RO80052B - Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator - Google Patents

Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator

Info

Publication number
RO80052B
RO80052B RO95604A RO9560478A RO80052B RO 80052 B RO80052 B RO 80052B RO 95604 A RO95604 A RO 95604A RO 9560478 A RO9560478 A RO 9560478A RO 80052 B RO80052 B RO 80052B
Authority
RO
Romania
Prior art keywords
plasmid
transforming
generator
heterologous polypeptide
bacterial host
Prior art date
Application number
RO95604A
Other languages
Romanian (ro)
Other versions
RO80052A (en
Inventor
Keiichi Itakura
Arthur Dale Riggs
Original Assignee
Genetech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetech filed Critical Genetech
Publication of RO80052A publication Critical patent/RO80052A/en
Publication of RO80052B publication Critical patent/RO80052B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/655—Somatostatins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Inventia se refera la un procedeu pentru prepararea unui vehicul de clonare pentru transformarea unei gazde bacteriene pentru a deveni generator de polipeptida heterologa, procedeu ce include tratarea unei plasmide cu o enzima restrictiva, obtinerea zisei plasmide în prezenta de ADN dublu caternar aditional, în modul acesta fiind înmcorporata secventa aditionala în plasmida realizata, aceasta plasmida fiind capabila, dupa transfoemare în organismul gazda, sa fie amplificata prin multiplicare. Asezarea în plasmida a unui regulator omolog fata de bacteria gazda, în starea sa netransformata, plasînd în faza de citire cu regulatorul o inserttie de ADN, care codifica secventa de aminoacizi ai polipeptidei heterogene.The invention relates to a process for the preparation of a cloning vehicle for transforming a bacterial host to become a heterologous polypeptide generator, which process comprises treating a plasmid with a restriction enzyme, obtaining the plasmid in the presence of additional double-stranded DNA in this fashion adding the additional sequence to the resulting plasmid, this plasmid being able, after transfusion in the host organism, to be amplified by multiplication. Placing plasmid of a homologous regulator relative to the host bacterium in its unmodified state by placing in the read phase with the regulator a DNA insert which encodes the amino acid sequence of the heterogeneous polypeptide.

RO95604A 1977-11-08 1978-11-06 Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator RO80052B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84969277A 1977-11-08 1977-11-08
US84959177A 1977-11-08 1977-11-08
US84969177A 1977-11-08 1977-11-08

Publications (2)

Publication Number Publication Date
RO80052A RO80052A (en) 1985-03-15
RO80052B true RO80052B (en) 1985-03-30

Family

ID=27420338

Family Applications (1)

Application Number Title Priority Date Filing Date
RO95604A RO80052B (en) 1977-11-08 1978-11-06 Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator

Country Status (7)

Country Link
AU (2) AU527165B2 (en)
BR (1) BR7807290A (en)
FR (3) FR2422717B1 (en)
LU (1) LU80489A1 (en)
OA (1) OA06002A (en)
RO (1) RO80052B (en)
ZM (1) ZM9578A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
US4645828A (en) * 1984-03-23 1987-02-24 Oncogen Platelet related growth regulator
AU580064B2 (en) * 1984-06-04 1988-12-22 Lubrizol Genetics Inc. Nitrogen fixation regulator genes
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
US4987070A (en) * 1987-03-04 1991-01-22 Suntory Limited Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment
NZ187300A (en) * 1977-05-27 1982-08-17 Univ California Dna transfer vector and micro-organism modified to contain a nucleotide sequence equivalent to the gene of a higher organism
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
BR7807288A (en) * 1977-11-08 1979-06-12 Genentech Inc POLYNUCLEOTIDE SYNTHESIS PROCESS
FR2422685A1 (en) * 1978-04-14 1979-11-09 Pasteur Institut VECTORS, DERIVED FROM LAMBDA BACTERIOPHAGUS, ALLOWING THE INSERTION AND EXPRESSION OF A GENE, PROCARYOTE OR EUKARYOTE, UNDER THE CONTROL OF THE PROMOTER AND THE OPERATOR OF THE LACTOSE OPERON OF ESCHERICHIA COLI BACTERIA
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
FR2428075A1 (en) * 1978-06-08 1980-01-04 Pasteur Institut PROCESS FOR THE PRODUCTION OF PROTEINS BY EXPRESSION OF THE CORRESPONDING GENES IN BACTERIA AND VECTORS LIKELY TO BE USED IN SUCH METHODS
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
GB2033905B (en) * 1978-08-21 1982-10-13 Upjohn Co Bacterial preparation of proteins

Also Published As

Publication number Publication date
FR2422718A1 (en) 1979-11-09
OA06002A (en) 1981-06-30
AU4136178A (en) 1979-05-17
LU80489A1 (en) 1979-03-22
AU527165B2 (en) 1983-02-17
FR2422717A1 (en) 1979-11-09
AU7448481A (en) 1982-01-14
FR2422719A1 (en) 1979-11-09
FR2422718B1 (en) 1984-05-18
AU546092B2 (en) 1985-08-15
FR2422719B1 (en) 1984-12-28
BR7807290A (en) 1979-06-12
FR2422717B1 (en) 1985-12-06
RO80052A (en) 1985-03-15
ZM9578A1 (en) 1980-03-21

Similar Documents

Publication Publication Date Title
Zipursky et al. Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
Gaillard et al. Ethanol precipitation of DNA with linear polyacrylamide as carrier
Jack et al. Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.
DE3588255D1 (en) Cloning and expression of HTLV-III DNA
Westhoff et al. Genes and transcripts for the P700 chlorophyll a apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes
IL75508A (en) Expression vectors for desulphatohirudin,host cells transformed therewith and the production of desulphatohirudin by means of these host cells
FI853056A0 (en) Process for the preparation of polypeptide with hirudin activity
IL76762A (en) Autonomous replication sequences for yeast strains of the species pichia pastoris
EP0174367A4 (en) QUANTITY AND METHOD FOR PRODUCING RECOMBINED PRODUCTS IN LARGE YIELD.
LV5288A3 (en) The Nucleic Fragment is a code for human proapolipoprotein A-1
UA32548C2 (en) Isolated DNA sequence coding EPSPS ferment, Isolated DNA sequence coding protein, revealING EPSPS activity, recombinant bifilar DNA molecule, a method for producing genetically transformed plants
ATE482714T1 (en) MODIFIED HEAT SHOCK PROTEIN-ANTIGEN PEPTIDE COMPLEX
Hirashima et al. Purification and properties of ribosome-releasing factor
EP0095350B1 (en) A method of producing human gamma-interferon-like polipeptide
CN101920009B (en) Vaccine for preventing and curing tumor
DE3062792D1 (en) A process in purification and concentration of the factor viii-complex
Nazar et al. Nucleotide sequence of Halobacterium cutirubrum ribosomal 5 S ribonucleic acid. An altered secondary structure in halophilic organisms
Lanzendörfer et al. Structure and function of the DNA-dependent RNA polymerase of Sulfolobus
FR2634495B1 (en) PROCESS FOR THE PREPARATION OF A PROTEIN BY YEASTS USING AN INDUCTIBLE SYSTEM, CORRESPONDING VECTORS AND STRAINS
EP0094797A3 (en) Novel cloning vehicles for polypeptide expression in microbial hosts
HUP0004258A2 (en) Processes for increasing the yield in plants
CN104140972B (en) The preparation method of diphtheria toxin muton CRM 197
Isono et al. Purification and Characterization of 30‐S Ribosomal Proteins from Bacillus stearothermophilus
ZA81292B (en) Microbiologically prepared polypeptide with the aminoacid sequence of the proinsulin or primates,dna and plasmids which act as codings for this sequence,microorganisms which contain this genetic information,and processes for their preparation
Christiansen et al. Cloning and sequencing of two genes from Staphylococcus carnosus coding for glucose-specific PTS and their expression in Escherichia coli K-12