SK18862001A3 - Gény Corynebacterium glutamicum kódujúce proteíny metabolických dráh - Google Patents

Info

Publication number

SK18862001A3

SK18862001A3 SK1886-2001A SK18862001A SK18862001A3 SK 18862001 A3 SK18862001 A3 SK 18862001A3 SK 18862001 A SK18862001 A SK 18862001A SK 18862001 A3 SK18862001 A3 SK 18862001A3

Authority

SK

Slovakia

Prior art keywords

nucleic acid

protein

sequence

acid molecule

amino acid

Prior art date

1999-06-25

Links

• Espacenet
• Global Dossier
• Discuss

• 108090000623 proteins and genes Proteins 0.000 title claims abstract description 451
• 102000004169 proteins and genes Human genes 0.000 title claims abstract description 329
• 241000186226 Corynebacterium glutamicum Species 0.000 title claims abstract description 254
• 230000037353 metabolic pathway Effects 0.000 title claims description 302
• 150000007523 nucleic acids Chemical class 0.000 claims abstract description 257
• 102000039446 nucleic acids Human genes 0.000 claims abstract description 237
• 108020004707 nucleic acids Proteins 0.000 claims abstract description 237
• 238000000034 method Methods 0.000 claims abstract description 116
• 238000004519 manufacturing process Methods 0.000 claims abstract description 75
• 239000013604 expression vector Substances 0.000 claims abstract description 45
• 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 45
• 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 40
• 235000018102 proteins Nutrition 0.000 claims description 318
• 125000003729 nucleotide group Chemical group 0.000 claims description 144
• 239000002773 nucleotide Substances 0.000 claims description 142
• 150000001413 amino acids Chemical class 0.000 claims description 113
• 235000001014 amino acid Nutrition 0.000 claims description 108
• 229940024606 amino acid Drugs 0.000 claims description 103
• 230000000694 effects Effects 0.000 claims description 98
• 239000000126 substance Substances 0.000 claims description 80
• 239000013598 vector Substances 0.000 claims description 72
• 102000004190 Enzymes Human genes 0.000 claims description 66
• 108090000790 Enzymes Proteins 0.000 claims description 66
• 230000014509 gene expression Effects 0.000 claims description 63
• 229940088594 vitamin Drugs 0.000 claims description 48
• 229930003231 vitamin Natural products 0.000 claims description 48
• 235000013343 vitamin Nutrition 0.000 claims description 48
• 239000011782 vitamin Substances 0.000 claims description 48
• 241000186216 Corynebacterium Species 0.000 claims description 47
• 239000002777 nucleoside Substances 0.000 claims description 41
• 244000005700 microbiome Species 0.000 claims description 39
• HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 36
• HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 36
• HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 36
• 239000002417 nutraceutical Substances 0.000 claims description 36
• 235000021436 nutraceutical agent Nutrition 0.000 claims description 36
• 229920001184 polypeptide Polymers 0.000 claims description 33
• 230000001105 regulatory effect Effects 0.000 claims description 33
• 241000186146 Brevibacterium Species 0.000 claims description 26
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 23
• 150000003722 vitamin derivatives Chemical class 0.000 claims description 23
• 125000003835 nucleoside group Chemical group 0.000 claims description 21
• KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 20
• 230000004060 metabolic process Effects 0.000 claims description 20
• 150000003833 nucleoside derivatives Chemical class 0.000 claims description 20
• 239000004472 Lysine Substances 0.000 claims description 19
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 17
• 241000319304 [Brevibacterium] flavum Species 0.000 claims description 17
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 16
• 241000186227 Corynebacterium diphtheriae Species 0.000 claims description 14
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 12
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 11
• 229960002429 proline Drugs 0.000 claims description 11
• QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 10
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 10
• -1 cofactors Natural products 0.000 claims description 10
• 150000001720 carbohydrates Chemical class 0.000 claims description 9
• 235000014633 carbohydrates Nutrition 0.000 claims description 9
• 239000004475 Arginine Substances 0.000 claims description 8
• 239000004471 Glycine Substances 0.000 claims description 8
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 8
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 8
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 8
• CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims description 8
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 8
• 239000004473 Threonine Substances 0.000 claims description 8
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 8
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 8
• 229960003121 arginine Drugs 0.000 claims description 8
• 229960002433 cysteine Drugs 0.000 claims description 8
• 239000001963 growth medium Substances 0.000 claims description 8
• 229960000310 isoleucine Drugs 0.000 claims description 8
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 8
• 229960002898 threonine Drugs 0.000 claims description 8
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 7
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 7
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 7
• 239000002585 base Substances 0.000 claims description 7
• 238000012258 culturing Methods 0.000 claims description 7
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 7
• 235000018417 cysteine Nutrition 0.000 claims description 7
• 229940049906 glutamate Drugs 0.000 claims description 7
• 229930195712 glutamate Natural products 0.000 claims description 7
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 7
• 229960002885 histidine Drugs 0.000 claims description 7
• 150000002632 lipids Chemical class 0.000 claims description 7
• 150000007524 organic acids Chemical class 0.000 claims description 7
• 229960005190 phenylalanine Drugs 0.000 claims description 7
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 7
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 6
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 6
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 6
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 6
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 6
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 6
• 229960003767 alanine Drugs 0.000 claims description 6
• 229960003136 leucine Drugs 0.000 claims description 6
• 230000004048 modification Effects 0.000 claims description 6
• 238000012986 modification Methods 0.000 claims description 6
• 235000005985 organic acids Nutrition 0.000 claims description 6
• 229960004295 valine Drugs 0.000 claims description 6
• 239000004474 valine Substances 0.000 claims description 6
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 5
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 5
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 5
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 5
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 5
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 5
• 235000004279 alanine Nutrition 0.000 claims description 5
• 229940009098 aspartate Drugs 0.000 claims description 5
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 5
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 5
• 235000012539 Bacterium linens Nutrition 0.000 claims description 4
• 241000187561 Rhodococcus erythropolis Species 0.000 claims description 4
• 229910021529 ammonia Inorganic materials 0.000 claims description 4
• 150000001491 aromatic compounds Chemical class 0.000 claims description 4
• 150000002009 diols Chemical class 0.000 claims description 4
• 229930182817 methionine Natural products 0.000 claims description 4
• 241001517047 Corynebacterium acetoacidophilum Species 0.000 claims description 3
• 229930001119 polyketide Natural products 0.000 claims description 3
• 125000000830 polyketide group Chemical group 0.000 claims description 3
• 229920006395 saturated elastomer Polymers 0.000 claims description 3
• 150000004671 saturated fatty acids Chemical class 0.000 claims description 3
• 235000003441 saturated fatty acids Nutrition 0.000 claims description 3
• 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 3
• 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 3
• 241000186310 Brevibacterium linens Species 0.000 claims 1
• 150000001875 compounds Chemical class 0.000 abstract description 61
• 102000037865 fusion proteins Human genes 0.000 abstract description 27
• 108020001507 fusion proteins Proteins 0.000 abstract description 27
• 238000003259 recombinant expression Methods 0.000 abstract description 14
• 230000000692 anti-sense effect Effects 0.000 abstract description 2
• 230000006872 improvement Effects 0.000 abstract description 2
• 230000000890 antigenic effect Effects 0.000 abstract 1
• 238000010353 genetic engineering Methods 0.000 abstract 1
• 210000004027 cell Anatomy 0.000 description 136
• 125000003275 alpha amino acid group Chemical group 0.000 description 83
• 108020004414 DNA Proteins 0.000 description 67
• 229940088598 enzyme Drugs 0.000 description 64
• 230000015572 biosynthetic process Effects 0.000 description 48
• 108091028043 Nucleic acid sequence Proteins 0.000 description 36
• 239000012634 fragment Substances 0.000 description 33
• 101150031278 MP gene Proteins 0.000 description 32
• 230000001965 increasing effect Effects 0.000 description 29
• 238000010367 cloning Methods 0.000 description 27
• 238000004458 analytical method Methods 0.000 description 26
• 239000000543 intermediate Substances 0.000 description 26
• 241000894006 Bacteria Species 0.000 description 25
• 108091026890 Coding region Proteins 0.000 description 25
• 239000000047 product Substances 0.000 description 25
• 239000013612 plasmid Substances 0.000 description 24
• 230000006870 function Effects 0.000 description 22
• 230000006696 biosynthetic metabolic pathway Effects 0.000 description 20
• 108020004999 messenger RNA Proteins 0.000 description 20
• 230000001580 bacterial effect Effects 0.000 description 19
• 230000015556 catabolic process Effects 0.000 description 19
• 238000006731 degradation reaction Methods 0.000 description 19
• 239000000523 sample Substances 0.000 description 19
• 238000003786 synthesis reaction Methods 0.000 description 19
• 125000000539 amino acid group Chemical group 0.000 description 17
• 230000001413 cellular effect Effects 0.000 description 17
• 241000588724 Escherichia coli Species 0.000 description 16
• 238000006911 enzymatic reaction Methods 0.000 description 16
• 238000007479 molecular analysis Methods 0.000 description 16
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 15
