Phe Ala His Tyr Trp Arg Xaa Leu Ala 1 5 <210> 190 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 190 Gln Thr Phe Ala His Trp Trp Ser Gln Leu Ala Ser 1 5 10 <210> 191 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 191 Leu Thr Ala Ala His Tyr Trp Arg Gln Leu Ala Ser 1 5 10 <210> 192 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 192 Gln Ala Gln Gln Thr Phe Ser Asn Ala Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 193 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 193 Gln Ser Gln Gln Ala Phe Ser Asn Leu Trp Arg Ala Leu Xaa Gln Asn 1 5 10 15 <210> 194 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 194 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 195 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 195 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ser 1 5 10 <210> 196 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 196 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Gln 1 5 10 <210> 197 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 197 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ser 1 5 10 <210> 198 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 198 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ser 1 5 10 <210> 199 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 199 Phe Ala Ala Tyr Trp Glu Gln Leu Ala Ser 1 5 10 <210> 200 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 200 Phe Ala His Tyr Trp Glu Gln Leu Ala Ser 1 5 10 <210> 201 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 201 Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 202 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 202 Phe Ala His Tyr Trp Ala Xaa Leu Ala Ser 1 5 10 <210> 203 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 203 Phe Ala His Tyr Trp Arg Xaa Leu Ala Ser 1 5 10 <210> 204 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-hydrogen S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 204 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 205 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 205 Leu Pro Thr Phe Xaa Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 206 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 206 Thr Phe Ala Ala Ala Trp Arg Ala Leu Ala Gln 1 5 10 <210> 207 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 207 Thr Ser Phe Xaa His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 208 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 208 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 209 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 209 Leu Thr Phe Ala His Tyr Trp Ala Gln Ile Ala Ser 1 5 10 <210> 210 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 210 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 211 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 211 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 212 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 212 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Xaa 1 5 10 <210> 213 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Abu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 213 Leu Thr Phe Ala His Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 214 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 214 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 215 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 215 Leu Ala Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 216 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 216 Leu Ala Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 217 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 217 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 218 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 218 Leu Ala Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 219 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 219 Leu Thr Phe Ala Asn Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 220 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 220 Leu Thr Phe Ala Asn Leu Trp Ala Leu Leu Ala Ala 1 5 10 <210> 221 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 221 Leu Thr Phe Ala Ala Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 222 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 222 Leu Ala Phe Ala Asn Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 223 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 223 Leu Ala Phe Ala Ala Leu Trp Ala Leu Leu Ala Ala 1 5 10 <210> 224 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 224 Ala Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 225 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 225 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Ala 1 5 10 <210> 226 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 226 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Xaa 1 5 10 <210> 227 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 227 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Leu 1 5 10 <210> 228 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 228 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 229 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 229 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 230 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 230 Phe Ala Ala Tyr Trp Glu Xaa Ile Ala Ala 1 5 10 <210> 231 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 231 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 232 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me-Leu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 232 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala 1 5 10 <210> 233 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 233 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala 1 5 10 <210> 234 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 234 Phe Ala Ala Tyr Trp Gln Xaa Leu Ala Ala 1 5 10 <210> 235 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 235 Phe Ala Ala Tyr Trp Ala Xaa Leu Ala Ala 1 5 10 <210> 236 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Abu <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 236 Phe Ala Ala Tyr Trp Xaa Xaa Leu Ala Ala 1 5 10 <210> 237 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Norleucine <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 237 Phe Ala Ala Tyr Trp Leu Xaa Leu Ala Ala 1 5 10 <210> 238 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (3)..(3) <223> Abu <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 238 Phe Ala Xaa Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 239 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (3)..(3) <223> Norleucine <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 239 Phe Ala Leu Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 240 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term 5-FAM <220> <221> MOD_RES <222> (1)..(1) <223> Beta-Ala <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 240 Ala Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 241 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term 5-FAM <220> <221> MOD_RES <222> (1)..(1) <223> Beta-Ala <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 241 Ala Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 242 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 242 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 243 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Tert-butyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 243 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 244 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Adamantyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 244 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 245 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 245 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 246 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 246 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 247 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Aminocyclohexane carboxylic acid residue <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 247 Leu Thr Phe Ala His Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 248 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Aminocyclopentane carboxylic acid residue <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 248 Leu Thr Phe Ala His Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 249 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Homo-Phe <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 249 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 250 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Indanyl-Gly <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 250 Leu Thr Gly Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 251 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 251 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 252 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 252 Leu Thr Phe Ala His Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 253 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Indanyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 253 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 254 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 254 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 255 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclopentylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 255 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 256 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Homo-Tyr <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 256 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 257 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Homo-Leu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 257 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 258 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Tert-butyl-Gly <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 258 Phe Ala Ala Tyr Trp Glu Xaa Gly Ala Ala 1 5 10 <210> 259 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Adamantyl-Gly <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 259 Phe Ala Ala Tyr Trp Glu Xaa Gly Ala Ala 1 5 10 <210> 260 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Homo-Cyclohexylalanine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 260 Phe Ala Ala Tyr Trp Glu Xaa Ala Ala Ala 1 5 10 <210> 261 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 261 Phe Ala Ala Tyr Trp Glu Xaa Ala Ala Ala 1 5 10 <210> 262 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclohexane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 262 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 263 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopentane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 263 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 264 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Homo-Phe <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 264 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 265 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Indanyl-Gly <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 265 Gly Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 266 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 266 Phe Ala Ala Tyr Trp Glu Xaa Phe Ala Ala 1 5 10 <210> 267 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Indanyl-Gly <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 267 Phe Ala Ala Tyr Trp Glu Xaa Gly Ala Ala 1 5 10 <210> 268 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 268 Phe Ala Ala Tyr Trp Glu Xaa Ala Ala Ala 1 5 10 <210> 269 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (8)..(8) <223> Cyclopentylglycine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 269 Phe Ala Ala Tyr Trp Glu Xaa Gly Ala Ala 1 5 10 <210> 270 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (4)..(4) <223> Homo-Tyr <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 270 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 271 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 271 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Gln 1 5 10 <210> 272 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 272 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 273 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 273 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 274 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 274 Leu Ala Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ala 1 5 10 <210> 275 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 275 Leu Thr Phe Ala His Tyr Trp Ala Xaa Leu Ala Ser 1 5 10 <210> 276 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 276 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 277 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 277 Val Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 278 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 278 Phe Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 279 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 279 Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 280 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 280 Arg Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 281 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 281 Lys Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 282 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Norleucine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 282 Leu Leu Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 283 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 283 Leu Val Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 284 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 284 Leu Phe Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 285 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 285 Leu Trp Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 286 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 286 Leu Arg Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 287 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 287 Leu Lys Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 288 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 288 Leu Thr Phe Ala Leu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 289 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 289 Leu Thr Phe Ala Val Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 290 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 290 Leu Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 291 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 291 Leu Thr Phe Ala Trp Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 292 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 292 Leu Thr Phe Ala Arg Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 293 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 293 Leu Thr Phe Ala Lys Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 294 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 294 Leu Thr Phe Ala His Leu Trp Ala Gln Leu Ala Ser 1 5 10 <210> 295 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 295 Leu Thr Phe Ala His Val Trp Ala Gln Leu Ala Ser 1 5 10 <210> 296 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 296 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 297 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 297 Leu Thr Phe Ala His Trp Trp Ala Gln Leu Ala Ser 1 5 10 <210> 298 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 298 Leu Thr Phe Ala His Arg Trp Ala Gln Leu Ala Ser 1 5 10 <210> 299 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 299 Leu Thr Phe Ala His Lys Trp Ala Gln Leu Ala Ser 1 5 10 <210> 300 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 300 Leu Thr Phe Ala His Tyr Trp Leu Gln Leu Ala Ser 1 5 10 <210> 301 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 301 Leu Thr Phe Ala His Tyr Trp Val Gln Leu Ala Ser 1 5 10 <210> 302 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 302 Leu Thr Phe Ala His Tyr Trp Phe Gln Leu Ala Ser 1 5 10 <210> 303 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 303 Leu Thr Phe Ala His Tyr Trp Trp Gln Leu Ala Ser 1 5 10 <210> 304 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 304 Leu Thr Phe Ala His Tyr Trp Lys Gln Leu Ala Ser 1 5 10 <210> 305 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 305 Leu Thr Phe Ala His Tyr Trp Ala Leu Leu Ala Ser 1 5 10 <210> 306 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 306 Leu Thr Phe Ala His Tyr Trp Ala Val Leu Ala Ser 1 5 10 <210> 307 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 307 Leu Thr Phe Ala His Tyr Trp Ala Phe Leu Ala Ser 1 5 10 <210> 308 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 308 Leu Thr Phe Ala His Tyr Trp Ala Trp Leu Ala Ser 1 5 10 <210> 309 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 309 Leu Thr Phe Ala His Tyr Trp Ala Arg Leu Ala Ser 1 5 10 <210> 310 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 310 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Leu 1 5 10 <210> 311 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 311 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Val 1 5 10 <210> 312 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 312 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Phe 1 5 10 <210> 313 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 313 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Trp 1 5 10 <210> 314 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 314 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Arg 1 5 10 <210> 315 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 315 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Lys 1 5 10 <210> 316 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 316 Gln Ala Gln Gln Thr Phe Ser Asn Ala Trp Arg Leu Leu Xaa Gln Asn 1 5 10 15 <210> 317 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 317 Gln Ser Gln Gln Ala Phe Ser Asn Leu Trp Arg Ala Leu Xaa Gln Asn 1 5 10 15 <210> 318 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Pyridylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 318 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 319 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 3-Pyridylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 319 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 320 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 4-Pyridylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 320 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 321 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2CF3 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 321 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 322 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2CN <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 322 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 323 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2Me <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 323 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 324 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 324 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 325 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4CF3 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 325 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 326 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4tBu <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 326 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 327 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-5F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 327 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 328 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 3Bth-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 328 Leu Thr Phe Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 329 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2Br <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 329 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 330 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4Br <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 330 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 331 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 331 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 332 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 332 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 333 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3CN <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 333 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 334 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4CN <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 334 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 335 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-34Cl2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 335 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 336 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-34F2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 336 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 337 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-35F2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 337 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 338 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 3,3-diphenyl-Ala <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 338 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 339 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 339 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 340 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 340 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 341 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 341 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 342 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4I <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 342 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 343 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Me <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 343 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 344 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4Me <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 344 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 345 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 345 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 346 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 346 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 347 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3CF3 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 347 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 348 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4NO2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 348 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 349 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3NO2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 349 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 350 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 2Thi <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 350 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 351 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Biphenylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 351 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 352 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4tBu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 352 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 353 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4CF3 <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 353 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 354 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4Cl <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 354 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 355 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4Me <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 355 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 356 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4Br <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 356 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 357 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4CN <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 357 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 358 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Phe-4NO2 <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 358 Leu Thr Phe Ala His Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 359 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 359 Leu Thr Phe Ala His Ala Trp Ala Gln Leu Ala Ser 1 5 10 <210> 360 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 360 Leu Thr Phe Ala His Ala Trp Ala Gln Leu Ala Ser 1 5 10 <210> 361 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 361 Leu Thr Phe Ala His Trp Ala Gln Leu Ala Ser 1 5 10 <210> 362 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 362 Leu Thr Phe Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 363 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 363 Leu Thr Phe Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 364 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 364 Leu Thr Phe Ala His Tyr Trp Ala Gln Ile Ala Ser 1 5 10 <210> 365 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 365 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 366 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker comprising one double bond in trans configuration <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 366 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 367 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 367 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Xaa 1 5 10 <210> 368 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Abu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 368 Leu Thr Phe Ala His Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 369 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 369 Leu Ala Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 370 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 370 Leu Thr Phe Ala Asn Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 371 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 371 Leu Thr Phe Ala Ala Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 372 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 372 Leu Ala Phe Ala Asn Leu Trp Ala Leu Leu Ala Gln 1 5 10 <210> 373 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 373 Phe Ala Ala Tyr Trp Ala Xaa Leu Ala Ala 1 5 10 <210> 374 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 374 Leu Thr Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ser 1 5 10 <210> 375 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 375 Leu Val Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 376 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Abu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 376 Leu Thr Phe Ala Ala Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 377 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 377 Leu Thr Phe Ala Ala Tyr Trp Leu Gln Leu Ala Ser 1 5 10 <210> 378 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> Abu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 378 Leu Thr Phe Ala Xaa Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 379 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 379 Leu Thr Phe Ala Ala Tyr Trp His Gln Leu Ala Ser 1 5 10 <210> 380 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 380 Leu Thr Phe Ala Ala Tyr Trp Lys Gln Leu Ala Ser 1 5 10 <210> 381 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Ornithine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 381 Leu Thr Phe Ala Ala Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 382 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 382 Leu Thr Phe Ala Ala Tyr Trp Arg Gln Leu Ala Ser 1 5 10 <210> 383 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 383 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 384 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 384 Leu Thr Phe Ala Ala Tyr Trp Arg Ala Leu Ala Ser 1 5 10 <210> 385 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 385 Leu Thr Phe Ala Ala Tyr Trp Arg Gln Leu Ala Ala 1 5 10 <210> 386 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 386 Leu Thr Phe Ala Ala Tyr Trp Arg Ala Leu Ala Ala 1 5 10 <210> 387 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 387 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 388 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 388 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 389 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 389 Leu Thr Phe Ala His Tyr Trp Ala Ala Leu Ala Ser 1 5 10 <210> 390 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Abu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 390 Leu Thr Phe Ala His Tyr Trp Ala Xaa Leu Ala Ser 1 5 10 <210> 391 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 391 Leu Thr Phe Ala His Tyr Trp Ala Leu Leu Ala Ser 1 5 10 <210> 392 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 392 Phe Ala Ala Tyr Trp His Ala Leu Ala Ala 1 5 10 <210> 393 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 393 Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ala 1 5 10 <210> 394 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 394 Phe Ala Ala Tyr Trp Ser Ala Leu Ala Ala 1 5 10 <210> 395 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 395 Phe Ala Ala Tyr Trp Arg Ala Leu Ala Ala 1 5 10 <210> 396 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 396 Phe Ala Ala Tyr Trp Lys Ala Leu Ala Ala 1 5 10 <210> 397 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Ornithine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 397 Phe Ala Ala Tyr Trp Xaa Ala Leu Ala Ala 1 5 10 <210> 398 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 398 Phe Ala Val Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 399 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 399 Phe Ala Phe Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 400 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 400 Phe Ala Trp Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 401 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 401 Phe Ala Arg Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 402 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 402 Phe Ala Lys Tyr Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 403 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (4)..(4) <223> Norleucine <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 403 Phe Ala Ala Leu Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 404 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 404 Phe Ala Ala Val Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 405 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 405 Phe Ala Ala Phe Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 406 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 406 Phe Ala Ala Trp Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 407 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 407 Phe Ala Ala Arg Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 408 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 408 Phe Ala Ala Lys Trp Glu Xaa Leu Ala Ala 1 5 10 <210> 409 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 409 Phe Ala Ala Tyr Trp Val Xaa Leu Ala Ala 1 5 10 <210> 410 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 410 Phe Ala Ala Tyr Trp Phe Xaa Leu Ala Ala 1 5 10 <210> 411 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 411 Phe Ala Ala Tyr Trp Trp Xaa Leu Ala Ala 1 5 10 <210> 412 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 412 Phe Ala Ala Tyr Trp Arg Xaa Leu Ala Ala 1 5 10 <210> 413 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 413 Phe Ala Ala Tyr Trp Lys Xaa Leu Ala Ala 1 5 10 <210> 414 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 414 Phe Ala Ala Tyr Trp Glu Val Leu Ala Ala 1 5 10 <210> 415 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 415 Phe Ala Ala Tyr Trp Glu Phe Leu Ala Ala 1 5 10 <210> 416 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 416 Phe Ala Ala Tyr Trp Glu Trp Leu Ala Ala 1 5 10 <210> 417 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 417 Phe Ala Ala Tyr Trp Glu Arg Leu Ala Ala 1 5 10 <210> 418 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 418 Phe Ala Ala Tyr Trp Glu Lys Leu Ala Ala 1 5 10 <210> 419 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 419 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Val 1 5 10 <210> 420 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 420 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Phe 1 5 10 <210> 421 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 421 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Trp 1 5 10 <210> 422 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 422 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Arg 1 5 10 <210> 423 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 423 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Lys 1 5 10 <210> 424 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 424 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Val 1 5 10 <210> 425 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 425 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Phe 1 5 10 <210> 426 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 426 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Trp 1 5 10 <210> 427 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 427 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Arg 1 5 10 <210> 428 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 428 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Lys 1 5 10 <210> 429 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 429 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala His 1 5 10 <210> 430 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2NO2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 430 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 431 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 431 Leu Thr Ala Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 432 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 432 Leu Thr Phe Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 433 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 433 Gln Ser Gln Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Ala Ala Asn 1 5 10 15 <210> 434 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 434 Gln Xaa Gln Gln Thr Phe Ala Asn Leu Trp Ala Leu Leu Ala Ala Asn 1 5 10 15 <210> 435 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 435 Gln Xaa Gln Gln Thr Phe Ala Ala Leu Trp Ala Leu Leu Ala Ala Asn 1 5 10 15 <210> 436 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (14)..(14) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 436 Ala Ala Ala Ala Thr Phe Ala Ala Ala Trp Ala Ala Leu Ala Ala Ala 1 5 10 15 <210> 437 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 437 Phe Ala Ala Ala Trp Arg Ala Leu Ala Gln 1 5 10 <210> 438 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 438 Thr Phe Ala Ala Ala Trp Ala Ala Leu Ala Gln 1 5 10 <210> 439 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 439 Thr Phe Ala Ala Ala Trp Arg Ala Leu Ala Ala 1 5 10 <210> 440 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 440 Val Phe Ala Ala Ala Trp Arg Ala Leu Ala Gln 1 5 10 <210> 441 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 441 Ala Phe Ala Ala Ala Trp Ala Ala Leu Ala Ala 1 5 10 <210> 442 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 442 Thr Phe Ala Ala Ala Trp Lys Ala Leu Ala Gln 1 5 10 <210> 443 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Ornithine <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 443 Thr Phe Ala Ala Ala Trp Xaa Ala Leu Ala Gln 1 5 10 <210> 444 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 444 Thr Phe Ala Ala Ala Trp Ser Ala Leu Ala Gln 1 5 10 <210> 445 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 445 Leu Thr Phe Ala Ala Ala Trp Arg Ala Leu Ala Gln 1 5 10 <210> 446 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 446 Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 447 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 447 Phe Ala Ala Trp Trp Ala Ala Leu Ala Ala 1 5 10 <210> 448 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 448 Phe Ala Ala Trp Trp Ala Gln Leu Ala Ala 1 5 10 <210> 449 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 449 Phe Ala Ala Tyr Trp Glu Ala Leu Ala 1 5 <210> 450 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 450 Phe Ala Ala Tyr Trp Ala Ala Leu Ala 1 5 <210> 451 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 451 Phe Ala Ala Tyr Trp Lys Ala Leu Ala 1 5 <210> 452 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Ornithine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 452 Phe Ala Ala Tyr Trp Xaa Ala Leu Ala 1 5 <210> 453 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 453 Phe Ala Ala Tyr Trp Gln Ala Leu Ala 1 5 <210> 454 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 454 Phe Ala Ala Tyr Trp Ala Gln Leu Ala 1 5 <210> 455 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 455 Phe Ala His Tyr Trp Asp Gln Leu Ala Ser 1 5 10 <210> 456 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 456 Phe Ala His Phe Trp Glu Gln Leu Ala Ser 1 5 10 <210> 457 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 457 Phe Ala Ala Tyr Trp His Gln Leu Ala Ser 1 5 10 <210> 458 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 458 Phe Ala Ala Tyr Trp Lys Gln Leu Ala Ser 1 5 10 <210> 459 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Ornithine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 459 Phe Ala Ala Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 460 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 460 Phe Ala His Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 461 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 461 Phe Ala His Trp Trp Glu Gln Leu Ala Ser 1 5 10 <210> 462 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 462 Phe Ala His Trp Trp Ala Gln Leu Ala Ser 1 5 10 <210> 463 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 463 Phe Ala Ala Trp Trp His Gln Leu Ala Ser 1 5 10 <210> 464 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 464 Phe Ala Ala Trp Trp Lys Gln Leu Ala Ser 1 5 10 <210> 465 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Ornithine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 465 Phe Ala Ala Trp Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 466 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 466 Phe Ala His Trp Trp Ser Gln Leu Ala Ser 1 5 10 <210> 467 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 467 Leu Thr Phe Ala Asn Tyr Trp Ala Leu Leu Ala Gln 1 5 10 <210> 468 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 468 Leu Thr Phe Ala Asn Leu Trp Ala Gln Leu Ala Gln 1 5 10 <210> 469 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 469 Leu Thr Phe Ala Asn Tyr Trp Ala Leu Leu Ala Ala 1 5 10 <210> 470 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 470 Leu Thr Phe Ala Asn Leu Trp Ala Gln Leu Ala Ala 1 5 10 <210> 471 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 471 Leu Thr Phe Ala Ala Tyr Trp Ala Leu Leu Ala Gln 1 5 10 <210> 472 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 472 Leu Thr Phe Ala Ala Leu Trp Ala Gln Leu Ala Gln 1 5 10 <210> 473 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 473 Leu Ala Phe Ala Asn Tyr Trp Ala Leu Leu Ala Gln 1 5 10 <210> 474 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 474 Leu Ala Phe Ala Asn Leu Trp Ala Gln Leu Ala Gln 1 5 10 <210> 475 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 475 Leu Ala Phe Ala Ala Tyr Trp Ala Leu Leu Ala Ala 1 5 10 <210> 476 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 476 Leu Ala Phe Ala Ala Leu Trp Ala Gln Leu Ala Ala 1 5 10 <210> 477 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 477 Leu Ala Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ala 1 5 10 <210> 478 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> 2-aminoisobutyric acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 478 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Xaa 1 5 10 <210> 479 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 479 Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala Ala 1 5 10 <210> 480 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 480 Phe Ala Ala Tyr Trp Ala Xaa Leu Ala Ala Ala 1 5 10 <210> 481 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 481 Phe Ala Ala Tyr Trp Ser Xaa Leu Ala Ala Ala 1 5 10 <210> 482 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 482 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Ser 1 5 10 <210> 483 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 483 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Thr 1 5 10 <210> 484 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 484 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Leu 1 5 10 <210> 485 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 485 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ala Gln 1 5 10 <210> 486 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 486 Phe Ala Ala Phe Trp Glu Xaa Leu Ala Ala Ala 1 5 10 <210> 487 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 487 Phe Ala Ala Trp Trp Glu Xaa Leu Ala Ala Ala 1 5 10 <210> 488 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 488 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Ser Ala 1 5 10 <210> 489 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 489 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 490 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 490 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala Ala 1 5 10 <210> 491 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 491 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Xaa Ala 1 5 10 <210> 492 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 492 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Leu Ala 1 5 10 <210> 493 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 493 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Leu Ala 1 5 10 <210> 494 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 494 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Leu Ala 1 5 10 <210> 495 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 495 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Leu Ala 1 5 10 <210> 496 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 496 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Xaa 1 5 10 <210> 497 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 497 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Xaa 1 5 10 <210> 498 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 498 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Xaa 1 5 10 <210> 499 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 499 Leu Thr Phe Ala Ala Phe Trp Ala Gln Leu Ala Ser 1 5 10 <210> 500 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 500 Leu Thr Phe Ala Ala Trp Trp Ala Gln Leu Ala Ser 1 5 10 <210> 501 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 501 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ile Ala Ser 1 5 10 <210> 502 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 502 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 503 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 503 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser Ala 1 5 10 <210> 504 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 504 Leu Thr Phe Ala Ala Trp Trp Ala Gln Leu Ala Ala 1 5 10 <210> 505 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 505 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ile Ala Ala 1 5 10 <210> 506 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 506 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 507 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 507 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala Ala 1 5 10 <210> 508 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 508 Leu Thr Phe Ala His Trp Trp Ala Gln Leu Ala Ser 1 5 10 <210> 509 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 509 Leu Thr Phe Ala His Arg Trp Ala Gln Leu Ala Ser 1 5 10 <210> 510 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 510 Leu Thr Phe Ala His Lys Trp Ala Gln Leu Ala Ser 1 5 10 <210> 511 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 511 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Trp 1 5 10 <210> 512 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Abu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 512 Phe Ala Ala Tyr Trp Xaa Ala Leu Ala Ala 1 5 10 <210> 513 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (3)..