ATE319857T1 - Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese - Google Patents

Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese

Info

Publication number
ATE319857T1
ATE319857T1 AT99967297T AT99967297T ATE319857T1 AT E319857 T1 ATE319857 T1 AT E319857T1 AT 99967297 T AT99967297 T AT 99967297T AT 99967297 T AT99967297 T AT 99967297T AT E319857 T1 ATE319857 T1 AT E319857T1
Authority
AT
Austria
Prior art keywords
nucleic acid
kit
acid sequencing
individual molecules
polymerase synthesis
Prior art date
Application number
AT99967297T
Other languages
English (en)
Inventor
John G K Williams
Original Assignee
Li Cor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26809573&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE319857(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Li Cor Inc filed Critical Li Cor Inc
Application granted granted Critical
Publication of ATE319857T1 publication Critical patent/ATE319857T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D209/00Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04Indoles; Hydrogenated indoles
    • C07D209/10Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/12Radicals substituted by oxygen atoms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]
AT99967297T 1998-12-14 1999-12-13 Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese ATE319857T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11207898P 1998-12-14 1998-12-14
US11549699P 1999-01-11 1999-01-11

Publications (1)

Publication Number Publication Date
ATE319857T1 true ATE319857T1 (de) 2006-03-15

Family

ID=26809573

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99967297T ATE319857T1 (de) 1998-12-14 1999-12-13 Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese

Country Status (8)

Country Link
US (10) US6255083B1 (de)
EP (1) EP1141409B2 (de)
JP (2) JP4638043B2 (de)
AT (1) ATE319857T1 (de)
AU (2) AU772281B2 (de)
CA (1) CA2355816C (de)
DE (1) DE69930310T3 (de)
WO (2) WO2000036152A1 (de)

Families Citing this family (394)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
CN1251609A (zh) 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
DK1159453T3 (da) * 1999-03-10 2008-10-06 Asm Scient Inc Fremgangsmåde til direkte nukleinsyresekventering
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1681356B1 (de) 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Methode zur Nukleinsäurensequenzierung
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6190473B1 (en) 1999-08-12 2001-02-20 The Boenig Company Titanium alloy having enhanced notch toughness and method of producing same
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6458538B1 (en) * 1999-12-14 2002-10-01 Ptc Therapeutics, Inc. Methods of assaying for compounds that inhibit premature translation termination and nonsense-mediated RNA decay
DE10017823B4 (de) * 2000-04-10 2004-08-26 Till I.D. Gmbh Mikroskopische Beleuchtungsvorrichtung
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
WO2001094609A1 (en) 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
CA2415125A1 (en) * 2000-07-03 2002-01-10 Applera Corporation Polynucleotide sequence assay
AU2001282881B2 (en) * 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
US6358679B1 (en) * 2000-08-24 2002-03-19 Pe Corporation (Ny) Methods for external controls for nucleic acid amplification
JP3638516B2 (ja) * 2000-09-28 2005-04-13 株式会社日立製作所 核酸検出方法および核酸検出キット
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
WO2002029003A2 (en) 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002231933A1 (en) * 2001-01-30 2002-08-12 Solexa Ltd. The preparation of polynucleotide arrays
WO2002061391A2 (en) * 2001-01-31 2002-08-08 The University Of Tennessee Research Corporation Methods for detecting interaction of molecules with surface-bound reagents
US7083987B2 (en) 2001-02-05 2006-08-01 Activx Biosciences, Inc. Activity based probe analysis
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
EP1370690B1 (de) 2001-03-16 2012-03-14 Kalim Mir Arrays und verfahren zur deren verwendung
WO2002086088A2 (en) * 2001-04-24 2002-10-31 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7118907B2 (en) * 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US20040018502A1 (en) * 2001-06-14 2004-01-29 Yoshihiko Makino Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment
US7141370B2 (en) * 2001-07-03 2006-11-28 The Board Of Trustees Of The Leland Stanford Junior University Bioluminescence regenerative cycle (BRC) for nucleic acid quantification
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
EP1281769A1 (de) * 2001-08-01 2003-02-05 Aventis Pharma S.A. Screening-Verfahren für Inhibitoren von Enzymen der Folat Synthese
GB0119719D0 (en) * 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US7560254B2 (en) * 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US7244566B2 (en) * 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
US7256019B2 (en) * 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
ATE458067T1 (de) * 2001-08-29 2010-03-15 Ge Healthcare Bio Sciences Markierte nukleosidpolyphosphate
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
MXPA04003926A (es) * 2001-10-24 2004-06-18 Singulex Inc Metodos para detectar haplotipos geneticos por interaccion con sondas.
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
DE10162536A1 (de) * 2001-12-19 2003-07-17 Gnothis Holding Sa Ecublens Evaneszenz-basierendes Multiplex-Sequenzierungsverfahren
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
WO2003087302A2 (en) * 2002-04-12 2003-10-23 Stratagene Dual-labeled nucleotides
WO2003089670A2 (en) * 2002-04-22 2003-10-30 Lawler Joseph F Jr Reagents for monitoring nuclei acid amplification and methods of using same
US7291461B2 (en) * 2002-06-21 2007-11-06 Ptc Therapeutics, Inc. Methods for identifying small molecules that modulate premature translation termination and nonsense mRNA decay
DE10232139A1 (de) * 2002-07-04 2004-01-22 Technische Universität Dresden Mit Nukleinsäuren und Nukleinsäurederivaten beschichteter metallischer Gegenstand, Verfahren zu dessen Herstellung und dessen Verwendung
AU2003252136A1 (en) * 2002-07-24 2004-02-09 Ptc Therapeutics, Inc. METHODS FOR IDENTIFYING SMALL MOLEDULES THAT MODULATE PREMATURE TRANSLATION TERMINATION AND NONSENSE MEDIATED mRNA DECAY
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3795577A1 (de) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modifizierte nukleotide
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
ATE547535T1 (de) * 2002-08-29 2012-03-15 Ge Healthcare Bio Sciences Nukleosidpolyphosphate mit blockiertem terminalem phosphat
JP4643262B2 (ja) * 2002-08-29 2011-03-02 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 検体検出法
WO2004020604A2 (en) * 2002-08-29 2004-03-11 Amersham Biosciences Corp Allele specific primer extension
AU2003267583A1 (en) * 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US20050164205A1 (en) * 2002-11-19 2005-07-28 Singulex, Inc. Charge and mass tags for detection and analysis
WO2005054258A2 (en) * 2003-12-02 2005-06-16 Innate Pharma New class of gamma delta t cells activators and use thereof
