KR101229995B1 - 생체내 에리스로포이에틴 활성이 증진된 융합단백질 - Google Patents
생체내 에리스로포이에틴 활성이 증진된 융합단백질 Download PDFInfo
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- KR101229995B1 KR101229995B1 KR1020010072713A KR20010072713A KR101229995B1 KR 101229995 B1 KR101229995 B1 KR 101229995B1 KR 1020010072713 A KR1020010072713 A KR 1020010072713A KR 20010072713 A KR20010072713 A KR 20010072713A KR 101229995 B1 KR101229995 B1 KR 101229995B1
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- leu
- ectp
- fusion protein
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
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- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (5)
- 서열번호 2의 아미노산 서열로 이루어지는, 생체내에서 증진된 에리스로포이에틴(EPO) 활성을 갖는, 인간 EPO와 인간 융모성 성선자극호르몬(HCG) β 서브유닛 카르복시 말단 펩타이드(CTP)의 융합단백질.
- 삭제
- 삭제
- 제1항에 따른 융합단백질을 코딩하는 핵산.
- 제4항에 따른 핵산을 포함하는 재조합 벡터로 형질전환된 형질전환체를 배양하여 생체내 에리스로포이에틴 활성이 증진된 융합단백질을 제조하는 방법.
Priority Applications (7)
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JP2002549725A JP3946638B2 (ja) | 2000-12-11 | 2001-12-10 | 生体内エリスロポエチン活性が増強された融合蛋白質 |
PCT/KR2001/002137 WO2002048194A1 (en) | 2000-12-11 | 2001-12-10 | Fusion protein having the enhanced in vivo activity of erythropoietin |
EP01270554A EP1342730B1 (en) | 2000-12-11 | 2001-12-10 | Fusion protein having the enhanced in vivo activity of erythropoietin |
DE60117919T DE60117919T2 (de) | 2000-12-11 | 2001-12-10 | Fusionsprotein mit verbesserter in vivo erythropoietinwirkung |
US10/433,508 US8008454B2 (en) | 2000-12-11 | 2001-12-10 | Fusion protein having the enhanced in vivo activity of erythropoietin |
AU2002222740A AU2002222740A1 (en) | 2000-12-11 | 2001-12-10 | Fusion protein having the enhanced in vivo activity of erythropoietin |
AT01270554T ATE320449T1 (de) | 2000-12-11 | 2001-12-10 | Fusionsprotein mit verbesserter in vivo erythropoietinwirkung |
Applications Claiming Priority (2)
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KR1020000075230 | 2000-12-11 | ||
KR20000075230 | 2000-12-11 |
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KR20020046150A KR20020046150A (ko) | 2002-06-20 |
KR101229995B1 true KR101229995B1 (ko) | 2013-02-06 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1020010072713A KR101229995B1 (ko) | 2000-12-11 | 2001-11-21 | 생체내 에리스로포이에틴 활성이 증진된 융합단백질 |
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US (1) | US8008454B2 (ko) |
KR (1) | KR101229995B1 (ko) |
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US8048849B2 (en) | 2006-02-03 | 2011-11-01 | Modigene, Inc. | Long-acting polypeptides and methods of producing same |
US8450269B2 (en) | 2006-02-03 | 2013-05-28 | Prolor Biotech Ltd. | Long-acting growth hormone and methods of producing same |
US10221228B2 (en) | 2006-02-03 | 2019-03-05 | Opko Biologics Ltd. | Long-acting polypeptides and methods of producing and administering same |
US10351615B2 (en) | 2006-02-03 | 2019-07-16 | Opko Biologics Ltd. | Methods of treatment with long-acting growth hormone |
US9458444B2 (en) | 2006-02-03 | 2016-10-04 | Opko Biologics Ltd. | Long-acting coagulation factors and methods of producing same |
US7553941B2 (en) | 2006-02-03 | 2009-06-30 | Modigene Inc | Long-acting polypeptides and methods of producing same |
US8759292B2 (en) | 2006-02-03 | 2014-06-24 | Prolor Biotech, Llc | Long-acting coagulation factors and methods of producing same |
US20140113860A1 (en) | 2006-02-03 | 2014-04-24 | Prolor Biotech Ltd. | Long-acting polypeptides and methods of producing and administering same |
US20150038413A1 (en) | 2006-02-03 | 2015-02-05 | Opko Biologics Ltd. | Long-acting polypeptides and methods of producing and administering same |
US8946155B2 (en) | 2006-02-03 | 2015-02-03 | Opko Biologics Ltd. | Long-acting polypeptides and methods of producing and administering same |
US9249407B2 (en) | 2006-02-03 | 2016-02-02 | Opko Biologics Ltd. | Long-acting coagulation factors and methods of producing same |
US8304386B2 (en) * | 2006-02-03 | 2012-11-06 | Prolor Biotech, Inc. | Long-acting growth hormone and methods of producing same |