(3) <223> Abu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 513 Phe Ala Xaa Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 514 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Norleucine <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 514 Leu Pro Arg Phe Ala Asn Tyr Trp Arg Leu Leu Ala Gln Asn 1 5 10 <210> 515 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 515 Thr Ser Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 516 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 516 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 517 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 517 His Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 518 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 518 Leu His Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 519 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 519 Leu Thr Phe Ala His His Trp Ala Gln Leu Ala Ser 1 5 10 <210> 520 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 520 Leu Thr Phe Ala His Tyr Trp His Gln Leu Ala Ser 1 5 10 <210> 521 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 521 Leu Thr Phe Ala His Tyr Trp Ala His Leu Ala Ser 1 5 10 <210> 522 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 522 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala His 1 5 10 <210> 523 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHPr <220> <223> Crosslinked Sequence <400> 523 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 524 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHsBu <220> <223> Crosslinked Sequence <400> 524 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 525 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHiBu <220> <223> Crosslinked Sequence <400> 525 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 526 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHBn <220> <223> Crosslinked Sequence <400> 526 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 527 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHPe <220> <223> Crosslinked Sequence <400> 527 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 528 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHChx <220> <223> Crosslinked Sequence <400> 528 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 529 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 529 Glu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 530 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 530 Ser Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 531 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 531 Leu Glu Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 532 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 532 Leu Ser Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 533 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 533 Leu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 534 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 534 Leu Thr Phe Ala Ser Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 535 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 535 Leu Thr Phe Ala Ala Tyr Trp Glu Gln Leu Ala Ser 1 5 10 <210> 536 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 536 Leu Thr Phe Ala Ala Tyr Trp Ala Glu Leu Ala Ser 1 5 10 <210> 537 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 537 Leu Thr Phe Ala Ala Tyr Trp Ala Ser Leu Ala Ser 1 5 10 <210> 538 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 538 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Glu 1 5 10 <210> 539 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2CN <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 539 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 540 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 540 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 541 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 3,3-diphenyl-Ala <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 541 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 542 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Tert-butyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 542 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 543 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 543 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 544 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl6br-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 544 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 545 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl6f-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 545 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 546 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl4m-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 546 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 547 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl5cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 547 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 548 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl7m-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 548 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 549 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-methyl R8-octenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-methyl S5-pentenyl-alanine olefin amino acid connected by an all-carbon crosslinker that is fully saturated <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 549 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 550 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 550 Leu Thr Phe Ala His Xaa Trp Ala Gln Leu Ala Ser 1 5 10 <210> 551 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (6)..(6) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 551 Leu Thr Phe Glu His Xaa Trp Ala Gln Leu Thr Ser 1 5 10 <210> 552 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 552 Leu Thr Ala Ala His Xaa Trp Ala Gln Leu Ala Ser 1 5 10 <210> 553 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 553 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Xaa Ala 1 5 10 <210> 554 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 554 Phe Ala Ala Tyr Trp Glu Ala Ile Ala Ala Ala 1 5 10 <210> 555 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 555 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 556 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Alpha-Me-Leu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 556 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala Ala 1 5 10 <210> 557 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 557 Phe Ala Ala Tyr Trp Gln Ala Leu Ala Ala Ala 1 5 10 <210> 558 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 558 Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ala Ala 1 5 10 <210> 559 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Abu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 559 Phe Ala Ala Tyr Trp Xaa Ala Leu Ala Ala Ala 1 5 10 <210> 560 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 560 Phe Ala Ala Tyr Trp Leu Ala Leu Ala Ala Ala 1 5 10 <210> 561 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (3)..(3) <223> Abu <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 561 Phe Ala Xaa Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 562 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (3)..(3) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 562 Phe Ala Leu Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 563 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 563 Phe Ala Phe Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 564 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 564 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 565 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Adamantyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 565 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 566 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Indanyl-Gly <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 566 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 567 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 567 Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala Ala 1 5 10 <210> 568 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 568 Leu Thr Phe Ala Ala Leu Trp Ala Gln Leu Ala Gln 1 5 10 <210> 569 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 569 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 570 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 570 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Ala Ala 1 5 10 <210> 571 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 571 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Xaa 1 5 10 <210> 572 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Norleucine <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 572 Phe Ala Ala Tyr Trp Glu Leu Leu Ala Xaa 1 5 10 <210> 573 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 573 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Xaa 1 5 10 <210> 574 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Abu <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 574 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Xaa Ala 1 5 10 <210> 575 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> Aminocyclopropane carboxylic acid residue <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Norleucine <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 575 Phe Ala Ala Tyr Trp Glu Xaa Leu Ala Leu Ala 1 5 10 <210> 576 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term H <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 576 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 577 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term mdPEG3 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 577 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 578 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term mdPEG7 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 578 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 579 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (4)..(4) <223> Phosphoromethylphenylalanine-Et <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 579 Phe Ala Ala Phe Trp Glu Ala Leu Ala Ala 1 5 10 <210> 580 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 580 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 581 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 581 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 582 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 582 Leu Thr Phe Ala His Tyr Trp Trp Gln Leu Ala Ser 1 5 10 <210> 583 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 583 Leu Thr Phe Ala Ala Tyr Trp Trp Gln Leu Ala Ser 1 5 10 <210> 584 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 584 Leu Thr Phe Ala Ala Tyr Trp Trp Gln Leu Ala Ser 1 5 10 <210> 585 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 585 Leu Thr Phe Ala His Tyr Trp Trp Gln Leu Ala Ala 1 5 10 <210> 586 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 586 Leu Thr Phe Ala Ala Tyr Trp Trp Gln Leu Ala Ala 1 5 10 <210> 587 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 587 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 588 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-2F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 588 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 589 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 589 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 590 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-34F2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 590 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 591 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-35F2 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 591 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 592 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Phe-3Cl <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 592 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 593 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Phe-3Cl <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 593 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 594 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 594 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala Ala 1 5 10 <210> 595 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 595 Phe Ala Ala Tyr Trp Glu Ala Leu Ala 1 5 <210> 596 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Two pentenyl-alanine olefin side chains where one side chain is not crosslinked <220> <223> C-term NH2 <220> <223> May or may not be a Crosslinked Sequence <400> 596 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 597 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHsBu <220> <223> Crosslinked Sequence <400> 597 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 598 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 598 Ser Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 599 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 599 Leu Thr Phe Ala Ala Tyr Trp Ala Glu Leu Ala Ser 1 5 10 <210> 600 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 600 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Glu 1 5 10 <210> 601 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term mdPEG3 <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 601 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 602 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 602 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 603 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 603 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 604 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 604 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 605 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 605 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 606 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 606 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 607 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 607 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 608 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 608 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 609 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 609 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 610 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 610 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 611 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 611 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 612 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 612 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 613 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 613 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 614 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 614 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 615 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 615 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 616 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 616 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 617 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 617 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 618 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 618 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 619 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Phe <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 619 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 620 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Phe-3CF3 <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 620 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 621 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Phe-3Me <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 621 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 622 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 622 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 623 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Biphenylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 623 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 624 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 624 Leu Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 625 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHAm <220> <223> Crosslinked Sequence <400> 625 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 626 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHiAm <220> <223> Crosslinked Sequence <400> 626 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 627 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnPr3Ph <220> <223> Crosslinked Sequence <400> 627 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 628 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnBu3,3Me <220> <223> Crosslinked Sequence <400> 628 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 629 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnPr <220> <223> Crosslinked Sequence <400> 629 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 630 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnEt2Ch <220> <223> Crosslinked Sequence <400> 630 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 631 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHHex <220> <223> Crosslinked Sequence <400> 631 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 632 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHmdPeg2 <220> <223> Crosslinked Sequence <400> 632 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 633 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHmdPeg2 <220> <223> Crosslinked Sequence <400> 633 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 634 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHmdPeg4 <220> <223> Crosslinked Sequence <400> 634 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 635 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl4m-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 635 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 636 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> dl5cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 636 Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 637 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Homo-Phe <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 637 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 638 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 638 Leu Thr Ala Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 639 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 639 Leu Thr Ala Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 640 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 640 Leu Thr Phe Ala Ala Tyr Trp Val Gln Leu Ala Ser 1 5 10 <210> 641 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 641 Leu Thr Phe Ala His Tyr Trp Ala Ala Leu Ala Ala 1 5 10 <210> 642 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 642 Leu Thr Phe Ala Val Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 643 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 643 Leu Thr Phe Ala Ile Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 644 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 644 Phe Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 645 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 645 Ile Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 646 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 646 Ala Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 647 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 647 Ile Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 648 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 648 Phe Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 649 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 649 Trp Thr Phe Ala Val Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 650 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 650 Trp Thr Phe Ala Trp Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 651 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 651 Val Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 652 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 652 Trp Thr Phe Ala Phe Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 653 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 653 Phe Thr Phe Ala Ile Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 654 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 654 Trp Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 655 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 655 Phe Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 656 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 656 Tyr Thr Phe Ala Phe Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 657 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 6cl-Trp <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 657 Leu Thr Phe Ala Ala Tyr Trp Glu Ala Leu Ala Ala 1 5 10 <210> 658 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 6cl-Trp <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 658 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 659 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 659 Phe Ala Ala Tyr Trp Ser Ala Leu Ala Ala 1 5 10 <210> 660 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 660 Phe Ala Ala Tyr Trp Gln Ala Leu Ala Ala Ala 1 5 10 <210> 661 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 661 Leu His Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 662 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 662 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 663 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 663 Leu Thr Phe Ala Ala His Trp Ala Gln Leu Ala Ser 1 5 10 <210> 664 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 664 Leu Thr Phe Ala Ala Tyr Trp Ala His Leu Ala Ser 1 5 10 <210> 665 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 665 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala His 1 5 10 <210> 666 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term H <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 666 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 667 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 667 His His Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 668 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Ala <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 668 Ala Xaa Trp Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 669 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 669 Xaa Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 670 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 670 Xaa Ala Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 671 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Phe <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 671 Phe Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 672 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 672 Xaa Trp Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 673 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 673 Trp Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 674 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 674 Trp Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 675 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> D-Ala <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 675 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ala 1 5 10 <210> 676 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 676 Leu Thr Phe Ala Glu Tyr Trp Ala Arg Leu Ala Ala 1 5 10 <210> 677 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 677 Leu Thr Phe Ala Glu Tyr Trp Ala His Leu Ala Ala 1 5 10 <210> 678 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Ala <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 678 Ala Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 679 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 679 Xaa Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 680 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me-Phe <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 680 Phe Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 681 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me-Trp <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 681 Trp Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 682 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> NmLeu <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 682 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 683 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> NmThr <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 683 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 684 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Sarcosine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 684 Leu Xaa Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 685 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 685 Leu Gly Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 686 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> NmPhe <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 686 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 687 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 687 Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 688 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 688 Glu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 689 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 689 Leu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 690 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 690 Leu Thr Ala Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 691 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 691 Leu Thr Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ser 1 5 10 <210> 692 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 692 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 693 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 693 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 694 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 694 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 695 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Phe-3CF3 <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 695 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 696 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 696 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 697 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Biphenylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 697 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Phe Ala Ser 1 5 10 <210> 698 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 698 Leu Thr Ala Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 699 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 699 Leu Thr Phe Ala Ala Tyr Trp Val Gln Leu Ala Ser 1 5 10 <210> 700 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 700 Leu Thr Phe Ala Ala Trp Trp Ala Gln Leu Ala Ser 1 5 10 <210> 701 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 701 Phe Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 702 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 702 Ile Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 703 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 703 Ile Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 704 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 704 Phe Thr Phe Ala Phe Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 705 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 705 Val Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 706 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 706 Leu Thr Phe Ala Ala His Trp Ala Gln Leu Ala Ser 1 5 10 <210> 707 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 707 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala His 1 5 10 <210> 708 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 708 Leu Thr Phe Ala Ala Tyr Trp Ala His Leu Ala Ser 1 5 10 <210> 709 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Ala <220> <221> MOD_RES <222> (2)..(2) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 709 Ala Xaa Trp Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 710 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 710 Xaa Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 711 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 711 Xaa Ala Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 712 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Phe <220> <221> MOD_RES <222> (5)..(5) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 712 Phe Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 713 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (6)..(6) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (13)..(13) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 713 Xaa Trp Trp Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 714 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 714 Trp Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 715 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> Norleucine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 715 Trp Thr Phe Ala Leu Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 716 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (12)..(12) <223> D-Ala <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 716 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ala 1 5 10 <210> 717 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 717 Leu Thr Phe Ala Glu Tyr Trp Ala Arg Leu Ala Ala 1 5 10 <210> 718 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 718 Leu Thr Phe Ala Glu Tyr Trp Ala His Leu Ala Ala 1 5 10 <210> 719 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> D-Ala <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 719 Ala Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 720 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> 2-aminoisobutyric acid <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 720 Xaa Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 721 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me-Phe <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 721 Phe Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 722 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> Alpha-Me-Trp <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 722 Trp Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 723 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> NmLeu <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 723 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 724 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> NmThr <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 724 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 725 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Sarcosine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 725 Leu Xaa Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 726 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 726 Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 727 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 727 Glu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 728 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 728 Leu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 729 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 729 Trp Thr Phe Ala Val Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 730 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 730 Tyr Thr Phe Ala Phe Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 731 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (2)..(2) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> 6cl-Trp <220> <221> MOD_RES <222> (9)..(9) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 731 Phe Ala Ala Tyr Trp Ser Ala Leu Ala Ala 1 5 10 <210> 732 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 732 Glu Thr Phe Ala Glu Tyr Trp Val Gln Leu Ala Ser 1 5 10 <210> 733 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 733 Glu Thr Phe Ala Glu His Trp Ala Gln Leu Ala Ala 1 5 10 <210> 734 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 734 Ile Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 735 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 735 Ile Thr Phe Ala Glu His Trp Val Gln Leu Ala Ala 1 5 10 <210> 736 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 736 Ile Thr Phe Ala Glu His Trp Ala Gln Leu Ala Ser 1 5 10 <210> 737 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 737 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 738 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 738 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 739 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylalanine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 739 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 740 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 740 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 741 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-3Cl <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 741 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 742 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (7)..(7) <223> 6cl-Trp <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 742 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 743 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 743 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 744 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclobutyl-Ala <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 744 Leu Thr Phe Ala His Tyr Trp Ala Gln Ala Ala Ser 1 5 10 <210> 745 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Homo-Leu <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 745 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 746 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> Phe-4F <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (10)..(10) <223> Cyclohexylglycine <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 746 Leu Thr Phe Ala His Tyr Trp Ala Gln Gly Ala Ser 1 5 10 <210> 747 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 747 Glu Thr Phe Ala Glu Tyr Trp Val Ala Leu Ala Ser 1 5 10 <210> 748 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 748 Glu Thr Phe Ala Glu His Trp Ala Ala Leu Ala Ala 1 5 10 <210> 749 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 749 Ile Thr Phe Ala Glu Tyr Trp Ala Ala Leu Ala Ser 1 5 10 <210> 750 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 750 Ile Thr Phe Ala Glu His Trp Val Ala Leu Ala Ala 1 5 10 <210> 751 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 751 Ile Thr Phe Ala Glu His Trp Ala Ala Leu Ala Ser 1 5 10 <210> 752 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Pam <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 752 Leu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 753 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Pam <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 753 Glu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 754 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 754 Leu Thr Phe Ala Ala Tyr Trp Leu Gln Leu Ala Ser 1 5 10 <210> 755 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 755 Leu Thr Phe Ala Glu Tyr Trp Leu Gln Leu Ala Ser 1 5 10 <210> 756 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 756 Leu Thr Phe Ala Glu His Trp Leu Gln Leu Ala Ser 1 5 10 <210> 757 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 757 Leu Thr Phe Ala Val Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 758 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 758 Leu Thr Phe Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 759 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 759 Glu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 760 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 760 Leu Thr Phe Ala Glu Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 761 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHAm <220> <223> Crosslinked Sequence <400> 761 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 762 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHiAm <220> <223> Crosslinked Sequence <400> 762 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 763 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnPr3Ph <220> <223> Crosslinked Sequence <400> 763 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 764 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnBu3,3Me <220> <223> Crosslinked Sequence <400> 764 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 765 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnBu <220> <223> Crosslinked Sequence <400> 765 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 766 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnPr <220> <223> Crosslinked Sequence <400> 766 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 767 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHnEt2Ch <220> <223> Crosslinked Sequence <400> 767 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 768 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHHex <220> <223> Crosslinked Sequence <400> 768 Leu Thr Phe Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 769 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHmdPeg2 <220> <223> Crosslinked Sequence <400> 769 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 770 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NHmdPeg2 <220> <223> Crosslinked Sequence <400> 770 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 771 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 771 Leu Thr Phe Ala Ala Tyr Trp Ala Ala Leu Ala Ser 1 5 10 <210> 772 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 772 Leu Thr Phe Ala Ala Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 773 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 773 Leu Thr Phe Ala Xaa Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 774 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 774 Xaa Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 775 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term H <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 775 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ala 1 5 10 <210> 776 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 776 His His Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 777 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 777 Leu Thr Ala Ala Ala Tyr Trp Ser Gln Leu Ala Ser 1 5 10 <210> 778 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> (S)-2-amino-3-(7-methoxy-2-oxo-2H-chromene-3-yl) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 778 Leu Thr Phe Ala His Xaa Trp Ala Gln Leu Ala Ser 1 5 10 <210> 779 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (8)..(8) <223> (S)-2-amino-3-(7-(diethylamino)-2-oxo-2H-chromene-3-carboxamido) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 779 Leu Thr Phe Ala Ala Tyr Trp Xaa Gln Leu Ala Ser 1 5 10 <210> 780 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (5)..(5) <223> (S)-2-amino-3-(7-(diethylamino)-2-oxo-2H-chromene-3-carboxamido) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 780 Leu Thr Phe Ala Xaa Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 781 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (1)..(1) <223> (S)-2-amino-3-(7-(diethylamino)-2-oxo-2H-chromene-3-carboxamido) propanoic acid <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 781 Xaa Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 782 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (6)..(6) <223> (S)-2-amino-3-(7-(diethylamino)-2-oxo-2H-chromene-3-carboxamido) propanoic acid <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 782 Leu Thr Phe Ala Ala Xaa Trp Ala Gln Leu Ala Ser 1 5 10 <210> 783 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Dmaac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 783 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 784 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Hexac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 784 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 785 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Napac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 785 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 786 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Pam <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 786 Leu Thr Phe Ala Ala Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 787 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 787 Leu Thr Ala Ala His Tyr Ala Ala Gln Leu Ala Ser 1 5 10 <210> 788 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids that are not connected by any crosslinker <220> <223> C-term NH2 <400> 788 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 789 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 789 Leu Thr Ala Ala His Tyr Phe Ala Gln Leu Ala Ser 1 5 10 <210> 790 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 790 Leu Thr Ala Ala His Trp Ala Ala Gln Leu Ala Ser 1 5 10 <210> 791 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 791 Leu Thr Ala Ala His Tyr Ala Trp Gln Leu Ala Ser 1 5 10 <210> 792 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 2-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 792 Leu Thr Ala Ala His Tyr Ala Ala Gln Trp Ala Ser 1 5 10 <210> 793 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 793 Leu Thr Trp Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 794 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term Ac <220> <221> MOD_RES <222> (3)..(3) <223> 1-Naphthylalanine <220> <221> MOD_RES <222> (4)..(4) <223> Alpha-Me R8-octenyl-alanine olefin amino acids connected by an all-carbon i to i+7 crosslinker comprising one double bond <220> <221> MOD_RES <222> (11)..(11) <223> Alpha-Me S5-pentenyl-alanine olefin amino acids connected by an all-carbon i to i+4 crosslinker comprising one double bond <220> <223> C-term NH2 <220> <223> Crosslinked Sequence <400> 794 Leu Thr Ala Ala His Tyr Trp Ala Gln Leu Ala Ser 1 5 10 <210> 795 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic peptide <220> <223> N-term 5-FAM <220> <221> MOD_RES <222> (1)..(1) <223> Beta-Ala <220> <223> C-term NH2 <400> 795 Ala Leu Thr Phe Glu His Tyr Trp Ala Gln Leu Thr Ser 1 5 10 <210> 796 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic 6xHis tag <400> 796 His His His His His His 1 5

Claims (3)

  1. 서열 번호 689과 60% 이상 동일한 펩티도미메틱 거대고리(peptidomimetic macrocycle)로서, 펩티도미메틱 거대고리는 하기 화학식 I로 표시되고, a) 펩티도미메틱 거대고리는 α 나선을 포함하고, b) 펩티도미메틱 거대고리는 HDM2 및 HDMX에 결합하는 것인 펩티도미메틱 거대고리:
    [화학식 I]
    Figure pat00053

    상기 식에서,
    A, B, C, D 및 E는 각각 독립적으로 천연 또는 비천연 아미노산이고;
    [A]x-[B]y-[C]z는 트립토판 잔기 및 류신 잔기를 포함하고;
    [D]v는 페닐알라닌을 포함하고;
    R1 및 R2는 독립적으로 메틸이고;
    L은 화학식 -L1-L2-의 거대고리 형성 연결제(linker)이고;
    L1 및 L2는 독립적으로 C3-C6 알킬렌 또는 C3-C6 알케닐렌이고;
    R7 및 R8은 독립적으로 -H이고;
    v 및 w는 독립적으로 1 내지 1000의 정수이고;
    u는 1이고;
    x+y+z는 6이다.
  2. 제1항에 있어서, 피험체에서 암을 치료하는데 사용하기 위한 펩티도미메틱 거대고리.
  3. 제1항의 펩티도미메틱 거대고리를 포함하는, 암을 치료하기 위한 약학 조성물.
KR1020197016502A 2010-08-13 2011-08-13 펩티도미메틱 거대고리 KR102104762B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37370110P 2010-08-13 2010-08-13
US37363810P 2010-08-13 2010-08-13
US61/373,701 2010-08-13
US61/373,638 2010-08-13
US37416310P 2010-08-16 2010-08-16
US61/374,163 2010-08-16
PCT/US2011/047692 WO2012021876A2 (en) 2010-08-13 2011-08-13 Peptidomimetic macrocycles

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187014674A Division KR20180058866A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리

Publications (2)

Publication Number Publication Date
KR20190069603A true KR20190069603A (ko) 2019-06-19
KR102104762B1 KR102104762B1 (ko) 2020-04-24

Family

ID=45567961

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020177013069A KR20170058446A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리
KR1020137006479A KR20130099938A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리
KR1020187014674A KR20180058866A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리
KR1020197016502A KR102104762B1 (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리

Family Applications Before (3)

Application Number Title Priority Date Filing Date
KR1020177013069A KR20170058446A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리
KR1020137006479A KR20130099938A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리
KR1020187014674A KR20180058866A (ko) 2010-08-13 2011-08-13 펩티도미메틱 거대고리

Country Status (15)

Country Link
US (5) US8859723B2 (ko)
EP (1) EP2603600B1 (ko)
JP (3) JP2013535514A (ko)
KR (4) KR20170058446A (ko)
CN (2) CN103282510A (ko)
BR (1) BR112013003248A2 (ko)
CA (1) CA2807685C (ko)
DK (1) DK2603600T3 (ko)
ES (1) ES2711526T3 (ko)
MX (1) MX355543B (ko)
PL (1) PL2603600T3 (ko)
PT (1) PT2603600T (ko)
RU (2) RU2582678C2 (ko)
SI (1) SI2603600T1 (ko)
WO (3) WO2012021875A1 (ko)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105061577A (zh) 2006-12-14 2015-11-18 爱勒让治疗公司 双巯基大环化系统
US8889632B2 (en) 2007-01-31 2014-11-18 Dana-Farber Cancer Institute, Inc. Stabilized p53 peptides and uses thereof
CN109627287A (zh) 2007-02-23 2019-04-16 爱勒让治疗公司 三唑大环系统
CN101730708B (zh) 2007-03-28 2013-09-18 哈佛大学校长及研究员协会 缝合多肽
CN102482336B (zh) 2008-09-22 2015-05-06 爱勒让治疗公司 拟肽大环化合物
US9458202B2 (en) 2008-11-24 2016-10-04 Aileron Therapeutics, Inc. Peptidomimetic macrocycles with improved properties
JP2012515172A (ja) 2009-01-14 2012-07-05 エルロン・セラピューティクス・インコーポレイテッド ペプチド模倣大環状分子
CA2774973A1 (en) 2009-09-22 2011-03-31 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
BR112012008831A2 (pt) * 2009-10-14 2019-09-24 Aileron Therapeutics Inc macrociclos de peptideomiméticos aprimorados.
JP2013514968A (ja) * 2009-12-22 2013-05-02 武田薬品工業株式会社 徐放性製剤
FR2956115B1 (fr) 2010-02-05 2012-04-06 Isp Investments Inc Nouveaux peptides activateurs de la caspase-14 et compositions les comprenant
CA2807685C (en) 2010-08-13 2020-10-06 Aileron Therapeutics, Inc. P53 derived peptidomimetic macrocycle
US9096684B2 (en) 2011-10-18 2015-08-04 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
JP6450192B2 (ja) * 2012-02-15 2019-01-09 エイルロン セラピューティクス，インコーポレイテッド トリアゾール架橋した、およびチオエーテル架橋したペプチドミメティック大環状化合物
WO2013123266A1 (en) * 2012-02-15 2013-08-22 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
EP2904000A4 (en) * 2012-10-01 2016-04-20 Agency Science Tech & Res PEPTIDES AND METHODS OF TREATING CANCER
US9604919B2 (en) 2012-11-01 2017-03-28 Aileron Therapeutics, Inc. Disubstituted amino acids and methods of preparation and use thereof
WO2014138429A2 (en) 2013-03-06 2014-09-12 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and use thereof in regulating hif1alpha
US11168115B2 (en) 2013-06-20 2021-11-09 California Institute Of Technology Cyclic peptides as protein targeting agents
CN106604928A (zh) * 2014-04-02 2017-04-26 罗契斯特大学 用于α‑螺旋拟态的大环肽模拟物
WO2015157508A1 (en) * 2014-04-09 2015-10-15 Aileron Therapeutics, Inc. Peptidomimetic macrocycles with pth activity
AU2015320549A1 (en) * 2014-09-24 2017-04-13 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
SG11201702175YA (en) 2014-09-24 2017-04-27 Aileron Therapeutics Inc Peptidomimetic macrocycles and formulations thereof
BR112017019738A2 (pt) * 2015-03-20 2018-05-29 Aileron Therapeutics Inc macrociclos peptidomiméticos e usos dos mesmos
WO2017004548A1 (en) 2015-07-01 2017-01-05 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
US9765117B2 (en) 2015-08-24 2017-09-19 Romek Figa Peptides for treating cancer
WO2017040990A1 (en) * 2015-09-03 2017-03-09 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
EP3347372A4 (en) 2015-09-10 2019-09-04 Aileron Therapeutics, Inc. PEPTIDOMIMETIC MACROCYCLES AS MODULATORS OF MCL-1
JP7286317B2 (ja) 2015-10-27 2023-06-05 エフ． ホフマン－ラ ロシュ アーゲー Ａｃｉｎｅｔｏｂａｃｔｅｒ ｂａｕｍａｎｎｉｉに対するペプチド大環状分子
DK3430031T3 (da) * 2016-03-15 2022-01-17 Idp Discovery Pharma S L Peptider med anti-kræftaktivitet
EP3432904A4 (en) * 2016-03-21 2020-03-11 Aileron Therapeutics, Inc. COMPANION DIAGNOSTIC TOOL FOR PEPTIDOMIMETIC MACROCYCLES
EP3388444A1 (en) 2017-04-10 2018-10-17 F. Hoffmann-La Roche AG Anti-bacterial peptide macrocycles and use thereof
WO2019020649A1 (en) * 2017-07-25 2019-01-31 Idp Discovery Pharma, S.L. ANTICANCER PEPTIDES
WO2019090015A1 (en) * 2017-11-03 2019-05-09 Yale University Peptidic materials that traffic efficiently to the cell cytosol and nucleus
WO2019133179A2 (en) * 2017-12-01 2019-07-04 Massachusetts Institute Of Technology Solution-phase affinity selection of inhibitors from combinatorial peptide libraries
CA3078682A1 (en) 2017-12-15 2019-06-20 Dana-Farber Cancer Institute, Inc. Stabilized peptide-mediated targeted protein degradation
US11505573B2 (en) 2018-03-28 2022-11-22 Hoffmann-La Roche Inc. Peptide macrocycles against Acinetobacter baumannii
US11819532B2 (en) 2018-04-23 2023-11-21 Hoffmann-La Roche Inc. Peptide macrocycles against Acinetobacter baumannii
US11091522B2 (en) 2018-07-23 2021-08-17 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
US11919972B2 (en) 2018-11-02 2024-03-05 Regeneron Pharmaceuticals, Inc. Peptide libraries with non-canonical amino acids
US20200289609A1 (en) * 2019-03-15 2020-09-17 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
CN110721314B (zh) * 2019-10-11 2022-11-29 江苏恒泰生物科技有限公司 一种抗肿瘤纳米药物及其制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137974A2 (en) * 2005-06-13 2006-12-28 Tranzyme Pharma, Inc. Macrocyclic ghrelin receptor antagonists and inverse agonists and methods of using the same
WO2009126292A2 (en) * 2008-04-08 2009-10-15 Aileron Therapeutics, Inc. Biologically active peptidomimetic macrocycles

