CN1391604A - 用于无蛋白无血清培养细胞的培养基 - Google Patents
用于无蛋白无血清培养细胞的培养基 Download PDFInfo
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Abstract
Description
细胞克隆 | 工作细胞的细胞比产出率(mU rvWF/106细胞/天) | 10个世代后细胞比产出率(mUrvWF/106细胞/天) | 60个世代后细胞比产出率(mU rvWF/106细胞/天) |
rvWF-CHO#808.68原始细胞克隆 | 55 | 30 | <10 |
r-vWF-CHOF7*)稳定的克隆 | 62 | 65 | 60 |
成分 | g/L | 优选的量(按照我们在本专利申请时的知识)(g/L) |
合成的基本培养基(DMEM/HAM’s F12) | 1-100 | 11.00-12.00 |
大豆蛋白胨 | 0.5-50 | 2.5 |
L-谷氨酰胺 | 0.05-1 | 0.36 |
抗坏血酸 | 0.0005-0.05 | 0.0035 |
NaHCO3 | 0.1-10 | 2.00 |
乙醇胺 | 0.0005-0.05 | 0.0015 |
亚硒酸钠 | 1-15μg/l | 8.6μg/l |
任选的:Synperonic F68 | 0.01-10 | 0.25 |
培养的天数 | 细胞比产出率(mU/106细胞/天) | 免疫荧光(%FVIII阳性细胞) |
15 | 702 | n.a. |
21 | 1125 | n.a. |
28 | 951 | >95% |
35 | 691 | >95% |
42 | 970 | n.a. |
氨基酸: | mg/l | 优选的量(按照我们在本专利申请时的知识)(mg/L) |
L-天冬酰胺 | 1-100 | 20 |
L-半胱氨酸·HCl·H2O | 1-100 | 15 |
L-胱氨酸 | 1-100 | 20 |
L-脯氨酸 | 1-150 | 35 |
L-谷氨酰胺 | 50-1000 | 240 |
水解产物 | 终细胞密度(×106/mL) | FVIII效价(mU/mL) | FVIII凝血活性(mU/mL) |
大豆 | 3.6 | 485 | 508 |
酵母 | 3.3 | 226 | 230 |
水解产物 | 终细胞密度(×106/mL) | FVIII效价(mU/mL) | vWF-抗原(μg/mL) |
大豆 | 3.7 | 1142 | 6.7 |
水稻 | 3.0 | 479 | 3.2 |
小麦 | 3.4 | 522 | 3.9 |
对照 | 3.0 | 406 | 3.1 |
水解产物 | 终细胞密度(×106/mL) | FVIII效价(mU/mL) | FVIII-抗原(μg/mL) | VWF-抗原(μg/mL) |
大豆 | 1.6 | 1427 | 166 | 17.2 |
小麦 | 1.0 | 1120 | 92 | 7.9 |
Claims (16)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ATA1659/99 | 1999-09-28 | ||
AT0165999A AT409379B (de) | 1999-06-02 | 1999-09-28 | Medium zur protein- und serumfreien kultivierung von zellen |
ATA1659/1999 | 1999-09-28 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN2007100968436A Division CN101173246B (zh) | 1999-09-28 | 2000-09-27 | 用于无蛋白无血清培养细胞的培养基 |
Publications (2)
Publication Number | Publication Date |
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CN1391604A true CN1391604A (zh) | 2003-01-15 |
CN1318577C CN1318577C (zh) | 2007-05-30 |
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CN2007100968436A Expired - Lifetime CN101173246B (zh) | 1999-09-28 | 2000-09-27 | 用于无蛋白无血清培养细胞的培养基 |
CNB008160201A Expired - Lifetime CN1318577C (zh) | 1999-09-28 | 2000-09-27 | 用于无蛋白无血清培养细胞的培养基 |
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CN2007100968436A Expired - Lifetime CN101173246B (zh) | 1999-09-28 | 2000-09-27 | 用于无蛋白无血清培养细胞的培养基 |
Country Status (21)
Country | Link |
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US (6) | US20030203448A1 (zh) |
EP (1) | EP1220893B2 (zh) |
JP (6) | JP5441288B2 (zh) |
CN (2) | CN101173246B (zh) |
AT (2) | AT409379B (zh) |
AU (1) | AU780791B2 (zh) |
CA (1) | CA2385299A1 (zh) |
CY (1) | CY1106135T1 (zh) |
CZ (1) | CZ305307B6 (zh) |
DE (1) | DE60028989T3 (zh) |
DK (1) | DK1220893T4 (zh) |
ES (1) | ES2265991T5 (zh) |
HU (1) | HU228210B1 (zh) |
IL (4) | IL148642A0 (zh) |
PL (1) | PL215234B1 (zh) |
PT (1) | PT1220893E (zh) |
RU (3) | RU2266325C2 (zh) |
SI (1) | SI1220893T1 (zh) |
SK (1) | SK288059B6 (zh) |
TR (1) | TR200200757T2 (zh) |
WO (1) | WO2001023527A1 (zh) |
Cited By (7)
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CN101603026B (zh) * | 2009-06-19 | 2011-01-19 | 华东理工大学 | 适于动物细胞产品生产的无动物来源低蛋白培养基 |
CN102115728B (zh) * | 2009-12-31 | 2012-09-26 | 北京清大天一科技有限公司 | 无血清动物细胞培养基干粉、液体培养基及其制备方法 |
CN103160458A (zh) * | 2011-12-15 | 2013-06-19 | 西南民族大学 | 一种适合Vero细胞生长的低血清培养基 |
CN104302759A (zh) * | 2012-03-08 | 2015-01-21 | 菲仕兰产品有限公司 | 用于真核细胞的培养基 |
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CN102115728B (zh) * | 2009-12-31 | 2012-09-26 | 北京清大天一科技有限公司 | 无血清动物细胞培养基干粉、液体培养基及其制备方法 |
CN103160458A (zh) * | 2011-12-15 | 2013-06-19 | 西南民族大学 | 一种适合Vero细胞生长的低血清培养基 |
CN104302759A (zh) * | 2012-03-08 | 2015-01-21 | 菲仕兰产品有限公司 | 用于真核细胞的培养基 |
CN104593317A (zh) * | 2013-10-31 | 2015-05-06 | 中国食品发酵工业研究院 | 一种用于细胞培养基的大豆活性肽添加剂 |
CN105002242A (zh) * | 2015-07-23 | 2015-10-28 | 苏州康聚生物科技有限公司 | 用于cho细胞中高效表达重组人促甲状腺激素的无血清培养基及其应用 |
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