JPS5449387A - Dna introducing vector - Google Patents
Dna introducing vectorInfo
- Publication number
- JPS5449387A JPS5449387A JP6375678A JP6375678A JPS5449387A JP S5449387 A JPS5449387 A JP S5449387A JP 6375678 A JP6375678 A JP 6375678A JP 6375678 A JP6375678 A JP 6375678A JP S5449387 A JPS5449387 A JP S5449387A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- sub
- sequence
- microorganisms
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004635 Complementary DNA Proteins 0.000 abstract 3
- 238000010804 cDNA synthesis Methods 0.000 abstract 3
- 239000002299 complementary DNA Substances 0.000 abstract 3
- 244000005700 microbiome Species 0.000 abstract 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.
Applications Claiming Priority (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80134377A | 1977-05-27 | 1977-05-27 | |
| US80502377A | 1977-06-09 | 1977-06-09 | |
| US89770978A | 1978-04-19 | 1978-04-19 | |
| US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
| US05/898,887 US4264731A (en) | 1977-05-27 | 1978-04-21 | DNA Joining method |
| US89900378A | 1978-04-26 | 1978-04-26 | |
| US89900278A | 1978-04-26 | 1978-04-26 | |
| US899002 | 1978-04-26 | ||
| US805023 | 1978-04-26 | ||
| US897709 | 1978-04-26 | ||
| US898887 | 1978-04-26 | ||
| US801343 | 1985-11-25 | ||
| US897710 | 1986-08-18 | ||
| US899003 | 1986-08-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5449387A true JPS5449387A (en) | 1979-04-18 |
| JPH0659218B2 JPH0659218B2 (en) | 1994-08-10 |
Family
ID=27569921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP53063756A Expired - Lifetime JPH0659218B2 (en) | 1977-05-27 | 1978-05-27 | DNA transfer vector |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPH0659218B2 (en) |
| AR (1) | AR225404A1 (en) |
| AU (1) | AU521903B2 (en) |
| BE (1) | BE867424A (en) |
| BG (1) | BG40319A3 (en) |
| CH (1) | CH642680A5 (en) |
| DD (1) | DD145927A5 (en) |
| DE (2) | DE2822568A1 (en) |
| DK (2) | DK233278A (en) |
| FI (1) | FI64640C (en) |
| FR (1) | FR2392033B1 (en) |
| GB (1) | GB1565190A (en) |
| GR (1) | GR73552B (en) |
| IL (1) | IL54790A (en) |
| IT (1) | IT1094934B (en) |
| LU (1) | LU79714A1 (en) |
| NL (1) | NL7805591A (en) |
| NZ (1) | NZ187300A (en) |
| PL (1) | PL140200B1 (en) |
| PT (1) | PT68082B (en) |
| SE (4) | SE451461B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| JPS5636499A (en) * | 1979-06-01 | 1981-04-09 | Univ California | Dna introduced vector |
| JPS5695199A (en) * | 1979-09-12 | 1981-08-01 | Univ California | Human gene having proinsulinn and preproinsulinnproducing code |
| JPS56164200A (en) * | 1980-03-10 | 1981-12-17 | Univ California | Biological synthesis of beta-endolphin |
| JPH02478A (en) * | 1979-07-05 | 1990-01-05 | Genentech Inc | Production of peptide by manifestation of partial synthetic gene |
| JPH02211879A (en) * | 1979-11-21 | 1990-08-23 | Yeda Res & Dev Co Ltd | Cdna of human interferon beta 2 and preparation thereof |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
| BR7807290A (en) * | 1977-11-08 | 1979-06-12 | Genentech Inc | PLASMIDEO, PROCESS FOR THE PRODUCTION OF A SPECIFIC POLYPEPTIDE CLONAL VEHICLE, TRANSFORMING BACTERIAL CULTURE, PROCESS TO PRODUCE AN IMMUNOGENIC SUBSTANCE, PROCESS TO PREPARE A STRUCTURAL, AND POLYDESOXIRRIBONUCLEOTIDE GENE |
| IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| FR2458584A1 (en) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | VECTORS FOR THE TRANSFER AND EXPRESSION OF GENETIC MATERIAL IN EUKARYOTE CELL AND METHOD FOR THE PRODUCTION OF A PROTEIN DETERMINED IN EUKARYOTIC CELLS |
| DE3001928A1 (en) * | 1980-01-19 | 1981-08-06 | Hoechst Ag, 6000 Frankfurt | GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| PH18602A (en) * | 1980-12-12 | 1985-08-21 | Unilever Nv | Structural genes encoding the various alleric naturation forms of preprothaumatin,recombinant cloning vehicles,comparing said structural genes and expression thereof in transformed microbial host cells |
| EP0054330B1 (en) * | 1980-12-12 | 1986-09-17 | Unilever N.V. | Dna sequences encoding various allelic forms of mature thaumatin, recombinant plasmids comprising said dna's and a process for their preparation, bacterial cultures comprising said recombinant plasmids, and method for producing mature thaumatin |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| US4748233A (en) * | 1982-03-23 | 1988-05-31 | Bristol-Myers Company | Alpha-interferon Gx-1 |
| CA1224432A (en) * | 1982-08-17 | 1987-07-21 | Gary N. Buell | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| US5411951A (en) * | 1984-10-04 | 1995-05-02 | Monsanto Company | Prolonged release of biologically active somatotropin |
| US5474980A (en) * | 1984-10-04 | 1995-12-12 | Monsanto Company | Prolonged release of biologically active somatotropins |
| DK257988D0 (en) * | 1988-05-11 | 1988-05-11 | Novo Industri As | NEW PEPTIDES |
| DE4034702A1 (en) * | 1990-10-31 | 1992-05-07 | Siemens Ag | Testing active tools of bending machines, e.g. bending rams - holding tool in measurement position with magnetic field and using two sensors simultaneously in automated process |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51125782A (en) * | 1974-08-08 | 1976-11-02 | Ici Ltd | Hereditary modification of microorganism |
-
1978
- 1978-05-17 NZ NZ187300A patent/NZ187300A/en unknown
- 1978-05-23 GR GR56309A patent/GR73552B/el unknown
- 1978-05-23 AR AR272286A patent/AR225404A1/en active
- 1978-05-23 NL NL7805591A patent/NL7805591A/en not_active Application Discontinuation
- 1978-05-24 BE BE187995A patent/BE867424A/en not_active IP Right Cessation
- 1978-05-24 DE DE19782822568 patent/DE2822568A1/en active Granted
- 1978-05-24 PT PT68082A patent/PT68082B/en unknown
- 1978-05-24 DE DE2858357A patent/DE2858357C2/de not_active Expired - Lifetime
- 1978-05-25 GB GB22417/78A patent/GB1565190A/en not_active Expired
- 1978-05-25 DD DD78205588A patent/DD145927A5/en unknown
- 1978-05-25 AU AU36488/78A patent/AU521903B2/en not_active Expired
- 1978-05-25 FR FR7815596A patent/FR2392033B1/en not_active Expired
- 1978-05-26 SE SE7806086A patent/SE451461B/en unknown
- 1978-05-26 LU LU79714A patent/LU79714A1/en unknown
- 1978-05-26 DK DK782332A patent/DK233278A/en not_active Application Discontinuation
- 1978-05-26 CH CH581078A patent/CH642680A5/en not_active IP Right Cessation
- 1978-05-26 IL IL54790A patent/IL54790A/en unknown
- 1978-05-26 FI FI781675A patent/FI64640C/en not_active IP Right Cessation
- 1978-05-27 BG BG039878A patent/BG40319A3/en unknown
- 1978-05-27 PL PL1978207176A patent/PL140200B1/en unknown
- 1978-05-27 JP JP53063756A patent/JPH0659218B2/en not_active Expired - Lifetime
- 1978-05-29 IT IT23930/78A patent/IT1094934B/en active
-
1983
- 1983-09-29 SE SE8305329A patent/SE455794B/en not_active Application Discontinuation
- 1983-09-29 SE SE8305327A patent/SE8305327D0/en not_active Application Discontinuation
- 1983-09-29 SE SE8305328A patent/SE8305328D0/en not_active Application Discontinuation
-
1991
- 1991-01-04 DK DK000791A patent/DK791A/en not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51125782A (en) * | 1974-08-08 | 1976-11-02 | Ici Ltd | Hereditary modification of microorganism |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| JPS5636499A (en) * | 1979-06-01 | 1981-04-09 | Univ California | Dna introduced vector |
| JPH02478A (en) * | 1979-07-05 | 1990-01-05 | Genentech Inc | Production of peptide by manifestation of partial synthetic gene |
| JPH05268970A (en) * | 1979-07-05 | 1993-10-19 | Genentech Inc | Replicable bacterial plasmid expressing human growth hormone gene |
| JPH0648987B2 (en) * | 1979-07-05 | 1994-06-29 | ジェネンテク・インコーポレイテッド | Method for producing peptide by expression of partially synthetic gene |
| JPS5695199A (en) * | 1979-09-12 | 1981-08-01 | Univ California | Human gene having proinsulinn and preproinsulinnproducing code |
| JPH02211879A (en) * | 1979-11-21 | 1990-08-23 | Yeda Res & Dev Co Ltd | Cdna of human interferon beta 2 and preparation thereof |
| JPS56164200A (en) * | 1980-03-10 | 1981-12-17 | Univ California | Biological synthesis of beta-endolphin |
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