Family Cites Families (573)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1030010A (en) 1907-05-27 1912-06-18 Vermont Farm Machine Company Cow-milking machine.
US1021347A (en) 1908-03-07 1912-03-26 Hans Windhoff Hoisting-gear.
US1025306A (en) 1909-06-24 1912-05-07 Randolph R Rawle Apparatus for aerial navigation.
US1005974A (en) 1910-03-30 1911-10-17 Samuel N House Tenoning-machine.
US1024649A (en) 1910-05-16 1912-04-30 Peter Peel Animal-releasing means.
US1020243A (en) 1910-06-17 1912-03-12 Gustav Adolf Wlost Resonating device.
US1002361A (en) 1910-10-13 1911-09-05 Daniel N Baxter Boiler-cleaner.
US1002242A (en) 1910-12-27 1911-09-05 Otto P W Ehrhardt Cabbage-corer.
US1003004A (en) 1911-01-16 1911-09-12 Gustave J Martel Repair-patch for pneumatic tires.
US1032811A (en) 1911-02-01 1912-07-16 James O Brookbank Burner.
US1030135A (en) 1911-05-13 1912-06-18 Belisario Silfa Spark-plug.
US1022738A (en) 1911-10-24 1912-04-09 Julius W Horvath Spring-bed.
US1030869A (en) 1911-11-06 1912-07-02 Vittorio Buffulini Wreck-raising appliance.
US1048711A (en) 1912-02-23 1912-12-31 John Uri Lloyd Alkaloidal substance.
US1046497A (en) 1912-03-15 1912-12-10 Adam Sawicki Nail holding and delivering apparatus.
US1047112A (en) 1912-07-17 1912-12-10 Ole P Noisom Electric-circuit controller.
US4000259A (en) 1975-06-16 1976-12-28 American Home Products Corporation Cyclic dodecapeptide analogs of somatostatin and intermediates
US4438270A (en) 1977-07-11 1984-03-20 Merrell Toraude Et Compagnie α-Halomethyl derivatives of α-amino acids
US4191754A (en) 1979-02-28 1980-03-04 Merck & Co., Inc. Bicyclic somatostatin analogs
US4270537A (en) 1979-11-19 1981-06-02 Romaine Richard A Automatic hypodermic syringe
AU550730B2 (en) 1982-03-09 1986-04-10 Commonwealth Of Australia, The Automated metal detection
US4728726A (en) 1982-10-04 1988-03-01 The Salk Institute For Biological Studies GRF analogs IIIb
US4518586A (en) 1983-01-13 1985-05-21 The Salk Institute For Biological Studies GRF Analogs III
US5416073A (en) 1983-08-10 1995-05-16 The Adminstrators Of The Tulane Educational Fund Growth hormone-releasing peptides and method of treating animals, therewith
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5036045A (en) 1985-09-12 1991-07-30 The University Of Virginia Alumni Patents Foundation Method for increasing growth hormone secretion
US4730006A (en) 1986-01-27 1988-03-08 Merrell Dow Pharmaceuticals Inc. Derivatives of 2,6-diamino-3-haloheptanedioic acid
US4880778A (en) 1986-05-12 1989-11-14 Eastman Kodak Company Combinations having synergistic growth hormone releasing activity and methods for use thereof
HU906340D0 (en) 1986-10-13 1991-04-29 Sandoz Ag Synthesis in solid phase for producing peptonic alcohols
CA1283827C (en) 1986-12-18 1991-05-07 Giorgio Cirelli Appliance for injection of liquid formulations
GB8704027D0 (en) 1987-02-20 1987-03-25 Owen Mumford Ltd Syringe needle combination
US5112808A (en) 1987-05-11 1992-05-12 American Cyanamid Company Alkylated hormone-releasing peptides and method of treatig mammals therewith
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
US4940460A (en) 1987-06-19 1990-07-10 Bioject, Inc. Patient-fillable and non-invasive hypodermic injection device assembly
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
US5877277A (en) 1987-09-24 1999-03-02 Biomeasure, Inc. Octapeptide bombesin analogs
US5453418A (en) 1988-03-07 1995-09-26 Eli Lilly And Company Ractopamine and growth hormone combinations
US5339163A (en) 1988-03-16 1994-08-16 Canon Kabushiki Kaisha Automatic exposure control device using plural image plane detection areas
WO1989009233A1 (en) 1988-03-24 1989-10-05 Terrapin Technologies, Inc. Molecular sticks for controlling protein conformation
US5094951A (en) 1988-06-21 1992-03-10 Chiron Corporation Production of glucose oxidase in recombinant systems
US5043322A (en) 1988-07-22 1991-08-27 The Salk Institute For Biological Studies Cyclic GRF analogs
FR2638359A1 (fr) 1988-11-03 1990-05-04 Tino Dalto Guide de seringue avec reglage de la profondeur de penetration de l'aiguille dans la peau
US5384309A (en) 1989-07-17 1995-01-24 Genentech, Inc. Cyclized peptides and their use as platelet aggregation inhibitors
US5120859A (en) 1989-09-22 1992-06-09 Genentech, Inc. Chimeric amino acid analogues
US5712418A (en) 1989-10-23 1998-01-27 Research Corporation Technologies, Inc. Synthesis and use of amino acid fluorides as peptide coupling reagents
US5650133A (en) 1990-01-19 1997-07-22 Nycomed Salutar Macrocyclic polyaza dichelates linked through ring nitrogens via an amide or ester functionality
US5169932A (en) 1989-10-30 1992-12-08 The Salk Institute For Biological Studies Gnrh analogs
US5580957A (en) 1989-10-30 1996-12-03 The Salk Institute For Biological Studies GnRH analogs
US5352796A (en) 1989-10-30 1994-10-04 The Salk Institute For Biological Studies Amino acids useful in making GnRH analogs
US5296468A (en) 1989-10-30 1994-03-22 The Salk Institute For Biological Studies GnRH analogs
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
US5245009A (en) 1990-03-23 1993-09-14 The Salk Institute For Biological Studies CRF antagonists
CA2047042A1 (en) 1990-07-19 1992-01-20 John Hannah Cyclic hiv principal neutralizing determinant peptides
US6331318B1 (en) 1994-09-30 2001-12-18 Emisphere Technologies Inc. Carbon-substituted diketopiperazine delivery systems
US5629020A (en) 1994-04-22 1997-05-13 Emisphere Technologies, Inc. Modified amino acids for drug delivery
US5714167A (en) 1992-06-15 1998-02-03 Emisphere Technologies, Inc. Active agent transport systems
US5190521A (en) 1990-08-22 1993-03-02 Tecnol Medical Products, Inc. Apparatus and method for raising a skin wheal and anesthetizing skin
JPH06501950A (ja) 1990-10-11 1994-03-03 ベーリンガー　インゲルハイム　コマンディトゲゼルシャフト 環状ペプチド、その調製法およびその薬理組成物としての使用
DE69118826T2 (de) 1990-11-27 1996-11-14 Fuji Photo Film Co Ltd Propenamidderivate, deren Polymere, Copolymere und deren Verwendung
US5124454A (en) 1990-11-30 1992-06-23 Minnesota Mining And Manufacturing Company Polycyclic diamines and method of preparation
US5527288A (en) 1990-12-13 1996-06-18 Elan Medical Technologies Limited Intradermal drug delivery device and method for intradermal delivery of drugs
WO1992013878A2 (en) 1991-02-07 1992-08-20 Board Of Trustees Of The University Of Illinois Conformationally restricted mimetics of beta turns and beta bulges and peptides containing the same
US5747469A (en) * 1991-03-06 1998-05-05 Board Of Regents, The University Of Texas System Methods and compositions comprising DNA damaging agents and p53
US5744450A (en) 1991-03-14 1998-04-28 The Salk Institute For Biological Studies GnRH analogs
EP0525838A3 (en) 1991-04-09 1993-02-10 F. Hoffmann-La Roche Ag Growth hormone releasing factor analogs
US5262519A (en) 1991-05-15 1993-11-16 The Salk Institute For Biological Studies GRF analogs XI
US5364851A (en) 1991-06-14 1994-11-15 International Synthecon, Llc Conformationally restricted biologically active peptides, methods for their production and uses thereof
CA2072249C (en) 1991-06-28 2003-06-17 Saiko Hosokawa Human monoclonal antibody specifically binding to surface antigen of cancer cell membrane
GB9114949D0 (en) 1991-07-11 1991-08-28 Smithkline Beecham Plc Novel compounds
ATE155486T1 (de) 1991-08-13 1997-08-15 Takeda Chemical Industries Ltd Zyklische peptide und ihre verwendung
GB9118204D0 (en) 1991-08-23 1991-10-09 Weston Terence E Needle-less injector
US7517644B1 (en) 1991-08-23 2009-04-14 Larry J. Smith Method and compositions for cellular reprogramming
SE9102652D0 (sv) 1991-09-13 1991-09-13 Kabi Pharmacia Ab Injection needle arrangement
IL103252A (en) 1991-09-30 1997-03-18 Du Pont Merck Pharma CYCLIC COMPOUNDS USEFUL AS INHIBITORS OF PLATELET GLYCOPROTEIN IIb/IIIa AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM
ES2133295T3 (es) 1991-11-19 1999-09-16 Takeda Chemical Industries Ltd Peptidos ciclicos y su uso.
US5328483A (en) 1992-02-27 1994-07-12 Jacoby Richard M Intradermal injection device with medication and needle guard
AU691645B2 (en) 1992-04-03 1998-05-21 California Institute Of Technology High activity ruthenium or osmium metal carbene complexes for olefin metathesis reactions and synthesis thereof
US5411860A (en) 1992-04-07 1995-05-02 The Johns Hopkins University Amplification of human MDM2 gene in human tumors
WO1993024525A1 (en) 1992-05-26 1993-12-09 Rijksuniversiteit Leiden PEPTIDES OF HUMAN p53 PROTEIN FOR USE IN HUMAN T CELL RESPONSE INDUCING COMPOSITIONS, AND HUMAN p53 PROTEIN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES
US5323907A (en) 1992-06-23 1994-06-28 Multi-Comp, Inc. Child resistant package assembly for dispensing pharmaceutical medications
JPH07509128A (ja) 1992-07-13 1995-10-12 バイオネブラスカ・インコーポレーテッド 組換型ポリペプチドの修飾方法
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US5569189A (en) 1992-09-28 1996-10-29 Equidyne Systems, Inc. hypodermic jet injector
US5334144A (en) 1992-10-30 1994-08-02 Becton, Dickinson And Company Single use disposable needleless injector
US5371070A (en) 1992-11-09 1994-12-06 The Salk Institute For Biological Studies Bicyclic GnRH antagonists and a method for regulating the secretion of gonadotropins
JPH08507070A (ja) 1993-02-22 1996-07-30 アルザ・コーポレーション 活性物質の経口投与のための組成物
AU6415894A (en) 1993-03-29 1994-10-24 Du Pont Merck Pharmaceutical Company, The Cyclic compounds useful as inhibitors of platelet glycoprotein iib/iiia
US5643957A (en) 1993-04-22 1997-07-01 Emisphere Technologies, Inc. Compounds and compositions for delivering active agents
CA2161108A1 (en) 1993-04-23 1994-11-10 Herbert J. Evans Polypeptides that include conformation-constraining groups which flank a protein-protein interaction site
US5446128A (en) 1993-06-18 1995-08-29 The Board Of Trustees Of The University Of Illinois Alpha-helix mimetics and methods relating thereto
PL180612B1 (pl) 1993-08-09 2001-03-30 Biomeasure Pochodna peptydu PL PL PL PL PL PL PL PL PL
US5622852A (en) 1994-10-31 1997-04-22 Washington University Bcl-x/Bcl-2 associated cell death regulator
US5536814A (en) 1993-09-27 1996-07-16 La Jolla Cancer Research Foundation Integrin-binding peptides
AU1211895A (en) 1993-11-22 1995-06-13 Onyx Pharmaceuticals P53-binding polypeptides and polynucleotides encoding same
US6287787B1 (en) 1993-11-24 2001-09-11 Torrey Pines Institute For Molecular Studies Dimeric oligopeptide mixture sets
WO1995022546A1 (en) 1994-02-18 1995-08-24 Cell Therapeutics, Inc. Intracellular signalling mediators
WO1995024176A1 (en) 1994-03-07 1995-09-14 Bioject, Inc. Ampule filling device
US5466220A (en) 1994-03-08 1995-11-14 Bioject, Inc. Drug vial mixing and transfer device
US5506207A (en) 1994-03-18 1996-04-09 The Salk Institute For Biological Studies GNRH antagonists XIII
US5824483A (en) 1994-05-18 1998-10-20 Pence Inc. Conformationally-restricted combinatiorial library composition and method
JP3166482B2 (ja) 1994-06-07 2001-05-14 日産自動車株式会社 反射干渉作用を有する発色構造体
US6407059B1 (en) 1994-06-08 2002-06-18 Peptor Limited Conformationally constrained backbone cyclized peptide analogs
IL109943A (en) 1994-06-08 2006-08-01 Develogen Israel Ltd Conformationally constrained backbone cyclized peptide analogs
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
US7553929B2 (en) 1994-06-13 2009-06-30 Vanderbilt University Cell permeable peptides for inhibition of inflammatory reactions and methods of use
US5770377A (en) 1994-07-20 1998-06-23 University Of Dundee Interruption of binding of MDM2 and P53 protein and therapeutic application thereof
US5702908A (en) 1994-07-20 1997-12-30 University Of Dundee Interruption of binding of MDM2 and p53 protein and therapeutic application thereof
EP0782709B1 (en) 1994-09-19 2009-11-18 Ricardo J. Moro Detection of cancer
CA2158782C (en) 1994-09-23 2010-01-12 Pierrette Gaudreau Marker for growth hormone-releasing factor receptors
US5681928A (en) 1994-12-16 1997-10-28 The Salk Institute For Biological Studies Betides and methods for screening peptides using same
CA2209183A1 (en) 1994-12-29 1996-07-11 Joel L. Pomerantz Chimeric dna-binding proteins
US5599302A (en) 1995-01-09 1997-02-04 Medi-Ject Corporation Medical injection system and method, gas spring thereof and launching device using gas spring
US5792747A (en) 1995-01-24 1998-08-11 The Administrators Of The Tulane Educational Fund Highly potent agonists of growth hormone releasing hormone
US6169073B1 (en) 1995-02-16 2001-01-02 Bayer Corporation Peptides and peptidomimetics with structural similarity to human p53 that activate p53 function
EP0729972A1 (de) 1995-02-28 1996-09-04 F. Hoffmann-La Roche Ag Tetrahydronaphthalin-Peptidderivate
US6514942B1 (en) 1995-03-14 2003-02-04 The Board Of Regents, The University Of Texas System Methods and compositions for stimulating T-lymphocytes
US5675001A (en) 1995-03-14 1997-10-07 Hoffman/Barrett, L.L.C. Heteroatom-functionalized porphyrazines and multimetallic complexes and polymers derived therefrom
US5700775A (en) 1995-03-24 1997-12-23 Gutniak; Mark K. Method and treatment composition for decreasing patient time in catabolic state after traumatic injury
BR9604880A (pt) 1995-03-31 1998-05-19 Emisphere Tech Inc Composto composição forma de unidade de dosagem métodos para administração de um agente biologicamente ativo para preparar uma composição para administração de um agente ativo e para preparar um composto e composição farmacológica
US5650386A (en) 1995-03-31 1997-07-22 Emisphere Technologies, Inc. Compositions for oral delivery of active agents
US6090958A (en) 1995-03-31 2000-07-18 Emisphere Technologies, Inc. Compounds and compositions for delivering active agents
US5731408A (en) 1995-04-10 1998-03-24 Arizona Board Of Regents On Behalf Of The University Of Arizona Peptides having potent antagonist and agonist bioactivities at melanocortin receptors
US6054556A (en) 1995-04-10 2000-04-25 The Arizona Board Of Regents On Behalf Of The University Of Arizona Melanocortin receptor antagonists and agonists
WO1996032126A1 (en) 1995-04-14 1996-10-17 The Administrators Of The Tulane Educational Fund Analogs of growth hormone-releasing factor
US5672584A (en) 1995-04-25 1997-09-30 The University Of Kansas Cyclic prodrugs of peptides and peptide nucleic acids having improved metabolic stability and cell membrane permeability
US6184344B1 (en) 1995-05-04 2001-02-06 The Scripps Research Institute Synthesis of proteins by native chemical ligation
MX9708500A (es) 1995-05-04 1998-02-28 Scripps Research Inst Sintesis de proteinas mediante ligacion quimica nativa.
US6458764B1 (en) 1995-05-26 2002-10-01 Theratechnologies Inc. GRF analogs with increased biological potency
US6020311A (en) 1995-05-26 2000-02-01 Theratechnologies, Inc. GRF analogs with increased biological potency
WO1996037514A1 (en) 1995-05-26 1996-11-28 Theratechnologies Inc. Chimeric fatty body-pro-grf analogs with increased biological potency
US5730723A (en) 1995-10-10 1998-03-24 Visionary Medical Products Corporation, Inc. Gas pressured needle-less injection device and method
US5817789A (en) 1995-06-06 1998-10-06 Transkaryotic Therapies, Inc. Chimeric proteins for use in transport of a selected substance into cells
US6051554A (en) 1995-06-07 2000-04-18 Peptor Limited Conformationally constrained backbone cyclized somatostatin analogs
US6413994B1 (en) 1999-02-22 2002-07-02 The Salk Institute For Biological Studies Modulators of peroxisome proliferator activated receptor-gamma, and methods for the use thereof
US5811515A (en) 1995-06-12 1998-09-22 California Institute Of Technology Synthesis of conformationally restricted amino acids, peptides, and peptidomimetics by catalytic ring closing metathesis
FR2738151B1 (fr) 1995-09-04 1997-09-26 Rhone Poulenc Rorer Sa Antagonistes de l'activite oncogenique de la proteine mdm2, et leur utilisation dans le traitement des cancers
EP0766966A3 (en) 1995-09-08 2001-02-28 Eli Lilly And Company Method of treating insulin resistance
US5750499A (en) 1995-10-18 1998-05-12 The Salk Institute For Biological Studies Receptor-selective somatostatin analogs
GB9521544D0 (en) 1995-10-20 1995-12-20 Univ Dundee Activation of P53 protein and therapeutic applications thereof
US5840833A (en) 1995-10-27 1998-11-24 Molecumetics, Ltd Alpha-helix mimetics and methods relating thereto
US6123964A (en) 1995-10-27 2000-09-26 Merck & Co., Inc. Wet granulation formulation of a growth hormone secretagogue
ATE424412T1 (de) 1995-12-22 2009-03-15 Novo Nordisk As Verbindungen mit wachstumshormon-freisetzenden eigenschaften
US5807983A (en) 1995-12-28 1998-09-15 The Salk Institute For Biological Studies GNRH antagonist betides
US5893397A (en) 1996-01-12 1999-04-13 Bioject Inc. Medication vial/syringe liquid-transfer apparatus
EP0880357A4 (en) 1996-01-17 1999-05-12 California Inst Of Techn SYNTHESIS OF CONFORMATORICALLY RESTRICTED AMINO ACIDS, PEPTIDES AND PEPTIDOMIMETIC COMPOUNDS BY CATALYTIC RINGCLOSE METATHESIS
US5849954A (en) 1996-01-18 1998-12-15 Research Corporation Technologies, Inc. Method of peptide synthesis
US5849691A (en) 1996-02-20 1998-12-15 The United States Of America As Represented By The Department Of Health And Human Services Peptidomimetic inhibitors of cathepsin D and plasmepsins I and II
