AU551324B2 - Phosphoramidite derivatives of nucleotides and their use in preparation of oligonucleotides - Google Patents

Phosphoramidite derivatives of nucleotides and their use in preparation of oligonucleotides

Info

Publication number
AU551324B2
AU551324B2 AU81997/82A AU8199782A AU551324B2 AU 551324 B2 AU551324 B2 AU 551324B2 AU 81997/82 A AU81997/82 A AU 81997/82A AU 8199782 A AU8199782 A AU 8199782A AU 551324 B2 AU551324 B2 AU 551324B2
Authority
AU
Australia
Prior art keywords
oligonucleotides
nucleotides
preparation
phosphoramidite derivatives
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU81997/82A
Other languages
English (en)
Other versions
AU8199782A (en
Inventor
Serge L. Beaucage
Marvin Harry Caruthers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University Patents Inc
Original Assignee
University Patents Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22939185&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU551324(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University Patents Inc filed Critical University Patents Inc
Publication of AU8199782A publication Critical patent/AU8199782A/en
Application granted granted Critical
Publication of AU551324B2 publication Critical patent/AU551324B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Luminescent Compositions (AREA)
AU81997/82A 1981-03-27 1982-03-26 Phosphoramidite derivatives of nucleotides and their use in preparation of oligonucleotides Expired AU551324B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US248450 1981-03-27
US06/248,450 US4415732A (en) 1981-03-27 1981-03-27 Phosphoramidite compounds and processes

Publications (2)

Publication Number Publication Date
AU8199782A AU8199782A (en) 1982-09-30
AU551324B2 true AU551324B2 (en) 1986-04-24

Family

ID=22939185

Family Applications (1)

Application Number Title Priority Date Filing Date
AU81997/82A Expired AU551324B2 (en) 1981-03-27 1982-03-26 Phosphoramidite derivatives of nucleotides and their use in preparation of oligonucleotides

Country Status (8)

Country Link
US (1) US4415732A (cg-RX-API-DMAC7.html)
EP (1) EP0061746B1 (cg-RX-API-DMAC7.html)
JP (2) JPS57176998A (cg-RX-API-DMAC7.html)
AT (1) ATE12236T1 (cg-RX-API-DMAC7.html)
AU (1) AU551324B2 (cg-RX-API-DMAC7.html)
CA (1) CA1203237A (cg-RX-API-DMAC7.html)
DE (1) DE3262598D1 (cg-RX-API-DMAC7.html)
MX (1) MX157501A (cg-RX-API-DMAC7.html)

Families Citing this family (714)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035719B1 (en) * 1980-02-29 1989-09-20 University Patents, Inc. Process for producing modified inorganic polymers, their use in producing polynucleotides, and a reagent useful in these processes
US7101981B1 (en) 1980-08-26 2006-09-05 Regents Of The University Of California Bovine growth hormone recombinantly produced in E. coli
US6692941B1 (en) * 1980-08-26 2004-02-17 The Regents Of The University Of California Bovine growth hormone
US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
JPS58109498A (ja) * 1981-08-24 1983-06-29 Agency Of Ind Science & Technol オリゴリボヌクレオチド
EP0090789A1 (en) * 1982-03-26 1983-10-05 Monsanto Company Chemical DNA synthesis
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
JPS59501830A (ja) * 1982-09-30 1984-11-01 アクテイエボラゲツト　イロ 横糸を蓄積、供給および測定する装置および該装置を制御するための方法
DE3239887A1 (de) * 1982-10-28 1984-05-03 Hubert Prof. Dr. 2000 Hamburg Köster Verfahren zur herstellung von oligonucleotiden
US5198540A (en) * 1982-10-28 1993-03-30 Hubert Koster Process for the preparation of oligonucleotides in solution
FR2544720B1 (fr) * 1983-04-19 1987-11-27 California Inst Of Techn Installation perfectionnee et procede pour la synthese de composes chimiques, en particulier d'oligonucleotides
WO1984004749A1 (en) * 1983-06-01 1984-12-06 Beckman Instruments Inc Novel preparation of nucleoside phosphoramidite intermediates
DE3329892A1 (de) * 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
USRE34069E (en) * 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
US5166330A (en) * 1983-09-06 1992-11-24 Hoechst Aktiengesellschaft Process for the preparation of nucleoside alkyl-aralkyl- and aryl-phosphonites and -phosphonates
DE3332068A1 (de) * 1983-09-06 1985-03-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von nukleosidalkyl-, aralkyl- und arylphosphoniten und -phosphonaten
FR2556726B1 (fr) * 1983-12-20 1987-02-20 California Inst Of Techn Compositions a base d'oligonucleotides monocatenaires et procede pour leur preparation
US5118802A (en) * 1983-12-20 1992-06-02 California Institute Of Technology DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
US5118800A (en) * 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US5015733A (en) * 1983-12-20 1991-05-14 California Institute Of Technology Nucleosides possessing blocked aliphatic amino groups
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
JPS61112093A (ja) * 1984-11-05 1986-05-30 Wakunaga Seiyaku Kk ヌクレオチド誘導体
FR2575751B1 (fr) * 1985-01-08 1987-04-03 Pasteur Institut Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques
DE3507881A1 (de) * 1985-03-06 1986-09-11 Hoechst Ag, 6230 Frankfurt Verfahren und mittel zur phosphorylierung
US5332845A (en) * 1985-03-28 1994-07-26 Chiron Corporation Phosphorylating reagents
US5252760A (en) * 1985-03-28 1993-10-12 Chiron Corporation Method of using colored phosphorylating reagents
JPH0723393B2 (ja) * 1985-05-15 1995-03-15 アモコ・コーポレーション シチジン同族体
US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US4797355A (en) * 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
WO1986007362A1 (en) * 1985-06-14 1986-12-18 University Patents, Inc. Method for synthesizing deoxyoligonucleotides
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
EP0216357A3 (en) * 1985-09-25 1988-08-31 Nippon Zeon Co., Ltd. Phosphoramidite compounds and process for production thereof
US4808708A (en) * 1985-11-02 1989-02-28 Toagosei Chemical Industry Co., Ltd. Phosphorsulfide derivatives of deoxynucleosides or deoxynucleotides and their uses
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US5242823A (en) * 1986-03-07 1993-09-07 International Genetic Engineering, Inc. Cloning of the 38kd Mycoplasma hyorhinis regression-associated antigen
DE3788914T2 (de) * 1986-09-08 1994-08-25 Ajinomoto Kk Verbindungen zur Spaltung von RNS an eine spezifische Position, Oligomere, verwendet bei der Herstellung dieser Verbindungen und Ausgangsprodukte für die Synthese dieser Oligomere.
EP0289619B1 (en) * 1986-10-30 1995-08-16 Daicel Chemical Industries, Ltd. Process for synthesizing oligonucleotides and compounds for forming high-molecular protective group
US5071974A (en) * 1986-10-31 1991-12-10 Amoco Corporation Compositions and methods for the synthesis of oligonucleotides having 5'-phosphorylated termini
US4923901A (en) * 1987-09-04 1990-05-08 Millipore Corporation Membranes with bound oligonucleotides and peptides
US6031091A (en) * 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
US5585481A (en) * 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
WO1989009780A1 (en) * 1988-04-15 1989-10-19 Chemical Industry Institute Of Toxicology Site-specific tritium-labeled oligodeoxynucleotides
US5278302A (en) * 1988-05-26 1994-01-11 University Patents, Inc. Polynucleotide phosphorodithioates
US5750666A (en) * 1988-05-26 1998-05-12 Competitve Technologies, Inc. Polynucleotide phosphorodithioate compounds
US5602244A (en) * 1988-05-26 1997-02-11 Competitive Technologies, Inc. Polynucleotide phosphorodithioate compounds
IL90359A0 (en) * 1988-05-26 1989-12-15 University Patents Inc Nucleoside and polynucleotide thiophosphoramidite and phosphorodithioate compounds and their production
US5218103A (en) * 1988-05-26 1993-06-08 University Patents, Inc. Nucleoside thiophosphoramidites
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0291088A (ja) * 1988-09-29 1990-03-30 Central Glass Co Ltd ホスファイトおよびヌクレオシド‐３’‐ホスファイト誘導体およびこれを用いるオリゴヌクレオチドの合成法
US5262530A (en) * 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
CA2028153A1 (en) * 1989-02-24 1990-08-25 Steven S. Smith Heterologous block oligomers
AU5418290A (en) * 1989-03-31 1990-11-05 University Patents Inc. Polynucleotide phosphorodithioates as therapeutic agents for retroviral infections
US6254861B1 (en) 1989-05-23 2001-07-03 Chandra Choudhury Hematopoietic growth factor derived from T lymphocytes
DE3916871A1 (de) * 1989-05-24 1990-11-29 Boehringer Mannheim Gmbh Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren
GR890100349A (el) * 1989-05-26 1994-03-31 University Patents Inc Ενώσεις νουκλεοσιδίων & πολυνουκλεοτιδίων, θειοφωσφοραμιδίων & φωσφοροδιθειικών εστέρων & μέθοδοι.
US5256775A (en) * 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
EP0476071A4 (en) * 1989-06-05 1992-11-04 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides and methods for preparing the same
US6300486B1 (en) 1989-06-15 2001-10-09 Isis Pharmaceuticals, Inc. Large scale synthesis of oligonucleotides and their associated analogs
US5164491A (en) * 1989-06-15 1992-11-17 Gilead Sciences Large scale synthesis of oligonucleotides and their associated analogs
US5204455A (en) * 1989-06-15 1993-04-20 Froehler Brian C Monomethoxytrityl protected oligonucleotides bound to a solid support
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5003097A (en) * 1989-10-02 1991-03-26 The United States Of America As Represented By The Department Of Health And Human Services Method for the sulfurization of phosphorous groups in compounds
AU6603690A (en) * 1989-10-05 1991-04-28 University Patents Inc. Nucleoside and polynucleotide thiophosphoramidite and phosphorodithioate compounds and processes
US5914396A (en) * 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
EP0463712A3 (en) 1990-06-27 1992-04-08 University Patents, Inc. Polynucleotide phosphorodithioates as therapeutic agents for retroviral infections
US5316908A (en) * 1990-07-13 1994-05-31 Life Technologies, Inc. Size markers for electrophoretic analysis of DNA
EP0592434B1 (en) * 1990-10-26 1998-10-07 Genta Incorporated Improved process for the synthesis of oligomers
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
US5594121A (en) * 1991-11-07 1997-01-14 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US5556961A (en) * 1991-11-15 1996-09-17 Foote; Robert S. Nucleosides with 5'-O-photolabile protecting groups
US8153602B1 (en) 1991-11-19 2012-04-10 Isis Pharmaceuticals, Inc. Composition and methods for the pulmonary delivery of nucleic acids
EP0637965B1 (en) * 1991-11-26 2002-10-16 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US6235887B1 (en) 1991-11-26 2001-05-22 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines
TW393513B (en) * 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
EP0616612A4 (en) 1991-12-12 1995-01-11 Gilead Sciences Inc NUCLEASE-STABLE OLIGOMERS SUITABLE FOR BINDING AND METHODS OF USE.
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5652099A (en) * 1992-02-12 1997-07-29 Conrad; Michael J. Probes comprising fluorescent nucleosides and uses thereof
EP0628051B1 (en) * 1992-02-12 2003-07-02 Chromagen, Inc. Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
US5428148A (en) * 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5616700A (en) * 1992-04-24 1997-04-01 Beckman Instruments, Inc. Processes for synthesizing nucleotides and modified nucleotides using N.sub.
US5348868A (en) * 1992-04-24 1994-09-20 Beckman Instruments, Inc. Methods and reagents for cleaving and deprotecting oligonucleotides
US5583211A (en) * 1992-10-29 1996-12-10 Beckman Instruments, Inc. Surface activated organic polymers useful for location - specific attachment of nucleic acids, peptides, proteins and oligosaccharides
US5955591A (en) * 1993-05-12 1999-09-21 Imbach; Jean-Louis Phosphotriester oligonucleotides, amidites and method of preparation
US5428149A (en) * 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
EP0705335A1 (en) 1993-06-23 1996-04-10 Genesys Pharma Inc. Antisense oligonucleotides and therapeutic use thereof in human immunodeficiency virus infection
US5623068A (en) * 1994-03-07 1997-04-22 Beckman Instruments, Inc. Synthesis of DNA using substituted phenylacetyl-protected nucleotides
US6639061B1 (en) 1999-07-07 2003-10-28 Isis Pharmaceuticals, Inc. C3′-methylene hydrogen phosphonate oligomers and related compounds
US5510476A (en) * 1994-07-07 1996-04-23 Isis Pharmaceuticals, Inc. Carbocation scavenging during oligonucleotide synthesis
US5552471A (en) * 1994-08-17 1996-09-03 The Perkin-Elmer Corporation Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides
US5703223A (en) * 1994-09-02 1997-12-30 Thomas Jefferson University Solid phase synthesis of oligonucleotides with stereospecific substituted phosphonate linkages by pentavalent grignard coupling
US6558633B1 (en) * 1994-09-21 2003-05-06 Isis Pharmaceuticals, Inc. Chemical reaction apparatus and methods
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5717085A (en) * 1994-11-23 1998-02-10 Terrapin Technologies, Inc. Process for preparing codon amidites
US5908926A (en) * 1995-03-16 1999-06-01 Duke University 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5614622A (en) * 1995-06-01 1997-03-25 Hybridon, Inc. 5-pentenoyl moiety as a nucleoside-amino protecting group, 4-pentenoyl-protected nucleotide synthons, and related oligonucleotide syntheses
US6531589B1 (en) * 1995-06-01 2003-03-11 Hybridon, Inc. Base protecting groups and synthons for oligonucleotide synthesis
US6111095A (en) * 1995-06-07 2000-08-29 Merck & Co., Inc. Capped synthetic RNA, analogs, and aptamers
US5856464A (en) * 1995-06-07 1999-01-05 Lajolla Pharmaceutical Company Selective capping solution phase oligonucleotide synthesis
ATE466109T1 (de) 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
US5889136A (en) * 1995-06-09 1999-03-30 The Regents Of The University Of Colorado Orthoester protecting groups in RNA synthesis
AU712779C (en) * 1995-10-19 2002-02-07 Proligo Llc Method for solution phase synthesis of oligonucleotides
US5734041A (en) * 1995-10-20 1998-03-31 Mcgill University Preparation of chiral phosphorothioate oligomers
US6160109A (en) * 1995-10-20 2000-12-12 Isis Pharmaceuticals, Inc. Preparation of phosphorothioate and boranophosphate oligomers
US6476216B1 (en) 1995-10-20 2002-11-05 Mcgill University Preparation of phosphorothioate oligomers
US5705621A (en) * 1995-11-17 1998-01-06 Isis Pharmaceuticals, Inc. Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same
US20030119724A1 (en) * 1995-11-22 2003-06-26 Ts`O Paul O.P. Ligands to enhance cellular uptake of biomolecules
US5808035A (en) * 1995-12-08 1998-09-15 Usher; David A. Protected nucleoside and method for its synthesis
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
CA2250923A1 (en) * 1996-03-26 1997-10-02 Gabriella Zupi Oligonucleotide treatments and compositions for human melanoma
US5959100A (en) 1996-03-27 1999-09-28 Nexstar Pharmaceuticals, Inc. Pyrimidine nucleosides as therapeutic and diagnostic agents
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5852168A (en) * 1996-04-30 1998-12-22 Regents Of The University Of Minesota Sulfurization of phosphorus-containing compounds
US7812149B2 (en) 1996-06-06 2010-10-12 Isis Pharmaceuticals, Inc. 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations
US5898031A (en) 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
US9096636B2 (en) 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US6172217B1 (en) 1996-12-27 2001-01-09 Isis Pharmaceuticals Inc. Method of synthesizing phosphorothioate oligonucleotides
US5955600A (en) * 1996-12-27 1999-09-21 Isis Pharmaceuticals, Inc. Method for the synthesis of nucleotide or oligonucleotide phosphoramidites
US6103891A (en) * 1996-12-27 2000-08-15 Isis Pharmaceuticals, Inc. Method for the synthesis of nucleotide or oligonucleotide phosphoramidites
US5760209A (en) * 1997-03-03 1998-06-02 Isis Pharmaceuticals, Inc. Protecting group for synthesizing oligonucleotide analogs
US5902881A (en) * 1997-03-03 1999-05-11 Isis Pharmaceuticals, Inc. Reagent useful for synthesizing sulfurized oligonucleotide analogs
CA2283789C (en) 1997-03-12 2005-07-05 The Perkin-Elmer Corporation Dna polymerases having improved labeled nucleotide incorporation properties
WO1998044156A1 (en) * 1997-03-27 1998-10-08 Life Technologies, Inc. Nucleic acid ladders
WO1999003872A1 (en) 1997-07-15 1999-01-28 Life Technologies, Inc. Nucleic acid ladders
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6583168B1 (en) 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6441150B1 (en) 1997-12-16 2002-08-27 Isis Pharmaceuticals, Inc. Compounds for the Synthesis of Nucleotide or oligonucleotide phosphoramidites
US7427678B2 (en) * 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
CA2316912C (en) * 1998-01-12 2009-09-15 Massachusetts Institute Of Technology Method and apparatus for performing microassays
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US20030027173A1 (en) * 1998-01-16 2003-02-06 Della-Cioppa Guy Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
US20020164585A1 (en) * 1998-01-16 2002-11-07 Sean Chapman Method for enhancing RNA or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6326479B1 (en) 1998-01-27 2001-12-04 Boston Probes, Inc. Synthetic polymers and methods, kits or compositions for modulating the solubility of same
US6020475A (en) * 1998-02-10 2000-02-01 Isis Pharmeuticals, Inc. Process for the synthesis of oligomeric compounds
US7273933B1 (en) 1998-02-26 2007-09-25 Isis Pharmaceuticals, Inc. Methods for synthesis of oligonucleotides
US6111086A (en) * 1998-02-27 2000-08-29 Scaringe; Stephen A. Orthoester protecting groups
US6531590B1 (en) * 1998-04-24 2003-03-11 Isis Pharmaceuticals, Inc. Processes for the synthesis of oligonucleotide compounds
US6590092B1 (en) * 1998-05-19 2003-07-08 Nam Q. Ngo Process for preparing a “universal support” and the reagents used for generating such support
US6274725B1 (en) 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
US6919437B1 (en) 1998-06-11 2005-07-19 Isis Pharmaceuticals, Inc. Synthetic methods and intermediates for triester oligonucleotides
US6335439B1 (en) 1998-06-11 2002-01-01 Isis Pharmaceuticals, Inc. Method of preparing phosphoramidites
US6270730B1 (en) 1998-06-16 2001-08-07 Northwest Engineering Inc. Multi-well rotary synthesizer
US7087148B1 (en) 1998-06-23 2006-08-08 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
US6326478B1 (en) 1998-07-08 2001-12-04 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
US6242589B1 (en) 1998-07-14 2001-06-05 Isis Pharmaceuticals, Inc. Phosphorothioate oligonucleotides having modified internucleoside linkages
US6867294B1 (en) 1998-07-14 2005-03-15 Isis Pharmaceuticals, Inc. Gapped oligomers having site specific chiral phosphorothioate internucleoside linkages
US6124484A (en) 1998-07-17 2000-09-26 Isis Pharmaceuticals, Inc. Recovery of triarylmethyl halide protecting groups cleaved during oligonucleotide synthesis
US6271042B1 (en) 1998-08-26 2001-08-07 Alpha Innotech Corporation Biochip detection system
US6335437B1 (en) 1998-09-07 2002-01-01 Isis Pharmaceuticals, Inc. Methods for the preparation of conjugated oligomers
US6069243A (en) * 1998-10-06 2000-05-30 Isis Pharmaceuticals, Inc. Process for oligonucleotide synthesis
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6153411A (en) 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US6169177B1 (en) 1998-11-06 2001-01-02 Isis Pharmaceuticals, Inc. Processes for the synthesis of oligomeric compounds
ATE319857T1 (de) 1998-12-14 2006-03-15 Li Cor Inc Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
US6380378B1 (en) 1998-12-24 2002-04-30 Toagosei Company, Ltd. Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
US6207819B1 (en) 1999-02-12 2001-03-27 Isis Pharmaceuticals, Inc. Compounds, processes and intermediates for synthesis of mixed backbone oligomeric compounds
US6121437A (en) * 1999-03-16 2000-09-19 Isis Pharmaceuticals, Inc. Phosphate and thiophosphate protecting groups
US20040082774A1 (en) * 1999-03-16 2004-04-29 Guzaev Andrei P. Novel phosphate and thiophosphate protecting groups
US6399765B1 (en) * 1999-03-17 2002-06-04 Isis Pharmaceuticals, Inc. Methods for removing dimethoxytrityl groups from oligonucleotides
EP1165235B1 (en) 1999-03-19 2011-09-28 Life Technologies Corporation Method of screening mutated cells
EP1163251A1 (en) 1999-03-24 2001-12-19 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES N-acylphosphoramidites and their use in oligonucleotide synthesis
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US6221635B1 (en) 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
US6610842B1 (en) * 1999-05-06 2003-08-26 Isis Pharmaceuticals, Inc. Processes for the synthesis of oligomers using phosphoramidite compositions
JP2003522119A (ja) * 1999-05-24 2003-07-22 インビトロジェン コーポレイション 標識されたオリゴヌクレオチドの脱ブロック化方法
US20070059752A1 (en) * 1999-06-09 2007-03-15 Biosearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
US7183405B2 (en) 1999-06-25 2007-02-27 Syn Gen, Inc. Compositions and methods for labeling oligonucleotides
US6750357B1 (en) 1999-06-25 2004-06-15 Syngen, Inc. Rhodamine-based fluorophores useful as labeling reagents
US6414135B1 (en) 1999-07-07 2002-07-02 Isis Pharmaceuticals, Inc. C3′-methylene hydrogen phosphonate monomers and related compounds
US6593466B1 (en) 1999-07-07 2003-07-15 Isis Pharmaceuticals, Inc. Guanidinium functionalized nucleotides and precursors thereof
US6306599B1 (en) 1999-07-16 2001-10-23 Agilent Technologies Inc. Biopolymer arrays and their fabrication
US6346423B1 (en) 1999-07-16 2002-02-12 Agilent Technologies, Inc. Methods and compositions for producing biopolymeric arrays
US6147200A (en) * 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
US6114518A (en) * 1999-09-30 2000-09-05 Becton, Dickinson And Company Synthesis and use of labelled phosphoramidite compositions
WO2001025486A1 (en) 1999-10-04 2001-04-12 University Of Medicine And Dentistry Of New Jersey Methods for identifying rna binding compounds
US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
CN1402796A (zh) * 1999-11-02 2003-03-12 胡元徽 分子微阵列及其制备方法和用途
US6361958B1 (en) 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
EP1233976A4 (en) * 1999-11-17 2003-06-11 Jiuping Ji SIMULTANEOUS DETECTION OF HBV, HCV AND HIV IN PLASMA SAMPLES WITH THE AID OF A MULTIPLEX FIXING TEST
US6716994B1 (en) * 2000-01-04 2004-04-06 Applera Corporation Mobility-Modifying Cyanine Dyes
US7332271B2 (en) * 2000-02-18 2008-02-19 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
EP1313880A2 (en) * 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
EP1287154A4 (en) 2000-06-07 2010-12-15 Pacific Biosciences California NUCLEOTIDES WITH CHARGE SWITCHING
US7135565B2 (en) * 2000-07-28 2006-11-14 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
ATE432994T1 (de) 2000-09-26 2009-06-15 Boston Probes Inc Sonden, sondensätzen,verfahren und kits zur nachweis, identifizierung und/oder die zählung von bakterien
JP4741170B2 (ja) * 2000-10-11 2011-08-03 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー アニオン性リンカーを有する蛍光核酸塩基結合体
US6693187B1 (en) * 2000-10-17 2004-02-17 Lievre Cornu Llc Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge
WO2002038728A2 (en) * 2000-11-09 2002-05-16 Invitrogen Corporation Method for removing a universal linker from an oligonucleotide
CN100406065C (zh) 2000-12-01 2008-07-30 细胞工厂治疗公司 糖基化/半乳糖基化肽、双官能接头和核苷酸单体/多聚体的缀合物以及相关的组合物和使用方法
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US6825338B2 (en) 2001-03-30 2004-11-30 Isis Pharmaceuticals, Inc. Labeled oligonucleotides, methods for making same, and compounds useful therefor
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
WO2002080849A2 (en) * 2001-04-06 2002-10-17 University Of Chicago Chemotherapeutic induction of egr-1 promoter activity
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
US20050221304A1 (en) * 2001-04-11 2005-10-06 Charlie Xiang Modified random primers for probe labeling
US20030170675A1 (en) * 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
CA2381367A1 (en) 2001-04-12 2002-10-12 Hironori Komatsu Method for purifying 5'-protected 2'-deoxypurine nucleosides
US6867050B2 (en) * 2001-05-16 2005-03-15 Academia Sinica Apparatus and methods for chemical synthesis
US20050009101A1 (en) * 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
JP2005505749A (ja) 2001-05-21 2005-02-24 アクララ バイオサイエンシーズ， インコーポレイテッド タンパク質を分析するための方法および組成物
EP2364990A1 (en) 2001-05-25 2011-09-14 Duke University Modulators of pharmacological agents
EP2305699B1 (de) * 2001-06-05 2014-08-13 CureVac GmbH Stabilisierte mRNA mit erhöhtem G/C-Gehalt und optimierter Codon Usage für die Impfung gegen Schlafkrankheit, Leishmaniose und Toxoplasmose
US7118907B2 (en) * 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
WO2003003063A2 (en) * 2001-06-28 2003-01-09 Ia, Inc. Fiber-optic sensor array
US7153954B2 (en) 2001-07-12 2006-12-26 Santaris Pharma A/S Method for preparation of LNA phosphoramidites
EP1485707B1 (en) * 2001-07-16 2009-01-14 caprotec bioanalytics GmbH Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
US6822088B2 (en) 2001-07-17 2004-11-23 Isis Pharmaceuticals, Inc. Synthesis of oligonucleotides on solid support
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003048179A2 (en) * 2001-12-03 2003-06-12 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
DE10162480A1 (de) 2001-12-19 2003-08-07 Ingmar Hoerr Die Applikation von mRNA für den Einsatz als Therapeutikum gegen Tumorerkrankungen
US7255874B1 (en) 2001-12-21 2007-08-14 Closure Medical Corporation Biocompatible polymers and adhesives: compositions, methods of making and uses related thereto
GB0200526D0 (en) * 2002-01-11 2002-02-27 Science And Technology The Methods
US7098326B2 (en) * 2002-01-23 2006-08-29 Sigma-Aldrich Co. Methods for the integrated synthesis and purification of oligonucleotides
ATE529512T1 (de) 2002-02-01 2011-11-15 Life Technologies Corp Doppelsträngige oligonukleotide
EP1572902B1 (en) 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
DK1502102T3 (da) * 2002-03-11 2009-05-04 Caprotec Bioanalytics Gmbh Forbindelser og fremgangsmåder til analysering af proteomet
CA2477670A1 (en) * 2002-03-15 2003-09-25 Arcturus Bioscience, Inc. Improved nucleic acid amplification
US7169916B2 (en) * 2002-04-01 2007-01-30 Isis Pharmaceuticals, Inc. Chloral-free DCA in oligonucleotide synthesis
JP4583923B2 (ja) 2002-05-08 2010-11-17 パナソニック株式会社 生体分子基板ならびにそれを利用した検査および診断の方法および装置
WO2003102216A2 (en) * 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
CN100424091C (zh) 2002-06-05 2008-10-08 三井化学株式会社 保护化2’-脱氧胞苷类的精制法
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
DE10229872A1 (de) * 2002-07-03 2004-01-29 Curevac Gmbh Immunstimulation durch chemisch modifizierte RNA
US7504215B2 (en) 2002-07-12 2009-03-17 Affymetrix, Inc. Nucleic acid labeling methods
EP1386925A1 (en) * 2002-07-31 2004-02-04 Girindus AG Method for preparing oligonucleotides
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
US20040137469A1 (en) * 2002-09-08 2004-07-15 Casale Ralph A Methods, compositions and libraries pertaining PNA dimer and PNA oligomer synthesis
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US7030230B2 (en) 2002-10-25 2006-04-18 Isis Pharmaceuticals, Inc. Process of purifying phosphoramidites
US9150605B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
WO2004044139A2 (en) 2002-11-05 2004-05-27 Isis Parmaceuticals, Inc. Modified oligonucleotides for use in rna interference
US9150606B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
WO2004052908A1 (ja) * 2002-12-09 2004-06-24 Mitsui Chemicals, Inc. フォスフォロアミダイトの製造方法
GB0229423D0 (en) * 2002-12-18 2003-01-22 Avecia Ltd Process
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
WO2004058794A1 (en) * 2002-12-31 2004-07-15 Proligo Llc Methods and compositions for the tandem synthesis of two or more oligonuleotides on the same solid support
CA2658334C (en) * 2003-01-16 2012-07-10 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
US7002006B2 (en) * 2003-02-12 2006-02-21 Isis Pharmaceuticals, Inc. Protection of nucleosides
JP3936300B2 (ja) * 2003-02-28 2007-06-27 独立行政法人科学技術振興機構 新規なグアノシン誘導体及びその用途
JP2006521116A (ja) 2003-03-27 2006-09-21 ピーティーシー セラピューティクス，インコーポレーテッド 抗真菌性および／または抗増殖性分子を同定するためのｔＲＮＡスプライシング経路の酵素の標的化
US20070020630A1 (en) 2003-03-27 2007-01-25 Trotta Christopher R Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-proliferative agents
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US20070179100A1 (en) * 2003-04-09 2007-08-02 Muthiah Manoharan Protected monomers
US7612197B2 (en) * 2003-05-09 2009-11-03 The United States of America as repesented by the Secretary of the Department of Health and Human Services Thermolabile hydroxyl protecting groups and methods of use
EP1660512A4 (en) 2003-06-02 2009-12-23 Isis Pharmaceuticals Inc SYNTHESIS OF OLIGONUCLEOTIDES USING ALTERNATIVE SOLVENTS
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
DE10335833A1 (de) 2003-08-05 2005-03-03 Curevac Gmbh Transfektion von Blutzellen mit mRNA zur Immunstimulation und Gentherapie
WO2005027962A1 (en) 2003-09-18 2005-03-31 Isis Pharmaceuticals, Inc. 4’-thionucleosides and oligomeric compounds
EP1662863A4 (en) * 2003-09-18 2009-01-28 Mayo Foundation TRANSGENIC NON-TIMES TIEG ANIMALS
DK2708541T3 (en) 2003-11-13 2015-02-23 Isis Pharmaceuticals Inc 5,6-dihydroxy-isoindole derivatives as linkers for oligomeric solid phase synthesis
WO2005062947A2 (en) 2003-12-22 2005-07-14 The Trustees Of The University Of Pennsylvania Methods and compositions for identifying rna-binding proteins
CA2553426A1 (en) * 2004-01-15 2005-08-04 Alza Corporation Liposome composition for delivery of therapeutic agents
US7691316B2 (en) * 2004-02-12 2010-04-06 Chemistry & Technology For Genes, Inc. Devices and methods for the synthesis of nucleic acids
US8569474B2 (en) 2004-03-09 2013-10-29 Isis Pharmaceuticals, Inc. Double stranded constructs comprising one or more short strands hybridized to a longer strand
US8105554B2 (en) 2004-03-12 2012-01-31 Life Technologies Corporation Nanoliter array loading
AU2005238490B2 (en) 2004-04-22 2010-11-18 Tobira Therapeutics, Inc. Improved modulators of coagulation factors
JP4592060B2 (ja) * 2004-04-26 2010-12-01 キヤノン株式会社 Ｐｃｒ増幅反応装置、ならびに、該装置を利用するｐｃｒ増幅反応方法
IL162276A0 (en) * 2004-06-01 2005-11-20 Hadasit Med Res Service Nucleic acid molecules as heparanase potent inhibitors, compositions and methods of use thereof
US8394947B2 (en) 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
US20090048192A1 (en) * 2004-06-03 2009-02-19 Isis Pharmaceuticals, Inc. Double Strand Compositions Comprising Differentially Modified Strands for Use in Gene Modulation
US12070731B2 (en) 2004-08-04 2024-08-27 Life Technologies Corporation Methods and systems for aligning dispensing arrays with microfluidic sample arrays
US20060105453A1 (en) 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
WO2006023880A2 (en) 2004-08-23 2006-03-02 Isis Pharmaceuticals, Inc. Compounds and methods for the characterization of oligonucleotides
DE102004042546A1 (de) * 2004-09-02 2006-03-09 Curevac Gmbh Kombinationstherapie zur Immunstimulation
US7884086B2 (en) 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
SG155923A1 (en) 2004-09-16 2009-10-29 Applied Biosystems Llc Fluorescent dye compounds, conjugates and uses thereof
US20100111856A1 (en) 2004-09-23 2010-05-06 Herman Gill Zirconium-radiolabeled, cysteine engineered antibody conjugates
CN104447992A (zh) 2004-09-23 2015-03-25 健泰科生物技术公司 半胱氨酸改造的抗体和偶联物
WO2006065751A2 (en) * 2004-12-13 2006-06-22 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
US20060142228A1 (en) 2004-12-23 2006-06-29 Ambion, Inc. Methods and compositions concerning siRNA's as mediators of RNA interference
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
US7608433B2 (en) 2005-02-09 2009-10-27 Idexx Laboratories Method of detection of classical swine fever
CA2597673C (en) 2005-02-11 2014-07-08 Harold Varmus Methods and compositions for detecting a drug resistant egfr mutant
DE102005023170A1 (de) 2005-05-19 2006-11-23 Curevac Gmbh Optimierte Formulierung für mRNA
WO2006127009A1 (en) 2005-05-26 2006-11-30 Eragen Biosciences, Inc. Reagent for the improved synthesis of isoguanosine-containing oligonucleotides
US20090270479A1 (en) * 2005-07-12 2009-10-29 Antonio Giordano Genetic and Epigenetic Alterations In the Diagnosis and Treatment of Cancer
US7892795B2 (en) 2005-08-02 2011-02-22 Focus Diagnostics, Inc. Methods and compositions for detecting BK virus
WO2007047408A2 (en) * 2005-10-12 2007-04-26 Pathologica, Llc. Promac signature application
EP1949152A4 (en) * 2005-11-14 2014-02-05 Bayer Ip Gmbh Planar waveguide detection chips and chambers for performing multiplex pcr assays
US7579459B2 (en) * 2005-12-12 2009-08-25 Ngo Nam Q Activator bound solid supports for nucleic acid synthesis via the phosphoramidite approach
US7569686B1 (en) 2006-01-27 2009-08-04 Isis Pharmaceuticals, Inc. Compounds and methods for synthesis of bicyclic nucleic acid analogs
CN102908630B (zh) 2006-01-27 2014-11-19 Isis制药公司 6-修饰的双环核酸类似物
CA3185510A1 (en) 2006-02-13 2007-08-23 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primers and probes for detection and discrimination of types and subtypes of influenza viruses
WO2007126941A2 (en) * 2006-03-24 2007-11-08 Archemix Corp. Solid support reagents for synthesis
US20090292006A1 (en) 2006-05-05 2009-11-26 Sanjay Bhanot Compounds and methods for modulating expression of dgat2
US7666854B2 (en) 2006-05-11 2010-02-23 Isis Pharmaceuticals, Inc. Bis-modified bicyclic nucleic acid analogs
JP5441688B2 (ja) 2006-05-11 2014-03-12 アイシス ファーマシューティカルズ， インコーポレーテッド ５’修飾二環式核酸類似体
WO2007147110A2 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
JP2009543862A (ja) 2006-07-14 2009-12-10 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ガンのバイオマーカーおよびその使用方法
DE102006035618A1 (de) * 2006-07-31 2008-02-07 Curevac Gmbh Nukleinsäure der Formel (I): GlXmGn, insbesondere als immunstimulierendes Adjuvanz
AU2007280690C1 (en) 2006-07-31 2012-08-23 Curevac Gmbh Nucleic acid of formula (I): GIXmGn, or (II): CIXmCn, in particular as an immune-stimulating agent/adjuvant
US8138330B2 (en) * 2006-09-11 2012-03-20 Sigma-Aldrich Co. Llc Process for the synthesis of oligonucleotides
DE102007001370A1 (de) 2007-01-09 2008-07-10 Curevac Gmbh RNA-kodierte Antikörper
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2160396B1 (en) * 2007-05-10 2018-11-21 Agilent Technologies, Inc. Thiocarbon-protecting groups for rna synthesis
US20090131355A1 (en) * 2007-05-23 2009-05-21 Adrian Ion Bot Multicistronic vectors and methods for their design
DK2170917T3 (da) 2007-05-30 2012-10-08 Isis Pharmaceuticals Inc N-Substituerede bicycliske nukleinsyreanaloge med aminomethylenbro
ES2386492T3 (es) 2007-06-08 2012-08-21 Isis Pharmaceuticals, Inc. Análogos de ácidos nucleicos bicíclicos carbocíclicos
WO2008157696A2 (en) 2007-06-19 2008-12-24 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion
ES2376507T5 (es) 2007-07-05 2015-08-31 Isis Pharmaceuticals, Inc. Análogos de ácidos nucleicos bicíclicos 6-disustituidos
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2188298B1 (en) 2007-08-15 2013-09-18 Isis Pharmaceuticals, Inc. Tetrahydropyran nucleic acid analogs
WO2009030254A1 (en) 2007-09-04 2009-03-12 Curevac Gmbh Complexes of rna and cationic peptides for transfection and for immunostimulation
WO2009039173A2 (en) 2007-09-19 2009-03-26 Applied Biosystems Inc. SiRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAi, AND STABILIZED FORMS THEREOF
WO2009046738A1 (en) 2007-10-09 2009-04-16 Curevac Gmbh Composition for treating lung cancer, particularly of non-small lung cancers (nsclc)
EP3222290A1 (en) 2007-10-09 2017-09-27 CureVac AG Composition for treating prostate cancer (pca)
US7829735B2 (en) * 2007-10-26 2010-11-09 Northwestern University Universal phosphoramidite for preparation of modified biomolecules and surfaces
KR20100102107A (ko) * 2007-11-06 2010-09-20 파마이센시아 코퍼레이션 β-뉴클레오시드의 새로운 합성 방법
US9226959B2 (en) 2008-01-31 2016-01-05 Curevac Ag Nucleic acids comprising formula (NuGlXmGnNv)a and derivatives thereof as immunostimulating agent/adjuvant
US8530640B2 (en) 2008-02-07 2013-09-10 Isis Pharmaceuticals, Inc. Bicyclic cyclohexitol nucleic acid analogs
US10131904B2 (en) 2008-02-11 2018-11-20 Rxi Pharmaceuticals Corporation Modified RNAi polynucleotides and uses thereof
WO2009117589A1 (en) 2008-03-21 2009-09-24 Isis Pharmaceuticals, Inc. Oligomeric compounds comprising tricyclic nucleosides and methods for their use
DK2285819T3 (da) 2008-04-04 2013-12-02 Isis Pharmaceuticals Inc Oligomere forbindelser omfattende neutralt bundne, terminale bicykliske nukleosider
NZ597292A (en) 2008-05-14 2013-06-28 Genomic Health Inc Use of PTP4A3 to predict a patient's response to treatment with an EGF receptor inhibitor
WO2010005693A1 (en) * 2008-06-23 2010-01-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cyclized ngr peptide compounds, compositions, and methods of their use
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
US8815818B2 (en) 2008-07-18 2014-08-26 Rxi Pharmaceuticals Corporation Phagocytic cell delivery of RNAI
US8664189B2 (en) 2008-09-22 2014-03-04 Rxi Pharmaceuticals Corporation RNA interference in skin indications
EP2346883B1 (en) 2008-09-23 2016-03-23 Scott G. Petersen Self delivering bio-labile phosphate protected pro-oligos for oligonucleotide based therapeutics and mediating rna interference
WO2010036696A1 (en) 2008-09-24 2010-04-01 Isis Pharmaceuticals, Inc. Cyclohexenyl nucleic acid analogs
EP2356129B1 (en) 2008-09-24 2013-04-03 Isis Pharmaceuticals, Inc. Substituted alpha-l-bicyclic nucleosides
WO2010037408A1 (en) 2008-09-30 2010-04-08 Curevac Gmbh Composition comprising a complexed (m)rna and a naked mrna for providing or enhancing an immunostimulatory response in a mammal and uses thereof
CA2740785C (en) 2008-10-15 2017-06-20 Isis Pharmaceuticals, Inc. Modulation of factor 11 expression
EP2447274B1 (en) 2008-10-24 2017-10-04 Ionis Pharmaceuticals, Inc. Oligomeric compounds and methods
WO2010048552A2 (en) 2008-10-24 2010-04-29 Isis Pharmaceuticals, Inc. Methods of using oligomeric compounds comprising 2'-substituted nucleosides
WO2010059226A2 (en) 2008-11-19 2010-05-27 Rxi Pharmaceuticals Corporation Inhibition of map4k4 through rnai
CN102282155B (zh) 2008-12-02 2017-06-09 日本波涛生命科学公司 磷原子修饰的核酸的合成方法
US9493774B2 (en) 2009-01-05 2016-11-15 Rxi Pharmaceuticals Corporation Inhibition of PCSK9 through RNAi
WO2010090857A2 (en) 2009-01-21 2010-08-12 Vertex Pharmaceuticals Incorporated Methods for amplifying hepatitis c virus nucleic acids
WO2010090762A1 (en) 2009-02-04 2010-08-12 Rxi Pharmaceuticals Corporation Rna duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
WO2010090969A1 (en) 2009-02-06 2010-08-12 Isis Pharmaceuticals, Inc. Tetrahydropyran nucleic acid analogs
US8835117B2 (en) 2009-05-29 2014-09-16 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Nucleic acids for detection and discrimination of genotypes of Chlamydophila psittaci
BR112012000828A8 (pt) 2009-07-06 2017-10-10 Ontorii Inc Novas pró-drogas de ácido nucleico e métodos de uso das mesmas
EP2278334A1 (en) 2009-07-22 2011-01-26 Etablissement Francais du Sang A membrane based microarray device for high-throughput multianalyte detection
US9012421B2 (en) 2009-08-06 2015-04-21 Isis Pharmaceuticals, Inc. Bicyclic cyclohexose nucleic acid analogs
US20110053829A1 (en) 2009-09-03 2011-03-03 Curevac Gmbh Disulfide-linked polyethyleneglycol/peptide conjugates for the transfection of nucleic acids
CN107337734A (zh) 2009-12-04 2017-11-10 弗·哈夫曼-拉罗切有限公司 多特异性抗体、抗体类似物、组合物和方法
WO2011069529A1 (en) 2009-12-09 2011-06-16 Curevac Gmbh Mannose-containing solution for lyophilization, transfection and/or injection of nucleic acids
