JP6656733B2 - 新規合成した遺伝子ライブラリ - Google Patents

新規合成した遺伝子ライブラリ Download PDF

Info

Publication number
JP6656733B2
JP6656733B2 JP2016533384A JP2016533384A JP6656733B2 JP 6656733 B2 JP6656733 B2 JP 6656733B2 JP 2016533384 A JP2016533384 A JP 2016533384A JP 2016533384 A JP2016533384 A JP 2016533384A JP 6656733 B2 JP6656733 B2 JP 6656733B2
Authority
JP
Japan
Prior art keywords
substrate
genes
locations
less
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016533384A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016527313A5 (enExample
JP2016527313A (ja
Inventor
バニャイ，ウイリアム
ペック，ビル，ジェイムス
フェルナンデス，アンドレス
チェン，シユアン
インデアミューレ，ピエール
Original Assignee
ツイスト バイオサイエンス コーポレーション
ツイスト バイオサイエンス コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52428204&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP6656733(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ツイスト バイオサイエンス コーポレーション, ツイスト バイオサイエンス コーポレーション filed Critical ツイスト バイオサイエンス コーポレーション
Publication of JP2016527313A publication Critical patent/JP2016527313A/ja
Publication of JP2016527313A5 publication Critical patent/JP2016527313A5/ja
Application granted granted Critical
Publication of JP6656733B2 publication Critical patent/JP6656733B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Structural Engineering (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2016533384A 2013-08-05 2014-08-05 新規合成した遺伝子ライブラリ Active JP6656733B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361862457P 2013-08-05 2013-08-05
US201361862445P 2013-08-05 2013-08-05
US61/862,457 2013-08-05
US61/862,445 2013-08-05
PCT/US2014/049834 WO2015021080A2 (en) 2013-08-05 2014-08-05 De novo synthesized gene libraries

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019163456A Division JP2020022453A (ja) 2013-08-05 2019-09-06 新規合成した遺伝子ライブラリ

Publications (3)

Publication Number Publication Date
JP2016527313A JP2016527313A (ja) 2016-09-08
JP2016527313A5 JP2016527313A5 (enExample) 2017-09-07
JP6656733B2 true JP6656733B2 (ja) 2020-03-04

Family

ID=52428204

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2016533384A Active JP6656733B2 (ja) 2013-08-05 2014-08-05 新規合成した遺伝子ライブラリ
JP2019163456A Pending JP2020022453A (ja) 2013-08-05 2019-09-06 新規合成した遺伝子ライブラリ
JP2021057779A Pending JP2021118690A (ja) 2013-08-05 2021-03-30 新規合成した遺伝子ライブラリ
JP2023058883A Pending JP2023093506A (ja) 2013-08-05 2023-03-31 新規合成した遺伝子ライブラリ

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2019163456A Pending JP2020022453A (ja) 2013-08-05 2019-09-06 新規合成した遺伝子ライブラリ
JP2021057779A Pending JP2021118690A (ja) 2013-08-05 2021-03-30 新規合成した遺伝子ライブラリ
JP2023058883A Pending JP2023093506A (ja) 2013-08-05 2023-03-31 新規合成した遺伝子ライブラリ

Country Status (14)

Country Link
US (20) US9409139B2 (enExample)
EP (4) EP3722442B1 (enExample)
JP (4) JP6656733B2 (enExample)
KR (6) KR102351838B1 (enExample)
CN (2) CN111593414A (enExample)
CA (1) CA2918258A1 (enExample)
DK (1) DK3030682T3 (enExample)
EA (1) EA034459B1 (enExample)
ES (2) ES2943498T3 (enExample)
GB (1) GB2533173A (enExample)
PL (1) PL3030682T3 (enExample)
SG (1) SG11201600853UA (enExample)
TW (5) TWI695067B (enExample)
WO (1) WO2015021080A2 (enExample)

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3038300T3 (en) 2010-12-30 2025-10-10 Found Medicine Inc Optimization of multigene analysis of tumor samples
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
AU2014308980C1 (en) 2013-08-19 2021-09-30 Invitae Corporation Assays for single molecule detection and use thereof
NZ730044A (en) * 2014-09-15 2024-08-30 Abvitro Llc High-throughput nucleotide library sequencing
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
AU2015357573B2 (en) 2014-12-05 2022-04-07 Foundation Medicine, Inc. Multigene analysis of tumor samples
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
AU2016219943A1 (en) * 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20190057445A (ko) * 2015-09-14 2019-05-28 에센릭스 코프. 샘플 특히 혈액샘플을 분석하기 위한 장치와 시스템 및 그 사용 방법
EA201890763A1 (ru) * 2015-09-18 2018-08-31 Твист Байосайенс Корпорейшн Библиотеки вариантных олигонуклеиновых кислот и их синтез
KR102794025B1 (ko) * 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN108521782A (zh) * 2015-10-21 2018-09-11 豪夫迈·罗氏有限公司 含氟聚合物作为疏水层以支持用于纳米孔的脂质双层形成的用途
WO2017095958A1 (en) * 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3386550B1 (en) 2015-12-07 2021-01-20 Arc Bio, LLC Methods for the making and using of guide nucleic acids
AU2017240164A1 (en) * 2016-04-01 2018-11-15 HealthTell, Inc. Array-based peptide libraries for therapeutic antibody characterization
WO2017214574A1 (en) * 2016-06-10 2017-12-14 Twist Bioscience Corporation Systems and methods for automated annotation and screening of biological sequences
WO2017218293A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
CN110088281A (zh) * 2016-08-03 2019-08-02 特韦斯特生物科学公司 用于多核苷酸合成的纹理化表面
WO2018038772A1 (en) * 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP3516528B1 (en) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nucleic acid based data storage
AU2017359450A1 (en) 2016-11-09 2019-05-30 HealthTell, Inc. Coatings with tunable amine density
CN110072870A (zh) 2016-11-09 2019-07-30 健康之语公司 预组装的、受保护的、化学稳定的化学选择性接头
KR102569164B1 (ko) * 2016-11-18 2023-08-21 트위스트 바이오사이언스 코포레이션 조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
NZ754331A (en) 2016-12-22 2025-09-26 Illumina Inc Flow cell package and method for making the same
JP2020508661A (ja) 2017-02-22 2020-03-26 ツイスト バイオサイエンス コーポレーション 核酸ベースのデータ保存
WO2018170164A1 (en) * 2017-03-15 2018-09-20 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
US11795580B2 (en) 2017-05-02 2023-10-24 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
US12227775B2 (en) 2017-05-22 2025-02-18 The Charles Stark Draper Laboratory, Inc. Modified template-independent DNA polymerase
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
JP7282692B2 (ja) * 2017-06-07 2023-05-29 アーク バイオ， エルエルシー ガイド核酸の作製および使用
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11201912057RA (en) * 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US20190076814A1 (en) 2017-09-11 2019-03-14 Synthego Corporation Biopolymer synthesis system and method
EP3681637A4 (en) * 2017-09-11 2021-06-02 Synthego Corporation SYSTEM AND PROCEDURE FOR BIOPOLYMER SYNTHESIS
CN111295444B (zh) * 2017-09-25 2024-08-09 哈斯达克科学公司 用于寡核苷酸指导的组合化学的多项式编码
JP7361700B2 (ja) * 2017-09-25 2023-10-16 プレキシウム インコーポレイテッド オリゴヌクレオチドにコードされた化学ライブラリ
US11402400B2 (en) 2017-10-13 2022-08-02 Hewlett-Packard Development Company, L.P. Partition liquid into samples
KR102637566B1 (ko) * 2017-10-20 2024-02-16 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
CN112041438B (zh) 2018-01-04 2025-05-23 特韦斯特生物科学公司 基于dna的数字信息存储
JP7262481B2 (ja) * 2018-04-13 2023-04-21 エフ． ホフマン－ラ ロシュ アーゲー 分析物の検出および分析のための方法および組成物
US12319712B2 (en) 2018-05-09 2025-06-03 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photoactivated agents
WO2019222612A1 (en) * 2018-05-17 2019-11-21 Georgia Tech Research Corporation Devices, systems, and methods of electronic modulation of polymerase for dna synthesis
WO2019222650A1 (en) * 2018-05-17 2019-11-21 The Charles Stark Draper Laboratory, Inc. Apparatus for high density information storage in molecular chains
CN118957038A (zh) 2018-05-18 2024-11-15 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
CA3102460A1 (en) 2018-06-11 2019-12-19 Foundation Medicine, Inc. Compositions and methods for evaluating genomic alterations
US11854669B1 (en) 2018-07-30 2023-12-26 National Technology & Engineering Solutions Of Sandia, Llc Synthetic nucleic acids for information storage and transmission
CN109136331A (zh) * 2018-08-22 2019-01-04 中山大学 一种高灵敏度高特异性错配核酸序列的设计方法
JP7322365B2 (ja) * 2018-09-06 2023-08-08 株式会社レゾナック サセプタ及び化学気相成長装置
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
WO2020102583A1 (en) * 2018-11-14 2020-05-22 Synthomics, Inc. Methods, devices, and systems for biopolymer synthesis
CA3120107A1 (en) * 2018-11-30 2020-06-04 Geneinfosec, Inc. A method for generating random oligonucleotides and determining their sequence
EP3894589A2 (en) * 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays using deconvolution
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
TWI845600B (zh) 2019-01-24 2024-06-21 美商普羅米修斯生物科學股份有限公司 Gpr35調節劑
CA3125496A1 (en) * 2019-01-28 2020-08-06 Bgi Shenzhen Sequencing chip and manufacturing mehtod therefor
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
KR102187795B1 (ko) * 2019-04-30 2020-12-08 (주)디엑솜 디옥시우리딘을 이용한 차세대 염기서열 분석을 위한 라이브러리 제조 방법
US11229909B2 (en) * 2019-05-31 2022-01-25 Illumina, Inc. Flow cell with selective deposition or activation of nucleotides
WO2020257612A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN110349628B (zh) * 2019-06-27 2021-06-15 广东药科大学 一种蛋白质磷酸化位点识别方法、系统、装置及存储介质
CN114555822A (zh) * 2019-08-22 2022-05-27 新英格兰生物实验室公司 具有修饰核苷酸的单链dna的切割
CN110531099B (zh) * 2019-08-23 2024-04-12 湖南莱博赛医用机器人有限公司 一种分体式玻片盒及细胞分析设备
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
WO2021061829A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
CZ2019720A3 (cs) * 2019-11-23 2021-01-13 Grade Medical s.r.o. Zařízení pro přípravu funkcionalizovaných substrátů
CN111072729B (zh) * 2019-12-13 2023-06-13 天津大学 一种寡核苷酸合成用固相载体装置及其选择性修饰方法
CN115243787A (zh) * 2019-12-30 2022-10-25 源点生物科技股份有限公司 使用酶制备核酸序列的装置及方法
WO2021142133A1 (en) * 2020-01-07 2021-07-15 Elegen Corporation Dna assembly in microfluidics device having integrated solid-phase columns
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
WO2021222316A2 (en) 2020-04-27 2021-11-04 Twist Bioscience Corporation Variant nucleic acid libraries for coronavirus
US12110516B2 (en) 2020-05-12 2024-10-08 Illumina, Inc. Thermostable terminal deoxynucleotidyl transferase
KR20230035237A (ko) 2020-05-12 2023-03-13 일루미나, 인코포레이티드 재조합 말단 데옥시뉴클레오티딜 트랜스퍼라제를 사용하여 변형된 염기를 갖는 핵산의 생성
KR20230024399A (ko) * 2020-06-16 2023-02-20 디엔에이 스크립트 효소적 폴리뉴클레오티드 합성을 위한 시스템, 장치 및 키트
JP2023535287A (ja) 2020-07-21 2023-08-17 イルミナ・シンガポール・プライベイト・リミテッド ＴｄＴに基づく酵素的核酸の基質としての塩基修飾ヌクレオチド
EP3958450A1 (de) * 2020-08-21 2022-02-23 Schneider Electric Industries SAS Linearmotorsystem und betriebsverfahren für ein solches
US20220106586A1 (en) * 2020-08-25 2022-04-07 Twist Bioscience Corporation Compositions and methods for library sequencing
JP2023539245A (ja) 2020-08-26 2023-09-13 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１ｒ変異体に関する方法および組成物
US11970697B2 (en) 2020-10-19 2024-04-30 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
CN116997411A (zh) * 2021-01-13 2023-11-03 加利福尼亚太平洋生物科学股份有限公司 使用胶体组装的表面结构化
CA3206018A1 (en) 2021-01-21 2022-07-28 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
US20250304953A1 (en) * 2021-02-18 2025-10-02 Seoul National University R&Db Foundation Method for purifying nucleic acid library
EP4294567A1 (en) * 2021-02-18 2023-12-27 Battelle Memorial Institute Nanowell array device for high throughput sample analysis
WO2022204102A1 (en) * 2021-03-22 2022-09-29 Genscript Usa Inc. Universal linker reagents for dna synthesis
US12258406B2 (en) 2021-03-24 2025-03-25 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
CN113262730B (zh) * 2021-03-29 2022-11-22 上海迪赢生物科技有限公司 一种基于簇式阵列的高通量自动化基因合成装置
US20220356463A1 (en) * 2021-04-09 2022-11-10 Twist Bioscience Corporation Libraries for mutational analysis
WO2022221853A1 (en) 2021-04-13 2022-10-20 Elegen Corp. Methods and compositions for cell-free cloning
EP4334516A1 (en) * 2021-05-03 2024-03-13 Twist Bioscience Corporation Variant nucleic acid libraries for ion channels
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
EP4147712A1 (en) 2021-09-13 2023-03-15 OncoDNA Method to generate a double-stranded dna pool encoding neoantigens of a tumor of a patient
US20240379186A1 (en) 2021-09-13 2024-11-14 Oncodna Method to generate personalized neoantigens of a tumor of a patient
CN114100714B (zh) * 2021-11-22 2023-01-17 上海睿度光电科技有限公司 一种核酸或多肽高通量合成芯片及其用途
WO2023130123A2 (en) 2022-01-03 2023-07-06 Twist Bioscience Corporation Bispecific sars-cov-2 antibodies and methods of use
WO2023132885A1 (en) * 2022-01-04 2023-07-13 Modernatx, Inc. Methods of purifying dna for gene synthesis
GB202205696D0 (en) * 2022-04-19 2022-06-01 Moligo Tech Ab Method for producing double stranded DNA
GB2621159B (en) * 2022-08-04 2025-08-13 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor
WO2024047178A1 (en) 2022-09-02 2024-03-07 Eleven Therapeutics Ltd A method for simultaneous synthesis of a plurality of oligonucleotides
WO2024073136A1 (en) * 2022-09-30 2024-04-04 Mary Hitchcock Memorial Hospital, For Itself And On Behalf Of Dartmouth-Hitchcock Clinic Rapid reconstruction of large nucleic acids
EP4364752A1 (en) 2022-11-07 2024-05-08 OncoDNA Improved vaccine
WO2024112803A2 (en) * 2022-11-22 2024-05-30 Yale University Methods and kits for microscopic imaging
US12415172B2 (en) 2022-12-22 2025-09-16 Synthego Corporation Systems and method for automated oligonucleotide synthesis
EP4642890A1 (en) * 2022-12-31 2025-11-05 DNA Script Heat treatment means for printing device of an enzymatic synthesis apparatus
CN116434843A (zh) * 2023-03-29 2023-07-14 赛纳生物科技(北京)有限公司 一种碱基测序质量评估方法
WO2024216064A1 (en) 2023-04-14 2024-10-17 Twist Bioscience Corporation Compositions and methods related to dkk1 binders
WO2025072262A1 (en) * 2023-09-26 2025-04-03 Instance, Inc. Ultra-high capacity dna synthesis to power functionally generative biological design

Family Cites Families (935)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1005368A (en) 1909-10-29 1911-10-10 John M Travis Faucet.
US1027241A (en) 1911-07-03 1912-05-21 James Mccutcheon Coleman Dump-car construction.
US3549368A (en) 1968-07-02 1970-12-22 Ibm Process for improving photoresist adhesion
US3920714A (en) 1972-11-16 1975-11-18 Weber Heinrich Process for the production of polymeric hydrocarbons with reactive silyl side groups
GB1550867A (en) 1975-08-04 1979-08-22 Hughes Aircraft Co Positioning method and apparatus for fabricating microcircuit devices
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
EP0090789A1 (en) 1982-03-26 1983-10-05 Monsanto Company Chemical DNA synthesis
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
JPS59224123A (ja) 1983-05-20 1984-12-17 Oki Electric Ind Co Ltd ウエハアライメントマ−ク
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
JPS61141761A (ja) 1984-12-12 1986-06-28 Kanegafuchi Chem Ind Co Ltd 硬化性組成物
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US4613398A (en) 1985-06-06 1986-09-23 International Business Machines Corporation Formation of etch-resistant resists through preferential permeation
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4726877A (en) 1986-01-22 1988-02-23 E. I. Du Pont De Nemours And Company Methods of using photosensitive compositions containing microgels
US4808511A (en) 1987-05-19 1989-02-28 International Business Machines Corporation Vapor phase photoresist silylation process
JPH07113774B2 (ja) 1987-05-29 1995-12-06 株式会社日立製作所 パタ−ンの形成方法
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
DE69028725T2 (de) 1989-02-28 1997-03-13 Canon Kk Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung
US5459039A (en) 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US6008031A (en) 1989-05-12 1999-12-28 Duke University Method of analysis and manipulation of DNA utilizing mismatch repair systems
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US6087482A (en) 1990-07-27 2000-07-11 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
FI87886C (fi) 1990-09-06 1993-03-10 Instrumentarium Oy Faestorgan
DE69119083T2 (de) 1990-09-27 1996-10-17 Invitrogen Corp Direkte klonierung von pcr amplifizierten nukleinsäuren
GB9025236D0 (en) 1990-11-20 1991-01-02 Secr Defence Silicon-on porous-silicon;method of production
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
EP0834575B1 (en) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identification of nucleic acids in samples
AU663300B2 (en) 1990-12-06 1995-10-05 Affymetrix, Inc. Very large scale immobilized polymer synthesis
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5137814A (en) 1991-06-14 1992-08-11 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
US5449754A (en) 1991-08-07 1995-09-12 H & N Instruments, Inc. Generation of combinatorial libraries
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US7045289B2 (en) 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
JP3939338B2 (ja) * 1991-11-22 2007-07-04 アフィメトリックス， インコーポレイテッド ポリマー合成に対する組合わせの戦略
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1993020236A1 (en) 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
JP2553322Y2 (ja) 1992-05-11 1997-11-05 サンデン株式会社 飲料抽出装置のフィルタ送り機構
DK0652973T3 (da) 1992-07-31 1997-09-15 Behringwerke Ag Fremgangsmåde til indføring af definerede sekvenser ved 3-enden af polynukleotider
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
JP3176444B2 (ja) 1992-10-01 2001-06-18 株式会社リコー 水性インク及びこれを用いた記録方法
DE4241045C1 (de) 1992-12-05 1994-05-26 Bosch Gmbh Robert Verfahren zum anisotropen Ätzen von Silicium
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
US5395753A (en) 1993-02-19 1995-03-07 Theratech, Inc. Method for diagnosing rheumatoid arthritis
DE69433010T2 (de) 1993-04-12 2004-06-09 Northwestern University, Evanston Verfahren zur darstellung von oligonukleotiden
US7135312B2 (en) * 1993-04-15 2006-11-14 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5455239A (en) 1993-08-05 1995-10-03 Merck & Co. Inc. 3-aryl of heteroaryl-7-heteroaralkylamido cephalosporin compounds, compositions and methods of use
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
CN1039623C (zh) 1993-10-22 1998-09-02 中国人民解放军军事医学科学院毒物药物研究所 一种防治运动病综合征的药物组合物及其制备方法
US6893816B1 (en) 1993-10-28 2005-05-17 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
EP1157743B1 (en) 1993-10-28 2009-03-11 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6015880A (en) 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
DE69534464T2 (de) 1994-03-29 2006-09-28 Novozymes A/S Alkalische amylase aus bacellus
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
SE512382C2 (sv) 1994-04-26 2000-03-06 Ericsson Telefon Ab L M Anordning och förfarande för att placera långsträckta element mot eller invid en yta
ATE198511T1 (de) 1994-04-29 2001-01-15 Perkin Elmer Corp Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
US6287850B1 (en) 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
AU689924B2 (en) 1994-06-23 1998-04-09 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5530516A (en) 1994-10-04 1996-06-25 Tamarack Scientific Co., Inc. Large-area projection exposure system
US6613560B1 (en) 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
US6635226B1 (en) 1994-10-19 2003-10-21 Agilent Technologies, Inc. Microanalytical device and use thereof for conducting chemical processes
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
JPH11511900A (ja) 1994-11-22 1999-10-12 コンプレツクス フルイツド システムズ，インコーポレーテツド マイクロエレクトロニクス用途のための非アミン系フォトレジスト密着促進剤
US5688642A (en) 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US6446682B1 (en) 1995-06-06 2002-09-10 James P. Viken Auto-loading fluid exchanger and method of use
US5877280A (en) 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US5707806A (en) 1995-06-07 1998-01-13 Genzyme Corporation Direct sequence identification of mutations by cleavage- and ligation-associated mutation-specific sequencing
US5780613A (en) 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
JP2000501615A (ja) 1995-12-15 2000-02-15 アマーシャム・ライフ・サイエンス・インコーポレーテッド 酵素増幅中に生じる変異配列の検出および除去のためのミスマッチ修復系を用いる方法
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
US7432048B2 (en) 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6090606A (en) 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7527928B2 (en) 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6706875B1 (en) 1996-04-17 2004-03-16 Affyemtrix, Inc. Substrate preparation process
US5869245A (en) 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
US5863801A (en) 1996-06-14 1999-01-26 Sarnoff Corporation Automated nucleic acid isolation
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US5853993A (en) 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
AU5173598A (en) 1996-11-08 1998-06-10 Ikonos Corporation Method for coating substrates
US5750672A (en) 1996-11-22 1998-05-12 Barrskogen, Inc. Anhydrous amine cleavage of oligonucleotides
DE69739108D1 (de) 1996-11-29 2008-12-24 Third Wave Tech Inc Fen-1 endonukleasen, gemische und spaltmethoden
CA2389358C (en) * 1996-12-31 2008-07-15 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
ES2241120T3 (es) 1997-02-12 2005-10-16 Invitrogen Corporation Metodos para liofilizar celulas competentes.
US5882496A (en) 1997-02-27 1999-03-16 The Regents Of The University Of California Porous silicon structures with high surface area/specific pore size
US6770748B2 (en) 1997-03-07 2004-08-03 Takeshi Imanishi Bicyclonucleoside and oligonucleotide analogue
EP0970101A2 (en) 1997-03-20 2000-01-12 University Of Washington Solvent for biopolymer synthesis, solvent microdroplets and methods of use
US6028189A (en) 1997-03-20 2000-02-22 University Of Washington Solvent for oligonucleotide synthesis and methods of use
US6419883B1 (en) 1998-01-16 2002-07-16 University Of Washington Chemical synthesis using solvent microdroplets
JP4245084B2 (ja) 1997-03-21 2009-03-25 ストラタジーン ポリメラーゼ増強因子（ｐｅｆ）抽出物、ｐｅｆタンパク質複合体、単離されたｐｅｆタンパク質、並びに精製及び単離方法
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5922593A (en) 1997-05-23 1999-07-13 Becton, Dickinson And Company Microbiological test panel and method therefor
EP0991930B1 (en) 1997-06-26 2004-06-16 Perseptive Biosystems, Inc. High density sample holder for analysis of biological samples
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US6027898A (en) 1997-08-18 2000-02-22 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6136568A (en) 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
ES2243007T3 (es) 1997-09-16 2005-11-16 Egea Biosciences Llc Sintesis quimica completa y sintesis de genes de genomas.
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5976842A (en) 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6408308B1 (en) 1998-01-29 2002-06-18 Incyte Pharmaceuticals, Inc. System and method for generating, analyzing and storing normalized expression datasets from raw expression datasets derived from microarray includes nucleic acid probe sequences
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
US6251588B1 (en) 1998-02-10 2001-06-26 Agilent Technologies, Inc. Method for evaluating oligonucleotide probe sequences
JP4503828B2 (ja) 1998-02-11 2010-07-14 ユニバーシティー オブ ヒューストン 光生成試薬を用いる化学反応および生化学反応のための方法および装置
US20040035690A1 (en) * 1998-02-11 2004-02-26 The Regents Of The University Of Michigan Method and apparatus for chemical and biochemical reactions using photo-generated reagents
DE69942272D1 (de) 1998-02-23 2010-06-02 Wisconsin Alumni Res Found Deranordnungen
CA2325468C (en) 1998-03-25 2008-11-18 Ulf Landegren Rolling circle replication of padlock probes
US6284497B1 (en) 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
AU762397B2 (en) 1998-04-13 2003-06-26 Isis Pharmaceuticals, Inc. Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
US7321828B2 (en) 1998-04-13 2008-01-22 Isis Pharmaceuticals, Inc. System of components for preparing oligonucleotides
US6376285B1 (en) 1998-05-28 2002-04-23 Texas Instruments Incorporated Annealed porous silicon with epitaxial layer for SOI
US6274725B1 (en) 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
US6130045A (en) 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
US6251595B1 (en) 1998-06-18 2001-06-26 Agilent Technologies, Inc. Methods and devices for carrying out chemical reactions
ATE313548T1 (de) 1998-06-22 2006-01-15 Affymetrix Inc Reagenz und verfahren zu fester phase synthese
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
US7399844B2 (en) 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US6222030B1 (en) 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6107038A (en) 1998-08-14 2000-08-22 Agilent Technologies Inc. Method of binding a plurality of chemicals on a substrate by electrophoretic self-assembly
ATE481637T1 (de) 1998-08-28 2010-10-15 Febit Holding Gmbh Verfahren zur herstellung von biochemischen reaktionsträgern
US6258454B1 (en) 1998-09-01 2001-07-10 Agilent Technologies Inc. Functionalization of substrate surfaces with silane mixtures
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6461812B2 (en) 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
AU770993B2 (en) 1998-09-15 2004-03-11 Yale University Molecular cloning using rolling circle amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6399516B1 (en) 1998-10-30 2002-06-04 Massachusetts Institute Of Technology Plasma etch techniques for fabricating silicon structures from a substrate
US6309828B1 (en) 1998-11-18 2001-10-30 Agilent Technologies, Inc. Method and apparatus for fabricating replicate arrays of nucleic acid molecules
CA2356946A1 (en) 1998-12-31 2000-07-06 Glenn Hoke Assay device comprising mixed probes
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
WO2000042559A1 (en) 1999-01-18 2000-07-20 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
JP2002534965A (ja) 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド 所望の特徴を有する文字ストリング、ポリヌクレオチドおよびポリペプチドを作製するための方法
US6251685B1 (en) 1999-02-18 2001-06-26 Agilent Technologies, Inc. Readout method for molecular biological electronically addressable arrays
WO2000049142A1 (de) 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
WO2000050642A1 (en) 1999-02-23 2000-08-31 Caliper Technologies Corp. Sequencing by incorporation
JP2002538790A (ja) * 1999-03-08 2002-11-19 プロトジーン・ラボラトリーズ・インコーポレーテッド 長いｄｎａ配列を経済的に合成し、そして組み立てるための方法および組成物
US6824866B1 (en) 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US6284465B1 (en) 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US6469156B1 (en) 1999-04-20 2002-10-22 The United States Of America As Represented By The Department Of Health And Human Services Rapid and sensitive method for detecting histoplasma capsulatum
US6518056B2 (en) 1999-04-27 2003-02-11 Agilent Technologies Inc. Apparatus, systems and method for assaying biological materials using an annular format
US6773676B2 (en) 1999-04-27 2004-08-10 Agilent Technologies, Inc. Devices for performing array hybridization assays and methods of using the same
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6300137B1 (en) 1999-04-28 2001-10-09 Agilent Technologies Inc. Method for synthesizing a specific, surface-bound polymer uniformly over an element of a molecular array
US6323043B1 (en) 1999-04-30 2001-11-27 Agilent Technologies, Inc. Fabricating biopolymer arrays
US7276336B1 (en) 1999-07-22 2007-10-02 Agilent Technologies, Inc. Methods of fabricating an addressable array of biopolymer probes
US6242266B1 (en) 1999-04-30 2001-06-05 Agilent Technologies Inc. Preparation of biopolymer arrays
WO2000066190A1 (en) 1999-05-01 2000-11-09 Qinetiq Limited Derivatized porous silicon
AU4698600A (en) * 1999-05-05 2000-11-17 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
AU4703300A (en) 1999-05-06 2000-11-21 Mount Sinai School Of Medicine Of The City University Of New York, The Dna-based steganography
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1185544B1 (en) 1999-05-24 2008-11-26 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US6472147B1 (en) 1999-05-25 2002-10-29 The Scripps Research Institute Methods for display of heterodimeric proteins on filamentous phage using pVII and pIX, compositions, vectors and combinatorial libraries
US6132997A (en) 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6815218B1 (en) 1999-06-09 2004-11-09 Massachusetts Institute Of Technology Methods for manufacturing bioelectronic devices
JP2003510017A (ja) 1999-06-22 2003-03-18 インビトロジェン コーポレイション 核酸の検出および識別のために改良されたプライマーおよび方法
US6709852B1 (en) 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
DE19928410C2 (de) 1999-06-22 2002-11-28 Agilent Technologies Inc Gerätegehäuse mit einer Einrichtung zum Betrieb eines Labor-Mikrochips
US6399394B1 (en) 1999-06-30 2002-06-04 Agilent Technologies, Inc. Testing multiple fluid samples with multiple biopolymer arrays
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US7504213B2 (en) 1999-07-09 2009-03-17 Agilent Technologies, Inc. Methods and apparatus for preparing arrays comprising features having degenerate biopolymers
US6461816B1 (en) 1999-07-09 2002-10-08 Agilent Technologies, Inc. Methods for controlling cross-hybridization in analysis of nucleic acid sequences
US6346423B1 (en) 1999-07-16 2002-02-12 Agilent Technologies, Inc. Methods and compositions for producing biopolymeric arrays
US6306599B1 (en) 1999-07-16 2001-10-23 Agilent Technologies Inc. Biopolymer arrays and their fabrication
US6201112B1 (en) 1999-07-22 2001-03-13 Agilent Technologies Inc. Method for 3′ end-labeling ribonucleic acids
US6180351B1 (en) 1999-07-22 2001-01-30 Agilent Technologies Inc. Chemical array fabrication with identifier
CA2382157C (en) 1999-08-18 2012-04-03 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
US6262490B1 (en) 1999-11-05 2001-07-17 Advanced Semiconductor Engineering, Inc. Substrate strip for use in packaging semiconductor chips
US6743585B2 (en) 1999-09-16 2004-06-01 Agilent Technologies, Inc. Methods for preparing conjugates
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6319674B1 (en) 1999-09-16 2001-11-20 Agilent Technologies, Inc. Methods for attaching substances to surfaces
US7078167B2 (en) 1999-09-17 2006-07-18 Agilent Technologies, Inc. Arrays having background features and methods for using the same
US7122303B2 (en) 1999-09-17 2006-10-17 Agilent Technologies, Inc. Arrays comprising background features that provide for a measure of a non-specific binding and methods for using the same
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
DE19947495C2 (de) 1999-10-01 2003-05-28 Agilent Technologies Inc Mikrofluidischer Mikrochip
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6232072B1 (en) 1999-10-15 2001-05-15 Agilent Technologies, Inc. Biopolymer array inspection
US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
US6171797B1 (en) 1999-10-20 2001-01-09 Agilent Technologies Inc. Methods of making polymeric arrays
US7115423B1 (en) 1999-10-22 2006-10-03 Agilent Technologies, Inc. Fluidic structures within an array package
US6387636B1 (en) 1999-10-22 2002-05-14 Agilent Technologies, Inc. Method of shielding biosynthesis reactions from the ambient environment on an array
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US20010055761A1 (en) 1999-10-29 2001-12-27 Agilent Technologies Small scale dna synthesis using polymeric solid support with functionalized regions
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US6406849B1 (en) 1999-10-29 2002-06-18 Agilent Technologies, Inc. Interrogating multi-featured arrays
US6329210B1 (en) 1999-10-29 2001-12-11 Agilent Technologies, Inc. Method and apparatus for high volume polymer synthesis
US6689319B1 (en) 1999-10-29 2004-02-10 Agilent Technologies, Ind. Apparatus for deposition and inspection of chemical and biological fluids
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US6440669B1 (en) 1999-11-10 2002-08-27 Agilent Technologies, Inc. Methods for applying small volumes of reagents
US7041445B2 (en) 1999-11-15 2006-05-09 Clontech Laboratories, Inc. Long oligonucleotide arrays
US6446642B1 (en) 1999-11-22 2002-09-10 Agilent Technologies, Inc. Method and apparatus to clean an inkjet reagent deposition device
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
JP2003519495A (ja) 2000-01-11 2003-06-24 マキシジェン， インコーポレイテッド 多様性生成およびスクリーニングのための一体化されたシステムおよび方法
EP1118661A1 (en) 2000-01-13 2001-07-25 Het Nederlands Kanker Instituut T cell receptor libraries
EP1252513A4 (en) 2000-01-25 2007-07-18 Affymetrix Inc METHOD, SYSTEM AND SOFTWARE FOR OFFERING A GENOMIC WEB PORTAL
US6587579B1 (en) 2000-01-26 2003-07-01 Agilent Technologies Inc. Feature quality in array fabrication
US7198939B2 (en) 2000-01-28 2007-04-03 Agilent Technologies, Inc. Apparatus for interrogating an addressable array
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid
US6406851B1 (en) 2000-01-28 2002-06-18 Agilent Technologies, Inc. Method for coating a substrate quickly and uniformly with a small volume of fluid
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US6403314B1 (en) 2000-02-04 2002-06-11 Agilent Technologies, Inc. Computational method and system for predicting fragmented hybridization and for identifying potential cross-hybridization
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6365355B1 (en) 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
US7776021B2 (en) 2000-04-28 2010-08-17 The Charles Stark Draper Laboratory Micromachined bilayer unit for filtration of small molecules
US6716634B1 (en) 2000-05-31 2004-04-06 Agilent Technologies, Inc. Increasing ionization efficiency in mass spectrometry
US7163660B2 (en) 2000-05-31 2007-01-16 Infineon Technologies Ag Arrangement for taking up liquid analytes
WO2001094366A1 (en) 2000-06-02 2001-12-13 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US6686193B2 (en) 2000-07-10 2004-02-03 Vertex Pharmaceuticals, Inc. High throughput method and system for screening candidate compounds for activity against target ion channels
NZ523715A (en) 2000-07-27 2004-07-30 Univ Australian A set of oligonucleotide probes 'promiscuous' probes that hybridise to target sequences common to more than one of the target polynucleotides
US7135565B2 (en) 2000-07-28 2006-11-14 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
US7205400B2 (en) 2000-07-31 2007-04-17 Agilent Technologies, Inc. Array fabrication
US6599693B1 (en) 2000-07-31 2003-07-29 Agilent Technologies Inc. Array fabrication
US6890760B1 (en) 2000-07-31 2005-05-10 Agilent Technologies, Inc. Array fabrication
EP1598432A3 (en) 2000-07-31 2006-06-07 Agilent Technologies, Inc. Array based methods for sythesizing nucleic acid mixtures
US6613893B1 (en) 2000-07-31 2003-09-02 Agilent Technologies Inc. Array fabrication
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
WO2002016583A2 (en) 2000-08-24 2002-02-28 Maxygen, Inc. Constructs and their use in metabolic pathway engineering
JP2004507565A (ja) 2000-09-08 2004-03-11 ユニバーシティー・テクノロジーズ・インターナショナル・インコーポレイテッド オリゴヌクレオチド合成用のリンカーホスホルアミダイト
US6966945B1 (en) 2000-09-20 2005-11-22 Goodrich Corporation Inorganic matrix compositions, composites and process of making the same
AU2001293163A1 (en) 2000-09-27 2002-04-08 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
NO20004869D0 (no) 2000-09-28 2000-09-28 Torbjoern Rognes Metode for hurtig optimal lokal sekvensjustering ved bruk av parallell prosessering
US7097809B2 (en) 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
AU2001296809A1 (en) 2000-10-10 2002-04-22 Biotrove, Inc. Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
DE10051396A1 (de) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh Verfahren und Vorrichtung zur integrierten Synthese und Analytbestimmung an einem Träger
US6693187B1 (en) 2000-10-17 2004-02-17 Lievre Cornu Llc Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge
JP2004512607A (ja) 2000-10-18 2004-04-22 ウルトラ　プロイズヴォドニャ　エレクトロンスキ　ナプラウ　デー．オー．オー． 支払いデータ交換システム及びそのシステムにて用いられる支払い端末装置
EP1203945B1 (en) 2000-10-26 2006-12-20 Agilent Technologies, Inc. (a Delaware corporation) Microarray
US6905816B2 (en) 2000-11-27 2005-06-14 Intelligent Medical Devices, Inc. Clinically intelligent diagnostic devices and methods
US20020155439A1 (en) 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
CA2714353A1 (en) 2000-12-05 2002-06-13 Avecia Biotechnology Inc Process for the preparation of phosphorothioate oligonucleotides
US20040253242A1 (en) 2000-12-05 2004-12-16 Bowdish Katherine S. Rationally designed antibodies
DE10060433B4 (de) 2000-12-05 2006-05-11 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Verfahren zur Herstellung eines Fluidbauelements, Fluidbauelement und Analysevorrichtung
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
AUPR259301A0 (en) 2001-01-18 2001-02-15 Polymerat Pty Ltd Polymers having co-continuous architecture
DK1385950T3 (da) 2001-01-19 2008-11-03 Centocor Inc Computerstyret samling af et polynucleotid, som koder for et målpolypeptid
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US7027930B2 (en) 2001-01-31 2006-04-11 Agilent Technologies, Inc. Reading chemical arrays
US7166258B2 (en) 2001-01-31 2007-01-23 Agilent Technologies, Inc. Automation-optimized microarray package
US6879915B2 (en) 2001-01-31 2005-04-12 Agilent Technologies, Inc. Chemical array fabrication and use
US20020164824A1 (en) 2001-02-16 2002-11-07 Jianming Xiao Method and apparatus based on bundled capillaries for high throughput screening
US6660338B1 (en) 2001-03-08 2003-12-09 Agilent Technologies, Inc. Functionalization of substrate surfaces with silane mixtures
JP2004521628A (ja) 2001-03-08 2004-07-22 アプレラ コーポレイション オリゴヌクレオチドの切断および脱保護のための試薬
US7211654B2 (en) * 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
EP2801624B1 (en) 2001-03-16 2019-03-06 Singular Bio, Inc Arrays and methods of use
US6610978B2 (en) 2001-03-27 2003-08-26 Agilent Technologies, Inc. Integrated sample preparation, separation and introduction microdevice for inductively coupled plasma mass spectrometry
US7179659B2 (en) 2001-04-02 2007-02-20 Agilent Technologies, Inc. Sensor surfaces for detecting analytes and methods of use
US20030022240A1 (en) 2001-04-17 2003-01-30 Peizhi Luo Generation and affinity maturation of antibody library in silico
US6943036B2 (en) 2001-04-30 2005-09-13 Agilent Technologies, Inc. Error detection in chemical array fabrication
JP2004530879A (ja) 2001-05-03 2004-10-07 シグマ−ジェノシス リミテッド タンパク質マイクロアレイを構築する方法
ATE403013T1 (de) 2001-05-18 2008-08-15 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
US20040175710A1 (en) 2001-05-22 2004-09-09 Haushalter Robert C. Method for in situ, on-chip chemical synthesis
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US6649348B2 (en) 2001-06-29 2003-11-18 Agilent Technologies Inc. Methods for manufacturing arrays
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US6989267B2 (en) 2001-07-02 2006-01-24 Agilent Technologies, Inc. Methods of making microarrays with substrate surfaces having covalently bound polyelectrolyte films
US6753145B2 (en) 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
US6702256B2 (en) 2001-07-17 2004-03-09 Agilent Technologies, Inc. Flow-switching microdevice
US7128876B2 (en) 2001-07-17 2006-10-31 Agilent Technologies, Inc. Microdevice and method for component separation in a fluid
US7314599B2 (en) 2001-07-17 2008-01-01 Agilent Technologies, Inc. Paek embossing and adhesion for microfluidic devices
US20030108903A1 (en) 2001-07-19 2003-06-12 Liman Wang Multiple word DNA computing on surfaces
ATE432981T1 (de) 2001-07-26 2009-06-15 Stratagene California Multi-stellen-mutagenese
WO2003014325A2 (en) 2001-08-10 2003-02-20 Xencor Protein design automation for protein libraries
US7371580B2 (en) 2001-08-24 2008-05-13 Agilent Technologies, Inc. Use of unstructured nucleic acids in assaying nucleic acid molecules
US6682702B2 (en) 2001-08-24 2004-01-27 Agilent Technologies, Inc. Apparatus and method for simultaneously conducting multiple chemical reactions
JP2003101204A (ja) 2001-09-25 2003-04-04 Nec Kansai Ltd 配線基板及び配線基板の製造方法並びに電子部品
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050124022A1 (en) 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6858720B2 (en) 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US6852850B2 (en) 2001-10-31 2005-02-08 Agilent Technologies, Inc. Use of ionic liquids for fabrication of polynucleotide arrays
US7524950B2 (en) 2001-10-31 2009-04-28 Agilent Technologies, Inc. Uses of cationic salts for polynucleotide synthesis
WO2003040410A1 (en) 2001-11-02 2003-05-15 Nimblegen Systems, Inc. Detection of hybridization oligonucleotide microarray through covalently labeling microarray probe
WO2003046508A2 (en) 2001-11-09 2003-06-05 Biomicroarrays, Inc. High surface area substrates for microarrays and methods to make same
US7482118B2 (en) 2001-11-15 2009-01-27 Third Wave Technologies, Inc. Endonuclease-substrate complexes
ATE414767T1 (de) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nukleinsäure-linker und deren verwendung in der gensynthese
US20030099952A1 (en) 2001-11-26 2003-05-29 Roland Green Microarrays with visible pattern detection
US20030143605A1 (en) 2001-12-03 2003-07-31 Si Lok Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
US6838888B2 (en) 2001-12-13 2005-01-04 Agilent Technologies, Inc. Flow cell humidity sensor system
WO2003054232A2 (en) 2001-12-13 2003-07-03 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US7282183B2 (en) 2001-12-24 2007-10-16 Agilent Technologies, Inc. Atmospheric control in reaction chambers
US6790620B2 (en) 2001-12-24 2004-09-14 Agilent Technologies, Inc. Small volume chambers
US6846454B2 (en) 2001-12-24 2005-01-25 Agilent Technologies, Inc. Fluid exit in reaction chambers
US7025324B1 (en) 2002-01-04 2006-04-11 Massachusetts Institute Of Technology Gating apparatus and method of manufacture
US20030171325A1 (en) * 2002-01-04 2003-09-11 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
US6673552B2 (en) 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
AU2003217207A1 (en) 2002-01-14 2003-07-30 Verenium Corporation Methods for making polynucleotides and purifying double-stranded polynucleotides
US20040009498A1 (en) 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
US7141368B2 (en) 2002-01-30 2006-11-28 Agilent Technologies, Inc. Multi-directional deposition in array fabrication
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
US7037659B2 (en) 2002-01-31 2006-05-02 Nimblegen Systems Inc. Apparatus for constructing DNA probes having a prismatic and kaleidoscopic light homogenizer
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
US20040126757A1 (en) 2002-01-31 2004-07-01 Francesco Cerrina Method and apparatus for synthesis of arrays of DNA probes
US7083975B2 (en) 2002-02-01 2006-08-01 Roland Green Microarray synthesis instrument and method
US20030148291A1 (en) 2002-02-05 2003-08-07 Karla Robotti Method of immobilizing biologically active molecules for assay purposes in a microfluidic format
US6728129B2 (en) 2002-02-19 2004-04-27 The Regents Of The University Of California Multistate triple-decker dyads in three distinct architectures for information storage applications
US6958119B2 (en) 2002-02-26 2005-10-25 Agilent Technologies, Inc. Mobile phase gradient generation microfluidic device
US6929951B2 (en) 2002-02-28 2005-08-16 Agilent Technologies, Inc. Method and system for molecular array scanner calibration
US6914229B2 (en) 2002-02-28 2005-07-05 Agilent Technologies, Inc. Signal offset for prevention of data clipping in a molecular array scanner
US6770892B2 (en) 2002-02-28 2004-08-03 Agilent Technologies, Inc. Method and system for automated focus-distance determination for molecular array scanners
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US6919181B2 (en) 2002-03-25 2005-07-19 Agilent Technologies, Inc. Methods for generating ligand arrays
EP1499626A2 (en) 2002-04-01 2005-01-26 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
EP1350853A1 (en) 2002-04-05 2003-10-08 ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V. Detection of polymorphisms
US6773888B2 (en) 2002-04-08 2004-08-10 Affymetrix, Inc. Photoactivatable silane compounds and methods for their synthesis and use
EP1501947B1 (en) 2002-04-22 2008-07-16 Genencor International, Inc. Method of creating a library of bacterial clones with varying levels of gene expression
GB0209539D0 (en) 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
US6946285B2 (en) 2002-04-29 2005-09-20 Agilent Technologies, Inc. Arrays with elongated features
US7125523B2 (en) 2002-04-29 2006-10-24 Agilent Technologies, Inc. Holders for arrays
US6621076B1 (en) 2002-04-30 2003-09-16 Agilent Technologies, Inc. Flexible assembly for transporting sample fluids into a mass spectrometer
US7094537B2 (en) 2002-04-30 2006-08-22 Agilent Technologies, Inc. Micro arrays with structured and unstructured probes
WO2003093504A1 (de) 2002-05-06 2003-11-13 Noxxon Pharma Ag Verfahren zur amplifikation von nukleinsäuren
US20030211478A1 (en) 2002-05-08 2003-11-13 Gentel Corporation Transcription factor profiling on a solid surface
US7221785B2 (en) 2002-05-21 2007-05-22 Agilent Technologies, Inc. Method and system for measuring a molecular array background signal from a continuous background region of specified size
CA2487093A1 (en) 2002-05-24 2003-12-04 Nimblegen Systems, Inc. Microarrays and method for running hybridization reaction for multiple samples on a single microarray
AU2003240795A1 (en) 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
US6789965B2 (en) 2002-05-31 2004-09-14 Agilent Technologies, Inc. Dot printer with off-axis loading
US7537936B2 (en) 2002-05-31 2009-05-26 Agilent Technologies, Inc. Method of testing multiple fluid samples with multiple biopolymer arrays
US7078505B2 (en) 2002-06-06 2006-07-18 Agilent Technologies, Inc. Manufacture of arrays with varying deposition parameters
US7371348B2 (en) 2002-06-14 2008-05-13 Agilent Technologies Multiple array format
US7351379B2 (en) 2002-06-14 2008-04-01 Agilent Technologies, Inc. Fluid containment structure
US7919308B2 (en) 2002-06-14 2011-04-05 Agilent Technologies, Inc. Form in place gaskets for assays
US6939673B2 (en) 2002-06-14 2005-09-06 Agilent Technologies, Inc. Manufacture of arrays with reduced error impact
US20070275411A1 (en) * 2006-05-25 2007-11-29 Mcgall Glenn H Silane mixtures
US7220573B2 (en) 2002-06-21 2007-05-22 Agilent Technologies, Inc. Array assay devices and methods of using the same
US6713262B2 (en) 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
US7894998B2 (en) 2002-06-26 2011-02-22 Agilent Technologies, Inc. Method for identifying suitable nucleic acid probe sequences for use in nucleic acid arrays
US7202358B2 (en) 2002-07-25 2007-04-10 Agilent Technologies, Inc. Methods for producing ligand arrays
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7101508B2 (en) 2002-07-31 2006-09-05 Agilent Technologies, Inc. Chemical array fabrication errors
US6835938B2 (en) 2002-07-31 2004-12-28 Agilent Technologies, Inc. Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners
US7153689B2 (en) 2002-08-01 2006-12-26 Agilent Technologies, Inc. Apparatus and methods for cleaning and priming droplet dispensing devices
US8946387B2 (en) 2002-08-14 2015-02-03 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US7205128B2 (en) 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20040166567A1 (en) 2002-09-26 2004-08-26 Santi Daniel V Synthetic genes
WO2004029586A1 (en) 2002-09-27 2004-04-08 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
WO2004031399A2 (en) 2002-09-30 2004-04-15 Nimblegen Systems, Inc. Parallel loading of arrays
CA2500783C (en) 2002-10-01 2012-07-17 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
US7129075B2 (en) 2002-10-18 2006-10-31 Transgenomic, Inc. Isolated CEL II endonuclease
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
WO2004039953A2 (en) 2002-10-28 2004-05-13 Xeotron Corporation Array oligomer synthesis and use.
US7390457B2 (en) 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
US6976384B2 (en) 2002-10-31 2005-12-20 Nanostream, Inc. Parallel detection chromatography systems
US7364896B2 (en) 2002-10-31 2008-04-29 Agilent Technologies, Inc. Test strips including flexible array substrates and method of hybridization
US7422911B2 (en) 2002-10-31 2008-09-09 Agilent Technologies, Inc. Composite flexible array substrate having flexible support
US7629120B2 (en) 2002-10-31 2009-12-08 Rice University Method for assembling PCR fragments of DNA
US7402279B2 (en) 2002-10-31 2008-07-22 Agilent Technologies, Inc. Device with integrated microfluidic and electronic components
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
US7029854B2 (en) 2002-11-22 2006-04-18 Agilent Technologies, Inc. Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays
US7062385B2 (en) 2002-11-25 2006-06-13 Tufts University Intelligent electro-optical nucleic acid-based sensor array and method for detecting volatile compounds in ambient air
RU2377253C2 (ru) 2002-12-02 2009-12-27 Амген Фремонт,Инк. Антитела, специфичные к фактору некроза опухолей, и их применение
US20040110133A1 (en) 2002-12-06 2004-06-10 Affymetrix, Inc. Functionated photoacid generator for biological microarray synthesis
US7879580B2 (en) 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US20060076482A1 (en) 2002-12-13 2006-04-13 Hobbs Steven E High throughput systems and methods for parallel sample analysis
US6987263B2 (en) 2002-12-13 2006-01-17 Nanostream, Inc. High throughput systems and methods for parallel sample analysis
US7247337B1 (en) 2002-12-16 2007-07-24 Agilent Technologies, Inc. Method and apparatus for microarray fabrication
US20040191810A1 (en) 2002-12-17 2004-09-30 Affymetrix, Inc. Immersed microarrays in conical wells
GB0229443D0 (en) 2002-12-18 2003-01-22 Avecia Ltd Process
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
ES2246748T1 (es) * 2002-12-23 2006-03-01 Agilent Technologies, Inc. Ensayos de hibridacion genomica comparativos que utilizan caracteristicas oligonucleotidicas inmovilizadas y composiciones para ponerlos en practica.
MXPA05006828A (es) 2002-12-23 2005-09-08 Wyeth Corp Anticuerpos contra pd-1, y sus usos.
DE10260805A1 (de) 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
US7737089B2 (en) 2002-12-23 2010-06-15 Febit Holding Gmbh Photoactivatable two-stage protective groups for the synthesis of biopolymers
US7372982B2 (en) 2003-01-14 2008-05-13 Agilent Technologies, Inc. User interface for molecular array feature analysis
US6809277B2 (en) 2003-01-22 2004-10-26 Agilent Technologies, Inc. Method for registering a deposited material with channel plate channels, and switch produced using same
CA2513541A1 (en) 2003-01-29 2004-08-19 454 Corporation Method for preparing single-stranded dna libraries
US7202264B2 (en) 2003-01-31 2007-04-10 Isis Pharmaceuticals, Inc. Supports for oligomer synthesis
US8073626B2 (en) 2003-01-31 2011-12-06 Agilent Technologies, Inc. Biopolymer array reading
US6950756B2 (en) 2003-02-05 2005-09-27 Agilent Technologies, Inc. Rearrangement of microarray scan images to form virtual arrays
US7413709B2 (en) 2003-02-12 2008-08-19 Agilent Technologies, Inc. PAEK-based microfluidic device with integrated electrospray emitter
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7244513B2 (en) 2003-02-21 2007-07-17 Nano-Proprietary, Inc. Stain-etched silicon powder
US7252938B2 (en) 2003-02-25 2007-08-07 Agilent Technologies, Inc. Methods and devices for producing a polymer at a location of a substrate
US7070932B2 (en) 2003-02-25 2006-07-04 Agilent Technologies, Inc. Methods and devices for detecting printhead misalignment of an in situ polymeric array synthesis device
WO2004080887A1 (en) 2003-03-07 2004-09-23 Massachusetts Institute Of Technology Three dimensional mecrofabrication
US20050053968A1 (en) 2003-03-31 2005-03-10 Council Of Scientific And Industrial Research Method for storing information in DNA
US7534561B2 (en) 2003-04-02 2009-05-19 Agilent Technologies, Inc. Nucleic acid array in situ fabrication methods and arrays produced using the same
EP1613776A1 (en) 2003-04-02 2006-01-11 Blue Heron Biotechnology, Inc. Error reduction in automated gene synthesis
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US20040219663A1 (en) 2003-04-30 2004-11-04 Page Robert D. Biopolymer array fabrication using different drop deposition heads
US7206439B2 (en) 2003-04-30 2007-04-17 Agilent Technologies, Inc. Feature locations in array reading
US6916113B2 (en) 2003-05-16 2005-07-12 Agilent Technologies, Inc. Devices and methods for fluid mixing
EP1636017A2 (en) 2003-05-20 2006-03-22 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
NZ544952A (en) 2003-05-30 2009-12-24 Univ Illinois Gene expression profiles that identify genetically elite ungulate mammals
WO2004108081A2 (en) 2003-06-02 2004-12-16 Isis Pharmaceuticals, Inc. Oligonucleotide synthesis with alternative solvents
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
US6938476B2 (en) 2003-06-25 2005-09-06 Agilent Technologies, Inc. Apparatus and methods for sensing fluid levels
US7534563B2 (en) 2003-06-30 2009-05-19 Agilent Technologies, Inc. Methods for producing ligand arrays
US20050016851A1 (en) 2003-07-24 2005-01-27 Jensen Klavs F. Microchemical method and apparatus for synthesis and coating of colloidal nanoparticles
US6843281B1 (en) 2003-07-30 2005-01-18 Agilent Techinologies, Inc. Methods and apparatus for introducing liquids into microfluidic chambers
US7353116B2 (en) 2003-07-31 2008-04-01 Agilent Technologies, Inc. Chemical array with test dependent signal reading or processing
US7939310B2 (en) 2003-08-06 2011-05-10 University Of Massachusetts Systems and methods for analyzing nucleic acid sequences
US7028536B2 (en) 2004-06-29 2006-04-18 Nanostream, Inc. Sealing interface for microfluidic device
US7348144B2 (en) 2003-08-13 2008-03-25 Agilent Technologies, Inc. Methods and system for multi-drug treatment discovery
US7229497B2 (en) 2003-08-26 2007-06-12 Massachusetts Institute Of Technology Method of preparing nanocrystals
US7417139B2 (en) 2003-08-30 2008-08-26 Agilent Technologies, Inc. Method for polynucleotide synthesis
US7427679B2 (en) 2003-08-30 2008-09-23 Agilent Technologies, Inc. Precursors for two-step polynucleotide synthesis
US7585970B2 (en) 2003-08-30 2009-09-08 Agilent Technologies, Inc. Method of polynucleotide synthesis using modified support
US7193077B2 (en) 2003-08-30 2007-03-20 Agilent Technologies, Inc. Exocyclic amine triaryl methyl protecting groups in two-step polynucleotide synthesis
US7385050B2 (en) 2003-08-30 2008-06-10 Agilent Technologies, Inc. Cleavable linker for polynucleotide synthesis
US20050049796A1 (en) 2003-09-03 2005-03-03 Webb Peter G. Methods for encoding non-biological information on microarrays
US7362901B2 (en) 2003-09-05 2008-04-22 Gannon Technology Holdings Llc Systems and methods for biometric identification using handwriting recognition
US20120202710A1 (en) 2003-09-09 2012-08-09 Integrigen, Inc. Methods and compositions for generation of germline human antibody genes
US20050112636A1 (en) 2003-09-23 2005-05-26 Atom Sciences Polymeric nucleic acid hybridization probes
US7488607B2 (en) 2003-09-30 2009-02-10 Agilent Technologies, Inc. Electronically readable microarray with electronic addressing function
US7147362B2 (en) 2003-10-15 2006-12-12 Agilent Technologies, Inc. Method of mixing by intermittent centrifugal force
US7075161B2 (en) 2003-10-23 2006-07-11 Agilent Technologies, Inc. Apparatus and method for making a low capacitance artificial nanopore
US20050277125A1 (en) 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7276338B2 (en) 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
DE10353887A1 (de) 2003-11-18 2005-06-16 Febit Ag Hochparalleler DNA-Synthesizer auf Matrizenbasis
US7851192B2 (en) 2004-11-22 2010-12-14 New England Biolabs, Inc. Modified DNA cleavage enzymes and methods for use
US7282705B2 (en) 2003-12-19 2007-10-16 Agilent Technologies, Inc. Microdevice having an annular lining for producing an electrospray emitter
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
US7084180B2 (en) 2004-01-28 2006-08-01 Velocys, Inc. Fischer-tropsch synthesis using microchannel technology and novel catalyst and microchannel reactor
CA2553833C (en) 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
AU2005214329A1 (en) 2004-02-12 2005-09-01 Population Genetics Technologies Ltd Genetic analysis by sequence-specific sorting
US7125488B2 (en) 2004-02-12 2006-10-24 Varian, Inc. Polar-modified bonded phase materials for chromatographic separations
AU2005222788A1 (en) 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7875463B2 (en) 2004-03-26 2011-01-25 Agilent Technologies, Inc. Generalized pulse jet ejection head control model
WO2005093092A2 (en) 2004-03-26 2005-10-06 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with g-protein coupled receptor 44 (gpr44)
US20050214778A1 (en) 2004-03-29 2005-09-29 Peck Bill J Methods for in situ generation of nucleic acid arrays
US20050214779A1 (en) 2004-03-29 2005-09-29 Peck Bill J Methods for in situ generation of nucleic acid arrays
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
US20060010513A1 (en) 2004-05-11 2006-01-12 Melville Mark W Oligonucleotide arrays to monitor gene expression and methods for making and using same
ES2459367T3 (es) 2004-05-19 2014-05-09 Massachusetts Institute Of Technology Modelos tridimensionales de enfermedades de células/tejidos perfundidos
US7302348B2 (en) 2004-06-02 2007-11-27 Agilent Technologies, Inc. Method and system for quantifying and removing spatial-intensity trends in microarray data
US20060002471A1 (en) 2004-06-30 2006-01-05 Lippincott Louis A Motion estimation unit
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
EP1774019B1 (en) 2004-07-06 2011-10-12 Bioren, Inc. Look-through mutagenesis for developing altered polypeptides with enhanced properties
WO2006014533A2 (en) 2004-07-07 2006-02-09 Home Guardian Llc Instrumented mobility assistance device
