JP6367196B2 - マイクロカプセル組成物および方法 - Google Patents

マイクロカプセル組成物および方法 Download PDF

Info

Publication number
JP6367196B2
JP6367196B2 JP2015527549A JP2015527549A JP6367196B2 JP 6367196 B2 JP6367196 B2 JP 6367196B2 JP 2015527549 A JP2015527549 A JP 2015527549A JP 2015527549 A JP2015527549 A JP 2015527549A JP 6367196 B2 JP6367196 B2 JP 6367196B2
Authority
JP
Japan
Prior art keywords
dna
microcapsules
chemical
cases
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015527549A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015528283A (ja
Inventor
ベンジャミン・ハインドソン
セルジュ・サクソノフ
Original Assignee
テンエックス・ジェノミクス・インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50101458&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP6367196(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by テンエックス・ジェノミクス・インコーポレイテッド filed Critical テンエックス・ジェノミクス・インコーポレイテッド
Publication of JP2015528283A publication Critical patent/JP2015528283A/ja
Application granted granted Critical
Publication of JP6367196B2 publication Critical patent/JP6367196B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015527549A 2012-08-14 2013-08-13 マイクロカプセル組成物および方法 Active JP6367196B2 (ja)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201261683192P 2012-08-14 2012-08-14
US61/683,192 2012-08-14
US201261737374P 2012-12-14 2012-12-14
US61/737,374 2012-12-14
US201361762435P 2013-02-08 2013-02-08
US61/762,435 2013-02-08
US201361800223P 2013-03-15 2013-03-15
US61/800,223 2013-03-15
US201361840403P 2013-06-27 2013-06-27
US61/840,403 2013-06-27
US201361844804P 2013-07-10 2013-07-10
US61/844,804 2013-07-10
PCT/US2013/054797 WO2014028537A1 (en) 2012-08-14 2013-08-13 Microcapsule compositions and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018127432A Division JP2018157832A (ja) 2012-08-14 2018-07-04 マイクロカプセル組成物および方法

Publications (2)

Publication Number Publication Date
JP2015528283A JP2015528283A (ja) 2015-09-28
JP6367196B2 true JP6367196B2 (ja) 2018-08-01

Family

ID=50101458

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015527549A Active JP6367196B2 (ja) 2012-08-14 2013-08-13 マイクロカプセル組成物および方法
JP2018127432A Pending JP2018157832A (ja) 2012-08-14 2018-07-04 マイクロカプセル組成物および方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2018127432A Pending JP2018157832A (ja) 2012-08-14 2018-07-04 マイクロカプセル組成物および方法

Country Status (12)

Country Link
US (13) US20140155295A1 (enExample)
EP (3) EP2885418A4 (enExample)
JP (2) JP6367196B2 (enExample)
KR (1) KR102090851B1 (enExample)
CN (4) CN104769127A (enExample)
AU (5) AU2013302756C1 (enExample)
BR (1) BR112015003354A8 (enExample)
CA (2) CA3216609C (enExample)
IL (1) IL237156B (enExample)
IN (1) IN2015DN01126A (enExample)
MX (1) MX364957B (enExample)
WO (1) WO2014028537A1 (enExample)