WO2011069528A1 (en) 2009-12-09 2011-06-16 Curevac Gmbh Lyophilization of nucleic acids in lactate-containing solutions
US8779118B2 (en) 2010-01-11 2014-07-15 Isis Pharmaceuticals, Inc. Base modified bicyclic nucleosides and oligomeric compounds prepared therefrom
WO2011094580A2 (en) 2010-01-28 2011-08-04 Alnylam Pharmaceuticals, Inc. Chelated copper for use in the preparation of conjugated oligonucleotides
US10113206B2 (en) 2010-02-24 2018-10-30 Grifols Therapeutics Inc. Methods, compositions, and kits for determining human immunodeficiency virus (HIV)
WO2011115818A1 (en) 2010-03-17 2011-09-22 Isis Pharmaceuticals, Inc. 5'-substituted bicyclic nucleosides and oligomeric compounds prepared therefrom
US9340786B2 (en) 2010-03-24 2016-05-17 Rxi Pharmaceuticals Corporation RNA interference in dermal and fibrotic indications
CN106074591B (zh) 2010-03-24 2020-01-14 菲奥医药公司 眼部症候中的rna干扰
WO2011119852A1 (en) 2010-03-24 2011-09-29 Rxi Pharmaceuticals Corporation Reduced size self-delivering rnai compounds
EP2558478B1 (en) 2010-04-12 2018-03-07 Somalogic, Inc. 5-position modified pyrimidines and their use
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
WO2011139695A2 (en) 2010-04-28 2011-11-10 Isis Pharmaceuticals, Inc. Modified 5' diphosphate nucleosides and oligomeric compounds prepared therefrom
EP2625186B1 (en) 2010-04-28 2016-07-27 Ionis Pharmaceuticals, Inc. 5' modified nucleosides and oligomeric compounds prepared therefrom
US8993738B2 (en) 2010-04-28 2015-03-31 Isis Pharmaceuticals, Inc. Modified nucleosides, analogs thereof and oligomeric compounds prepared therefrom
WO2011156278A1 (en) 2010-06-07 2011-12-15 Isis Pharmaceuticals, Inc. Bicyclic nucleosides and oligomeric compounds prepared therefrom
US8846637B2 (en) 2010-06-08 2014-09-30 Isis Pharmaceuticals, Inc. Substituted 2′-amino and 2′-thio-bicyclic nucleosides and oligomeric compounds prepared therefrom
KR101839163B1 (ko) 2010-06-08 2018-03-15 제넨테크, 인크. 시스테인 조작된 항체 및 접합체
CN107648604A (zh) 2010-07-30 2018-02-02 库瑞瓦格股份公司 聚合物载体运载物复合物及聚合物载体的用途
EP2600901B1 (en) 2010-08-06 2019-03-27 ModernaTX, Inc. A pharmaceutical formulation comprising engineered nucleic acids and medical use thereof
EP2620428B1 (en) 2010-09-24 2019-05-22 Wave Life Sciences Ltd. Asymmetric auxiliary group
MX2013003681A (es) 2010-10-01 2013-11-20 Moderna Therapeutics Inc Ácidos nucleicos manipulados y métodos de uso de los mismos.
CA3077910A1 (en) 2010-11-17 2012-05-24 Ionis Pharmaceuticals, Inc. Modulation of alpha synuclein expression
AR084358A1 (es) 2010-12-27 2013-05-08 Lilly Co Eli Composiciones y metodos para identificar y diferenciar componentes virales de vacunas multivalentes de la “fiebre de embarque” (complejo de enfermedad respiratoria bovina (brdc))
ES2709190T3 (es) 2010-12-27 2019-04-15 Elanco Us Inc Composiciones de vacuna contra el virus de la diarrea viral bovina tipo 1B y procedimientos
CN103403163B (zh) 2010-12-30 2016-10-26 陶氏益农公司 靶向液泡atp酶c亚基并赋予对鞘翅目有害生物的抗性的核酸分子
RU2013135491A (ru) 2010-12-30 2015-02-10 Дау Агросайенсиз Ллс Молекулы нуклеиновых кислот, которые нацелены на малый gtp-связывающий белок rho1 и сообщают устойчивость к вредителям отряда жесткокрылых
AU2011352005B2 (en) 2010-12-30 2017-03-16 Dow Agrosciences Llc Nucleic acid molecules that confer resistance to coleopteran pests
UY33853A (es) 2010-12-30 2012-07-31 Dow Agrosciences Llc ?moléculas de ácido nucleico que se dirigen a la subunidad h de la atpasa vacuolar y confieren resistencia a plagas de coleópteros?.
WO2012135805A2 (en) 2011-03-31 2012-10-04 modeRNA Therapeutics Delivery and formulation of engineered nucleic acids
EP2801626B1 (en) 2011-05-24 2016-04-06 ELITechGroup B.V. Detection of methicillin-resistant staphylococcus aureus
WO2012170347A1 (en) 2011-06-09 2012-12-13 Isis Pharmaceuticals, Inc. Bicyclic nucleosides and oligomeric compounds prepared therefrom
UY34128A (es) 2011-06-15 2013-01-31 Grifols Therapeutics Inc Métodos, composiciones y kits para determinar el virus de la inmunodeficiencia humana (vih)?.
ES2626488T3 (es) 2011-07-19 2017-07-25 Wave Life Sciences Pte. Ltd. Procedimientos para la síntesis de ácidos nucleicos funcionalizados
WO2013016321A1 (en) 2011-07-26 2013-01-31 Elitech Holding B.V. Minor groove binder phosphoramidites and methods of use
PT2643461T (pt) 2011-09-07 2016-07-19 Grifols Therapeutics Inc Métodos, composições e kits para determinação do vírus da hepatite a
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US9428535B2 (en) 2011-10-03 2016-08-30 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
JP2015502365A (ja) 2011-12-12 2015-01-22 オンコイミューニン，インコーポレイティド オリゴヌクレオチドのイン−ビボ送達
SMT202200355T1 (it) 2011-12-16 2022-11-18 Modernatx Inc Composizioni di mrna modificato
WO2013113326A1 (en) 2012-01-31 2013-08-08 Curevac Gmbh Pharmaceutical composition comprising a polymeric carrier cargo complex and at least one protein or peptide antigen
WO2013148147A1 (en) 2012-03-26 2013-10-03 The U.S.A., As Represented By The Secretary Dept. Of Health And Human Services Dna methylation analysis for the diagnosis, prognosis and treatment of adrenal neoplasms
EP2834260A4 (en) 2012-04-02 2016-08-10 Moderna Therapeutics Inc MODIFIED POLYNUCLEOTIDES FOR THE PREPARATION OF MEMBRANE PROTEINS
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2850092B1 (en) 2012-04-09 2017-03-01 Ionis Pharmaceuticals, Inc. Tricyclic nucleic acid analogs
WO2013154799A1 (en) 2012-04-09 2013-10-17 Isis Pharmaceuticals, Inc. Tricyclic nucleosides and oligomeric compounds prepared therefrom
US10202642B2 (en) 2012-05-02 2019-02-12 Ibis Biosciences, Inc. DNA sequencing
US10584377B2 (en) 2012-05-02 2020-03-10 Ibis Biosciences, Inc. DNA sequencing
US10458915B2 (en) 2012-05-18 2019-10-29 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US10245229B2 (en) 2012-06-08 2019-04-02 Translate Bio, Inc. Pulmonary delivery of mRNA to non-lung target cells
BR112015000723A2 (pt) 2012-07-13 2017-06-27 Shin Nippon Biomedical Laboratories Ltd adjuvante de ácido nucléico quiral
ES2940887T3 (es) 2012-07-13 2023-05-12 Wave Life Sciences Ltd Método de preparación de oligonucleótidos quirales
ES2862073T3 (es) 2012-07-13 2021-10-06 Wave Life Sciences Ltd Grupo auxiliar asimétrico
EP2885312B1 (en) 2012-08-15 2025-09-03 Ionis Pharmaceuticals, Inc. Method of preparing oligomeric compounds using modified capping protocols
CN104781271B (zh) 2012-08-20 2018-07-06 加利福尼亚大学董事会 具有生物可逆的基团的多核苷酸
WO2014030066A2 (en) 2012-08-22 2014-02-27 Bernitz Mats Nilsson Methods for identifying nucleic acid sequences
EA034342B1 (ru) * 2012-09-04 2020-01-29 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Соединение, нуклеиновая кислота, вещество, несущее метку, и способ обнаружения
IN2015DN02893A (cg-RX-API-DMAC7.html) 2012-10-04 2015-09-11 Univ Leland Stanford Junior
US9029335B2 (en) 2012-10-16 2015-05-12 Isis Pharmaceuticals, Inc. Substituted 2′-thio-bicyclic nucleosides and oligomeric compounds prepared therefrom
DK2909339T3 (en) 2012-10-18 2018-08-13 Idexx Lab Inc Kits comprehensive controls for nucleic acid amplification
WO2014072832A2 (en) 2012-10-18 2014-05-15 Oslo Universitetstssykehus Hf Biomarkers for cervical cancer
EP2922554B1 (en) 2012-11-26 2022-02-23 ModernaTX, Inc. Terminally modified rna
DK2951191T3 (en) 2013-01-31 2019-01-14 Ionis Pharmaceuticals Inc PROCEDURE FOR MANUFACTURING OLIGOMERIC COMPOUNDS USING MODIFIED CLUTCH PROTOCOLS
PL2970456T3 (pl) 2013-03-14 2022-01-31 Translate Bio, Inc. Sposoby i kompozycje do dostarczania przeciwciał kodowanych mrna
MX2015012865A (es) 2013-03-14 2016-07-21 Shire Human Genetic Therapies Metodos para purificacion de arn mensajero.
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
HUE050394T2 (hu) 2013-05-01 2020-11-30 Ionis Pharmaceuticals Inc Apolipoprotein(a) expressziójának módosítására szolgáló eljárások és készítmények
EP3004131B1 (en) 2013-06-05 2020-07-22 AM Chemicals Llc Phosphoramidite building blocks for sugar-conjugated oligonucleotides
US9069358B2 (en) 2013-06-24 2015-06-30 Biolytic Lab Performance, Inc. System for controlling and optimizing reactions in solid phase synthesis of small molecules
KR102207770B1 (ko) 2013-08-05 2021-01-26 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
ES2747762T3 (es) 2013-08-21 2020-03-11 Curevac Ag Vacuna contra el virus respiratorio sincitial (RSV)
PL3035955T3 (pl) 2013-08-21 2020-03-31 Curevac Ag Kompozycja i szczepionka do leczenia raka płuca
JP2016538829A (ja) 2013-10-03 2016-12-15 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 低密度リポタンパク質受容体をコードするポリヌクレオチド
US11162096B2 (en) 2013-10-14 2021-11-02 Ionis Pharmaceuticals, Inc Methods for modulating expression of C9ORF72 antisense transcript
WO2015061246A1 (en) 2013-10-21 2015-04-30 Isis Pharmaceuticals, Inc. Method for solution phase detritylation of oligomeric compounds
EP3871696B1 (en) 2013-10-22 2025-03-19 Translate Bio, Inc. Lipid formulations for delivery of messenger rna
CN105658800A (zh) 2013-10-22 2016-06-08 夏尔人类遗传性治疗公司 Mrna的cns递送及其用途
ES2954366T3 (es) 2013-10-22 2023-11-21 Translate Bio Inc Terapia de ácido ribonucleico mensajero para la deficiencia de argininosuccinato sintetasa
EP3574923A1 (en) 2013-10-22 2019-12-04 Translate Bio, Inc. Mrna therapy for phenylketonuria
ES2806575T3 (es) 2013-11-01 2021-02-18 Curevac Ag ARN modificado con propiedades inmunoestimuladoras disminuidas
CA2930693A1 (en) 2013-11-15 2015-05-21 The Board Of Trustees Of The Leland Stanford Junior Unversity Methods of treating heart failure with agonists of hypocretin receptor 2
EP3071588A4 (en) 2013-11-21 2017-08-02 Somalogic, Inc. Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
CN106061488B (zh) 2013-12-02 2021-04-09 菲奥医药公司 癌症的免疫治疗
ES2959628T3 (es) 2013-12-20 2024-02-27 Fraunhofer Ges Forschung Moléculas de ácido nucleico RNAPII-140 que confieren resistencia a plagas de coleópteros
BR102014031844A2 (pt) 2013-12-20 2015-10-06 Dow Agrosciences Llc ras oposto (rop) e moléculas de ácido nucleico relacionadas que conferem resistência a pragas de coleópteros e hemípteros
EP3090053B1 (en) 2013-12-30 2018-11-21 CureVac AG Artificial nucleic acid molecules
JP6704850B2 (ja) 2013-12-30 2020-06-03 キュアバック アーゲー 人工核酸分子
JP6584414B2 (ja) 2013-12-30 2019-10-02 キュアバック アーゲー 人工核酸分子
WO2015108047A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
CN113278617A (zh) 2014-01-16 2021-08-20 波涛生命科学有限公司 手性设计
WO2015142910A1 (en) 2014-03-17 2015-09-24 Isis Pharmaceuticals, Inc. Bicyclic carbocyclic nucleosides and oligomeric compounds prepared therefrom
RU2702838C2 (ru) 2014-03-19 2019-10-11 Ионис Фармасьютикалз, Инк. Композиции для модуляции экспрессии атаксина 2
US10006027B2 (en) 2014-03-19 2018-06-26 Ionis Pharmaceuticals, Inc. Methods for modulating Ataxin 2 expression
WO2015148247A1 (en) 2014-03-24 2015-10-01 Shire Human Genetic Therapies, Inc. Mrna therapy for the treatment of ocular diseases
SG11201608109TA (en) 2014-04-01 2016-10-28 Ionis Pharmaceuticals Inc Compositions for modulating sod-1 expression
WO2015149944A2 (en) 2014-04-01 2015-10-08 Curevac Gmbh Polymeric carrier cargo complex for use as an immunostimulating agent or as an adjuvant
US10221416B2 (en) 2014-04-24 2019-03-05 Ionis Pharmaceuticals, Inc. Oligomeric compounds comprising alpha-beta-constrained nucleic acid
MX373952B (es) 2014-04-25 2020-07-13 Shire Human Genetic Therapies Métodos de purificación de arn mensajero.
WO2015168108A2 (en) 2014-04-28 2015-11-05 Rxi Pharmaceuticals Corporation Methods for treating cancer using nucleic targeting mdm2 or mycn
US9926556B2 (en) 2014-04-28 2018-03-27 Ionis Pharmaceuticals, Inc. Linkage modified oligomeric compounds
KR102375480B1 (ko) 2014-04-30 2022-03-16 애질런트 테크놀로지스, 인크. 인 보호기 및 이의 제조 방법 및 용도
EP3137115B1 (en) 2014-05-01 2020-10-14 Ionis Pharmaceuticals, Inc. Method for synthesis of reactive conjugate clusters
CN106232804B (zh) 2014-05-01 2019-10-25 Ionis制药公司 用于调节补体因子b表达的组合物和方法
NZ725789A (en) 2014-05-07 2018-04-27 Dow Agrosciences Llc Dre4 nucleic acid molecules that confer resistance to coleopteran pests
EP3143022B1 (en) 2014-05-16 2021-12-22 Life Technologies Corporation Carbopyronone compounds useful as diagnostic adjuvants
CN106659731A (zh) 2014-05-30 2017-05-10 夏尔人类遗传性治疗公司 用于递送核酸的可生物降解脂质
CN106459133B (zh) 2014-06-10 2019-12-06 安捷伦科技有限公司 用于“z核苷酸”的保护基团及其方法
JP6599373B2 (ja) 2014-06-24 2019-10-30 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド 核酸の送達のための立体化学的に濃縮した組成物
CN114344275A (zh) 2014-07-02 2022-04-15 川斯勒佰尔公司 信使rna的包封
US10093967B2 (en) 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
