BG40319A3 - Method for preparing recombinant desoxynucleinic acid containing genes of high organism and its cloning in microorganisms - Google Patents

Method for preparing recombinant desoxynucleinic acid containing genes of high organism and its cloning in microorganisms

Info

Publication number
BG40319A3
BG40319A3 BG039878A BG3987878A BG40319A3 BG 40319 A3 BG40319 A3 BG 40319A3 BG 039878 A BG039878 A BG 039878A BG 3987878 A BG3987878 A BG 3987878A BG 40319 A3 BG40319 A3 BG 40319A3
Authority
BG
Bulgaria
Prior art keywords
cloning
containing genes
desoxynucleinic
microorganisms
acid containing
Prior art date
Application number
BG039878A
Other languages
Bulgarian (bg)
Inventor
William Rutter
Howard Goodman
Axel Ullrich
John Shine
John Chirgwin
Raymond Pictet
Peter Seeburg
Original Assignee
Irons And Sears P.C
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05897710 external-priority patent/US4363877B1/en
Priority claimed from US05/898,887 external-priority patent/US4264731A/en
Application filed by Irons And Sears P.C filed Critical Irons And Sears P.C
Publication of BG40319A3 publication Critical patent/BG40319A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57518—Placental lactogen; Chorionic somatomammotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Diabetes (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Метод за получаване на рекомбинантна днк,съдържаща гени от висш организъм, и клонирането й в микроорганизъм, съгласно който се изолират клетки от специализирани тъкани на организма, екстрахира се и рнк от клетките, кодираща специфичен продукт, синтезира се двуверижна днк, извършва се пренос на вектор на днк, характеризиращ се с това, че екстрахирането на ирнк от клетките се извършва в присъствие на рн-азна инхибиторна смес и получената сднк се извършва с преносен вектор на днк, подходящ за внасяне на микроорганизъм. Патентните претенции са формулирани в 15 точки.A method for obtaining recombinant DNA containing genes from a higher organism and its cloning in a microorganism, according to which cells are isolated from specialized tissues of the organism, RNA encoding a specific product is extracted from the cells, double-stranded DNA is synthesized, transfer of a DNA vector, characterized in that the extraction of irnk from the cells is carried out in the presence of a pH-ase inhibitor mixture and the resulting cDNA is carried out with a DNA transfer vector suitable for introducing a microorganism. The patent claims are formulated in 15 points.

BG039878A 1977-05-27 1978-05-27 Method for preparing recombinant desoxynucleinic acid containing genes of high organism and its cloning in microorganisms BG40319A3 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US80134377A 1977-05-27 1977-05-27
US80502377A 1977-06-09 1977-06-09
US89770978A 1978-04-19 1978-04-19
US05897710 US4363877B1 (en) 1977-09-23 1978-04-19 Recombinant dna transfer vectors
US05/898,887 US4264731A (en) 1977-05-27 1978-04-21 DNA Joining method
US89900378A 1978-04-26 1978-04-26
US89900278A 1978-04-26 1978-04-26

Publications (1)

Publication Number Publication Date
BG40319A3 true BG40319A3 (en) 1986-11-14

Family

ID=27569921

Family Applications (1)

Application Number Title Priority Date Filing Date
BG039878A BG40319A3 (en) 1977-05-27 1978-05-27 Method for preparing recombinant desoxynucleinic acid containing genes of high organism and its cloning in microorganisms

Country Status (21)

Country Link
JP (1) JPH0659218B2 (en)
AR (1) AR225404A1 (en)
AU (1) AU521903B2 (en)
BE (1) BE867424A (en)
BG (1) BG40319A3 (en)
CH (1) CH642680A5 (en)
DD (1) DD145927A5 (en)
DE (2) DE2822568A1 (en)
DK (2) DK233278A (en)
FI (1) FI64640C (en)
FR (1) FR2392033B1 (en)
GB (1) GB1565190A (en)
GR (1) GR73552B (en)
IL (1) IL54790A (en)
IT (1) IT1094934B (en)
LU (1) LU79714A1 (en)
NL (1) NL7805591A (en)
NZ (1) NZ187300A (en)
PL (1) PL140200B1 (en)
PT (1) PT68082B (en)
SE (4) SE451461B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
BR7807290A (en) * 1977-11-08 1979-06-12 Genentech Inc PLASMIDEO, PROCESS FOR THE PRODUCTION OF A SPECIFIC POLYPEPTIDE CLONAL VEHICLE, TRANSFORMING BACTERIAL CULTURE, PROCESS TO PRODUCE AN IMMUNOGENIC SUBSTANCE, PROCESS TO PREPARE A STRUCTURAL, AND POLYDESOXIRRIBONUCLEOTIDE GENE
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
US6297355B1 (en) 1978-12-22 2001-10-02 Biogen, Inc. Polypeptides displaying HBV antigenicity or hbv antigen specificity
JPS55150883A (en) * 1979-05-11 1980-11-25 Nakano Vinegar Co Ltd Method of storing "mozuku"
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
FR2458584A1 (en) * 1979-06-08 1981-01-02 Pasteur Institut VECTORS FOR THE TRANSFER AND EXPRESSION OF GENETIC MATERIAL IN EUKARYOTE CELL AND METHOD FOR THE PRODUCTION OF A PROTEIN DETERMINED IN EUKARYOTIC CELLS
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
GR70279B (en) * 1979-09-12 1982-09-03 Univ California
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
DE3001928A1 (en) * 1980-01-19 1981-08-06 Hoechst Ag, 6000 Frankfurt GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
CA1202581A (en) * 1980-03-27 1986-04-01 Saran A. Narang Adaptor molecules for dna and their application to synthesis of gene-derived products
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
PH18602A (en) * 1980-12-12 1985-08-21 Unilever Nv Structural genes encoding the various alleric naturation forms of preprothaumatin,recombinant cloning vehicles,comparing said structural genes and expression thereof in transformed microbial host cells
EP0054330B1 (en) * 1980-12-12 1986-09-17 Unilever N.V. Dna sequences encoding various allelic forms of mature thaumatin, recombinant plasmids comprising said dna's and a process for their preparation, bacterial cultures comprising said recombinant plasmids, and method for producing mature thaumatin
US4695543A (en) * 1982-03-23 1987-09-22 Bristol-Myers Company Alpha Interferon GX-1
US4748233A (en) * 1982-03-23 1988-05-31 Bristol-Myers Company Alpha-interferon Gx-1
CA1224432A (en) * 1982-08-17 1987-07-21 Gary N. Buell Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield
US5411951A (en) * 1984-10-04 1995-05-02 Monsanto Company Prolonged release of biologically active somatotropin
US5474980A (en) * 1984-10-04 1995-12-12 Monsanto Company Prolonged release of biologically active somatotropins
DK257988D0 (en) * 1988-05-11 1988-05-11 Novo Industri As NEW PEPTIDES
DE4034702A1 (en) * 1990-10-31 1992-05-07 Siemens Ag Testing active tools of bending machines, e.g. bending rams - holding tool in measurement position with magnetic field and using two sensors simultaneously in automated process

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment

Also Published As

Publication number Publication date
AU3648878A (en) 1979-11-29
AU521903B2 (en) 1982-05-06
SE455794B (en) 1988-08-08
DK791D0 (en) 1991-01-04
PL207176A1 (en) 1979-03-26
FI64640C (en) 1983-12-12
SE8305328L (en) 1983-09-29
SE8305329L (en) 1983-09-29
DK791A (en) 1991-01-04
NZ187300A (en) 1982-08-17
SE8305327L (en) 1983-09-29
PL140200B1 (en) 1987-04-30
PT68082B (en) 1980-02-20
DD145927A5 (en) 1981-01-14
NL7805591A (en) 1978-11-29
FI781675A (en) 1978-11-28
DE2822568A1 (en) 1978-12-14
FI64640B (en) 1983-08-31
FR2392033A1 (en) 1978-12-22
FR2392033B1 (en) 1985-09-13
SE7806086L (en) 1978-11-28
DK233278A (en) 1978-11-28
BE867424A (en) 1978-09-18
CH642680A5 (en) 1984-04-30
SE8305328D0 (en) 1983-09-29
GR73552B (en) 1984-03-14
SE8305327D0 (en) 1983-09-29
JPH0659218B2 (en) 1994-08-10
DE2822568C2 (en) 1989-08-31
GB1565190A (en) 1980-04-16
JPS5449387A (en) 1979-04-18
AR225404A1 (en) 1982-03-31
IT1094934B (en) 1985-08-10
LU79714A1 (en) 1978-11-06
IL54790A (en) 1983-07-31
PT68082A (en) 1978-06-01
SE8305329D0 (en) 1983-09-29
IL54790A0 (en) 1978-07-31
DE2858357C2 (en) 1990-03-15
IT7823930A0 (en) 1978-05-29
SE451461B (en) 1987-10-12

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