DE69011183T2 - Hefestämme für die Herstellung von heterologen reifen Proteinen, besonders von Hirudin. - Google Patents

Hefestämme für die Herstellung von heterologen reifen Proteinen, besonders von Hirudin.

Info

Publication number
DE69011183T2
DE69011183T2 DE69011183T DE69011183T DE69011183T2 DE 69011183 T2 DE69011183 T2 DE 69011183T2 DE 69011183 T DE69011183 T DE 69011183T DE 69011183 T DE69011183 T DE 69011183T DE 69011183 T2 DE69011183 T2 DE 69011183T2
Authority
DE
Germany
Prior art keywords
heterologous
production
yeast strains
mature proteins
hirudin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69011183T
Other languages
English (en)
Other versions
DE69011183D1 (de
Inventor
Yves Lemoine
Martine Nguyen
Tilman Achstetter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Application granted granted Critical
Publication of DE69011183D1 publication Critical patent/DE69011183D1/de
Publication of DE69011183T2 publication Critical patent/DE69011183T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
DE69011183T 1989-03-31 1990-03-28 Hefestämme für die Herstellung von heterologen reifen Proteinen, besonders von Hirudin. Expired - Fee Related DE69011183T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8904305A FR2645174B1 (fr) 1989-03-31 1989-03-31 Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant

Publications (2)

Publication Number Publication Date
DE69011183D1 DE69011183D1 (de) 1994-09-08
DE69011183T2 true DE69011183T2 (de) 1995-03-16

Family

ID=9380295

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69011183T Expired - Fee Related DE69011183T2 (de) 1989-03-31 1990-03-28 Hefestämme für die Herstellung von heterologen reifen Proteinen, besonders von Hirudin.

Country Status (10)

Country Link
US (1) US5521093A (de)
EP (1) EP0396436B1 (de)
JP (1) JP3135550B2 (de)
AT (1) ATE109506T1 (de)
CA (1) CA2013239C (de)
DE (1) DE69011183T2 (de)
DK (1) DK0396436T3 (de)
ES (1) ES2060974T3 (de)
FR (1) FR2645174B1 (de)
PT (1) PT93614B (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
JP2000514288A (ja) * 1996-06-28 2000-10-31 ノボ ノルディスク アクティーゼルスカブ ムタナーゼ活性を有する組換え酵素
US6066070A (en) * 1998-04-28 2000-05-23 Toyota Jidosha Kabushiki Kaisha Control system of vehicle having continuously variable transmission
JP3546302B2 (ja) 1999-08-05 2004-07-28 トヨタ自動車株式会社 無段変速機を備えた車両の制御装置
JP3539335B2 (ja) 2000-03-10 2004-07-07 トヨタ自動車株式会社 無段変速機を備えた車両の制御装置
RU2663587C2 (ru) 2013-03-06 2018-08-07 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Клетки-хозяева и способы использования
US9388599B2 (en) 2014-02-27 2016-07-12 Parsons Corporation Wind tower erection system
CN106337042B (zh) * 2015-07-16 2019-08-09 上海雅心生物技术有限公司 双碱基酶kex2变体及其高效表达方法
EP3604158B1 (de) 2017-03-31 2024-05-01 Nissei Asb Machine Co., Ltd. Griff und behälter mit dem griff
CN113913414B (zh) * 2021-05-28 2023-09-26 上海雅心生物技术有限公司 高稳定性和高催化效率的双碱基酶Kex2突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236183A (ja) * 1985-06-20 1987-02-17 ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド サツカロミセス・セレビシエからのポリペプチドの発現および分泌
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
US5179003A (en) * 1988-01-05 1993-01-12 Boehringer Mannheim Gmbh Process for the production of proteins or protein-containing gene products
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
FR2634495B1 (fr) * 1988-07-01 1990-10-26 Chambon Pierre Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes

Also Published As

Publication number Publication date
JP3135550B2 (ja) 2001-02-19
PT93614B (pt) 1996-08-30
CA2013239C (fr) 2000-01-18
PT93614A (pt) 1990-11-20
EP0396436A1 (de) 1990-11-07
JPH0372867A (ja) 1991-03-28
FR2645174B1 (fr) 1994-01-07
DK0396436T3 (da) 1994-12-05
FR2645174A1 (fr) 1990-10-05
ATE109506T1 (de) 1994-08-15
DE69011183D1 (de) 1994-09-08
CA2013239A1 (fr) 1990-09-30
ES2060974T3 (es) 1994-12-01
US5521093A (en) 1996-05-28
EP0396436B1 (de) 1994-08-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee