DE69930310D1 - Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese - Google Patents

Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese

Info

Publication number
DE69930310D1
DE69930310D1 DE69930310T DE69930310T DE69930310D1 DE 69930310 D1 DE69930310 D1 DE 69930310D1 DE 69930310 T DE69930310 T DE 69930310T DE 69930310 T DE69930310 T DE 69930310T DE 69930310 D1 DE69930310 D1 DE 69930310D1
Authority
DE
Germany
Prior art keywords
nucleic acid
kit
acid sequencing
individual molecules
polymerase synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69930310T
Other languages
English (en)
Other versions
DE69930310T3 (de
DE69930310T2 (de
Inventor
G Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pacific Biosciences of California Inc
Original Assignee
Li Cor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26809573&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69930310(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Li Cor Inc filed Critical Li Cor Inc
Application granted granted Critical
Publication of DE69930310D1 publication Critical patent/DE69930310D1/de
Publication of DE69930310T2 publication Critical patent/DE69930310T2/de
Publication of DE69930310T3 publication Critical patent/DE69930310T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/12—Radicals substituted by oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE69930310T 1998-12-14 1999-12-13 Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese Expired - Lifetime DE69930310T3 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11207898P 1998-12-14 1998-12-14
US112078P 1998-12-14
US11549699P 1999-01-11 1999-01-11
US115496P 1999-01-11
PCT/US1999/029585 WO2000036152A1 (en) 1998-12-14 1999-12-13 A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis

Publications (3)

Publication Number Publication Date
DE69930310D1 true DE69930310D1 (de) 2006-05-04
DE69930310T2 DE69930310T2 (de) 2006-12-28
DE69930310T3 DE69930310T3 (de) 2009-12-17

Family

ID=26809573

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69930310T Expired - Lifetime DE69930310T3 (de) 1998-12-14 1999-12-13 Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese

Country Status (8)

Country Link
US (10) US6232075B1 (de)
EP (1) EP1141409B2 (de)
JP (2) JP4638043B2 (de)
AT (1) ATE319857T1 (de)
AU (2) AU772281B2 (de)
CA (1) CA2355816C (de)
DE (1) DE69930310T3 (de)
WO (2) WO2000036151A1 (de)

Families Citing this family (397)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
JP2002537858A (ja) * 1999-03-10 2002-11-12 エーエスエム サイエンティフィック， インコーポレイテッド 核酸の配列を直接決定するための方法
EP1681356B1 (de) 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Methode zur Nukleinsäurensequenzierung
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6190473B1 (en) 1999-08-12 2001-02-20 The Boenig Company Titanium alloy having enhanced notch toughness and method of producing same
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6458538B1 (en) * 1999-12-14 2002-10-01 Ptc Therapeutics, Inc. Methods of assaying for compounds that inhibit premature translation termination and nonsense-mediated RNA decay
DE10017823B4 (de) * 2000-04-10 2004-08-26 Till I.D. Gmbh Mikroskopische Beleuchtungsvorrichtung
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
EP1287154A4 (de) 2000-06-07 2010-12-15 Pacific Biosciences California Ladungswechsel-nukleotide
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
WO2002002823A2 (en) * 2000-07-03 2002-01-10 Applera Corporation Polynucleotide sequence assay
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
EP1368460B1 (de) * 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Sequenzbestimmung in echtzeit
US6358679B1 (en) * 2000-08-24 2002-03-19 Pe Corporation (Ny) Methods for external controls for nucleic acid amplification
JP3638516B2 (ja) * 2000-09-28 2005-04-13 株式会社日立製作所 核酸検出方法および核酸検出キット
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7211414B2 (en) * 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
DE60222628T2 (de) * 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
WO2002061391A2 (en) * 2001-01-31 2002-08-08 The University Of Tennessee Research Corporation Methods for detecting interaction of molecules with surface-bound reagents
EP1364209A4 (de) 2001-02-05 2004-04-14 Activx Biosciences Inc Auf aktivität basierende sondenanalyse
US7297518B2 (en) * 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP2465943A3 (de) 2001-03-16 2012-10-03 Kalim Mir Lineare Polymeranzeige
WO2002086088A2 (en) * 2001-04-24 2002-10-31 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US20040018502A1 (en) * 2001-06-14 2004-01-29 Yoshihiko Makino Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment
AU2002367744A1 (en) * 2001-07-03 2003-10-27 The Board Of Trustees Of The Leland Stanford University Bioluminescence regenerative cycle (brc) for nucleic acid quantification
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
EP1281769A1 (de) * 2001-08-01 2003-02-05 Aventis Pharma S.A. Screening-Verfahren für Inhibitoren von Enzymen der Folat Synthese
GB0119719D0 (en) * 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US7560254B2 (en) * 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7256019B2 (en) * 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7244566B2 (en) * 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
CA2463420A1 (en) * 2001-10-24 2003-05-01 Singulex, Inc. Methods for detecting genetic haplotypes by interaction with probes
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
DE10162536A1 (de) * 2001-12-19 2003-07-17 Gnothis Holding Sa Ecublens Evaneszenz-basierendes Multiplex-Sequenzierungsverfahren
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
WO2003087302A2 (en) * 2002-04-12 2003-10-23 Stratagene Dual-labeled nucleotides
CA2482767A1 (en) * 2002-04-22 2003-10-30 Joseph F. Lawler, Jr. Reagents for monitoring nuclei acid amplification and methods of using same
US7291461B2 (en) * 2002-06-21 2007-11-06 Ptc Therapeutics, Inc. Methods for identifying small molecules that modulate premature translation termination and nonsense mRNA decay
DE10232139A1 (de) * 2002-07-04 2004-01-22 Technische Universität Dresden Mit Nukleinsäuren und Nukleinsäurederivaten beschichteter metallischer Gegenstand, Verfahren zu dessen Herstellung und dessen Verwendung
EP1543157A4 (de) * 2002-07-24 2006-11-15 Ptc Therapeutics Inc VERFAHREN ZUR IDENTIFIZIERUNG KLEINER, DIE VORZEITIGE TRANSLATIONSTERMINATION UND DEN NONSENSE-VERMITTELTEN mRNA-ABBAU MODULIERENDER MOLEKÜLE
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (de) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modifizierte nukleotide zur polynukleotidsequenzierung
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DK1546354T3 (da) * 2002-08-29 2010-08-16 Ge Healthcare Bio Sciences Analytdetektion
EP1546399B1 (de) * 2002-08-29 2012-02-29 GE Healthcare Bio-Sciences Corp. Nukleosidpolyphosphate mit blockiertem terminalem phosphat
JP4643263B2 (ja) * 2002-08-29 2011-03-02 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 対立遺伝子特異的プライマー伸長
AU2003267583A1 (en) * 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US20050164205A1 (en) * 2002-11-19 2005-07-28 Singulex, Inc. Charge and mass tags for detection and analysis
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
US20040152119A1 (en) * 2003-02-05 2004-08-05 Anup Sood Solid phase sequencing
