RO80052B - Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator - Google Patents

Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator

Info

Publication number
RO80052B
RO80052B RO95604A RO9560478A RO80052B RO 80052 B RO80052 B RO 80052B RO 95604 A RO95604 A RO 95604A RO 9560478 A RO9560478 A RO 9560478A RO 80052 B RO80052 B RO 80052B
Authority
RO
Romania
Prior art keywords
plasmid
transforming
generator
heterologous polypeptide
bacterial host
Prior art date
Application number
RO95604A
Other languages
Romanian (ro)
Other versions
RO80052A (en
Inventor
Keiichi Itakura
Arthur Dale Riggs
Original Assignee
Genetech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetech filed Critical Genetech
Publication of RO80052A publication Critical patent/RO80052A/en
Publication of RO80052B publication Critical patent/RO80052B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/655—Somatostatins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Inventia se refera la un procedeu pentru prepararea unui vehicul de clonare pentru transformarea unei gazde bacteriene pentru a deveni generator de polipeptida heterologa, procedeu ce include tratarea unei plasmide cu o enzima restrictiva, obtinerea zisei plasmide în prezenta de ADN dublu caternar aditional, în modul acesta fiind înmcorporata secventa aditionala în plasmida realizata, aceasta plasmida fiind capabila, dupa transfoemare în organismul gazda, sa fie amplificata prin multiplicare. Asezarea în plasmida a unui regulator omolog fata de bacteria gazda, în starea sa netransformata, plasînd în faza de citire cu regulatorul o inserttie de ADN, care codifica secventa de aminoacizi ai polipeptidei heterogene.The invention relates to a process for the preparation of a cloning vehicle for transforming a bacterial host to become a heterologous polypeptide generator, which process comprises treating a plasmid with a restriction enzyme, obtaining the plasmid in the presence of additional double-stranded DNA in this fashion adding the additional sequence to the resulting plasmid, this plasmid being able, after transfusion in the host organism, to be amplified by multiplication. Placing plasmid of a homologous regulator relative to the host bacterium in its unmodified state by placing in the read phase with the regulator a DNA insert which encodes the amino acid sequence of the heterogeneous polypeptide.

RO95604A 1977-11-08 1978-11-06 Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator RO80052B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84969177A 1977-11-08 1977-11-08
US84969277A 1977-11-08 1977-11-08
US84959177A 1977-11-08 1977-11-08

Publications (2)

Publication Number Publication Date
RO80052A RO80052A (en) 1985-03-15
RO80052B true RO80052B (en) 1985-03-30

Family

ID=27420338

Family Applications (1)

Application Number Title Priority Date Filing Date
RO95604A RO80052B (en) 1977-11-08 1978-11-06 Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator

Country Status (7)

Country Link
AU (2) AU527165B2 (en)
BR (1) BR7807290A (en)
FR (3) FR2422717B1 (en)
LU (1) LU80489A1 (en)
OA (1) OA06002A (en)
RO (1) RO80052B (en)
ZM (1) ZM9578A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK159976C (en) * 1979-06-01 1991-05-27 Searle & Co PLASMID VECTORS, METHOD OF PRODUCING THEREOF, BACTERY CELLS TRANSFORMED WITH PLASMID VECTORS, AND EXPRESSION OF PROTEIN IN TRANSFORMED BACTERY CELLS
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4645828A (en) * 1984-03-23 1987-02-24 Oncogen Platelet related growth regulator
AU580064B2 (en) * 1984-06-04 1988-12-22 Lubrizol Genetics Inc. Nitrogen fixation regulator genes
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
US4987070A (en) * 1987-03-04 1991-01-22 Suntory Limited Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
BR7807288A (en) * 1977-11-08 1979-06-12 Genentech Inc POLYNUCLEOTIDE SYNTHESIS PROCESS
FR2422685A1 (en) * 1978-04-14 1979-11-09 Pasteur Institut VECTORS, DERIVED FROM LAMBDA BACTERIOPHAGUS, ALLOWING THE INSERTION AND EXPRESSION OF A GENE, PROCARYOTE OR EUKARYOTE, UNDER THE CONTROL OF THE PROMOTER AND THE OPERATOR OF THE LACTOSE OPERON OF ESCHERICHIA COLI BACTERIA
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
FR2428075A1 (en) * 1978-06-08 1980-01-04 Pasteur Institut PROCESS FOR THE PRODUCTION OF PROTEINS BY EXPRESSION OF THE CORRESPONDING GENES IN BACTERIA AND VECTORS LIKELY TO BE USED IN SUCH METHODS
GR71912B (en) * 1978-08-11 1983-08-16 Univ California
GB2033905B (en) * 1978-08-21 1982-10-13 Upjohn Co Bacterial preparation of proteins

Also Published As

Publication number Publication date
AU7448481A (en) 1982-01-14
AU4136178A (en) 1979-05-17
FR2422719A1 (en) 1979-11-09
FR2422717B1 (en) 1985-12-06
AU546092B2 (en) 1985-08-15
ZM9578A1 (en) 1980-03-21
LU80489A1 (en) 1979-03-22
RO80052A (en) 1985-03-15
AU527165B2 (en) 1983-02-17
FR2422719B1 (en) 1984-12-28
FR2422718B1 (en) 1984-05-18
FR2422717A1 (en) 1979-11-09
OA06002A (en) 1981-06-30
FR2422718A1 (en) 1979-11-09
BR7807290A (en) 1979-06-12

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