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060019084A1 (en) 2004-07-23 2006-01-26 Pearson Laurence T Monolithic composition and method
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
JP4550115B2 (ja) 2004-08-03 2010-09-22 ジーンアート・アクチエンゲゼルシャフト ＣｐＧ含量を変化させることにより遺伝子発現を調整する方法
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US7034290B2 (en) 2004-09-24 2006-04-25 Agilent Technologies, Inc. Target support with pattern recognition sites
US7943046B2 (en) 2004-10-01 2011-05-17 Agilent Technologies, Inc Methods and systems for on-column protein delipidation
US20070122817A1 (en) 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
WO2006044956A1 (en) 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US7141807B2 (en) 2004-10-22 2006-11-28 Agilent Technologies, Inc. Nanowire capillaries for mass spectrometry
US20060110744A1 (en) 2004-11-23 2006-05-25 Sampas Nicolas M Probe design methods and microarrays for comparative genomic hybridization and location analysis
US8380441B2 (en) 2004-11-30 2013-02-19 Agilent Technologies, Inc. Systems for producing chemical array layouts
US11268149B2 (en) 2004-12-08 2022-03-08 Cedars-Sinai Medical Center Diagnosis and treatment of inflammatory bowel disease
US7977119B2 (en) 2004-12-08 2011-07-12 Agilent Technologies, Inc. Chemical arrays and methods of using the same
US7439272B2 (en) 2004-12-20 2008-10-21 Varian, Inc. Ultraporous sol gel monoliths
EP1841788A4 (en) 2004-12-22 2009-01-21 Univ Singapore NEW TOXIN FROM A SERPENT
EP1838870A2 (en) 2004-12-29 2007-10-03 Exiqon A/S NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF MICRORNAS AND THEIR TARGET MRNAs
US20060160138A1 (en) 2005-01-13 2006-07-20 George Church Compositions and methods for protein design
US20060171855A1 (en) 2005-02-03 2006-08-03 Hongfeng Yin Devices,systems and methods for multi-dimensional separation
WO2006086391A2 (en) 2005-02-07 2006-08-17 Massachusetts Institute Of Technology Electrochemically-degradable layer-by-layer thin films
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP4641199B2 (ja) 2005-02-28 2011-03-02 国立感染症研究所長 Ｒｎａ干渉ポリヌクレオチド混合物の設計装置、ｒｎａ干渉ポリヌクレオチド混合物の作製方法、及びｒｎａ干渉ポリヌクレオチド混合物の設計プログラム
US20060203236A1 (en) 2005-03-08 2006-09-14 Zhenghua Ji Sample cell
EP1623763A1 (en) 2005-03-11 2006-02-08 Agilent Technologies, Inc. Chip with cleaning cavity
US7618777B2 (en) 2005-03-16 2009-11-17 Agilent Technologies, Inc. Composition and method for array hybridization
US20060219637A1 (en) 2005-03-29 2006-10-05 Killeen Kevin P Devices, systems and methods for liquid chromatography
WO2006116476A1 (en) 2005-04-27 2006-11-02 Sigma-Aldrich Co. Activators for oligonucleotide and phosphoramidite synthesis
AU2006242387B2 (en) 2005-04-29 2011-01-06 Synthetic Genomics, Inc. Amplification and cloning of single DNA molecules using rolling circle amplification
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
RU2435809C2 (ru) 2005-05-12 2011-12-10 Хемпель А/С Способ образования устойчивого к трещинообразованию лакокрасочного эпоксидного покрытия и лакокрасочная композиция, подходящая для такого способа
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US7919239B2 (en) 2005-07-01 2011-04-05 Agilent Technologies, Inc. Increasing hybridization efficiencies
US8076064B2 (en) 2005-07-09 2011-12-13 Agilent Technologies, Inc. Method of treatment of RNA sample
US7718365B2 (en) 2005-07-09 2010-05-18 Agilent Technologies, Inc. Microarray analysis of RNA
ATE510930T1 (de) 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
DE102005037351B3 (de) 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
US9404882B2 (en) 2005-08-11 2016-08-02 New Mexico Tech Research Foundation Method of producing a multi-microchannel, flow-through element and device using same
US7749701B2 (en) 2005-08-11 2010-07-06 Agilent Technologies, Inc. Controlling use of oligonucleotide sequences released from arrays
WO2007032837A2 (en) 2005-08-11 2007-03-22 The J. Craig Venter Institute Method for in vitro recombination
US7723077B2 (en) 2005-08-11 2010-05-25 Synthetic Genomics, Inc. In vitro recombination method
US7805252B2 (en) 2005-08-16 2010-09-28 Dna Twopointo, Inc. Systems and methods for designing and ordering polynucleotides
WO2007025059A1 (en) 2005-08-26 2007-03-01 Surmodics, Inc. Silane coating compositions, coating systems, and methods
US20070196834A1 (en) 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
CN101277758A (zh) * 2005-09-14 2008-10-01 Illumina公司 连续聚合物合成器
US20100233429A1 (en) 2005-09-16 2010-09-16 Yamatake Corporation Substrate for Biochip, Biochip, Method for Manufacturing Substrate for Biochip and Method for Manufacturing Biochip
US20080308884A1 (en) 2005-10-13 2008-12-18 Silex Microsystems Ab Fabrication of Inlet and Outlet Connections for Microfluidic Chips
US7759471B2 (en) 2005-10-31 2010-07-20 Agilent Technologies, Inc. Monomer compositions for the synthesis of RNA, methods of synthesis, and methods of deprotection
US8552174B2 (en) 2005-10-31 2013-10-08 Agilent Technologies, Inc. Solutions, methods, and processes for deprotection of polynucleotides
US8202985B2 (en) 2005-10-31 2012-06-19 Agilent Technologies, Inc. Monomer compositions for the synthesis of polynucleotides, methods of synthesis, and methods of deprotection
US7368550B2 (en) 2005-10-31 2008-05-06 Agilent Technologies, Inc. Phosphorus protecting groups
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7291471B2 (en) 2005-11-21 2007-11-06 Agilent Technologies, Inc. Cleavable oligonucleotide arrays
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US8137936B2 (en) 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
DK1966394T3 (da) 2005-12-22 2012-10-29 Keygene Nv Forbedrede strategier til transkriptprofilering under anvendelse af high throughput-sekventeringsteknologier
US7939645B2 (en) 2006-01-06 2011-05-10 Agilent Technologies, Inc Reaction buffer composition for nucleic acid replication with packed DNA polymerases
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
US7544473B2 (en) 2006-01-23 2009-06-09 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
US20070207487A1 (en) 2006-01-25 2007-09-06 Emig Christopher J Photoelectrochemical synthesis of high density combinatorial polymer arrays
WO2008057127A2 (en) 2006-02-06 2008-05-15 Massachusetts Institute Of Technology Self-assembly of macromolecules on multilayered polymer surfaces
CN101384846A (zh) 2006-02-13 2009-03-11 皇家飞利浦电子股份有限公司 用于分子诊断用途的微流体装置
WO2007095171A2 (en) 2006-02-14 2007-08-23 Massachusetts Institute Of Technology Absorbing film
US7807356B2 (en) 2006-03-09 2010-10-05 Agilent Technologies, Inc. Labeled nucleotide composition
TW200806317A (en) 2006-03-20 2008-02-01 Wyeth Corp Methods for reducing protein aggregation
US7855281B2 (en) 2006-03-23 2010-12-21 Agilent Technologies, Inc. Cleavable thiocarbonate linkers for polynucleotide synthesis
US7572908B2 (en) 2006-03-23 2009-08-11 Agilent Technologies, Inc. Cleavable linkers for polynucleotides
US20070231800A1 (en) 2006-03-28 2007-10-04 Agilent Technologies, Inc. Determination of methylated DNA
US8241573B2 (en) 2006-03-31 2012-08-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US20070238104A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Competitive oligonucleotides
US20070238108A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
EP2010567A2 (en) 2006-04-07 2009-01-07 The Government of the United States of America as Represented by The Department of Health and Human Services Antibody compositions and methods for treatment of neoplastic disease
US8058055B2 (en) 2006-04-07 2011-11-15 Agilent Technologies, Inc. High resolution chromosomal mapping
US20070238106A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Systems and methods of determining alleles and/or copy numbers
EP2010678A2 (en) 2006-04-11 2009-01-07 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
EP2007907A2 (en) * 2006-04-19 2008-12-31 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US20070259347A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Methods of increasing the effective probe densities of arrays
US20070259345A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Target determination using compound probes
US20070259344A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Compound probes and methods of increasing the effective probe densities of arrays
US20070259346A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Analysis of arrays
WO2007136834A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2007137242A2 (en) 2006-05-19 2007-11-29 Massachusetts Institute Of Technology Microfluidic-based gene synthesis
WO2008054543A2 (en) 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly
US7624114B2 (en) 2006-06-05 2009-11-24 Microsoft Corporation Automatically generating web forms from database schema
EP2035984A4 (en) 2006-06-19 2010-03-31 Yeda Res & Dev PROGRAMMABLE ITERATED ELONGATION: PROCESS FOR THE MANUFACTURE OF SYNTHETIC GENES AND COMBINATORY DNA AND PROTEIN LIBRARIES
AT503861B1 (de) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw Verfahren zur manipulation von t-zell-rezeptoren
AT503902B1 (de) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw Verfahren zur manipulation von immunglobulinen
US20080193772A1 (en) 2006-07-07 2008-08-14 Bio-Rad Laboratories, Inc Mass spectrometry probes having hydrophobic coatiings
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US7572585B2 (en) 2006-07-31 2009-08-11 Agilent Technologies, Inc. Enzymatic labeling of RNA
US7524942B2 (en) 2006-07-31 2009-04-28 Agilent Technologies, Inc. Labeled nucleotide composition
LT2056845T (lt) 2006-08-08 2017-11-27 Rheinische Friedrich-Wilhelms-Universität Bonn 5` fosfato oligonukleotidų struktūra ir panaudojimas
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
US8932994B2 (en) 2006-08-24 2015-01-13 Illumina, Inc. Method for retaining even coverage of short insert libraries
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US8415138B2 (en) 2006-08-31 2013-04-09 Agilent Technologies, Inc. Apparatuses and methods for oligonucleotide preparation
US8097711B2 (en) 2006-09-02 2012-01-17 Agilent Technologies, Inc. Thioether substituted aryl carbonate protecting groups
US20080311628A1 (en) 2006-10-03 2008-12-18 Ghc Technologies, Inc. Methods and compositions for rapid amplification and capture of nucleic acid sequences
US20080287320A1 (en) 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
US20080085511A1 (en) * 2006-10-05 2008-04-10 Peck Bill J Preparation of biopolymer arrays
US20080085514A1 (en) 2006-10-10 2008-04-10 Peck Bill J Methods and devices for array synthesis
JP2008097189A (ja) 2006-10-10 2008-04-24 National Institute Of Advanced Industrial & Technology 塩基配列断片の転写物特異性又は遺伝子特異性を判定する方法
US7867782B2 (en) 2006-10-19 2011-01-11 Agilent Technologies, Inc. Nanoscale moiety placement methods
US7999087B2 (en) 2006-11-15 2011-08-16 Agilent Technologies, Inc. 2′-silyl containing thiocarbonate protecting groups for RNA synthesis
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
WO2008063134A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Method of producing a pattern of discriminative wettability
US8242258B2 (en) 2006-12-03 2012-08-14 Agilent Technologies, Inc. Protecting groups for RNA synthesis
CN101611056A (zh) 2006-12-05 2009-12-23 埃博灵克斯股份有限公司 能够结合于血清蛋白的肽
US7989396B2 (en) 2006-12-05 2011-08-02 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule immobilization on biosensors
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US8314220B2 (en) 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA
US20080182296A1 (en) 2007-01-31 2008-07-31 Chanda Pranab K Pcr-directed gene synthesis from large number of overlapping oligodeoxyribonucleotides
KR100827449B1 (ko) 2007-02-07 2008-05-07 삼성전자주식회사 광분해성 화합물과 상기 화합물이 커플링된 올리고머프로브 어레이용 기판, 올리고머 프로브 어레이 및 이의제조 방법
US20100323404A1 (en) 2007-02-09 2010-12-23 Richard Lathrop Method for recombining dna sequences and compositions related thereto
US20090075378A1 (en) 2007-02-20 2009-03-19 Anaptysbio, Inc. Somatic hypermutation systems
JP2008218579A (ja) 2007-03-01 2008-09-18 Denki Kagaku Kogyo Kk 金属ベース回路基板
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US7651762B2 (en) 2007-03-13 2010-01-26 Varian, Inc. Methods and devices using a shrinkable support for porous monolithic materials
CN101680873B (zh) 2007-04-04 2015-11-25 加利福尼亚大学董事会 使用纳米孔的组合物、设备、系统和方法
US8202983B2 (en) 2007-05-10 2012-06-19 Agilent Technologies, Inc. Thiocarbon-protecting groups for RNA synthesis
WO2008148392A1 (en) 2007-06-04 2008-12-11 In Situ Rcp A/S Enzyme activity assay using rolling circle amplification
US20080318334A1 (en) 2007-06-20 2008-12-25 Robotti Karla M Microfluidic devices comprising fluid flow paths having a monolithic chromatographic material
US20090023190A1 (en) 2007-06-20 2009-01-22 Kai Qin Lao Sequence amplification with loopable primers
US8194244B2 (en) 2007-06-29 2012-06-05 Intel Corporation Solution sample plate with wells designed for improved Raman scattering signal detection efficiency
US7659069B2 (en) 2007-08-31 2010-02-09 Agilent Technologies, Inc. Binary signaling assay using a split-polymerase
US7979215B2 (en) 2007-07-30 2011-07-12 Agilent Technologies, Inc. Methods and systems for evaluating CGH candidate probe nucleic acid sequences
US8685642B2 (en) 2007-07-30 2014-04-01 Agilent Technologies, Inc. Allele-specific copy number measurement using single nucleotide polymorphism and DNA arrays
US20090036664A1 (en) 2007-07-31 2009-02-05 Brian Jon Peter Complex oligonucleotide primer mix
WO2009020435A1 (en) 2007-08-07 2009-02-12 Agency For Science, Technology And Research Integrated microfluidic device for gene synthesis
WO2009023547A2 (en) 2007-08-14 2009-02-19 Arcxis Biotechnologies Polymer microfluidic biochip fabrication
US20110126929A1 (en) 2007-08-15 2011-06-02 Massachusetts Institute Of Technology Microstructures For Fluidic Ballasting and Flow Control
US9598737B2 (en) 2012-05-09 2017-03-21 Longhorn Vaccines And Diagnostics, Llc Next generation genomic sequencing methods
US20090053704A1 (en) 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20100256017A1 (en) 2007-09-17 2010-10-07 Harry Benjamin Larman Supramolecular nanostamping printing device
US7790387B2 (en) 2007-09-24 2010-09-07 Agilent Technologies, Inc. Thiocarbonate linkers for polynucleotides
EP2203566A4 (en) 2007-09-28 2011-02-09 Pacific Biosciences California ERROR-FREE DNA AMPLIFICATION FOR CLONAL SEQUENCING
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009070665A1 (en) 2007-11-27 2009-06-04 Massachusetts Institute Of Technology Near field detector for integrated surface plasmon resonance biosensor applications
WO2009076580A2 (en) 2007-12-12 2009-06-18 Thomas Jefferson University Compositions and methods for the treatment and prevention of cardiovascular diseases
EP2247708A4 (en) 2007-12-17 2013-02-27 Yeda Res & Dev SYSTEM AND METHOD FOR PROCESSING AND MANIPULATING DNA
WO2012044847A1 (en) 2010-10-01 2012-04-05 Life Technologies Corporation Nucleic acid adaptors and uses thereof
CN102016068A (zh) 2008-01-09 2011-04-13 生命科技公司 制备用于核酸测序的配对标签文库的方法
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
EP2238459B1 (en) 2008-01-23 2019-05-08 Roche Diagnostics GmbH Integrated instrument performing synthesis and amplification
US8304273B2 (en) 2008-01-24 2012-11-06 Massachusetts Institute Of Technology Insulated nanogap devices and methods of use thereof
US20090194483A1 (en) 2008-01-31 2009-08-06 Robotti Karla M Microfluidic device having monolithic separation medium and method of use
CN102016070A (zh) 2008-02-15 2011-04-13 合成基因组公司 体外连接和组合装配核酸分子的方法
WO2009113709A1 (ja) 2008-03-11 2009-09-17 国立大学法人東京大学 粘着末端を有するｄｎａ断片の調製方法
US20090230044A1 (en) 2008-03-13 2009-09-17 Agilent Technologies, Inc. Microfluid Chip Cleaning
US20090238722A1 (en) 2008-03-18 2009-09-24 Agilent Technologies, Inc. Pressure-Reinforced Fluidic Chip
WO2009145818A1 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
JP4582224B2 (ja) * 2008-05-02 2010-11-17 ソニー株式会社 マイクロビーズ作製方法及びマイクロビーズ
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
JP5592357B2 (ja) 2008-05-27 2014-09-17 ダコ・デンマーク・エー／エス 新規なハイブリダイゼーションバッファーを用いた染色体異常の検出のための組成物及び方法
TWI466684B (zh) 2008-06-30 2015-01-01 Morphotek Inc 抗ｇｄ２抗體及其相關方法及用途
GB2461546B (en) 2008-07-02 2010-07-07 Argen X Bv Antigen binding polypeptides
JP4667490B2 (ja) 2008-07-09 2011-04-13 三菱電機株式会社 加熱調理器
WO2010014903A1 (en) 2008-07-31 2010-02-04 Massachusetts Institute Of Technology Multiplexed olfactory receptor-based microsurface plasmon polariton detector
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
WO2010021692A1 (en) 2008-08-22 2010-02-25 Sangamo Biosciences, Inc. Methods and compositions for targeted single-stranded cleavage and targeted integration
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8034917B2 (en) 2008-08-28 2011-10-11 Agilent Technologies, Inc. Primer-directed chromosome painting
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
CA2772434C (en) 2008-09-05 2017-11-21 Masad Damha Rna monomers containing o-acetal levulinyl ester groups and their use in rna microarrays
EP2344678A4 (en) 2008-09-05 2012-06-06 Life Technologies Corp METHODS AND SYSTEMS FOR VALIDATION, CALIBRATION AND NORMALIZATION OF NUCLEIC ACID SEQUENCING
US8541569B2 (en) 2008-09-06 2013-09-24 Chemgenes Corporation Phosphoramidites for synthetic RNA in the reverse direction, efficient RNA synthesis and convenient introduction of 3'-end ligands, chromophores and modifications of synthetic RNA
WO2010027512A2 (en) 2008-09-06 2010-03-11 Chemgenes Corporation Rna synthesis - phosphoramidites for synthetic rna in the reverse direction, and application in convenient introduction of ligands, chromophores and modifications of synthetic rna at the 3' - end
WO2010030776A1 (en) 2008-09-10 2010-03-18 Genscript Corporation Homologous recombination-based dna cloning methods and compositions
US20100076183A1 (en) 2008-09-22 2010-03-25 Dellinger Douglas J Protected monomer and method of final deprotection for rna synthesis
US8213015B2 (en) 2008-09-25 2012-07-03 Agilent Technologies, Inc. Integrated flow cell with semiconductor oxide tubing
KR20110061629A (ko) 2008-09-30 2011-06-09 아보트 러보러터리즈 개선된 항체 라이브러리
US20100090341A1 (en) 2008-10-14 2010-04-15 Molecular Imprints, Inc. Nano-patterned active layers formed by nano-imprint lithography
US20100301398A1 (en) * 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010053443A1 (en) * 2008-11-06 2010-05-14 Agency For Science, Technology And Research Apparatus for biopolymer synthesis
US8357489B2 (en) 2008-11-13 2013-01-22 The Board Of Trustees Of The Leland Stanford Junior University Methods for detecting hepatocellular carcinoma
US20110229471A1 (en) 2008-11-26 2011-09-22 Cedars-Sinai Medical Center Methods of determining responsiveness to anti-tnf alpha therapy in inflammatory bowel disease
WO2010064146A2 (en) 2008-12-02 2010-06-10 Chiralgen, Ltd. Method for the synthesis of phosphorus atom modified nucleic acids
US8963262B2 (en) 2009-08-07 2015-02-24 Massachusettes Institute Of Technology Method and apparatus for forming MEMS device
JO3382B1 (ar) 2008-12-23 2019-03-13 Amgen Inc أجسام مضادة ترتبط مع مستقبل cgrp بشري
TW201104253A (en) 2008-12-31 2011-02-01 Nat Health Research Institutes Microarray chip and method of fabricating for the same
WO2010089412A1 (en) 2009-02-09 2010-08-12 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit Und Umwelt (Gmbh) Repertoire of allo-restricted peptide-specific t cell receptor sequences and use thereof
WO2010094772A1 (en) * 2009-02-20 2010-08-26 Febit Holding Gmbh Synthesis of sequence-verified nucleic acids
US8569046B2 (en) 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels
DK2406399T3 (en) 2009-03-09 2018-03-12 Bioatla Llc MIRAC PROTEINS
US8709717B2 (en) * 2009-04-03 2014-04-29 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
US7862716B2 (en) 2009-04-13 2011-01-04 Sielc Technologies Corporation HPLC schematic with integrated sample cleaning system
WO2010124734A1 (en) 2009-04-29 2010-11-04 Telecom Italia S.P.A. Method and apparatus for depositing a biological fluid onto a substrate
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US20100292102A1 (en) 2009-05-14 2010-11-18 Ali Nouri System and Method For Preventing Synthesis of Dangerous Biological Sequences
US20100300882A1 (en) 2009-05-26 2010-12-02 General Electric Company Devices and methods for in-line sample preparation of materials
WO2010141249A2 (en) 2009-06-02 2010-12-09 Merck Sharp & Dohme Corp. Generation, characterization and uses thereof of anti-notch3 antibodies
ES2532891T3 (es) 2009-06-02 2015-04-01 The Regents Of The University Of California Descubrimiento de virus mediante secuenciación y ensamblaje de ARNip, miARN, ARNpi derivados de virus
TWM370169U (en) 2009-06-10 2009-12-01 Wistron Corp Push button component with illumination structure and electronic device
US8309710B2 (en) 2009-06-29 2012-11-13 Agilent Technologies, Inc. Use of N-alkyl imidazole for sulfurization of oligonucleotides with an acetyl disulfide
US8642755B2 (en) 2009-06-30 2014-02-04 Agilent Technologies, Inc. Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
US8329208B2 (en) 2009-07-28 2012-12-11 Methylation Sciences International Srl Pharmacokinetics of S-adenosylmethionine formulations
CA2769302C (en) 2009-07-30 2018-10-16 F. Hoffmann-La Roche Ag A set of oligonucleotide probes as well as methods and uses related thereto
WO2011020529A2 (en) 2009-08-19 2011-02-24 Merck Patent Gmbh Antibodies for the detection of integrin complexes in ffpe material
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US8476598B1 (en) 2009-08-31 2013-07-02 Sionyx, Inc. Electromagnetic radiation imaging devices and associated methods
US20110082055A1 (en) 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
US20120184724A1 (en) 2009-09-22 2012-07-19 Agilent Technologies, Inc. Protected monomers and methods of deprotection for rna synthesis
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
US8975019B2 (en) 2009-10-19 2015-03-10 University Of Massachusetts Deducing exon connectivity by RNA-templated DNA ligation/sequencing
US8389689B2 (en) 2009-10-28 2013-03-05 Janssen Biotech, Inc. Anti-GLP-1R antibodies and their uses
US20120315670A1 (en) 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US20110114549A1 (en) 2009-11-13 2011-05-19 Agilent Technolgies, Inc. Microfluidic device comprising separation columns
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US8500979B2 (en) 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US9758817B2 (en) 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
KR101230350B1 (ko) 2010-01-27 2013-02-06 주식회사 엘지화학 구조적 안정성이 우수한 전지팩
US20120027786A1 (en) 2010-02-23 2012-02-02 Massachusetts Institute Of Technology Genetically programmable pathogen sense and destroy
GB201003036D0 (en) 2010-02-23 2010-04-07 Fermentas Uab Restriction endonucleases and their applications
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2011112713A2 (en) 2010-03-09 2011-09-15 Toxcure, Inc. Microneedle nasal delivery device
WO2011125054A2 (en) 2010-04-09 2011-10-13 The Catholic University Of America Protein and nucleic acid delivery vehicles, components and mechanisms thereof
WO2011130223A1 (en) 2010-04-12 2011-10-20 Mayo Foundation For Medical Education And Research System and method for alleviating freezing gait and gait hypokinesia in users with extrapyramidal disorders
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
DE102010027122A1 (de) * 2010-07-14 2012-01-19 Airbus Operations Gmbh Aufenthaltsmodul mit separatem Privatbereich
MX2013000989A (es) 2010-07-28 2013-03-05 Immunocore Ltd Receptores de celulas t.
CN110878345A (zh) 2010-09-21 2020-03-13 安捷伦科技有限公司 通过分子计数提高等位基因调用的置信度
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012045001A2 (en) 2010-09-30 2012-04-05 Vanderbilt University Influenza virus antibodies and immunogens and uses therefor
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
EP2630264A4 (en) * 2010-10-22 2014-04-02 Harvard College Orthogonal amplification and assembly of nucleic acid sequences
WO2012061832A1 (en) 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2652148B1 (en) 2010-12-17 2016-11-30 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
WO2012092260A1 (en) * 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular dna
ES2899186T3 (es) 2010-12-31 2022-03-10 Bioatla Inc Humanización rápida de anticuerpos
CN103597068A (zh) 2011-03-30 2014-02-19 独立行政法人国立长寿医疗研究中心 膜分选培养器、膜分选培养试剂盒、和使用其的干细胞分选方法、以及分离膜
US10131903B2 (en) 2011-04-01 2018-11-20 The Regents Of The University Of California Microfluidic platform for synthetic biology applications
US9384920B1 (en) 2011-04-04 2016-07-05 Eric J. Bakulich Locking knob
US20140357497A1 (en) 2011-04-27 2014-12-04 Kun Zhang Designing padlock probes for targeted genomic sequencing
US8722585B2 (en) 2011-05-08 2014-05-13 Yan Wang Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
SG194745A1 (en) 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
AU2012267220B2 (en) 2011-06-10 2016-04-21 Bright Devices Group Pty Ltd Freezing of gait cue apparatus
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
WO2012175643A2 (en) 2011-06-21 2012-12-27 Vib Vzw Binding domains directed against gpcr:g protein complexes and uses derived thereof
US9487824B2 (en) 2011-06-28 2016-11-08 Igor Kutyavin Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US20130045483A1 (en) 2011-07-01 2013-02-21 Whitehead Institute For Biomedical Research Yeast cells expressing amyloid beta and uses therefor
US9139874B2 (en) 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
WO2013010062A2 (en) 2011-07-14 2013-01-17 Life Technologies Corporation Nucleic acid complexity reduction
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
US20150203839A1 (en) 2011-08-26 2015-07-23 Gen9, Inc. Compositions and Methods for High Fidelity Assembly of Nucleic Acids
US20150120265A1 (en) 2011-09-01 2015-04-30 Genome Compiler Corporation System for polynucleotide construct design, visualization and transactions to manufacture the same
WO2013036668A1 (en) 2011-09-06 2013-03-14 Gen-Probe Incorporated Circularized templates for sequencing
US8840981B2 (en) * 2011-09-09 2014-09-23 Eastman Kodak Company Microfluidic device with multilayer coating
EP2768607B1 (en) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell plate for high efficiency, small volume nucleic acid synthesis
EP2766838A2 (en) 2011-10-11 2014-08-20 Life Technologies Corporation Systems and methods for analysis and interpretation of nucleic acid sequence data
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US8987174B2 (en) 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
US8815782B2 (en) 2011-11-11 2014-08-26 Agilent Technologies, Inc. Use of DNAzymes for analysis of an RNA sample
US20130137173A1 (en) 2011-11-30 2013-05-30 Feng Zhang Nucleotide-specific recognition sequences for designer tal effectors
US8450107B1 (en) 2011-11-30 2013-05-28 The Broad Institute Inc. Nucleotide-specific recognition sequences for designer TAL effectors
EP2599785A1 (en) 2011-11-30 2013-06-05 Agilent Technologies, Inc. Novel methods for the synthesis and purification of oligomers
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
WO2013093693A1 (en) 2011-12-22 2013-06-27 Rinat Neuroscience Corp. Staphylococcus aureus specific antibodies and uses thereof
EP3495499A1 (en) 2011-12-30 2019-06-12 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
WO2013116771A1 (en) 2012-02-01 2013-08-08 Synthetic Genomics, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
WO2013134881A1 (en) 2012-03-14 2013-09-19 Innovative Targeting Solutions Inc. Generating targeted sequence diversity in fusion proteins
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
JP6238417B2 (ja) 2012-03-28 2017-11-29 ケーシーアイ ライセンシング インコーポレイテッド 電子構成部分と臨床構成部分の分離を促進する減圧システム、ドレッシング、および方法
US9732384B2 (en) 2012-04-02 2017-08-15 Lux Bio Group, Inc. Apparatus and method for molecular separation, purification, and sensing
EP2836821B1 (en) 2012-04-10 2018-09-26 The Trustees of Princeton University Ultra-sensitive sensor
US20150353921A9 (en) 2012-04-16 2015-12-10 Jingdong Tian Method of on-chip nucleic acid molecule synthesis
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130281308A1 (en) 2012-04-24 2013-10-24 Gen9, Inc. Methods for sorting nucleic acids and preparative in vitro cloning
WO2015066174A1 (en) 2013-10-29 2015-05-07 Longhorn Vaccines And Diagnostics, Llc Next generation genomic sequencing methods
ES2749200T3 (es) 2012-05-10 2020-03-19 Bioatla Llc Anticuerpos monoclonales multiespecíficos
EP4148142B1 (en) 2012-05-21 2025-08-13 The Scripps Research Institute Methods of sample preparation
US10308979B2 (en) 2012-06-01 2019-06-04 Agilent Technologies, Inc. Target enrichment and labeling for multi-kilobase DNA
RU2014152796A (ru) 2012-06-01 2016-07-27 Юропиан Молекьюла Байолоджи Лэборетори Накопитель цифровой информации большой емкости в днк
US9102936B2 (en) 2012-06-11 2015-08-11 Agilent Technologies, Inc. Method of adaptor-dimer subtraction using a CRISPR CAS6 protein
CA2877823A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
DK2872629T3 (da) 2012-07-03 2019-12-09 Integrated Dna Tech Inc Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
US20140038240A1 (en) 2012-07-10 2014-02-06 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
