DE3050722C2 - - Google Patents

Info

Publication number
DE3050722C2
DE3050722C2 DE3050722A DE3050722A DE3050722C2 DE 3050722 C2 DE3050722 C2 DE 3050722C2 DE 3050722 A DE3050722 A DE 3050722A DE 3050722 A DE3050722 A DE 3050722A DE 3050722 C2 DE3050722 C2 DE 3050722C2
Authority
DE
Germany
Prior art keywords
plasmid
dna
growth hormone
fragment
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE3050722A
Other languages
German (de)
English (en)
Inventor
David V. Goeddel
Herbert L. Burlingame Calif. Us Heyneker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26733870&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3050722(C2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DE3050722C2 publication Critical patent/DE3050722C2/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
DE3050722A 1979-07-05 1980-06-24 Expired DE3050722C2 (enExample)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/055,126 US4342832A (en) 1979-07-05 1979-07-05 Method of constructing a replicable cloning vehicle having quasi-synthetic genes

Publications (1)

Publication Number Publication Date
DE3050722C2 true DE3050722C2 (enExample) 1987-09-24

Family

ID=26733870

Family Applications (3)

Application Number Title Priority Date Filing Date
DE3023627A Ceased DE3023627A1 (de) 1979-07-05 1980-06-24 Mikrobielle expression von quasi- synthetischen genen
DE3050725A Expired DE3050725C2 (enExample) 1979-07-05 1980-06-24
DE3050722A Expired DE3050722C2 (enExample) 1979-07-05 1980-06-24

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE3023627A Ceased DE3023627A1 (de) 1979-07-05 1980-06-24 Mikrobielle expression von quasi- synthetischen genen
DE3050725A Expired DE3050725C2 (enExample) 1979-07-05 1980-06-24

Country Status (42)

Country Link
US (7) US4342832A (enExample)
EP (1) EP0022242B1 (enExample)
JP (4) JPH0612996B2 (enExample)
KR (2) KR830003574A (enExample)
AR (1) AR244341A1 (enExample)
AT (1) ATE82324T1 (enExample)
AU (2) AU533697B2 (enExample)
BE (1) BE884012A (enExample)
BG (1) BG41135A3 (enExample)
BR (1) BR8008736A (enExample)
CA (2) CA1164375A (enExample)
CH (1) CH661939A5 (enExample)
CS (3) CS250652B2 (enExample)
DD (3) DD210071A5 (enExample)
DE (3) DE3023627A1 (enExample)
DK (2) DK173503B1 (enExample)
EG (1) EG14819A (enExample)
ES (2) ES8105386A1 (enExample)
FI (2) FI802030A7 (enExample)
FR (2) FR2460330B1 (enExample)
GB (2) GB2055382B (enExample)
GR (1) GR69320B (enExample)
HK (3) HK87384A (enExample)
IE (3) IE50462B1 (enExample)
IL (3) IL60312A (enExample)
IT (1) IT1131393B (enExample)
KE (3) KE3446A (enExample)
MX (1) MX172674B (enExample)
MY (3) MY8500764A (enExample)
NL (1) NL930114I2 (enExample)
NO (2) NO167673C (enExample)
NZ (2) NZ201312A (enExample)
OA (1) OA06562A (enExample)
PH (1) PH19814A (enExample)
PL (1) PL149278B1 (enExample)
PT (1) PT71487A (enExample)
RO (1) RO93374B (enExample)
SG (1) SG56984G (enExample)
WO (1) WO1981000114A1 (enExample)
YU (3) YU163580A (enExample)
ZA (1) ZA803600B (enExample)
ZW (1) ZW14180A1 (enExample)

Families Citing this family (192)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4898830A (en) * 1979-07-05 1990-02-06 Genentech, Inc. Human growth hormone DNA
US6455275B1 (en) 1980-02-25 2002-09-24 The Trustees Of Columbia University In The City Of New York DNA construct for producing proteinaceous materials in eucaryotic cells
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4711843A (en) * 1980-12-31 1987-12-08 Cetus Corporation Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US6610830B1 (en) 1980-07-01 2003-08-26 Hoffman-La Roche Inc. Microbial production of mature human leukocyte interferons
IE53166B1 (en) * 1980-08-05 1988-08-03 Searle & Co Synthetic urogastrone gene,corresponding plasmid recombinants,transformed cells,production thereof and urogastrone expression
US7101981B1 (en) 1980-08-26 2006-09-05 Regents Of The University Of California Bovine growth hormone recombinantly produced in E. coli
US4725549A (en) * 1980-09-22 1988-02-16 The Regents Of The University Of California Human and rat prolactin and preprolactin cloned genes
GR81946B (enExample) * 1980-09-25 1984-12-12 Genentech Inc
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
JPS57181098A (en) * 1981-04-30 1982-11-08 Japan Found Cancer Novel recombinant dna
US4801685A (en) * 1981-08-14 1989-01-31 Hoffmann-La Roche Inc. Microbial production of mature human leukocyte interferon K and L
US4810645A (en) * 1981-08-14 1989-03-07 Hoffmann-La Roche Inc. Microbial production of mature human leukocyte interferon K and L
NZ201918A (en) * 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US4880910A (en) * 1981-09-18 1989-11-14 Genentech, Inc. Terminal methionyl bovine growth hormone and its use
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4665160A (en) * 1982-03-22 1987-05-12 Genentech, Inc. Novel human growth hormone like protein HGH-V encoded in the human genome
US4446235A (en) * 1982-03-22 1984-05-01 Genentech, Inc. Method for cloning human growth hormone varient genes
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
IE55244B1 (en) * 1982-05-25 1990-07-18 Lilly Co Eli Cloning vectors for expression of exogenous protein
US4778759A (en) * 1982-07-09 1988-10-18 Boyce, Thompson Institute For Plant Research, Inc. Genetic engineering in cyanobacteria
US4891315A (en) * 1982-10-25 1990-01-02 American Cyanamid Company Production of herpes simplex viral porteins
WO1984000775A1 (en) * 1982-08-10 1984-03-01 Univ Columbia The use of eucaryotic promoter sequences in the production of proteinaceous materials
US4530904A (en) * 1982-09-03 1985-07-23 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria
JPS59501852A (ja) * 1982-09-16 1984-11-08 アムジエン 鳥類の成長ホルモン
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
US4666839A (en) * 1982-12-01 1987-05-19 Amgen Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin
JPS59106297A (ja) * 1982-12-07 1984-06-19 Rikagaku Kenkyusho ヒト生長ホルモンのカルボキシ末端遺伝子の合成法
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
US5618697A (en) * 1982-12-10 1997-04-08 Novo Nordisk A/S Process for preparing a desired protein
US4634678A (en) * 1982-12-13 1987-01-06 Molecular Genetics Research And Development Limited Partnership Plasmid cloning and expression vectors for use in microorganisms
GB8303383D0 (en) * 1983-02-08 1983-03-16 Biogen Nv Sequences recombinant dna molecules
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
IL71991A (en) 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5242798A (en) * 1983-07-21 1993-09-07 Scripps Clinic And Research Foundation Synthetic polypeptides corresponding to portions of proteinoids translated from brain-specific mRNAs, receptors, methods and diagnostics using the same
US4900811A (en) * 1983-07-21 1990-02-13 Scripps Clinic And Research Foundation Synthetic polypeptides corresponding to portions of proteinoids translated from brain-specific mRNAs, receptors, methods and diagnostics using the same
JPS60137291A (ja) * 1983-12-26 1985-07-20 Takeda Chem Ind Ltd 発現ベクター
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
CA1272144A (en) * 1984-06-29 1990-07-31 Tamio Mizukami Fish growth hormone polypeptide
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
WO1986002068A1 (fr) * 1984-09-26 1986-04-10 Takeda Chemical Industries, Ltd. Separation mutuelle de proteines
US4680262A (en) * 1984-10-05 1987-07-14 Genentech, Inc. Periplasmic protein recovery
ATE78515T1 (de) * 1984-10-05 1992-08-15 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
NZ213759A (en) * 1984-10-19 1989-01-27 Genentech Inc Lhrh-ctp protein conjugates influencing prolactin fsh and lh release
US4645829A (en) * 1984-10-29 1987-02-24 Monsanto Company Method for separating polypeptides
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US4861868A (en) * 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
DE3683186D1 (de) 1985-04-25 1992-02-13 Hoffmann La Roche Rekombinantes humaninterleukin-1.
WO1987000201A1 (en) * 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
US5041381A (en) 1986-07-03 1991-08-20 Schering Corporation Monoclonal antibodies against human interleukin-4 and hybridomas producing the same
US4892764A (en) * 1985-11-26 1990-01-09 Loctite Corporation Fiber/resin composites, and method of making the same
US5827826A (en) * 1986-03-03 1998-10-27 Rhone-Poulenc Rorer Pharmaceuticals Inc. Compositions of human endothelial cell growth factor
US5552528A (en) * 1986-03-03 1996-09-03 Rhone-Poulenc Rorer Pharmaceuticals Inc. Bovine b-endothelial cell growth factor
DE3788592T2 (de) * 1986-05-07 1994-04-28 Eniricerche Spa Plasmidvektor zur Expression im Bazillus, dessen Umwendung für die Klonierung des menschlichen Wachstumshormongens und Verfahren zur Produktion des Hormons.
EP0252588A3 (en) * 1986-05-12 1989-07-12 Smithkline Beecham Corporation Process for the isolation and purification of p. falciparum cs protein expressed in recombinant e. coli, and its use as a vaccine
US4806239A (en) * 1986-11-28 1989-02-21 Envirotech Corporation Apparatus for shifting filter plates in a filter press
JPS63159244A (ja) * 1986-12-23 1988-07-02 三菱マテリアル株式会社 二層の押出成形珪酸質―石灰質系成形品の製造方法
ATE119573T1 (de) * 1986-12-31 1995-03-15 Lucky Ltd Verfahren zur herstellung eines lachswachstumshormons mittels eines kunstgens.
WO1988005082A1 (en) * 1987-01-07 1988-07-14 Allied Corporation Microbial production of peptide oligomers
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
HUT51901A (en) * 1987-06-22 1990-06-28 Genetics Inst Process for production of new trombolitic ensimes
FR2624835B2 (fr) * 1987-08-05 1990-09-07 Hassevelde Roger Support ergonomique a creme glacee, a cuillere integree, transformable en tout autre objet apres son utilisation premiere
US5268267A (en) * 1987-08-21 1993-12-07 The General Hospital Corporation Method for diagnosing small cell carcinoma
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
JPH0231042U (enExample) * 1988-08-19 1990-02-27
US5130422A (en) * 1988-08-29 1992-07-14 Monsanto Company Variant somatotropin-encoding DNA
EP0394409B1 (en) 1988-09-02 1997-06-11 The Rockefeller University Macrophage-derived inflammatory mediator (mip-2)
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
US5075227A (en) * 1989-03-07 1991-12-24 Zymogenetics, Inc. Directional cloning
US4960301A (en) * 1989-03-27 1990-10-02 Fry Steven A Disposable liner for pickup truck beds
US5164180A (en) 1989-05-18 1992-11-17 Mycogen Corporation Bacillus thuringiensis isolates active against lepidopteran pests
US5266477A (en) * 1990-02-02 1993-11-30 Pitman-Moore, Inc. Monoclonal antibodies which differentiate between native and modified porcine somatotropins
CA2041446A1 (en) 1990-05-15 1991-11-16 August J. Sick Bacillus thuringiensis genes encoding novel dipteran-active toxins
US5849694A (en) * 1990-07-16 1998-12-15 Synenki; Richard M. Stable and bioactive modified porcine somatotropin and pharmaceutical compositions thereof
US5744139A (en) * 1991-06-28 1998-04-28 University Of Tennessee Research Corporation Insulin-like growth factor I (IGF-1) induced improvement of depressed T4/T8 ratios
US5202119A (en) * 1991-06-28 1993-04-13 Genentech, Inc. Method of stimulating immune response
US5591709A (en) * 1991-08-30 1997-01-07 Life Medical Sciences, Inc. Compositions and methods for treating wounds
HUT67319A (en) * 1991-08-30 1995-03-28 Life Medical Sciences Inc Compositions for treating wounds
US5317012A (en) * 1991-10-04 1994-05-31 The University Of Tennessee Research Corporation Human growth hormone induced improvement in depressed T4/T8 ratio
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
WO1994008611A1 (en) * 1992-10-22 1994-04-28 Sloan-Kettering Institute For Cancer Research GROWTH HORMONE FRAGMENT hGH 108-129
DE69435280D1 (de) 1993-03-10 2010-04-15 Glaxosmithkline Llc Phosphodiesterase des menschlichen Gehirns und Screening-Verfahren
FR2738842B1 (fr) * 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
GB9526733D0 (en) * 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US5760187A (en) * 1996-02-22 1998-06-02 Mitsui Toatsu Chemicals, Inc. Purification process of a human growth hormone
JP3794748B2 (ja) * 1996-03-04 2006-07-12 第一アスビオファーマ株式会社 メタノール代謝系を有する微生物の培養法
WO1998007830A2 (en) * 1996-08-22 1998-02-26 The Institute For Genomic Research COMPLETE GENOME SEQUENCE OF THE METHANOGENIC ARCHAEON, $i(METHANOCOCCUS JANNASCHII)
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
JP2001510989A (ja) * 1996-11-01 2001-08-07 スミスクライン・ビーチャム・コーポレイション 新規コーディング配列
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
EP1749834B1 (en) 1997-12-18 2012-04-25 Monsanto Technology LLC Insect-resistant transgenic plants and methods for improving delta-endotoxin activity against target insects
US6087128A (en) 1998-02-12 2000-07-11 Ndsu Research Foundation DNA encoding an avian E. coli iss
EP1066328B1 (en) 1998-03-31 2008-09-17 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production
US6271444B1 (en) 1998-07-10 2001-08-07 Calgene Llc Enhancer elements for increased translation in plant plastids
US6512162B2 (en) 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
ATE481637T1 (de) 1998-08-28 2010-10-15 Febit Holding Gmbh Verfahren zur herstellung von biochemischen reaktionsträgern
WO2000049142A1 (de) 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
US6946265B1 (en) 1999-05-12 2005-09-20 Xencor, Inc. Nucleic acids and proteins with growth hormone activity
US6187750B1 (en) 1999-08-25 2001-02-13 Everyoung Technologies, Inc. Method of hormone treatment for patients with symptoms consistent with multiple sclerosis
AT408721B (de) 1999-10-01 2002-02-25 Cistem Biotechnologies Gmbh Pharmazeutische zusammensetzung enthaltend ein antigen
GB9924351D0 (en) * 1999-10-14 1999-12-15 Brennan Frank Immunomodulation methods and compositions
US6562790B2 (en) 2000-02-05 2003-05-13 Chein Edmund Y M Hormone therapy methods and hormone products for abating coronary artery blockage
EP1278767A4 (en) 2000-04-12 2003-11-12 Principia Pharmaceutical Corp ALBUMIN FUSED PROTEINS
US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
WO2002043709A1 (fr) 2000-12-01 2002-06-06 Takeda Chemical Industries, Ltd. Procede de production d'une preparation contenant une substance bioactive
US20030049731A1 (en) * 2000-12-05 2003-03-13 Bowdish Katherine S. Engineered plasmids and their use for in situ production of genes
US20020164712A1 (en) * 2000-12-11 2002-11-07 Tonghua Gantech Biotechnology Ltd. Chimeric protein containing an intramolecular chaperone-like sequence
CA2446739A1 (en) * 2001-05-25 2002-12-05 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
US6720538B2 (en) * 2001-06-18 2004-04-13 Homedics, Inc. Thermostat variation compensating knob
ES2556338T3 (es) 2001-10-10 2016-01-15 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
US20030171285A1 (en) * 2001-11-20 2003-09-11 Finn Rory F. Chemically-modified human growth hormone conjugates
AU2002364587A1 (en) 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
EP2277889B1 (en) 2001-12-21 2014-07-09 Human Genome Sciences, Inc. Fusion proteins of albumin and interferon beta
WO2004022593A2 (en) * 2002-09-09 2004-03-18 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
TWI281864B (en) * 2002-11-20 2007-06-01 Pharmacia Corp N-terminally monopegylated human growth hormone conjugates and process for their preparation
RU2233879C1 (ru) * 2002-12-17 2004-08-10 Институт биоорганической химии им. академиков М.М.Шемякина и Ю.А.Овчинникова РАН Рекомбинантная плазмидная днк pes1-6, кодирующая полипептид соматотропин, и штамм escherichia coli bl 21(de3)/pes1-6-продуцент рекомбинантного соматотропина
BR0317888A (pt) 2002-12-31 2005-12-06 Altus Pharmaceuticals Inc Cristais do hormÈnio do crescimento humano e processos para preparação dos mesmos
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2004074345A2 (en) * 2003-02-19 2004-09-02 Pharmacia Corporation Carbonate esters of polyethylene glycol activated by means of oxalate esters
MXPA05011965A (es) * 2003-05-09 2006-02-02 Pharmexa As Analogos inmunogenicos de tnf-alfa humano con citotoxicidad reducida y metodos para su preparacion.
BRPI0507169A (pt) * 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos
JP2008503233A (ja) * 2004-06-23 2008-02-07 ユーエスブイ・リミテッド 胎盤および下垂体アイソフォームに由来するキメラヒト成長ホルモンおよび該キメラを得るための方法
US20060024288A1 (en) * 2004-08-02 2006-02-02 Pfizer Inc. tRNA synthetase fragments
US8282921B2 (en) * 2004-08-02 2012-10-09 Paul Glidden tRNA synthetase fragments
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
BRPI0518661A2 (pt) * 2004-12-22 2008-12-02 Ambrx Inc mÉtodos para expressço e purificaÇço do hormânio do crescimento humano recombinante
AU2005322019B2 (en) 2004-12-22 2010-08-26 Ambrx, Inc. Formulations of human growth hormone comprising a non-naturally encoded amino acid
NZ584597A (en) 2004-12-22 2011-09-30 Ambrx Inc Modified human growth hormone
EP1871795A4 (en) 2005-04-08 2010-03-31 Biogenerix Ag COMPOSITIONS AND METHOD FOR PRODUCING GLYCOSYLATION MUTANTS OF A PROTEASE-RESISTANT HUMAN GROWTH HORMONE
US7576053B2 (en) 2005-06-13 2009-08-18 Rigel Pharmaceuticals, Inc. Methods and compositions for treating degenerative bone disorders
US8030271B2 (en) * 2005-07-05 2011-10-04 Emisphere Technologies, Inc. Compositions and methods for buccal delivery of human growth hormone
EP1928504B1 (en) * 2005-08-19 2013-04-24 University of Technology, Sydney Liver-directed gene therapy
CN106443006A (zh) 2005-11-16 2017-02-22 Ambrx公司 包括非天然氨基酸的方法和组合物
US20090023629A1 (en) * 2005-12-23 2009-01-22 Altus Pharmaceuticals Inc. Compositions comprising polycation-complexed protein crystals and methods of treatment using them
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
EP2120869A2 (en) 2006-12-18 2009-11-25 Altus Pharmaceuticals Inc. Human growth hormone formulations
US20080260820A1 (en) * 2007-04-19 2008-10-23 Gilles Borrelly Oral dosage formulations of protease-resistant polypeptides
JP2010524508A (ja) * 2007-04-27 2010-07-22 ダウ グローバル テクノロジーズ インコーポレイティド 可溶性組換え二十面体ウイルス様粒子の生産及びインビボ組織化の改善
AU2007352618B2 (en) 2007-05-02 2013-07-18 Boehringer Ingelheim Animal Health USA Inc. DNA plasmids having improved expression and stability
WO2009012502A1 (en) * 2007-07-19 2009-01-22 The Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Self- anchoring mems intrafascicular neural electrode
US7939447B2 (en) * 2007-10-26 2011-05-10 Asm America, Inc. Inhibitors for selective deposition of silicon containing films
CA2712606A1 (en) 2008-02-08 2009-08-13 Ambrx, Inc. Modified leptin polypeptides and their uses
HUE029926T2 (en) 2008-04-29 2017-04-28 Ascendis Pharma Growth Disorders Div As Pegylated recombinant human growth hormone compounds
CN103255130B (zh) 2008-04-30 2015-12-23 斯特莱科生物有限公司 高纯度质粒dna制备物及其制备方法
CN102119033B (zh) * 2008-06-25 2015-04-08 布拉施生物技术有限责任公司 用于增强促生长素抑制素免疫原性的组合物和方法
DK2303314T3 (da) * 2008-06-25 2014-01-13 Braasch Biotech Llc Chloramphenicol-acetyltransferase (cat)-defekt somatostatin-fusionsprotein og anvendelser deraf til kvæg
US8703717B2 (en) * 2009-02-03 2014-04-22 Amunix Operating Inc. Growth hormone polypeptides and methods of making and using same
KR101544245B1 (ko) 2009-02-03 2015-08-12 아뮤닉스 오퍼레이팅 인코포레이티드 연장된 재조합 폴리펩티드 및 이를 포함하는 조성물
WO2010096394A2 (en) 2009-02-17 2010-08-26 Redwood Biosciences, Inc. Aldehyde-tagged protein-based drug carriers and methods of use
WO2010094772A1 (en) 2009-02-20 2010-08-26 Febit Holding Gmbh Synthesis of sequence-verified nucleic acids
US9849188B2 (en) 2009-06-08 2017-12-26 Amunix Operating Inc. Growth hormone polypeptides and methods of making and using same
JP5813641B2 (ja) 2009-08-24 2015-11-17 アムニクス オペレーティング インコーポレイテッド 凝固第ｉｘ因子組成物ならびにそれを製造および使用する方法
JP2013513647A (ja) 2009-12-15 2013-04-22 アセンディス ファーマ エー／エス ポリマー担体に一時的に連結された乾燥成長ホルモン組成物
EP2516455A4 (en) 2009-12-21 2013-05-22 Ambrx Inc MODIFIED SWINE SOMATOTROPIN POLYPEPTIDES AND THEIR USES
EA201290541A1 (ru) 2009-12-21 2013-05-30 Амбркс, Инк. Модифицированные бычьи соматотропиновые полипептиды и их применение
WO2011103325A1 (en) 2010-02-17 2011-08-25 Elona Biotechnologies Methods for preparing human growth hormone
EP2446898A1 (en) 2010-09-30 2012-05-02 Laboratorios Del. Dr. Esteve, S.A. Use of growth hormone to enhance the immune response in immunosuppressed patients
US9540438B2 (en) 2011-01-14 2017-01-10 Redwood Bioscience, Inc. Aldehyde-tagged immunoglobulin polypeptides and methods of use thereof
RU2473556C1 (ru) * 2011-07-14 2013-01-27 Закрытое акционерное общество "ГЕНЕРИУМ" Промышленный способ получения и очистки рекомбинантного гормона роста человека из телец включения
BR112014019431A8 (pt) 2012-02-07 2017-07-11 Global Bio Therapeutics Usa Inc Método compartimentalizado de distribuição de ácido nucleico e composições e usos desses
EP3564260B1 (en) 2012-02-15 2022-10-19 Bioverativ Therapeutics Inc. Factor viii compositions and methods of making and using same
SI2822577T1 (sl) 2012-02-15 2019-07-31 Bioverativ Therapeutics Inc. Rekombinantni proteini faktorja VIII
US8809259B2 (en) 2012-03-26 2014-08-19 Pronutria, Inc. Charged nutritive proteins and methods
CA2868469A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
AU2013240184B2 (en) 2012-03-26 2017-05-25 Axcella Health Inc. Nutritive fragments, proteins and methods
WO2013148328A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive proteins and methods
US9457096B2 (en) 2012-07-06 2016-10-04 Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Concet) Protozoan variant-specific surface proteins (VSP) as carriers for oral drug delivery
JP6297692B2 (ja) 2013-08-08 2018-03-20 グローバル・バイオ・セラピューティクス・インコーポレイテッドＧｌｏｂａｌ Ｂｉｏ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 低侵襲処置用クランプデバイスおよびその使用
WO2015023891A2 (en) 2013-08-14 2015-02-19 Biogen Idec Ma Inc. Factor viii-xten fusions and uses thereof
US20160354436A1 (en) 2013-09-25 2016-12-08 Pronutria Biosciences, Inc. Compositions and Formulations for Increasing Renal Function and Treatment and Prevention of Renal Diseases, and Methods of Production and Use Thereof
KR102608645B1 (ko) 2014-11-18 2023-12-01 아센디스 파마 엔도크리놀로지 디비전 에이/에스 신규한 중합체성 hGH 프로드러그
PL3220892T3 (pl) 2014-11-21 2022-01-31 Ascendis Pharma Endocrinology Division A/S Postacie dawkowane długo działającego hormonu wzrostu
JP6909203B2 (ja) 2015-08-03 2021-07-28 バイオベラティブ セラピューティクス インコーポレイテッド 第ｉｘ因子融合タンパク質及びそれらの製造方法及び使用方法
CA3019398A1 (en) 2016-04-26 2017-11-02 R.P. Scherer Technologies, Llc Antibody conjugates and methods of making and using the same
AU2017366709A1 (en) 2016-12-02 2019-07-18 Bioverativ Therapeutics Inc. Methods of treating hemophilic arthropathy using chimeric clotting factors
TW202015723A (zh) 2018-05-18 2020-05-01 美商百歐維拉提夫治療公司 治療ａ型血友病的方法
WO2020004368A1 (ja) 2018-06-25 2020-01-02 Ｊｃｒファーマ株式会社 蛋白質含有水性液剤
CN109486847B (zh) * 2018-12-17 2021-03-02 江南大学 基于人工串联启动子的枯草芽孢杆菌高效诱导表达系统
EP3934625A1 (en) 2019-03-04 2022-01-12 Ascendis Pharma Endocrinology Division A/S Long-acting growth hormone dosage forms with superior efficacy to daily somatropin
WO2024175778A2 (en) 2023-02-23 2024-08-29 Micropep Technologies S.A. Antifungal peptides to control plant pathogens

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2007676A (en) * 1977-11-08 1979-05-23 Genentech Inc Method and means for microbial polypeptide expression
GB2007670A (en) * 1977-11-08 1979-05-23 Genentech Inc Triester process for the synthesis of oligonucleotides
GB2007675A (en) * 1977-11-08 1979-05-23 Genentech Inc Synthetic dna and process therefor
GB2008123A (en) * 1977-11-08 1979-05-31 Genentech Inc Method of means for microbial polypeptide expression

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3853832A (en) * 1971-04-27 1974-12-10 Harmone Res Foundation Synthetic human pituitary growth hormone and method of producing it
US3853833A (en) * 1971-04-27 1974-12-10 Hormone Res Foundation Synthetic human growth-promoting and lactogenic hormones and method of producing same
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
NL7607683A (nl) * 1976-07-12 1978-01-16 Akzo Nv Werkwijze ter bereiding van nieuwe peptiden en peptide-derivaten en de toepassing hiervan.
US4190495A (en) * 1976-09-27 1980-02-26 Research Corporation Modified microorganisms and method of preparing and using same
US4363877B1 (en) * 1977-09-23 1998-05-26 Univ California Recombinant dna transfer vectors
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
CH630089A5 (de) * 1977-09-09 1982-05-28 Ciba Geigy Ag Verfahren zur herstellung von siliciummodifizierten imidyl-phthalsaeurederivaten.
JPS5449837A (en) * 1977-09-19 1979-04-19 Kobashi Kogyo Kk Safety apparatus of soil block making machine
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
US4407948A (en) * 1977-09-23 1983-10-04 The Regents Of The University Of California Purification of nucleotide sequences suitable for expression in bacteria
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4356270A (en) * 1977-11-08 1982-10-26 Genentech, Inc. Recombinant DNA cloning vehicle
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
GR71912B (enExample) * 1978-08-11 1983-08-16 Univ California
EP0009930B2 (en) * 1978-10-10 1988-08-24 The Board Of Trustees Of The Leland Stanford Junior University Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it
US4332892A (en) * 1979-01-15 1982-06-01 President And Fellows Of Harvard College Protein synthesis
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
US4898830A (en) * 1979-07-05 1990-02-06 Genentech, Inc. Human growth hormone DNA
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
GR70279B (enExample) * 1979-09-12 1982-09-03 Univ California
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
GR79124B (enExample) * 1982-12-22 1984-10-02 Genentech Inc
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2007676A (en) * 1977-11-08 1979-05-23 Genentech Inc Method and means for microbial polypeptide expression
GB2007670A (en) * 1977-11-08 1979-05-23 Genentech Inc Triester process for the synthesis of oligonucleotides
GB2007675A (en) * 1977-11-08 1979-05-23 Genentech Inc Synthetic dna and process therefor
GB2008123A (en) * 1977-11-08 1979-05-31 Genentech Inc Method of means for microbial polypeptide expression

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
Cell, Vol. 13, 65-71, 1978 *
Cold Spring Harbor Smyposium, 43, 70, 1978 *
CRC Critical Reviews in Biochemistry, 123, Nov. 1976 *
Gene, 2, 1977, 95-113 *
J.Biol.Chem. 253, 2483, 1978 *
J.Mol.Biol., 1969, 41, 459-472 *
KORNBERG: DNA-Synthesis, 1974, 87-88 *
LEWIN: Gene Expression 1, 2, 1974, u. 3, 1977 *
Miles International Symposium Series, 10, 1977 *
MILLER: Experiments in Molecular Genetics, 1972 *
Molecular Cloniug of Recombinaut DNA, 99-177, 1977 *
Nature, 275, 617, 1978 u. 276, 795, 1978 *
Proc.Mat.Acad.Sci., USA 65, 168, 1970, USA 72, 3961, 1975, USA 74, 560, 1977, USA 75, 3727, 1978, USA 75, 4268, 1978, USA 75, 5765, 1978, USA 76, 106, 1979 *
Richtlinien für rekombinante DNA des National Institute of Health, 43 Fed.Reg. 60, 080, 1978 *
Science, 198, 1056, 1977 u. 196, 1313, 1977 *

Also Published As

Publication number Publication date
DE3050725C2 (enExample) 1988-03-10
GR69320B (enExample) 1982-05-14
JPS5621596A (en) 1981-02-28
RO93374A (ro) 1988-01-30
KR870000701B1 (ko) 1987-04-07
DD157343A5 (de) 1982-11-03
US4634677A (en) 1987-01-06
DK97381A (da) 1981-03-04
US4604359A (en) 1986-08-05
US4658021A (en) 1987-04-14
PT71487A (en) 1980-08-01
NO810608L (no) 1981-02-20
IE842193L (en) 1981-01-05
DD210071A5 (de) 1984-05-30
YU121183A (en) 1984-02-29
EP0022242A3 (en) 1982-04-07
ES493149A0 (es) 1981-05-16
GB2121047B (en) 1984-06-06
NO167674B (no) 1991-08-19
YU121283A (en) 1984-02-29
NO860680L (no) 1981-02-20
MY8500764A (en) 1985-12-31
IL69492A (en) 1985-04-30
RO93374B (ro) 1988-01-31
JPH05268970A (ja) 1993-10-19
JPH0612996B2 (ja) 1994-02-23
NL930114I2 (nl) 1994-10-17
IE50462B1 (en) 1986-04-30
GB2055382A (en) 1981-03-04
CS270884A2 (en) 1987-07-16
CA1164375A (en) 1984-03-27
NO167673C (no) 1991-11-27
ES8105386A1 (es) 1981-05-16
GB2121047A (en) 1983-12-14
JPH02478A (ja) 1990-01-05
CS250652B2 (en) 1987-05-14
DE3023627A1 (de) 1981-01-22
FI850198A7 (fi) 1985-01-16
GB2055382B (en) 1983-10-19
DK172132B1 (da) 1997-11-24
FR2460330B1 (fr) 1985-07-19
AU574351B2 (en) 1988-07-07
IL60312A0 (en) 1980-09-16
PH19814A (en) 1986-07-08
WO1981000114A1 (en) 1981-01-22
ES8205265A1 (es) 1982-06-01
IE50461B1 (en) 1986-04-30
BR8008736A (pt) 1981-04-28
CH661939A5 (de) 1987-08-31
FI850198A0 (fi) 1985-01-16
CS250655B2 (en) 1987-05-14
US4342832A (en) 1982-08-03
NO167674C (no) 1991-11-27
AR244341A1 (es) 1993-10-29
NO167673B (no) 1991-08-19
AU5949880A (en) 1981-01-15
MY8500763A (en) 1985-12-31
NL930114I1 (nl) 1993-10-18
US5424199A (en) 1995-06-13
FR2518572A1 (fr) 1983-06-24
YU163580A (en) 1984-02-29
US4601980A (en) 1986-07-22
NZ194043A (en) 1983-09-30
ATE82324T1 (de) 1992-11-15
IL69492A0 (en) 1983-11-30
EP0022242A2 (en) 1981-01-14
JPH0648987B2 (ja) 1994-06-29
IT1131393B (it) 1986-06-18
IE801214L (en) 1981-01-05
AU2583484A (en) 1984-07-12
MX172674B (es) 1994-01-06
BG41135A3 (bg) 1987-04-15
KR830003574A (ko) 1983-06-21
DK251490D0 (da) 1990-10-18
JPH08242881A (ja) 1996-09-24
OA06562A (fr) 1981-07-31
FI850198L (fi) 1985-01-16
IT8023070A0 (it) 1980-06-26
HK87384A (en) 1984-11-16
IL60312A (en) 1985-04-30
CS254973B2 (en) 1988-02-15
FR2460330A1 (fr) 1981-01-23
EG14819A (en) 1985-03-31
ZA803600B (en) 1981-07-29
DK173503B1 (da) 2001-01-15
FI802030A7 (fi) 1981-01-06
IE50460B1 (en) 1986-04-30
ZW14180A1 (en) 1980-09-10
EP0022242B1 (en) 1992-11-11
SG56984G (en) 1985-03-08
US5795745A (en) 1998-08-18
HK87484A (en) 1984-11-16
PL225376A1 (en) 1983-07-18
NZ201312A (en) 1983-09-30
ES499043A0 (es) 1982-06-01
DK251490A (da) 1990-10-18
DD210070A5 (de) 1984-05-30
AU1838883A (en) 1984-01-05
FR2518572B1 (fr) 1985-08-23
JP2622479B2 (ja) 1997-06-18
MY8500765A (en) 1985-12-31
AU533697B2 (en) 1983-12-08
KE3450A (en) 1984-10-05
CA1202256A (en) 1986-03-25
PL149278B1 (en) 1990-01-31
BE884012A (fr) 1980-12-29
KE3446A (en) 1984-10-05
HK87584A (en) 1984-11-16
KE3451A (en) 1984-10-05

Similar Documents

Publication Publication Date Title
DE3050722C2 (enExample)
DE3111405C2 (enExample)
DE3486216T2 (de) Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
DE3280422T2 (de) Verfahren und produkte zur leichten mikrobiologischen expression von dns-sequenzen.
EP0513073B1 (de) VERFAHREN ZUR ENZYMATISCHEN SPALTUNG REKOMBINANTER PROTEINE UNTER VERWENDUNG VON IgA-PROTEASEN
DE2848053A1 (de) Synthetische dna und verfahren zu ihrer herstellung
DE2848052A1 (de) Synthetische dna und verfahren zu ihrer herstellung
DE3752063T2 (de) Funktioneller DNS-Block und für Hirudin kodierendes Plasmid, transformierte Hefe aud Verfahren zur Herstellung von Hirudin.
DE2848051A1 (de) Synthetische dna und verfahren zu ihrer herstellung
DD254211A5 (de) Verfahren zur herstellung von plypeptiden
DD204711A5 (de) Verfahren zur herstellung eines dns-uebertragungsvektors
EP0453969B1 (de) Verfahren zur Herstellung von Fremdproteinen in Streptomyceten
DD201693A5 (de) Verfahren zur synthetisierung von menschlichem proinsulin
DE69011183T2 (de) Hefestämme für die Herstellung von heterologen reifen Proteinen, besonders von Hirudin.
DE3751081T2 (de) [Leu 13] Motilin, dessen kodierende DNS-Moleküle und Verfahren zu dessen Herstellung.
DE3485923T2 (de) Dna-gen, verfahren zu seiner herstellung und dieses gen enthaltendes plasmid.
DE3587205T2 (de) Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle.
DD210466A5 (de) Verfahren zur herstellung eines rekombinanten plasmids
EP0393039B1 (en) Process for producing peptides by specific cleavage of fusion proteins with collagenases obtained by genetic engineering
DE3587730T2 (de) Kontrollsysteme für rekombinant-manipulationen.
DE69213282T2 (de) Antiviren-Protein aus Mirabilis jalapa
EP0198415B1 (de) Veränderung der DNA-Sequenz zwischen Shine-Dalgarno-Sequenz und Startcodon des trp-Operons zur Steigerung der Proteinexpression
DE3336586A1 (de) Neuer expressionsvektor
DE69220306T2 (de) Faktor Xa-Linker DNA
DE3636117A1 (de) Verfahren zur expression von genen in hefe und dna-fragmente und plasmide zur verwendung bei diesem verfahren

Legal Events

Date Code Title Description
Q172 Divided out of (supplement):

Ref country code: DE

Ref document number: 3023627

8110 Request for examination paragraph 44
AC Divided out of

Ref country code: DE

Ref document number: 3023627

Format of ref document f/p: P

D2 Grant after examination
8364 No opposition during term of opposition