FI117136B - Förfarande för framställning av terapeutiskt användbara analoger av erytropoietin - Google Patents
Förfarande för framställning av terapeutiskt användbara analoger av erytropoietin Download PDFInfo
- Publication number
- FI117136B FI117136B FI951792A FI951792A FI117136B FI 117136 B FI117136 B FI 117136B FI 951792 A FI951792 A FI 951792A FI 951792 A FI951792 A FI 951792A FI 117136 B FI117136 B FI 117136B
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- Prior art keywords
- erythropoietin
- epo
- analog
- pro
- human
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- 229940052586 pro 12 Drugs 0.000 description 1
- 108010077112 prolyl-proline Proteins 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000000754 repressing effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
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- 230000007017 scission Effects 0.000 description 1
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- 108010026333 seryl-proline Proteins 0.000 description 1
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- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000013222 sprague-dawley male rat Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Diabetes (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (11)
1. Förfarande for framställning av en terä] användbar analog av humant erytropoietin, varvid 5 (a) nämnda analog innehäller ätminstone e1 satt N-glykosylationsställe i en asparaginrest at] vid en av aminosyrapositionerna 30, 51, 57, 88, och 138 och atminstone en tillsatt N-kopplad kolhy ja har bundits till vilken som heist av nämnda am 10 positioner, eller (b) nämnda analog innehäller en förlängnir eller flera aminosyror i karboxylterminalen av ery tin, vilken förlängning innehäller ätminstone ett < lationsställe, eller 15 (c) nämnda analog omfattar avlagsning av e- flera glykosylationsställen för humant erytropoie en lika stor tillsats av icke-naturliga glykosyl. tallen, varvid kolhydratkedjornas positioner ändra; (d) nämnda analog innehäller ett extra < 20 sylationsställe vid aminosyrapositionen 123 och € O-kopplad kolhydratkedja har bundits tili nämnda < ,·. : raposition, • ·« kännetecknad av odling av en däggdji • ♦ · som omfattar en DNA-sekvens som kodar för nämndc • · • a • . 25 för humant erytropoietin, och den sälunda erhälln< gen isoleras. • · · *·· · 2. Förfarande enligt patentkrav 1, kannet • · · : av att analogen för humant erytropoietin är: Asn30Thr32Val87Asn88Thr90EPO. 30 3. Förfarande enligt patentkrav 1, kannet !**’: av att analoaen för humant ervtroDoietin väli s bl; Ser87Asn88Thr90 EPO; Ser87Asn88Gly89Thr90 EPO; SerB7AsnseThr90Thr92 EPO; Ser87Asn88Thr90Ala162 EPO;
5 Asn69Thr71Ser87Asn88Thr90 EPO; Asn89Ile90Thr91 EPO; Ser87Asn89Ile90Thr91 EPO; Asn135Thr138 EPO; och Asn138Thr140 EPO. 10 4. Förfarande enligt patentkrav 1, kännet av att förlängningen omfattar ett peptidfragment letts frän karboxiterminalen för humant koriongc pin.
5. Förfarande enligt patentkrav 4, kännet 15 av att analogen väljs bland följande: (a) humant erytropoietin med aminosyras Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Se Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pr som tillägg tili karboxiterminalen; 20 (b) analogen enligt punkt (a) vilken dess fattar Ser87Asn88Thr90 EPO; och : (c) analogen enligt punkt (a) vilken dess .·:·[ fattar Asn30Thr32Val87Asn88Thr90 EPO. « «
6. Förfarande enligt patentkrav 1, kännet * 4* * . 25 av att det icke-naturliga glykosylationsstället ä kopplat kolhydratställe vid aminosyrapositionen 88 4 * * * mant erytropoietin. : 7. Förfarande enligt patentkrav 6, kännet av att analogen väljs bland följande: : 30 Gln24Ser87Asn88Thr90 EPO; ***** n38Qar07aQnS8Thr9() TT DA * 85 1 1
9. DNA-sekvens, kännetecknad av att d för en analog för humant erytropoietin enligt nägo tentkraven 1-8.
10. Eukaryotisk värdcell, kännetecknad 5 den transfekterats med en DNA-sekvens enligt pate pä ett sätt som tillater att värdcellen uttrycker log.
11. Värdcell enligt patentkrav 10, kännet av att den är en CHO-cell. .\ : • ·· • · < I « • · ♦ ♦ * · • M * • · • * ♦ · · • * « · : ♦ ♦ ·.· * • « « • « · ··· ♦ · * ·
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10801693A | 1993-08-17 | 1993-08-17 | |
| US10801693 | 1993-08-17 | ||
| US9409257 | 1994-08-16 | ||
| PCT/US1994/009257 WO1995005465A1 (en) | 1993-08-17 | 1994-08-16 | Erythropoietin analogs |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI951792A0 FI951792A0 (sv) | 1995-04-13 |
| FI951792L FI951792L (sv) | 1995-05-18 |
| FI117136B true FI117136B (sv) | 2006-06-30 |
Family
ID=22319789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI951792A FI117136B (sv) | 1993-08-17 | 1995-04-13 | Förfarande för framställning av terapeutiskt användbara analoger av erytropoietin |
Country Status (26)
| Country | Link |
|---|---|
| EP (1) | EP0640619B2 (sv) |
| JP (3) | JP2938572B2 (sv) |
| KR (2) | KR100328769B1 (sv) |
| CN (1) | CN1057534C (sv) |
| AT (1) | ATE155796T1 (sv) |
| AU (1) | AU677097B2 (sv) |
| CA (1) | CA2147124C (sv) |
| CZ (3) | CZ291229B6 (sv) |
| DE (2) | DE69404401T3 (sv) |
| DK (1) | DK0640619T4 (sv) |
| ES (1) | ES2105442T5 (sv) |
| FI (1) | FI117136B (sv) |
| GR (1) | GR3024815T3 (sv) |
| HU (1) | HU220334B (sv) |
| IL (4) | IL192290A0 (sv) |
| LU (1) | LU90850I2 (sv) |
| LV (1) | LV10972B (sv) |
| NL (1) | NL300075I2 (sv) |
| NO (2) | NO323104B1 (sv) |
| NZ (1) | NZ273134A (sv) |
| RU (1) | RU2159814C2 (sv) |
| SI (1) | SI0640619T2 (sv) |
| SK (1) | SK282003B6 (sv) |
| UA (1) | UA49793C2 (sv) |
| WO (1) | WO1995005465A1 (sv) |
| ZA (1) | ZA946122B (sv) |
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| WO2022159414A1 (en) | 2021-01-22 | 2022-07-28 | University Of Rochester | Erythropoietin for gastroinfestinal dysfunction |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ210501A (en) * | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| US4954437A (en) * | 1986-09-15 | 1990-09-04 | Integrated Genetics, Inc. | Cell encoding recombinant human erythropoietin |
| AU646822B2 (en) * | 1989-10-13 | 1994-03-10 | Kirin-Amgen Inc. | Erythropoietin isoforms |
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1994
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2001
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