Families Citing this family (394)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
BRPI1008965B1 (pt) 2009-03-13 2018-12-18 Harvard College método para aumento de escala de dispositivos microfluídicos e sistema para a formação de gotículas em canais microfluídicos em paralelo
US10688026B2 (en) 2009-04-27 2020-06-23 Premier Dental Products Company Buffered microencapsulated compositions and methods
US9814657B2 (en) 2009-04-27 2017-11-14 Premier Dental Products Company Buffered microencapsulated compositions and methods
WO2011028764A2 (en) 2009-09-02 2011-03-10 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
EP3461558B1 (en) 2009-10-27 2021-03-17 President and Fellows of Harvard College Droplet creation techniques
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3789498B1 (en) 2011-04-25 2025-08-13 Bio-Rad Laboratories, Inc. Methods for nucleic acid analysis
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
WO2013019491A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
JP6375230B2 (ja) 2012-02-27 2018-08-15 セルラー リサーチ， インコーポレイテッド 分子計数のための組成物およびキット
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
EP3305918B1 (en) 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
EP4001426A1 (en) 2012-08-13 2022-05-25 The Regents of The University of California Methods and systems for detecting biological components
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2885418A4 (en) 2012-08-14 2016-03-02 10X Genomics Inc MICROCAPSE COMPOSITIONS AND METHOD THEREFOR
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
AU2014302277A1 (en) 2013-06-27 2015-12-24 10X Genomics, Inc. Compositions and methods for sample processing
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN111394444A (zh) 2013-09-13 2020-07-10 生命技术公司 使用凝聚核酸颗粒的装置制备
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
US10947587B2 (en) * 2013-11-05 2021-03-16 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
EP3065712A4 (en) 2013-11-08 2017-06-21 President and Fellows of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
KR102596508B1 (ko) * 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
US10518241B2 (en) * 2014-04-15 2019-12-31 Agilent Technologies, Inc. Creating and harvesting surface-bound emulsion
EP3132072B1 (en) * 2014-04-17 2019-12-04 President and Fellows of Harvard College Methods for droplet tagging
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN114214314A (zh) * 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CA2953469A1 (en) * 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3161700B1 (en) 2014-06-26 2023-03-29 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
US20150376605A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods and Compositions for Sample Analysis
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
WO2016004234A1 (en) * 2014-07-02 2016-01-07 Life Technologies Corporation Methods for loading a sensor substrate
EP3191605B1 (en) 2014-09-09 2022-07-27 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
WO2016065056A1 (en) 2014-10-22 2016-04-28 The Regents Of The University Of California High definition microdroplet printer
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
EP4092681A1 (en) 2015-01-13 2022-11-23 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
FR3031914B1 (fr) * 2015-01-27 2019-06-07 Calyxia Procede d'encapsulation
CN115011670A (zh) 2015-02-04 2022-09-06 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN107208156B (zh) 2015-02-09 2021-10-08 10X基因组学有限公司 用于使用变异识别数据来确定结构变异和定相的系统和方法
CN107250379B (zh) 2015-02-19 2021-12-28 贝克顿迪金森公司 结合蛋白质组信息和基因组信息的高通量单细胞分析
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
CN107614700A (zh) 2015-03-11 2018-01-19 布罗德研究所有限公司 基因型和表型偶联
EP3929291A1 (en) 2015-03-17 2021-12-29 Bio-Rad Laboratories, Inc. Detection of genome editing
WO2016149661A1 (en) 2015-03-18 2016-09-22 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
JP2018511341A (ja) 2015-04-17 2018-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子配列決定および他の適用のためのバーコード化システムおよび方法
CN107810277B (zh) 2015-04-21 2021-11-02 通用自动化实验技术公司 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
US10712356B2 (en) 2015-04-21 2020-07-14 General Automation Lab Technologies Inc. Apparatus and method for picking biological sample
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
WO2016183029A1 (en) 2015-05-11 2016-11-17 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
US9771575B2 (en) 2015-06-19 2017-09-26 Agilent Technologies, Inc. Methods for on-array fragmentation and barcoding of DNA samples
US10501777B2 (en) 2015-07-17 2019-12-10 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
US10640816B2 (en) 2015-07-17 2020-05-05 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
US10189718B1 (en) * 2015-09-01 2019-01-29 Hrl Laboratories, Llc Methods for charge-titrating particle assembly, and structures produced therefrom
US10974959B1 (en) * 2015-09-01 2021-04-13 Hrl Laboratories, Llc Methods for charge-titrating assembly of partially metallized nanoparticles, and metamaterials produced therefrom
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
CN106552296B (zh) * 2015-09-29 2020-08-14 上海氪励铵勤科技发展有限公司 纳米粒子、其制备方法与结石取出装置及应用
AU2016338907B2 (en) 2015-10-13 2022-07-07 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN108368522A (zh) 2015-10-20 2018-08-03 10X 基因组学有限公司 用于高通量单细胞遗传操作的方法和系统
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
DE102015221850A1 (de) * 2015-11-06 2017-05-11 Carl Zeiss Ag Verfahren zur Präparation von Referenzmarkierungen auf einem Probenträger
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
CN110343753B (zh) * 2015-11-19 2022-06-21 赛纳生物科技(北京)有限公司 一种磷酸修饰荧光团的核苷酸分子测序方法
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
US12071663B2 (en) 2016-01-15 2024-08-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017165620A1 (en) * 2016-03-24 2017-09-28 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
CN110199019B (zh) 2016-05-02 2024-09-10 Encodia有限公司 采用核酸编码的大分子分析
WO2017192387A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
JP2017203665A (ja) * 2016-05-10 2017-11-16 国立大学法人信州大学 光開裂性マイクロカプセル、それを用いたセンサ、及びそれを用いた被測定物質の測定方法
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
CN110088290A (zh) 2016-08-10 2019-08-02 加利福尼亚大学董事会 在乳液微滴中结合多重置换扩增和pcr
US11219919B1 (en) 2016-08-19 2022-01-11 Hrl Laboratories, Llc Electrostatically driven assembly of nanoparticle materials into dense films
WO2018039338A1 (en) 2016-08-23 2018-03-01 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
BR112019005900A2 (pt) 2016-09-26 2019-06-11 Cellular Research, Inc. medição de expressão de proteínas usando reagentes com sequências de oligonucleotídeos com código de barras
EP4026905B1 (en) 2016-10-19 2024-04-17 10X Genomics, Inc. Methods for barcoding nucleic acid molecules from individual cells or cell populations
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
SG11201903139SA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for expression profile classification
US11608497B2 (en) 2016-11-08 2023-03-21 Becton, Dickinson And Company Methods for cell label classification
CN118441026A (zh) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
EP3571308A4 (en) 2016-12-21 2020-08-19 The Regents of The University of California GENOMIC SEQUENCING OF SINGLE CELLS USING HYDROGEL-BASED DROPS
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4628582A2 (en) 2017-01-30 2025-10-08 10x Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3838268B1 (en) * 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
EP3601595A4 (en) 2017-03-21 2020-12-16 Muwells, Inc. SEALED MICROWELL DOSAGE
JP6911466B2 (ja) * 2017-03-31 2021-07-28 ソニーグループ株式会社 細胞の代謝を測定するためのマイクロチップ、細胞の代謝を測定するための装置、細胞の代謝を測定する方法及び細胞の代謝を測定するためのシステム
SG11201908596WA (en) 2017-04-26 2019-11-28 10X Genomics Inc Mmlv reverse transcriptase variants
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
JP7197567B2 (ja) * 2017-05-05 2022-12-27 スキピオ バイオサイエンス ハイドロゲル中の個別の生物学的単位を捕捉およびバーコード付与するための方法
EP4215616B1 (en) 2017-05-18 2024-09-04 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3403724A1 (en) * 2017-05-18 2018-11-21 Hifibio Process for manufacturing an array with microchannels
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. DATA SET ANALYSIS SYSTEMS AND METHODS
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2018236615A1 (en) 2017-06-20 2018-12-27 10X Genomics, Inc. Methods and systems for improved droplet stabilization
US10498001B2 (en) 2017-08-21 2019-12-03 Texas Instruments Incorporated Launch structures for a hermetically sealed cavity
US10821442B2 (en) 2017-08-22 2020-11-03 10X Genomics, Inc. Devices, systems, and kits for forming droplets
US10391493B2 (en) 2017-08-29 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US10748643B2 (en) 2017-08-31 2020-08-18 10X Genomics, Inc. Systems and methods for determining the integrity of test strings with respect to a ground truth string
US10775422B2 (en) 2017-09-05 2020-09-15 Texas Instruments Incorporated Molecular spectroscopy cell with resonant cavity
US10589986B2 (en) 2017-09-06 2020-03-17 Texas Instruments Incorporated Packaging a sealed cavity in an electronic device
US10444102B2 (en) 2017-09-07 2019-10-15 Texas Instruments Incorporated Pressure measurement based on electromagnetic signal output of a cavity
US10551265B2 (en) 2017-09-07 2020-02-04 Texas Instruments Incorporated Pressure sensing using quantum molecular rotational state transitions
US10549986B2 (en) 2017-09-07 2020-02-04 Texas Instruments Incorporated Hermetically sealed molecular spectroscopy cell
US10131115B1 (en) 2017-09-07 2018-11-20 Texas Instruments Incorporated Hermetically sealed molecular spectroscopy cell with dual wafer bonding
US10424523B2 (en) 2017-09-07 2019-09-24 Texas Instruments Incorporated Hermetically sealed molecular spectroscopy cell with buried ground plane
US10544039B2 (en) * 2017-09-08 2020-01-28 Texas Instruments Incorporated Methods for depositing a measured amount of a species in a sealed cavity
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019071039A1 (en) 2017-10-04 2019-04-11 10X Genomics, Inc. COMPOSITIONS, METHODS AND SYSTEMS FOR PEARL FORMATION USING ENHANCED POLYMERS
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084328A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS FOR PREPARING NUCLEIC ACID MOLECULES
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
EP4241882B1 (en) 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
WO2019089836A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
EP3704247B1 (en) 2017-11-02 2023-01-04 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
CN117604074A (zh) 2017-11-17 2024-02-27 10X基因组学有限公司 用于关联生物颗粒的物理和遗传特性的方法和系统
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
EP3919626B1 (en) 2017-12-08 2024-07-10 10X Genomics, Inc. Methods and compositions for labeling cells
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
WO2019126466A1 (en) * 2017-12-22 2019-06-27 Rootpath Genomics, Inc. Compositions and methods for barcoding
EP3505933A1 (en) * 2017-12-29 2019-07-03 Blink AG A microcapsule for detecting and/or quantitating an analyte in a sample
WO2019148042A1 (en) 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
US12209277B2 (en) 2018-01-31 2025-01-28 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US12377396B2 (en) 2018-02-05 2025-08-05 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
US20190249248A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112074610A (zh) 2018-02-22 2020-12-11 10X基因组学有限公司 接合介导的核酸分析
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019177927A1 (en) * 2018-03-14 2019-09-19 Premier Dental Products Company Buffered microencapsulated compositions and methods
WO2019191321A1 (en) 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11318469B2 (en) 2018-04-25 2022-05-03 Hewlett-Packard Development Company, L.P. Selective release of material in thermally degradable capsule
US11577252B2 (en) 2018-04-27 2023-02-14 Hewlett-Packard Development Company, L.P. Thermally controlled reagent release
WO2019212536A1 (en) 2018-05-01 2019-11-07 Hewlett-Packard Development Company, L.P. Sequential encapsulation of reagents
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
CN112243461B (zh) 2018-05-03 2024-07-12 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2019219757A1 (de) * 2018-05-15 2019-11-21 Albert-Ludwig-Universität Freiburg Mikroarray-transformer
WO2019226631A1 (en) * 2018-05-21 2019-11-28 The Regents Of The University Of California Single cell mapping and transcriptome analysis
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
CN112368562A (zh) * 2018-07-11 2021-02-12 香港大学 用于液滴的连续和定量收集的自动微流控系统
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
CN116144706A (zh) 2018-08-13 2023-05-23 根路径基因组学公司 成对的二分免疫受体多核苷酸的高通量克隆及其应用
CA3109426A1 (en) * 2018-08-17 2020-02-20 The Regents Of The University Of California Particle-containing droplet systems with monodisperse fluid volumes
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US20210332425A1 (en) 2018-08-28 2021-10-28 Rajiv Bharadwaj Increasing spatial array resolution
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN112805389B (zh) 2018-10-01 2024-08-06 贝克顿迪金森公司 确定5’转录物序列
FR3086951B1 (fr) * 2018-10-05 2021-02-26 Redberry Methode et dispositif pour la detection d'au moins un microorganisme selon sa cinetique de marquage, et support de detection