• 241000186145 Corynebacterium ammoniagenes Species 0.000 description 15
• 235000018977 lysine Nutrition 0.000 description 15
• 239000002609 medium Substances 0.000 description 15
• 230000035772 mutation Effects 0.000 description 15
• 230000000295 complement effect Effects 0.000 description 14
• 238000000855 fermentation Methods 0.000 description 14
• 230000004151 fermentation Effects 0.000 description 14
• 230000004927 fusion Effects 0.000 description 14
• 238000010561 standard procedure Methods 0.000 description 14
• 238000002703 mutagenesis Methods 0.000 description 13
• 231100000350 mutagenesis Toxicity 0.000 description 13
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
• 230000001851 biosynthetic effect Effects 0.000 description 12
• 238000012512 characterization method Methods 0.000 description 12
• 238000006243 chemical reaction Methods 0.000 description 12
• 238000009396 hybridization Methods 0.000 description 12
• 230000037361 pathway Effects 0.000 description 12
• 239000002243 precursor Substances 0.000 description 12
• 230000014616 translation Effects 0.000 description 12
• 102000053602 DNA Human genes 0.000 description 11
• 108091034117 Oligonucleotide Proteins 0.000 description 11
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 11
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 11
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 11
• 239000000203 mixture Substances 0.000 description 11
• 238000000746 purification Methods 0.000 description 11
• 238000013518 transcription Methods 0.000 description 11
• 230000035897 transcription Effects 0.000 description 11
• 230000009466 transformation Effects 0.000 description 11
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
• GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 10
• 241000196324 Embryophyta Species 0.000 description 10
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 10
• 238000010276 construction Methods 0.000 description 10
• 238000012217 deletion Methods 0.000 description 10
• 230000037430 deletion Effects 0.000 description 10
• 239000013615 primer Substances 0.000 description 10
• 241000233866 Fungi Species 0.000 description 9
• AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 9
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
• 230000027455 binding Effects 0.000 description 9
• 229910052799 carbon Inorganic materials 0.000 description 9
• 230000006652 catabolic pathway Effects 0.000 description 9
• 239000012707 chemical precursor Substances 0.000 description 9
• 238000005516 engineering process Methods 0.000 description 9
• 230000012010 growth Effects 0.000 description 9
• 238000002744 homologous recombination Methods 0.000 description 9
• 230000006801 homologous recombination Effects 0.000 description 9
• 239000011713 pantothenic acid Substances 0.000 description 9
• 235000002639 sodium chloride Nutrition 0.000 description 9
• 241000894007 species Species 0.000 description 9
• 238000006467 substitution reaction Methods 0.000 description 9
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
• 238000012300 Sequence Analysis Methods 0.000 description 8
• OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 8
• 230000010354 integration Effects 0.000 description 8
• 239000011159 matrix material Substances 0.000 description 8
• 235000019161 pantothenic acid Nutrition 0.000 description 8
• 230000008569 process Effects 0.000 description 8
• 238000011160 research Methods 0.000 description 8
• 238000012216 screening Methods 0.000 description 8
• 238000012163 sequencing technique Methods 0.000 description 8
• 239000000758 substrate Substances 0.000 description 8
• 108090000994 Catalytic RNA Proteins 0.000 description 7
• 102000053642 Catalytic RNA Human genes 0.000 description 7
• 238000000018 DNA microarray Methods 0.000 description 7
• 101100245038 Synechocystis sp. (strain PCC 6803 / Kazusa) proA1 gene Proteins 0.000 description 7
• 238000007792 addition Methods 0.000 description 7
• 235000009697 arginine Nutrition 0.000 description 7
• 239000000872 buffer Substances 0.000 description 7
• 239000003795 chemical substances by application Substances 0.000 description 7
• 230000000875 corresponding effect Effects 0.000 description 7
• 238000001962 electrophoresis Methods 0.000 description 7
• 210000003527 eukaryotic cell Anatomy 0.000 description 7
• 230000002068 genetic effect Effects 0.000 description 7
• 238000003780 insertion Methods 0.000 description 7
• 230000037431 insertion Effects 0.000 description 7
• 239000000463 material Substances 0.000 description 7
• 229940014662 pantothenate Drugs 0.000 description 7
• 238000002360 preparation method Methods 0.000 description 7
• 101150118057 proA gene Proteins 0.000 description 7
• 210000001236 prokaryotic cell Anatomy 0.000 description 7
• 230000004952 protein activity Effects 0.000 description 7
• 108091092562 ribozyme Proteins 0.000 description 7
• JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 7
• 238000012546 transfer Methods 0.000 description 7
• 238000013519 translation Methods 0.000 description 7
• ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 6
• 239000003155 DNA primer Substances 0.000 description 6
• 108010021466 Mutant Proteins Proteins 0.000 description 6
• 102000008300 Mutant Proteins Human genes 0.000 description 6
• PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 6
• 108091005461 Nucleic proteins Proteins 0.000 description 6
• 229910019142 PO4 Inorganic materials 0.000 description 6
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
• 239000002253 acid Substances 0.000 description 6
• KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
• 239000000499 gel Substances 0.000 description 6
• 229960004452 methionine Drugs 0.000 description 6
• 229910052757 nitrogen Inorganic materials 0.000 description 6
• 235000016709 nutrition Nutrition 0.000 description 6
• 230000002829 reductive effect Effects 0.000 description 6
• 239000011347 resin Substances 0.000 description 6
• 229920005989 resin Polymers 0.000 description 6
• 238000012552 review Methods 0.000 description 6
• 238000000926 separation method Methods 0.000 description 6
• 230000032258 transport Effects 0.000 description 6
• 238000011144 upstream manufacturing Methods 0.000 description 6
• 241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 description 5
• 108010070675 Glutathione transferase Proteins 0.000 description 5
• 102000005720 Glutathione transferase Human genes 0.000 description 5
• XKMLYUALXHKNFT-UUOKFMHZSA-N Guanosine-5'-triphosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XKMLYUALXHKNFT-UUOKFMHZSA-N 0.000 description 5
• 241000238631 Hexapoda Species 0.000 description 5
• 241001465754 Metazoa Species 0.000 description 5
• 239000007984 Tris EDTA buffer Substances 0.000 description 5
• 238000003556 assay Methods 0.000 description 5
• 229960002685 biotin Drugs 0.000 description 5
• 235000020958 biotin Nutrition 0.000 description 5
• 239000011616 biotin Substances 0.000 description 5
• 229940041514 candida albicans extract Drugs 0.000 description 5
• 238000005119 centrifugation Methods 0.000 description 5
• 239000002537 cosmetic Substances 0.000 description 5
• 238000011161 development Methods 0.000 description 5
• 201000010099 disease Diseases 0.000 description 5
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
• 238000002474 experimental method Methods 0.000 description 5
• 235000019152 folic acid Nutrition 0.000 description 5
• 239000011724 folic acid Substances 0.000 description 5
• 235000013305 food Nutrition 0.000 description 5
• 239000011521 glass Substances 0.000 description 5
• 238000000338 in vitro Methods 0.000 description 5
• 230000002401 inhibitory effect Effects 0.000 description 5
• 230000005764 inhibitory process Effects 0.000 description 5
• 230000000670 limiting effect Effects 0.000 description 5
• 210000004962 mammalian cell Anatomy 0.000 description 5
• 230000001404 mediated effect Effects 0.000 description 5
• 238000010369 molecular cloning Methods 0.000 description 5
• 239000010452 phosphate Substances 0.000 description 5
• 230000004144 purine metabolism Effects 0.000 description 5
• XKMLYUALXHKNFT-UHFFFAOYSA-N rGTP Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O XKMLYUALXHKNFT-UHFFFAOYSA-N 0.000 description 5
• 101150079601 recA gene Proteins 0.000 description 5
• 239000011780 sodium chloride Substances 0.000 description 5
• 210000001519 tissue Anatomy 0.000 description 5
• 230000003612 virological effect Effects 0.000 description 5
• 239000012138 yeast extract Substances 0.000 description 5
• UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 4
• 108010078791 Carrier Proteins Proteins 0.000 description 4
• 108091033380 Coding strand Proteins 0.000 description 4
• AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 4
• 230000006820 DNA synthesis Effects 0.000 description 4
• UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 4
• 102000003960 Ligases Human genes 0.000 description 4
• 108090000364 Ligases Proteins 0.000 description 4
• NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
• 125000003118 aryl group Chemical group 0.000 description 4
• 230000008859 change Effects 0.000 description 4
• 239000005515 coenzyme Substances 0.000 description 4
• 239000002299 complementary DNA Substances 0.000 description 4
• 101150000582 dapE gene Proteins 0.000 description 4
• 239000003623 enhancer Substances 0.000 description 4
• 235000020776 essential amino acid Nutrition 0.000 description 4
• 239000003797 essential amino acid Substances 0.000 description 4
• 238000004128 high performance liquid chromatography Methods 0.000 description 4
• 238000011534 incubation Methods 0.000 description 4
• 230000003993 interaction Effects 0.000 description 4
• 238000002372 labelling Methods 0.000 description 4
• 101150033534 lysA gene Proteins 0.000 description 4
• 238000013507 mapping Methods 0.000 description 4
• 239000003550 marker Substances 0.000 description 4
• 230000007246 mechanism Effects 0.000 description 4
• 230000002503 metabolic effect Effects 0.000 description 4
• 235000001968 nicotinic acid Nutrition 0.000 description 4
• 229960003512 nicotinic acid Drugs 0.000 description 4
• 239000011664 nicotinic acid Substances 0.000 description 4
• 230000035764 nutrition Effects 0.000 description 4
• 238000003752 polymerase chain reaction Methods 0.000 description 4
• 239000002987 primer (paints) Substances 0.000 description 4
• 238000001243 protein synthesis Methods 0.000 description 4
• 150000003212 purines Chemical class 0.000 description 4
• LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 4
• 238000010188 recombinant method Methods 0.000 description 4
• 230000010076 replication Effects 0.000 description 4
• 230000000717 retained effect Effects 0.000 description 4
• 235000019192 riboflavin Nutrition 0.000 description 4
• 239000002151 riboflavin Substances 0.000 description 4
• 229960002477 riboflavin Drugs 0.000 description 4
• 150000003839 salts Chemical class 0.000 description 4
