NZ187300A - Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism - Google Patents
Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organismInfo
- Publication number
- NZ187300A NZ187300A NZ187300A NZ18730078A NZ187300A NZ 187300 A NZ187300 A NZ 187300A NZ 187300 A NZ187300 A NZ 187300A NZ 18730078 A NZ18730078 A NZ 18730078A NZ 187300 A NZ187300 A NZ 187300A
- Authority
- NZ
- New Zealand
- Prior art keywords
- dna
- organism
- transfer vector
- gene
- nucleotide sequence
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title abstract 4
- 239000002773 nucleotide Substances 0.000 title abstract 3
- 125000003729 nucleotide group Chemical group 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 3
- 238000010804 cDNA synthesis Methods 0.000 abstract 3
- 239000002299 complementary DNA Substances 0.000 abstract 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80134377A | 1977-05-27 | 1977-05-27 | |
| US80502377A | 1977-06-09 | 1977-06-09 | |
| US89770978A | 1978-04-19 | 1978-04-19 | |
| US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
| US05/898,887 US4264731A (en) | 1977-05-27 | 1978-04-21 | DNA Joining method |
| US89900378A | 1978-04-26 | 1978-04-26 | |
| US89900278A | 1978-04-26 | 1978-04-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ187300A true NZ187300A (en) | 1982-08-17 |
Family
ID=27569921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ187300A NZ187300A (en) | 1977-05-27 | 1978-05-17 | Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPH0659218B2 (en) |
| AR (1) | AR225404A1 (en) |
| AU (1) | AU521903B2 (en) |
| BE (1) | BE867424A (en) |
| BG (1) | BG40319A3 (en) |
| CH (1) | CH642680A5 (en) |
| DD (1) | DD145927A5 (en) |
| DE (2) | DE2822568A1 (en) |
| DK (2) | DK233278A (en) |
| FI (1) | FI64640C (en) |
| FR (1) | FR2392033B1 (en) |
| GB (1) | GB1565190A (en) |
| GR (1) | GR73552B (en) |
| IL (1) | IL54790A (en) |
| IT (1) | IT1094934B (en) |
| LU (1) | LU79714A1 (en) |
| NL (1) | NL7805591A (en) |
| NZ (1) | NZ187300A (en) |
| PL (1) | PL140200B1 (en) |
| PT (1) | PT68082B (en) |
| SE (4) | SE451461B (en) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
| BR7807290A (en) * | 1977-11-08 | 1979-06-12 | Genentech Inc | PLASMIDEO, PROCESS FOR THE PRODUCTION OF A SPECIFIC POLYPEPTIDE CLONAL VEHICLE, TRANSFORMING BACTERIAL CULTURE, PROCESS TO PRODUCE AN IMMUNOGENIC SUBSTANCE, PROCESS TO PREPARE A STRUCTURAL, AND POLYDESOXIRRIBONUCLEOTIDE GENE |
| IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
| FR2458584A1 (en) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | VECTORS FOR THE TRANSFER AND EXPRESSION OF GENETIC MATERIAL IN EUKARYOTE CELL AND METHOD FOR THE PRODUCTION OF A PROTEIN DETERMINED IN EUKARYOTIC CELLS |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| GR70279B (en) * | 1979-09-12 | 1982-09-03 | Univ California | |
| IL58765A (en) * | 1979-11-21 | 1986-09-30 | Yeda Res & Dev | Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta |
| DE3001928A1 (en) * | 1980-01-19 | 1981-08-06 | Hoechst Ag, 6000 Frankfurt | GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| US4350764A (en) * | 1980-03-10 | 1982-09-21 | The Regents Of The University Of California | Microbiological synthesis of beta endorphin |
| CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| ATE22327T1 (en) * | 1980-12-12 | 1986-10-15 | Unilever Nv | DNA SEQUENCES FOR VARIOUS ALLEL FORMS OF MATURE THAUMATIN, RECOMBINANT PLASMIDS CONTAINING THESE SEQUENCES, METHODS FOR THE PRODUCTION OF THESE PLASMIDS, BACTERIAL CULTURES CONTAINING THESE RECOMBINANT PLASMIDS, AND METHODS FOR THE PRODUCTION OF MATURE THAUMATIN. |
| PH18602A (en) * | 1980-12-12 | 1985-08-21 | Unilever Nv | Structural genes encoding the various alleric naturation forms of preprothaumatin,recombinant cloning vehicles,comparing said structural genes and expression thereof in transformed microbial host cells |
| US4748233A (en) * | 1982-03-23 | 1988-05-31 | Bristol-Myers Company | Alpha-interferon Gx-1 |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| EP0103395A3 (en) * | 1982-08-17 | 1985-05-22 | Biogen N.V. | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| US5411951A (en) * | 1984-10-04 | 1995-05-02 | Monsanto Company | Prolonged release of biologically active somatotropin |
| US5474980A (en) * | 1984-10-04 | 1995-12-12 | Monsanto Company | Prolonged release of biologically active somatotropins |
| DK257988D0 (en) * | 1988-05-11 | 1988-05-11 | Novo Industri As | NEW PEPTIDES |
| DE4034702A1 (en) * | 1990-10-31 | 1992-05-07 | Siemens Ag | Testing active tools of bending machines, e.g. bending rams - holding tool in measurement position with magnetic field and using two sensors simultaneously in automated process |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
-
1978
- 1978-05-17 NZ NZ187300A patent/NZ187300A/en unknown
- 1978-05-23 NL NL7805591A patent/NL7805591A/en not_active Application Discontinuation
- 1978-05-23 AR AR272286A patent/AR225404A1/en active
- 1978-05-23 GR GR56309A patent/GR73552B/el unknown
- 1978-05-24 DE DE19782822568 patent/DE2822568A1/en active Granted
- 1978-05-24 BE BE187995A patent/BE867424A/en not_active IP Right Cessation
- 1978-05-24 DE DE2858357A patent/DE2858357C2/de not_active Expired - Lifetime
- 1978-05-24 PT PT68082A patent/PT68082B/en unknown
- 1978-05-25 DD DD78205588A patent/DD145927A5/en unknown
- 1978-05-25 AU AU36488/78A patent/AU521903B2/en not_active Expired
- 1978-05-25 GB GB22417/78A patent/GB1565190A/en not_active Expired
- 1978-05-25 FR FR7815596A patent/FR2392033B1/en not_active Expired
- 1978-05-26 FI FI781675A patent/FI64640C/en not_active IP Right Cessation
- 1978-05-26 SE SE7806086A patent/SE451461B/en unknown
- 1978-05-26 IL IL54790A patent/IL54790A/en unknown
- 1978-05-26 CH CH581078A patent/CH642680A5/en not_active IP Right Cessation
- 1978-05-26 DK DK782332A patent/DK233278A/en not_active Application Discontinuation
- 1978-05-26 LU LU79714A patent/LU79714A1/en unknown
- 1978-05-27 BG BG039878A patent/BG40319A3/en unknown
- 1978-05-27 JP JP53063756A patent/JPH0659218B2/en not_active Expired - Lifetime
- 1978-05-27 PL PL1978207176A patent/PL140200B1/en unknown
- 1978-05-29 IT IT23930/78A patent/IT1094934B/en active
-
1983
- 1983-09-29 SE SE8305327A patent/SE8305327L/en not_active Application Discontinuation
- 1983-09-29 SE SE8305329A patent/SE455794B/en not_active Application Discontinuation
- 1983-09-29 SE SE8305328A patent/SE8305328D0/en not_active Application Discontinuation
-
1991
- 1991-01-04 DK DK000791A patent/DK791A/en not_active Application Discontinuation
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