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US20220033888A1 (en) 2018-12-10 2022-02-03 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020123657A2 (en) 2018-12-11 2020-06-18 10X Genomics, Inc. Methods and devices for detecting and sorting droplets or particles
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
DE102019130236A1 (de) * 2018-12-20 2020-06-25 Endress+Hauser Conducta Gmbh+Co. Kg Verfahren und System zum Herstellen einer Lösung
WO2020139844A1 (en) 2018-12-24 2020-07-02 10X Genomics, Inc. Devices, systems, and methods for controlling liquid flow
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3906318A1 (en) 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US20200232979A1 (en) * 2019-01-22 2020-07-23 Mayo Foundation For Medical Education And Research Microcapsules and Methods for Analyte Detection
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167656A1 (en) 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
CA3127279A1 (en) 2019-02-12 2020-08-20 Therycell Gmbh Reverse immunosuppression
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020176788A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3930900A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
EP3938537A1 (en) 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
US11976269B2 (en) * 2019-03-18 2024-05-07 Cellular Research, Inc. Precise delivery of components into fluids
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
EP3947530A1 (en) 2019-04-03 2022-02-09 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
JP2022527364A (ja) 2019-04-05 2022-06-01 ルートパス・ジェノミクス，インコーポレーテッド Ｔ細胞受容体遺伝子アセンブリのための組成物及び方法
US12436151B2 (en) 2019-04-12 2025-10-07 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymmatically releasable detection moiety and a barcode moiety
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020218551A1 (ja) * 2019-04-26 2020-10-29 ｂｉｔＢｉｏｍｅ株式会社 ゲルカプセル方式による１細胞ゲノムライブラリーからの配列スクリーニング法
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
US11578322B2 (en) 2019-05-07 2023-02-14 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
AU2020280104A1 (en) 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3982716A4 (en) 2019-06-14 2023-09-06 Bio-Rad Laboratories, Inc. System and method for automated single cell processing and analyses
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
EP4025709A1 (en) 2019-09-06 2022-07-13 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
EP4194855A1 (en) 2019-10-11 2023-06-14 10X Genomics, Inc. Methods for analyte detection and analysis
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
ES2987640T3 (es) 2019-11-08 2024-11-15 Becton Dickinson Co Uso de cebado aleatorio para obtener información de V(D)J de longitud completa para la secuenciación del repertorio inmunitario
US20220364147A1 (en) 2019-11-15 2022-11-17 Miltenyi Biotec B.V. & Co. KG Color and bardcoded beads for single cell indexing
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
EP4065280A1 (en) 2019-11-27 2022-10-05 10X Genomics, Inc. Devices, systems, and methods for generating droplets
EP4073573A4 (en) 2019-12-10 2024-04-10 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
EP4461810A3 (en) 2019-12-11 2025-04-09 10X Genomics, Inc. Reverse transcriptase variants
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US11104961B2 (en) 2020-01-13 2021-08-31 Fluent Biosciences Inc. Single cell sequencing
US20210214769A1 (en) * 2020-01-13 2021-07-15 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
CN115768558A (zh) 2020-01-13 2023-03-07 福路伦特生物科学公司 用于单细胞基因谱分析的方法和系统
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
CN115335520A (zh) 2020-01-29 2022-11-11 贝克顿迪金森公司 用于通过测序对单细胞进行空间映射的条形码化的孔
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
ES2980267T3 (es) 2020-02-10 2024-09-30 Hoffmann La Roche Sistema de distribución de líquido para un portador de muestras de microfluidos, sistema de sellado para portador de muestras de microfluidos que incluye dicho sistema de distribución de líquido y procedimiento para distribuir líquido de sellado usando el mismo
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
WO2021163611A1 (en) 2020-02-13 2021-08-19 10X Genomics, Inc. Methods for characterizing cells using gene expression and chromatin accessibility
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
WO2021174051A1 (en) 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4121528A1 (en) 2020-03-17 2023-01-25 F. Hoffmann-La Roche AG Method of improving cell recovery in single-cell analysis
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN115916972A (zh) 2020-04-16 2023-04-04 10X基因组学有限公司 用于被固定样品的组合物和方法
WO2021222301A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for analysis and identification of barcode multiplets
WO2021222302A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for increasing cell recovery efficiency
WO2021226290A1 (en) 2020-05-05 2021-11-11 10X Genomics, Inc. Methods for identification of antigen-binding molecules
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4407030B1 (en) 2020-06-02 2025-12-17 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
EP4158058B1 (en) 2020-06-02 2025-08-06 10X Genomics, Inc. Enrichment of nucleic acid sequences
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
EP4121557A4 (en) 2020-08-06 2024-05-01 Singular Genomics Systems, Inc. Spatial sequencing
CN116171200A (zh) 2020-09-02 2023-05-26 10X基因组学有限公司 用于高通量液滴形成的装置、系统和方法
EP4208292B1 (en) 2020-09-02 2024-11-06 10X Genomics, Inc. Flow focusing devices, systems, and methods for high throughput droplet formation
CN115997126B (zh) 2020-09-07 2025-11-28 美天施生物科技有限两合公司 具有可酶促释放的检测部分和条形码部分的缀合物
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
EP4410956A3 (en) 2020-09-23 2025-04-09 10X Genomics, Inc. Selective enzymatic gelation
WO2022076914A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for profiling immune repertoire
WO2022076912A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for analyzing antigen binding molecules
US12298314B2 (en) 2020-10-09 2025-05-13 10X Genomics, Inc. Methods and compositions for analyzing target binding of molecules
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
US12436088B2 (en) 2020-11-03 2025-10-07 Droplet Genomics, Uab Integrated platform for selective microfluidic particle processing
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4001432A1 (en) 2020-11-13 2022-05-25 Miltenyi Biotec B.V. & Co. KG Algorithmic method for efficient indexing of genetic sequences using associative arrays
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
EP4275051A1 (en) 2021-01-08 2023-11-15 10X Genomics, Inc. Methods for generating antigen-binding molecules from single cells
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
US12390775B1 (en) 2021-02-08 2025-08-19 10X Genomics, Inc. Devices and methods for reducing the effects of settling of particles during droplet production
EP4294571B8 (en) 2021-02-19 2024-07-10 10X Genomics, Inc. Method of using a modular assay support device
WO2022178304A1 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. High-throughput methods for analyzing and affinity-maturing an antigen-binding molecule
EP4298239B1 (en) 2021-02-23 2025-11-05 10X Genomics, Inc. Drug screening methods
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022182662A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Compositions and methods for mapping antigen-binding molecule affinity to antigen regions of interest
WO2022182664A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. A method for epitope binning of novel monoclonal antibodies
WO2022182672A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Single cell glycan profiling
WO2022182865A1 (en) 2021-02-24 2022-09-01 10X Genomics, Inc. Method for concentrating droplets in an emulsion
CN117241878A (zh) 2021-03-05 2023-12-15 伊努梅里斯公司 用于生成微滴和执行数字分析的系统和方法
CN117098606A (zh) 2021-03-26 2023-11-21 10X基因组学有限公司 用于改进液滴回收的装置、方法和系统
WO2022221428A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Compositions and methods for single cell analyte detection and analysis
IL307619A (en) * 2021-04-16 2023-12-01 Plexium Inc Covers for testing devices
JP2024516375A (ja) * 2021-04-16 2024-04-15 プレクシアム・インコーポレイテッド 組合せライブラリ用分析装置
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
CA3220479A1 (en) * 2021-05-18 2022-11-24 Fluent Biosciences Inc. Template particles with micropores and nanopores
WO2022256313A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Validation of a unique molecular identifier associated with a nucleic acid sequence of interest
WO2022256345A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics
EP4530361A3 (en) 2021-07-26 2025-07-02 10x Genomics, Inc. Nucleic acid processing via circularization
CN113736850A (zh) * 2021-08-13 2021-12-03 纳昂达(南京)生物科技有限公司 基于双链环化的文库构建方法及其在测序中的应用
WO2023022925A1 (en) 2021-08-17 2023-02-23 10X Genomics, Inc. Compositions, systems and methods for enzyme optimization
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
WO2023060110A1 (en) 2021-10-05 2023-04-13 10X Genomics, Inc. Methods of immune cell analysis
WO2023076832A1 (en) 2021-10-25 2023-05-04 Singular Genomics Systems, Inc. Manipulating and detecting biological samples
CN118369438A (zh) 2021-12-10 2024-07-19 伯乐实验室有限公司 用形态可调节功能化颗粒进行样品处理的组合物、方法和系统
WO2023114203A1 (en) 2021-12-13 2023-06-22 Cornell University Genotyping of targeted loci with single-cell chromatin accessibility
WO2023114310A1 (en) 2021-12-15 2023-06-22 10X Genomics, Inc. Methods for improving sensitivity of immune profiling using oligo-tagged antigens
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
EP4453246A1 (en) 2021-12-20 2024-10-30 Enumerix, Inc. Detection and digital quantitation of multiple targets
WO2023168423A1 (en) 2022-03-04 2023-09-07 10X Genomics, Inc. Droplet forming devices and methods having fluoropolymer silane coating agents
WO2023215320A1 (en) * 2022-05-02 2023-11-09 The Brigham And Women’S Hospital, Inc. Microfluidic platform for the study of molecular and/or cellular interactions
WO2023215861A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Reagents for characterizing antigen-binding molecules from immune cells
WO2023225201A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing t cell, or t cell-like, receptors from single cells
WO2023225259A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing antigen binding molecules from single cells
WO2023225294A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Improved major histocompatibility complex molecules
WO2023235685A1 (en) * 2022-06-01 2023-12-07 Bio-Rad Laboratories, Inc. Method and system for detecting reverse transcriptase activity by digital assay
WO2023235570A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Methods and compositions for the identification of antigen binding molecules using lipoparticle-based antigen mapping
WO2023235596A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Systems and methods for determining antigen binding specificity of antigen binding molecules
WO2023240213A2 (en) * 2022-06-10 2023-12-14 Ohio State Innovation Foundation Sustained adjunct therapy to improve chemotherapy efficacy in glioblastoma in a cerebrovascular-tumor-on-a-chip model
WO2023250422A1 (en) 2022-06-23 2023-12-28 10X Genomics, Inc. Compositions and methods for characterizing multispecific antigen binding molecules from single cells
WO2024006734A1 (en) 2022-06-27 2024-01-04 10X Genomics, Inc. Methods for preparing and using mhc multimer reagents compositions
WO2024006392A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2024015378A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods and systems for characterizing antigen-binding molecules expressed by immune cells
WO2024015733A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Improved methods and systems for identification and characterization of antigen-binding molecules from single cells
WO2024015856A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Compositions and methods for characterizing binding characteristics of antigen binding molecules from single cells
CN118201982A (zh) 2022-08-18 2024-06-14 10X基因组学有限公司 具有含氟二醇添加剂的液滴形成装置和方法
WO2024050299A1 (en) 2022-08-29 2024-03-07 10X Genomics, Inc. Improved methods and compositions for characterization of antigen-binding molecules from single cells
WO2024076908A1 (en) 2022-10-03 2024-04-11 10X Genomics, Inc. Compositions and methods for analyzing genomic insertion sites of exogenous nucleic acids
WO2024098046A1 (en) 2022-11-04 2024-05-10 10X Genomics, Inc. Systems and methods for determining antigen specificity of antigen binding molecules and visualizing adaptive immune cell clonotyping data
WO2024243444A1 (en) 2023-05-23 2024-11-28 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
CN116905093B (zh) * 2023-06-07 2024-05-24 祥符实验室 一种dna数据库的长期保存方法及其应用
WO2025064703A1 (en) 2023-09-21 2025-03-27 10X Genomics, Inc. Compositions and methods for particle hashing
WO2025096581A1 (en) 2023-10-31 2025-05-08 10X Genomics, Inc. Methods of spatial analysis and single nuclei sequencing
WO2025111369A1 (en) 2023-11-21 2025-05-30 10X Genomics, Inc. Probe-based analysis of polynucleotides and proteins
WO2025166072A1 (en) 2024-02-01 2025-08-07 10X Genomics, Inc. Compositions and methods for sample analysis
WO2025166185A1 (en) 2024-02-02 2025-08-07 10X Genomics, Inc. Methods of crispr guide rna sequencing in single cell workflows
WO2025229503A1 (en) 2024-04-29 2025-11-06 BioNTech SE Methods for identifying exhausted t cells and t-cell receptors thereof
CN119184929A (zh) * 2024-10-10 2024-12-27 上海心玮医疗科技股份有限公司 一种快速治疗颅内血管狭窄的药物支架

Family Cites Families (828)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2797149A (en) 1953-01-08 1957-06-25 Technicon International Ltd Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents
US3047367A (en) 1959-12-01 1962-07-31 Technicon Instr Automatic analysis with fluid segmentation
US3479141A (en) 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US4124638A (en) 1977-09-12 1978-11-07 Hansen John N Solubilizable polyacrylamide gels containing disulfide cross-linkages
US4253846A (en) 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
DE3230289A1 (de) 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen Herstellung von pharmazeutischen oder kosmetischen dispersionen
US4582802A (en) 1983-09-30 1986-04-15 The United States Of America As Represented By The Department Of Health And Human Services Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers
JPS60227826A (ja) * 1984-04-27 1985-11-13 Sogo Yatsukou Kk ｐＨに応答するグラフトカプセル
KR890003947B1 (ko) 1985-12-11 1989-10-13 가부시기가이샤 시마즈세이사구쇼 세포융합장치
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4872895A (en) 1986-12-11 1989-10-10 American Telephone And Telegraph Company, At&T Bell Laboratories Method for fabricating articles which include high silica glass bodies
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5185099A (en) 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
ATE176002T1 (de) 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
CA2112992C (en) 1991-07-04 2002-06-11 Allan O. F. Lihme Water-soluble, polymer-based reagents and conjugates comprising moieties derived from divinyl sulfone
CA2114950A1 (en) * 1991-08-10 1993-02-11 Michael J. Embleton Treatment of cell populations
EP0773227A1 (en) 1991-09-18 1997-05-14 Affymax Technologies N.V. Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
AU3816993A (en) 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
DE69312483T2 (de) 1992-05-01 1998-02-12 Univ Pennsylvania Mikrofabrizierte detektoren
US5840865A (en) 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
IL108497A0 (en) 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
WO1994019101A1 (en) 1993-02-16 1994-09-01 Alliance Pharmaceutical Corp. Method of microemulsifying fluorinated oils
JPH08511418A (ja) 1993-04-19 1996-12-03 メディソーブ・テクノロジーズ・インターナショナル・リミテッド・パートナーシップ 生分解性極微粒子からのアンチセンスオリゴデオキシリボヌクレオチドの徐放運搬による長期作用のある処置
US5456986A (en) 1993-06-30 1995-10-10 Carnegie Mellon University Magnetic metal or metal carbide nanoparticles and a process for forming same
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
WO1995004069A1 (en) 1993-07-30 1995-02-09 Affymax Technologies N.V. Biotinylation of proteins
AU689815B2 (en) 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US20030044777A1 (en) 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5558071A (en) 1994-03-07 1996-09-24 Combustion Electromagnetics, Inc. Ignition system power converter and controller
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
ATE190727T1 (de) 1994-05-11 2000-04-15 Genera Tech Ltd Verfahren zum einfangen von einem ligand aus einer flüssigkeit und vorrichtung zur dessen ausführung
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
WO1996029629A2 (en) 1995-03-01 1996-09-26 President And Fellows Of Harvard College Microcontact printing on surfaces and derivative articles
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
EP0832287B1 (en) 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification
CA2222581C (en) 1995-06-07 2004-05-11 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6866760B2 (en) 1998-08-27 2005-03-15 E Ink Corporation Electrophoretic medium and process for the production thereof
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US6051377A (en) 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
US5736332A (en) 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
WO1997039359A1 (en) 1996-04-15 1997-10-23 Dade International Inc. Apparatus and method for analysis
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
EP0912238B1 (en) 1996-07-15 2001-10-10 CalCiTech Ltd. Production of powders
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
AU746549B2 (en) 1996-11-20 2002-05-02 Becton Dickinson & Company Microfabricated isothermal nucleic acid amplification devices and methods
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US20020172965A1 (en) 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20050042625A1 (en) 1997-01-15 2005-02-24 Xzillion Gmbh & Co. Mass label linked hybridisation probes
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
EP1009802B1 (en) 1997-02-12 2004-08-11 Eugene Y. Chan Methods for analyzimg polymers
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20030027203A1 (en) 1997-03-24 2003-02-06 Fields Robert E. Biomolecular processor
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
CA2287570C (en) 1997-05-02 2008-10-28 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
CA2286601A1 (en) 1997-05-16 1998-11-26 Alberta Research Council Microfluidic system and methods of use
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US20040241759A1 (en) 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
EP2258846A3 (en) 1997-07-07 2012-03-21 Medical Research Council A method for increasing the concentration of a nucleic acid molecule
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
CA2300940A1 (en) 1997-08-15 1999-02-25 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
WO1999014368A2 (en) 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
WO1999018438A1 (en) 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
AU737174B2 (en) 1997-10-10 2001-08-09 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
IL135593A (en) 1997-10-14 2004-06-01 Luminex Corp Precisely fluorescently painted polymeric microspheres and methods for making and using them
JP2001521169A (ja) 1997-10-24 2001-11-06 ノースイースタン・ユニバーシティ 広範囲の採集と分析とで高スループットの調整分離のためのマルチチャンネルの微小規模システム
EP1036332B1 (en) 1997-12-04 2005-07-13 Amersham Biosciences UK Limited Multiple assay method
WO1999033963A1 (en) 1997-12-31 1999-07-08 Chiron Corporation Metastatic cancer regulated gene
WO1999042597A1 (en) 1998-02-19 1999-08-26 President And Fellows Of Harvard College Monovalent, multivalent, and multimeric mhc binding domain fusion proteins and conjugates, and uses therefor
AU3196099A (en) 1998-03-27 1999-10-18 President And Fellows Of Harvard College Systematic identification of essential genes by (in vitro) transposon mutagenesis
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
WO1999052708A1 (en) 1998-04-13 1999-10-21 Luminex Corporation Liquid labeling with fluorescent microparticles
US6048736A (en) 1998-04-29 2000-04-11 Kosak; Kenneth M. Cyclodextrin polymers for carrying and releasing drugs
US5997636A (en) 1998-05-01 1999-12-07 Instrumentation Technology Associates, Inc. Method and apparatus for growing crystals
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
EP2360271A1 (en) 1998-06-24 2011-08-24 Illumina, Inc. Decoding of array sensors with microspheres
EP1102864B1 (en) 1998-08-07 2006-05-24 Cellay LLC Gel microdrops in genetic analysis
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CN1326549A (zh) 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
US6489096B1 (en) 1998-10-15 2002-12-03 Princeton University Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays
SE9803614L (sv) 1998-10-19 2000-04-20 Muhammed Mamoun Förfarande och anordning för framställning av nanopartiklar
WO2000026412A1 (en) 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6635419B1 (en) 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
CA2362263A1 (en) * 1999-02-19 2000-08-24 Bioserv Ag Biodegradable composite material for the production of microcapsules
US6613513B1 (en) 1999-02-23 2003-09-02 Caliper Technologies Corp. Sequencing by incorporation
US7615373B2 (en) 1999-02-25 2009-11-10 Virginia Commonwealth University Intellectual Property Foundation Electroprocessed collagen and tissue engineering
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
DK2264189T3 (en) 1999-04-20 2014-12-08 Illumina Inc Detection of nucleic acid reactions of bead - arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6291243B1 (en) 1999-04-28 2001-09-18 The Board Of Trustees Of The Leland Stanford Jr. University P element derived vector and methods for its use
WO2000068671A2 (en) 1999-05-12 2000-11-16 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6846622B1 (en) 1999-05-26 2005-01-25 Oregon Health & Science University Tagged epitope protein transposable element
US20030124509A1 (en) 1999-06-03 2003-07-03 Kenis Paul J.A. Laminar flow patterning and articles made thereby
US7098032B2 (en) 2001-01-02 2006-08-29 Mirus Bio Corporation Compositions and methods for drug delivery using pH sensitive molecules
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis DETECTION OF NUCLEIC ACIDS
EP1248853A2 (en) 1999-08-20 2002-10-16 Luminex Corporation Liquid array technology
JP2003508763A (ja) 1999-08-27 2003-03-04 マトリックス テクノロジーズ コーポレイション 固体支持体上にリガンドを固定化する方法及び装置並びにその使用方法
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
MXPA02003815A (es) 1999-10-13 2002-09-30 Signature Bioscience Inc Sistema y metodo para detectar e identificar eventos moleculares en una muestra de prueba.
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU1100201A (en) 1999-10-28 2001-05-08 Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
HK1052203A1 (zh) 1999-11-08 2003-09-05 荣研化学株式会社 检测突变和/或多态性的方法
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20010051348A1 (en) 2000-01-28 2001-12-13 Lee Chee Wee Novel ligands and methods for preparing same
US6720157B2 (en) 2000-02-23 2004-04-13 Zyomyx, Inc. Chips having elevated sample surfaces
WO2001063270A1 (en) 2000-02-23 2001-08-30 Caliper Technologies, Inc. Multi-reservoir pressure control system
IL134830A0 (en) 2000-03-01 2001-05-20 Chay 13 Medical Res Group N V Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them
EP1267912A2 (en) 2000-03-14 2003-01-02 Burkhard Göke Effects of glucagon-like peptide-1 (7-36) on antro-pyloro-duodenal motility
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
JP4927287B2 (ja) 2000-03-31 2012-05-09 マイクロニックス、インコーポレーテッド タンパク質の結晶化のマイクロ流動体装置
WO2001077683A1 (en) 2000-04-06 2001-10-18 Caliper Technologies Corp. Methods and devices for achieving long incubation times in high-throughput systems
DK2278029T3 (en) 2000-04-10 2016-10-03 Taxon Biosciences Inc Methods of study and genetic analysis of populations
US6481453B1 (en) 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US6800298B1 (en) 2000-05-11 2004-10-05 Clemson University Biological lubricant composition and method of applying lubricant composition
US20060008799A1 (en) 2000-05-22 2006-01-12 Hong Cai Rapid haplotyping by single molecule detection
US20020003001A1 (en) 2000-05-24 2002-01-10 Weigl Bernhard H. Surface tension valves for microfluidic applications
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US6632606B1 (en) 2000-06-12 2003-10-14 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
DE60117556T2 (de) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
EP1309404A2 (en) 2000-08-07 2003-05-14 Nanostream, Inc. Fluidic mixer in microfluidic system
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
EP1317569B1 (en) 2000-09-14 2009-11-18 Caliper Life Sciences, Inc. Microfluidic devices and methods for performing temperature mediated reactions
EP1334347A1 (en) 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods
WO2002029106A2 (en) 2000-10-03 2002-04-11 California Institute Of Technology Microfluidic devices and methods of use
EP1364052A2 (en) 2000-10-10 2003-11-26 Diversa Corporation High throughput or capillary-based screening for a bioactivity or biomolecule
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
JP2002155305A (ja) 2000-11-14 2002-05-31 Akira Kawasaki 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子
US6492154B2 (en) 2001-01-31 2002-12-10 Applera Corporation Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof
CA2332186A1 (en) 2001-02-08 2002-08-08 Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada Replicative in vivo gene targeting
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
EP1368309B2 (en) * 2001-02-22 2014-10-01 Anika Therapeutics Inc. Thiol-modified hyaluronan
DE60211857T2 (de) 2001-02-23 2006-12-21 Japan Science And Technology Agency, Kawaguchi Verfahren zur herstellung einer emulsion und vorrichtung dafür
US20150329617A1 (en) 2001-03-14 2015-11-19 Dynal Biotech Asa Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20060040286A1 (en) 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
EP1377821A2 (en) 2001-04-03 2004-01-07 Micronics, Inc. Pneumatic valve interface for use in microfluidic structures
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US20030027221A1 (en) * 2001-04-06 2003-02-06 Scott Melissa E. High-throughput screening assays by encapsulation
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
JP4194934B2 (ja) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド カプセル化細胞によるタンパク質の分泌法
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US6524259B2 (en) * 2001-06-08 2003-02-25 Cervilenz, Inc. Devices and methods for cervix measurement
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US7682353B2 (en) 2001-06-29 2010-03-23 Coloplast A/S Catheter device
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7077152B2 (en) 2001-07-07 2006-07-18 Nanostream, Inc. Microfluidic metering systems and methods
CA2454573A1 (en) 2001-07-20 2003-01-30 California Institute Of Technology Protein and nucleic acid expression systems
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
WO2003044221A1 (en) 2001-10-19 2003-05-30 West Virginia University Research Corporation Microfluidic system for proteome analysis
US20030149307A1 (en) 2001-10-24 2003-08-07 Baxter International Inc. Process for the preparation of polyethylene glycol bis amine
JP2005514224A (ja) 2001-10-26 2005-05-19 アクララ バイオサイエンシーズ， インコーポレイテッド ミクロ流体基材の射出成形ミクロ複製のためのシステムおよび方法
EP1448789A4 (en) 2001-10-30 2007-01-03 Nanomics Biosystems Pty Ltd DEVICE AND METHOD FOR THE TARGETED SYNTHESIS OF CHEMICAL LIBRARIES
US7262056B2 (en) 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
ATE458832T1 (de) 2001-11-28 2010-03-15 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
WO2003057010A2 (en) 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Droplet-based microfluidic oligonucleotide synthesis engine
WO2003062462A2 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
KR100459870B1 (ko) 2002-02-22 2004-12-04 한국과학기술원 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
DE60321325D1 (de) 2002-03-20 2008-07-10 Innovativebio Biz Kowloon Mikrokapseln mit kontrollierter durchlässigkeit die ein nukleinsäureamplifizierungsreaktionsgemisch enthalten und deren benutzung als reaktionsgefäss für parallele reaktionen
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
EP2278337B1 (en) 2002-05-09 2019-06-26 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US7927791B2 (en) * 2002-07-24 2011-04-19 Ptc Therapeutics, Inc. Methods for identifying small molecules that modulate premature translation termination and nonsense mediated mRNA decay
IL151660A0 (en) 2002-09-09 2003-04-10 Univ Ben Gurion Method for isolating and culturing unculturable microorganisms
CA2500129C (en) 2002-09-30 2011-03-29 F. Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US20040081962A1 (en) 2002-10-23 2004-04-29 Caifu Chen Methods for synthesizing complementary DNA
US6979713B2 (en) 2002-11-25 2005-12-27 3M Innovative Properties Company Curable compositions and abrasive articles therefrom
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20040248299A1 (en) 2002-12-27 2004-12-09 Sumedha Jayasena RNA interference
WO2004065617A2 (en) 2003-01-17 2004-08-05 The Trustees Of Boston University Haplotype analysis
US7244567B2 (en) 2003-01-29 2007-07-17 454 Life Sciences Corporation Double ended sequencing
DK1594973T3 (da) 2003-02-10 2012-02-13 Max Delbrueck Centrum Transposonbaseret målretningssystem
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7316903B2 (en) 2003-03-28 2008-01-08 United States Of America As Represented By The Department Of Health And Human Services Detection of nucleic acid sequence variations using phase Mu transposase
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2004087204A2 (en) 2003-04-04 2004-10-14 Pfizer Products Inc. Microfluidized oil-in-water emulsions and vaccine compositions
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
AU2004229440B2 (en) 2003-04-10 2010-08-12 President And Fellows Of Harvard College Formation and control of fluidic species
EP1629286A1 (en) 2003-05-16 2006-03-01 Global Technologies (NZ) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
DE112004001376D2 (de) 2003-05-19 2006-04-13 Knoell Hans Forschung Ev Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten
WO2004105734A1 (en) 2003-05-28 2004-12-09 Valorisation Recherche, Societe En Commandite Method of preparing microcapsules
GB0313170D0 (en) 2003-06-09 2003-07-16 Qinetiq Ltd Method and apparatus for spore disruption and/or detection
WO2004113877A1 (en) 2003-06-13 2004-12-29 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
CN101001960A (zh) * 2003-06-27 2007-07-18 西北大学 基于生物条形码检测靶分析物
GB2403475B (en) 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
EP2662136A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
EP1660153A2 (en) 2003-09-04 2006-05-31 The United States of America as represented by the Department of Veterans Affairs Hydrogel nanocompsites for ophthalmic applications
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005028646A1 (ja) 2003-09-22 2005-03-31 Riken 効率的なdna逆位反復構造の調製方法
KR101111231B1 (ko) 2003-09-25 2012-02-17 비바리스 마이크로 웰 어레이 칩 및 그 제조방법
WO2005047521A2 (en) 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
US20050136417A1 (en) 2003-12-19 2005-06-23 Affymetrix, Inc. Amplification of nucleic acids
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
ES2432040T3 (es) 2004-01-28 2013-11-29 454 Life Sciences Corporation Amplificación de ácido nucleico con emulsión de flujo continuo
US20050181379A1 (en) 2004-02-18 2005-08-18 Intel Corporation Method and device for isolating and positioning single nucleic acid molecules
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
CN100431679C (zh) 2004-03-23 2008-11-12 独立行政法人科学技术振兴机构 微小液滴的生成方法及装置
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20060020371A1 (en) 2004-04-13 2006-01-26 President And Fellows Of Harvard College Methods and apparatus for manipulation and/or detection of biological samples and other objects
US20050250147A1 (en) 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
US20080268507A1 (en) 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US7968085B2 (en) 2004-07-05 2011-06-28 Ascendis Pharma A/S Hydrogel formulations
CN1648671B (zh) 2005-02-06 2012-09-26 成都夸常医学工业有限公司 多反应器分析芯片检测方法和分析芯片及检测装置
JP4189760B2 (ja) * 2004-07-28 2008-12-03 アイシン精機株式会社 リクライニング装置
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
US20080268431A1 (en) 2004-09-14 2008-10-30 Jin-Ho Choy Information Code System Using Dna Sequences
US7892731B2 (en) 2004-10-01 2011-02-22 Radix Biosolutions, Ltd. System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007001448A2 (en) 2004-11-04 2007-01-04 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
US7329493B2 (en) 2004-12-22 2008-02-12 Asiagen Corporation One-tube nested PCR for detecting Mycobacterium tuberculosis
CA2592204C (en) 2004-12-23 2013-03-12 I-Stat Corporation Nucleic acid diagnostics system and methods
US20080213593A1 (en) 2005-01-21 2008-09-04 President And Fellows Of Harvard College Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006089192A2 (en) 2005-02-18 2006-08-24 Canon U.S. Life Sciences, Inc. Devices and methods for identifying genomic dna of organisms
WO2006088123A1 (ja) 2005-02-21 2006-08-24 Kagoshima University バイオディーゼル燃料の精製方法
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP2006289250A (ja) 2005-04-08 2006-10-26 Kao Corp マイクロミキサー及びそれを用いた流体混合方法
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
DK2620510T4 (da) 2005-06-15 2020-03-30 Complete Genomics Inc Enkeltmolekyle-arrays til genetisk og kemisk analyse
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
WO2007002490A2 (en) 2005-06-22 2007-01-04 The Research Foundation Of State University Of New York Massively parallel 2-dimensional capillary electrophoresis
US20070154903A1 (en) 2005-06-23 2007-07-05 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
EP1921140B1 (en) 2005-07-05 2011-12-14 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Mutant transposon vector and use thereof
JP5051490B2 (ja) * 2005-07-08 2012-10-17 独立行政法人産業技術総合研究所 マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
FR2888912B1 (fr) 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
WO2007024840A2 (en) 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
JP2007074967A (ja) 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
CN101523156A (zh) * 2005-09-16 2009-09-02 加利福尼亚大学董事会 用于分析物检测的比色生物条形码扩增测定
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070111241A1 (en) 2005-10-14 2007-05-17 Nezih Cereb System and method for accessing, tracking, and editing sequence analysis and software to accomplish the same
US7709544B2 (en) 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
EP1954838B1 (en) 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
US20070134277A1 (en) 2005-12-09 2007-06-14 Children's Medical Center Corporation Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms
US20070141584A1 (en) 2005-12-20 2007-06-21 Roberts Douglas N Methods for assessment of native chromatin on microarrays
US7932037B2 (en) 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
EP2364774A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US7544473B2 (en) 2006-01-23 2009-06-09 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070195127A1 (en) 2006-01-27 2007-08-23 President And Fellows Of Harvard College Fluidic droplet coalescence
EP2423334A3 (en) 2006-02-02 2012-04-18 The Board of Trustees of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603251D0 (en) 2006-02-17 2006-03-29 Isis Innovation DNA conformation
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
JP4921829B2 (ja) 2006-03-30 2012-04-25 株式会社東芝 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法
US20090181864A1 (en) 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
US20090062129A1 (en) 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7811603B2 (en) 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
EP2030011B1 (en) 2006-05-22 2017-02-01 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
RU2321638C2 (ru) 2006-05-23 2008-04-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Способ изготовления многофункционального мультичипа, мультичип для последовательного или параллельного скрининга биополимеров, способ анализа биополимеров и набор для осуществления способа
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
CN108048549B (zh) 2006-06-14 2021-10-26 维里纳塔健康公司 使用样品拆分和dna标签进行稀有细胞分析
CA2656022C (en) 2006-06-19 2017-10-17 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US20080076909A1 (en) 2006-06-30 2008-03-27 Applera Corporation Emulsion pcr and amplicon capture
EP1878501A1 (en) 2006-07-14 2008-01-16 Roche Diagnostics GmbH Instrument for heating and cooling
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
JP5553602B2 (ja) 2006-09-06 2014-07-16 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド マイクロ流体アッセイを実施するためのチップ及びカートリッジ設計構成
AU2007302586B2 (en) 2006-09-25 2013-06-27 Archer-Daniels-Midland Company Superabsorbent surface-treated carboxyalkylated polysaccharides and process for producing same
US7935518B2 (en) 2006-09-27 2011-05-03 Alessandra Luchini Smart hydrogel particles for biomarker harvesting
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
EP2518162B1 (en) 2006-11-15 2018-03-07 Biospherex LLC Multitag sequencing and ecogenomics analysis
JP2010509995A (ja) 2006-11-20 2010-04-02 ネイティヴィス、インコーポレイテッド 低周波信号によってインビトロ・システム又は哺乳類システムをトランスデュースするための装置および方法
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
JP5320546B2 (ja) 2006-12-13 2013-10-23 国立大学法人名古屋大学 Ｔｏｌ１因子のトランスポザーゼ及びそれを用いたＤＮＡ導入システム
JP5622392B2 (ja) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション 大規模ｆｅｔアレイを用いた分析物測定のための方法および装置
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
JP2008167722A (ja) 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
US7844658B2 (en) 2007-01-22 2010-11-30 Comcast Cable Holdings, Llc System and method for providing an application to a device
US20080176768A1 (en) 2007-01-23 2008-07-24 Honeywell Honeywell International Hydrogel microarray with embedded metal nanoparticles
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20080228268A1 (en) 2007-03-15 2008-09-18 Uluru, Inc. Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis
US7943330B2 (en) 2007-03-23 2011-05-17 Academia Sinica Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins
CN102014871A (zh) 2007-03-28 2011-04-13 哈佛大学 乳液及其形成技术
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
CN101293191B (zh) 2007-04-25 2011-11-09 中国科学院过程工程研究所 一种琼脂糖凝胶微球的制备方法
WO2008135512A2 (en) 2007-05-02 2008-11-13 Jerzy Paszkowski Dna amplification method
US20080295909A1 (en) 2007-05-24 2008-12-04 Locascio Laurie E Microfluidic Device for Passive Sorting and Storage of Liquid Plugs Using Capillary Force
CA2689356A1 (en) 2007-06-01 2008-12-11 454 Life Sciences Corporation System and meth0d for identification of individual samples from a multiplex mixture
CA2689427C (en) 2007-06-05 2015-12-29 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
US20100255556A1 (en) 2007-06-29 2010-10-07 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US20130084243A1 (en) 2010-01-27 2013-04-04 Liliane Goetsch Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders
AU2008286737A1 (en) 2007-08-15 2009-02-19 New York University Method, system and software arrangement for comparative analysis and phylogeny with whole-genome optical maps
US8563527B2 (en) * 2007-08-20 2013-10-22 Pharmain Corporation Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2009048532A2 (en) 2007-10-05 2009-04-16 President And Fellows Of Harvard College Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
US20090099040A1 (en) 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US20100086914A1 (en) 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
JP2011500092A (ja) 2007-10-26 2011-01-06 ロゼッタ、インファーマティクス、リミテッド、ライアビリティ、カンパニー 非ランダムプライマーを用いたｃＤＮＡ合成の方法
WO2009061372A1 (en) 2007-11-02 2009-05-14 President And Fellows Of Harvard College Systems and methods for creating multi-phase entities, including particles and/or fluids
US8334013B2 (en) 2007-11-02 2012-12-18 Stc.Unm Mesoporous metal oxide microspheres and method for forming same
US8592150B2 (en) * 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20110008775A1 (en) * 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
US7771944B2 (en) 2007-12-14 2010-08-10 The Board Of Trustees Of The University Of Illinois Methods for determining genetic haplotypes and DNA mapping
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
EP3360972B1 (en) 2008-01-17 2019-12-11 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes
KR20090081260A (ko) 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
EP2252625B1 (en) 2008-02-07 2017-12-20 Pacific Biosciences of California, Inc. Cis reactive oxygen quenchers integrated into linkers
JP5468271B2 (ja) 2008-02-08 2014-04-09 花王株式会社 微粒子分散液の製造方法
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
KR101030476B1 (ko) 2008-03-11 2011-04-25 국립암센터 Ｓｎｐ 어레이를 이용한 염색체, 유전자, 또는 특정 뉴클레오티드 서열의 카피 수 측정방법
CN101241126A (zh) 2008-03-14 2008-08-13 东华大学 一种生物条形码探针的制备方法
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
GB0810051D0 (en) 2008-06-02 2008-07-09 Oxford Biodynamics Ltd Method of diagnosis
US20110305761A1 (en) 2008-06-05 2011-12-15 President And Fellows Of Harvard College Polymersomes, colloidosomes, liposomes, and other species associated with fluidic droplets
EP2291533B2 (en) 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
EP2303245B1 (en) * 2008-07-11 2016-12-28 ETH Zurich Degradable microcapsules
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010009735A2 (en) 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US20100062494A1 (en) 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US8709762B2 (en) * 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9822393B2 (en) 2013-03-08 2017-11-21 Bio-Rad Laboratories, Inc. Compositions, methods and systems for polymerase chain reaction assays
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
MX2011002936A (es) 2008-09-25 2011-04-11 Cephalon Inc Formulaciones liquidas de bendamustina.
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
HUE027961T2 (en) 2008-10-24 2016-11-28 Epicentre Tech Corp Transposon final preparations and methods for modifying nucleic acids
US20100113296A1 (en) 2008-11-05 2010-05-06 Joel Myerson Methods And Kits For Nucleic Acid Analysis
EP3699296A1 (en) 2008-11-07 2020-08-26 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US20100203647A1 (en) 2008-11-21 2010-08-12 The Rockefeller University Chemical Reporters of Protein Acylation
CA2749570A1 (en) 2008-12-02 2010-06-10 Bio-Rad Laboratories, Inc. Chromatin structure detection
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
WO2010075570A2 (en) 2008-12-24 2010-07-01 New York University Methods, computer-accessible medium, and systems for score-driven whole-genome shotgun sequence assemble
KR101065807B1 (ko) 2009-01-23 2011-09-19 충남대학교산학협력단 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
BRPI1008965B1 (pt) 2009-03-13 2018-12-18 Harvard College método para aumento de escala de dispositivos microfluídicos e sistema para a formação de gotículas em canais microfluídicos em paralelo
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP3495498B1 (en) 2009-03-30 2021-10-27 Illumina, Inc. Gene expression analysis in single cells
DK2414547T3 (da) 2009-04-02 2014-06-16 Fluidigm Corp Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20100279882A1 (en) 2009-05-01 2010-11-04 Mostafa Ronaghi Sequencing methods
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
FR2945545B1 (fr) 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
FR2945819B1 (fr) 2009-05-19 2011-06-17 Commissariat Energie Atomique Dispositif et procede d'isolement de cibles biologiques ou chimiques
US8574835B2 (en) * 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US9757698B2 (en) 2009-06-26 2017-09-12 President And Fellows Of Harvard College Fluid injection
US20110028412A1 (en) 2009-08-03 2011-02-03 Cappellos, Inc. Herbal enhanced analgesic formulations
US20110033548A1 (en) * 2009-08-05 2011-02-10 E.I. Du Pont De Nemours And Company Degradable crosslinked aminated dextran microspheres and methods of use
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
CN102482716A (zh) 2009-09-01 2012-05-30 皇家飞利浦电子股份有限公司 用于微阵列选择的设备和方法
CA3106547C (en) * 2009-09-02 2022-07-05 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
WO2011028764A2 (en) 2009-09-02 2011-03-10 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
GB0918564D0 (en) * 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
EP3461558B1 (en) 2009-10-27 2021-03-17 President and Fellows of Harvard College Droplet creation techniques
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
CN107028886A (zh) 2009-11-04 2017-08-11 不列颠哥伦比亚大学 含有核酸的脂质粒子及相关的方法
WO2011066476A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
CN102985552B (zh) 2009-11-25 2016-02-17 伯乐生命医学产品有限公司 用于检测遗传物质的方法和组合物
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP2013514079A (ja) 2009-12-17 2013-04-25 キージーン・エン・フェー 制限酵素に基づく全ゲノムシーケンシング
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP3392349A1 (en) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (ja) 2010-02-19 2016-04-06 国立大学法人 千葉大学 ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント
JP6137834B2 (ja) 2010-02-24 2017-05-31 アローヘッド ファーマシューティカルズ インコーポレイテッド ｓｉＲＮＡの標的化送達のための組成物
US20110257889A1 (en) 2010-02-24 2011-10-20 Pacific Biosciences Of California, Inc. Sequence assembly and consensus sequence determination
EP3072968A1 (en) 2010-02-25 2016-09-28 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US10500156B2 (en) 2010-03-24 2019-12-10 Northeastern University Multi-compartmental macrophage delivery
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
FR2958186A1 (fr) 2010-03-30 2011-10-07 Ecole Polytech Dispositif de formation de gouttes dans un circuit microfluide.
EP2556171B1 (en) 2010-04-05 2015-09-02 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9255291B2 (en) 2010-05-06 2016-02-09 Bioo Scientific Corporation Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
US20110287947A1 (en) 2010-05-18 2011-11-24 University Of Southern California Tethered Conformation Capture
CN107029223A (zh) 2010-05-26 2017-08-11 西莱克塔生物科技公司 合成纳米载体联合疫苗
US20120000777A1 (en) 2010-06-04 2012-01-05 The Regents Of The University Of California Devices and methods for forming double emulsion droplet compositions and polymer particles
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
US8703493B2 (en) 2010-06-15 2014-04-22 Src, Inc. Location analysis using fire retardant-protected nucleic acid-labeled tags
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
CN103202812B (zh) 2010-08-09 2015-10-28 南京大学 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法
WO2012019765A1 (en) 2010-08-10 2012-02-16 European Molecular Biology Laboratory (Embl) Methods and systems for tracking samples and sample combinations
CN103328007B (zh) 2010-09-16 2016-09-21 北卡罗来纳州大学查珀尔希尔分校 作为药物试剂、化学试剂和生物试剂的递送载剂和前药的不对称双官能甲硅烷基单体及其颗粒
CN102409048B (zh) 2010-09-21 2013-10-23 深圳华大基因科技服务有限公司 一种基于高通量测序的dna标签文库构建方法
DK2623613T3 (en) 2010-09-21 2016-10-03 Population Genetics Tech Ltd Increasing the reliability of the allele-indications by molecular counting
CN103328981B (zh) 2010-10-04 2017-04-12 吉纳普赛斯股份有限公司 用于自动化可重复使用的平行生物反应的系统和方法
US9999886B2 (en) 2010-10-07 2018-06-19 The Regents Of The University Of California Methods and systems for on demand droplet generation and impedance based detection
DK2625320T3 (da) 2010-10-08 2019-07-01 Harvard College High-throughput enkeltcellestregkodning
EP2625295B1 (en) 2010-10-08 2019-03-13 President and Fellows of Harvard College High-throughput immune sequencing
CN102050953B (zh) 2010-10-18 2012-11-07 武汉理工大学 一种可还原降解超分子水凝胶的制备方法
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
CA3024250C (en) 2010-11-01 2022-01-04 Bio-Rad Laboratories, Inc. System for forming emulsions
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
US20140057799A1 (en) 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US20120184449A1 (en) 2010-12-23 2012-07-19 Sequenom, Inc. Fetal genetic variation detection
WO2012100216A2 (en) 2011-01-20 2012-07-26 Knome, Inc. Methods and apparatus for assigning a meaningful numeric value to genomic variants, and searching and assessing same
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
GB201101429D0 (en) 2011-01-27 2011-03-16 Biocompatibles Uk Ltd Drug delivery system
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ， インコーポレイテッド 細胞において複数のエピトープを同定する方法
AU2012212148B8 (en) * 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
EP3483285B1 (en) 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP2678326B1 (en) 2011-02-25 2017-08-02 University of Massachusetts Medical School Monomers and polymers for functional polycarbonates and poly (ester-carbonates) and peg-co-polycarbonate hydrogels
US20140045706A1 (en) 2011-02-25 2014-02-13 Illumina, Inc. Methods and systems for haplotype determination
EP2966061B1 (en) 2011-03-04 2017-05-03 Life Technologies Corporation Compounds and methods for conjugation of biomolecules
US20120233201A1 (en) 2011-03-09 2012-09-13 Annai Systems, Inc. Biological data networks and methods therefor
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB2489714B (en) * 2011-04-05 2013-11-06 Tracesa Ltd Fluid Identification Method
WO2012150317A1 (en) 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CA2833165A1 (en) 2011-04-14 2012-10-18 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
EP3789498B1 (en) * 2011-04-25 2025-08-13 Bio-Rad Laboratories, Inc. Methods for nucleic acid analysis
EP3495497B1 (en) 2011-04-28 2021-03-24 Life Technologies Corporation Methods and compositions for multiplex pcr
CA3078215A1 (en) 2011-04-28 2012-11-01 Department Of Veterans Affairs Identification of polynucleotides associated with a sample
JP6100685B2 (ja) 2011-05-16 2017-03-22 地方独立行政法人 大阪府立病院機構 血中ｄｎａの定量的検出による悪性新生物の病勢の進行を評価する方法
CA2836361C (en) 2011-05-16 2020-11-10 Koninklijke Philips N.V. Bio-orthogonal drug activation
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9150916B2 (en) 2011-06-24 2015-10-06 Beat Christen Compositions and methods for identifying the essential genome of an organism
US8927218B2 (en) 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
WO2013006824A2 (en) 2011-07-07 2013-01-10 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9605304B2 (en) 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
JP6158808B2 (ja) 2011-08-04 2017-07-05 セイジ サイエンス，インコーポレイティド 流体を処理するためのシステム及び方法
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
US20120048340A1 (en) * 2011-08-09 2012-03-01 General Electric Company Solar panel tracking system and associated tracking sensor
US8846883B2 (en) 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
WO2013035114A1 (en) 2011-09-08 2013-03-14 Decode Genetics Ehf Tp53 genetic variants predictive of cancer
HK1199745A1 (en) 2011-09-09 2015-07-17 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
LT2768607T (lt) 2011-09-26 2021-12-27 Thermo Fisher Scientific Geneart Gmbh Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti
CN103958050B (zh) 2011-09-28 2016-09-14 哈佛学院院长等 用于液滴产生和/或流体操纵的系统和方法
US9514272B2 (en) 2011-10-12 2016-12-06 Complete Genomics, Inc. Identification of DNA fragments and structural variations
US9469874B2 (en) 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US20130109576A1 (en) 2011-10-28 2013-05-02 Anthony P. Shuber Methods for detecting mutations
WO2013066416A1 (en) 2011-11-04 2013-05-10 Intel Corporation Signaling for configuration of downlink coordinated multipoint communications
JP6083713B2 (ja) 2011-11-16 2017-02-22 インターナショナル・ビジネス・マシーンズ・コーポレーションＩｎｔｅｒｎａｔｉｏｎａｌ Ｂｕｓｉｎｅｓｓ Ｍａｃｈｉｎｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 変形可能バルブを備えたマイクロ流体デバイス
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
SG10201609393QA (en) 2011-12-03 2017-01-27 Emd Millipore Corp Micro-incubation systems for microfluidic cell culture and methods
ES2952728T3 (es) 2011-12-22 2023-11-03 Harvard College Métodos para la detección de analitos
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
EP2812360B1 (en) 2012-02-09 2022-08-31 Life Technologies Corporation Hydrophilic polymeric particles and methods for making same
CN104271768A (zh) 2012-02-14 2015-01-07 约翰霍普金斯大学 miRNA分析方法
US9090662B2 (en) 2012-02-15 2015-07-28 Wisconsin Alumni Research Foundation Dithioamine reducing agents
DK2814959T3 (en) 2012-02-17 2018-04-23 Hutchinson Fred Cancer Res COMPOSITIONS AND PROCEDURES FOR EXACTLY IDENTIFYING MUTATIONS
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP3309262B1 (en) 2012-02-24 2019-09-25 Bio-Rad Laboratories, Inc. Labeling and sample preparation for sequencing
EP3305918B1 (en) 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
NO2694769T3 (enExample) 2012-03-06 2018-03-03
US9552458B2 (en) 2012-03-16 2017-01-24 The Research Institute At Nationwide Children's Hospital Comprehensive analysis pipeline for discovery of human genetic variation
WO2013148189A1 (en) 2012-03-30 2013-10-03 Massachusetts Institute Of Technology Probe incorporation mediated by enzymes
CN102622634B (zh) * 2012-03-31 2014-04-23 中国农业科学院果树研究所 一种苹果种质资源条形码标识的制备方法
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
MX356508B (es) 2012-04-16 2018-05-30 Biological dynamics inc Preparacion de muestras de acido nucleico.
US20130296173A1 (en) 2012-04-23 2013-11-07 Complete Genomics, Inc. Pre-anchor wash
CA2874413A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
WO2013188831A1 (en) 2012-06-15 2013-12-19 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
EP2861760B1 (en) 2012-06-15 2020-01-01 The Board of Regents of The University of Texas System High throughput sequencing of multiple transcripts of a single cell
SG11201500313YA (en) 2012-07-24 2015-02-27 Sequenta Inc Single cell analysis using sequence tags
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
EP4001426A1 (en) 2012-08-13 2022-05-25 The Regents of The University of California Methods and systems for detecting biological components
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2885418A4 (en) 2012-08-14 2016-03-02 10X Genomics Inc MICROCAPSE COMPOSITIONS AND METHOD THEREFOR
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20220098659A1 (en) 2012-08-14 2022-03-31 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
WO2014047561A1 (en) 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
FR2996544B1 (fr) 2012-10-08 2015-03-13 Ecole Polytech Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant.
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
CN107541546B (zh) 2012-10-15 2021-06-15 生命技术公司 用于标靶核酸富集的组合物、方法、系统和试剂盒
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN107090491A (zh) 2012-11-05 2017-08-25 鲁比康基因组学公司 条形编码核酸
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
EP2917368A1 (en) 2012-11-07 2015-09-16 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
US20150299772A1 (en) 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
IL307735A (en) 2012-12-12 2023-12-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2749653A1 (en) 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Molecular coding for analysis of composition of macromolecules and molecular complexes
EP2752664A1 (en) 2013-01-07 2014-07-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Label-free method for the detection of analytes
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014113736A1 (en) 2013-01-17 2014-07-24 Edico Genome Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
KR101984699B1 (ko) 2013-01-24 2019-05-31 삼성전자주식회사 핵산 분석용 미세 유체 시스템
CN105074303B (zh) 2013-01-25 2018-04-10 基纽拜奥股份有限公司 实施液滴膨胀的系统和方法
US10381106B2 (en) 2013-01-28 2019-08-13 Hasso-Plattner-Institut Fuer Softwaresystemtechnik Gmbh Efficient genomic read alignment in an in-memory database
US11110458B2 (en) 2013-02-01 2021-09-07 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
JP2016514047A (ja) 2013-03-06 2016-05-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 比較的単分散の液滴を形成するためのデバイスおよび方法
CA3094792A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014152155A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Massively multiplexed rna sequencing
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
WO2014145760A1 (en) 2013-03-15 2014-09-18 Bio-Rad Laboratories, Inc. Droplet generator with collection tube
WO2014145047A1 (en) 2013-03-15 2014-09-18 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
CA2906076A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
HK1218768A1 (zh) 2013-03-15 2017-03-10 Sygnis Biotech S.L.U. 使用热稳定的tthprimpol扩增和测序的方法
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
CA3156663A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014165559A2 (en) 2013-04-02 2014-10-09 Raindance Technologies, Inc. Systems and methods for handling microfluidic droplets
WO2014182835A1 (en) 2013-05-09 2014-11-13 Bio-Rad Laboratories, Inc. Magnetic immuno digital pcr assay
CN111394426B (zh) 2013-05-23 2024-05-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
ES2754177T3 (es) 2013-06-12 2020-04-16 Massachusetts Gen Hospital Métodos para la detección multiplexada de moléculas diana y sus usos
SG11201510284XA (en) 2013-06-17 2016-01-28 Broad Inst Inc Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
AU2014302277A1 (en) 2013-06-27 2015-12-24 10X Genomics, Inc. Compositions and methods for sample processing
WO2015006700A1 (en) 2013-07-12 2015-01-15 University Of South Alabama Minimal piggybac vectors for genome integration
CN103394410B (zh) 2013-07-25 2016-04-20 博奥生物集团有限公司 一种位置可调节的智能磁力架
GB2516684A (en) 2013-07-30 2015-02-04 Sphere Fluidics Ltd Microfluidic devices and systems
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9381217B2 (en) 2013-09-09 2016-07-05 Georgia Tech Research Corporation Microgels for encapsulation of cells and other biologic agents
WO2015048173A2 (en) 2013-09-24 2015-04-02 The Regents Of The University Of California Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
CA2926934A1 (en) 2013-10-09 2015-04-16 Stc.Unm Synthetic long read dna sequencing
WO2015065924A2 (en) 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US20140315755A1 (en) 2013-12-26 2014-10-23 Tao Chen Genome-wide Antisense Oligonucleotide and RNAi
CN106460033B (zh) 2013-12-30 2021-12-24 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
KR101464100B1 (ko) 2014-01-29 2014-11-21 성균관대학교산학협력단 생체 적용 가능한 리포솜-핵산형광 나노 융합체, 이의 응용, 및 이의 제조방법
US20170009288A1 (en) 2014-02-03 2017-01-12 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
CN105531408B (zh) 2014-02-13 2019-09-10 生物辐射实验室股份有限公司 染色体构象划分产物捕获
CA2940669C (en) 2014-02-27 2023-10-03 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
TWI682997B (zh) 2014-04-15 2020-01-21 美商伊路米納有限公司 用於改善插入序列傾向及增加ｄｎａ輸入耐受度之經修飾的轉位酶
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CA2946144C (en) 2014-04-21 2025-07-08 Harvard College SYSTEMS AND METHODS FOR MARKING NUCLEIC ACIDS WITH A BARCODE
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
EP3146046B1 (en) 2014-05-23 2020-03-11 Digenomix Corporation Haploidome determination by digitized transposons
DK3152232T3 (da) 2014-06-06 2020-02-24 Immudex Aps Bestemmelse af antigen-genkendelse ved stregkodning af MHC-multimerer
US10704080B2 (en) 2014-06-11 2020-07-07 Samplix Aps Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS)
US9534215B2 (en) 2014-06-11 2017-01-03 Life Technologies Corporation Systems and methods for substrate enrichment
WO2015188839A2 (en) 2014-06-13 2015-12-17 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US20150376605A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods and Compositions for Sample Analysis
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3161700B1 (en) 2014-06-26 2023-03-29 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
DK3656875T3 (da) 2014-07-18 2021-12-13 Illumina Inc Ikke-invasiv prænatal diagnostik
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
CN105335116B (zh) 2014-07-30 2018-11-09 联想(北京)有限公司 一种显示控制方法及电子设备
WO2016033251A2 (en) 2014-08-26 2016-03-03 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP3191605B1 (en) 2014-09-09 2022-07-27 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
EP3950944A1 (en) 2014-09-15 2022-02-09 AbVitro LLC High-throughput nucleotide library sequencing
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10783984B2 (en) 2014-12-18 2020-09-22 Pacific Biosciences Of California, Inc. De novo diploid genome assembly and haplotype sequence reconstruction
JP2017537645A (ja) 2014-12-19 2017-12-21 アプライズ バイオ， インコーポレイテッド 細胞の選択された部分集団中の複数エピトープを識別するための方法
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
EP4092681A1 (en) 2015-01-13 2022-11-23 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CN115011670A (zh) 2015-02-04 2022-09-06 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN107208156B (zh) 2015-02-09 2021-10-08 10X基因组学有限公司 用于使用变异识别数据来确定结构变异和定相的系统和方法
CA2975739C (en) 2015-02-10 2022-12-06 Illumina, Inc. Methods and compositions for analyzing cellular components
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
CN107614700A (zh) 2015-03-11 2018-01-19 布罗德研究所有限公司 基因型和表型偶联
WO2016149661A1 (en) 2015-03-18 2016-09-22 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
WO2016160908A1 (en) 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for combining single cell profiling with combinatorial nanoparticle conjugate library screening
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
JP2018511341A (ja) 2015-04-17 2018-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子配列決定および他の適用のためのバーコード化システムおよび方法
CN107250447B (zh) 2015-04-20 2020-05-05 深圳华大生命科学研究院 一种长片段dna文库构建方法
WO2016170126A1 (en) 2015-04-22 2016-10-27 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
US20160314242A1 (en) 2015-04-23 2016-10-27 10X Genomics, Inc. Sample indexing methods and compositions for sequencing applications
WO2016187256A2 (en) 2015-05-18 2016-11-24 10X Genomics, Inc. Mobile solid phase compositions for use in biochemical reactions and analyses
EP3298168A4 (en) 2015-05-18 2019-02-20 10X Genomics, Inc. STABILIZED REDUCING AGENTS AND METHOD FOR THEIR USE
WO2016187717A1 (en) 2015-05-26 2016-12-01 Exerkine Corporation Exosomes useful for genome editing
US20180087050A1 (en) 2015-05-27 2018-03-29 Jianbiao Zheng Methods of inserting molecular barcodes
MY194686A (en) 2015-06-24 2022-12-15 Oxford BioDynamics PLC Detection of chromosome interactions
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
SMT202000037T1 (it) 2015-08-12 2020-03-13 Cemm Forschungszentrum Fuer Molekulare Medizin Gmbh Metodi per studiare gli acidi nucleici
US11479805B2 (en) 2015-08-21 2022-10-25 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
BR112018005937A2 (pt) 2015-09-24 2019-05-21 Abvitro Llc conjugados de oligonucleotídeo de afinidade e usos destes
AU2016338907B2 (en) 2015-10-13 2022-07-07 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
US10900031B2 (en) 2015-10-19 2021-01-26 Zhejiang Annoroad Bio-Technology Co. Ltd. Method for constructing high-resolution single cell Hi-C library with a lot of information
CN108368522A (zh) 2015-10-20 2018-08-03 10X 基因组学有限公司 用于高通量单细胞遗传操作的方法和系统
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017079593A1 (en) 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
CN108350499B (zh) 2015-11-19 2022-05-13 10X基因组学有限公司 可转化标记组合物、方法及结合其的过程
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108779492A (zh) 2015-12-30 2018-11-09 生物辐射实验室股份有限公司 数字蛋白质定量
WO2017139690A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
CN109196115A (zh) 2016-03-01 2019-01-11 通用测序技术公司 跟踪核酸靶标来源以用于核酸测序的方法和试剂盒
EP3426774A4 (en) 2016-03-10 2019-08-14 The Board of Trustees of the Leland Stanford Junior University TRANSPOSASE-MEDIATED ILLUSTRATION OF ACCESSIBLE GENOMS
WO2017180420A1 (en) 2016-04-11 2017-10-19 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
US11925933B2 (en) 2016-04-15 2024-03-12 President And Fellows Of Harvard College Systems and methods for the collection of droplets and/or other entities
JP2019515674A (ja) 2016-04-19 2019-06-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
RU2019106038A (ru) 2016-08-10 2020-09-17 Президент Энд Фэллоуз Оф Харвард Коллидж Способы de novo сборки баркодированных фрагментов геномной днк
WO2018039338A1 (en) 2016-08-23 2018-03-01 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
US10858699B2 (en) 2016-08-30 2020-12-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
CN109923216B (zh) 2016-08-31 2024-08-02 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
RU2736351C2 (ru) 2016-08-31 2020-11-16 Президент Энд Фэллоуз Оф Харвард Коллидж Способы дискретной амплификации полного генома
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
BR112019005900A2 (pt) 2016-09-26 2019-06-11 Cellular Research, Inc. medição de expressão de proteínas usando reagentes com sequências de oligonucleotídeos com código de barras
EP4026905B1 (en) 2016-10-19 2024-04-17 10X Genomics, Inc. Methods for barcoding nucleic acid molecules from individual cells or cell populations
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US11434483B2 (en) 2016-12-07 2022-09-06 Mgi Tech Co., Ltd. Method for constructing single cell sequencing library and use thereof
CN118441026A (zh) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
EP3571308A4 (en) 2016-12-21 2020-08-19 The Regents of The University of California GENOMIC SEQUENCING OF SINGLE CELLS USING HYDROGEL-BASED DROPS
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20250005529A (ko) 2016-12-29 2025-01-09 일루미나, 인코포레이티드 세포 구획 내의 생체분자에 대한 직교 접근 및 그의 태그부착을 위한 분석 시스템
EP4095263A1 (en) 2017-01-06 2022-11-30 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US20210087610A9 (en) 2017-01-12 2021-03-25 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
EP4628582A2 (en) 2017-01-30 2025-10-08 10x Genomics, Inc. Methods and systems for droplet-based single cell barcoding
DK3583214T5 (da) 2017-02-02 2024-09-02 New York Genome Center Inc Fremgangsmåder og sammensætninger til identificering eller kvantificering af mål i en biologisk prøve
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10347365B2 (en) 2017-02-08 2019-07-09 10X Genomics, Inc. Systems and methods for visualizing a pattern in a dataset
GB201704402D0 (en) 2017-03-20 2017-05-03 Blacktrace Holdings Ltd Single cell DNA sequencing
KR20190133711A (ko) 2017-03-24 2019-12-03 싱가포르국립대학교 분자의 다중 검출 방법
EP3610034B1 (en) 2017-04-12 2022-06-08 Karius, Inc. Sample preparation methods, systems and compositions
US12054764B2 (en) 2017-04-14 2024-08-06 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4215616B1 (en) 2017-05-18 2024-09-04 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. DATA SET ANALYSIS SYSTEMS AND METHODS
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2018236615A1 (en) 2017-06-20 2018-12-27 10X Genomics, Inc. Methods and systems for improved droplet stabilization
WO2018237209A1 (en) 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
CN108336542B (zh) 2017-06-23 2020-02-21 番禺得意精密电子工业有限公司 电连接器
AU2018312560B2 (en) 2017-08-01 2022-03-10 Illumina, Inc. Hydrogel beads for nucleotide sequencing
US9946577B1 (en) 2017-08-14 2018-04-17 10X Genomics, Inc. Systems and methods for distributed resource management
US10821442B2 (en) 2017-08-22 2020-11-03 10X Genomics, Inc. Devices, systems, and kits for forming droplets
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019071039A1 (en) 2017-10-04 2019-04-11 10X Genomics, Inc. COMPOSITIONS, METHODS AND SYSTEMS FOR PEARL FORMATION USING ENHANCED POLYMERS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084328A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS FOR PREPARING NUCLEIC ACID MOLECULES
US20190127731A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods for preparing nucleic acid molecules
EP4241882B1 (en) 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
CN108173158B (zh) 2018-01-05 2023-09-08 浙江正泰电器股份有限公司 抽屉式断路器
WO2019148042A1 (en) 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
US12377396B2 (en) 2018-02-05 2025-08-05 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CN112074610A (zh) 2018-02-22 2020-12-11 10X基因组学有限公司 接合介导的核酸分析
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
WO2019191321A1 (en) 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190345636A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190352717A1 (en) 2018-05-18 2019-11-21 10X Genomics, Inc. Targeted non-invasive prenatal testing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US11574706B2 (en) 2018-06-28 2023-02-07 10X Genomics, Inc. Systems and methods for visualization of single-cell resolution characteristics
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20200033366A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US20200105373A1 (en) 2018-09-28 2020-04-02 10X Genomics, Inc. Systems and methods for cellular analysis using nucleic acid sequencing
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3906318A1 (en) 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3938537A1 (en) 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
EP4025709A1 (en) 2019-09-06 2022-07-13 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
CN115135984A (zh) 2019-12-23 2022-09-30 10X基因组学有限公司 可逆固定试剂及其使用方法
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021174051A1 (en) 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
CN115916972A (zh) 2020-04-16 2023-04-04 10X基因组学有限公司 用于被固定样品的组合物和方法
WO2021222302A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for increasing cell recovery efficiency
WO2021222301A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for analysis and identification of barcode multiplets
WO2022103712A1 (en) 2020-11-13 2022-05-19 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
EP4298239B1 (en) 2021-02-23 2025-11-05 10X Genomics, Inc. Drug screening methods
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4359755A1 (en) 2021-06-23 2024-05-01 10X Genomics, Inc. Chop-fix method and chopping device for preparing biological samples
EP4419911A4 (en) 2021-10-28 2025-03-05 10X Genomics, Inc. Methods for sample preparation
WO2024006392A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins

Also Published As

Publication number Publication date
WO2014028537A1 (en) 2014-02-20
AU2018203012A1 (en) 2018-05-17
CN111748607A (zh) 2020-10-09
AU2022200718B2 (en) 2024-03-14
AU2013302756A1 (en) 2015-02-26
CA3216609C (en) 2024-05-14
CN113528634A (zh) 2021-10-22
US20180258466A1 (en) 2018-09-13
US20170356027A1 (en) 2017-12-14
US20140155295A1 (en) 2014-06-05
CA2881685A1 (en) 2014-02-20
IN2015DN01126A (enExample) 2015-06-26
AU2013302756B2 (en) 2018-02-08
US11078522B2 (en) 2021-08-03
AU2020203602A1 (en) 2020-06-25
US20140287963A1 (en) 2014-09-25
AU2024203235A1 (en) 2024-06-06
MX2015001939A (es) 2015-09-23
AU2022200718A1 (en) 2022-02-24
US20200149090A1 (en) 2020-05-14
EP4397767A3 (en) 2024-07-31
AU2020203602B2 (en) 2022-01-27
US20150224466A1 (en) 2015-08-13
CA3216609A1 (en) 2014-02-20
AU2020203602C1 (en) 2022-05-05
KR20150048158A (ko) 2015-05-06
BR112015003354A2 (pt) 2017-08-08
EP2885418A4 (en) 2016-03-02
CN114891871A (zh) 2022-08-12
JP2015528283A (ja) 2015-09-28
BR112015003354A8 (pt) 2018-01-16
CN104769127A (zh) 2015-07-08
US20170321252A1 (en) 2017-11-09
US20180030512A1 (en) 2018-02-01
JP2018157832A (ja) 2018-10-11
US20220081704A1 (en) 2022-03-17
US20250129406A1 (en) 2025-04-24
US9695468B2 (en) 2017-07-04
CN111748607B (zh) 2024-04-30
KR102090851B1 (ko) 2020-03-19
CA2881685C (en) 2023-12-05
IL237156B (en) 2019-07-31
US9689024B2 (en) 2017-06-27
EP2885418A1 (en) 2015-06-24
US20180112253A1 (en) 2018-04-26
AU2018203012B2 (en) 2020-03-12
US12037634B2 (en) 2024-07-16
US10053723B2 (en) 2018-08-21
US20150225777A1 (en) 2015-08-13
IL237156A0 (en) 2015-04-30
US20150225778A1 (en) 2015-08-13
EP3901273A1 (en) 2021-10-27
MX364957B (es) 2019-05-15
EP4397767A2 (en) 2024-07-10
AU2013302756C1 (en) 2018-05-17

Similar Documents

Publication Publication Date Title
AU2022200718B2 (en) Microcapsule compositions and methods
CN113286893A (zh) 生成阵列的方法
CN115928221A (zh) 单细胞核酸序列分析
US20240271172A1 (en) Methods for processing nucleic acid samples

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160527

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170410

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170710

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170720

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20171204

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180226

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180604

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180704

R150 Certificate of patent or registration of utility model

Ref document number: 6367196

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250