ATE489479T1 (de) * 2003-02-05 2010-12-15 Ge Healthcare Bio Sciences Nukleinsäureamplifikation
EP1592779A4 (de) * 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Phosphat-endmarkierte nukleotide mit neuen linkern
EP2365095A1 (de) * 2003-02-26 2011-09-14 Callida Genomics, Inc. Zufallsarray DNA Analyse mittels Hybridisierung
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
WO2004092331A2 (en) * 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
WO2005014808A1 (ja) * 2003-06-30 2005-02-17 Matsushita Electric Industrial Co., Ltd. ヌクレオチド鎖修飾方法
US20100291637A1 (en) * 2003-06-30 2010-11-18 Panasonic Corporation Method for modifying nucleotide chain
CN1294276C (zh) * 2003-07-22 2007-01-10 厦门大学 检测核酸的等长双链特异性探针
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
JP4230430B2 (ja) * 2003-09-25 2009-02-25 富士通株式会社 被検体評価装置および被検体評価方法
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
KR100500610B1 (ko) * 2003-10-29 2005-07-11 한국전기연구원 형광 현미경 및 이를 사용한 관측 방법
US20050095599A1 (en) * 2003-10-30 2005-05-05 Pittaro Richard J. Detection and identification of biopolymers using fluorescence quenching
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1689758A2 (de) 2003-12-02 2006-08-16 Innate Pharma Neue klasse von aktivatoren von gamma-delta-t-zellen und deren verwendung
KR100643192B1 (ko) 2003-12-02 2006-11-10 홍종인 신규 이핵 금속 착물 및 이를 이용한 파이로포스페이트의분석방법
US20050147979A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US8323886B2 (en) * 2004-06-18 2012-12-04 Wlodek Mandecki Fluorescence methods of detecting a protein-elongation related complex and corollary sequence information
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
US20060019259A1 (en) * 2004-07-22 2006-01-26 Joyce Timothy H Characterization of biopolymers by resonance tunneling and fluorescence quenching
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
WO2006036388A2 (en) * 2004-08-23 2006-04-06 U.S. Genomics, Inc. Systems and methods for detecting and analyzing polymers
US7476503B2 (en) 2004-09-17 2009-01-13 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US9695472B2 (en) * 2005-03-04 2017-07-04 Intel Corporation Sensor arrays and nucleic acid sequencing applications
WO2006099537A2 (en) 2005-03-15 2006-09-21 Applera Corporation The use of antibody-surrogate antigen systems for detection of analytes
US7709249B2 (en) * 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7584947B2 (en) * 2005-05-20 2009-09-08 The Boeing Company Reconfigurable workpiece support fixture
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
US7527763B2 (en) 2005-07-05 2009-05-05 3M Innovative Properties Company Valve control system for a rotating multiplex fluorescence detection device
US20070009382A1 (en) * 2005-07-05 2007-01-11 William Bedingham Heating element for a rotating multiplex fluorescence detection device
WO2007014397A2 (en) * 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US20070048773A1 (en) * 2005-07-29 2007-03-01 Applera Corporation Detection of polyphosphate using fluorescently labeled polyphosphate acceptor substrates
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US9285297B2 (en) * 2005-08-22 2016-03-15 Applied Biosystems, Llc Device, system, and method for depositing processed immiscible-fluid-discrete-volumes
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
JP2009519717A (ja) * 2005-12-16 2009-05-21 アプレラ コーポレイション 位相を固定した配列決定のための方法およびシステム
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
JPWO2007097443A1 (ja) 2006-02-20 2009-07-16 国立大学法人 北海道大学 Ｄｎａ塩基配列を決定する方法
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
EP2029780A4 (de) * 2006-06-16 2010-03-31 Pacific Biosciences California Gesteuerte initiierung einer primärextension
US7519514B2 (en) * 2006-07-14 2009-04-14 Agilent Technologies, Inc. Systems and methods for removing noise from spectral data
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
WO2008028160A2 (en) 2006-09-01 2008-03-06 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (de) 2006-12-14 2014-11-19 Life Technologies Corporation Verfahren zur Sequenzierung einer Nukleinsäure mittels grosser FET-Arrays, geeignet zur Messung in einem begrenzten pH-Bereich
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
US8017104B2 (en) * 2007-02-28 2011-09-13 Carestream Health, Inc. Large stoke shift dye used for optical imaging
US8906354B2 (en) 2007-02-28 2014-12-09 Bruker Biospin Corporation Loaded latex optical molecular imaging probes containing lipophilic large stokes shift dyes
US20080220537A1 (en) * 2007-03-07 2008-09-11 Pacific Biosciences Of California, Inc. Substrates and methods for selective immobilization of active molecules
US20080234143A1 (en) * 2007-03-23 2008-09-25 Pacific Biosciences Of California, Inc. Mechanical methods for producing substrates having selectively immobilized molecules
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
EP2183388A4 (de) 2007-07-26 2010-09-08 Pacific Biosciences California Molekulares redundantes sequenzieren
US8233572B2 (en) * 2007-09-25 2012-07-31 Qualcomm Incorporated Interference mitigation for impulse-based communication
US8003330B2 (en) * 2007-09-28 2011-08-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
WO2009054922A1 (en) 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
ES2916498T3 (es) 2007-12-06 2022-07-01 Genalyte Inc Método para identificar una secuencia de nucleótidos en una especie desconocida de ácido nucleico; y dispositivo para llevar a cabo el método
CA2711560A1 (en) 2008-01-10 2009-07-16 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
EP2245198A1 (de) * 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selektion von nukleinsäuren durch lösungshybridisierung an oligonukleotidköder
EP2252625B1 (de) * 2008-02-07 2017-12-20 Pacific Biosciences of California, Inc. In linker eingebundene, cis-reaktive sauerstoffquencher
AU2009215193A1 (en) * 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
JP5570731B2 (ja) * 2008-02-28 2014-08-13 旭化成ファーマ株式会社 ピロリン酸の測定方法
US8883999B2 (en) * 2008-03-19 2014-11-11 Intelligent Bio Systems, Inc. Methods and solutions for inhibiting undesired cleaving of labels
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
CA2719747C (en) 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
ES2614078T3 (es) * 2008-03-31 2017-05-29 Pacific Biosciences Of California, Inc. Generación de polimerasas modificadas para precisión mejorada en secuenciación de una única molécula
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
AU2009251881B2 (en) * 2008-03-31 2015-03-26 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
EP2274446B1 (de) * 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Systeme und verfahren für polymeraseenzymreaktionen in zwei langsamen schritten
PT2757091T (pt) * 2008-04-01 2017-07-24 Biosearch Tech Inc Sondas de fluoróforo e supressor de fluorescência de ácido nucleico estabilizadas
CN102046807B (zh) * 2008-04-24 2014-11-26 3M创新有限公司 利用小波变换分析核酸扩增曲线
US8058414B2 (en) 2008-04-29 2011-11-15 Life Technologies Corporation Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
AU2009257369B2 (en) 2008-06-11 2015-03-26 Agilent Technologies, Inc. Nucleotides and nucleosides and methods for their use in DNA sequencing
CN102203282B (zh) 2008-06-25 2014-04-30 生命技术公司 使用大规模fet阵列测量分析物的方法和装置
US8198023B2 (en) * 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
AU2009288696A1 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Sequencing by cognate sampling
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
EP2331934B1 (de) 2008-09-16 2020-01-01 Pacific Biosciences of California, Inc. Analysegerät mit Nullmoden-Wellenleiter Substrat
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
WO2010039189A2 (en) * 2008-09-23 2010-04-08 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010036287A1 (en) * 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2347247B1 (de) 2008-10-27 2019-06-26 Genalyte, Inc. Auf optischer sondierung und optischem abtasten basierende biosensoren
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US9175341B2 (en) * 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
EP2607496B1 (de) 2008-12-23 2014-07-16 Illumina, Inc. Für Nukleinsäuresequenzierungsprotokolle geeignete Verfahren
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US20110014612A1 (en) 2009-03-27 2011-01-20 Life Technologies Corporation Polymerase compositions & methods
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8501405B2 (en) * 2009-04-27 2013-08-06 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
US8609421B2 (en) * 2009-06-12 2013-12-17 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
EP2475787A2 (de) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequenzbestimmung durch anwendung entgegengesetzter kräfte
WO2011038241A1 (en) * 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
WO2011090710A2 (en) * 2009-12-28 2011-07-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Composite probes and use thereof in super resolution methods
US8500979B2 (en) * 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
US8518643B2 (en) * 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US9482615B2 (en) * 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
TWI534431B (zh) 2010-04-28 2016-05-21 加州太平洋生物科學公司 形成具有官能性島狀區之奈米級孔洞的方法
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
CN103154718B (zh) 2010-06-30 2015-09-23 生命科技公司 感测离子的电荷堆积电路和方法
TWI539172B (zh) 2010-06-30 2016-06-21 生命技術公司 用於測試離子感測場效電晶體（ｉｓｆｅｔ）陣列之裝置及方法
WO2012003380A2 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Array column integrator
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (zh) 2010-07-03 2016-03-21 生命技術公司 具有微摻雜汲極之化學感測器
US8986930B2 (en) 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
WO2012021733A2 (en) 2010-08-12 2012-02-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US8715932B2 (en) 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US8906612B2 (en) 2010-08-25 2014-12-09 Pacific Biosciences Of California, Inc. Scaffold-based polymerase enzyme substrates
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
US8796036B2 (en) 2010-09-24 2014-08-05 Life Technologies Corporation Method and system for delta double sampling
WO2012054784A1 (en) 2010-10-20 2012-04-26 Li-Cor, Inc. Fluorescent imaging with substituted cyanine dyes
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
CA3060724A1 (en) 2010-11-05 2012-05-10 Genalyte, Inc. Optical analyte detection systems and methods of use
WO2012088341A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
EP2678444B1 (de) 2011-02-23 2016-02-17 Eve Biomedical, Inc. Systeme zur rotationsabhängige transkriptionelle sequenzierung und anwendungsverfahren dafür
EP2689028B1 (de) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolierung von polymerase-nukleinsäurekomplexen und deren ladung auf substrate
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
EP3150750B1 (de) 2011-04-08 2018-12-26 Prognosys Biosciences, Inc. Peptidkonstrukte und testsysteme dafür
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
KR102048274B1 (ko) 2011-09-13 2019-11-25 애질런트 테크놀로지스, 인크. 핵산 시퀀싱을 위하여 5-메톡시, 3'-oh 차단안된, 신속하게 광절단가능한 종료 뉴클레오티드 및 핵산 시퀀싱 방법
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US9322798B2 (en) 2011-12-15 2016-04-26 Intel Corporation Diamond electrode nanogap transducers
WO2013096692A1 (en) * 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
KR101754859B1 (ko) 2011-12-28 2017-07-06 인텔 코포레이션 선택적 표면 고정화 부위를 갖는 나노갭 트랜스듀서
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
EP3222627B1 (de) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymeraseenzymsubstrate mit proteinabschirmung
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
DE102012102983A1 (de) * 2012-04-05 2013-10-10 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen eines kritischen Winkels eines Anregungslichtstrahls
WO2013163207A1 (en) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification of 5-methyl-c in nucleic acid templates
US10544454B2 (en) 2012-05-02 2020-01-28 Ibis Biosciences, Inc. DNA sequencing
ES2833524T3 (es) 2012-05-02 2021-06-15 Ibis Biosciences Inc Secuenciación de ADN
US10458915B2 (en) 2012-05-18 2019-10-29 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
GB2517875A (en) 2012-06-08 2015-03-04 Pacific Biosciences California Modified base detection with nanopore sequencing
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
WO2013192106A1 (en) 2012-06-17 2013-12-27 Pacific Biosciences Of California, Inc. Filter architecture for analytical devices
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
WO2014130686A2 (en) 2013-02-20 2014-08-28 Eve Biomedical, Inc. Methods and compositions for nanostructure-based nucleic acid sequencing
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP2972279B1 (de) 2013-03-15 2021-10-06 Life Technologies Corporation Chemischer sensor mit stetigen sensoroberflächen
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014143637A1 (en) 2013-03-15 2014-09-18 The Board Of Trustees Of The University Of Illinois Methods and compositions for enhancing immunoassays
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
CN108864232B (zh) 2013-03-15 2022-01-04 伊鲁米纳剑桥有限公司 修饰的核苷或核苷酸
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
WO2014210223A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10190143B2 (en) 2013-07-08 2019-01-29 The Board Of Regents Of The University Of Texas System Method for synthesizing selectively labeled RNA
US9957291B2 (en) 2013-08-05 2018-05-01 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
ES2739430T3 (es) 2013-08-19 2020-01-31 Singular Bio Inc Ensayos para la detección de una sola molécula y uso de los mismos
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
CN105723222B (zh) 2013-10-23 2019-01-22 吉尼亚科技公司 使用纳米孔的高速分子感测
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
CN105917003A (zh) 2013-11-17 2016-08-31 宽腾矽公司 用于生物和化学试样的快速分析的有源像素集成装置
CN114471756B (zh) 2013-11-18 2024-04-16 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
WO2015128157A1 (en) 2014-02-25 2015-09-03 Roche Diagnostics Gmbh Method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof
CA2943952A1 (en) 2014-03-24 2015-10-01 The Trustees Of Columbia University In The City Of New York Chemical methods for producing tagged nucleotides
WO2015179098A1 (en) 2014-05-21 2015-11-26 Integenx Inc. Fluidic cartridge with valve mechanism
GB201414098D0 (en) * 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
AU2015300778B2 (en) 2014-08-08 2021-02-25 Quantum-Si Incorporated Optical system and assay chip for probing, detecting, and analyzing molecules
EP3471402B1 (de) 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrierte vorrichtung zur temporären gruppierung von empfangenen photonen
MX2017001808A (es) 2014-08-08 2018-02-08 Quantum Si Inc Dispositivo integrado con fuente de luz externa para el sondeo, deteccion y analisis de moleculas.
CN113092563A (zh) 2014-10-22 2021-07-09 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
WO2016065248A1 (en) 2014-10-23 2016-04-28 Complete Genomics, Inc. Signal confinement sequencing (scs) and nucleotide analogues for signal confinement sequencing
US11218410B2 (en) * 2014-11-10 2022-01-04 Marvell Asia Pte, Ltd. Hybrid wildcard match table
US11943142B2 (en) * 2014-11-10 2024-03-26 Marvell Asia Pte, LTD Hybrid wildcard match table
US10344325B2 (en) 2014-11-11 2019-07-09 Life Technologies Corporation Thiolated nucleotide analogues for nucleic acid synthesis
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
WO2016126941A1 (en) 2015-02-04 2016-08-11 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
CN107532207B (zh) 2015-04-10 2021-05-07 空间转录公司 生物样本的空间区别、多重核酸分析
MY181693A (en) 2015-04-15 2021-01-01 Biosearch Tech Inc Dual quencher probes
CN106153806B (zh) * 2015-05-11 2018-01-19 上海市计量测试技术研究院 三磷酸腺苷的纯度检测方法
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
WO2017024017A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
EP3350350B1 (de) * 2015-11-19 2021-04-14 Pacific Biosciences of California, Inc. Verbindungen und systeme zur verbesserung der signaldetektion
WO2017084580A1 (en) 2015-11-19 2017-05-26 Peking University Methods for obtaining and correcting biological sequence information
CN106755292B (zh) * 2015-11-19 2019-06-18 赛纳生物科技(北京)有限公司 一种磷酸修饰荧光团的核苷酸分子测序方法
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
CN113321943A (zh) 2015-11-20 2021-08-31 加利福尼亚太平洋生物科学股份有限公司 标记的核苷酸类似物、反应混合物以及测序方法和系统
EP3376996B1 (de) 2015-11-20 2022-09-07 Pacific Biosciences of California, Inc. Geschützte farbstoffmarkierte reagenzien
TW202139919A (zh) 2016-02-17 2021-11-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
WO2018052877A1 (en) * 2016-09-16 2018-03-22 The Board Of Regents Of The University Of Texas System Redox activatable fluorescent sensors
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
JP2020501573A (ja) 2016-12-19 2020-01-23 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ シークエンシング反応用の重合酵素
TWI796311B (zh) 2016-12-22 2023-03-21 美商寬騰矽公司 具有直接合併像素之整合式光電偵測器
KR20200004849A (ko) 2017-05-05 2020-01-14 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
US11001885B2 (en) 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
EP3658682A4 (de) 2017-07-24 2021-06-02 Quantum-si Incorporated Markierte reaktantzusammensetzungen mit hoher intensität und verfahren zur sequenzierung
WO2019067635A1 (en) * 2017-09-27 2019-04-04 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
AU2019288394A1 (en) 2018-06-22 2021-01-07 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CA3117889A1 (en) 2018-11-15 2020-05-22 Quantum-Si Incorporated Methods and compositions for protein sequencing
KR20210098491A (ko) * 2018-11-30 2021-08-10 진인포섹, 아이엔씨. 무작위 올리고뉴클레오티드를 생성하고 그 서열을 결정하는 방법
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
BR112021013787A2 (pt) 2019-01-23 2021-10-05 Quantum-Si Incorporated Composições reagentes marcadas com alta densidade e métodos para sequenciamento
CN113710686A (zh) 2019-02-19 2021-11-26 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
CN114302961A (zh) 2019-06-28 2022-04-08 宽腾矽公司 用于测序反应的聚合酶
CN110609020B (zh) * 2019-08-15 2021-10-01 济南大学 基于回文分子信标检测atp的生物传感器及其制备方法和应用
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
EP4090667A1 (de) 2020-01-21 2022-11-23 Quantum-si Incorporated Verbindungen und verfahren zur selektiven c-terminalen markierung
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4110956A1 (de) 2020-02-28 2023-01-04 CureVac Netherlands B.V. Hidden-frame-neoantigene
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4242325A3 (de) 2020-04-22 2023-10-04 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054A1 (de) 2020-06-02 2023-04-05 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220162680A1 (en) * 2020-11-20 2022-05-26 Illumina, Inc. Compositions and methods for sequencing using fluorophores and quenchers or donors
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN112552363B (zh) * 2020-12-24 2022-03-18 山东大学 一种偶联抗坏血酸的核苷酸探针及其制备方法和应用
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
WO2023014363A1 (en) * 2021-08-05 2023-02-09 Hewlett-Packard Development Company, L.P. Polymerase chain reaction test well including magnetic portion
EP4355901A1 (de) * 2021-08-05 2024-04-24 Hewlett-Packard Development Company, L.P. Testbohrloch mit boden mit einer einzigen öffnung
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023068931A1 (en) 2021-10-21 2023-04-27 Curevac Netherlands B.V. Cancer neoantigens
WO2023076772A1 (en) * 2021-11-01 2023-05-04 New England Biolabs, Inc. Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes
EP4174491A1 (de) * 2021-11-01 2023-05-03 New England Biolabs, Inc. Zusammensetzungen und verfahren zum detektieren von pyrophosphatprodukten aus enzymreaktionen mit pyridylazoanilinfarbstoffen
CN114317684B (zh) * 2021-12-15 2023-12-26 南京大学 一种基于tna分子的细胞内镁离子成像的方法

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4318981A (en) * 1980-04-24 1982-03-09 Miles Laboratories, Inc. Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5013831A (en) 1984-01-30 1991-05-07 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5849478A (en) 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US5273879A (en) 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
JP2955759B2 (ja) * 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450069A4 (en) 1989-10-25 1993-03-17 Nkk Corporation Method of continuously plating metal plates
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
RU1794088C (ru) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US6100099A (en) * 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210168D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5489508A (en) * 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
IL108497A0 (en) 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
ES2176308T3 (es) * 1993-10-28 2002-12-01 Houston Advanced Res Ct Dispositivo de microestructura porosa que permite un flujo.
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2182517C (en) * 1994-02-07 2001-08-21 Theo Nikiforov Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5635608A (en) 1994-11-08 1997-06-03 Molecular Probes, Inc. α-carboxy caged compounds
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5736318A (en) * 1995-03-17 1998-04-07 President And Fellows Of Harvard College Method and kit for evaluating human papillomavirus transformed cells
GB9509336D0 (en) 1995-05-09 1995-06-28 Dynal As Chemical method
US5872243A (en) 1995-06-30 1999-02-16 Molecular Probes, Inc. Caged nucleotides
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
JP3439221B2 (ja) * 1995-12-18 2003-08-25 ワシントン ユニヴァーシティ 蛍光共鳴エネルギー移動を利用する核酸分析方法
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US6099823A (en) * 1996-02-16 2000-08-08 Millennium Pharmaceuticals, Inc. Compositions and methods for the treatment and diagnosis of cardiovascular disease
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5846737A (en) 1996-07-26 1998-12-08 Molecular Probes, Inc. Conjugates of sulforhodamine fluorophores with enhanced fluorescence
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
US5942397A (en) * 1996-12-11 1999-08-24 Tarlov; Michael J. Surface immobilization of biopolymers
GB9626815D0 (en) † 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
US5842767A (en) * 1997-03-11 1998-12-01 Farlight Corporation Highly efficient illuminator and method of use thereof
DE69824716D1 (de) * 1997-04-01 2004-07-29 Manteia S A Methode zur sequenzierung von nukleinsäuren
WO1998049344A1 (en) * 1997-04-28 1998-11-05 Lockheed Martin Energy Research Corporation Method and apparatus for analyzing nucleic acids
EP0967219A4 (de) * 1997-07-11 2002-09-11 Rikagaku Kenkyusho Verbindungen mit energieübertragungsfunktion und eine methode zur dna-basensequenzierung unter verwendung dieser verbindungen
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
EP2267165B1 (de) * 1997-07-28 2016-11-30 Gen-Probe Incorporated Sequenzanalyse von Nukleinsäuren
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999057321A1 (en) * 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6287821B1 (en) * 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
AU772281B2 (en) * 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
EP1368460B1 (de) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Sequenzbestimmung in echtzeit
WO2004092331A2 (en) * 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7767400B2 (en) 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis

Also Published As

Publication number Publication date
DE69930310T3 (de) 2009-12-17
US20150218632A1 (en) 2015-08-06
US9845501B2 (en) 2017-12-19
CA2355816C (en) 2007-10-30
US7229799B2 (en) 2007-06-12
US20090082212A1 (en) 2009-03-26
WO2000036152A1 (en) 2000-06-22
JP4638043B2 (ja) 2011-02-23
ATE319857T1 (de) 2006-03-15
US8980584B2 (en) 2015-03-17
AU2180200A (en) 2000-07-03
DE69930310T2 (de) 2006-12-28
JP2010142250A (ja) 2010-07-01
JP2002532104A (ja) 2002-10-02
WO2000036151A1 (en) 2000-06-22
AU772281B2 (en) 2004-04-22
US20010018184A1 (en) 2001-08-30
US20030194740A1 (en) 2003-10-16
EP1141409B1 (de) 2006-03-08
EP1141409A4 (de) 2003-02-26
US20020115076A1 (en) 2002-08-22
WO2000036151A9 (en) 2001-08-02
CA2355816A1 (en) 2000-06-22
US20100093068A1 (en) 2010-04-15
US6232075B1 (en) 2001-05-15
US20080241833A1 (en) 2008-10-02
US6762048B2 (en) 2004-07-13
US6255083B1 (en) 2001-07-03
AU2360200A (en) 2000-07-03
US6306607B2 (en) 2001-10-23
EP1141409B2 (de) 2009-05-27
EP1141409A1 (de) 2001-10-10
US8192961B2 (en) 2012-06-05
US8530154B2 (en) 2013-09-10
US20090170074A1 (en) 2009-07-02

Similar Documents

Publication Publication Date Title
ATE319857T1 (de) Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DE69937223D1 (de) Verfahren zur synthese von nukleinsäuren
DE60128720D1 (de) Mikroarray-verfahren zur genotypisierung mehrerer proben für mehrere loci
ATE397092T1 (de) Methode zur direkten sequenzierung von nukleinsäuren
NO20072722L (no) Preparater og fremgangsmate for a rense nukleinsyrer fra stabiliseringsreagenser
DE50015677D1 (de) Methode zur isolierung von dna aus biologischen materialien
US20080108804A1 (en) Method for modifying RNAS and preparing DNAS from RNAS
ATE356866T1 (de) Polymerasenchimären
DE69225074D1 (de) Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen
ATE240408T1 (de) Verfahren und verbindungen zur analysierung von nukleinsaeuren durch massenspektrometrie
DE69016826T2 (de) Verfahren zur Synthese von Ribonukleinsäure (RNS).
CN108611398A (zh) 通过新一代测序进行基因分型
ATE106948T1 (de) Verfahren zur amplifizierung von nukleinsäuren.
ATE356222T1 (de) Massives parallelverfahren zur dekodierung von dna und rna
ATE443136T1 (de) Methode für dna synthese
ATE376067T1 (de) Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
ATE474065T1 (de) Screeningverfahren zur identifizierung von pufa- pks in proben
HUP0302593A2 (hu) Önállóan replikálódó RNS molekula hepatitis C vírusból
CN104630211A (zh) 一种Small RNA cDNA文库的构建方法
DE69842183D1 (de) Hochempfindliche polymerasen und ihre verwendungen
WO2002044414A3 (en) Purification of dna sequencing reactions using silica magnetic particles
FR2568588B1 (fr) Sonde et procede pour la detection de micro-organismes determines, notamment des legionella dans les milieux les contenant
ATE264865T1 (de) Verfahren zum nachweis von biologischen materialen mit boviner herkunft, und oligonukleotiden die es gebraucht
ATE420975T1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren

Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. (N. D., US

8328 Change in the person/name/address of the agent

Representative=s name: VOSSIUS & PARTNER, 81675 MUENCHEN

8366 Restricted maintained after opposition proceedings