• 239000013605 shuttle vector Substances 0.000 description 4
• OTOIIPJYVQJATP-BYPYZUCNSA-N (R)-pantoic acid Chemical compound OCC(C)(C)[C@@H](O)C(O)=O OTOIIPJYVQJATP-BYPYZUCNSA-N 0.000 description 3
• RQFCJASXJCIDSX-UHFFFAOYSA-N 14C-Guanosin-5'-monophosphat Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(O)=O)C(O)C1O RQFCJASXJCIDSX-UHFFFAOYSA-N 0.000 description 3
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 3
• 244000177578 Bacterium linens Species 0.000 description 3
• 102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 3
• RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical group O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 3
• UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 3
• 102000052510 DNA-Binding Proteins Human genes 0.000 description 3
• 102100031780 Endonuclease Human genes 0.000 description 3
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
• 108010073324 Glutaminase Proteins 0.000 description 3
• 102000009127 Glutaminase Human genes 0.000 description 3
• 241000282412 Homo Species 0.000 description 3
• 235000019766 L-Lysine Nutrition 0.000 description 3
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 3
• 108020004687 Malate Synthase Proteins 0.000 description 3
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
• OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 3
• 108091092724 Noncoding DNA Proteins 0.000 description 3
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
• 241001591005 Siga Species 0.000 description 3
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
• 229930006000 Sucrose Natural products 0.000 description 3
• DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 description 3
• UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 3
• 229950006790 adenosine phosphate Drugs 0.000 description 3
• 230000037354 amino acid metabolism Effects 0.000 description 3
• 229960004050 aminobenzoic acid Drugs 0.000 description 3
• 229960001230 asparagine Drugs 0.000 description 3
• 235000009582 asparagine Nutrition 0.000 description 3
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
• 229960003237 betaine Drugs 0.000 description 3
• 230000008238 biochemical pathway Effects 0.000 description 3
• 230000033228 biological regulation Effects 0.000 description 3
• 239000006227 byproduct Substances 0.000 description 3
• 210000004899 c-terminal region Anatomy 0.000 description 3
• 239000004202 carbamide Substances 0.000 description 3
• FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 3
• RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 3
• 239000005516 coenzyme A Substances 0.000 description 3
• 229940093530 coenzyme a Drugs 0.000 description 3
• 230000002860 competitive effect Effects 0.000 description 3
• 230000021615 conjugation Effects 0.000 description 3
• 239000013256 coordination polymer Substances 0.000 description 3
• 230000000593 degrading effect Effects 0.000 description 3
• KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 3
• 238000001514 detection method Methods 0.000 description 3
• 206010013023 diphtheria Diseases 0.000 description 3
• 238000004520 electroporation Methods 0.000 description 3
• 230000002255 enzymatic effect Effects 0.000 description 3
• 238000000605 extraction Methods 0.000 description 3
• 229960000304 folic acid Drugs 0.000 description 3
• 238000010230 functional analysis Methods 0.000 description 3
• 238000001502 gel electrophoresis Methods 0.000 description 3
• 239000008103 glucose Substances 0.000 description 3
• 229960002989 glutamic acid Drugs 0.000 description 3
• KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 3
• RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 3
• 230000036541 health Effects 0.000 description 3
• 230000001771 impaired effect Effects 0.000 description 3
• 230000002779 inactivation Effects 0.000 description 3
• 230000003834 intracellular effect Effects 0.000 description 3
• 238000002093 isoelectric focusing polyacrylamide gel electrophoresis Methods 0.000 description 3
• 238000002955 isolation Methods 0.000 description 3
• 101150087199 leuA gene Proteins 0.000 description 3
• 101150035025 lysC gene Proteins 0.000 description 3
• 239000012092 media component Substances 0.000 description 3
• 108010046778 molybdenum cofactor Proteins 0.000 description 3
• HPEUEJRPDGMIMY-IFQPEPLCSA-N molybdopterin Chemical compound O([C@H]1N2)[C@H](COP(O)(O)=O)C(S)=C(S)[C@@H]1NC1=C2N=C(N)NC1=O HPEUEJRPDGMIMY-IFQPEPLCSA-N 0.000 description 3
• LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 3
• 235000013923 monosodium glutamate Nutrition 0.000 description 3
• 229950006238 nadide Drugs 0.000 description 3
• 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
• BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
• 235000015097 nutrients Nutrition 0.000 description 3
• 230000036961 partial effect Effects 0.000 description 3
• 235000021317 phosphate Nutrition 0.000 description 3
• 230000004147 pyrimidine metabolism Effects 0.000 description 3
• 150000003230 pyrimidines Chemical class 0.000 description 3
• 230000006798 recombination Effects 0.000 description 3
• 238000006722 reduction reaction Methods 0.000 description 3
• 108091008146 restriction endonucleases Proteins 0.000 description 3
• 101150057107 sigB gene Proteins 0.000 description 3
• 239000011734 sodium Substances 0.000 description 3
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
• 239000000243 solution Substances 0.000 description 3
• 239000005720 sucrose Substances 0.000 description 3
• 239000012134 supernatant fraction Substances 0.000 description 3
• 239000000725 suspension Substances 0.000 description 3
• 235000019157 thiamine Nutrition 0.000 description 3
• 229960003495 thiamine Drugs 0.000 description 3
• 239000011721 thiamine Substances 0.000 description 3
• 229960004072 thrombin Drugs 0.000 description 3
• 230000002103 transcriptional effect Effects 0.000 description 3
• 238000001890 transfection Methods 0.000 description 3
• 230000001131 transforming effect Effects 0.000 description 3
• 241000701447 unidentified baculovirus Species 0.000 description 3
• DJJCXFVJDGTHFX-ZAKLUEHWSA-N uridine-5'-monophosphate Chemical compound O[C@@H]1[C@@H](O)[C@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-ZAKLUEHWSA-N 0.000 description 3
• GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 2
• RBCOYOYDYNXAFA-UHFFFAOYSA-L (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl phosphate Chemical compound CC1=NC=C(COP([O-])([O-])=O)C(C)=C1O RBCOYOYDYNXAFA-UHFFFAOYSA-L 0.000 description 2
• RFLVMTUMFYRZCB-UHFFFAOYSA-N 1-methylguanine Chemical compound O=C1N(C)C(N)=NC2=C1N=CN2 RFLVMTUMFYRZCB-UHFFFAOYSA-N 0.000 description 2
• OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
• YSAJFXWTVFGPAX-UHFFFAOYSA-N 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetic acid Chemical compound OC(=O)COC1=CNC(=O)NC1=O YSAJFXWTVFGPAX-UHFFFAOYSA-N 0.000 description 2
• OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
• OIVLITBTBDPEFK-UHFFFAOYSA-N 5,6-dihydrouracil Chemical compound O=C1CCNC(=O)N1 OIVLITBTBDPEFK-UHFFFAOYSA-N 0.000 description 2
• ZLAQATDNGLKIEV-UHFFFAOYSA-N 5-methyl-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound CC1=CNC(=S)NC1=O ZLAQATDNGLKIEV-UHFFFAOYSA-N 0.000 description 2
• HUNCSWANZMJLPM-UHFFFAOYSA-N 5-methyltryptophan Chemical compound CC1=CC=C2NC=C(CC(N)C(O)=O)C2=C1 HUNCSWANZMJLPM-UHFFFAOYSA-N 0.000 description 2
• LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
• ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 2
• 229920001817 Agar Polymers 0.000 description 2
• 102000034263 Amino acid transporters Human genes 0.000 description 2
• 108050005273 Amino acid transporters Proteins 0.000 description 2
• 101710149879 Arginine repressor Proteins 0.000 description 2
• 108010014885 Arginine-tRNA ligase Proteins 0.000 description 2
• 108010055400 Aspartate kinase Proteins 0.000 description 2
• 241000193830 Bacillus <bacterium> Species 0.000 description 2
• 101100096227 Bacteroides fragilis (strain 638R) argF' gene Proteins 0.000 description 2
• 102000007132 Carboxyl and Carbamoyl Transferases Human genes 0.000 description 2
• 108010072957 Carboxyl and Carbamoyl Transferases Proteins 0.000 description 2
• 102000014914 Carrier Proteins Human genes 0.000 description 2
• GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 2
• 108020004705 Codon Proteins 0.000 description 2
• 241000918600 Corynebacterium ulcerans Species 0.000 description 2
• 241000195493 Cryptophyta Species 0.000 description 2
• PCDQPRRSZKQHHS-UHFFFAOYSA-N Cytidine 5'-triphosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-UHFFFAOYSA-N 0.000 description 2
• WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
• SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 description 2
• 238000001712 DNA sequencing Methods 0.000 description 2
• 101710096438 DNA-binding protein Proteins 0.000 description 2
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
• 101710088194 Dehydrogenase Proteins 0.000 description 2
• 108030003594 Diaminopimelate decarboxylases Proteins 0.000 description 2
• 101100129336 Dictyostelium discoideum malA gene Proteins 0.000 description 2
• 108010014468 Dihydrodipicolinate Reductase Proteins 0.000 description 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
• WQXNXVUDBPYKBA-UHFFFAOYSA-N Ectoine Natural products CC1=NCCC(C(O)=O)N1 WQXNXVUDBPYKBA-UHFFFAOYSA-N 0.000 description 2
• 101150034814 F gene Proteins 0.000 description 2
• 241000192125 Firmicutes Species 0.000 description 2
• 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 2
• 108010064711 Homoserine dehydrogenase Proteins 0.000 description 2
• 101150005343 INHA gene Proteins 0.000 description 2
• XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
• 108020003285 Isocitrate lyase Proteins 0.000 description 2
• FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 2
• 229930195722 L-methionine Natural products 0.000 description 2
• GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
• 101100354186 Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) ptcA gene Proteins 0.000 description 2
• HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 description 2
• DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
• 239000000020 Nitrocellulose Substances 0.000 description 2
• 101710163270 Nuclease Proteins 0.000 description 2
• 102000008153 Peptide Elongation Factor Tu Human genes 0.000 description 2
• 108010049977 Peptide Elongation Factor Tu Proteins 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 108700023175 Phosphate acetyltransferases Proteins 0.000 description 2
• 108091000080 Phosphotransferase Proteins 0.000 description 2
• 102100036134 Probable arginine-tRNA ligase, mitochondrial Human genes 0.000 description 2
• 102000001253 Protein Kinase Human genes 0.000 description 2
• 108010076504 Protein Sorting Signals Proteins 0.000 description 2
• 108010026552 Proteome Proteins 0.000 description 2
• 101100217185 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aruC gene Proteins 0.000 description 2
• 108020004511 Recombinant DNA Proteins 0.000 description 2
• 241000316848 Rhodococcus <scale insect> Species 0.000 description 2
• 102000006382 Ribonucleases Human genes 0.000 description 2
• 108010083644 Ribonucleases Proteins 0.000 description 2
• PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
• 101100190460 Shigella flexneri pic gene Proteins 0.000 description 2
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
• 108091081024 Start codon Proteins 0.000 description 2
• 101100022072 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) lysJ gene Proteins 0.000 description 2
• 108090000190 Thrombin Proteins 0.000 description 2
• 102000008579 Transposases Human genes 0.000 description 2
• 108010020764 Transposases Proteins 0.000 description 2
• 102100021436 UDP-glucose 4-epimerase Human genes 0.000 description 2
• 108010075202 UDP-glucose 4-epimerase Proteins 0.000 description 2
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
• 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
• 150000007513 acids Chemical class 0.000 description 2
• 239000008272 agar Substances 0.000 description 2
• HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
• 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
• 230000003321 amplification Effects 0.000 description 2
• 239000005557 antagonist Substances 0.000 description 2
• 239000000427 antigen Substances 0.000 description 2
• 102000036639 antigens Human genes 0.000 description 2
• 239000002246 antineoplastic agent Substances 0.000 description 2
• 239000003963 antioxidant agent Substances 0.000 description 2
• 235000006708 antioxidants Nutrition 0.000 description 2
• 238000013459 approach Methods 0.000 description 2
• YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
• 101150008194 argB gene Proteins 0.000 description 2
• 101150070427 argC gene Proteins 0.000 description 2
• 101150089042 argC2 gene Proteins 0.000 description 2
• 101150050866 argD gene Proteins 0.000 description 2
• 101150056313 argF gene Proteins 0.000 description 2
• 101150029940 argJ gene Proteins 0.000 description 2
• 101150040872 aroE gene Proteins 0.000 description 2
• 101150010999 aroP gene Proteins 0.000 description 2
• 101150107204 asd gene Proteins 0.000 description 2
• 244000052616 bacterial pathogen Species 0.000 description 2
• 230000009286 beneficial effect Effects 0.000 description 2
• UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 2
• GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
• 238000012742 biochemical analysis Methods 0.000 description 2
• 230000004071 biological effect Effects 0.000 description 2
• 101150098189 brnQ gene Proteins 0.000 description 2
• 102000028406 carbon-sulfur lyase Human genes 0.000 description 2
• 108010076637 carbon-sulfur lyase Proteins 0.000 description 2
• 239000000969 carrier Substances 0.000 description 2
• YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
• 230000032823 cell division Effects 0.000 description 2
• 230000003833 cell viability Effects 0.000 description 2
• 239000007795 chemical reaction product Substances 0.000 description 2
• 239000004927 clay Substances 0.000 description 2
• 238000003776 cleavage reaction Methods 0.000 description 2
• 238000002742 combinatorial mutagenesis Methods 0.000 description 2
• 238000010835 comparative analysis Methods 0.000 description 2
• 230000000052 comparative effect Effects 0.000 description 2
• 239000003184 complementary RNA Substances 0.000 description 2
• 238000009833 condensation Methods 0.000 description 2
• 230000005494 condensation Effects 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• PCDQPRRSZKQHHS-ZAKLUEHWSA-N cytidine-5'-triphosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO[P@](O)(=O)O[P@@](O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-ZAKLUEHWSA-N 0.000 description 2
• 229940104302 cytosine Drugs 0.000 description 2
• 230000006378 damage Effects 0.000 description 2
• 101150073654 dapB gene Proteins 0.000 description 2
• 230000003247 decreasing effect Effects 0.000 description 2
• 230000002950 deficient Effects 0.000 description 2
• 235000014113 dietary fatty acids Nutrition 0.000 description 2
• 239000000975 dye Substances 0.000 description 2
• WQXNXVUDBPYKBA-YFKPBYRVSA-N ectoine Chemical compound CC1=[NH+][C@H](C([O-])=O)CCN1 WQXNXVUDBPYKBA-YFKPBYRVSA-N 0.000 description 2
• 210000002889 endothelial cell Anatomy 0.000 description 2
• 230000007613 environmental effect Effects 0.000 description 2
• 239000000284 extract Substances 0.000 description 2
• 229930195729 fatty acid Natural products 0.000 description 2
• 239000000194 fatty acid Substances 0.000 description 2
• 150000004665 fatty acids Chemical class 0.000 description 2
• 239000007850 fluorescent dye Substances 0.000 description 2
• 229940014144 folate Drugs 0.000 description 2
• 230000005714 functional activity Effects 0.000 description 2
• 230000002538 fungal effect Effects 0.000 description 2
• 101150002054 galE gene Proteins 0.000 description 2
• 102000034356 gene-regulatory proteins Human genes 0.000 description 2
• 108091006104 gene-regulatory proteins Proteins 0.000 description 2
• 238000012252 genetic analysis Methods 0.000 description 2
• 235000013922 glutamic acid Nutrition 0.000 description 2
• 239000004220 glutamic acid Substances 0.000 description 2
• 108010064177 glutamine synthetase I Proteins 0.000 description 2
• 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
• 230000034659 glycolysis Effects 0.000 description 2
• 230000002414 glycolytic effect Effects 0.000 description 2
• 239000003102 growth factor Substances 0.000 description 2
• 210000002216 heart Anatomy 0.000 description 2
• 101150086151 hrdB gene Proteins 0.000 description 2
• FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
• 101150118781 icd gene Proteins 0.000 description 2
• 238000001727 in vivo Methods 0.000 description 2
• 238000011065 in-situ storage Methods 0.000 description 2
• 239000000411 inducer Substances 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 229930027917 kanamycin Natural products 0.000 description 2
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
• 229960000318 kanamycin Drugs 0.000 description 2
• 229930182823 kanamycin A Natural products 0.000 description 2
• 238000011005 laboratory method Methods 0.000 description 2
• JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
• 239000007788 liquid Substances 0.000 description 2
• KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
• 210000004185 liver Anatomy 0.000 description 2
• 238000000464 low-speed centrifugation Methods 0.000 description 2
• 101150094164 lysY gene Proteins 0.000 description 2
• 101150039489 lysZ gene Proteins 0.000 description 2
• 235000013372 meat Nutrition 0.000 description 2
• GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 2
• 230000037345 metabolism of vitamins Effects 0.000 description 2
• 235000013379 molasses Nutrition 0.000 description 2
• 229920001220 nitrocellulos Polymers 0.000 description 2
• 108091027963 non-coding RNA Proteins 0.000 description 2
• 102000042567 non-coding RNA Human genes 0.000 description 2
• 238000003199 nucleic acid amplification method Methods 0.000 description 2
• WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
• 238000002515 oligonucleotide synthesis Methods 0.000 description 2
• 230000003287 optical effect Effects 0.000 description 2
• 150000002894 organic compounds Chemical class 0.000 description 2
• 230000003204 osmotic effect Effects 0.000 description 2
• 230000003647 oxidation Effects 0.000 description 2
• 238000007254 oxidation reaction Methods 0.000 description 2
• 101150076071 panD gene Proteins 0.000 description 2
• 229940055726 pantothenic acid Drugs 0.000 description 2
• 244000052769 pathogen Species 0.000 description 2
• 230000001717 pathogenic effect Effects 0.000 description 2
• 230000007170 pathology Effects 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
• 102000020233 phosphotransferase Human genes 0.000 description 2
• 238000013081 phylogenetic analysis Methods 0.000 description 2
• 230000035479 physiological effects, processes and functions Effects 0.000 description 2
• 102000054765 polymorphisms of proteins Human genes 0.000 description 2
• 101150059999 pro gene Proteins 0.000 description 2
• 238000012545 processing Methods 0.000 description 2
• 229940076376 protein agonist Drugs 0.000 description 2
• 229940076372 protein antagonist Drugs 0.000 description 2
• 230000006337 proteolytic cleavage Effects 0.000 description 2
• 210000001938 protoplast Anatomy 0.000 description 2
• 239000002213 purine nucleotide Substances 0.000 description 2
• NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 2
• 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 2
• 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 2
• NHZMQXZHNVQTQA-UHFFFAOYSA-N pyridoxamine Chemical compound CC1=NC=C(CO)C(CN)=C1O NHZMQXZHNVQTQA-UHFFFAOYSA-N 0.000 description 2
• 235000008160 pyridoxine Nutrition 0.000 description 2
• 239000011677 pyridoxine Substances 0.000 description 2
• ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 2
• 229960004172 pyridoxine hydrochloride Drugs 0.000 description 2
• 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 2
• 239000011764 pyridoxine hydrochloride Substances 0.000 description 2
• 108700022487 rRNA Genes Proteins 0.000 description 2
• 150000003254 radicals Chemical class 0.000 description 2
• 230000002285 radioactive effect Effects 0.000 description 2
• 230000009467 reduction Effects 0.000 description 2
• 230000003362 replicative effect Effects 0.000 description 2
• 238000003571 reporter gene assay Methods 0.000 description 2
• 101150102864 rpoD gene Proteins 0.000 description 2
• 230000007017 scission Effects 0.000 description 2
• 239000013049 sediment Substances 0.000 description 2
• 238000007086 side reaction Methods 0.000 description 2
• 101150117326 sigA gene Proteins 0.000 description 2
• 150000003384 small molecules Chemical class 0.000 description 2
• 238000004611 spectroscopical analysis Methods 0.000 description 2
• 238000010186 staining Methods 0.000 description 2
• 239000006228 supernatant Substances 0.000 description 2
• 230000001629 suppression Effects 0.000 description 2
• 230000009885 systemic effect Effects 0.000 description 2
• 238000012360 testing method Methods 0.000 description 2
• 238000004809 thin layer chromatography Methods 0.000 description 2
• 101150000850 thrC gene Proteins 0.000 description 2
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
• 239000011573 trace mineral Substances 0.000 description 2
• 235000013619 trace mineral Nutrition 0.000 description 2
• 230000004102 tricarboxylic acid cycle Effects 0.000 description 2
• 229960004799 tryptophan Drugs 0.000 description 2
• 230000035899 viability Effects 0.000 description 2
• 239000013603 viral vector Substances 0.000 description 2
