ES2060974T3 - Cepas de levadura para la produccion de proteinas heterologas maduras, en particular de hirudina. - Google Patents

Cepas de levadura para la produccion de proteinas heterologas maduras, en particular de hirudina.

Info

Publication number
ES2060974T3
ES2060974T3 ES90400838T ES90400838T ES2060974T3 ES 2060974 T3 ES2060974 T3 ES 2060974T3 ES 90400838 T ES90400838 T ES 90400838T ES 90400838 T ES90400838 T ES 90400838T ES 2060974 T3 ES2060974 T3 ES 2060974T3
Authority
ES
Spain
Prior art keywords
hirudine
mature
production
heterological
yeast strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90400838T
Other languages
English (en)
Inventor
Yves Lemoine
Martine Nguyen
Tilman Achstetter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Application granted granted Critical
Publication of ES2060974T3 publication Critical patent/ES2060974T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast

Abstract

LA PRESENTE INVENCION SE REFIERE A UN SISTEMA MEJORADO PARA LA PRODUCCION DE PROTEINAS HETEROLOGAS MADURAS, Y EN PARTICULAR DE HIRUDINA, POR LEVADURAS QUE CONTIENEN UN VECTOR DE EXPRESION QUE CONTIENE UNA SECUENCIA CODIFICANTE PARA LA PROTEINA HETEROLOGA. ESTA MEJORA SE CARACTERIZA POR LA AMPLIACION DEL GEN KEX2 DE LEVADURA, QUE CODIFICA PARA LA ENDOPROTEASA YSCF. LA AMPLIACION SE EFECTUA POR INTEGRACION DE UNA O VARIAS COPIAS DE TODO O PARTE DEL GEN KEX2 EN EL GENOMA DE LA LEVADURA, O POR INSERCION DE UNA O VARIAS COPIAS DE TODO O PARTE DEL GEN KEX2 EN EL VECTOR DE EXPRESION DE LA PROTEINA HETEROLOGA.
ES90400838T 1989-03-31 1990-03-28 Cepas de levadura para la produccion de proteinas heterologas maduras, en particular de hirudina. Expired - Lifetime ES2060974T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8904305A FR2645174B1 (fr) 1989-03-31 1989-03-31 Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant

Publications (1)

Publication Number Publication Date
ES2060974T3 true ES2060974T3 (es) 1994-12-01

Family

ID=9380295

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90400838T Expired - Lifetime ES2060974T3 (es) 1989-03-31 1990-03-28 Cepas de levadura para la produccion de proteinas heterologas maduras, en particular de hirudina.

Country Status (10)

Country Link
US (1) US5521093A (es)
EP (1) EP0396436B1 (es)
JP (1) JP3135550B2 (es)
AT (1) ATE109506T1 (es)
CA (1) CA2013239C (es)
DE (1) DE69011183T2 (es)
DK (1) DK0396436T3 (es)
ES (1) ES2060974T3 (es)
FR (1) FR2645174B1 (es)
PT (1) PT93614B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
AU721693B2 (en) * 1996-06-28 2000-07-13 Novozymes A/S A recombinant enzyme with mutanase activity
US6066070A (en) * 1998-04-28 2000-05-23 Toyota Jidosha Kabushiki Kaisha Control system of vehicle having continuously variable transmission
JP3546302B2 (ja) 1999-08-05 2004-07-28 トヨタ自動車株式会社 無段変速機を備えた車両の制御装置
JP3539335B2 (ja) 2000-03-10 2004-07-07 トヨタ自動車株式会社 無段変速機を備えた車両の制御装置
WO2014138371A1 (en) 2013-03-06 2014-09-12 Glaxosmithkline Llc Host cells and methods of use
US9388599B2 (en) 2014-02-27 2016-07-12 Parsons Corporation Wind tower erection system
CN106337042B (zh) * 2015-07-16 2019-08-09 上海雅心生物技术有限公司 双碱基酶kex2变体及其高效表达方法
CN110475726B (zh) 2017-03-31 2021-04-20 日精Asb机械株式会社 把手和具备把手的容器
CN113913414B (zh) * 2021-05-28 2023-09-26 上海雅心生物技术有限公司 高稳定性和高催化效率的双碱基酶Kex2突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5912586A (en) * 1985-06-20 1986-12-24 Salk Institute Biotechnology/Industrial Associates, Inc., The Alpha-factor leader peptide-facilitated secretion from transformed s cerevisiae
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
EP0327797B1 (de) * 1988-01-05 2003-09-03 Roche Diagnostics GmbH Verfahren zur Herstellung von Proteinen oder proteinhaltigen Genprodukten
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
FR2634495B1 (fr) * 1988-07-01 1990-10-26 Chambon Pierre Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes

Also Published As

Publication number Publication date
JP3135550B2 (ja) 2001-02-19
DK0396436T3 (da) 1994-12-05
ATE109506T1 (de) 1994-08-15
JPH0372867A (ja) 1991-03-28
EP0396436A1 (fr) 1990-11-07
US5521093A (en) 1996-05-28
CA2013239A1 (fr) 1990-09-30
PT93614A (pt) 1990-11-20
PT93614B (pt) 1996-08-30
FR2645174B1 (fr) 1994-01-07
DE69011183D1 (de) 1994-09-08
CA2013239C (fr) 2000-01-18
EP0396436B1 (fr) 1994-08-03
FR2645174A1 (fr) 1990-10-05
DE69011183T2 (de) 1995-03-16

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