DE3587205T2 - Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle. - Google Patents

Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle.

Info

Publication number
DE3587205T2
DE3587205T2 DE19853587205 DE3587205T DE3587205T2 DE 3587205 T2 DE3587205 T2 DE 3587205T2 DE 19853587205 DE19853587205 DE 19853587205 DE 3587205 T DE3587205 T DE 3587205T DE 3587205 T2 DE3587205 T2 DE 3587205T2
Authority
DE
Germany
Prior art keywords
vector
protein
producing
recombinant dna
transformed cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE19853587205
Other languages
English (en)
Other versions
DE3587205D1 (de
Inventor
Tetsuo Miyake
Takanori Oka
Gakuzo Tamura
Makari Yamasaki
Koji Yoda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wakunaga Pharmaceutical Co Ltd
Original Assignee
Wakunaga Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP59159703A external-priority patent/JP2524693B2/ja
Priority claimed from JP59279585A external-priority patent/JP2524695B2/ja
Application filed by Wakunaga Pharmaceutical Co Ltd filed Critical Wakunaga Pharmaceutical Co Ltd
Application granted granted Critical
Publication of DE3587205D1 publication Critical patent/DE3587205D1/de
Publication of DE3587205T2 publication Critical patent/DE3587205T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF] (urogastrone)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
DE19853587205 1984-07-30 1985-07-30 Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle. Expired - Fee Related DE3587205T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP59159703A JP2524693B2 (ja) 1984-07-30 1984-07-30 蛋白質の製造法
JP59279585A JP2524695B2 (ja) 1984-12-26 1984-12-26 蛋白質の製造法

Publications (2)

Publication Number Publication Date
DE3587205D1 DE3587205D1 (de) 1993-04-29
DE3587205T2 true DE3587205T2 (de) 1993-08-26

Family

ID=26486416

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19853587205 Expired - Fee Related DE3587205T2 (de) 1984-07-30 1985-07-30 Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle.

Country Status (2)

Country Link
EP (1) EP0170266B1 (de)
DE (1) DE3587205T2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3436818A1 (de) * 1984-10-06 1986-04-10 Hoechst Ag, 6230 Frankfurt Synthetische signalsequenz zum transport von proteinen in expressionssystemen
JP2549504B2 (ja) * 1984-12-21 1996-10-30 ア−ス製薬株式会社 Ｄｎａ塩基配列、ポリペプチド分泌発現ベクター及び形質転換微生物
US4935352A (en) * 1985-10-21 1990-06-19 Takeda Chemical Industries, Ltd. Expression vector for animal cell line and use thereof
JP2574146B2 (ja) * 1986-02-14 1997-01-22 アース製薬 株式会社 ポリペプチド発現ベクタ−、該ベクタ−で形質転換した宿主及び該宿主によるポリペプチドの製造法
TW205070B (de) * 1986-06-30 1993-05-01 Takeda Pharm Industry Co Ltd
ES2081801T3 (es) * 1987-10-30 1996-03-16 Bristol Myers Squibb Co Plasmidos de expresion.
US5011772A (en) * 1988-02-05 1991-04-30 Public Health Research Institute Of The City Of N.Y. High yield protein production system
FR2636643B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
DK582381A (da) * 1981-01-02 1982-07-03 Univ New York Remkombinat plasmid til anvendelse ved bakteriel kloning
JPS5869897A (ja) * 1981-10-20 1983-04-26 Gakuzo Tamura Ｄｎａ遺伝子
DE3382547D1 (de) * 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
JPS6030687A (ja) * 1983-08-01 1985-02-16 Wakunaga Seiyaku Kk Ｄνａ遺伝子，その製造法およびそれを含むプラスミド
EP0188555A4 (de) * 1984-07-09 1988-04-18 Integrated Genetics Inc Klonierungsvektor für hefe.

Also Published As

Publication number Publication date
EP0170266B1 (de) 1993-03-24
EP0170266A3 (en) 1988-01-13
DE3587205D1 (de) 1993-04-29
EP0170266A2 (de) 1986-02-05

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee