ES2926021T3 - Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma - Google Patents

Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma Download PDF

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ES2926021T3
ES2926021T3 ES20164644T ES20164644T ES2926021T3 ES 2926021 T3 ES2926021 T3 ES 2926021T3 ES 20164644 T ES20164644 T ES 20164644T ES 20164644 T ES20164644 T ES 20164644T ES 2926021 T3 ES2926021 T3 ES 2926021T3
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cas9
rgen
guide rna
target
cas9 protein
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Jin-Soo Kim
Seung Woo Cho
Sojung Kim
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Toolgen Inc
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Toolgen Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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ES20164644T 2012-10-23 2013-10-23 Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma Active ES2926021T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261717324P 2012-10-23 2012-10-23
US201361803599P 2013-03-20 2013-03-20
US201361837481P 2013-06-20 2013-06-20

Publications (1)

Publication Number Publication Date
ES2926021T3 true ES2926021T3 (es) 2022-10-21

Family

ID=50544909

Family Applications (4)

Application Number Title Priority Date Filing Date
ES20164644T Active ES2926021T3 (es) 2012-10-23 2013-10-23 Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma
ES24158339T Active ES3005455T3 (en) 2012-10-23 2013-10-23 Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
ES17206792T Active ES2886448T3 (es) 2012-10-23 2013-10-23 Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína CAS o proteína CAS, y uso de los mismos
ES13849670T Active ES2690386T3 (es) 2012-10-23 2013-10-23 Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES24158339T Active ES3005455T3 (en) 2012-10-23 2013-10-23 Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
ES17206792T Active ES2886448T3 (es) 2012-10-23 2013-10-23 Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína CAS o proteína CAS, y uso de los mismos
ES13849670T Active ES2690386T3 (es) 2012-10-23 2013-10-23 Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos

Country Status (16)

Country Link
US (14) US20150284727A1 (cg-RX-API-DMAC7.html)
EP (12) EP3346003B1 (cg-RX-API-DMAC7.html)
JP (10) JP6517143B2 (cg-RX-API-DMAC7.html)
KR (5) KR101656237B1 (cg-RX-API-DMAC7.html)
CN (9) CN110643600A (cg-RX-API-DMAC7.html)
AU (6) AU2013335451C1 (cg-RX-API-DMAC7.html)
BR (1) BR112015008708A2 (cg-RX-API-DMAC7.html)
CA (2) CA3233048A1 (cg-RX-API-DMAC7.html)
DE (2) DE202013012597U1 (cg-RX-API-DMAC7.html)
DK (2) DK4357457T3 (cg-RX-API-DMAC7.html)
ES (4) ES2926021T3 (cg-RX-API-DMAC7.html)
HK (1) HK1257302A1 (cg-RX-API-DMAC7.html)
PL (2) PL4357457T3 (cg-RX-API-DMAC7.html)
PT (1) PT2912175T (cg-RX-API-DMAC7.html)
SG (3) SG10201809566SA (cg-RX-API-DMAC7.html)
WO (1) WO2014065596A1 (cg-RX-API-DMAC7.html)

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