US10900039B2 (en) 2014-09-05 2021-01-26 Phio Pharmaceuticals Corp. Methods for treating aging and skin disorders using nucleic acids targeting Tyr or MMP1
SG11201701128YA (en) 2014-09-12 2017-03-30 Genentech Inc Cysteine engineered antibodies and conjugates
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
US10308947B2 (en) 2014-10-17 2019-06-04 The Penn State Research Foundation Methods and compositions for multiplex RNA guided genome editing and other RNA technologies
WO2016090323A1 (en) 2014-12-05 2016-06-09 Prelude, Inc. Dcis recurrence and invasive breast cancer
AU2015357562B2 (en) 2014-12-05 2021-10-21 Translate Bio, Inc. Messenger RNA therapy for treatment of articular disease
JP2017538419A (ja) 2014-12-12 2017-12-28 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 抗生物質耐性菌を検出するための方法および組成物
EP3230468B1 (en) 2014-12-12 2020-09-16 ELITechGroup, Inc. Methods and kits for detecting antibiotic resistant bacteria
DK3708668T3 (da) 2014-12-12 2022-09-26 Curevac Ag Kunstigt nukleinsyremolekyle til forbedret proteinudtrykkelse
US20160194658A1 (en) 2014-12-22 2016-07-07 Dow Agrosciences Llc Nucampholin nucleic acid molecules to control coleopteran insect pests
US9688707B2 (en) 2014-12-30 2017-06-27 Ionis Pharmaceuticals, Inc. Bicyclic morpholino compounds and oligomeric compounds prepared therefrom
WO2016107877A1 (en) 2014-12-30 2016-07-07 Curevac Ag Artificial nucleic acid molecules
US10793855B2 (en) 2015-01-06 2020-10-06 Ionis Pharmaceuticals, Inc. Compositions for modulating expression of C9ORF72 antisense transcript
US10538763B2 (en) 2015-01-16 2020-01-21 Ionis Pharmaceuticals, Inc. Compounds and methods for modulation of DUX4
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
WO2016137923A1 (en) 2015-02-23 2016-09-01 Ionis Pharmaceuticals, Inc. Method for solution phase detritylation of oligomeric compounds
US11129844B2 (en) 2015-03-03 2021-09-28 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating MECP2 expression
US20160264991A1 (en) 2015-03-13 2016-09-15 Dow Agrosciences Llc Rna polymerase i1 nucleic acid molecules to control insect pests
AU2016233135B2 (en) 2015-03-19 2021-07-08 Translate Bio, Inc. mRNA therapy for pompe disease
WO2016167780A1 (en) 2015-04-16 2016-10-20 Ionis Pharmaceuticals, Inc. Compositions for modulating expression of c9orf72 antisense transcript
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
BR102016012010A2 (pt) 2015-05-29 2020-03-24 Dow Agrosciences Llc Molécula de ácido nucleico, de ácido ribonucleico (rna) e de ácido ribonucleico de filamento duplo (dsrna), usos de célula, planta e semente, produto primário, bem como métodos para controlar uma população de pragas coleópteras e/ou hemípteras, para melhorar o rendimento de uma cultura, e para produzir uma célula vegetal transgênica e uma planta transgênica
GB201511470D0 (en) 2015-06-30 2015-08-12 Cellcall Kft HPV detection method
KR20180026739A (ko) 2015-07-06 2018-03-13 알엑스아이 파마슈티칼스 코포레이션 슈퍼옥시드 디스뮤타제 1 (sod1)을 표적화하는 핵산 분자
US10808247B2 (en) 2015-07-06 2020-10-20 Phio Pharmaceuticals Corp. Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach
MA43072A (fr) 2015-07-22 2018-05-30 Wave Life Sciences Ltd Compositions d'oligonucléotides et procédés associés
EP3331557B1 (en) 2015-08-04 2021-04-07 Duke University Genetically encoded intrinsically disordered stealth polymers for delivery and methods of using same
SG10201913629VA (en) 2015-08-28 2020-03-30 Curevac Ag Artificial nucleic acid molecules
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
WO2017053781A1 (en) 2015-09-25 2017-03-30 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating ataxin 3 expression
EP3365446A4 (en) 2015-10-19 2019-06-26 Phio Pharmaceuticals Corp. SMALL SELF-LEANING NUCLEIC ACID COMPOUNDS TURNED AGAINST LONG NON-CODING RNA
EP4582098A3 (en) 2015-11-09 2025-12-24 CureVac SE Optimized nucleic acid molecules
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
WO2017096395A1 (en) 2015-12-04 2017-06-08 Ionis Pharmaceuticals, Inc. Methods of treating breast cancer
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
AU2016381174A1 (en) 2015-12-31 2018-05-31 Ionis Pharmaceuticals, Inc. Methods for reducing Ataxin-2 expression
AU2017205462A1 (en) 2016-01-05 2018-06-07 Ionis Pharmaceuticals, Inc. Methods for reducing LRRK2 expression
WO2017161168A1 (en) 2016-03-16 2017-09-21 Ionis Pharmaceuticals, Inc. Modulation of dyrk1b expression
AU2017234678A1 (en) 2016-03-16 2018-08-16 Ionis Pharmaceuticals, Inc. Methods of modulating KEAP1
ES2844180T3 (es) 2016-04-08 2021-07-21 Translate Bio Inc Acido nucleico codificante multimérico y usos del mismo
WO2017180420A1 (en) 2016-04-11 2017-10-19 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
US11467156B2 (en) 2016-06-01 2022-10-11 Duke University Nonfouling biosensors
AU2017283479B2 (en) 2016-06-13 2023-08-17 Translate Bio, Inc. Messenger RNA therapy for the treatment of ornithine transcarbamylase deficiency
CA3023514A1 (en) 2016-06-17 2017-12-21 Ionis Pharmaceuticals, Inc. Modulation of gys1 expression
EP3484908A4 (en) 2016-07-15 2020-04-08 AM Chemicals Llc NON-NUCLEOSIDIC SOLID CARRIERS AND PHOSPHORAMIDITE BUILDING BLOCKS FOR OLIGONUCLEOTID SYNTHESIS
CN109963871A (zh) 2016-08-05 2019-07-02 豪夫迈·罗氏有限公司 具有激动活性的多价及多表位抗体以及使用方法
CA3033867A1 (en) 2016-08-17 2018-02-22 Solstice Biologics, Ltd. Polynucleotide constructs
US10907156B2 (en) 2016-08-19 2021-02-02 Fundación Para La Investigación Biomédica Del Hospital Univ. Ramón Y Cajal MiR-127 agents for use in the treatment of renal fibrosis
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation NOVO SYNTHESIZED NUCLEIC ACID BANKS
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR20190064600A (ko) 2016-09-23 2019-06-10 듀크 유니버시티 Lcst 거동을 갖는 비구조화된 비-반복적 폴리펩티드
WO2018067900A1 (en) 2016-10-06 2018-04-12 Ionis Pharmaceuticals, Inc. Method of conjugating oligomeric compounds
JOP20190104A1 (ar) 2016-11-10 2019-05-07 Ionis Pharmaceuticals Inc مركبات وطرق لتقليل التعبير عن atxn3
WO2018089790A1 (en) 2016-11-10 2018-05-17 Translate Bio, Inc. Improved ice-based lipid nanoparticle formulation for delivery of mrna
CA3040728A1 (en) 2016-11-10 2018-05-17 Dow Agrosciences Llc Cytochrome b (cytb) nucleic acid molecules that control pathogens
JP7560946B2 (ja) 2016-11-10 2024-10-03 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの皮下送達
AU2017357758B2 (en) 2016-11-10 2023-11-16 Translate Bio, Inc. Improved process of preparing mRNA-loaded lipid nanoparticles
EP3548620A4 (en) 2016-12-02 2020-07-22 Cold Spring Harbor Laboratory MODULATION OF THE EXPRESSION OF LNC05
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3342780A1 (en) 2016-12-30 2018-07-04 Dow AgroSciences LLC Pre-mrna processing factor 8 (prp8) nucleic acid molecules to control insect pests
US11648200B2 (en) 2017-01-12 2023-05-16 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
EP3586255B1 (en) 2017-02-22 2025-01-15 Twist Bioscience Corporation Nucleic acid based data storage
ES2925083T3 (es) 2017-02-27 2022-10-13 Translate Bio Inc Métodos de purificación de ARN mensajero
WO2018157133A1 (en) 2017-02-27 2018-08-30 Translate Bio, Inc. Methods for purification of messenger rna
MA47824A (fr) 2017-03-07 2020-01-15 Translate Bio Inc Administration polyanionique d'acides nucléiques
JP7145517B2 (ja) 2017-03-08 2022-10-03 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 分析物の検出
WO2018165564A1 (en) 2017-03-09 2018-09-13 Ionis Pharmaceuticals, Inc. Morpholino modified oligomeric compounds
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11554097B2 (en) 2017-05-15 2023-01-17 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
MA49138A (fr) 2017-05-16 2020-03-25 Translate Bio Inc Traitement de la fibrose kystique par administration d'arnm à codons optimisés codant pour la cftr
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3638716B1 (en) 2017-06-12 2022-01-05 Translate Bio, Inc. Poly(phosphoesters) for delivery of nucleic acids
WO2018236849A1 (en) 2017-06-19 2018-12-27 Translate Bio, Inc. Messenger rna therapy for the treatment of friedreich's ataxia
US11597744B2 (en) 2017-06-30 2023-03-07 Sirius Therapeutics, Inc. Chiral phosphoramidite auxiliaries and methods of their use
EP3658168A4 (en) 2017-06-30 2021-07-14 Duke University ORDER AND DISORDER AS A DESIGN PRINCIPLE FOR STIMULUS SENSITIVE BIOPOLYMER NETWORKS
AU2018318231A1 (en) 2017-08-18 2020-02-13 Ionis Pharmaceuticals, Inc. Modulation of the notch signaling pathway for treatment of respiratory disorders
WO2019051173A1 (en) 2017-09-08 2019-03-14 Ionis Pharmaceuticals, Inc. MODULATORS OF SMAD7 EXPRESSION
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
AU2018334273B2 (en) 2017-09-15 2025-04-10 Brandeis University Multiplex production and barcoding of genetically engineered cells
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
TWI809004B (zh) 2017-11-09 2023-07-21 美商Ionis製藥公司 用於降低snca表現之化合物及方法
EP3727428A1 (en) 2017-12-20 2020-10-28 Translate Bio, Inc. Improved composition and methods for treatment of ornithine transcarbamylase deficiency
WO2019126641A2 (en) 2017-12-21 2019-06-27 Ionis Pharmaceuticals, Inc. Modulation of frataxin expression
IL275818B2 (en) 2018-01-04 2024-10-01 Twist Bioscience Corp Dna-based digital information storage
BR112020014425A2 (pt) 2018-01-15 2020-12-29 Ionis Pharmaceuticals, Inc. Moduladores de expressão de dnm2
WO2019147954A1 (en) 2018-01-26 2019-08-01 Duke University Albumin binding peptide-drug (aibiped) conjugates and methods of making and using same
WO2019152802A1 (en) 2018-02-02 2019-08-08 Translate Bio, Inc. Cationic polymers
EP3759127A4 (en) 2018-03-02 2022-03-30 Ionis Pharmaceuticals, Inc. COMPOUNDS AND METHODS FOR MODULATING AMYLOID BETA PRECURSOR PROTEIN
TWI840345B (zh) 2018-03-02 2024-05-01 美商Ionis製藥公司 Irf4表現之調節劑
WO2019183440A1 (en) 2018-03-22 2019-09-26 Ionis Pharmaceuticals, Inc. Methods for modulating fmr1 expression
MX2020010721A (es) 2018-04-11 2020-11-06 Ionis Pharmaceuticals Inc Moduladores de la expresion de ezh2.
BR112020018905A2 (pt) 2018-04-13 2021-02-09 Bristol-Myers Squibb Company reagentes com base em fósforo (v), processos para a preparação dos mesmos e seu uso na fabricação de compostos organofosforados (v) estéreo definidos
US12257308B2 (en) 2018-04-30 2025-03-25 Duke University Stimuli-responsive PEG-like polymer-based drug delivery platform
US11866788B2 (en) 2018-05-08 2024-01-09 Sanford Burnham Prebys Medical Discovery Institute Role of PVT1 in the diagnosis and treatment of MYC-driven cancer
CR20200604A (es) 2018-05-09 2021-02-09 Ionis Pharmaceuticals Inc Compuestos y métodos para reducir de la expresión de atxn3
CR20200605A (es) 2018-05-09 2021-01-29 Ionis Pharmaceuticals Inc Compuestos y métodos para la reducción de la expresión de fxi
WO2019222277A1 (en) 2018-05-15 2019-11-21 Translate Bio, Inc. Subcutaneous delivery of messenger rna
EP3794008A1 (en) 2018-05-16 2021-03-24 Translate Bio, Inc. Ribose cationic lipids
IL278771B2 (en) 2018-05-18 2025-09-01 Twist Bioscience Corp Polynucleotides, reagents and methods for nucleic acid hybridization
CN112437767B (zh) 2018-05-24 2023-10-27 川斯勒佰尔公司 硫酯阳离子脂质
AU2019278813B2 (en) 2018-05-30 2023-12-07 Translate Bio, Inc. Cationic lipids comprising a steroidal moiety
US20210213140A1 (en) 2018-05-30 2021-07-15 Translate Bio, Inc. Phosphoester Cationic Lipids
CN112533909B (zh) 2018-05-30 2024-11-15 川斯勒佰尔公司 维生素阳离子脂质
EP3801467A1 (en) 2018-05-30 2021-04-14 Translate Bio, Inc. Messenger rna vaccines and uses thereof
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
AU2019287635A1 (en) 2018-06-14 2020-12-17 Ionis Pharmaceuticals, Inc. Compounds and methods for increasing STMN2 expression
TWI833770B (zh) 2018-06-27 2024-03-01 美商Ionis製藥公司 用於減少 lrrk2 表現之化合物及方法
WO2020001793A1 (en) 2018-06-29 2020-01-02 Eberhard-Karls-Universität Tübingen Artificial nucleic acids for rna editing
WO2020018558A1 (en) 2018-07-17 2020-01-23 Aronora, Inc. Methods for safely reducing thrombopoietin
CN120514877A (zh) 2018-07-23 2025-08-22 川斯勒佰尔公司 信使rna的干粉制剂
MX2021000922A (es) 2018-07-25 2021-03-31 Ionis Pharmaceuticals Inc Compuestos y metodos para reducir la expresion de la atxn2.
EP3829622A4 (en) 2018-08-02 2022-05-11 Duke University DOUBLE AGONIST FUSION PROTEINS
CN112930396B (zh) 2018-08-24 2024-05-24 川斯勒佰尔公司 用于纯化信使rna的方法
CN112839639A (zh) 2018-08-29 2021-05-25 川斯勒佰尔公司 制备负载mRNA的脂质纳米颗粒的改进方法
US20220187301A1 (en) 2018-09-14 2022-06-16 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
EP3849617A1 (en) 2018-09-14 2021-07-21 Translate Bio, Inc. Composition and methods for treatment of methylmalonic acidemia
WO2020081933A1 (en) 2018-10-19 2020-04-23 Translate Bio, Inc. Pumpless encapsulation of messenger rna
CA3115119A1 (en) 2018-11-08 2020-05-14 Translate Bio, Inc. Methods and compositions for messenger rna purification
EP3877444A1 (en) 2018-11-09 2021-09-15 Translate Bio, Inc. Multi-peg lipid compounds
EP3876994A2 (en) 2018-11-09 2021-09-15 Translate Bio, Inc. Peg lipidoid compounds
WO2020097384A1 (en) 2018-11-09 2020-05-14 Translate Bio, Inc. 2,5-dioxopiperazine lipids with intercalated ester, thioester, disulfide and anhydride moieities
WO2020097511A2 (en) 2018-11-09 2020-05-14 Translate Bio, Inc. Messenger rna therapy for treatment of ocular diseases
AU2019378763B2 (en) 2018-11-12 2025-06-26 Translate Bio, Inc. Methods for inducing immune tolerance
TW202028222A (zh) 2018-11-14 2020-08-01 美商Ionis製藥公司 Foxp3表現之調節劑
BR112021008967A2 (pt) 2018-11-15 2021-08-17 Ionis Pharmaceuticals, Inc. moduladores da expressão de irf5
AU2019384557B2 (en) 2018-11-21 2025-07-17 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of nebulized mRNA encoding CFTR
CA3119976A1 (en) 2018-11-21 2020-05-28 Translate Bio, Inc. Cationic lipid compounds
AU2019384181B2 (en) 2018-11-21 2025-04-10 Ionis Pharmaceuticals, Inc. Compounds and methods for reducing prion expression
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS
US20220087935A1 (en) 2019-01-07 2022-03-24 Translate Bio, Inc. Composition and Methods for Treatment of Primary Ciliary Dyskinesia
WO2020160453A1 (en) 2019-01-31 2020-08-06 Ionis Pharmaceuticals, Inc. Modulators of yap1 expression
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
US11279932B2 (en) 2019-02-27 2022-03-22 Ionis Pharmaceuticals, Inc. Modulators of MALAT1 expression
MX2021011916A (es) 2019-03-29 2021-10-26 Ionis Pharmaceuticals Inc Compuestos y metodos para modular ube3a-ats.
EP3956303A1 (en) 2019-04-18 2022-02-23 Translate Bio, Inc. Cystine cationic lipids
EP3959195B1 (en) 2019-04-22 2023-11-08 Translate Bio, Inc. Thioester cationic lipids
WO2020227085A1 (en) 2019-05-03 2020-11-12 Translate Bio, Inc. Di-thioester cationic lipids
SG11202112574RA (en) 2019-05-14 2021-12-30 Translate Bio Inc Improved process of preparing mrna-loaded lipid nanoparticles
KR20220029556A (ko) 2019-05-15 2022-03-08 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
EP3976593A1 (en) 2019-05-31 2022-04-06 Translate Bio, Inc. Macrocyclic lipids
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN114401942B (zh) 2019-06-21 2024-01-26 川斯勒佰尔公司 三(羟甲基)甲基甘氨酸和柠檬酸脂质
WO2020257611A1 (en) 2019-06-21 2020-12-24 Translate Bio, Inc. Cationic lipids comprising an hydroxy moiety
AU2020311364B2 (en) 2019-07-08 2025-12-18 Translate Bio, Inc. Improved mRNA-loaded lipid nanoparticles and processes of making the same
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
MX2022000934A (es) 2019-07-23 2022-02-14 Translate Bio Inc Composiciones estables de nanoparticulas lipidicas cargadas de arnm y procesos de fabricacion.
ES3048358T3 (en) 2019-07-26 2025-12-10 Ionis Pharmaceuticals Inc Compounds and methods for modulating gfap
ES3029073T3 (en) 2019-07-30 2025-06-23 Translate Bio Inc Treatment of cystic fibrosis by delivery of nebulized mrna encoding cftr
CN114555621B (zh) 2019-08-15 2025-10-28 Ionis制药公司 键修饰的寡聚化合物及其用途
WO2021061829A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
JP7752615B2 (ja) 2019-12-20 2025-10-10 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの直腸送達
JP2023508881A (ja) 2019-12-20 2023-03-06 トランスレイト バイオ， インコーポレイテッド Ｍｒｎａ担持脂質ナノ粒子を調製する改善されたプロセス
WO2021142245A1 (en) 2020-01-10 2021-07-15 Translate Bio, Inc. Compounds, pharmaceutical compositions and methods for modulating expression of muc5b in lung cells and tissues
CN115443338A (zh) 2020-02-10 2022-12-06 翻译生物公司 用于信使rna纯化的方法和组合物
US11969480B2 (en) 2020-02-25 2024-04-30 Translate Bio, Inc. Processes of preparing mRNA-loaded lipid nanoparticles
BR112022016238A2 (pt) 2020-02-28 2022-10-11 Ionis Pharmaceuticals Inc Compostos e métodos para modular smn2
WO2021202694A1 (en) 2020-04-01 2021-10-07 Translate Bio, Inc. Phenolic acid lipid based cationic lipids
KR20230023612A (ko) 2020-04-02 2023-02-17 마이레큘, 인크. 조작된 올리고뉴클레오티드를 사용한 표적화된 억제
IL297435A (en) 2020-05-01 2022-12-01 Ionis Pharmaceuticals Inc Compounds and methods for modulating atxn1
WO2021226463A1 (en) 2020-05-07 2021-11-11 Translate Bio, Inc. Composition and methods for treatment of primary ciliary dyskinesia
WO2021231697A1 (en) 2020-05-14 2021-11-18 Translate Bio, Inc. Peg lipidoid compounds
CA3178965A1 (en) 2020-05-15 2021-11-18 Christian COBAUGH Messenger rna encoding cas9 for use in genome-editing systems
AU2021273502A1 (en) 2020-05-15 2023-02-02 Translate Bio, Inc. Lipid nanoparticle formulations for mRNA delivery
EP4172338A4 (en) 2020-06-29 2025-06-11 Ionis Pharmaceuticals, Inc. COMPOUNDS AND METHODS FOR MODULATING PLP1
WO2022006527A1 (en) 2020-07-02 2022-01-06 Maritime Therapeutics, Inc. Compositions and methods for reverse gene therapy
WO2022026589A1 (en) 2020-07-28 2022-02-03 Ionis Pharmaceuticals, Inc. Compounds and methods for reducing app expression
EP4192476A4 (en) 2020-08-07 2024-09-18 Ionis Pharmaceuticals, Inc. COMPOUNDS AND METHODS FOR MODULATING SCN2A
CN116457027A (zh) 2020-09-23 2023-07-18 翻译生物公司 基于哌嗪的阳离子脂质
AU2021347814A1 (en) 2020-09-23 2023-06-08 Translate Bio, Inc. Tes-based cationic lipids
JP2023544167A (ja) 2020-10-01 2023-10-20 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製のための方法
US20230372440A1 (en) 2020-10-06 2023-11-23 Translate Bio, Inc. Improved process and formulation of lipid nanoparticles
CN116490166A (zh) 2020-10-12 2023-07-25 翻译生物公司 制备加载mRNA的脂质纳米颗粒的改进方法
US20220133631A1 (en) 2020-10-12 2022-05-05 Translate Bio, Inc. Process of preparing ice-based lipid nanoparticles
WO2022078569A1 (en) 2020-10-12 2022-04-21 Eberhard Karls Universität Tübingen Artificial nucleic acids for rna editing
TW202233232A (zh) 2020-11-06 2022-09-01 法商賽諾菲公司 遞送mRNA疫苗的脂質奈米顆粒
ES2990026T3 (es) 2020-11-18 2024-11-28 Ionis Pharmaceuticals Inc Compuestos y métodos para modular la expresión del angiotensinógeno
TW202242111A (zh) 2020-12-18 2022-11-01 美商Ionis製藥公司 用於調節因子ｘｉｉ之化合物及方法
WO2022178438A1 (en) 2021-02-22 2022-08-25 Duke University Non-immunogenic poegma-aptamer conjugates
EP4288519A4 (en) 2021-02-26 2025-03-26 The Regents of University of California Assembled three-dimensional cultures of human neurons and glia and their use
TW202309002A (zh) 2021-04-15 2023-03-01 美商轉譯生技公司 基於「古德」緩衝液的陽離子脂質
US20220364078A1 (en) 2021-05-14 2022-11-17 Crispr Therapeutics Ag Mrna large scale synthesis and purification
US20240279828A1 (en) 2021-05-31 2024-08-22 Genscript Usa Inc. Electrografted films for dna synthesis
US20240262878A1 (en) 2021-06-07 2024-08-08 The Board Of Trustees Of The University Of Illinois Photoactivatable gasdermin proteins
BR112023026050A2 (pt) 2021-06-18 2024-03-05 Ionis Pharmaceuticals Inc Compostos e métodos para reduzir expressão de ifnar1
CA3224175A1 (en) 2021-06-18 2022-12-22 Sanofi Multivalent influenza vaccines
MX2024000754A (es) 2021-07-14 2024-04-18 Mirecule Inc Oligonucleotidos y composiciones de los mismos para trastornos neuromusculares.
JP2024534216A (ja) 2021-09-02 2024-09-18 サノフイ Ｒｎａアフィニティー精製のための組成物および方法
EP4395933A1 (en) 2021-09-03 2024-07-10 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
CN118302155A (zh) 2021-10-05 2024-07-05 赛诺菲 用于冷冻和冷冻干燥脂质纳米颗粒（lnp）的方法及其获得的lnp
WO2023059412A1 (en) 2021-10-05 2023-04-13 Microsoft Technology Licensing, Llc Regulation of dna synthesis by nucleotides linked to protecting groups
US20250230511A1 (en) 2021-10-29 2025-07-17 Sana Biotechnology, Inc. Methods and reagents for amplifying viral vector nucleic acid products
EP4426343A1 (en) 2021-11-05 2024-09-11 Sanofi Respiratory syncytial virus rna vaccine
EP4429713A1 (en) 2021-11-10 2024-09-18 Translate Bio, Inc. Composition and methods for treatment of primary ciliary dyskinesia
US20230310571A1 (en) 2021-11-30 2023-10-05 Sanofi Pasteur Inc. Human metapneumovirus vaccines
CA3242439A1 (en) 2021-12-17 2023-06-22 Sanofi Lyme disease rna vaccine
JP2025504430A (ja) 2022-01-17 2025-02-12 サノフイ 脂質化合物及びその使用
JP2025509507A (ja) 2022-03-16 2025-04-11 トランスレイト バイオ， インコーポレイテッド 非対称ピペラジン系カチオン性脂質
CA3253952A1 (en) 2022-03-28 2023-10-05 F. Hoffmann-La Roche Ag SYNTHESIS OF PHOSPHORAMIDITES BASED ON ISOMERICALLY PURE POLYOLS
US20250263385A1 (en) 2022-04-13 2025-08-21 Sanofi "Good" buffer-based cationic lipids
CA3256624A1 (en) 2022-05-06 2023-11-09 Sanofi SIGNAL SEQUENCES FOR NUCLEIC ACID-BASED VACCINES
WO2023242425A1 (en) 2022-06-17 2023-12-21 Sanofi Compositions and methods for circular rna affinity purification
CN119487130A (zh) 2022-06-28 2025-02-18 豪夫迈·罗氏有限公司 具有大斯托克斯位移的荧光染料
CA3263324A1 (en) 2022-07-29 2024-02-01 Sanofi Pasteur Inc. ETHANOL-FREE MRNA PURIFICATION PROCESSES
WO2024044108A1 (en) 2022-08-22 2024-02-29 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Vaccines against coronaviruses
CA3268249A1 (en) 2022-09-23 2024-03-28 Ionis Pharmaceuticals, Inc. COMPOUNDS AND METHODS FOR REDUCING MECP2 EXPRESSION
EP4360657A1 (en) 2022-10-28 2024-05-01 Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Cajal Mir-326 for the treatment of cancer
WO2024094876A1 (en) 2022-11-04 2024-05-10 Sanofi Methods for messenger rna tailing
TW202434283A (zh) 2022-11-04 2024-09-01 法商賽諾菲公司 呼吸道融合病毒rna疫苗接種
WO2024099575A1 (en) 2022-11-11 2024-05-16 Eberhard Karls Universität Tübingen Artificial nucleic acids for site-directed editing of a target rna
JP2025538523A (ja) 2022-11-21 2025-11-28 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの乾燥粉末製剤の組成物及びその使用方法
JP2025541298A (ja) 2022-12-15 2025-12-18 サノフィ パスツール インコーポレイテッド ｍＲＮＡ組換えキャッピング酵素
EP4638415A1 (en) 2022-12-22 2025-10-29 Sanofi Pasteur Inc. Bis-ester and amide cationic lipids
EP4623103A1 (en) 2023-01-06 2025-10-01 DNA Script Methods for obtaining correctly assembled nucleic acids
GB202300915D0 (en) 2023-01-20 2023-03-08 Sisaf Ltd mRNA COMPOSITIONS
AU2024228998A1 (en) 2023-03-02 2025-10-16 Sanofi Pasteur Compositions for use in treatment of chlamydia
KR20250169625A (ko) 2023-04-17 2025-12-03 사노피 파스퇴르 인크 재구성 가능한 건조 분말 제형 및 이의 사용 방법
WO2024228121A1 (en) 2023-05-02 2024-11-07 The University Of Tokyo Long interspersed nuclear element r2 for target-specific gene delivery
US12502424B2 (en) 2023-05-05 2025-12-23 Sanofi Pasteur Inc. Compositions for use in treatment of acne
WO2024231565A1 (en) 2023-05-10 2024-11-14 Sanofi Combination respiratory mrna vaccines
WO2024256457A1 (en) 2023-06-12 2024-12-19 Sanofi Tricine and citric acid-based cationic lipids with aromatic head groups
WO2024256453A1 (en) 2023-06-12 2024-12-19 Sanofi Sterol-based cationic lipids with aromatic head groups
TW202513534A (zh) 2023-06-12 2025-04-01 法商賽諾菲公司 三(羥甲基)甲基甘胺酸與檸檬酸基底的陽離子脂質
WO2025003755A1 (en) 2023-06-28 2025-01-02 Sanofi Helper lipids for nucleic acid delivery
WO2025003760A1 (en) 2023-06-28 2025-01-02 Sanofi Sterol analogs in lipid nanoparticle formulations
AR133127A1 (es) 2023-06-28 2025-08-27 Sanofi Sa Lípidos ionizables a base de dianhidrohexitol para el suministro de ácidos nucleicos
WO2025017202A2 (en) 2023-07-19 2025-01-23 Sanofi Porphyromonas gingivalis antigenic constructs
TW202527960A (zh) 2023-09-06 2025-07-16 美商賽諾菲巴斯德公司 經修飾ｂ型流感血球凝集素多肽及核酸及其用途
WO2025061967A1 (en) 2023-09-20 2025-03-27 Sanofi "good" buffer-based cationic lipids for nucleic acid delivery
WO2025085519A1 (en) 2023-10-16 2025-04-24 Typewriter Therapeutics, Inc. R2 retrotransposons for gene writing
WO2025085682A1 (en) 2023-10-18 2025-04-24 Amgen Inc. Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development
EP4553174A1 (en) 2023-11-08 2025-05-14 Consejo Superior De Investigaciones Científicas Methods to determine the thymic function by droplet digital pcr system
WO2025134071A1 (en) 2023-12-22 2025-06-26 Sanofi Malic and glutaric acid based ionizable lipids
WO2025141521A1 (en) 2023-12-29 2025-07-03 Sanofi Lipids having dendritic moieties
WO2025163146A1 (en) 2024-01-31 2025-08-07 Sanofi Methods for ethanol-free mrna purification
WO2025196065A1 (en) 2024-03-20 2025-09-25 Sanofi Novel homocysteine based lipids and their use for delivery of nucleic acids
WO2025210592A1 (en) 2024-04-05 2025-10-09 Sanofi Pasteur Inc. Muscle cell lnp-mrna quality control assays
WO2025217194A1 (en) 2024-04-09 2025-10-16 Typewriter Therapeutics, Inc. Repeat dosing of retrotransposons
WO2025250906A1 (en) 2024-05-30 2025-12-04 Aera Therapeutics, Inc. Process for preparation of targeted lipid nanoparticles

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3534017A (en) * 1967-03-14 1970-10-13 Kyowa Hakko Kogyo Kk Process for the preparation of nucleoside-5'-diphosphates and triphosphates and mono- and oligo-nucleotidyl-nucleoside-5'-diphosphates and triphosphates
EP0035719B1 (en) * 1980-02-29 1989-09-20 University Patents, Inc. Process for producing modified inorganic polymers, their use in producing polynucleotides, and a reagent useful in these processes

Also Published As

Publication number Publication date
CA1203237A (en) 1986-04-15
ATE12236T1 (de) 1985-04-15
EP0061746A1 (en) 1982-10-06
EP0061746B1 (en) 1985-03-20
JPS6328439B2 (cg-RX-API-DMAC7.html) 1988-06-08
JPS57176998A (en) 1982-10-30
US4415732A (en) 1983-11-15
JPS63179889A (ja) 1988-07-23
JPS6365677B2 (cg-RX-API-DMAC7.html) 1988-12-16
AU8199782A (en) 1982-09-30
MX157501A (es) 1988-11-28
DE3262598D1 (en) 1985-04-25

Similar Documents

Publication Publication Date Title
AU551324B2 (en) Phosphoramidite derivatives of nucleotides and their use in preparation of oligonucleotides
EP0007508A3 (en) Acylphosphinoxide compounds, their preparation, their use, photopolymerisable printing compositions and process for the preparation of relief forms
JPS55150826A (en) Aeration chamber
GR78442B (cg-RX-API-DMAC7.html)
AR231983A1 (es) Procedimiento para la preparacion de nuevos derivados de 5,6,7,8-tetrahidro-4h-(tieno,pirrolo o furo)(2,3-d)azepina
FI832004A7 (fi) Menetelmä 1-sulfo-2-oksoatsetidiini- johdannaisten valmistamiseksi.
IT8024824A0 (it) Procedimento per la preparazione di poliolefine.
PH20267A (en) Preparation for the treatment of herpes zoster and other herpes infections, as well as method for manufacture thereof
ES501729A0 (es) Procedimiento para la preparacion selectiva de 2-cloro-1,1, 1,2,3,3,3-heptafluoropropano
ZA804178B (en) Process for the preparation of 2,6-dialkylcyclohexylamines
IT8019504A0 (it) Procedimento per la preparazione selettiva di isosorbite-5-nitrato.
DE3062141D1 (en) Process for the preparation of 1,2-dichloroethane
IT7925761A0 (it) Processo per la preparazione di l-alfa-glicerilfosforilcolina.
DE3065004D1 (en) Benzothiazole derivatives, process for their preparation and their therapeutical uses
IL66164A0 (en) Imidazolylpropionitriles,process for the manufacture of these compounds and their use as biocidal agents
DE2966043D1 (en) Process for the preparation of oside derivatives, derivatives so obtained and their biological applications
DE3369132D1 (en) Process for the preparation of isomelamines, and their use
PT72086A (en) Process for the preparation of 5,10-dihydro-imydazoi(2,1-b)quinazolines with pharmaceutical effect
GB2048754B (en) Tool-supporting bench
PT71606A (en) Process for the preparation of benzodiazepine derivatives
DE3067531D1 (en) Process for the preparation of 1,4-benzodiazepines
DE3062357D1 (en) Decahydroquinolinol derivatives, process for their preparation and their therapeutical use
NL189907B (nl) Werkwijze voor de bereiding van natriumtrifosfaat.
AR230439A1 (es) Proceso para la preparacion de 2-isopropilaminopirimidina-n-oxido
GB2056453B (en) Process for the preparation of nitrosobenzene

Legal Events

Date Code Title Description
MK14 Patent ceased section 143(a) (annual fees not paid) or expired