HRP20231442T1 - Medij za kulturu matičnih stanica - Google Patents

Medij za kulturu matičnih stanica Download PDF

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HRP20231442T1
HRP20231442T1 HRP20231442TT HRP20231442T HRP20231442T1 HR P20231442 T1 HRP20231442 T1 HR P20231442T1 HR P20231442T T HRP20231442T T HR P20231442TT HR P20231442 T HRP20231442 T HR P20231442T HR P20231442 T1 HRP20231442 T1 HR P20231442T1
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culture medium
population
epithelial stem
cells
adult epithelial
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HRP20231442TT
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Johannes Carolus Clevers
Toshiro Sato
Meritxell Huch Ortega
Wouter Richard Karthaus
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Koninklijke Nederlandse Akademie Van Wetenschappen (Knaw)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44511945&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HRP20231442(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US13/194,866 external-priority patent/US9752124B2/en
Application filed by Koninklijke Nederlandse Akademie Van Wetenschappen (Knaw) filed Critical Koninklijke Nederlandse Akademie Van Wetenschappen (Knaw)
Publication of HRP20231442T1 publication Critical patent/HRP20231442T1/hr

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Claims (16)

1. Medij za kulturu za ekspandiranje populacije odraslih epitelnih matičnih stanica, naznačen time, da navedeni medij za kulturu sadržava: i. bilo koji Rspondin 1-4; i ii. jedan ili više TGF-beta inhibitora, pri čemu inhibitor je TGF-beta inhibitor ako on može inhibirati TGF-beta signaliziranje u staničnom testu gdje se stanice stabilno transfektiraju s reporterskim konstruktom koji sadrži ljudski promotor PAI-1.
2. Medij za kulturu za ekspandiranje populacije odraslih epitelnih matičnih stanica, naznačen time, da navedeni medij za kulturu sadržava: i. agonist od Lgr5; ii. jedan ili više TGF-beta inhibitora, pri čemu inhibitor je TGF-beta inhibitor ako on može inhibirati TGF-beta signaliziranje u staničnom testu gdje se stanice čvrsto transfektiraju s reporterskim konstruktom koji sadrži ljudski promotor PAI-1.
3. Medij za kulturu prema patentnom zahtjevu 1 ili zahtjevu 2, naznačen time, da se jedan ili više TGF-beta inhibitora veže na ALK5, ALK4 i/ili ALK7, i pritom smanjuje njihovu aktivnost.
4. Medij za kulturu prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, da se jedan ili više TGF-beta inhibitora bira iz skupine koju čine A83-01, SB-431542, SB-505124, SB-525334, SD-208, LY-36494 i SJN-2511.
5. Medij za kulturu prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, da medij za kulturu sadrži jedan ili više dodatnih sastojaka koji se biraju od sljedećih: Wnt agonist, ligand receptorske tirozin kinaze, nikotinamid, p38 inhibitor, ROCK inhibitor, gastrin, RANKL, GSK3 inhibitor, aktivator signalnog puta prostaglandina i testosteron.
6. Pripravak, naznačen time, da sadržava medij za kulturu prema bilo kojem od patentnih zahtjeva 1 do 5, i izvanstaničnu matricu ili 3D matricu koja oponaša izvanstaničnu matricu putem svojeg međudjelovanja s proteinima stanične membrane kao što su integrini, primjerice izvanstaničnu matricu koja sadrži laminin.
7. Hermetički zabrtvljen spremnik, naznačen time, da sadržava medij za kulturu ili pripravak prema bilo kojem od prethodnih patentnih zahtjeva.
8. Uporaba medija za kulturu prema bilo kojem od patentnih zahtjeva 1 do 5, naznačena time, da je za ekspandiranje odraslih epitelnih matičnih stanica, populacije odraslih epitelnih matičnih stanica, fragmenta tkiva ili organoida.
9. Postupak za ekspandiranje pojedinačne odrasle epitelne matične stanice, populacije odraslih epitelnih matičnih stanica, ili fragmenta tkiva, poželjno u svrhu dobivanja organoida, naznačen time, da postupak obuhvaća uzgoj pojedinačne odrasle epitelne matične stanice ili populacije odraslih epitelnih matičnih stanica u mediju za kulturu, u skladu s bilo kojim od patentnih zahtjeva 1 do 5.
10. Postupak prema patentnom zahtjevu 9, naznačen time, da postupak obuhvaća dovođenje odrasle epitelne matične stanice, populacije odraslih epitelnih matičnih stanica, ili izoliranog fragmenta tkiva i medija za kulturu u doticaj s izvanstaničnom matricom ili 3D matricom koja oponaša izvanstaničnu matricu putem svojeg međudjelovanja s proteinima stanične membrane kao što su integrini, primjerice izvanstaničnu matricu koja sadrži laminin.
11. Postupak prema patentnom zahtjevu 9 ili zahtjevu 10, naznačen time, da obuhvaća: uzgoj odrasle epitelne matične stanice, populacije odraslih epitelnih matičnih stanica, ili fragmenata tkiva u prvom mediju za ekspanziju u skladu s bilo kojim od patentnih zahtjeva 1 do 5; i nastavljanje uzgajanja odrasle epitelne matične stanice, populacije odraslih epitelnih matičnih stanica, ili fragmenata tkiva te nadopunjavanje medija s diferencijacijskim medijem, pri čemu diferencijacijski medij ne sadržava jedan ili više, poželjno nijedan od faktora koji se biraju od sljedećih: TGF-beta inhibitor, p38 inhibitor, nikotinamid i Wnt.
12. Organoid ili populacija stanica, naznačeni time, da se mogu dobiti putem postupka prema bilo kojem od patentnih zahtjeva 9 do 11.
13. Pripravak, naznačen time, da sadrži: i) jedan ili više organoida ili populacija stanica u skladu s patentnim zahtjevom 12; i ii) medij za kulturu u skladu s bilo kojim od patentnih zahtjeva 1 do 5 i/ili izvanstaničnu matricu.
14. Uporaba organoida ex vivo prema patentnom zahtjevu 12, ili populacije stanica prema patentnom zahtjevu 12, ili pripravka prema patentnom zahtjevu 6 ili zahtjevu 13, naznačena time, da je za skrining lijeka, validaciju cilja, otkrivanje cilja, toksikologiju, toksikološke testove, dijagnostiku modela stanice/organa, primjerice za uporabu kao modela bolesti.
15. Organoid prema patentnom zahtjevu 12, ili populacija stanica prema patentnom zahtjevu 12, ili pripravak prema patentnom zahtjevu 6 ili zahtjevu 13, naznačeni time, da su za uporabu u medicini, opcionalno pritom medicina je personalizirana medicina ili regenerativna medicina.
16. Postupak za skrining za terapijski ili profilaktički lijek ili kozmetičko sredstvo, naznačen time, da postupak obuhvaća: uzgoj organoida ili populacije stanica u skladu s patentnim zahtjevom 12, primjerice s medijem za kulturu prema patentnim zahtjevima od 1 do 5; izlaganje navedenog organoida ili navedene populacije stanica jednoj ili biblioteci kandidatskih molekula; procjenjivanje navedenog organoida ili populacije stanica za bilo koje pojave/učinke, primjerice bilo kakvu promjenu u stanici, kao što je smanjivanje ili gubitak proliferacije, morfološka promjena i/ili smrt stanice; i identificiranje kandidatske molekule koja uzrokuje spomenute učinke, kao potencijalnog lijeka ili kozmetičkog sredstva.
HRP20231442TT 2011-06-10 2012-06-11 Medij za kulturu matičnih stanica HRP20231442T1 (hr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161520569P 2011-06-10 2011-06-10
US201161571663P 2011-06-30 2011-06-30
GBGB1111244.8A GB201111244D0 (en) 2011-06-30 2011-06-30 Culture media for stem cells
US201161513461P 2011-07-29 2011-07-29
US13/194,866 US9752124B2 (en) 2009-02-03 2011-07-29 Culture medium for epithelial stem cells and organoids comprising the stem cells
US201261594295P 2012-02-02 2012-02-02
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