SI9720025A (sl) 1996-03-29 1999-08-31 Emishphere Technologies, Inc. Spojine in sestavki za prenos aktivne snovi
GB9607549D0 (en) 1996-04-11 1996-06-12 Weston Medical Ltd Spring-powered dispensing device
JP3792777B2 (ja) 1996-05-10 2006-07-05 株式会社カネカ １−アルコキシカルボニル−３−フェニルプロピル誘導体の製造方法
AU3220097A (en) 1996-05-22 1997-12-09 Arch Development Corporation Sleep quality improvement using a growth hormone secretagogue
US5817752A (en) 1996-06-06 1998-10-06 La Jolla Pharmaceutical Company Cyclic polypeptides comprising a thioether linkage and methods for their preparation
US5817627A (en) 1996-06-14 1998-10-06 Theratechnologies Inc. Long-acting galenical formulation for GRF peptides
EP0812856B1 (en) 1996-06-14 1999-09-29 Takeda Chemical Industries, Ltd. Method for removing N-terminal methionine
US5663316A (en) 1996-06-18 1997-09-02 Clontech Laboratories, Inc. BBC6 gene for regulation of cell death
US7083983B2 (en) 1996-07-05 2006-08-01 Cancer Research Campaign Technology Limited Inhibitors of the interaction between P53 and MDM2
NZ333609A (en) 1996-07-05 2000-08-25 Cancer Res Campaign Tech Peptide inhibitors of the interaction between P35 and MDM2 and the treatment of tumor cells
WO1998003473A1 (en) 1996-07-22 1998-01-29 Novo Nordisk A/S Compounds with growth hormone releasing properties
US5955593A (en) 1996-09-09 1999-09-21 Washington University BH3 interacting domain death agonist
US20020064546A1 (en) 1996-09-13 2002-05-30 J. Milton Harris Degradable poly(ethylene glycol) hydrogels with controlled half-life and precursors therefor
US5965703A (en) 1996-09-20 1999-10-12 Idun Pharmaceuticals Human bad polypeptides, encoding nucleic acids and methods of use
GB9619757D0 (en) 1996-09-21 1996-11-06 Knoll Ag Chemical process
US5856445A (en) 1996-10-18 1999-01-05 Washington University Serine substituted mutants of BCL-XL /BCL-2 associated cell death regulator
JP4024309B2 (ja) 1996-10-22 2007-12-19 第一三共株式会社 新規な感染症治療薬
US6271198B1 (en) 1996-11-06 2001-08-07 Genentech, Inc. Constrained helical peptides and methods of making same
AU734337B2 (en) 1996-11-21 2001-06-14 Promega Corporation Alkyl peptide amides and applications
CZ290347B6 (cs) 1997-02-07 2002-07-17 Ústav organické chemie a biochemie AV ČR Cyklopeptidy stimulující uvolňování růstového hormonu a způsob jejich přípravy
US6313088B1 (en) 1997-02-07 2001-11-06 Emisphere Technologies, Inc. 8-[(2-hydroxy-4-methoxy benzoyl) amino]-octanoic acid compositions for delivering active agents
US6060513A (en) 1997-02-07 2000-05-09 Emisphere Technologies, Inc. Compounds and compositions for delivering active agents
JP2001512475A (ja) 1997-02-20 2001-08-21 イエダ リサーチ アンド デベロップメント カンパニー リミテッド 抗病原性合成ペプチドおよびこれらを含む組成物
US6849428B1 (en) 1997-03-05 2005-02-01 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
US6635740B1 (en) 1997-03-27 2003-10-21 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Ligand/lytic peptide compositions and methods of use
AU6895598A (en) 1997-04-11 1998-11-11 Eli Lilly And Company Combinatorial libraries of peptidomimetic macrocycles and processes therefor
GB9708092D0 (en) 1997-04-22 1997-06-11 Univ Dundee Materials and methods relating to inhibiting the interaction of p53 and mdm2
US6329368B1 (en) 1997-05-09 2001-12-11 The Regents Of The University Of California Endocrine modulation with positive modulators of AMPA type glutamate receptors
US5993412A (en) 1997-05-19 1999-11-30 Bioject, Inc. Injection apparatus
US6127341A (en) 1997-06-20 2000-10-03 Novo Nordisk A/S Compounds with growth hormone releasing properties
US6248358B1 (en) 1998-08-25 2001-06-19 Columbia Laboratories, Inc. Bioadhesive progressive hydration tablets and methods of making and using the same
US7064193B1 (en) 1997-09-17 2006-06-20 The Walter And Eliza Hall Institute Of Medical Research Therapeutic molecules
US6420136B1 (en) 1997-09-26 2002-07-16 University Technologies International, Inc. Method of modulating p53 activity
US6165732A (en) 1997-10-14 2000-12-26 Washington University Method for identifying apoptosis modulating compounds
US6875594B2 (en) 1997-11-13 2005-04-05 The Rockefeller University Methods of ligating expressed proteins
WO1999029343A1 (en) * 1997-12-09 1999-06-17 Thomas Jefferson University Method of treating bladder cancer with wild type vaccinia virus
ATE255422T1 (de) 1998-01-07 2003-12-15 Debio Rech Pharma Sa Abbaubare, heterobifunktionelle polyethylenglykolacrylate, sowie damit herstellbare gele und konjugate
IT1298087B1 (it) 1998-01-08 1999-12-20 Fiderm S R L Dispositivo per il controllo della profondita' di penetrazione di un ago, in particolare applicabile ad una siringa per iniezioni
US6030997A (en) 1998-01-21 2000-02-29 Eilat; Eran Acid labile prodrugs
IL137388A (en) 1998-01-29 2005-05-17 Poly Med Inc Absorbable microparticles
DK1051194T3 (da) 1998-01-29 2003-09-01 Kinerton Ltd Fremgangsmåde til fremstilling af absorberbare mikropartikler
JP4394279B2 (ja) 1998-03-09 2010-01-06 ジーランド ファーマ アクティーゼルスカブ 酵素加水分解に対する傾向が減少した薬理学的に活性なペプチド複合体
AU767185B2 (en) 1998-03-23 2003-11-06 President And Fellows Of Harvard College Synthesis of compounds and libraries of compounds
AP1117A (en) 1998-04-15 2002-11-02 Aventis Pharma Inc Process for the preparation of resin-bound cyclic peptides.
US6190699B1 (en) 1998-05-08 2001-02-20 Nzl Corporation Method of incorporating proteins or peptides into a matrix and administration thereof through mucosa
CA2319495A1 (en) 1998-06-08 1999-12-16 Advanced Medicine, Inc. Multibinding inhibitors of microsomal triglyceride transferase protein
US6326354B1 (en) 1998-08-19 2001-12-04 Washington University Modulation of apoptosis with bid
US6194402B1 (en) 1998-09-02 2001-02-27 Merck & Co., Inc. Enhancement of return to independent living status with a growth hormone secretagogue
US7173005B2 (en) 1998-09-02 2007-02-06 Antyra Inc. Insulin and IGF-1 receptor agonists and antagonists
US6572856B1 (en) 1998-09-10 2003-06-03 The University Of Virginia Patent Foundation Methods for the prevention and treatment of cancer using anti-C3b(i) antibodies
US6696063B1 (en) 1998-12-30 2004-02-24 Applied Research Systems Ars Holding N.V. Treatment of HIV-associated dysmorphia/dysmetabolic syndrome (HADDS) with or without lipodystrophy
EP1143943B1 (en) 1999-01-29 2007-12-12 The Board Of Trustees Of The University Of Illinois USE OF p53 INHIBITORS FOR THE TREATMENT OF SIDE EFFECTS OF CANCER THERAPY
US6372490B1 (en) 1999-02-23 2002-04-16 Curagen Corporation Nucleic acid encoding the MDM interacting protein
EP1216309A2 (en) 1999-03-01 2002-06-26 Variagenics, Inc. Methods for targeting rna molecules
SK13082001A3 (sk) 1999-03-29 2002-03-05 The Procter & Gamble Company Cyklický peptidový analóg a jeho výroba
US6444425B1 (en) 1999-04-02 2002-09-03 Corixa Corporation Compounds for therapy and diagnosis of lung cancer and methods for their use
US6713280B1 (en) 1999-04-07 2004-03-30 Thomas Jefferson University Enhancement of peptide cellular uptake
DE60020554D1 (de) 1999-04-27 2005-07-07 Aionix Invest Ltd Zusatz für die wiederherstellung des wachstumhormonspiegels
US20090175821A1 (en) 1999-05-17 2009-07-09 Bridon Dominique P Modified therapeutic peptides with extended half-lives in vivo
EP1591453A1 (en) 1999-05-17 2005-11-02 ConjuChem Inc. Modified peptides yy and conjugates thereof
US7192713B1 (en) 1999-05-18 2007-03-20 President And Fellows Of Harvard College Stabilized compounds having secondary structure motifs
WO2000071096A2 (en) 1999-05-24 2000-11-30 Introgen Therapeutics, Inc. Methods and compositions for non-viral gene therapy for treatment of hyperproliferative diseases
KR20020047096A (ko) 1999-07-26 2002-06-21 크로커 사무엘 에스 초활성 돼지 성장 호르몬 분비 호르몬 유사체
US6461634B1 (en) 1999-08-20 2002-10-08 Edward Marshall Food-based delivery of HGH-stimulating and other nutritional supplements
US20080032931A1 (en) 1999-08-25 2008-02-07 Steward Lance E Activatable clostridial toxins
US6696418B1 (en) 1999-09-01 2004-02-24 Pfizer Inc. Somatostatin antagonists and agonists that act at the SST subtype 2 receptor
US20020016298A1 (en) 1999-09-01 2002-02-07 Hay Bruce A. Somatostatin antagonists and agonists that act at the sst subtype 2 receptor
US20030181367A1 (en) 1999-09-27 2003-09-25 O'mahony Daniel Conjugates of membrane translocating agents and pharmaceutically active agents
AU1925701A (en) 1999-11-22 2001-06-04 Zymogenetics Inc. Method of forming a peptide-receptor complex with zsig33
US6831155B2 (en) 1999-12-08 2004-12-14 President And Fellows Of Harvard College Inhibition of p53 degradation
US6348558B1 (en) 1999-12-10 2002-02-19 Shearwater Corporation Hydrolytically degradable polymers and hydrogels made therefrom
US6579967B1 (en) 1999-12-14 2003-06-17 The Salk Institute For Biological Studies Receptor-selective somatostatin analogs
ATE319996T1 (de) 1999-12-16 2006-03-15 Chugai Pharmaceutical Co Ltd Verfahren zum auffinden eines heilmittels für krebs mit hilfe von interaktionsdomänen von p53 und mortalin
GB0018891D0 (en) 2000-08-01 2000-09-20 Novartis Ag Organic compounds
US20010020012A1 (en) 2000-02-01 2001-09-06 Andersen Maibritt Bansholm Use of compounds for the regulation of food intake
DE10009341A1 (de) 2000-02-22 2001-09-06 Florian Kern Verfahren zur antigen-spezifischen Stimulation von T-Lymphozyten
US6495674B1 (en) 2000-02-25 2002-12-17 The Salk Institute For Biological Studies Evectins and their use
US20020002198A1 (en) 2000-04-17 2002-01-03 Parr Tyler B. Chemical synergy to elevate growth hormone release in vertebrates
US6495589B2 (en) 2000-04-28 2002-12-17 Pfizer Inc. Somatostatin antagonists and agonists that act at the SST subtype 2 receptor
US6897286B2 (en) 2000-05-11 2005-05-24 Zymogenetics, Inc. Zsig33-like peptides
CA2380423A1 (en) 2000-05-17 2001-11-22 Bionebraska, Inc. Peptide pharmaceutical formulations
ATE446758T1 (de) 2000-05-31 2009-11-15 Pfizer Prod Inc Verwendung von wachstumshormonsekretagoga zur förderung der beweglichkeit des verdauungstrakts
IL143690A0 (en) 2000-06-19 2002-04-21 Pfizer Prod Inc The use of growth hormone secretagogues to treat systemic lupus erythematosus and inflammatory bowel disease
IL143942A0 (en) 2000-06-29 2002-04-21 Pfizer Prod Inc Use of growth hormone secretagogues for treatment of physical performance decline
US7166712B2 (en) 2000-07-12 2007-01-23 Philadelphia, Health And Education Corporation Mammalian MDM2 binding proteins and uses thereof
IL144468A0 (en) 2000-07-27 2002-05-23 Pfizer Prod Inc Use of growth hormone secretagogues for improvement of functional health status
US7049290B2 (en) 2000-07-28 2006-05-23 Universität Zürich Essential downstream component of the wingless signaling pathway and therapeutic and diagnostic applications based thereon
JP2004509079A (ja) 2000-08-02 2004-03-25 セラテクノロジーズ・インコーポレーテッド 効力が増大した修飾生体ペプチド
US20040228866A1 (en) 2000-08-04 2004-11-18 Ludwig Institute For Cancer Research Suppressor genes
WO2002013833A2 (en) 2000-08-16 2002-02-21 Georgetown University Medical Center SMALL MOLECULE INHIBITORS TARGETED AT Bcl-2
IL145106A0 (en) 2000-08-30 2002-06-30 Pfizer Prod Inc Intermittent administration of a geowth hormone secretagogue
MXPA03001771A (es) 2000-08-30 2003-06-04 Pfizer Prod Inc Formulaciones de liberacion sostenida para secretagogos de hormona del crecimiento.
WO2002019963A2 (en) 2000-09-08 2002-03-14 Gryphon Therapeutics, Inc. Synthetic erythropoiesis stimulating proteins
US6720330B2 (en) 2000-11-17 2004-04-13 Pfizer Inc. Somatostatin antagonists and agonists that act at the SST subtype 2 receptor
US20030074679A1 (en) 2000-12-12 2003-04-17 Schwartz Robert J. Administration of nucleic acid sequence to female animal to enhance growth in offspring
WO2002064790A2 (en) 2000-12-19 2002-08-22 The Johns Hopkins University Jfy1 protein induces rapid apoptosis
US20020091090A1 (en) 2000-12-28 2002-07-11 Cole Bridget M. Somatostatin antagonists and agonists
CU23157A1 (es) 2001-01-03 2006-07-18 Ct Ingenieria Genetica Biotech COMPOSICION FARMACéUTICA PARA EL TRATAMIENTO DEL DANO TISULAR DEBIDO A FALTA DE IRRIGACION SANGUINEA ARTERIAL
BR0206919A (pt) 2001-02-02 2004-07-06 Conjuchem Inc Derivados de fator de liberação de hormÈnio de crescimento de longa duração
EP1363934B1 (en) 2001-02-23 2014-10-08 Polyphor Ltd. Template-fixed peptidomimetics with antimicrobial activity
GB0104588D0 (en) 2001-02-24 2001-04-11 Univ Dundee Novel p-53 inducible protein
DE10109813A1 (de) 2001-03-01 2002-09-12 Thomas Stanislawski Tumor-Peptidantigen aus humanem mdm2 Proto-Onkogen
US20050054770A1 (en) 2001-03-09 2005-03-10 Spatola Arno F. Helicomimetics and stabilized lxxll peptidomimetics
US7019109B2 (en) 2001-03-16 2006-03-28 The Salk Institute For Bilogical Studies SSTR1-selective analogs
RU2259375C2 (ru) 2001-04-09 2005-08-27 Дзе Администрейторс Оф Дзе Тьюлейн Эдьюкейшнл Фанд Агонисты соматостатина
US6368617B1 (en) 2001-05-15 2002-04-09 Reliv' International, Inc. Dietary supplement
DE60227802D1 (de) 2001-06-05 2008-09-04 Elan Pharma Int Ltd Mahlvorrichtung und verfahren zu deren betrieb
JP2004536834A (ja) 2001-07-06 2004-12-09 オークランド ユニサーヴィシズ リミテッド 高血圧症治療
WO2003014056A1 (en) 2001-08-08 2003-02-20 Kaneka Corporation Process for producing optically active 2-substituted carboxylic acid
US20040106548A1 (en) 2001-09-07 2004-06-03 Schmidt Michelle A Conformationally constrained labeled peptides for imaging and therapy
AU2002324909A1 (en) 2001-09-07 2003-03-24 Baylor College Of Medicine Linear dna fragments for gene expression
US20020045192A1 (en) 2001-09-19 2002-04-18 St. Jude Children's Research Hospital Arf and HDM2 interaction domains and methods of use thereof
EP1312363A1 (en) 2001-09-28 2003-05-21 Pfizer Products Inc. Methods of treatment and kits comprising a growth hormone secretagogue
US20030083241A1 (en) 2001-11-01 2003-05-01 Young Charles W. Use of somatostatin receptor agonists in the treatment of human disorders of sleep hypoxia and oxygen deprivation
CN101157924A (zh) 2001-12-11 2008-04-09 人体基因组科学有限公司 嗜中性白细胞因子α
EP1321474A1 (en) 2001-12-18 2003-06-25 Universite De Geneve A method for inducing apoptosis
EP1455814B1 (en) 2001-12-18 2012-04-04 Alizé Pharma SAS Pharmaceutical compositions comprising unacylated ghrelin for use in the treatment of insulin resistance
AU2002367160A1 (en) 2001-12-24 2003-07-15 Auckland Uniservices Limited Therapy for growth hormone induced insulin resistance in juveniles with growth disorders
WO2003057158A2 (en) 2001-12-31 2003-07-17 Dana-Farber Cancer Institute, Inc. Method of treating apoptosis and compositions thereof
WO2003059933A2 (en) 2002-01-03 2003-07-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem Conformationally constrained c-backbone cyclic peptides
EP1499731B1 (en) 2002-02-07 2011-05-25 Baylor College Of Medicine Modified pituitary gland development in offspring from expectant mother animals treated with growth hormone releasing hormone therapy
JP2006514914A (ja) 2002-02-14 2006-05-18 バイエル・フアーマシユーチカルズ・コーポレーシヨン 有機溶媒中および乾燥状態のペプチドの安定化における製剤の戦略
WO2003070892A2 (en) 2002-02-15 2003-08-28 The Regents Of The University Of Michigan Inhibitors of rgs proteins
US20030166138A1 (en) 2002-02-21 2003-09-04 Todd Kinsella Cyclic peptides and analogs useful to treat allergies
JP2005520562A (ja) 2002-03-26 2005-07-14 バイエル・ヘルスケア・アクチェンゲゼルシャフト 成長ホルモン分泌促進受容体（ｇｈｓ）に関連する疾患の診断および治療
US7498134B2 (en) 2002-03-30 2009-03-03 The Trustees Of Columbia University In The City Of New York HAUSP-Mdm2 interaction and uses thereof
US7524630B2 (en) 2002-04-22 2009-04-28 University Of Florida Research Foundation, Inc. Functionalized nanoparticles and methods of use
AU2003235504A1 (en) 2002-05-13 2003-11-11 3-Dimensional Pharmaceuticals, Inc. Method for cytoprotection through mdm2 and hdm2 inhibition
AU2003247435A1 (en) 2002-05-30 2003-12-19 European Molecular Biology Laboratory Combinatorial chemical library ii
US7375234B2 (en) 2002-05-30 2008-05-20 The Scripps Research Institute Copper-catalysed ligation of azides and acetylenes
US7208154B2 (en) 2002-06-03 2007-04-24 Regents Of The University Of Michigan Methods and compositions for the treatment of MHC-associated conditions
SE0201863D0 (en) 2002-06-18 2002-06-18 Cepep Ab Cell penetrating peptides
WO2004007675A2 (en) 2002-07-15 2004-01-22 The Johns Hopkins University Neuronal and optic nerve gene expression patterns
AU2003259172A1 (en) 2002-07-24 2004-02-09 The Salk Institute For Biological Studies Receptor (sstr4)- selective somatostatin analogs
AU2003261774A1 (en) 2002-09-06 2004-03-29 Kaneka Corporation PROCESS FOR PRODUCING L-Alpha-METHYLCYSTEINE DERIVATIVE
US20040171809A1 (en) 2002-09-09 2004-09-02 Korsmeyer Stanley J. BH3 peptides and method of use thereof
EP1539959A2 (en) 2002-09-18 2005-06-15 Centre Hospitalier de l'Université de Montréal (CHUM) Ghrh analogues
WO2004026896A2 (en) 2002-09-23 2004-04-01 Medivir Ab Hcv ns-3 serine protease inhibitors
ATE537186T1 (de) 2002-10-07 2011-12-15 Ludwig Inst For Cancer Res Ltd P53-bindendes polypeptid
EP1556084A4 (en) 2002-10-07 2006-01-11 Zymogenetics Inc METHOD FOR REGULATING THE BODY WEIGHT
EP1407779A1 (en) 2002-10-10 2004-04-14 Gastrotech A/S Use of ghrelin for treating low body weight and body fat in gastrectomized individuals
CA2814266A1 (en) 2002-10-24 2004-05-06 Dow Global Technologies Llc Stabilization of olefin metathesis product mixtures
EP2135867B1 (en) 2002-11-07 2013-09-25 Kosan Biosciences Incorporated Trans-9, 10-dehydroepothilone C and trans-9, 10-dehydroepothilone D, analogs thereof and methods of making the same
JP4551222B2 (ja) 2002-11-08 2010-09-22 エフ．ホフマン−ラ ロシュ アーゲー Ｐｐａｒアゴニストとしての置換４−アルコキシオキサゾール誘導体
US20050227932A1 (en) 2002-11-13 2005-10-13 Tianbao Lu Combinational therapy involving a small molecule inhibitor of the MDM2: p53 interaction
US7166575B2 (en) 2002-12-17 2007-01-23 Nastech Pharmaceutical Company Inc. Compositions and methods for enhanced mucosal delivery of peptide YY and methods for treating and preventing obesity
WO2004056869A1 (en) 2002-12-20 2004-07-08 7Tm Pharma A/S Ghrelin receptor inverse agonist for regulation of feeding behaviours
US20070032417A1 (en) 2002-12-24 2007-02-08 Walter And Eliza Hall Institute Of Medical Research Peptides and therapeutic uses thereof
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
US20040204358A1 (en) 2003-01-28 2004-10-14 Advisys, Inc. Reducing culling in herd animals growth hormone releasing hormone (GHRH)
WO2004067548A2 (en) 2003-01-31 2004-08-12 Theratechnologies Inc. Chemically modified metabolites of regulatory peptides and methods of producing and using same
WO2004073729A1 (ja) 2003-02-21 2004-09-02 Translational Research Ltd. 薬物の経鼻投与用組成物
CA2517285A1 (en) 2003-02-26 2004-09-10 Japan Science And Technology Agency Transcriptional factor inducing apoptosis in cancer cell
EP1452868A2 (en) 2003-02-27 2004-09-01 Pepscan Systems B.V. Method for selecting a candidate drug compound
US20070060512A1 (en) 2003-03-04 2007-03-15 Homayoun Sadeghi Dipeptidyl-peptidase protected protein
WO2004084943A1 (en) 2003-03-19 2004-10-07 Beth Israel Deaconess Medical Center, Inc Use of antagonists of ghrelin or ghrelin receptor to treat intestinal inflammation
US7632920B2 (en) 2003-04-10 2009-12-15 Schering Corporation Soluble, stable form of HDM2, crystalline forms thereof and methods of use thereof
CN102174102A (zh) 2003-05-15 2011-09-07 塔夫茨大学信托人 肽和多肽药物的稳定类似物
WO2004105789A1 (en) 2003-05-29 2004-12-09 Theratechnologies Inc. Grf analog compositions and their use
AU2003902743A0 (en) 2003-06-02 2003-06-19 Promics Pty Limited Process for the preparation of cyclic peptides
WO2005004895A2 (en) 2003-06-09 2005-01-20 Nastech Pharmaceutical Company Inc. Compositions and methods for enhanced mucosal delivery of growth hormone
US7476653B2 (en) 2003-06-18 2009-01-13 Tranzyme Pharma, Inc. Macrocyclic modulators of the ghrelin receptor
USRE42013E1 (en) 2003-06-18 2010-12-28 Tranzyme Pharma Inc. Macrocyclic modulators of the ghrelin receptor
US20090198050A1 (en) 2003-06-18 2009-08-06 Tranzyme Pharma Inc. Macrocyclic Modulators of the Ghrelin Receptor
US7491695B2 (en) 2003-06-18 2009-02-17 Tranzyme Pharma Inc. Methods of using macrocyclic modulators of the ghrelin receptor
USRE42624E1 (en) 2003-06-18 2011-08-16 Tranzyme Pharma Inc. Methods of using macrocyclic modulators of the ghrelin receptor
US7521420B2 (en) 2003-06-18 2009-04-21 Tranzyme Pharma, Inc. Macrocyclic antagonists of the motilin receptor
WO2005000876A2 (en) 2003-06-27 2005-01-06 Proteologics, Inc. Ring finger family proteins and uses related thereto
WO2005007675A2 (en) 2003-07-09 2005-01-27 The Scripps Research Institute TRIAZOLE &epsiv;-AMINO ACIDS
US20070185031A1 (en) 2003-07-14 2007-08-09 Northwestern University Reducing polyglutamine-based aggregation
GB0317815D0 (en) 2003-07-30 2003-09-03 Amersham Health As Imaging agents
CA2536112A1 (en) 2003-08-20 2005-03-03 Northern Sydney And Central Coast Area Health Service Methods for enhancing embryo viability
US7968080B2 (en) 2003-08-20 2011-06-28 The Regents Of The University Of California Somatostatin analogs with inhibitory activity to growth hormone release
WO2005027913A1 (en) 2003-09-19 2005-03-31 Pfizer Products Inc. Pharmaceutical compositions and methods comprising combinations of 2-alkylidene-19-nor-vitamin d derivatives and a growth hormone secretagogue
PT1670444E (pt) 2003-10-03 2011-07-29 Veijlen N V Utilização de derivados do ácido indoleacético que aumentam o nível de igf-1 no soro para a preparação de uma composição terapêutica para o tratamento de várias doenças
CA2540452A1 (en) 2003-10-03 2005-04-14 Merck & Co., Inc. Benzylether and benzylamino beta-secretase inhibitors for the treatment of alzheimer's disease
GB0323728D0 (en) 2003-10-10 2003-11-12 Royal College Of Surgeons Ie Peptidomimetics and uses thereof
EP1673386A2 (en) 2003-10-16 2006-06-28 AplaGen GmbH Stabilized alpha-helical peptides
WO2005044840A2 (en) 2003-10-17 2005-05-19 The Cbr Institute For Biomedical Research, Inc. Modulation of anergy and methods for isolating anergy-modulating compounds
US7893025B2 (en) 2003-10-20 2011-02-22 Theratechnolgies Inc. Use of growth hormone releasing factor analogs in treating patients suffering from wasting
US7273927B2 (en) 2003-11-03 2007-09-25 University Of Massachusetts Mdm2 splice variants
PL2332968T3 (pl) 2003-11-05 2017-08-31 Dana-Farber Cancer Institute, Inc. Stabilizowane alfa-helikalne peptydy i ich zastosowania
US20050147581A1 (en) 2003-11-19 2005-07-07 The Board Of Trustees Of The University Of Illinois Macromolecular drug complexes having improved stability and therapeutic use of the same
WO2005074521A2 (en) 2004-01-30 2005-08-18 The Trustees Of Columbia University In The City Of New York C-TERMINAL p53 PALINDROMIC PEPTIDE THAT INDUCES APOPTOSIS OF CELLS WITH ABERRANT p53 AND USES THEREOF
US20070161551A1 (en) 2004-02-10 2007-07-12 De Luca Giampiero Methods and compositions for the treatment of lipodystrophy
GB0404731D0 (en) 2004-03-03 2004-04-07 Indp Administrative Inst Nims Method and products for the selective degradation of proteins
US20050203009A1 (en) 2004-03-12 2005-09-15 Bayer Pharmaceuticals Corporation VPAC1 selective antagonists and their pharmacological methods of use
WO2005090388A1 (en) 2004-03-19 2005-09-29 The University Of Queensland Alpha helical mimics, their uses and methods for their production
JP2007531769A (ja) 2004-03-30 2007-11-08 サファイア セラピューティクス インコーポレイテッド 成長ホルモン分泌促進薬を使用したｃ−反応性蛋白質の低減方法
JP2007532495A (ja) 2004-04-07 2007-11-15 ガストロテック・ファルマ・アクティーゼルスカブ グレリン欠乏を治療するための分泌促進薬の使用
US7034050B2 (en) 2004-04-28 2006-04-25 Romano Deghenghi Pseudopeptides growth hormone secretagogues
BRPI0511328A (pt) 2004-05-18 2007-12-04 Hoffmann La Roche cis-imidazolinas
US7202332B2 (en) 2004-05-27 2007-04-10 New York University Methods for preparing internally constrained peptides and peptidomimetics
CN100335467C (zh) 2004-06-04 2007-09-05 中国科学院上海有机化学研究所 一锅法区域选择性合成5－碘代－1，4－二取代－1，2，3－三氮唑化合物
EP1602663A1 (en) 2004-06-04 2005-12-07 Chiralix B.V. Triazole-linked glycoamino acids and glycopeptides
US7501397B2 (en) 2004-06-04 2009-03-10 The Brigham And Women's Hospital, Inc. Helical peptidomimetics with enhanced activity
US7893278B2 (en) 2004-06-17 2011-02-22 Hoffman-La Roche Inc. CIS-imidazolines
US7632924B2 (en) 2004-06-18 2009-12-15 Ambrx, Inc. Antigen-binding polypeptides and their uses
WO2006010118A2 (en) 2004-07-09 2006-01-26 The Regents Of The University Of Michigan Conformationally constrained smac mimetics and the uses thereof
US8039456B2 (en) 2004-08-12 2011-10-18 Helsinn Therapeutics (U.S.), Inc. Method of stimulating the motility of the gastrointestinal system using ipamorelin
KR101380200B1 (ko) 2004-08-12 2014-04-01 헬신 세라퓨틱스 (유.에스.) 인크. 성장 호르몬 분비촉진제를 이용하여 위장관계의 운동성을촉진하는 방법
US20080261873A1 (en) 2004-08-18 2008-10-23 Elixir Pharmaceuticals, Inc. Growth-Hormone Secretagogues
US7151084B2 (en) 2004-12-27 2006-12-19 Miller Landon C G Compound and method of treating neurogenic conditions using non-steroidal anti-inflammatory drug complexes
US7074775B2 (en) 2004-09-14 2006-07-11 Miller Landon C G Aminobutyramide conjugate and a pharmaceutical composition for treatment of neuronal disorders
US7157421B2 (en) 2004-12-27 2007-01-02 Miller Landon C G Piracetam and piracetam analog conjugate and a pharmaceutical composition for treatment of neuronal disorders
US7402652B2 (en) 2004-09-14 2008-07-22 Miller Landon C G Baclofen conjugate and a pharmaceutical composition for treatment of neuronal disorders
US9598470B2 (en) 2004-10-07 2017-03-21 Craig W. Beattie Compositions and methods to prevent cancer by stabilizing P53 through non MDM2-mediated pathways
AU2005297366B2 (en) 2004-10-20 2011-12-08 Theratechnologies Inc. GH secretagogues and uses thereof
WO2006050034A1 (en) 2004-10-29 2006-05-11 Schering Corporation Substituted 5-carboxyamide pyrazoles and [1,2,4]triazoles as antiviral agents
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
US20060148715A1 (en) 2004-12-20 2006-07-06 Baylor College Of Medicine Structural requirements for STAT3 binding and recruitment to phosphotyrosine ligands
GB0428187D0 (en) 2004-12-23 2005-01-26 Univ Liverpool Cancer treatment
NZ560504A (en) 2005-01-24 2009-07-31 Pepscan Systems Bv Binding compounds, immunogenic compounds and peptidomimetics
FR2881430B1 (fr) 2005-02-01 2010-10-22 Servier Lab Nouveaux peptides interagissant avec les membres anti-apoptotiques de la famille de proteines bcl-2 et utilisations
US7759383B2 (en) 2005-02-22 2010-07-20 The Regents Of The University Of Michigan Small molecule inhibitors of MDM2 and the uses thereof
EP2316847A3 (en) 2005-03-15 2012-01-18 Allergan, Inc. Modified clostridial toxins with enhanced targeting capabilities for endogenous clostridial toxin receptor systems
WO2006103666A2 (en) 2005-03-28 2006-10-05 Yeda Research And Development Co. Ltd. Isolated bid polypeptides, polynucleotides encoding same and antibodies directed thereagainst and methods of using same for inducing cell cycle arrest or apoptosis
US8324153B2 (en) 2008-05-06 2012-12-04 New York Blood Center, Inc. Antiviral cell-penetrating peptides
WO2006122931A1 (en) 2005-05-20 2006-11-23 Biovitrum Ab (Publ) Beta-carboline derivatives and theri use as ghsr modulators
US20090275648A1 (en) 2005-06-13 2009-11-05 Fraser Graeme L Macrocyclic ghrelin receptor antagonists and inverse agonists and methods of using the same
US20070020620A1 (en) 2005-07-14 2007-01-25 Finn M G Compositions and methods for coupling a plurality of compounds to a scaffold
JP2009501731A (ja) 2005-07-21 2009-01-22 ニューサウス イノベーションズ ピティ リミテッド 癌を治療する方法
CA2616396C (en) 2005-07-22 2013-07-23 Societe De Conseils De Recherches Et D'applications Scientifiques (S.C.R Growth hormone secretagogues
EP1757290A1 (en) 2005-08-16 2007-02-28 Zentaris GmbH Novel triazole derivatives as ghrelin analogue ligands of growth hormone secretagogue receptors
CN105111302A (zh) 2005-09-28 2015-12-02 益普生制药股份有限公司 生长素释放肽类似物
US20070161544A1 (en) 2006-01-06 2007-07-12 Peter Wipf Selective targeting agents for mitcochondria
US20110143992A1 (en) 2006-02-13 2011-06-16 Dennis Taub Methods and Compositions Related to GHS-R Antagonists
US7745573B2 (en) 2006-02-17 2010-06-29 Polychip Pharmaceuticals Pty Ltd. Conotoxin analogues and methods for synthesis of intramolecular dicarba bridge-containing peptides
US7538190B2 (en) 2006-02-17 2009-05-26 Polychip Pharmaceuticals Pty Ltd Methods for the synthesis of two or more dicarba bridges in organic compounds
GB0603295D0 (en) 2006-02-18 2006-03-29 Ardana Bioscience Ltd Methods and kits
CU23592A1 (es) 2006-02-28 2010-11-11 Ct Ingenieria Genetica Biotech Método para prevenir y eliminar las fibrosis y otras formas de depósito patológico en los tejidos aplicando el péptido secretagogo ghrp-6
AU2007227641B2 (en) 2006-03-13 2012-11-29 Liat Mintz Use of ghrelin splice variant for treating cachexia and/or anorexia and/or anorexia-cachexia and/or malnutrition and/or lipodystrophy and/or muscle wasting and/or appetite stimulation
JP2010523466A (ja) 2006-04-13 2010-07-15 グラクソ グループ リミテッド 成長ホルモン分泌促進因子受容体アゴニストとしてのアリールおよびヘテロアリールスルホンアミド類
WO2007127457A2 (en) 2006-04-28 2007-11-08 The Administrators Of The Tulane Educational Fund Ghrelin/growth hormone releasing peptide/growth hormone secretatogue receptor antagonists and uses thereof
GB0611405D0 (en) 2006-06-09 2006-07-19 Univ Belfast FKBP-L: A novel inhibitor of angiogenesis
US8226949B2 (en) 2006-06-23 2012-07-24 Aegis Therapeutics Llc Stabilizing alkylglycoside compositions and methods thereof
US8084022B2 (en) 2006-06-23 2011-12-27 Aegis Therapeutics, Llc Stabilizing alkylglycoside compositions and methods thereof
US7998927B2 (en) 2006-06-23 2011-08-16 Aegis Therapeutics, Llc Stabilizing alkylglycoside compositions and methods thereof
US7425542B2 (en) 2006-06-23 2008-09-16 Aegis Therapeutics, Inc. Stabilizing alkylglycoside compositions and methods thereof
US20090326193A1 (en) 2006-06-23 2009-12-31 Aegis Therapeutics Llc Stabilizing alkylglycoside compositions and methods thereof
US8173594B2 (en) 2006-06-23 2012-05-08 Aegis Therapeutics, Llc Stabilizing alkylglycoside compositions and methods thereof
KR20090042779A (ko) 2006-06-30 2009-04-30 쉐링 코포레이션 Ｐ53 활성을 증가시키는 치환된 피페리딘 및 이의 사용
US8088733B2 (en) 2006-07-06 2012-01-03 Tranzyme Pharma Inc. Methods of using macrocyclic agonists of the ghrelin receptor for treatment of gastrointestinal motility disorders
EP2051725A4 (en) 2006-07-11 2011-06-15 Harkness Pharmaceuticals Inc METHOD FOR THE TREATMENT OF GRAIN WITH SATURATION FACTORS
EP2052253A2 (en) 2006-07-26 2009-04-29 Pepscan Systems B.V. Immunogenic compounds and protein mimics
WO2008014216A1 (en) 2006-07-28 2008-01-31 St. Jude Children's Research Hospital Method for treating ocular cancer
US7737174B2 (en) 2006-08-30 2010-06-15 The Regents Of The University Of Michigan Indole inhibitors of MDM2 and the uses thereof
DE602007010664D1 (de) 2006-09-04 2010-12-30 Univ Dundee P53 aktivierende benzoylharnstoff- und benzoylthioharnstoff-verbindungen
WO2008033557A2 (en) 2006-09-15 2008-03-20 Siemens Medical Solutions Usa, Inc. Click chemistry-derived cyclic peptidomimetics as integrin markers
JP2010504298A (ja) 2006-09-21 2010-02-12 エフ．ホフマン−ラ ロシュ アーゲー オキシンドール誘導体
US7897394B2 (en) 2006-09-21 2011-03-01 Intrexon Corporation Endoplasmic reticulum localization signals
BRPI0717169C1 (pt) 2006-09-27 2012-12-04 Ipsen Pharma Sas análogos de grelina substituìda no n-terminal
ES2387827T3 (es) 2006-10-05 2012-10-02 New York Blood Center, Inc. Péptidos antivirales helicoidales, de pequeño tamaño, terapéuticos estabilizados
KR100860060B1 (ko) 2006-10-12 2008-09-24 한국과학기술연구원 ＨＩＦ-１α Ｃ-말단 펩타이드와 ＣＢＰ 또는 ｐ３００단백질과의 결합을 정량분석하는 방법 및 상기 방법을이용한 단백질 복합체 형성을 저해하는 억제제의 스크리닝방법
US8691761B2 (en) 2006-10-16 2014-04-08 Jean E. F. Rivier Somatostatin receptor 2 antagonists
SI2076535T1 (sl) 2006-10-16 2013-07-31 The Salk Institute For Biological Studies Antagonisti somatostatina, selektivni za receptor (SSTR2)
EP2997973A1 (en) 2006-11-15 2016-03-23 Dana-Farber Cancer Institute, Inc. Stabalized maml peptides and uses thereof
US7932397B2 (en) 2006-11-22 2011-04-26 Massachusetts Institute Of Technology Olefin metathesis catalysts and related methods
CN105061577A (zh) 2006-12-14 2015-11-18 爱勒让治疗公司 双巯基大环化系统
US7981998B2 (en) 2006-12-14 2011-07-19 Aileron Therapeutics, Inc. Bis-sulfhydryl macrocyclization systems
EP2121007A1 (en) 2006-12-21 2009-11-25 Cytos Biotechnology AG Circular ccr5 peptide conjugates and uses thereof
WO2008092281A1 (en) 2007-01-29 2008-08-07 Polyphor Ltd. Template-fixed peptidomimetics
US8889632B2 (en) 2007-01-31 2014-11-18 Dana-Farber Cancer Institute, Inc. Stabilized p53 peptides and uses thereof
CA2677399C (en) 2007-02-09 2016-09-13 Tranzyme Pharma, Inc. Macrocyclic ghrelin receptor modulators and methods of using the same
CN109627287A (zh) 2007-02-23 2019-04-16 爱勒让治疗公司 三唑大环系统
WO2008106507A2 (en) 2007-02-27 2008-09-04 University Of South Florida Mdm2/mdmx inhibitor peptide
WO2008112941A1 (en) 2007-03-13 2008-09-18 Board Of Regents The University Of Texas System Composition and method for the treatment of diseases affected by a peptide receptor
CN101730708B (zh) 2007-03-28 2013-09-18 哈佛大学校长及研究员协会 缝合多肽
TWI429436B (zh) 2007-04-10 2014-03-11 Helsinn Therapeutics Us Inc 使用生長激素促泌素治療或預防嘔吐之方法
US20080260820A1 (en) 2007-04-19 2008-10-23 Gilles Borrelly Oral dosage formulations of protease-resistant polypeptides
US10716828B2 (en) 2007-05-02 2020-07-21 Dana-Farber Cancer Institute, Inc. Methods of modulating cellular homeostatic pathways and cellular survival
EP2150537A4 (en) 2007-06-01 2010-09-22 Acologix Inc STABLE PEPTIDE FORMULATION AT HIGH TEMPERATURE
WO2009009727A2 (en) 2007-07-12 2009-01-15 Akela Pharma Srl Ghrh analogs and therapeutic uses thereof
RU2007133287A (ru) 2007-09-05 2009-03-10 Ионов Иль Давидович (RU) Противопсориатическое средство и способ его применения (варианты)
EP2187950A1 (en) 2007-09-11 2010-05-26 Mondobiotech Laboratories AG Use of insulin c-peptide, alone or in combination with glp-1, as a therapeutic agent
AU2008303955A1 (en) 2007-09-11 2009-04-02 Mondobiotech Laboratories Ag Use of melanocyte-stimulating hormone release-inhibiting factor as a therapeutic agent in the treatment of Pseudomonas aeruginosa infection
KR20100056523A (ko) 2007-09-11 2010-05-27 몬도바이오테크 래보래토리즈 아게 치료제로서의 ｇｒｆ-1 (1-29) 및 코르티코트로핀-방출 인자의 용도
US20100298393A1 (en) 2007-09-17 2010-11-25 Peter Vanderklish Modulation of Growth Hormone, DHEA, and Cortisol with Positive Modulators of AMPA Type Glutamate Receptors
WO2009042237A2 (en) 2007-09-26 2009-04-02 Dana Farber Cancer Institute Methods and compositions for modulating bcl-2 family polypeptides