US8476234B2 (en) * | 2006-02-03 | 2013-07-02 | Prolor Biotech Inc. | Long-acting coagulation factors and methods of producing same |
US8048848B2 (en) | 2006-02-03 | 2011-11-01 | Prolor Biotech Ltd. | Long-acting interferons and derivatives thereof and methods thereof |
WO2009099763A1 (en) | 2008-01-30 | 2009-08-13 | Indiana University Research And Technology Corporation | Ester-based peptide prodrugs |
US9663778B2 (en) | 2009-07-09 | 2017-05-30 | OPKO Biologies Ltd. | Long-acting coagulation factors and methods of producing same |
JP5912112B2 (ja) | 2010-06-24 | 2016-04-27 | インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーションIndiana University Research And Technology Corporation | アミド系インスリンプロドラッグ |
EP2793932B1 (en) | 2011-12-20 | 2018-10-03 | Indiana University Research and Technology Corporation | Ctp-based insulin analogs for treatment of diabetes |
EP2838552A4 (en) | 2012-04-19 | 2016-05-18 | Opko Biolog Ltd | OXYNTOMODULIN VARIANTS WITH EXTENDED ACTION AND PROCESSES FOR PRODUCING SAME |
EP3395358B1 (en) | 2012-09-26 | 2019-11-06 | Indiana University Research and Technology Corporation | Insulin analog dimers |
WO2014080401A2 (en) * | 2012-11-20 | 2014-05-30 | Prolor Biotech Inc | Method of increasing the hydrodynamic volume of polypeptides by attaching to gonadotrophin carboxy terminal peptides |
CN103864913A (zh) | 2012-12-18 | 2014-06-18 | 联亚生技开发股份有限公司 | 重组蛋白 |
US20160024181A1 (en) | 2013-03-13 | 2016-01-28 | Moderna Therapeutics, Inc. | Long-lived polynucleotide molecules |
JP6538645B2 (ja) | 2013-03-14 | 2019-07-03 | インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーションIndiana University Research And Technology Corporation | インスリン‐インクレチン複合物 |
US20150158926A1 (en) | 2013-10-21 | 2015-06-11 | Opko Biologics, Ltd. | Long-acting polypeptides and methods of producing and administering same |
US10232020B2 (en) | 2014-09-24 | 2019-03-19 | Indiana University Research And Technology Corporation | Incretin-insulin conjugates |
WO2016049174A1 (en) | 2014-09-24 | 2016-03-31 | Indiana University Research And Technology Corporation | Lipidated amide-based insulin prodrugs |
CN113289009A (zh) | 2015-06-19 | 2021-08-24 | Opko生物科学有限公司 | 长效凝固因子及其产生方法 |
CN118085104A (zh) | 2016-07-11 | 2024-05-28 | Opko生物科学有限公司 | 长效凝血因子及其制备方法 |
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EP0640619A1 (en) * | 1993-08-17 | 1995-03-01 | Amgen Inc. | Erythropoietin analogs with additional glycosylation sites |
US5585345A (en) * | 1989-02-21 | 1996-12-17 | Washington University | CTP extended form of glycoprotein hormones |
US5792460A (en) * | 1989-02-21 | 1998-08-11 | Washington University | Modified glycoprotein hormones having a CTP at the amino terminus |
WO2001002017A2 (en) * | 1999-07-02 | 2001-01-11 | F. Hoffmann-La Roche Ag | Erythropoietin conjugates with polyethylenglycol |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6225449B1 (en) | 1991-10-04 | 2001-05-01 | Washington University | Hormone analogs with multiple CTP extensions |
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2001
- 2001-11-21 KR KR1020010072713A patent/KR101229995B1/ko not_active IP Right Cessation
- 2001-12-10 US US10/433,508 patent/US8008454B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5585345A (en) * | 1989-02-21 | 1996-12-17 | Washington University | CTP extended form of glycoprotein hormones |
US5712122A (en) * | 1989-02-21 | 1998-01-27 | Washington University | Carboxy terminal peptide-extended proteins |
US5792460A (en) * | 1989-02-21 | 1998-08-11 | Washington University | Modified glycoprotein hormones having a CTP at the amino terminus |
EP0640619A1 (en) * | 1993-08-17 | 1995-03-01 | Amgen Inc. | Erythropoietin analogs with additional glycosylation sites |
WO2001002017A2 (en) * | 1999-07-02 | 2001-01-11 | F. Hoffmann-La Roche Ag | Erythropoietin conjugates with polyethylenglycol |
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KR20020046150A (ko) | 2002-06-20 |
US8008454B2 (en) | 2011-08-30 |
US20090221037A1 (en) | 2009-09-03 |
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