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
JP4896708B2 (ja) * 2003-02-05 2012-03-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 新規リンカー類を有する末端リン酸標識ヌクレオチド類
CA2513690A1 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
WO2004072304A1 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Nucleic acid amplification
CN103396933B (zh) * 2003-02-26 2016-04-20 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
WO2004092331A2 (en) * 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
EP1647592B1 (de) * 2003-06-30 2009-03-11 Panasonic Corporation Verfahren zur modifizierung einer nukleotidkette
US20100291637A1 (en) * 2003-06-30 2010-11-18 Panasonic Corporation Method for modifying nucleotide chain
CN1294276C (zh) * 2003-07-22 2007-01-10 厦门大学 检测核酸的等长双链特异性探针
US20090088982A1 (en) * 2003-07-31 2009-04-02 Fukushima Noelle H Co-detection of single polypeptide and polynucleotide molecules
JP4230430B2 (ja) * 2003-09-25 2009-02-25 富士通株式会社 被検体評価装置および被検体評価方法
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
KR100500610B1 (ko) * 2003-10-29 2005-07-11 한국전기연구원 형광 현미경 및 이를 사용한 관측 방법
US20050095599A1 (en) * 2003-10-30 2005-05-05 Pittaro Richard J. Detection and identification of biopolymers using fluorescence quenching
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
KR100643192B1 (ko) 2003-12-02 2006-11-10 홍종인 신규 이핵 금속 착물 및 이를 이용한 파이로포스페이트의분석방법
US20050147979A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US8323886B2 (en) * 2004-06-18 2012-12-04 Wlodek Mandecki Fluorescence methods of detecting a protein-elongation related complex and corollary sequence information
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
US20060019259A1 (en) * 2004-07-22 2006-01-26 Joyce Timothy H Characterization of biopolymers by resonance tunneling and fluorescence quenching
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
WO2006036388A2 (en) * 2004-08-23 2006-04-06 U.S. Genomics, Inc. Systems and methods for detecting and analyzing polymers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
GB2423819B (en) 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
WO2007008246A2 (en) 2004-11-12 2007-01-18 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for dna and other molecules
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US9695472B2 (en) * 2005-03-04 2017-07-04 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP2008533167A (ja) 2005-03-15 2008-08-21 アプレラ コーポレイション 分析物の検出のための、抗体の代替抗原系の使用
US7709249B2 (en) * 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7584947B2 (en) * 2005-05-20 2009-09-08 The Boeing Company Reconfigurable workpiece support fixture
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
US20070009382A1 (en) * 2005-07-05 2007-01-11 William Bedingham Heating element for a rotating multiplex fluorescence detection device
US7527763B2 (en) 2005-07-05 2009-05-05 3M Innovative Properties Company Valve control system for a rotating multiplex fluorescence detection device
CA2616433A1 (en) * 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US20070048773A1 (en) * 2005-07-29 2007-03-01 Applera Corporation Detection of polyphosphate using fluorescently labeled polyphosphate acceptor substrates
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US20070141593A1 (en) * 2005-08-22 2007-06-21 Lee Linda G Apparatus, system, and method using immiscible-fluid-discrete-volumes
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
US20070154921A1 (en) * 2005-12-16 2007-07-05 Applera Corporation Method and System for Phase-Locked Sequencing
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
JPWO2007097443A1 (ja) 2006-02-20 2009-07-16 国立大学法人 北海道大学 Dna塩基配列を決定する方法
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
US20080009007A1 (en) * 2006-06-16 2008-01-10 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
US7519514B2 (en) * 2006-07-14 2009-04-14 Agilent Technologies, Inc. Systems and methods for removing noise from spectral data
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
AU2007289057C1 (en) 2006-09-01 2014-01-16 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2653861B1 (de) 2006-12-14 2014-08-13 Life Technologies Corporation Verfahren zur Nukleinsäure-Sequenzierung mittels großer FET-Arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
US8017104B2 (en) * 2007-02-28 2011-09-13 Carestream Health, Inc. Large stoke shift dye used for optical imaging
US8906354B2 (en) 2007-02-28 2014-12-09 Bruker Biospin Corporation Loaded latex optical molecular imaging probes containing lipophilic large stokes shift dyes
US20080220537A1 (en) * 2007-03-07 2008-09-11 Pacific Biosciences Of California, Inc. Substrates and methods for selective immobilization of active molecules
US20080234143A1 (en) * 2007-03-23 2008-09-25 Pacific Biosciences Of California, Inc. Mechanical methods for producing substrates having selectively immobilized molecules
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
AU2008282862B2 (en) * 2007-07-26 2014-07-31 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
US8233572B2 (en) * 2007-09-25 2012-07-31 Qualcomm Incorporated Interference mitigation for impulse-based communication
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
EP2203566A4 (de) * 2007-09-28 2011-02-09 Pacific Biosciences California Fehlerfreie dna-amplifikation für klonale sequenzierung
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
EP3561494B1 (de) * 2007-12-06 2022-03-02 Genalyte, Inc. VERFAHREN ZUM IDENTIFIZIEREN EINER SEQUENZ VON NUKLEOTIDEN IN EINER UNBEKANNTEN SPEZIES VON NUKLEINSÄURE
UND VORRICHTUNG ZUR DURCHFÜHRUNG DES VERFAHRENS
CA2711560A1 (en) 2008-01-10 2009-07-16 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
US20100029498A1 (en) * 2008-02-04 2010-02-04 Andreas Gnirke Selection of nucleic acids by solution hybridization to oligonucleotide baits
WO2009100382A1 (en) * 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
EP2255014A4 (de) * 2008-02-12 2011-05-18 Pacific Biosciences California Zusammensetzungen und verfahren zur verwendung bei analysereaktionen
JP5570731B2 (ja) * 2008-02-28 2014-08-13 旭化成ファーマ株式会社 ピロリン酸の測定方法
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US20100035253A1 (en) * 2008-03-19 2010-02-11 Intelligent Bio-Systems, Inc. Methods And Compositions For Incorporating Nucleotides
US7973146B2 (en) * 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
US8143030B2 (en) * 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8236499B2 (en) * 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8153375B2 (en) 2008-03-28 2012-04-10 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US9637380B2 (en) 2008-03-31 2017-05-02 Pacific Biosciences Of California, Inc. Nanoscale apertures having islands of functionality
EP2881736B1 (de) * 2008-03-31 2017-06-07 Pacific Biosciences of California, Inc. Verfahren und Zusammensetzungen zur Ladung von Einzelpolymerase
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2274446B1 (de) * 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Systeme und verfahren für polymeraseenzymreaktionen in zwei langsamen schritten
ES2614078T3 (es) * 2008-03-31 2017-05-29 Pacific Biosciences Of California, Inc. Generación de polimerasas modificadas para precisión mejorada en secuenciación de una única molécula
CA2720511A1 (en) * 2008-04-01 2009-10-08 Biosearch Technologies, Inc. Stabilized nucleic acid dark quencher-fluorophore probes
EP2276849B1 (de) * 2008-04-24 2014-12-03 3M Innovative Properties Company Untersuchung von nukleinsäureamplifikationskurven anhand von wavelet-transformation
CN102124018A (zh) * 2008-04-29 2011-07-13 生命科技公司 可维持从核苷酸模板酶促合成双链核苷酸的非天然聚合酶底物及其使用的方法
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
EP2307577B1 (de) 2008-06-25 2015-06-03 Life Technologies Corporation Verfahren zur analytmessung mithilfe grosser fet-arrays
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
WO2010027484A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
CA3063032A1 (en) 2008-09-16 2010-03-25 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US20100184045A1 (en) * 2008-09-23 2010-07-22 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP3572796B8 (de) 2008-10-27 2023-01-18 Genalyte, Inc. Biosensoren basierend auf optischer sondierung und abtastung
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
CA2746632C (en) * 2008-12-11 2020-06-30 Pacific Biosciences Of California, Inc. Characterization of modified nucleic acids
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US8236532B2 (en) * 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
WO2010111691A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Conjugates of biomolecules to nanoparticles
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8501405B2 (en) * 2009-04-27 2013-08-06 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
US8609421B2 (en) * 2009-06-12 2013-12-17 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
EP2475787A2 (de) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequenzbestimmung durch anwendung entgegengesetzter kräfte
WO2011038241A1 (en) * 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
US8547533B2 (en) * 2009-12-28 2013-10-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Composite probes and use thereof in super resolution methods
US8500979B2 (en) * 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
US8518643B2 (en) * 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) * 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
HUE026666T2 (en) 2010-04-05 2016-07-28 Prognosys Biosciences Inc Spatially encoded biological assays
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) * 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
WO2012003359A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
US8432149B2 (en) 2010-06-30 2013-04-30 Life Technologies Corporation Array column integrator
CN103154718B (zh) 2010-06-30 2015-09-23 生命科技公司 感测离子的电荷堆积电路和方法
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
US8653567B2 (en) 2010-07-03 2014-02-18 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US8715932B2 (en) 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
EP2606154B1 (de) 2010-08-20 2019-09-25 Integenx Inc. Integriertes analysesystem
US8669374B2 (en) 2010-08-25 2014-03-11 Gene Shen Functionalized cyanine dyes (PEG)
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
US9618475B2 (en) 2010-09-15 2017-04-11 Life Technologies Corporation Methods and apparatus for measuring analytes
US8685324B2 (en) 2010-09-24 2014-04-01 Life Technologies Corporation Matched pair transistor circuits
WO2012054784A1 (en) 2010-10-20 2012-04-26 Li-Cor, Inc. Fluorescent imaging with substituted cyanine dyes
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
CA2816995C (en) 2010-11-05 2019-12-31 THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, a body corporate and politic Optical analyte detection systems and methods of use
WO2012088341A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
CA2827880A1 (en) 2011-02-23 2012-08-30 Eve Biomedical, Inc. Rotation-dependent transcriptional sequencing systems and methods of using
EP2689028B1 (de) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolierung von polymerase-nukleinsäurekomplexen und deren ladung auf substrate
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
EP2694709B1 (de) 2011-04-08 2016-09-14 Prognosys Biosciences, Inc. Peptidkonstrukte und testsysteme dafür
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
EP3670523B1 (de) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh-unblockierte, schnell photospaltbare endgruppennukleotide und ihre verwendung in nukleinsäuresequenzierungsverfahren
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
CA2858036C (en) 2011-12-15 2017-08-22 Intel Corporation Diamond electrode nanogap transducers
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
WO2013100949A1 (en) 2011-12-28 2013-07-04 Intel Corporation Nanogap transducers with selective surface immobilization sites
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9062091B2 (en) 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
DE102012102983A1 (de) 2012-04-05 2013-10-10 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen eines kritischen Winkels eines Anregungslichtstrahls
WO2013163207A1 (en) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification of 5-methyl-c in nucleic acid templates
EP2844774B1 (de) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna-sequenzierung
US10584377B2 (en) 2012-05-02 2020-03-10 Ibis Biosciences, Inc. DNA sequencing
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
US10458915B2 (en) 2012-05-18 2019-10-29 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
GB2517875A (en) 2012-06-08 2015-03-04 Pacific Biosciences California Modified base detection with nanopore sequencing
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
WO2013192106A1 (en) 2012-06-17 2013-12-27 Pacific Biosciences Of California, Inc. Filter architecture for analytical devices
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
CN105308062A (zh) 2013-02-20 2016-02-03 伊芙生物医学股份有限公司 用于基于纳米结构的核酸测序的方法和组合物
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US20140264472A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
KR102230831B1 (ko) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014143637A1 (en) 2013-03-15 2014-09-18 The Board Of Trustees Of The University Of Illinois Methods and compositions for enhancing immunoassays
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US9128044B2 (en) 2013-03-15 2015-09-08 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
CA2916660C (en) 2013-06-25 2022-05-17 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
WO2015006344A1 (en) * 2013-07-08 2015-01-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for synthesizing selectively labeled rna
EP3030683B1 (de) 2013-08-05 2022-03-02 Pacific Biosciences of California, Inc. Geschützte fluoreszierende reagenzverbindungen
CN110283888B (zh) 2013-08-19 2021-06-22 卓异生物公司 用于单分子检测的测定及其应用
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
CN105723222B (zh) 2013-10-23 2019-01-22 吉尼亚科技公司 使用纳米孔的高速分子感测
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
AU2014348306B2 (en) 2013-11-17 2020-11-12 Quantum-Si Incorporated Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens
CN105873681B (zh) 2013-11-18 2019-10-11 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
US10421997B2 (en) 2014-02-25 2019-09-24 Roche Diagnostics Oeprations, Inc. Molecular dynamic sequencing
AU2015236392A1 (en) * 2014-03-24 2016-10-20 Genia Technologies, Inc. Chemical methods for producing tagged nucleotides
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
CA2957543A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
EP3471402B1 (de) 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrierte vorrichtung zur temporären gruppierung von empfangenen photonen
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
MX2017001808A (es) 2014-08-08 2018-02-08 Quantum Si Inc Dispositivo integrado con fuente de luz externa para el sondeo, deteccion y analisis de moleculas.
EP3552690A1 (de) 2014-10-22 2019-10-16 IntegenX Inc. Systeme und verfahren zur automatisierten vorbereitung, verarbeitung und analyse von proben
US10190162B2 (en) 2014-10-23 2019-01-29 Complete Genomics, Inc. Signal confinement sequencing (SCS) and nucleotide analogues for signal confinement sequencing
US11218410B2 (en) * 2014-11-10 2022-01-04 Marvell Asia Pte, Ltd. Hybrid wildcard match table
US11943142B2 (en) * 2014-11-10 2024-03-26 Marvell Asia Pte, LTD Hybrid wildcard match table
US10344325B2 (en) 2014-11-11 2019-07-09 Life Technologies Corporation Thiolated nucleotide analogues for nucleic acid synthesis
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
EP3258952A4 (de) 2015-02-04 2018-12-05 Pacific Biosciences Of California, Inc. Multimere geschützte fluoreszierende reagenzien
AU2016219943A1 (en) 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
DK3283499T3 (da) * 2015-04-15 2022-08-08 Biosearch Tech Inc Dual quencher probes
CN106153806B (zh) * 2015-05-11 2018-01-19 上海市计量测试技术研究院 三磷酸腺苷的纯度检测方法
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
EP3332032B1 (de) 2015-08-06 2023-12-27 Pacific Biosciences Of California, Inc. Integrierte schaltungen zur selektiven adressierung spärlich angeordneter, elektronischer messvorrichtungen
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
WO2017087696A1 (en) 2015-11-18 2017-05-26 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
CN110343753B (zh) * 2015-11-19 2022-06-21 赛纳生物科技(北京)有限公司 一种磷酸修饰荧光团的核苷酸分子测序方法
EP3350350B1 (de) 2015-11-19 2021-04-14 Pacific Biosciences of California, Inc. Verbindungen und systeme zur verbesserung der signaldetektion
WO2017084580A1 (en) 2015-11-19 2017-05-26 Peking University Methods for obtaining and correcting biological sequence information
US10781483B2 (en) 2015-11-20 2020-09-22 Pacific Biosciences Of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
CN117024498A (zh) 2015-11-20 2023-11-10 加利福尼亚太平洋生物科学股份有限公司 受保护的染料标记的试剂
AU2017219894B2 (en) 2016-02-17 2021-12-09 Tesseract Health, Inc. Sensor and device for lifetime imaging and detection applications
WO2018052877A1 (en) * 2016-09-16 2018-03-22 The Board Of Regents Of The University Of Texas System Redox activatable fluorescent sensors
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
KR20230052995A (ko) 2016-12-19 2023-04-20 퀀텀-에스아이 인코포레이티드 시퀀싱 반응을 위한 중합 효소
CN110168732B (zh) 2016-12-22 2024-03-08 宽腾矽公司 具有直接合并像素的整合式光电侦测器
CA3059211A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
US11001885B2 (en) 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
EP3658682A4 (de) 2017-07-24 2021-06-02 Quantum-si Incorporated Markierte reaktantzusammensetzungen mit hoher intensität und verfahren zur sequenzierung
WO2019067635A1 (en) * 2017-09-27 2019-04-04 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CN112534803A (zh) 2018-06-22 2021-03-19 宽腾矽公司 具有不同检测时间的电荷存储仓的集成光电检测器
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
JP2022509868A (ja) * 2018-11-30 2022-01-24 ジェネインフォセック、インコーポレイテッド ランダムオリゴヌクレオチドの生成方法およびその配列決定方法
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3914603A1 (de) 2019-01-23 2021-12-01 Quantum-Si Incorporated Hochintensiv markierte reaktantzusammensetzungen und verfahren zur sequenzierung
CA3130693A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
CN110609020B (zh) * 2019-08-15 2021-10-01 济南大学 基于回文分子信标检测atp的生物传感器及其制备方法和应用
CN114829669A (zh) 2019-10-11 2022-07-29 宽腾矽公司 气相表面改性
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055185A1 (de) 2019-11-08 2022-09-14 10X Genomics, Inc. Räumlich markierte analytfänger für analyt-multiplexing
DK3891300T3 (da) 2019-12-23 2023-05-22 10X Genomics Inc Fremgangsmåder til rumlig analyse ved anvendelse af rna-template-ligering
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
JP2023512634A (ja) 2020-01-21 2023-03-28 クアンタム-エスアイ インコーポレイテッド C末端の選択的標識のための化合物および方法
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US20230091256A1 (en) 2020-02-28 2023-03-23 Curevac Netherlands B.V. Hidden Frame Neoantigens
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (de) 2020-06-10 2023-04-19 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220162680A1 (en) * 2020-11-20 2022-05-26 Illumina, Inc. Compositions and methods for sequencing using fluorophores and quenchers or donors
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
CN112552363B (zh) * 2020-12-24 2022-03-18 山东大学 一种偶联抗坏血酸的核苷酸探针及其制备方法和应用
WO2022198068A1 (en) 2021-03-18 2022-09-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2023014366A1 (en) * 2021-08-05 2023-02-09 Hewlett-Packard Development Company, L.P. Test well having a bottom including a single opening
WO2023014363A1 (en) * 2021-08-05 2023-02-09 Hewlett-Packard Development Company, L.P. Polymerase chain reaction test well including magnetic portion
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023068931A1 (en) 2021-10-21 2023-04-27 Curevac Netherlands B.V. Cancer neoantigens
WO2023076772A1 (en) * 2021-11-01 2023-05-04 New England Biolabs, Inc. Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes
EP4174491A1 (de) * 2021-11-01 2023-05-03 New England Biolabs, Inc. Zusammensetzungen und verfahren zum detektieren von pyrophosphatprodukten aus enzymreaktionen mit pyridylazoanilinfarbstoffen
CN114317684B (zh) * 2021-12-15 2023-12-26 南京大学 一种基于tna分子的细胞内镁离子成像的方法

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4318981A (en) * 1980-04-24 1982-03-09 Miles Laboratories, Inc. Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5013831A (en) 1984-01-30 1991-05-07 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5849478A (en) 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の5−及び6−スクシニミジルカルボキシレ―ト異性体
US5273879A (en) 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
JP2955759B2 (ja) * 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450069A4 (en) 1989-10-25 1993-03-17 Nkk Corporation Method of continuously plating metal plates
EP0450060A1 (de) * 1989-10-26 1991-10-09 Sri International Dns-sequenzierung
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
RU1794088C (ru) * 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US6100099A (en) * 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB9210168D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
US5489508A (en) * 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
CA2155186A1 (en) 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
DE69435196D1 (de) * 1993-10-28 2009-04-23 Houston Advanced Res Ct Mikrofabriziertes poröses Durchflussgerät zur diskreten Bestimmung von Bindungsreaktionen
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
JP3175110B2 (ja) * 1994-02-07 2001-06-11 オーキッド・バイオサイエンシーズ・インコーポレイテッド リガーゼ/ポリメラーゼ媒体された単一ヌクレオチド多型のジェネティックビットアナリシスおよび遺伝子解析におけるその使用
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5635608A (en) 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5736318A (en) * 1995-03-17 1998-04-07 President And Fellows Of Harvard College Method and kit for evaluating human papillomavirus transformed cells
GB9509336D0 (en) * 1995-05-09 1995-06-28 Dynal As Chemical method
US5872243A (en) 1995-06-30 1999-02-16 Molecular Probes, Inc. Caged nucleotides
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
WO1997022719A1 (en) * 1995-12-18 1997-06-26 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US6099823A (en) * 1996-02-16 2000-08-08 Millennium Pharmaceuticals, Inc. Compositions and methods for the treatment and diagnosis of cardiovascular disease
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5846737A (en) 1996-07-26 1998-12-08 Molecular Probes, Inc. Conjugates of sulforhodamine fluorophores with enhanced fluorescence
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9716052D0 (en) * 1996-12-06 1997-10-01 Secr Defence Reaction vessels
US5942397A (en) * 1996-12-11 1999-08-24 Tarlov; Michael J. Surface immobilization of biopolymers
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998033939A1 (fr) 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
CN1251609A (zh) 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
US5842767A (en) * 1997-03-11 1998-12-01 Farlight Corporation Highly efficient illuminator and method of use thereof
ATE269908T1 (de) * 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
WO1998049344A1 (en) * 1997-04-28 1998-11-05 Lockheed Martin Energy Research Corporation Method and apparatus for analyzing nucleic acids
WO1999002544A1 (fr) * 1997-07-11 1999-01-21 The Institute Of Physical And Chemical Research Composes possedant une fonction de transfert d'energie et procede de sequencage des bases d'adn a l'aide de ces composes
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
DE69808661T3 (de) * 1997-07-28 2012-05-03 Gen-Probe Inc. Sequenzanalyse von nukleinsäuren
JP3813818B2 (ja) 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ オリゴヌクレオチドおよびdna分子のヌクレオチド配列の決定方法
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
AU772281B2 (en) * 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2004092331A2 (en) * 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7767400B2 (en) 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis

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