SE8305327D0 - INSULATION OF DIFFERENTIATED CELLS - Google Patents
INSULATION OF DIFFERENTIATED CELLSInfo
- Publication number
- SE8305327D0 SE8305327D0 SE8305327A SE8305327A SE8305327D0 SE 8305327 D0 SE8305327 D0 SE 8305327D0 SE 8305327 A SE8305327 A SE 8305327A SE 8305327 A SE8305327 A SE 8305327A SE 8305327 D0 SE8305327 D0 SE 8305327D0
- Authority
- SE
- Sweden
- Prior art keywords
- dna
- sub
- sequence
- microorganisms
- nucleotide
- Prior art date
Links
- 238000009413 insulation Methods 0.000 title 1
- 108020004635 Complementary DNA Proteins 0.000 abstract 3
- 238000010804 cDNA synthesis Methods 0.000 abstract 3
- 239000002299 complementary DNA Substances 0.000 abstract 3
- 244000005700 microbiome Species 0.000 abstract 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80134377A | 1977-05-27 | 1977-05-27 | |
| US80502377A | 1977-06-09 | 1977-06-09 | |
| US89770978A | 1978-04-19 | 1978-04-19 | |
| US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
| US05/898,887 US4264731A (en) | 1977-05-27 | 1978-04-21 | DNA Joining method |
| US89900378A | 1978-04-26 | 1978-04-26 | |
| US89900278A | 1978-04-26 | 1978-04-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8305327L SE8305327L (en) | 1983-09-29 |
| SE8305327D0 true SE8305327D0 (en) | 1983-09-29 |
Family
ID=27569921
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806086A SE451461B (en) | 1977-05-27 | 1978-05-26 | PLASMID WITH THE NUCLEOTIDE SEQUENCE INCLUDES A SEQUENCE CODING FOR A- OR B-CHAIN IN INSULIN AND PROCEDURE FOR ITS PREPARATION AND E.COLI CONTAINING THE PLASMID AND PROCEDURE FOR PRODUCING E.COLI |
| SE8305327A SE8305327D0 (en) | 1977-05-27 | 1983-09-29 | INSULATION OF DIFFERENTIATED CELLS |
| SE8305329A SE455794B (en) | 1977-05-27 | 1983-09-29 | PLASMID WITH NUCLEOTIDE SEQUENCE CODING THE AMINO ACID SEQUENCE IN HUMAN GROWTH HORMON AND PROCEDURE FOR PRODUCING THEREOF |
| SE8305328A SE8305328D0 (en) | 1977-05-27 | 1983-09-29 | ISOLATION OF NON-DRAINED RNA |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806086A SE451461B (en) | 1977-05-27 | 1978-05-26 | PLASMID WITH THE NUCLEOTIDE SEQUENCE INCLUDES A SEQUENCE CODING FOR A- OR B-CHAIN IN INSULIN AND PROCEDURE FOR ITS PREPARATION AND E.COLI CONTAINING THE PLASMID AND PROCEDURE FOR PRODUCING E.COLI |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8305329A SE455794B (en) | 1977-05-27 | 1983-09-29 | PLASMID WITH NUCLEOTIDE SEQUENCE CODING THE AMINO ACID SEQUENCE IN HUMAN GROWTH HORMON AND PROCEDURE FOR PRODUCING THEREOF |
| SE8305328A SE8305328D0 (en) | 1977-05-27 | 1983-09-29 | ISOLATION OF NON-DRAINED RNA |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPH0659218B2 (en) |
| AR (1) | AR225404A1 (en) |
| AU (1) | AU521903B2 (en) |
| BE (1) | BE867424A (en) |
| BG (1) | BG40319A3 (en) |
| CH (1) | CH642680A5 (en) |
| DD (1) | DD145927A5 (en) |
| DE (2) | DE2822568A1 (en) |
| DK (2) | DK233278A (en) |
| FI (1) | FI64640C (en) |
| FR (1) | FR2392033B1 (en) |
| GB (1) | GB1565190A (en) |
| GR (1) | GR73552B (en) |
| IL (1) | IL54790A (en) |
| IT (1) | IT1094934B (en) |
| LU (1) | LU79714A1 (en) |
| NL (1) | NL7805591A (en) |
| NZ (1) | NZ187300A (en) |
| PL (1) | PL140200B1 (en) |
| PT (1) | PT68082B (en) |
| SE (4) | SE451461B (en) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
| RO80052B (en) * | 1977-11-08 | 1985-03-30 | Genetech | Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator |
| IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| ZA802992B (en) * | 1979-06-01 | 1981-10-28 | Univ California | Human pre-growth hormone |
| FR2458584A1 (en) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | VECTORS FOR THE TRANSFER AND EXPRESSION OF GENETIC MATERIAL IN EUKARYOTE CELL AND METHOD FOR THE PRODUCTION OF A PROTEIN DETERMINED IN EUKARYOTIC CELLS |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| GR70279B (en) * | 1979-09-12 | 1982-09-03 | Univ California | |
| IL58765A (en) * | 1979-11-21 | 1986-09-30 | Yeda Res & Dev | Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta |
| DE3001928A1 (en) * | 1980-01-19 | 1981-08-06 | Hoechst Ag, 6000 Frankfurt | GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| US4350764A (en) * | 1980-03-10 | 1982-09-21 | The Regents Of The University Of California | Microbiological synthesis of beta endorphin |
| CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| EP0054331B1 (en) * | 1980-12-12 | 1992-06-03 | Unilever N.V. | Structural genes encoding the various allelic and maturation forms of preprothaumatin and mutations thereof, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells |
| IE52417B1 (en) * | 1980-12-12 | 1987-10-28 | Unilever Plc | Dna sequences encoding various allelic forms of mature thaumatin,recombinant plasmids comprising said dnas and a process for their preparation,bacterial cultures comprising said recombinant plasmids,and method for producing mature thaumatin |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| US4748233A (en) * | 1982-03-23 | 1988-05-31 | Bristol-Myers Company | Alpha-interferon Gx-1 |
| EP0103395A3 (en) * | 1982-08-17 | 1985-05-22 | Biogen N.V. | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| US5411951A (en) * | 1984-10-04 | 1995-05-02 | Monsanto Company | Prolonged release of biologically active somatotropin |
| US5474980A (en) * | 1984-10-04 | 1995-12-12 | Monsanto Company | Prolonged release of biologically active somatotropins |
| DK257988D0 (en) * | 1988-05-11 | 1988-05-11 | Novo Industri As | NEW PEPTIDES |
| DE4034702A1 (en) * | 1990-10-31 | 1992-05-07 | Siemens Ag | Testing active tools of bending machines, e.g. bending rams - holding tool in measurement position with magnetic field and using two sensors simultaneously in automated process |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
-
1978
- 1978-05-17 NZ NZ187300A patent/NZ187300A/en unknown
- 1978-05-23 AR AR272286A patent/AR225404A1/en active
- 1978-05-23 NL NL7805591A patent/NL7805591A/en not_active Application Discontinuation
- 1978-05-23 GR GR56309A patent/GR73552B/el unknown
- 1978-05-24 DE DE19782822568 patent/DE2822568A1/en active Granted
- 1978-05-24 PT PT68082A patent/PT68082B/en unknown
- 1978-05-24 BE BE187995A patent/BE867424A/en not_active IP Right Cessation
- 1978-05-24 DE DE2858357A patent/DE2858357C2/de not_active Expired - Lifetime
- 1978-05-25 FR FR7815596A patent/FR2392033B1/en not_active Expired
- 1978-05-25 AU AU36488/78A patent/AU521903B2/en not_active Expired
- 1978-05-25 GB GB22417/78A patent/GB1565190A/en not_active Expired
- 1978-05-25 DD DD78205588A patent/DD145927A5/en unknown
- 1978-05-26 FI FI781675A patent/FI64640C/en not_active IP Right Cessation
- 1978-05-26 DK DK782332A patent/DK233278A/en not_active Application Discontinuation
- 1978-05-26 LU LU79714A patent/LU79714A1/en unknown
- 1978-05-26 SE SE7806086A patent/SE451461B/en unknown
- 1978-05-26 CH CH581078A patent/CH642680A5/en not_active IP Right Cessation
- 1978-05-26 IL IL54790A patent/IL54790A/en unknown
- 1978-05-27 JP JP53063756A patent/JPH0659218B2/en not_active Expired - Lifetime
- 1978-05-27 PL PL1978207176A patent/PL140200B1/en unknown
- 1978-05-27 BG BG7839878A patent/BG40319A3/en unknown
- 1978-05-29 IT IT23930/78A patent/IT1094934B/en active
-
1983
- 1983-09-29 SE SE8305327A patent/SE8305327D0/en not_active Application Discontinuation
- 1983-09-29 SE SE8305329A patent/SE455794B/en not_active Application Discontinuation
- 1983-09-29 SE SE8305328A patent/SE8305328D0/en not_active Application Discontinuation
-
1991
- 1991-01-04 DK DK000791A patent/DK791A/en not_active Application Discontinuation
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SE8305327D0 (en) | INSULATION OF DIFFERENTIATED CELLS | |
| Yamanaka et al. | Crystalline Pseudomonas cytochrome oxidase: I. Enzymic properties with special reference to the biological specificity | |
| WO2003008614A3 (en) | Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced suca or sucb gene | |
| KR830005351A (en) | Method for producing bacterial polypeptide using tryptophan promoting gene-operator gene | |
| Kletzin | Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic Archaeum Desulfurolobus ambivalens | |
| YU55100A (en) | PROCEDURE FOR THE FERMENTATION PRODUCTION OF d-PANTOTHENIC ACID BY USING BACTERIA IN CORIN FORM | |
| Beaudry et al. | An efficient strategy for the synthesis of circular RNA molecules. | |
| WO2003008604A3 (en) | Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an attenuated aceb gene | |
| ATE92530T1 (en) | TRANSFORMATION AND EXPRESSION OF A FOREIGN GENE IN -I(BRASSICA) SPECIES. | |
| HUP9800329A2 (en) | Nucleic acid constructs for the cell cycle regulated expression of structural genes | |
| CN108611434B (en) | Sisal hemp internal reference gene ACT/GAPDH and application thereof | |
| CN105441371B (en) | A kind of genetic engineering bacterium and its application in production Co-Q10 | |
| CN108277244B (en) | Preparation of sedoheptulose and aldose by immobilized enzyme cascade reaction | |
| Dube et al. | A structural requirement for codon-anticodon interaction on the ribosome | |
| CN101550413A (en) | Method for extraction and purification of microbial total DNA of compost and buffer solution thereof | |
| DE3751326D1 (en) | Amylolytic enzyme-producing microorganisms constructed by recombinant DNA technology and their use in fermentation processes. | |
| JPS5452789A (en) | Preparation of sarcosine oxidase | |
| Kwon et al. | Relationship between changes in microbial community and the deterioration of methane fermentation which treats synthetic peptone wastewater | |
| CN114958791B (en) | Spermidine derivative glycosyltransferase LbUGT62 and encoding gene and application thereof | |
| ES449890A1 (en) | Treatment of single cell protein | |
| CN112980754A (en) | Method for preparing inositol by catalyzing starch with bacillus subtilis whole cells | |
| CN105695451B (en) | The extracting method of microorganism total DNA in a kind of human stomach | |
| CN108033999A (en) | A kind of cause a disease with Rhizoctonia solani Kuhn relevant effector R1YBU0 and its encoding gene | |
| CN104726466A (en) | Antrodia Biotin protein gene and cloning and sequencing method thereof | |
| CN107955065A (en) | A kind of cause a disease with Rhizoctonia solani Kuhn relevant effector R1YBX6 and its encoding gene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed |
Ref document number: 8305327-2 Effective date: 19850910 |