SI2225271T1 (sl) 2007-12-03 2013-10-30 Italfarmaco S.P.A. Novi selektivni analogi somatostatina
JP5653219B2 (ja) 2007-12-31 2015-01-14 ニューヨーク ユニバーシティ 水素結合サロゲートをベースとする人工ヘリックスによるウイルス宿主膜融合の制御
SI2235064T1 (sl) 2008-01-07 2016-04-29 Amgen Inc. Metoda za izdelavo heterodimernih molekul - protitelesa fc z uporabo elektrostatičnih usmerjevalnih učinkov
DK2245464T3 (en) 2008-01-25 2017-02-20 Multivir Inc P53 BIOMARKETS
AU2009206684C1 (en) 2008-01-25 2014-12-04 Massachusetts Institute Of Technology Catalysts for metathesis reactons including enantioselective olefin metathesis, and related methods
US20090275519A1 (en) 2008-02-08 2009-11-05 Aileron Therapeutics, Inc. Therapeutic peptidomimetic macrocycles
US20110144303A1 (en) 2008-04-08 2011-06-16 Aileron Therapeutics, Inc. Biologically Active Peptidomimetic Macrocycles
US20110250685A1 (en) 2008-06-03 2011-10-13 Nash Huw M Compositions and methods for enhancing cellular transport of biomolecules
US8343760B2 (en) 2008-06-05 2013-01-01 University Of Maryland, Baltimore p53 activator peptides
EP2310028B1 (en) 2008-06-12 2016-11-16 Ipsen Bioinnovation Limited Fusion proteins for use in the treatment of acromegaly
US8796216B2 (en) 2008-06-12 2014-08-05 Syntaxin Limited Suppression of neuroendocrine diseases
AU2009259034B2 (en) 2008-06-12 2013-10-31 Ipsen Bioinnovation Limited Suppression of cancers
US20110158973A1 (en) 2008-06-12 2011-06-30 Syntaxin Limited Suppression of cancers
WO2009158395A1 (en) 2008-06-25 2009-12-30 Braasch Biotech Llc Compositions and methods for enhanced somatostatin immunogenicity
WO2010011313A2 (en) 2008-07-23 2010-01-28 President And Fellows Of Harvard College Ligation of stapled polypeptides
GB0813873D0 (en) 2008-07-30 2008-09-03 Univ Dundee Compounds
WO2010033617A2 (en) * 2008-09-16 2010-03-25 The Research Foundation Of State University Of New York Stapled peptides and method of synthesis
CA2737614A1 (en) 2008-09-18 2010-03-25 New York University Inhibiting interaction between hif-1.alpha. and p300/cbp with hydrogen bond surrogate-based helices
CA2737040C (en) 2008-09-19 2017-05-02 Nektar Therapeutics Polymer conjugates of therapeutic peptides
CN102482336B (zh) 2008-09-22 2015-05-06 爱勒让治疗公司 拟肽大环化合物
EP2331567A1 (en) * 2008-09-22 2011-06-15 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
AU2009294871A1 (en) 2008-09-22 2010-03-25 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
AU2009294874A1 (en) 2008-09-22 2010-03-25 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
AU2009294869A1 (en) * 2008-09-22 2010-03-25 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
CA2737921C (en) 2008-09-22 2019-01-15 Aileron Therapeutics, Inc. Methods for preparing purified alpha-helical peptidomimetic macrocycle compositions with low metal ppm levels
US20110218155A1 (en) 2008-10-10 2011-09-08 Dana-Farber Cancer Institute, Inc. Chemical modulators of pro-apoptotic bax and bcl-2 polypeptides
JP2010120881A (ja) 2008-11-19 2010-06-03 Keio Gijuku ヒト癌タンパク質ＭＤＭ２とヒト癌抑制タンパク質ｐ５３との相互作用阻害ペプチド及びその使用
KR101298168B1 (ko) 2008-11-21 2013-08-20 충남대학교산학협력단 스네일―ｐ５３ 간의 결합을 저해하는 화합물 및 이를 유효성분으로 함유하는 암질환 치료제
US9458202B2 (en) 2008-11-24 2016-10-04 Aileron Therapeutics, Inc. Peptidomimetic macrocycles with improved properties
WO2010065572A1 (en) 2008-12-04 2010-06-10 The Salk Institute For Biological Studies Sstr1-selective analogs
EP2358748B1 (en) 2008-12-09 2016-07-06 Dana-Farber Cancer Institute, Inc. Methods and compositions for specific modulation of mcl-1
US20100152114A1 (en) 2008-12-12 2010-06-17 Univ Of Miami And Usa By Dept Of Veterans Affairs Antioxidant activity of GH-RH Antagonists
JP2012515172A (ja) 2009-01-14 2012-07-05 エルロン・セラピューティクス・インコーポレイテッド ペプチド模倣大環状分子
WO2010083501A2 (en) 2009-01-16 2010-07-22 University Of South Florida Alpha-helix mimetic using a 2,5-oligopyrimidine scaffold
US8217051B2 (en) 2009-02-17 2012-07-10 Hoffmann-La Roche Inc. Spiroindolinone derivatives
US20100239589A1 (en) 2009-02-23 2010-09-23 Salk Institute For Biological Studies Methods and Compositions for Ameliorating Diabetes and Symptoms Thereof
FR2942798B1 (fr) 2009-03-05 2011-04-08 Centre Nat Rech Scient Peptides utilisables pour le traitement de la leucemie lymphoide chronique
WO2010107485A1 (en) 2009-03-17 2010-09-23 The Trustees Of Columbia University In The City Of New York E3 ligase inhibitors
US20100267636A1 (en) 2009-04-20 2010-10-21 Theratechnologies Inc. Use of cytochrome p450-metabolized drugs and grf molecules in combination therapy
US8076482B2 (en) 2009-04-23 2011-12-13 Hoffmann-La Roche Inc. 3,3′-spiroindolinone derivatives
US8883721B2 (en) 2009-05-12 2014-11-11 Mcgill University Methods of inhibiting the ghrelin/growth hormone secretatogue receptor pathway and uses thereof
US20100303791A1 (en) 2009-05-29 2010-12-02 Allergan, Inc. Methods of Treating Chronic Neurogenic Inflammation Using Glucagon Like Hormone Retargeted Endopepidases
US20100303794A1 (en) 2009-05-29 2010-12-02 Allergan, Inc. Methods of Treating Urogenital-Neurological Disorders Using Glucagon Like Hormone Retargeted Endopepidases
WO2011005219A1 (en) 2009-07-07 2011-01-13 Agency For Science, Technology And Research Novel mdm2 binding peptides and uses thereof
CN102510755A (zh) 2009-07-13 2012-06-20 哈佛大学校长及研究员协会 双功能钉接多肽和其用途
BR112012008075A2 (pt) 2009-08-26 2016-03-01 Novartis Ag compostos de heteroarila tetrassubstituídos e seu uso como moduladores de mdm2 e/ou mdm4
CA2774973A1 (en) 2009-09-22 2011-03-31 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
KR20120081166A (ko) 2009-09-30 2012-07-18 트랜자임 파르마 인크 매크로시클릭 그렐린 수용체 효능제의 염, 용매화물 및 약제학적 조성물 및 이를 사용하는 방법
US8017607B2 (en) 2009-10-14 2011-09-13 Hoffmann-La Roche Inc. N-substituted-pyrrolidines as inhibitors of MDM2-P-53 interactions
BR112012008831A2 (pt) 2009-10-14 2019-09-24 Aileron Therapeutics Inc macrociclos de peptideomiméticos aprimorados.
US20110105389A1 (en) 2009-10-30 2011-05-05 Hoveyda Hamid R Macrocyclic Ghrelin Receptor Antagonists and Inverse Agonists and Methods of Using the Same
US20120283269A1 (en) 2009-11-04 2012-11-08 Health Research Inc. Method and Compositions for Suppression of Aging
KR20120099462A (ko) 2009-11-12 2012-09-10 더 리젠츠 오브 더 유니버시티 오브 미시건 스피로-옥신돌 ｍｄｍ2 길항제
US20110118283A1 (en) 2009-11-17 2011-05-19 Qingjie Ding Substituted Pyrrolidine-2-Carboxamides
US8088815B2 (en) 2009-12-02 2012-01-03 Hoffman-La Roche Inc. Spiroindolinone pyrrolidines
CU24130B1 (es) 2009-12-22 2015-09-29 Novartis Ag Isoquinolinonas y quinazolinonas sustituidas
US8440693B2 (en) 2009-12-22 2013-05-14 Novartis Ag Substituted isoquinolinones and quinazolinones
US8658170B2 (en) 2010-01-06 2014-02-25 Joseph P. Errico Combination therapy with MDM2 and EFGR inhibitors
KR101220516B1 (ko) 2010-01-21 2013-01-10 연세대학교 산학협력단 항－ｍｄｍ２를 발현하는 인간 성체줄기세포 및 그의 용도
EP2534140B1 (en) 2010-02-08 2018-04-11 Trustees of Boston College Efficient methods for z- or cis-selective cross-metathesis
US8288431B2 (en) 2010-02-17 2012-10-16 Hoffmann-La Roche Inc. Substituted spiroindolinones
WO2011106650A2 (en) 2010-02-27 2011-09-01 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Novel p53-mdm2/p53-mdm4 antagonists to treat proliferative disease
WO2011127058A2 (en) 2010-04-09 2011-10-13 The Regents Of The University Of Michigan Biomarkers for mdm2 inhibitors for use in treating disease
US9782454B2 (en) 2010-04-22 2017-10-10 Longevity Biotech, Inc. Highly active polypeptides and methods of making and using the same
WO2011143208A1 (en) 2010-05-13 2011-11-17 Indiana University Research And Technology Corporation Glucagon superfamily peptides exhibiting g protein-coupled receptor activity
KR20130062931A (ko) 2010-05-13 2013-06-13 인디애나 유니버시티 리서치 앤드 테크놀로지 코퍼레이션 핵 호르몬 수용체 활성을 나타내는 글루카곤 슈퍼패밀리 펩티드
US8980249B2 (en) 2010-06-03 2015-03-17 University Of Miami Agonists of growth hormone releasing hormone as effectors for survival and proliferation of pancreatic islets
EP2582715B1 (en) 2010-06-16 2018-11-28 The Administrators of the Tulane Educational Fund Growth hormone secretatogue receptor antagonists and uses thereof
US20130177979A1 (en) 2010-06-22 2013-07-11 University Of Central Florida Research Foundation, Inc. Methods and compositions for cell permeable stat3 inhibitor
US20110313167A1 (en) 2010-06-22 2011-12-22 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Substituted Heterocycles as Therapeutic agents for treating cancer
US20130137849A1 (en) 2010-06-24 2013-05-30 Indiana University Research And Technology Corporation Dipeptide linked medicinal agents
RU2580317C2 (ru) 2010-06-24 2016-04-10 Индиана Юниверсити Рисерч Энд Текнолоджи Корпорейшн Пептидные пролекарства, принадлежащие к суперсемейству амид-содержащих глюкагонов
US8907053B2 (en) 2010-06-25 2014-12-09 Aurigene Discovery Technologies Limited Immunosuppression modulating compounds
WO2012012352A2 (en) 2010-07-19 2012-01-26 Amidebio, Llc Modified peptides and proteins
US8975232B2 (en) 2010-07-29 2015-03-10 President And Fellows Of Harvard College Macrocyclic kinase inhibitors and uses thereof
CA2807685C (en) 2010-08-13 2020-10-06 Aileron Therapeutics, Inc. P53 derived peptidomimetic macrocycle
JP2013537191A (ja) 2010-09-08 2013-09-30 ユニバーシティー オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション ｐ５３−Ｍｄｍ２アンタゴニスト
US20120065210A1 (en) 2010-09-15 2012-03-15 Xin-Jie Chu Substituted hexahydropyrrolo[1,2-c]imidazolones
EP2616095A4 (en) 2010-09-16 2014-03-19 Univ Miami ACCELERATING WOUND HEALING WITH HORMONE FOR RELEASE OF GROWTH HORMONE AND AGONISTS
EP2431035A1 (en) 2010-09-16 2012-03-21 Æterna Zentaris GmbH Novel Triazole Derivatives with Improved Receptor Activity and Bioavailability Properties as Ghrelin Antagonists of Growth Hormone Secretagogue Receptors
CN102399284B (zh) 2010-09-17 2013-05-29 中国农业大学 狐狸生长激素释放激素cDNA及其应用
CN102399283B (zh) 2010-09-17 2013-05-29 中国农业大学 水貂生长激素释放激素cDNA及其应用
US20120071499A1 (en) 2010-09-20 2012-03-22 Xin-Jie Chu Substituted Spiro[3H-Indole-3,6'(5'H)-[1H]Pyrrolo[1,2c]Imidazole-1',2(1H,2'H)-diones
WO2012040459A2 (en) 2010-09-22 2012-03-29 President And Fellows Of Harvard College Beta-catenin targeting peptides and uses thereof
US20130225603A1 (en) 2010-09-27 2013-08-29 Serrata Llc Mdm2 inhibitors for treatment of ocular conditions
US9266860B2 (en) 2010-09-30 2016-02-23 St. Jude Children's Research Hospital Aryl-substituted imidazoles
US9169295B2 (en) 2010-10-13 2015-10-27 Bristol-Myers Squibb Company Macrocycles and macrocycle stabilized peptides
FR2967072B1 (fr) 2010-11-05 2013-03-29 Univ Dundee Procede pour ameliorer la production de virus et semences vaccinales influenza
CA2817568A1 (en) 2010-11-12 2012-05-18 The Salk Institute For Biological Studies Intellectual Property And Tech Nology Transfer Cancer therapies and diagnostics
CA2817585A1 (en) 2010-11-12 2012-05-18 The Regents Of The University Of Michigan Spiro-oxindole mdm2 antagonists
WO2012076513A1 (en) 2010-12-09 2012-06-14 F. Hoffmann-La Roche Ag 3-cyano-1-hydroxymethyl-2-phenylpyrrolidine derivatives as inhibitors of mdm2-p53 interactions useful for the treatment of cancer
WO2012083078A2 (en) 2010-12-15 2012-06-21 The Research Foundation Of State University Of New York Croos-linked peptides and proteins, methods of making same, and uses thereof
BR112013014522A2 (pt) 2010-12-16 2017-09-26 Roche Glycart Ag anticorpo anti-cd20 afucosilado e seu uso, composição e método para tratamento de um paciente que sofre de câncer
WO2012083181A1 (en) 2010-12-16 2012-06-21 Indiana University Research And Technology Corporation Alpha helix mimetics and methods for using
US20130253011A1 (en) 2010-12-17 2013-09-26 Syngenta Participations Ag Insecticidal compounds
EP2474625B1 (en) 2011-01-05 2016-11-02 Daniela Kandioler Method for determining the p53 status of a tumour
EP2474624B1 (en) 2011-01-05 2016-08-17 Daniela Kandioler Response prediction in cancer treatment (p53 adapted cancer therapy)
US20130302909A1 (en) 2011-01-14 2013-11-14 Theratechnologies Inc. Assessment of igf-1 levels in hiv-infected subjects and uses thereof
JP6239979B2 (ja) 2011-03-04 2017-11-29 ニューヨーク・ユニバーシティ Ｒａｓの調節因子としての水素結合代替大環状分子
CN103415530A (zh) 2011-03-09 2013-11-27 Jitsubo株式会社 新型的含有非肽性交联结构的交联肽、以及该交联肽的合成方法和用于该方法的新型有机化合物
HUE038714T2 (hu) 2011-03-10 2018-11-28 Daiichi Sankyo Co Ltd Dispiropirrolidin származék
CA2834657A1 (en) 2011-04-29 2012-11-01 Kinemed, Inc. Chemical modification of apolipoprotein mimetic peptides for the production of therapeutic agents
WO2012173846A2 (en) 2011-06-06 2012-12-20 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
WO2012174423A1 (en) 2011-06-17 2012-12-20 President And Fellows Of Harvard College Stabilized polypeptides as regulators of rab gtpase function
JP2014520120A (ja) 2011-06-17 2014-08-21 プレジデント アンド フェローズ オブ ハーバード カレッジ 安定化した変異型ｍａｍｌペプチドおよびその使用
GB201110390D0 (en) 2011-06-20 2011-08-03 Medical Res Council Compounds for use in stabilising p53 mutants
US20120328692A1 (en) 2011-06-24 2012-12-27 University Of Maryland, Baltimore Potent d-peptide antagonists of mdm2 and mdmx for anticancer therapy
CN103889437A (zh) 2011-08-31 2014-06-25 纽约大学 硫醚、醚和烷基胺连接的氢键替代物肽模拟物
EP2753635A4 (en) 2011-09-09 2015-08-12 Agency Science Tech & Res PEPTIDES THAT ACTIVATE P53 PROTEIN
WO2013049250A1 (en) 2011-09-27 2013-04-04 Amgen Inc. Heterocyclic compounds as mdm2 inhibitors for the treatment of cancer
WO2013059530A2 (en) 2011-10-18 2013-04-25 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
US9096684B2 (en) 2011-10-18 2015-08-04 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
EP2771008A4 (en) 2011-10-28 2015-04-08 Merck Sharp & Dohme MACROCYCLES INCREASING P53 ACTIVITY AND USES THEREOF
US9408885B2 (en) 2011-12-01 2016-08-09 Vib Vzw Combinations of therapeutic agents for treating melanoma
CA2761253A1 (en) 2011-12-07 2013-06-07 Vib Vzw Combinations of therapeutic agents for treating melanoma
WO2013123266A1 (en) 2012-02-15 2013-08-22 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
JP6450192B2 (ja) 2012-02-15 2019-01-09 エイルロン セラピューティクス，インコーポレイテッド トリアゾール架橋した、およびチオエーテル架橋したペプチドミメティック大環状化合物
US9880171B2 (en) 2012-03-02 2018-01-30 Ludwig Institute For Cancer Research Ltd. iASPP phosphorylation and metastatic potential
US9216170B2 (en) 2012-03-19 2015-12-22 Hoffmann-La Roche Inc. Combination therapy for proliferative disorders
CA3113959A1 (en) 2012-09-26 2014-04-03 President And Fellows Of Harvard College Proline-locked stapled peptides and uses thereof
US20150225471A1 (en) 2012-10-01 2015-08-13 President And Fellows Of Harvard College Stabilized polypeptide insulin receptor modulators
US9604919B2 (en) 2012-11-01 2017-03-28 Aileron Therapeutics, Inc. Disubstituted amino acids and methods of preparation and use thereof
WO2014138429A2 (en) 2013-03-06 2014-09-12 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and use thereof in regulating hif1alpha
CN110105434A (zh) 2013-03-13 2019-08-09 哈佛大学的校长及成员们 钉合且缝合的多肽及其用途
US9198910B2 (en) 2013-04-04 2015-12-01 The Translational Genomics Research Institute Methods for the treatment of cancer
JP2016523241A (ja) 2013-06-14 2016-08-08 プレジデント アンド フェローズ オブ ハーバード カレッジ 安定化されたポリペプチドインスリン受容体調節剤
US20160022720A1 (en) 2013-08-02 2016-01-28 Children's Hospital Medical Center Compositions and methods for treating disease states associated with activated t cells and/or b cells
JP2017503749A (ja) 2013-10-01 2017-02-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 安定化されたポリペプチドおよびその使用
LT3068393T (lt) 2013-11-11 2022-08-10 Amgen Inc. Kombinuota terapija, įskaitant mdm2 inhibitorių ir vieną arba daugiau papildomų farmaciškai aktyvių agentų, skirta vėžiui gydyti
SG11201604504VA (en) 2013-12-05 2016-07-28 Hoffmann La Roche Novel combination treatment for acute myeloid leukemia (aml)
RU2016129953A (ru) 2013-12-23 2018-01-30 Новартис Аг Фармацевтические комбинации
WO2015157508A1 (en) 2014-04-09 2015-10-15 Aileron Therapeutics, Inc. Peptidomimetic macrocycles with pth activity
TW201613576A (en) 2014-06-26 2016-04-16 Novartis Ag Intermittent dosing of MDM2 inhibitor
SG11201702175YA (en) 2014-09-24 2017-04-27 Aileron Therapeutics Inc Peptidomimetic macrocycles and formulations thereof
AU2015320549A1 (en) 2014-09-24 2017-04-13 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
BR112017019738A2 (pt) 2015-03-20 2018-05-29 Aileron Therapeutics Inc macrociclos peptidomiméticos e usos dos mesmos
WO2017004548A1 (en) 2015-07-01 2017-01-05 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
EP3317294B1 (en) 2015-07-02 2023-03-15 Dana-Farber Cancer Institute, Inc. Stabilized anti-microbial peptides
US20170037105A1 (en) 2015-08-03 2017-02-09 Aileron Therapeutics, Inc. Peptidomimetic macrocycles
WO2017040990A1 (en) 2015-09-03 2017-03-09 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
EP3347372A4 (en) 2015-09-10 2019-09-04 Aileron Therapeutics, Inc. PEPTIDOMIMETIC MACROCYCLES AS MODULATORS OF MCL-1
EP3432904A4 (en) 2016-03-21 2020-03-11 Aileron Therapeutics, Inc. COMPANION DIAGNOSTIC TOOL FOR PEPTIDOMIMETIC MACROCYCLES
WO2017205786A1 (en) 2016-05-27 2017-11-30 Aileron Therapeutics, Inc. Cell permeable peptidomimetic macrocycles
US20170360881A1 (en) 2016-06-17 2017-12-21 Aileron Therapeutics, Inc. Peptidomimetic macrocycles and uses thereof
WO2018165575A2 (en) 2017-03-09 2018-09-13 Aileron Therapeutics, Inc. Warhead-containing peptidomimetic macrocycles as modulators of bfl-1

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137974A2 (en) * 2005-06-13 2006-12-28 Tranzyme Pharma, Inc. Macrocyclic ghrelin receptor antagonists and inverse agonists and methods of using the same
WO2009126292A2 (en) * 2008-04-08 2009-10-15 Aileron Therapeutics, Inc. Biologically active peptidomimetic macrocycles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Hu B. et al, Cancer Res 67(18):pp.8810-8817(2007. 9.15.).* *

Also Published As

Publication number Publication date
US20140378390A1 (en) 2014-12-25
CN103282510A (zh) 2013-09-04
PT2603600T (pt) 2019-02-27
US11008366B2 (en) 2021-05-18
DK2603600T3 (da) 2019-03-04
KR20130099938A (ko) 2013-09-06
JP2017019804A (ja) 2017-01-26
JP2019031494A (ja) 2019-02-28
ES2711526T3 (es) 2019-05-06
JP6643958B2 (ja) 2020-02-12
US20200102351A1 (en) 2020-04-02
EP2603600A4 (en) 2014-10-29
RU2013110845A (ru) 2014-09-20
WO2012021875A1 (en) 2012-02-16
US20160257716A1 (en) 2016-09-08
EP2603600B1 (en) 2018-11-21
RU2582678C2 (ru) 2016-04-27
JP2013535514A (ja) 2013-09-12
MX2013001748A (es) 2013-06-05
KR102104762B1 (ko) 2020-04-24
MX355543B (es) 2018-04-20
US20190062377A1 (en) 2019-02-28
WO2012021876A2 (en) 2012-02-16
SI2603600T1 (sl) 2019-04-30
RU2016111360A (ru) 2018-11-27
US20130210743A1 (en) 2013-08-15
BR112013003248A2 (pt) 2016-06-07
KR20180058866A (ko) 2018-06-01
EP2603600A2 (en) 2013-06-19
US9957299B2 (en) 2018-05-01
KR20170058446A (ko) 2017-05-26
WO2012021874A1 (en) 2012-02-16
US8859723B2 (en) 2014-10-14
US10703780B2 (en) 2020-07-07
CA2807685A1 (en) 2012-02-16
CN108570097A (zh) 2018-09-25
CA2807685C (en) 2020-10-06
PL2603600T3 (pl) 2019-06-28

Similar Documents

Publication Publication Date Title
KR102104762B1 (ko) 펩티도미메틱 거대고리
JP6694459B2 (ja) ペプチドミメティック大環状化合物
US10227380B2 (en) Triazole-crosslinked and thioether-crosslinked peptidomimetic macrocycles
JP2012510430A (ja) ペプチド模倣大環状分子
JP2013505300A (ja) ペプチド模倣大環状分子
JP2013507927A (ja) 改善されたペプチド模倣大環状分子

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant