GB1521032A - Biological treatment - Google Patents

Biological treatment

Info

Publication number
GB1521032A
GB1521032A GB34994/74A GB3499474A GB1521032A GB 1521032 A GB1521032 A GB 1521032A GB 34994/74 A GB34994/74 A GB 34994/74A GB 3499474 A GB3499474 A GB 3499474A GB 1521032 A GB1521032 A GB 1521032A
Authority
GB
United Kingdom
Prior art keywords
dna
production
desired polypeptide
microorganism
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB34994/74A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Chemical Industries Ltd
Original Assignee
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Chemical Industries Ltd filed Critical Imperial Chemical Industries Ltd
Priority to GB34994/74A priority Critical patent/GB1521032A/en
Priority to SE7508905A priority patent/SE7508905L/en
Priority to FR7524749A priority patent/FR2281426A1/en
Priority to JP50096573A priority patent/JPS51125782A/en
Priority to CH1039175A priority patent/CH621147A5/en
Priority to DK360175A priority patent/DK360175A/en
Priority to DE2535554A priority patent/DE2535554C2/en
Publication of GB1521032A publication Critical patent/GB1521032A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Abstract

A cell culture which produces a desired polypeptide is prepared by genetic modification of microorganisms. The process comprises the following steps: a) splitting up molecules of a DNA receptor material obtained from a plasmid which is suitable for the infection of cells of the said microorganism, by treatment of the plasmid DNA with an endonuclease. b) Splitting up another genetic material which contains DNA donors and codes for the synthesis of a desired polypeptide, using a similar endonuclease, resulting in DNA fragments whose ends are compatible with those obtained according to a), c) mixing the DNA fragments obtained from the various sources, resulting in a random attachment of the DNA fragments under the influence of a DNA ligase, which results in hybrid DNA molecules which contain the combined fragments from both DNA sources, d) infecting the microorganism cells to be modified by contacting with the hybrid DNA molecules, e) allowing the infected microorganism to metabolise in the presence of a substrate which is suitable for conversion into the desired polypeptide and f) screening for production of the desired polypeptide and preparation of a cell culture which produces the polypeptide. Preferred applications are the production of mammalian polypeptides and proteins, the production of bacteria by "foreign" enzymes and secondary metabolites, e.g. glucose isomerase, and the production of compounds such as citric acid and cephalosporin by modified tissue.
GB34994/74A 1974-08-08 1974-08-08 Biological treatment Expired GB1521032A (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
GB34994/74A GB1521032A (en) 1974-08-08 1974-08-08 Biological treatment
SE7508905A SE7508905L (en) 1974-08-08 1975-08-07 WAY TO GENETICALLY MODIFY A MICROORGANISM.
FR7524749A FR2281426A1 (en) 1974-08-08 1975-08-07 PROCESS FOR MODIFICATION OF DESOXY-RIBONUCLEIC ACID AND PRODUCTS OBTAINED
JP50096573A JPS51125782A (en) 1974-08-08 1975-08-08 Hereditary modification of microorganism
CH1039175A CH621147A5 (en) 1974-08-08 1975-08-08 Process for obtaining a cell culture which produces a desired polypeptide by genetic modification of microorganisms
DK360175A DK360175A (en) 1974-08-08 1975-08-08 HYBRID DNA MOLECULES PROCEDURE
DE2535554A DE2535554C2 (en) 1974-08-08 1975-08-08 Biological process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB34994/74A GB1521032A (en) 1974-08-08 1974-08-08 Biological treatment

Publications (1)

Publication Number Publication Date
GB1521032A true GB1521032A (en) 1978-08-09

Family

ID=10372550

Family Applications (1)

Application Number Title Priority Date Filing Date
GB34994/74A Expired GB1521032A (en) 1974-08-08 1974-08-08 Biological treatment

Country Status (7)

Country Link
JP (1) JPS51125782A (en)
CH (1) CH621147A5 (en)
DE (1) DE2535554C2 (en)
DK (1) DK360175A (en)
FR (1) FR2281426A1 (en)
GB (1) GB1521032A (en)
SE (1) SE7508905L (en)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2402704A1 (en) * 1977-09-08 1979-04-06 Ici Ltd Microbiological prodn. of single cell protein - using genetically modified ammonia fixing microorganisms
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
GB2133408A (en) * 1980-12-31 1984-07-25 Ilkka Palva Method for improving the production of proteins in bacillus
US4469791A (en) * 1980-02-15 1984-09-04 Cpc International Inc. Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US5221619A (en) * 1977-11-08 1993-06-22 Genentech, Inc. Method and means for microbial polypeptide expression
US5424199A (en) * 1979-07-05 1995-06-13 Genentech, Inc. Human growth hormone
US5583013A (en) * 1977-11-08 1996-12-10 Genentech, Inc. Method and means for microbial polypeptide expression
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
US6455275B1 (en) 1980-02-25 2002-09-24 The Trustees Of Columbia University In The City Of New York DNA construct for producing proteinaceous materials in eucaryotic cells
US6835557B1 (en) 1980-01-08 2004-12-28 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides
US7588755B1 (en) 1980-04-03 2009-09-15 Biogen Idec Ma Inc. DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon-like polypeptides