• 229940011671 vitamin b6 Drugs 0.000 description 2
• 239000011701 zinc Substances 0.000 description 2
• SERHXTVXHNVDKA-UHFFFAOYSA-N (+)-(R)-2,3,4,5-tetrahydro-3-hydroxy-4,4-dimethylfuran-2-one Natural products CC1(C)COC(=O)C1O SERHXTVXHNVDKA-UHFFFAOYSA-N 0.000 description 1
• KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
• HOZBSSWDEKVXNO-BXRBKJIMSA-N (2s)-2-azanylbutanedioic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O.OC(=O)[C@@H](N)CC(O)=O HOZBSSWDEKVXNO-BXRBKJIMSA-N 0.000 description 1
• IAYJZWFYUSNIPN-KFRZSCGFSA-N (2s,3r,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](OC=2C=CC(=CC=2)[N+]([O-])=O)[C@H](O)[C@H]1O IAYJZWFYUSNIPN-KFRZSCGFSA-N 0.000 description 1
• MSTNYGQPCMXVAQ-RYUDHWBXSA-N (6S)-5,6,7,8-tetrahydrofolic acid Chemical compound C([C@H]1CNC=2N=C(NC(=O)C=2N1)N)NC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-RYUDHWBXSA-N 0.000 description 1
• SERHXTVXHNVDKA-BYPYZUCNSA-N (R)-pantolactone Chemical compound CC1(C)COC(=O)[C@@H]1O SERHXTVXHNVDKA-BYPYZUCNSA-N 0.000 description 1
• 229940115459 (r)- pantolactone Drugs 0.000 description 1
• WJNGQIYEQLPJMN-IOSLPCCCSA-N 1-methylinosine Chemical compound C1=NC=2C(=O)N(C)C=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O WJNGQIYEQLPJMN-IOSLPCCCSA-N 0.000 description 1
• VBNGJYNPUFWTKX-UHFFFAOYSA-N 10,10-diamino-1,6-dioxacyclotridecane-2,5,7,13-tetrone Chemical compound NC1(CCC(=O)OC(CCC(=O)OC(CC1)=O)=O)N VBNGJYNPUFWTKX-UHFFFAOYSA-N 0.000 description 1
• 108020004465 16S ribosomal RNA Proteins 0.000 description 1
• UWOCFOFVIBZJGH-UHFFFAOYSA-N 2,3-dihydrodipicolinic acid Chemical compound OC(=O)C1CC=CC(C(O)=O)=N1 UWOCFOFVIBZJGH-UHFFFAOYSA-N 0.000 description 1
• HLYBTPMYFWWNJN-UHFFFAOYSA-N 2-(2,4-dioxo-1h-pyrimidin-5-yl)-2-hydroxyacetic acid Chemical compound OC(=O)C(O)C1=CNC(=O)NC1=O HLYBTPMYFWWNJN-UHFFFAOYSA-N 0.000 description 1
• JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• UDOGNMDURIJYQC-UHFFFAOYSA-N 2-amino-6-methyl-1h-pteridin-4-one Chemical compound N1C(N)=NC(=O)C2=NC(C)=CN=C21 UDOGNMDURIJYQC-UHFFFAOYSA-N 0.000 description 1
• XMSMHKMPBNTBOD-UHFFFAOYSA-N 2-dimethylamino-6-hydroxypurine Chemical compound N1C(N(C)C)=NC(=O)C2=C1N=CN2 XMSMHKMPBNTBOD-UHFFFAOYSA-N 0.000 description 1
• 108010082310 2-hydroxyacid dehydrogenase Proteins 0.000 description 1
• SMADWRYCYBUIKH-UHFFFAOYSA-N 2-methyl-7h-purin-6-amine Chemical compound CC1=NC(N)=C2NC=NC2=N1 SMADWRYCYBUIKH-UHFFFAOYSA-N 0.000 description 1
• AQSRRZGQRFFFGS-UHFFFAOYSA-N 2-methylpyridin-3-ol Chemical compound CC1=NC=CC=C1O AQSRRZGQRFFFGS-UHFFFAOYSA-N 0.000 description 1
• KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
• MNIQECRMTVGZBM-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;7h-purin-6-amine Chemical compound NC1=NC=NC2=C1NC=N2.CN1CCCC1C1=CC=CN=C1 MNIQECRMTVGZBM-UHFFFAOYSA-N 0.000 description 1
• 108010080376 3-Deoxy-7-Phosphoheptulonate Synthase Proteins 0.000 description 1
• KPULXFNPTWGJQH-UHFFFAOYSA-N 3-hydroxy-4-oxo-4-propan-2-yloxybutanoic acid Chemical compound CC(C)OC(=O)C(O)CC(O)=O KPULXFNPTWGJQH-UHFFFAOYSA-N 0.000 description 1
• KOLPWZCZXAMXKS-UHFFFAOYSA-N 3-methylcytosine Chemical compound CN1C(N)=CC=NC1=O KOLPWZCZXAMXKS-UHFFFAOYSA-N 0.000 description 1
• GJAKJCICANKRFD-UHFFFAOYSA-N 4-acetyl-4-amino-1,3-dihydropyrimidin-2-one Chemical compound CC(=O)C1(N)NC(=O)NC=C1 GJAKJCICANKRFD-UHFFFAOYSA-N 0.000 description 1
• OVONXEQGWXGFJD-UHFFFAOYSA-N 4-sulfanylidene-1h-pyrimidin-2-one Chemical compound SC=1C=CNC(=O)N=1 OVONXEQGWXGFJD-UHFFFAOYSA-N 0.000 description 1
• MQJSSLBGAQJNER-UHFFFAOYSA-N 5-(methylaminomethyl)-1h-pyrimidine-2,4-dione Chemical compound CNCC1=CNC(=O)NC1=O MQJSSLBGAQJNER-UHFFFAOYSA-N 0.000 description 1
• PQGCEDQWHSBAJP-TXICZTDVSA-N 5-O-phosphono-alpha-D-ribofuranosyl diphosphate Chemical compound O[C@H]1[C@@H](O)[C@@H](O[P@](O)(=O)OP(O)(O)=O)O[C@@H]1COP(O)(O)=O PQGCEDQWHSBAJP-TXICZTDVSA-N 0.000 description 1
• WPYRHVXCOQLYLY-UHFFFAOYSA-N 5-[(methoxyamino)methyl]-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound CONCC1=CNC(=S)NC1=O WPYRHVXCOQLYLY-UHFFFAOYSA-N 0.000 description 1
• LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
• ZFTBZKVVGZNMJR-UHFFFAOYSA-N 5-chlorouracil Chemical compound ClC1=CNC(=O)NC1=O ZFTBZKVVGZNMJR-UHFFFAOYSA-N 0.000 description 1
• LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 1
• KSNXJLQDQOIRIP-UHFFFAOYSA-N 5-iodouracil Chemical compound IC1=CNC(=O)NC1=O KSNXJLQDQOIRIP-UHFFFAOYSA-N 0.000 description 1
• KELXHQACBIUYSE-UHFFFAOYSA-N 5-methoxy-1h-pyrimidine-2,4-dione Chemical compound COC1=CNC(=O)NC1=O KELXHQACBIUYSE-UHFFFAOYSA-N 0.000 description 1
• DCPSTSVLRXOYGS-UHFFFAOYSA-N 6-amino-1h-pyrimidine-2-thione Chemical compound NC1=CC=NC(S)=N1 DCPSTSVLRXOYGS-UHFFFAOYSA-N 0.000 description 1
• 102100031126 6-phosphogluconolactonase Human genes 0.000 description 1
• 108010029731 6-phosphogluconolactonase Proteins 0.000 description 1
• MSSXOMSJDRHRMC-UHFFFAOYSA-N 9H-purine-2,6-diamine Chemical compound NC1=NC(N)=C2NC=NC2=N1 MSSXOMSJDRHRMC-UHFFFAOYSA-N 0.000 description 1
• 239000007991 ACES buffer Substances 0.000 description 1
• 102000021527 ATP binding proteins Human genes 0.000 description 1
• 108091011108 ATP binding proteins Proteins 0.000 description 1
• 108010058756 ATP phosphoribosyltransferase Proteins 0.000 description 1
• 108091006112 ATPases Proteins 0.000 description 1
• RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
• 108010092060 Acetate kinase Proteins 0.000 description 1
• ORILYTVJVMAKLC-UHFFFAOYSA-N Adamantane Natural products C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 1
• 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 1
• 102100029457 Adenine phosphoribosyltransferase Human genes 0.000 description 1
• 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
• 229920000936 Agarose Polymers 0.000 description 1
• 241000589158 Agrobacterium Species 0.000 description 1
• 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
• 102100027211 Albumin Human genes 0.000 description 1
• 108010088751 Albumins Proteins 0.000 description 1
• 101100163490 Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) aroA1 gene Proteins 0.000 description 1
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
• GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
• 102100023635 Alpha-fetoprotein Human genes 0.000 description 1
• QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
• 108020005544 Antisense RNA Proteins 0.000 description 1
• 241000219195 Arabidopsis thaliana Species 0.000 description 1
• 241000227150 Arthrobacter aureus Species 0.000 description 1
• 244000063299 Bacillus subtilis Species 0.000 description 1
• 235000014469 Bacillus subtilis Nutrition 0.000 description 1
• 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 1
• 101100443932 Bacillus subtilis (strain 168) dppE gene Proteins 0.000 description 1
• 101100082080 Bacillus subtilis (strain 168) pabA gene Proteins 0.000 description 1
• 101100350224 Bacillus subtilis (strain 168) pdhB gene Proteins 0.000 description 1
• 102100026189 Beta-galactosidase Human genes 0.000 description 1
• 239000002028 Biomass Substances 0.000 description 1
• 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical class [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
• UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
• 240000001432 Calendula officinalis Species 0.000 description 1
• 235000005881 Calendula officinalis Nutrition 0.000 description 1
• 108090000489 Carboxy-Lyases Proteins 0.000 description 1
• 108010001857 Cell Surface Receptors Proteins 0.000 description 1
• 102000000844 Cell Surface Receptors Human genes 0.000 description 1
• 101100163308 Clostridium perfringens (strain 13 / Type A) argR1 gene Proteins 0.000 description 1
• 108020004394 Complementary RNA Proteins 0.000 description 1
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
• 102100031725 Cortactin-binding protein 2 Human genes 0.000 description 1
• 101100236536 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) glcB gene Proteins 0.000 description 1
• 108700000035 Corynebacterium glutamicum BetP Proteins 0.000 description 1
• 101900213311 Corynebacterium glutamicum Threonine synthase Proteins 0.000 description 1
• 241001670112 Corynephage 304L Species 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• 241000701022 Cytomegalovirus Species 0.000 description 1
• NGHMDNPXVRFFGS-IUYQGCFVSA-N D-erythrose 4-phosphate Chemical compound O=C[C@H](O)[C@H](O)COP(O)(O)=O NGHMDNPXVRFFGS-IUYQGCFVSA-N 0.000 description 1
• 229930182818 D-methionine Natural products 0.000 description 1
• FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 description 1
• 239000011703 D-panthenol Substances 0.000 description 1
• HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
• KTVPXOYAKDPRHY-SOOFDHNKSA-N D-ribofuranose 5-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O KTVPXOYAKDPRHY-SOOFDHNKSA-N 0.000 description 1
• ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 1
• ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 1
• 230000033616 DNA repair Effects 0.000 description 1
• 230000008265 DNA repair mechanism Effects 0.000 description 1
• 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
• 241000702421 Dependoparvovirus Species 0.000 description 1
• FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
• 101710092096 Diaminopimelate decarboxylase Proteins 0.000 description 1
• 101100465553 Dictyostelium discoideum psmB6 gene Proteins 0.000 description 1
• RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
• 101710196859 Dipeptide-binding protein Proteins 0.000 description 1
• 102000016607 Diphtheria Toxin Human genes 0.000 description 1
• 108010053187 Diphtheria Toxin Proteins 0.000 description 1
• 206010059866 Drug resistance Diseases 0.000 description 1
• 239000004097 EU approved flavor enhancer Substances 0.000 description 1
• 101710083262 Ectin Proteins 0.000 description 1
• 108091006149 Electron carriers Proteins 0.000 description 1
• 108010067770 Endopeptidase K Proteins 0.000 description 1
• 108010013369 Enteropeptidase Proteins 0.000 description 1
• 102100029727 Enteropeptidase Human genes 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• 108010074860 Factor Xa Proteins 0.000 description 1
• GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
• 229930091371 Fructose Natural products 0.000 description 1
• RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
• 239000005715 Fructose Substances 0.000 description 1
• 206010071602 Genetic polymorphism Diseases 0.000 description 1
• 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 1
• 108010052375 Glutamate Dehydrogenase (NADP+) Proteins 0.000 description 1
• 108030000852 Glutamate N-acetyltransferases Proteins 0.000 description 1
• 102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
• 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
• 102000002667 Glycine hydroxymethyltransferase Human genes 0.000 description 1
• 108010043428 Glycine hydroxymethyltransferase Proteins 0.000 description 1
• 201000005569 Gout Diseases 0.000 description 1
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
• 241000288105 Grus Species 0.000 description 1
• 239000007995 HEPES buffer Substances 0.000 description 1
• 241000606768 Haemophilus influenzae Species 0.000 description 1
• 101000713575 Homo sapiens Tubulin beta-3 chain Proteins 0.000 description 1
• 241000701109 Human adenovirus 2 Species 0.000 description 1
• UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical class C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
• 108060003951 Immunoglobulin Proteins 0.000 description 1
• 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
• UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
• 229930010555 Inosine Natural products 0.000 description 1
• 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 1
• 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 1
• LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
• PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
• AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
• 150000008575 L-amino acids Chemical class 0.000 description 1
• FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 description 1
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
• 229930182821 L-proline Natural products 0.000 description 1
• GHSJKUNUIHUPDF-BYPYZUCNSA-N L-thialysine Chemical compound NCCSC[C@H](N)C(O)=O GHSJKUNUIHUPDF-BYPYZUCNSA-N 0.000 description 1
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
• 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
• 102000004317 Lyases Human genes 0.000 description 1
• 108090000856 Lyases Proteins 0.000 description 1
• 239000007993 MOPS buffer Substances 0.000 description 1
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 1
• FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical class [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
• 108090000301 Membrane transport proteins Proteins 0.000 description 1
• 102000003939 Membrane transport proteins Human genes 0.000 description 1
• 101100242684 Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) panD1 gene Proteins 0.000 description 1
• BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
• 108060004795 Methyltransferase Proteins 0.000 description 1
• 102000016397 Methyltransferase Human genes 0.000 description 1
• ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical class [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
• 102000014842 Multidrug resistance proteins Human genes 0.000 description 1
• 108050005144 Multidrug resistance proteins Proteins 0.000 description 1
• 102000016943 Muramidase Human genes 0.000 description 1
• 108010014251 Muramidase Proteins 0.000 description 1
• 101100518501 Mus musculus Spp1 gene Proteins 0.000 description 1
• SGSSKEDGVONRGC-UHFFFAOYSA-N N(2)-methylguanine Chemical compound O=C1NC(NC)=NC2=C1N=CN2 SGSSKEDGVONRGC-UHFFFAOYSA-N 0.000 description 1
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
• FCVIHFVSXHOPSW-YFKPBYRVSA-N N-acetyl-L-gamma-glutamyl phosphate Chemical compound CC(=O)N[C@H](C(O)=O)CCC(=O)OP(O)(O)=O FCVIHFVSXHOPSW-YFKPBYRVSA-N 0.000 description 1
• RFMMMVDNIPUKGG-YFKPBYRVSA-N N-acetyl-L-glutamic acid Chemical compound CC(=O)N[C@H](C(O)=O)CCC(O)=O RFMMMVDNIPUKGG-YFKPBYRVSA-N 0.000 description 1
• 239000006057 Non-nutritive feed additive Substances 0.000 description 1
• 238000000636 Northern blotting Methods 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 108700026244 Open Reading Frames Proteins 0.000 description 1
• AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
• UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
• 102000004132 Ornithine aminotransferases Human genes 0.000 description 1
• 108090000691 Ornithine aminotransferases Proteins 0.000 description 1
• 101710160107 Outer membrane protein A Proteins 0.000 description 1
• 102000004316 Oxidoreductases Human genes 0.000 description 1
• 108090000854 Oxidoreductases Proteins 0.000 description 1
• 102100034574 P protein Human genes 0.000 description 1
• 101710181008 P protein Proteins 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
• 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
• ZNXZGRMVNNHPCA-UHFFFAOYSA-N Pantetheine Natural products OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS ZNXZGRMVNNHPCA-UHFFFAOYSA-N 0.000 description 1
• 241000364057 Peoria Species 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 102000035195 Peptidases Human genes 0.000 description 1
• 108010068204 Peptide Elongation Factors Proteins 0.000 description 1
• 102000002508 Peptide Elongation Factors Human genes 0.000 description 1
• 108010069013 Phenylalanine Hydroxylase Proteins 0.000 description 1
• 102100038223 Phenylalanine-4-hydroxylase Human genes 0.000 description 1
• 102100036162 Phosphatidylinositol N-acetylglucosaminyltransferase subunit H Human genes 0.000 description 1
• 101710109736 Phosphatidylinositol N-acetylglucosaminyltransferase subunit H Proteins 0.000 description 1
• 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 description 1
• 101710177166 Phosphoprotein Proteins 0.000 description 1
• 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
• 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
• ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical class [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
• 101710197985 Probable protein Rev Proteins 0.000 description 1
• 108010078762 Protein Precursors Proteins 0.000 description 1
• 102000014961 Protein Precursors Human genes 0.000 description 1
• 101100406344 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceF gene Proteins 0.000 description 1
• 101100297447 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnB gene Proteins 0.000 description 1
• 206010037660 Pyrexia Diseases 0.000 description 1
• 101100169519 Pyrococcus abyssi (strain GE5 / Orsay) dapAL gene Proteins 0.000 description 1
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
• 108010053763 Pyruvate Carboxylase Proteins 0.000 description 1
• 108010090051 Pyruvate Dehydrogenase Complex Proteins 0.000 description 1
• 102000012751 Pyruvate Dehydrogenase Complex Human genes 0.000 description 1
• 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 1
• 230000006819 RNA synthesis Effects 0.000 description 1
• MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
• 108020001027 Ribosomal DNA Proteins 0.000 description 1
• MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 1
• GBFLZEXEOZUWRN-VKHMYHEASA-M S-carboxylatomethyl-L-cysteine(1-) Chemical compound [O-]C(=O)[C@@H]([NH3+])CSCC([O-])=O GBFLZEXEOZUWRN-VKHMYHEASA-M 0.000 description 1
• 229910003798 SPO2 Inorganic materials 0.000 description 1
• 101100434411 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH1 gene Proteins 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 101100478210 Schizosaccharomyces pombe (strain 972 / ATCC 24843) spo2 gene Proteins 0.000 description 1
• 108091081021 Sense strand Proteins 0.000 description 1
• 101800002927 Small subunit Proteins 0.000 description 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
• 238000002105 Southern blotting Methods 0.000 description 1
• 235000019764 Soybean Meal Nutrition 0.000 description 1
• 108010073771 Soybean Proteins Proteins 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 241000187747 Streptomyces Species 0.000 description 1
• 101100038645 Streptomyces griseus rppA gene Proteins 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• 108700005078 Synthetic Genes Proteins 0.000 description 1
• 108091008874 T cell receptors Proteins 0.000 description 1
• 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
• FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
• 241000223892 Tetrahymena Species 0.000 description 1
• FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical group C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• 108010006873 Threonine Dehydratase Proteins 0.000 description 1
• 108010022394 Threonine synthase Proteins 0.000 description 1
• 102000006843 Threonine synthase Human genes 0.000 description 1
• 108700019146 Transgenes Proteins 0.000 description 1
• 101100187081 Trichormus variabilis (strain ATCC 29413 / PCC 7937) nifS1 gene Proteins 0.000 description 1
• 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
• 108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
• 102100036790 Tubulin beta-3 chain Human genes 0.000 description 1
• MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
• 108090000848 Ubiquitin Proteins 0.000 description 1
• 102000044159 Ubiquitin Human genes 0.000 description 1
• ZVNYJIZDIRKMBF-UHFFFAOYSA-N Vesnarinone Chemical compound C1=C(OC)C(OC)=CC=C1C(=O)N1CCN(C=2C=C3CCC(=O)NC3=CC=2)CC1 ZVNYJIZDIRKMBF-UHFFFAOYSA-N 0.000 description 1
• 108020000999 Viral RNA Proteins 0.000 description 1
• 208000036142 Viral infection Diseases 0.000 description 1
• 241000700605 Viruses Species 0.000 description 1
• 239000005862 Whey Substances 0.000 description 1
• 102000007544 Whey Proteins Human genes 0.000 description 1
• 108010046377 Whey Proteins Proteins 0.000 description 1
• 208000027418 Wounds and injury Diseases 0.000 description 1
• 102100029089 Xylulose kinase Human genes 0.000 description 1
• 240000008042 Zea mays Species 0.000 description 1
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
• INAPMGSXUVUWAF-GCVPSNMTSA-N [(2r,3s,5r,6r)-2,3,4,5,6-pentahydroxycyclohexyl] dihydrogen phosphate Chemical compound OC1[C@H](O)[C@@H](O)C(OP(O)(O)=O)[C@H](O)[C@@H]1O INAPMGSXUVUWAF-GCVPSNMTSA-N 0.000 description 1
• 238000009825 accumulation Methods 0.000 description 1
• 101150036393 aceB gene Proteins 0.000 description 1
• 229960004308 acetylcysteine Drugs 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000003213 activating effect Effects 0.000 description 1
• 230000004913 activation Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000008186 active pharmaceutical agent Substances 0.000 description 1
• 239000013543 active substance Substances 0.000 description 1
• 230000001154 acute effect Effects 0.000 description 1
• 108010064926 acyl-CoA carboxylase Proteins 0.000 description 1
• 101150102866 adc1 gene Proteins 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 230000000996 additive effect Effects 0.000 description 1
• 229960001570 ademetionine Drugs 0.000 description 1
• 210000004100 adrenal gland Anatomy 0.000 description 1
• 238000001042 affinity chromatography Methods 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 239000000556 agonist Substances 0.000 description 1
• 150000001298 alcohols Chemical class 0.000 description 1
• WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
• 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
• OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 1
• 230000004075 alteration Effects 0.000 description 1
• 150000003863 ammonium salts Chemical class 0.000 description 1
• 238000012435 analytical chromatography Methods 0.000 description 1
• 239000003674 animal food additive Substances 0.000 description 1
• 230000001028 anti-proliverative effect Effects 0.000 description 1
• 108091007433 antigens Proteins 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 229940114079 arachidonic acid Drugs 0.000 description 1
• 235000021342 arachidonic acid Nutrition 0.000 description 1
• 101150118463 argG gene Proteins 0.000 description 1
• 101150089004 argR gene Proteins 0.000 description 1
• 101150037081 aroA gene Proteins 0.000 description 1
• 101150090235 aroB gene Proteins 0.000 description 1
• 101150102858 aroD gene Proteins 0.000 description 1
• 101150108612 aroQ gene Proteins 0.000 description 1
• 150000004982 aromatic amines Chemical class 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• 239000012298 atmosphere Substances 0.000 description 1
• 230000002238 attenuated effect Effects 0.000 description 1
• LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 1
• 108010005774 beta-Galactosidase Proteins 0.000 description 1
• 229940000635 beta-alanine Drugs 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 238000007068 beta-elimination reaction Methods 0.000 description 1
• 235000013361 beverage Nutrition 0.000 description 1
• 239000007621 bhi medium Substances 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 230000000975 bioactive effect Effects 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 238000010364 biochemical engineering Methods 0.000 description 1
• 230000003851 biochemical process Effects 0.000 description 1
• 238000005842 biochemical reaction Methods 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 239000012620 biological material Substances 0.000 description 1
• 230000001486 biosynthesis of amino acids Effects 0.000 description 1
• KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
• CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
• 239000011575 calcium Substances 0.000 description 1
• 229910052791 calcium Inorganic materials 0.000 description 1
• 239000001110 calcium chloride Substances 0.000 description 1
• 229910001628 calcium chloride Inorganic materials 0.000 description 1
• 239000001506 calcium phosphate Substances 0.000 description 1
• 229910000389 calcium phosphate Inorganic materials 0.000 description 1
• 235000011010 calcium phosphates Nutrition 0.000 description 1
• 230000000711 cancerogenic effect Effects 0.000 description 1
• 150000001721 carbon Chemical group 0.000 description 1
• 231100000315 carcinogenic Toxicity 0.000 description 1
• 239000003054 catalyst Substances 0.000 description 1
• 230000003197 catalytic effect Effects 0.000 description 1
• 238000000423 cell based assay Methods 0.000 description 1
• 230000005779 cell damage Effects 0.000 description 1
• 230000003915 cell function Effects 0.000 description 1
• 208000037887 cell injury Diseases 0.000 description 1
• 210000002421 cell wall Anatomy 0.000 description 1
• 108091092328 cellular RNA Proteins 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 235000013339 cereals Nutrition 0.000 description 1
• WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
• 229960005091 chloramphenicol Drugs 0.000 description 1
• 150000001805 chlorine compounds Chemical class 0.000 description 1
• ZYWFEOZQIUMEGL-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol;phenol Chemical compound ClC(Cl)Cl.CC(C)CCO.OC1=CC=CC=C1 ZYWFEOZQIUMEGL-UHFFFAOYSA-N 0.000 description 1
• 238000011210 chromatographic step Methods 0.000 description 1
• 238000004587 chromatography analysis Methods 0.000 description 1
• 238000000975 co-precipitation Methods 0.000 description 1
• 229940010007 cobalamins Drugs 0.000 description 1
• 150000001867 cobalamins Chemical class 0.000 description 1
• 239000010941 cobalt Chemical class 0.000 description 1
• 229910017052 cobalt Inorganic materials 0.000 description 1
• GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical class [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
• 238000004040 coloring Methods 0.000 description 1
• 239000008139 complexing agent Substances 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 230000001276 controlling effect Effects 0.000 description 1
• NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
• 229910052802 copper Inorganic materials 0.000 description 1
• 239000010949 copper Substances 0.000 description 1
• 235000005822 corn Nutrition 0.000 description 1
• 239000002285 corn oil Substances 0.000 description 1
• 235000005687 corn oil Nutrition 0.000 description 1
• 238000012937 correction Methods 0.000 description 1
• 230000002596 correlated effect Effects 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 230000009089 cytolysis Effects 0.000 description 1
• 230000001086 cytosolic effect Effects 0.000 description 1
• 229940127089 cytotoxic agent Drugs 0.000 description 1
• 101150011371 dapA gene Proteins 0.000 description 1
• 101150064923 dapD gene Proteins 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 231100000517 death Toxicity 0.000 description 1
• 230000005786 degenerative changes Effects 0.000 description 1
• 239000007857 degradation product Substances 0.000 description 1
• 238000002405 diagnostic procedure Methods 0.000 description 1
• 235000015872 dietary supplement Nutrition 0.000 description 1
• 239000001177 diphosphate Substances 0.000 description 1
• 235000011180 diphosphates Nutrition 0.000 description 1
• 231100000676 disease causative agent Toxicity 0.000 description 1
• 101150027005 divIB gene Proteins 0.000 description 1
• 101150036185 dnaQ gene Proteins 0.000 description 1
• 238000011143 downstream manufacturing Methods 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 239000003814 drug Substances 0.000 description 1
• 101150026384 dtxR gene Proteins 0.000 description 1
• 230000037149 energy metabolism Effects 0.000 description 1
• 238000001952 enzyme assay Methods 0.000 description 1
• 238000001976 enzyme digestion Methods 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 108010056732 factor EF-P Proteins 0.000 description 1
• 230000008713 feedback mechanism Effects 0.000 description 1
• 238000001914 filtration Methods 0.000 description 1
• 239000012847 fine chemical Substances 0.000 description 1
• 239000000796 flavoring agent Substances 0.000 description 1
• 229960002949 fluorouracil Drugs 0.000 description 1
• 150000002224 folic acids Chemical class 0.000 description 1
• 235000013355 food flavoring agent Nutrition 0.000 description 1
• 235000019264 food flavour enhancer Nutrition 0.000 description 1
• 235000003599 food sweetener Nutrition 0.000 description 1
• 235000013611 frozen food Nutrition 0.000 description 1
• 101150002100 ftsK gene Proteins 0.000 description 1
• 101150026421 ftsQ gene Proteins 0.000 description 1
• 101150111615 ftsZ gene Proteins 0.000 description 1
• 239000000417 fungicide Substances 0.000 description 1
• 229930182830 galactose Natural products 0.000 description 1
• 239000007789 gas Substances 0.000 description 1
• 101150019455 gdh gene Proteins 0.000 description 1
• 108091008053 gene clusters Proteins 0.000 description 1
• 238000012224 gene deletion Methods 0.000 description 1
• 238000012239 gene modification Methods 0.000 description 1
• 238000003208 gene overexpression Methods 0.000 description 1
• 230000005017 genetic modification Effects 0.000 description 1
• 235000013617 genetically modified food Nutrition 0.000 description 1
• 101150081163 glnA gene Proteins 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
• 229960002449 glycine Drugs 0.000 description 1
• 239000005090 green fluorescent protein Substances 0.000 description 1
• 239000007952 growth promoter Substances 0.000 description 1
• ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
• 150000003278 haem Chemical class 0.000 description 1
• 229940047650 haemophilus influenzae Drugs 0.000 description 1
• 239000004009 herbicide Substances 0.000 description 1
• 101150063051 hom gene Proteins 0.000 description 1
• 108010034653 homoserine O-acetyltransferase Proteins 0.000 description 1
• 229920002674 hyaluronan Polymers 0.000 description 1
• 229960003160 hyaluronic acid Drugs 0.000 description 1
• 229930195733 hydrocarbon Natural products 0.000 description 1
• 150000002430 hydrocarbons Chemical class 0.000 description 1
• 239000001257 hydrogen Substances 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• 230000007062 hydrolysis Effects 0.000 description 1
• 238000006460 hydrolysis reaction Methods 0.000 description 1
• 101150064958 ideR gene Proteins 0.000 description 1
• 238000003384 imaging method Methods 0.000 description 1
• 102000018358 immunoglobulin Human genes 0.000 description 1
• 229940072221 immunoglobulins Drugs 0.000 description 1
• 229960003444 immunosuppressant agent Drugs 0.000 description 1
• 239000003018 immunosuppressive agent Substances 0.000 description 1
• 239000012535 impurity Substances 0.000 description 1
• 229940097275 indigo Drugs 0.000 description 1
• COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
• 238000009776 industrial production Methods 0.000 description 1
• 208000015181 infectious disease Diseases 0.000 description 1
• 238000001802 infusion Methods 0.000 description 1
• 239000003112 inhibitor Substances 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 208000014674 injury Diseases 0.000 description 1
• 238000011081 inoculation Methods 0.000 description 1
• 229960003786 inosine Drugs 0.000 description 1
• CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
• 239000002917 insecticide Substances 0.000 description 1
• 238000011835 investigation Methods 0.000 description 1
• 150000002500 ions Chemical class 0.000 description 1
• 229960005431 ipriflavone Drugs 0.000 description 1
• 229910052742 iron Inorganic materials 0.000 description 1
• 101150021879 iscS gene Proteins 0.000 description 1
• 108010029918 isocitrate dehydrogenase (NADP+) Proteins 0.000 description 1
• BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
• 210000003734 kidney Anatomy 0.000 description 1
• 239000004310 lactic acid Substances 0.000 description 1
• 235000014655 lactic acid Nutrition 0.000 description 1
• 239000008101 lactose Substances 0.000 description 1
• 238000011031 large-scale manufacturing process Methods 0.000 description 1
• 101150025049 leuB gene Proteins 0.000 description 1
• WQVJUBFKFCDYDQ-BBWFWOEESA-N leubethanol Natural products C1=C(C)C=C2[C@H]([C@H](CCC=C(C)C)C)CC[C@@H](C)C2=C1O WQVJUBFKFCDYDQ-BBWFWOEESA-N 0.000 description 1
• 239000003446 ligand Substances 0.000 description 1
• AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 1
• 238000001638 lipofection Methods 0.000 description 1
• 235000019136 lipoic acid Nutrition 0.000 description 1
• 101150076679 lysG gene Proteins 0.000 description 1
• 229960003646 lysine Drugs 0.000 description 1
• 229960000274 lysozyme Drugs 0.000 description 1
• 239000004325 lysozyme Substances 0.000 description 1
• 235000010335 lysozyme Nutrition 0.000 description 1
• 239000011777 magnesium Chemical class 0.000 description 1
• 229910052749 magnesium Inorganic materials 0.000 description 1
• 210000005075 mammary gland Anatomy 0.000 description 1
• WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical class [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
• 238000004949 mass spectrometry Methods 0.000 description 1
• 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 238000006241 metabolic reaction Methods 0.000 description 1
• 230000037346 metabolism of cofactors Effects 0.000 description 1
• 239000002207 metabolite Substances 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• 229910021645 metal ion Inorganic materials 0.000 description 1
• 229960000485 methotrexate Drugs 0.000 description 1
• IZAGSTRIDUNNOY-UHFFFAOYSA-N methyl 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetate Chemical compound COC(=O)COC1=CNC(=O)NC1=O IZAGSTRIDUNNOY-UHFFFAOYSA-N 0.000 description 1
• LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
• 238000002493 microarray Methods 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 230000002906 microbiologic effect Effects 0.000 description 1
• 238000013048 microbiological method Methods 0.000 description 1
• 235000013336 milk Nutrition 0.000 description 1
• 239000008267 milk Substances 0.000 description 1
• 210000004080 milk Anatomy 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 239000003068 molecular probe Substances 0.000 description 1
• 229910052750 molybdenum Inorganic materials 0.000 description 1
• 239000011733 molybdenum Chemical class 0.000 description 1
• 239000004223 monosodium glutamate Substances 0.000 description 1
• 210000003205 muscle Anatomy 0.000 description 1
• 239000003471 mutagenic agent Substances 0.000 description 1
• XJVXMWNLQRTRGH-UHFFFAOYSA-N n-(3-methylbut-3-enyl)-2-methylsulfanyl-7h-purin-6-amine Chemical compound CSC1=NC(NCCC(C)=C)=C2NC=NC2=N1 XJVXMWNLQRTRGH-UHFFFAOYSA-N 0.000 description 1
• 229930014626 natural product Natural products 0.000 description 1
• 238000001320 near-infrared absorption spectroscopy Methods 0.000 description 1
• 230000001338 necrotic effect Effects 0.000 description 1
• 210000002569 neuron Anatomy 0.000 description 1
• 239000002547 new drug Substances 0.000 description 1
• 229960003966 nicotinamide Drugs 0.000 description 1
• 235000005152 nicotinamide Nutrition 0.000 description 1
• 239000011570 nicotinamide Substances 0.000 description 1
• 101150082753 nifS gene Proteins 0.000 description 1
• 150000002823 nitrates Chemical class 0.000 description 1
• 229910017464 nitrogen compound Inorganic materials 0.000 description 1
• 150000002830 nitrogen compounds Chemical class 0.000 description 1
• QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
• 230000005257 nucleotidylation Effects 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 238000005457 optimization Methods 0.000 description 1
• 230000008520 organization Effects 0.000 description 1
• 229960003104 ornithine Drugs 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 230000002018 overexpression Effects 0.000 description 1
• 238000012261 overproduction Methods 0.000 description 1
• KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
• JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
• LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 1
• 210000000496 pancreas Anatomy 0.000 description 1
• ZNXZGRMVNNHPCA-VIFPVBQESA-N pantetheine Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS ZNXZGRMVNNHPCA-VIFPVBQESA-N 0.000 description 1
• 230000007918 pathogenicity Effects 0.000 description 1
• 238000010647 peptide synthesis reaction Methods 0.000 description 1
• 210000000578 peripheral nerve Anatomy 0.000 description 1
• 238000002823 phage display Methods 0.000 description 1
• 239000012071 phase Substances 0.000 description 1
• 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
• DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 1
• 108010085336 phosphoribosyl-AMP cyclohydrolase Proteins 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 230000026731 phosphorylation Effects 0.000 description 1
• 238000006366 phosphorylation reaction Methods 0.000 description 1
• LYCRXMTYUZDUGA-UYRKPTJQSA-N pimeloyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCCCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LYCRXMTYUZDUGA-UYRKPTJQSA-N 0.000 description 1
• 229920002401 polyacrylamide Polymers 0.000 description 1
• 230000008488 polyadenylation Effects 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1
• 229920001296 polysiloxane Polymers 0.000 description 1
• 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• 150000004032 porphyrins Chemical class 0.000 description 1
• 230000004481 post-translational protein modification Effects 0.000 description 1
• 239000011591 potassium Chemical class 0.000 description 1
• 229910052700 potassium Inorganic materials 0.000 description 1
• 239000008057 potassium phosphate buffer Substances 0.000 description 1
• 230000003389 potentiating effect Effects 0.000 description 1
• 244000144977 poultry Species 0.000 description 1
• 235000013324 preserved food Nutrition 0.000 description 1
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
• 238000011027 product recovery Methods 0.000 description 1
• 108010066823 proline dipeptidase Proteins 0.000 description 1
• ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
• 235000019833 protease Nutrition 0.000 description 1
• 125000006239 protecting group Chemical group 0.000 description 1
• 230000004853 protein function Effects 0.000 description 1
• 108060006633 protein kinase Proteins 0.000 description 1
• 230000009145 protein modification Effects 0.000 description 1
• 238000001742 protein purification Methods 0.000 description 1
• 239000012264 purified product Substances 0.000 description 1
• 101150096049 pyc gene Proteins 0.000 description 1
• OYSBZLVHMPNJMR-UHFFFAOYSA-N pyridine-3-carboxylic acid Chemical compound OC(=O)C1=CC=CN=C1.OC(=O)C1=CC=CN=C1 OYSBZLVHMPNJMR-UHFFFAOYSA-N 0.000 description 1
• 150000003222 pyridines Chemical class 0.000 description 1
• 235000008151 pyridoxamine Nutrition 0.000 description 1
• 239000011699 pyridoxamine Substances 0.000 description 1
• WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical class OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 1
• 238000011002 quantification Methods 0.000 description 1
• 230000005855 radiation Effects 0.000 description 1
• MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
• 108020003175 receptors Proteins 0.000 description 1
• 102000005962 receptors Human genes 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 238000006268 reductive amination reaction Methods 0.000 description 1
• 238000007670 refining Methods 0.000 description 1
• 230000014493 regulation of gene expression Effects 0.000 description 1
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
• 230000009711 regulatory function Effects 0.000 description 1
• 210000002345 respiratory system Anatomy 0.000 description 1
• 238000010839 reverse transcription Methods 0.000 description 1
• 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
• 239000010979 ruby Substances 0.000 description 1
• 229910001750 ruby Inorganic materials 0.000 description 1
• 238000007423 screening assay Methods 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 238000002864 sequence alignment Methods 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 229910052709 silver Inorganic materials 0.000 description 1
• 239000004332 silver Substances 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 229910052708 sodium Inorganic materials 0.000 description 1
• 239000001632 sodium acetate Substances 0.000 description 1
• 235000017281 sodium acetate Nutrition 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
• 229940073490 sodium glutamate Drugs 0.000 description 1
• 239000007787 solid Substances 0.000 description 1
• 239000007790 solid phase Substances 0.000 description 1
• 238000000527 sonication Methods 0.000 description 1
• 229940001941 soy protein Drugs 0.000 description 1
• 239000004455 soybean meal Substances 0.000 description 1
• 210000000952 spleen Anatomy 0.000 description 1
• 238000003153 stable transfection Methods 0.000 description 1
• 238000007447 staining method Methods 0.000 description 1
• 238000012409 standard PCR amplification Methods 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 238000011146 sterile filtration Methods 0.000 description 1
• 238000012916 structural analysis Methods 0.000 description 1
• 235000000346 sugar Nutrition 0.000 description 1
• 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
• 230000004083 survival effect Effects 0.000 description 1
• 239000003765 sweetening agent Substances 0.000 description 1
• 238000001308 synthesis method Methods 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• 235000002906 tartaric acid Nutrition 0.000 description 1
• 239000011975 tartaric acid Substances 0.000 description 1
• 150000003505 terpenes Chemical class 0.000 description 1
• 238000010998 test method Methods 0.000 description 1
• 239000005460 tetrahydrofolate Substances 0.000 description 1
• 229960002663 thioctic acid Drugs 0.000 description 1
• ZEMGGZBWXRYJHK-UHFFFAOYSA-N thiouracil Chemical compound O=C1C=CNC(=S)N1 ZEMGGZBWXRYJHK-UHFFFAOYSA-N 0.000 description 1
• 101150014006 thrA gene Proteins 0.000 description 1
• 101150072448 thrB gene Proteins 0.000 description 1
• 210000001685 thyroid gland Anatomy 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• 238000005891 transamination reaction Methods 0.000 description 1
• 238000010361 transduction Methods 0.000 description 1
• 230000026683 transduction Effects 0.000 description 1
• 238000003151 transfection method Methods 0.000 description 1
• 238000011426 transformation method Methods 0.000 description 1
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
• 125000002264 triphosphate group Chemical group [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
• 101150035767 trp gene Proteins 0.000 description 1
• 101150044170 trpE gene Proteins 0.000 description 1
• 101150034486 trpG gene Proteins 0.000 description 1
• 210000004881 tumor cell Anatomy 0.000 description 1
• 238000000108 ultra-filtration Methods 0.000 description 1
• 241000701161 unidentified adenovirus Species 0.000 description 1
• 241001515965 unidentified phage Species 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 229940035893 uracil Drugs 0.000 description 1
• 230000004143 urea cycle Effects 0.000 description 1
• MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
• FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
• 235000012141 vanillin Nutrition 0.000 description 1
• 230000009385 viral infection Effects 0.000 description 1
• 238000001262 western blot Methods 0.000 description 1
• 229940075420 xanthine Drugs 0.000 description 1
• 108091022915 xylulokinase Proteins 0.000 description 1
• 210000005253 yeast cell Anatomy 0.000 description 1
• 229910052725 zinc Inorganic materials 0.000 description 1