WO2014012071A1 (en) 2012-07-12 2014-01-16 Massachusetts Institute Of Technology Methods and apparatus for assembly
JP6239813B2 (ja) 2012-07-18 2017-11-29 株式会社Ｓｃｒｅｅｎセミコンダクターソリューションズ 基板処理装置および基板処理方法
US9384320B2 (en) 2012-07-19 2016-07-05 President And Fellows Of Harvard College Methods of storing information using nucleic acids
HK1211039A1 (en) 2012-07-27 2016-05-13 The Board Of Trustees Of The University Of Illinois Engineering t-cell receptors
WO2014021938A1 (en) * 2012-08-02 2014-02-06 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization
SG11201500724QA (en) 2012-08-16 2015-02-27 Synthetic Genomics Inc Digital to biological converter
JP6590694B2 (ja) 2012-08-31 2019-10-16 ザ スクリプス リサーチ インスティテュート 真核生物細胞の調節因子に関する方法及び組成物
US9328376B2 (en) 2012-09-05 2016-05-03 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
US9133510B2 (en) 2012-10-15 2015-09-15 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
KR20140048733A (ko) 2012-10-16 2014-04-24 삼성전자주식회사 다중 웰 플레이트 및 상기 다중 웰 플레이트를 이용한 표적 물질 분석 방법
US9410173B2 (en) 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
EP2928500B1 (en) 2012-12-04 2019-03-06 Phosphorex Inc. Microparticles and nanoparticles having negative surface charges
EP2929027B1 (en) 2012-12-06 2018-02-21 Agilent Technologies, Inc. Molecular fabrication
WO2014088694A1 (en) 2012-12-06 2014-06-12 Agilent Technologies, Inc. Restriction enzyme-free target enrichment
WO2014092886A2 (en) 2012-12-10 2014-06-19 Agilent Technologies, Inc. Pairing code directed assembly
SG11201504334WA (en) 2012-12-10 2015-07-30 Resolution Bioscience Inc Methods for targeted genomic analysis
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
US20160010045A1 (en) 2013-02-28 2016-01-14 Nanyang Technological University Method of manufacturing a device for supporting biological material growth and device therefrom
US9580746B2 (en) 2013-03-05 2017-02-28 Agilent Technologies, Inc. Synthesis of long fish probes
CN105026577B (zh) 2013-03-05 2020-01-03 安捷伦科技有限公司 通过序列捕获检测基因组重排
US10053719B2 (en) 2013-03-13 2018-08-21 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
EP2971034B1 (en) 2013-03-13 2020-12-02 Gen9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
IL292498A (en) 2013-03-15 2022-06-01 Gen9 Inc Compositions and methods for multiple synthesis of nucleic acids
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
US20140328849A1 (en) 2013-03-15 2014-11-06 Genentech, Inc. Anti-crth2 antibodies and methods of use
EP3795696B1 (en) 2013-03-15 2023-04-26 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US20140274741A1 (en) 2013-03-15 2014-09-18 The Translational Genomics Research Institute Methods to capture and sequence large fragments of dna and diagnostic methods for neuromuscular disease
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US20150293102A1 (en) 2013-04-13 2015-10-15 Jung-Uk Shim Detecting low-abundant analyte in microfluidic droplets
ITRM20130278A1 (it) 2013-05-10 2014-11-11 Consiglio Nazionale Ricerche Procedimento di fabbricazione di film autoassemblati di copolimeri a blocchi
EP3015477B1 (en) 2013-06-26 2021-08-18 XLifeSc, Ltd. High-stability t-cell receptor and preparation method and application thereof
US20150010953A1 (en) 2013-07-03 2015-01-08 Agilent Technologies, Inc. Method for producing a population of oligonucleotides that has reduced synthesis errors
KR20150005062A (ko) 2013-07-04 2015-01-14 삼성전자주식회사 미니-코어를 사용하는 프로세서
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
US20160168564A1 (en) 2013-07-30 2016-06-16 Gen9, Inc. Methods for the Production of Long Length Clonal Sequence Verified Nucleic Acid Constructs
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
US20150044648A1 (en) 2013-08-07 2015-02-12 Nike, Inc. Activity recognition with activity reminders
CN104371019B (zh) 2013-08-13 2019-09-10 鸿运华宁（杭州）生物医药有限公司 一种能与glp-1r特异性结合的抗体及其与glp-1的融合蛋白质
GB201314721D0 (en) 2013-08-16 2013-10-02 Almagen Ltd A method of selectively masking one or more sites on a surface and a method of synthesising an array of molecules
WO2015023976A2 (en) * 2013-08-16 2015-02-19 Memorial Sloan-Kettering Cancer Center Selective grp94 inhibitors and uses thereof
WO2015031689A1 (en) 2013-08-30 2015-03-05 Personalis, Inc. Methods and systems for genomic analysis
CA2924186C (en) 2013-09-14 2023-03-28 Chemgenes Corporation Highly efficient synthesis of long rna using reverse direction approach
WO2015040075A1 (en) 2013-09-18 2015-03-26 Genome Research Limited Genomic screening methods using rna-guided endonucleases
US9311311B2 (en) 2013-09-27 2016-04-12 International Business Machines Corporation Archival management of database logs
US9422325B2 (en) 2013-10-04 2016-08-23 Trustees Of Tufts College Glycosylation reactions using phenyl(trifluoroethyl)iodonium salts
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
WO2015063765A1 (en) 2013-10-29 2015-05-07 Milbat - Giving Quality To Life Walker-assist device
WO2015081142A1 (en) 2013-11-26 2015-06-04 Xenco Medical, Llc Lock and release implant delivery system
CN105934541B (zh) 2013-11-27 2019-07-12 Gen9股份有限公司 核酸文库及其制造方法
MX388731B (es) 2013-12-04 2025-03-20 Chugai Pharmaceutical Co Ltd Moleculas de union al antigeno, cuya actividad de union al antigeno varia de acuerdo con la concentracion de los compuestos y bibliotecas de dichas moleculas.
EP3077419B1 (en) 2013-12-04 2019-08-14 Innovative Targeting Solutions Inc. Peptide-grafted antibodies
CA2945628A1 (en) 2013-12-09 2015-06-18 Integrated Dna Technologies, Inc. Long nuceic acid sequences containing variable regions
EP3647324A1 (en) 2013-12-17 2020-05-06 F. Hoffmann-La Roche AG Methods of treating cancers using pd-1 axis binding antagonists and taxanes
GB2521387B (en) 2013-12-18 2020-05-27 Ge Healthcare Uk Ltd Oligonucleotide data storage on solid supports
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US10287627B2 (en) 2014-02-08 2019-05-14 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking PCR
US10208338B2 (en) 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
EP3590958A1 (en) 2014-03-14 2020-01-08 Immunocore Limited Tcr libraries
WO2015195178A2 (en) 2014-03-27 2015-12-23 Canon U.S. Life Sciences, Inc. Integration of ex situ fabricated porous polymer monoliths into fluidic chips
US10190161B2 (en) 2014-04-03 2019-01-29 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanowire detectors
WO2015160004A1 (ko) 2014-04-15 2015-10-22 볼보 컨스트럭션 이큅먼트 에이비 건설기계의 엔진, 유압펌프의 제어장치 및 그 제어방법
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
CN106536734B (zh) 2014-05-16 2020-12-22 Illumina公司 核酸合成技术
US20150361422A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
US20150361423A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US10472620B2 (en) 2014-07-01 2019-11-12 General Electric Company Method, substrate and device for separating nucleic acids
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
US11254933B2 (en) 2014-07-14 2022-02-22 The Regents Of The University Of California CRISPR/Cas transcriptional modulation
EP3169781B1 (en) 2014-07-15 2020-04-08 Life Technologies Corporation Compositions and methods for nucleic acid assembly
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
EP3913066A1 (en) 2014-09-09 2021-11-24 Igenomx International Genomics Corporation Compositions for rapid nucleic acid library preparation
US20170247756A1 (en) 2014-10-03 2017-08-31 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
CN113930455A (zh) 2014-10-09 2022-01-14 生命技术公司 Crispr寡核苷酸和基因剪辑
EP3204518B1 (en) 2014-10-10 2019-07-10 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
WO2016059610A1 (en) 2014-10-18 2016-04-21 Malik Girik A biomolecule based data storage system
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
US9890417B2 (en) 2014-11-03 2018-02-13 Agilent Technologies, Inc. Signal amplification of fluorescence in situ hybridization
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
CN104562213A (zh) 2014-12-26 2015-04-29 北京诺禾致源生物信息科技有限公司 扩增子文库及其构建方法
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9834774B2 (en) 2015-02-11 2017-12-05 Agilent Technologies, Inc. Methods and compositions for rapid seamless DNA assembly
WO2016130868A2 (en) 2015-02-13 2016-08-18 Vaccine Research Institute Of San Diego Materials and methods to analyze rna isoforms in transcriptomes
CN104734848A (zh) 2015-03-02 2015-06-24 郑州轻工业学院 基于重组dna技术对信息进行加密与隐藏的方法及应用
WO2016161244A2 (en) 2015-04-01 2016-10-06 The Scripps Research Institute Methods and compositions related to gpcr agonist polypeptides
EP3280723B1 (en) 2015-04-08 2021-01-06 Polyphor AG Backbone-cyclized peptidomimetics
WO2016164779A1 (en) 2015-04-10 2016-10-13 University Of Washington Integrated system for nucleic acid-based storage of digital data
US10125198B2 (en) 2015-04-17 2018-11-13 Distributed Bio, Inc. Method for mass humanization of non-human antibodies
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3288973B1 (en) 2015-04-30 2021-10-20 AbCheck s.r.o. Method for mass humanization of rabbit antibodies
WO2016183100A1 (en) 2015-05-11 2016-11-17 Twist Bioscience Corporation Compositions and methods for nucleic acid amplification
EP3322812B1 (en) 2015-07-13 2022-05-18 President and Fellows of Harvard College Methods for retrievable information storage using nucleic acids
GB201513113D0 (en) 2015-07-24 2015-09-09 Genome Res Ltd Nasal sampling methods
EA201890763A1 (ru) 2015-09-18 2018-08-31 Твист Байосайенс Корпорейшн Библиотеки вариантных олигонуклеиновых кислот и их синтез
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
US20180320166A1 (en) 2015-10-01 2018-11-08 University Of Washington Multiplex pairwise assembly of dna oligonucleotides
US20170141793A1 (en) 2015-11-13 2017-05-18 Microsoft Technology Licensing, Llc Error correction for nucleotide data stores
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
PL3387152T3 (pl) 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
AU2017205706B2 (en) 2016-01-08 2022-10-20 Maxion Therapeutics Limited Binding members with altered diversity scaffold domains
GB201604492D0 (en) 2016-03-16 2016-04-27 Immatics Biotechnologies Gmbh Transfected t-cells and t-cell receptors for use in immunotherapy against cancers
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
WO2017214574A1 (en) 2016-06-10 2017-12-14 Twist Bioscience Corporation Systems and methods for automated annotation and screening of biological sequences
CN110088281A (zh) 2016-08-03 2019-08-02 特韦斯特生物科学公司 用于多核苷酸合成的纹理化表面
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
JP7160482B2 (ja) 2016-09-02 2022-10-25 レンティジェン・テクノロジー・インコーポレイテッド Ｄｕｏｃａｒを用いてがんを処置するための組成物および方法
EP3516528B1 (en) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nucleic acid based data storage
KR102569164B1 (ko) 2016-11-18 2023-08-21 트위스트 바이오사이언스 코포레이션 조절된 화학량론을 갖는 폴리뉴클레오티드 라이브러리 및 이의 합성
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
FI3558369T3 (fi) 2016-12-21 2025-05-02 Cephalon Llc Spesifisesti ihmisen il-15:een sitoutuvia vasta-aineita ja niiden käyttötapoja
JP2020508661A (ja) 2017-02-22 2020-03-26 ツイスト バイオサイエンス コーポレーション 核酸ベースのデータ保存
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018170164A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
UA125614C2 (uk) 2017-03-23 2022-05-04 Кьюбіотікс Пті Лтд Комбінована терапія для лікування або профілактики пухлин
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
ES2929837T3 (es) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
CN110832087B (zh) 2017-05-08 2025-03-18 伊鲁米那股份有限公司 用于多核苷酸样品的索引的通用短衔接子
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11201912057RA (en) 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
JP7015388B2 (ja) 2017-07-18 2022-02-02 インベスティガシオネス・フォレスタレス・ビオフォレスト・エス・アー 電気膜プロセスにおける非対称極性反転のための方法とデバイス
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
KR102637566B1 (ko) 2017-10-20 2024-02-16 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US20200299684A1 (en) 2017-10-27 2020-09-24 Twist Bioscience Corporation Systems and methods for polynucleotide scoring
US11427867B2 (en) 2017-11-29 2022-08-30 Xgenomes Corp. Sequencing by emergence
CN112041438B (zh) 2018-01-04 2025-05-23 特韦斯特生物科学公司 基于dna的数字信息存储
US10722916B2 (en) 2018-01-19 2020-07-28 Caulk Garbage Can LLC Caulk gun attachment for wiping excess caulk
CN118957038A (zh) 2018-05-18 2024-11-15 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
WO2020001783A1 (en) 2018-06-29 2020-01-02 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
US10963953B2 (en) 2018-10-10 2021-03-30 Alliance Inspection Management, LLC Reserve management for continuous bidding portal
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
EP3938505A4 (en) 2019-02-25 2022-11-30 Twist Bioscience Corporation COMPOSITIONS AND METHODS FOR SEQUENCING THE NEXT GENERATION
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
US20220243195A1 (en) 2019-06-21 2022-08-04 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2020257612A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4004232A4 (en) 2019-07-22 2023-08-09 Igenomx International Genomics Corporation METHODS AND COMPOSITIONS FOR HIGH THROUGHPUT SAMPLE PREPARATION USING A UNIQUE DUAL INDEXING
WO2021046655A1 (en) 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
WO2021061829A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
WO2021119193A2 (en) 2019-12-09 2021-06-17 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
WO2021222316A2 (en) 2020-04-27 2021-11-04 Twist Bioscience Corporation Variant nucleic acid libraries for coronavirus
EP4179001A4 (en) 2020-07-07 2024-07-10 Twist Bioscience Corporation DEVICES AND METHODS FOR LIGHT ORIENTATION POLYMER SYNTHESIS
US20220106586A1 (en) 2020-08-25 2022-04-07 Twist Bioscience Corporation Compositions and methods for library sequencing
JP2023539245A (ja) 2020-08-26 2023-09-13 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１ｒ変異体に関する方法および組成物
WO2022047076A1 (en) 2020-08-28 2022-03-03 Twist Bioscience Corporation Devices and methods for synthesis
CA3194398A1 (en) 2020-10-05 2022-04-14 Twist Bioscience Corporation Hybridization methods and reagents
US11970697B2 (en) 2020-10-19 2024-04-30 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
WO2022087293A1 (en) 2020-10-22 2022-04-28 Twist Bioscience Corporation Methods and systems for detecting coronavirus
WO2022093811A1 (en) 2020-10-26 2022-05-05 Twist Bioscience Corporation Libraries for next generation sequencing
WO2022098662A2 (en) 2020-11-03 2022-05-12 Twist Bioscience Corporation Methods and compositions relating to chemokine receptor variants
CA3206018A1 (en) 2021-01-21 2022-07-28 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
WO2022178137A1 (en) 2021-02-19 2022-08-25 Twist Bioscience Corporation Libraries for identification of genomic variants

Also Published As

Publication number Publication date
US9409139B2 (en) 2016-08-09
TW201843302A (zh) 2018-12-16
TWI646230B (zh) 2019-01-01
US11185837B2 (en) 2021-11-30
SG11201600853UA (en) 2016-03-30
KR102423377B1 (ko) 2022-07-25
US11452980B2 (en) 2022-09-27
KR102351838B1 (ko) 2022-01-18
EA201690081A1 (ru) 2016-10-31
JP2023093506A (ja) 2023-07-04
JP2021118690A (ja) 2021-08-12
US10618024B2 (en) 2020-04-14
EP3722442B1 (en) 2023-04-05
US20190314783A1 (en) 2019-10-17
US11559778B2 (en) 2023-01-24
US20160096160A1 (en) 2016-04-07
TW202102674A (zh) 2021-01-16
US20180326388A1 (en) 2018-11-15
US20170327819A1 (en) 2017-11-16
US20160089651A1 (en) 2016-03-31
EP3030682A2 (en) 2016-06-15
KR20160041046A (ko) 2016-04-15
US10384188B2 (en) 2019-08-20
US20190366294A1 (en) 2019-12-05
TW201606149A (zh) 2016-02-16
KR20210102999A (ko) 2021-08-20
US9839894B2 (en) 2017-12-12
US20180264428A1 (en) 2018-09-20
EA034459B1 (ru) 2020-02-11
WO2015021080A3 (en) 2015-05-28
US20170095785A1 (en) 2017-04-06
US20160354752A1 (en) 2016-12-08
EP3722442A1 (en) 2020-10-14
US10773232B2 (en) 2020-09-15
US10272410B2 (en) 2019-04-30
US20190366293A1 (en) 2019-12-05
ES2943498T3 (es) 2023-06-13
US20170362589A1 (en) 2017-12-21
TWI805996B (zh) 2023-06-21
US9555388B2 (en) 2017-01-31
KR102291045B1 (ko) 2021-08-19
EP4242321A3 (en) 2023-09-27
ES2815099T3 (es) 2021-03-29
TW202030326A (zh) 2020-08-16
US20230211308A1 (en) 2023-07-06
EP4610368A2 (en) 2025-09-03
EP4610368A3 (en) 2025-11-05
KR20200111278A (ko) 2020-09-28
WO2015021080A2 (en) 2015-02-12
US10632445B2 (en) 2020-04-28
US9833761B2 (en) 2017-12-05
PL3030682T3 (pl) 2020-11-16
EP4242321B1 (en) 2025-06-25
TW202118874A (zh) 2021-05-16
KR102160389B1 (ko) 2020-09-28
KR102207770B1 (ko) 2021-01-26
KR20210008925A (ko) 2021-01-25
US20150038373A1 (en) 2015-02-05
US10583415B2 (en) 2020-03-10
EP3030682A4 (en) 2017-05-31
US20160340672A1 (en) 2016-11-24
GB201502580D0 (en) 2015-04-01
KR20220012401A (ko) 2022-02-03
DK3030682T3 (da) 2020-09-14
CN105637097A (zh) 2016-06-01
US20160090592A1 (en) 2016-03-31
KR20200030130A (ko) 2020-03-19
KR102122632B1 (ko) 2020-06-16
US20200156037A1 (en) 2020-05-21
JP2016527313A (ja) 2016-09-08
US20230086062A1 (en) 2023-03-23
US20180029001A1 (en) 2018-02-01
CA2918258A1 (en) 2015-02-12
EP3030682B1 (en) 2020-06-03
TWI707038B (zh) 2020-10-11
US20160303535A1 (en) 2016-10-20
US20160339409A1 (en) 2016-11-24
TWI695067B (zh) 2020-06-01
EP4242321A2 (en) 2023-09-13
US9403141B2 (en) 2016-08-02
TWI721929B (zh) 2021-03-11
HK1225761A1 (en) 2017-09-15
US10639609B2 (en) 2020-05-05
JP2020022453A (ja) 2020-02-13
CN111593414A (zh) 2020-08-28
US9889423B2 (en) 2018-02-13
GB2533173A (en) 2016-06-15

Similar Documents

Publication Publication Date Title
US20230086062A1 (en) De novo synthesized gene libraries
HK40100569B (en) De novo synthesized gene libraries
HK40100569A (en) De novo synthesized gene libraries
HK1225761B (en) De novo synthesized gene libraries

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160314

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170731

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170801

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170912

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20180614

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180808

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181106

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181116

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20190507

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190909

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20191018

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200107

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200203

R150 Certificate of patent or registration of utility model

Ref document number: 6656733

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250