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU527165B2 (en) * 1977-02-08 1983-02-17 Genentech Inc. Method and means for microbial polypeptide expression; synthetic DNA and process therefor
US4171824A (en) * 1977-05-23 1979-10-23 Foster Edwin E Bicyle
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
ES474852A1 (en) * 1977-11-08 1979-12-01 Genentech Inc Plasmid for transforming bacterial host to render it capable of polypeptide expression.
GB2007675B (en) * 1977-11-08 1982-09-02 Genentech Inc Synthetic dna and process therefor
SU943282A1 (en) * 1979-07-13 1982-07-15 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Process for producing l-treonine
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
IL58308A (en) * 1978-10-23 1983-10-31 Upjohn Co Process for preparing glucose isomerase using a recombinant plasmid
FR2444713A1 (en) * 1978-12-18 1980-07-18 Pasteur Institut PROCESS FOR PRODUCING DNA COMPRISING THE GENOME OF HEPATITIS B VIRUS AND VECTOR COMPRISING SAME
JPS55131397A (en) * 1979-04-02 1980-10-13 Ajinomoto Co Inc Preparation of l-threonine by fermentation
JPS561890A (en) * 1979-06-15 1981-01-10 Ajinomoto Co Inc Preparation of l-phenylalanine by fermentation
JPS565099A (en) * 1979-06-25 1981-01-20 Ajinomoto Co Inc Production of l-histidine through fermentation process and microorganism used therefor
JPS5618596A (en) * 1979-07-23 1981-02-21 Ajinomoto Co Inc Production of l-lysine through fermentation process
IL59690A (en) 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
JPS56160997A (en) * 1980-05-16 1981-12-11 Ajinomoto Co Inc Preparation of l-lysine by fermenaition method
FR2512058A1 (en) * 1981-08-25 1983-03-04 Centre Nat Rech Scient Synthetic plasmids giving faster metal oxidn. - for extn. of copper, uranium and manganese from their ores

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2402704A1 (en) * 1977-09-08 1979-04-06 Ici Ltd Microbiological prodn. of single cell protein - using genetically modified ammonia fixing microorganisms
US5583013A (en) * 1977-11-08 1996-12-10 Genentech, Inc. Method and means for microbial polypeptide expression
US5221619A (en) * 1977-11-08 1993-06-22 Genentech, Inc. Method and means for microbial polypeptide expression
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US6297355B1 (en) 1978-12-22 2001-10-02 Biogen, Inc. Polypeptides displaying HBV antigenicity or hbv antigen specificity
US6270955B1 (en) 1978-12-22 2001-08-07 Biogen, Inc. Pharmaceutical compositions and methods for producing antibodies to hepatitis b virus and kits and methods for detecting antibodies to hepatitis b virus
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
US5795745A (en) * 1979-07-05 1998-08-18 Genentech, Inc. Human growth hormone
US5424199A (en) * 1979-07-05 1995-06-13 Genentech, Inc. Human growth hormone
US6835557B1 (en) 1980-01-08 2004-12-28 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
US4469791A (en) * 1980-02-15 1984-09-04 Cpc International Inc. Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
US6455275B1 (en) 1980-02-25 2002-09-24 The Trustees Of Columbia University In The City Of New York DNA construct for producing proteinaceous materials in eucaryotic cells
US7588755B1 (en) 1980-04-03 2009-09-15 Biogen Idec Ma Inc. DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon-like polypeptides
US7635466B1 (en) * 1980-04-03 2009-12-22 Biogen Idec Ma Inc. DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon-like polypeptides
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
GB2133408A (en) * 1980-12-31 1984-07-25 Ilkka Palva Method for improving the production of proteins in bacillus
US5010015A (en) * 1980-12-31 1991-04-23 Genesit Oy Recombinant DNA-molecules and method for protein production
US5010000A (en) * 1980-12-31 1991-04-23 Genesit Oy Method for the preparation of a selected protein or a part thereof in Bacillus strain bacteria

Also Published As

Publication number Publication date
CH621147A5 (en) 1981-01-15
JPS51125782A (en) 1976-11-02
DK360175A (en) 1976-02-09
FR2281426B3 (en) 1979-06-15
DE2535554C2 (en) 1984-04-05
DE2535554A1 (en) 1976-02-26
SE7508905L (en) 1976-02-09
FR2281426A1 (en) 1976-03-05

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee