DK3030682T3 - De novo synthesized gene libraries - Google Patents

De novo synthesized gene libraries Download PDF

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Publication number
DK3030682T3
DK3030682T3 DK14834665.3T DK14834665T DK3030682T3 DK 3030682 T3 DK3030682 T3 DK 3030682T3 DK 14834665 T DK14834665 T DK 14834665T DK 3030682 T3 DK3030682 T3 DK 3030682T3
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Prior art keywords
gene libraries
synthesized gene
novo synthesized
novo
libraries
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DK14834665.3T
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Danish (da)
English (en)
Inventor
William Banyai
Bill James Peck
Pierre Indermuhle
Andres Fernandez
Siyuan Chen
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Twist Bioscience Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00279—Features relating to reactor vessels
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    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00718—Type of compounds synthesised
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DK14834665.3T 2013-08-05 2014-08-05 De novo synthesized gene libraries DK3030682T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361862457P 2013-08-05 2013-08-05
US201361862445P 2013-08-05 2013-08-05
PCT/US2014/049834 WO2015021080A2 (en) 2013-08-05 2014-08-05 De novo synthesized gene libraries

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US (20) US9409139B2 (enExample)
EP (4) EP4610368B1 (enExample)
JP (4) JP6656733B2 (enExample)
KR (6) KR102122632B1 (enExample)
CN (2) CN105637097A (enExample)
CA (1) CA2918258A1 (enExample)
DK (1) DK3030682T3 (enExample)
EA (1) EA034459B1 (enExample)
ES (2) ES2815099T3 (enExample)
GB (1) GB2533173A (enExample)
PL (1) PL3030682T3 (enExample)
SG (1) SG11201600853UA (enExample)
TW (5) TWI707038B (enExample)
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Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3564395B1 (en) 2010-12-30 2025-06-25 Foundation Medicine, Inc. Optimization of multigene analysis of tumor samples
EP4610368B1 (en) 2013-08-05 2026-02-04 Twist Bioscience Corporation De novo synthesized gene libraries
CN110283888B (zh) 2013-08-19 2021-06-22 卓异生物公司 用于单分子检测的测定及其应用
WO2016044227A1 (en) * 2014-09-15 2016-03-24 Abvitro, Inc. High-throughput nucleotide library sequencing
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
WO2016090273A1 (en) 2014-12-05 2016-06-09 Foundation Medicine, Inc. Multigene analysis of tumor samples
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CN107735497B (zh) * 2015-02-18 2021-08-20 卓异生物公司 用于单分子检测的测定及其应用
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
AU2016323062A1 (en) * 2015-09-14 2018-04-12 Essenlix Corp. Device and system for analyzing a sample, particularly blood, as well as methods of using the same
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods for modulating protein and cellular activity and method for nucleic acid synthesis
CN108698012A (zh) * 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
WO2017070549A1 (en) * 2015-10-21 2017-04-27 Genia Technologies, Inc. Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
EP3871695A1 (en) 2015-12-07 2021-09-01 Arc Bio, LLC Methods and compositions for the making and using of guide nucleic acids
JP2019520421A (ja) * 2016-04-01 2019-07-18 ヘルステル・インコーポレイテッドＨｅａｌｔｈｔｅｌｌ Ｉｎｃ． 治療抗体の特徴付けのためのアレイに基づくペプチドライブラリー
CN109564769A (zh) * 2016-06-10 2019-04-02 特韦斯特生物科学公司 用于自动注释和筛选生物序列的系统和方法
JP7283727B2 (ja) 2016-06-16 2023-05-30 ヘイスタック サイエンシィズ コーポレーション コードプローブ分子のコンビナトリアル合成が指令されかつ記録されたオリゴヌクレオチド
CN110088281A (zh) * 2016-08-03 2019-08-02 特韦斯特生物科学公司 用于多核苷酸合成的纹理化表面
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
CN110072870A (zh) 2016-11-09 2019-07-30 健康之语公司 预组装的、受保护的、化学稳定的化学选择性接头
EP3538591A4 (en) 2016-11-09 2020-07-29 Healthtell Inc. ADJUSTABLE AMINE DENSITY COATINGS
JP2020504709A (ja) * 2016-11-18 2020-02-13 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
MX2019006753A (es) 2016-12-22 2020-01-30 Illumina Inc Paquete de celda de flujo y metodo para fabricar el mismo.
EP4556433A3 (en) * 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
CA3056386A1 (en) * 2017-03-15 2018-09-20 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation OLIGONUCLEOTID-DRIVEN COMBINATORY SYNTHESIS VERIFICATION MOLECULES AND ASSOCIATED MANUFACTURING AND USE PROCESSES
WO2018217689A1 (en) 2017-05-22 2018-11-29 The Charles Stark Draper Laboratory, Inc. Modified template-independent dna polymerase
US10538808B2 (en) * 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
AU2018279112B2 (en) * 2017-06-07 2024-11-07 Arc Bio, Llc Creation and use of guide nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566209B (zh) * 2017-06-12 2024-08-30 特韦斯特生物科学公司 无缝核酸装配方法
US20190076814A1 (en) 2017-09-11 2019-03-14 Synthego Corporation Biopolymer synthesis system and method
EP3681906A4 (en) 2017-09-11 2021-06-09 Twist Bioscience Corporation GPCR-BINDING PROTEINS AND THEIR SYNTHESIS
EP3681637A4 (en) * 2017-09-11 2021-06-02 Synthego Corporation SYSTEM AND PROCEDURE FOR BIOPOLYMER SYNTHESIS
CN111295444B (zh) * 2017-09-25 2024-08-09 哈斯达克科学公司 用于寡核苷酸指导的组合化学的多项式编码
US11084037B2 (en) * 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
WO2019074524A1 (en) 2017-10-13 2019-04-18 Hewlett-Packard Development Company, L.P. SEPARATION OF A LIQUID IN SAMPLE
KR102889470B1 (ko) * 2017-10-20 2025-11-21 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
CA3088911A1 (en) * 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based storage device and method for synthesizing polynucleotides using the device
CN111919118B (zh) * 2018-04-13 2025-09-05 豪夫迈·罗氏有限公司 用于检测和分析分析物的方法和组合物
US12319712B2 (en) 2018-05-09 2025-06-03 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photoactivated agents
WO2019222612A1 (en) * 2018-05-17 2019-11-21 Georgia Tech Research Corporation Devices, systems, and methods of electronic modulation of polymerase for dna synthesis
WO2019222650A1 (en) * 2018-05-17 2019-11-21 The Charles Stark Draper Laboratory, Inc. Apparatus for high density information storage in molecular chains
WO2019222706A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202011696TA (en) 2018-06-11 2020-12-30 Foundation Medicine Inc Compositions and methods for evaluating genomic alterations
US11854669B1 (en) 2018-07-30 2023-12-26 National Technology & Engineering Solutions Of Sandia, Llc Synthetic nucleic acids for information storage and transmission
CN109136331A (zh) * 2018-08-22 2019-01-04 中山大学 一种高灵敏度高特异性错配核酸序列的设计方法
JP7322365B2 (ja) * 2018-09-06 2023-08-08 株式会社レゾナック サセプタ及び化学気相成長装置
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
WO2020102583A1 (en) * 2018-11-14 2020-05-22 Synthomics, Inc. Methods, devices, and systems for biopolymer synthesis
KR20210098491A (ko) * 2018-11-30 2021-08-10 진인포섹, 아이엔씨. 무작위 올리고뉴클레오티드를 생성하고 그 서열을 결정하는 방법
WO2020123311A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
TWI845600B (zh) 2019-01-24 2024-06-21 美商普羅米修斯生物科學股份有限公司 Gpr35調節劑
EP4407044A1 (en) * 2019-01-28 2024-07-31 BGI Shenzhen Sequencing chip and manufacturing method therefor
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
KR102187795B1 (ko) * 2019-04-30 2020-12-08 (주)디엑솜 디옥시우리딘을 이용한 차세대 염기서열 분석을 위한 라이브러리 제조 방법
SG11202012812PA (en) 2019-05-31 2021-01-28 Illumina Inc Flow cell with selective deposition or activation of nucleotides
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN110349628B (zh) * 2019-06-27 2021-06-15 广东药科大学 一种蛋白质磷酸化位点识别方法、系统、装置及存储介质
EP4017994A1 (en) * 2019-08-22 2022-06-29 New England Biolabs, Inc. Cleavage of single stranded dna having a modified nucleotide
CN110531099B (zh) * 2019-08-23 2024-04-12 湖南莱博赛医用机器人有限公司 一种分体式玻片盒及细胞分析设备
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CZ2019720A3 (cs) * 2019-11-23 2021-01-13 Grade Medical s.r.o. Zařízení pro přípravu funkcionalizovaných substrátů
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
CN111072729B (zh) * 2019-12-13 2023-06-13 天津大学 一种寡核苷酸合成用固相载体装置及其选择性修饰方法
WO2021137844A1 (en) * 2019-12-30 2021-07-08 Dna Syn Tech Inc. Apparatus and method for preparing nucleic acid sequences using enzyme
WO2021142133A1 (en) * 2020-01-07 2021-07-15 Elegen Corporation Dna assembly in microfluidics device having integrated solid-phase columns
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
EP4142739A2 (en) 2020-04-27 2023-03-08 Twist Bioscience Corporation Variant nucleic acid libraries for coronavirus
WO2021231483A1 (en) 2020-05-12 2021-11-18 Illumina, Inc. Thermostable terminal deoxynucleotidyl transferase
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
KR20230024399A (ko) * 2020-06-16 2023-02-20 디엔에이 스크립트 효소적 폴리뉴클레오티드 합성을 위한 시스템, 장치 및 키트
CN115836592A (zh) 2020-06-16 2023-03-21 3M创新有限公司 包括金属体的图案化的制品
WO2022019836A1 (en) 2020-07-21 2022-01-27 Illumina Singapore Pte Ltd Base-modified nucleotides as substrates for tdt-based enzymatic nucleic acid
EP3958450A1 (de) * 2020-08-21 2022-02-23 Schneider Electric Industries SAS Linearmotorsystem und betriebsverfahren für ein solches
US20220106586A1 (en) * 2020-08-25 2022-04-07 Twist Bioscience Corporation Compositions and methods for library sequencing
CA3190667A1 (en) 2020-08-26 2022-03-03 Aaron Sato Methods and compositions relating to glp1r variants
CA3190917A1 (en) 2020-08-28 2022-03-03 Andres Fernandez Devices and methods for synthesis
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
CA3204784A1 (en) * 2021-01-13 2022-07-21 Alex Nemiroski Surface structuring with colloidal assembly
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
WO2022177982A1 (en) * 2021-02-18 2022-08-25 Battelle Memorial Institute Nanowell array device for high throughput sample analysis
WO2022177273A1 (ko) * 2021-02-18 2022-08-25 서울대학교산학협력단 핵산 라이브러리의 정제방법
EP4313984A4 (en) * 2021-03-22 2025-05-21 Genscript USA Inc. UNIVERSAL LINKER REAGENTS FOR DNA SYNTHESIS
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
CN113262730B (zh) * 2021-03-29 2022-11-22 上海迪赢生物科技有限公司 一种基于簇式阵列的高通量自动化基因合成装置
CA3214947A1 (en) * 2021-04-09 2022-10-13 Twist Bioscience Corporation Libraries for mutational analysis
EP4323541A1 (en) 2021-04-13 2024-02-21 Elegen Corporation Methods and compositions for cell-free cloning
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
EP4334516A1 (en) * 2021-05-03 2024-03-13 Twist Bioscience Corporation Variant nucleic acid libraries for ion channels
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
US12571024B2 (en) 2021-08-19 2026-03-10 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
US20240379186A1 (en) 2021-09-13 2024-11-14 Oncodna Method to generate personalized neoantigens of a tumor of a patient
EP4147712A1 (en) 2021-09-13 2023-03-15 OncoDNA Method to generate a double-stranded dna pool encoding neoantigens of a tumor of a patient
CN114100714B (zh) * 2021-11-22 2023-01-17 上海睿度光电科技有限公司 一种核酸或多肽高通量合成芯片及其用途
US12325739B2 (en) 2022-01-03 2025-06-10 Twist Bioscience Corporation Bispecific SARS-CoV-2 antibodies and methods of use
US20250084397A1 (en) * 2022-01-04 2025-03-13 Modernatx, Inc. Methods of purifying dna for gene synthesis
GB202205696D0 (en) * 2022-04-19 2022-06-01 Moligo Tech Ab Method for producing double stranded DNA
GB2621159B (en) * 2022-08-04 2025-08-13 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor
CA3263806A1 (en) 2022-08-16 2024-02-22 Aldevron, L.L.C. CELLULAR PROCESS FOR THE PRODUCTION OF SYNTHETIC CIRCULAR NUCLEIC ACID
WO2024047178A1 (en) 2022-09-02 2024-03-07 Eleven Therapeutics Ltd A method for simultaneous synthesis of a plurality of oligonucleotides
WO2024073136A1 (en) * 2022-09-30 2024-04-04 Mary Hitchcock Memorial Hospital, For Itself And On Behalf Of Dartmouth-Hitchcock Clinic Rapid reconstruction of large nucleic acids
EP4364752A1 (en) 2022-11-07 2024-05-08 OncoDNA Improved vaccine
WO2024112803A2 (en) * 2022-11-22 2024-05-30 Yale University Methods and kits for microscopic imaging
WO2024137514A1 (en) * 2022-12-22 2024-06-27 Synthego Corporation Systems and method for automated oligonucleotide synthesis
WO2024141629A1 (en) * 2022-12-31 2024-07-04 Dna Script Heat treatment means for printing device of an enzymatic synthesis apparatus
CN116434843A (zh) * 2023-03-29 2023-07-14 赛纳生物科技(北京)有限公司 一种碱基测序质量评估方法
EP4695287A1 (en) 2023-04-14 2026-02-18 Twist Bioscience Corporation Compositions and methods related to dkk1 binders
WO2025072262A1 (en) * 2023-09-26 2025-04-03 Instance, Inc. Ultra-high capacity dna synthesis to power functionally generative biological design
WO2026044542A1 (zh) * 2024-08-28 2026-03-05 上海交通大学 一种空间转录组芯片及其制备方法与应用

Family Cites Families (946)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1005368A (en) 1909-10-29 1911-10-10 John M Travis Faucet.
US1027241A (en) 1911-07-03 1912-05-21 James Mccutcheon Coleman Dump-car construction.
US3549368A (en) 1968-07-02 1970-12-22 Ibm Process for improving photoresist adhesion
US3920714A (en) 1972-11-16 1975-11-18 Weber Heinrich Process for the production of polymeric hydrocarbons with reactive silyl side groups
GB1550867A (en) 1975-08-04 1979-08-22 Hughes Aircraft Co Positioning method and apparatus for fabricating microcircuit devices
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
EP0090789A1 (en) 1982-03-26 1983-10-05 Monsanto Company Chemical DNA synthesis
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
JPS59224123A (ja) 1983-05-20 1984-12-17 Oki Electric Ind Co Ltd ウエハアライメントマ−ク
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
JPS61141761A (ja) 1984-12-12 1986-06-28 Kanegafuchi Chem Ind Co Ltd 硬化性組成物
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4613398A (en) 1985-06-06 1986-09-23 International Business Machines Corporation Formation of etch-resistant resists through preferential permeation
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4726877A (en) 1986-01-22 1988-02-23 E. I. Du Pont De Nemours And Company Methods of using photosensitive compositions containing microgels
US5763192A (en) 1986-11-20 1998-06-09 Ixsys, Incorporated Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US4808511A (en) 1987-05-19 1989-02-28 International Business Machines Corporation Vapor phase photoresist silylation process
JPH07113774B2 (ja) 1987-05-29 1995-12-06 株式会社日立製作所 パタ−ンの形成方法
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
DE69028725T2 (de) 1989-02-28 1997-03-13 Canon Kk Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5459039A (en) 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US6008031A (en) 1989-05-12 1999-12-28 Duke University Method of analysis and manipulation of DNA utilizing mismatch repair systems
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6919211B1 (en) 1989-06-07 2005-07-19 Affymetrix, Inc. Polypeptide arrays
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US6087482A (en) 1990-07-27 2000-07-11 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
FI87886C (fi) 1990-09-06 1993-03-10 Instrumentarium Oy Faestorgan
EP0738779B1 (en) 1990-09-27 2004-05-19 Invitrogen Corporation Direct cloning of PCR amplified nucleic acids
GB9025236D0 (en) 1990-11-20 1991-01-02 Secr Defence Silicon-on porous-silicon;method of production
EP0834576B1 (en) 1990-12-06 2002-01-16 Affymetrix, Inc. (a Delaware Corporation) Detection of nucleic acid sequences
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
ES2155822T3 (es) 1990-12-06 2001-06-01 Affymetrix Inc Compuestos y su utilizacion en una estrategia de sintesis binaria.
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5137814A (en) 1991-06-14 1992-08-11 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
US5449754A (en) 1991-08-07 1995-09-12 H & N Instruments, Inc. Generation of combinatorial libraries
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7045289B2 (en) 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
DE69233087T2 (de) 1991-11-22 2003-12-24 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Verfahren zur Herstellung von Polymerarrays
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1993020236A1 (en) 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
JP2553322Y2 (ja) 1992-05-11 1997-11-05 サンデン株式会社 飲料抽出装置のフィルタ送り機構
WO1994003637A1 (en) 1992-07-31 1994-02-17 Syntex (Usa) Inc. Method for introducing defined sequences at the 3' end of polynucleotides
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
JP3176444B2 (ja) 1992-10-01 2001-06-18 株式会社リコー 水性インク及びこれを用いた記録方法
DE4241045C1 (de) 1992-12-05 1994-05-26 Bosch Gmbh Robert Verfahren zum anisotropen Ätzen von Silicium
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
US5395753A (en) 1993-02-19 1995-03-07 Theratech, Inc. Method for diagnosing rheumatoid arthritis
DE69433010T2 (de) 1993-04-12 2004-06-09 Northwestern University, Evanston Verfahren zur darstellung von oligonukleotiden
US7135312B2 (en) * 1993-04-15 2006-11-14 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5455239A (en) 1993-08-05 1995-10-03 Merck & Co. Inc. 3-aryl of heteroaryl-7-heteroaralkylamido cephalosporin compounds, compositions and methods of use
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
CN1039623C (zh) 1993-10-22 1998-09-02 中国人民解放军军事医学科学院毒物药物研究所 一种防治运动病综合征的药物组合物及其制备方法
US6893816B1 (en) 1993-10-28 2005-05-17 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
JP3488465B2 (ja) 1993-10-28 2004-01-19 ヒューストン・アドバンスド・リサーチ・センター 結合反応を別々に検出する微細加工フロースルー多孔性装置
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6015880A (en) 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US5824531A (en) 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
SE512382C2 (sv) 1994-04-26 2000-03-06 Ericsson Telefon Ab L M Anordning och förfarande för att placera långsträckta element mot eller invid en yta
EP0706649B1 (en) 1994-04-29 2001-01-03 Perkin-Elmer Corporation Method and apparatus for real time detection of nucleic acid amplification products
US6287850B1 (en) 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
CA2193228A1 (en) 1994-06-23 1996-01-04 Christopher Holmes Photolabile compounds and methods for their use
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5530516A (en) 1994-10-04 1996-06-25 Tamarack Scientific Co., Inc. Large-area projection exposure system
US6613560B1 (en) 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
US6635226B1 (en) 1994-10-19 2003-10-21 Agilent Technologies, Inc. Microanalytical device and use thereof for conducting chemical processes
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
WO1996015861A1 (en) 1994-11-22 1996-05-30 Complex Fluid Systems, Inc. Non-aminic photoresist adhesion promoters for microelectronic applications
US5688642A (en) 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5877280A (en) 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US6446682B1 (en) 1995-06-06 2002-09-10 James P. Viken Auto-loading fluid exchanger and method of use
US5707806A (en) 1995-06-07 1998-01-13 Genzyme Corporation Direct sequence identification of mutations by cleavage- and ligation-associated mutation-specific sequencing
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US5780613A (en) 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
JP2000501615A (ja) 1995-12-15 2000-02-15 アマーシャム・ライフ・サイエンス・インコーポレーテッド 酵素増幅中に生じる変異配列の検出および除去のためのミスマッチ修復系を用いる方法
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
US7432048B2 (en) 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US6090606A (en) 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7527928B2 (en) 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6706875B1 (en) 1996-04-17 2004-03-16 Affyemtrix, Inc. Substrate preparation process
US5869245A (en) 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands
US5863801A (en) 1996-06-14 1999-01-26 Sarnoff Corporation Automated nucleic acid isolation
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US5853993A (en) 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
AU5173598A (en) 1996-11-08 1998-06-10 Ikonos Corporation Method for coating substrates
US5750672A (en) 1996-11-22 1998-05-12 Barrskogen, Inc. Anhydrous amine cleavage of oligonucleotides
EP2374890B1 (en) 1996-11-29 2012-11-14 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
CA2389358C (en) * 1996-12-31 2008-07-15 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
DE69829976T2 (de) 1997-02-12 2006-04-27 Invitrogen Corp., Carlsbad Verfahren zur herstellung von zellen
US5882496A (en) 1997-02-27 1999-03-16 The Regents Of The University Of California Porous silicon structures with high surface area/specific pore size
US6770748B2 (en) 1997-03-07 2004-08-03 Takeshi Imanishi Bicyclonucleoside and oligonucleotide analogue
US6028189A (en) 1997-03-20 2000-02-22 University Of Washington Solvent for oligonucleotide synthesis and methods of use
US6419883B1 (en) 1998-01-16 2002-07-16 University Of Washington Chemical synthesis using solvent microdroplets
IL131978A0 (en) 1997-03-20 2001-03-19 Univ Washington Solvent for biopolymer synthesis solvent microdots and methods of use
JP4245084B2 (ja) 1997-03-21 2009-03-25 ストラタジーン ポリメラーゼ増強因子（ｐｅｆ）抽出物、ｐｅｆタンパク質複合体、単離されたｐｅｆタンパク質、並びに精製及び単離方法
US5922593A (en) 1997-05-23 1999-07-13 Becton, Dickinson And Company Microbiological test panel and method therefor
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
DE69824586T2 (de) 1997-06-26 2005-06-23 PerSeptive Biosystems, Inc., Framingham Probenträger hoher dichte für die analyse biologischer proben
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US6027898A (en) 1997-08-18 2000-02-22 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6136568A (en) 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
DK1015576T3 (da) 1997-09-16 2005-08-29 Egea Biosciences Llc Fremgangsmåde til fuldstændig kemisk syntese og aggregering af gener og genomer
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5976842A (en) 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6408308B1 (en) 1998-01-29 2002-06-18 Incyte Pharmaceuticals, Inc. System and method for generating, analyzing and storing normalized expression datasets from raw expression datasets derived from microarray includes nucleic acid probe sequences
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
US6251588B1 (en) 1998-02-10 2001-06-26 Agilent Technologies, Inc. Method for evaluating oligonucleotide probe sequences
CA2319587C (en) 1998-02-11 2004-09-21 University Of Houston Method and apparatus for chemical and biochemical reactions using photo-generated reagents
US20040035690A1 (en) * 1998-02-11 2004-02-26 The Regents Of The University Of Michigan Method and apparatus for chemical and biochemical reactions using photo-generated reagents
AU746760B2 (en) 1998-02-23 2002-05-02 Wisconsin Alumni Research Foundation Method and apparatus for synthesis of arrays of DNA probes
AU3601599A (en) 1998-03-25 1999-10-18 Ulf Landegren Rolling circle replication of padlock probes
US6284497B1 (en) 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
US7321828B2 (en) 1998-04-13 2008-01-22 Isis Pharmaceuticals, Inc. System of components for preparing oligonucleotides
AU762397B2 (en) 1998-04-13 2003-06-26 Isis Pharmaceuticals, Inc. Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
US6376285B1 (en) 1998-05-28 2002-04-23 Texas Instruments Incorporated Annealed porous silicon with epitaxial layer for SOI
US6274725B1 (en) 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
US6130045A (en) 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
US6251595B1 (en) 1998-06-18 2001-06-26 Agilent Technologies, Inc. Methods and devices for carrying out chemical reactions
ATE313548T1 (de) 1998-06-22 2006-01-15 Affymetrix Inc Reagenz und verfahren zu fester phase synthese
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
US7399844B2 (en) 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US6222030B1 (en) 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6107038A (en) 1998-08-14 2000-08-22 Agilent Technologies Inc. Method of binding a plurality of chemicals on a substrate by electrophoretic self-assembly
CA2341896A1 (en) 1998-08-28 2000-03-09 Febit Ferrarius Biotechnology Gmbh Support for a method for determining an analyte and a method for producing the support
US6258454B1 (en) 1998-09-01 2001-07-10 Agilent Technologies Inc. Functionalization of substrate surfaces with silane mixtures
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6461812B2 (en) 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
AU770993B2 (en) 1998-09-15 2004-03-11 Yale University Molecular cloning using rolling circle amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6399516B1 (en) 1998-10-30 2002-06-04 Massachusetts Institute Of Technology Plasma etch techniques for fabricating silicon structures from a substrate
US6309828B1 (en) 1998-11-18 2001-10-30 Agilent Technologies, Inc. Method and apparatus for fabricating replicate arrays of nucleic acid molecules
AU2395900A (en) 1998-12-31 2000-07-31 Gene Logic, Inc. Assay device comprising mixed probes
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
AU2415200A (en) 1999-01-18 2000-08-01 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
JP2002534966A (ja) 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド オリゴヌクレオチド媒介核酸組換え
US6251685B1 (en) 1999-02-18 2001-06-26 Agilent Technologies, Inc. Readout method for molecular biological electronically addressable arrays
DE50015858D1 (de) 1999-02-19 2010-03-18 Febit Holding Gmbh Verfahren zur Herstellung von Polymeren
ATE556149T1 (de) 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US20030186226A1 (en) 1999-03-08 2003-10-02 Brennan Thomas M. Methods and compositions for economically synthesizing and assembling long DNA sequences
US6824866B1 (en) 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US6284465B1 (en) 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US6469156B1 (en) 1999-04-20 2002-10-22 The United States Of America As Represented By The Department Of Health And Human Services Rapid and sensitive method for detecting histoplasma capsulatum
US6773676B2 (en) 1999-04-27 2004-08-10 Agilent Technologies, Inc. Devices for performing array hybridization assays and methods of using the same
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6518056B2 (en) 1999-04-27 2003-02-11 Agilent Technologies Inc. Apparatus, systems and method for assaying biological materials using an annular format
US6300137B1 (en) 1999-04-28 2001-10-09 Agilent Technologies Inc. Method for synthesizing a specific, surface-bound polymer uniformly over an element of a molecular array
US7276336B1 (en) 1999-07-22 2007-10-02 Agilent Technologies, Inc. Methods of fabricating an addressable array of biopolymer probes
US6323043B1 (en) 1999-04-30 2001-11-27 Agilent Technologies, Inc. Fabricating biopolymer arrays
US6242266B1 (en) 1999-04-30 2001-06-05 Agilent Technologies Inc. Preparation of biopolymer arrays
CA2368679C (en) 1999-05-01 2010-11-09 Qinetiq Limited Derivatized porous silicon
WO2000066259A1 (en) * 1999-05-05 2000-11-09 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
AU4703300A (en) 1999-05-06 2000-11-21 Mount Sinai School Of Medicine Of The City University Of New York, The Dna-based steganography
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
JP2003522119A (ja) 1999-05-24 2003-07-22 インビトロジェン コーポレイション 標識されたオリゴヌクレオチドの脱ブロック化方法
US6472147B1 (en) 1999-05-25 2002-10-29 The Scripps Research Institute Methods for display of heterodimeric proteins on filamentous phage using pVII and pIX, compositions, vectors and combinatorial libraries
US6132997A (en) 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6815218B1 (en) 1999-06-09 2004-11-09 Massachusetts Institute Of Technology Methods for manufacturing bioelectronic devices
US6709852B1 (en) 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
WO2000079009A2 (en) 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
DE19928410C2 (de) 1999-06-22 2002-11-28 Agilent Technologies Inc Gerätegehäuse mit einer Einrichtung zum Betrieb eines Labor-Mikrochips
US6399394B1 (en) 1999-06-30 2002-06-04 Agilent Technologies, Inc. Testing multiple fluid samples with multiple biopolymer arrays
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US6461816B1 (en) 1999-07-09 2002-10-08 Agilent Technologies, Inc. Methods for controlling cross-hybridization in analysis of nucleic acid sequences
US7504213B2 (en) 1999-07-09 2009-03-17 Agilent Technologies, Inc. Methods and apparatus for preparing arrays comprising features having degenerate biopolymers
US6346423B1 (en) 1999-07-16 2002-02-12 Agilent Technologies, Inc. Methods and compositions for producing biopolymeric arrays
US6306599B1 (en) 1999-07-16 2001-10-23 Agilent Technologies Inc. Biopolymer arrays and their fabrication
US6201112B1 (en) 1999-07-22 2001-03-13 Agilent Technologies Inc. Method for 3′ end-labeling ribonucleic acids
US6180351B1 (en) 1999-07-22 2001-01-30 Agilent Technologies Inc. Chemical array fabrication with identifier
CA2382157C (en) 1999-08-18 2012-04-03 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
US6262490B1 (en) 1999-11-05 2001-07-17 Advanced Semiconductor Engineering, Inc. Substrate strip for use in packaging semiconductor chips
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6319674B1 (en) 1999-09-16 2001-11-20 Agilent Technologies, Inc. Methods for attaching substances to surfaces
US6743585B2 (en) 1999-09-16 2004-06-01 Agilent Technologies, Inc. Methods for preparing conjugates
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7078167B2 (en) 1999-09-17 2006-07-18 Agilent Technologies, Inc. Arrays having background features and methods for using the same
US7122303B2 (en) 1999-09-17 2006-10-17 Agilent Technologies, Inc. Arrays comprising background features that provide for a measure of a non-specific binding and methods for using the same
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
DE19947495C2 (de) 1999-10-01 2003-05-28 Agilent Technologies Inc Mikrofluidischer Mikrochip
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6232072B1 (en) 1999-10-15 2001-05-15 Agilent Technologies, Inc. Biopolymer array inspection
US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
US6171797B1 (en) 1999-10-20 2001-01-09 Agilent Technologies Inc. Methods of making polymeric arrays
US6387636B1 (en) 1999-10-22 2002-05-14 Agilent Technologies, Inc. Method of shielding biosynthesis reactions from the ambient environment on an array
US7115423B1 (en) 1999-10-22 2006-10-03 Agilent Technologies, Inc. Fluidic structures within an array package
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6689319B1 (en) 1999-10-29 2004-02-10 Agilent Technologies, Ind. Apparatus for deposition and inspection of chemical and biological fluids
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US6329210B1 (en) 1999-10-29 2001-12-11 Agilent Technologies, Inc. Method and apparatus for high volume polymer synthesis
US20010055761A1 (en) 1999-10-29 2001-12-27 Agilent Technologies Small scale dna synthesis using polymeric solid support with functionalized regions
US6406849B1 (en) 1999-10-29 2002-06-18 Agilent Technologies, Inc. Interrogating multi-featured arrays
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US6440669B1 (en) 1999-11-10 2002-08-27 Agilent Technologies, Inc. Methods for applying small volumes of reagents
US7041445B2 (en) 1999-11-15 2006-05-09 Clontech Laboratories, Inc. Long oligonucleotide arrays
US6446642B1 (en) 1999-11-22 2002-09-10 Agilent Technologies, Inc. Method and apparatus to clean an inkjet reagent deposition device
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
CA2396320A1 (en) 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
EP1118661A1 (en) 2000-01-13 2001-07-25 Het Nederlands Kanker Instituut T cell receptor libraries
CA2398382A1 (en) 2000-01-25 2001-08-02 Affymetrix, Inc. Method, system and computer software for providing a genomic web portal
US6587579B1 (en) 2000-01-26 2003-07-01 Agilent Technologies Inc. Feature quality in array fabrication
US6406851B1 (en) 2000-01-28 2002-06-18 Agilent Technologies, Inc. Method for coating a substrate quickly and uniformly with a small volume of fluid
US7198939B2 (en) 2000-01-28 2007-04-03 Agilent Technologies, Inc. Apparatus for interrogating an addressable array
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US6403314B1 (en) 2000-02-04 2002-06-11 Agilent Technologies, Inc. Computational method and system for predicting fragmented hybridization and for identifying potential cross-hybridization
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6365355B1 (en) 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
US7776021B2 (en) 2000-04-28 2010-08-17 The Charles Stark Draper Laboratory Micromachined bilayer unit for filtration of small molecules
US6716634B1 (en) 2000-05-31 2004-04-06 Agilent Technologies, Inc. Increasing ionization efficiency in mass spectrometry
US7163660B2 (en) 2000-05-31 2007-01-16 Infineon Technologies Ag Arrangement for taking up liquid analytes
AU2001275091A1 (en) 2000-06-02 2001-12-17 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US7312043B2 (en) 2000-07-10 2007-12-25 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
JP2004504068A (ja) 2000-07-27 2004-02-12 ザ　オーストラリアン　ナショナル　ユニバーシティー コンビナトリアル・プローブ及びそのための用途
EP1176151B1 (en) 2000-07-28 2014-08-20 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
EP1180548B1 (en) 2000-07-31 2005-11-02 Agilent Technologies, Inc. (a Delaware corporation) Array based methods for synthesizing nucleic acid mixtures
US7205400B2 (en) 2000-07-31 2007-04-17 Agilent Technologies, Inc. Array fabrication
US6599693B1 (en) 2000-07-31 2003-07-29 Agilent Technologies Inc. Array fabrication
US6890760B1 (en) 2000-07-31 2005-05-10 Agilent Technologies, Inc. Array fabrication
US6613893B1 (en) 2000-07-31 2003-09-02 Agilent Technologies Inc. Array fabrication
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
JP2004527215A (ja) 2000-08-24 2004-09-09 マキシジェン， インコーポレイテッド 構築物、および代謝経路操作におけるそれらの使用
US20030036066A1 (en) 2000-09-08 2003-02-20 University Technologies International Inc. Linker phosphoramidites for oligonucleotide synthesis
US6966945B1 (en) 2000-09-20 2005-11-22 Goodrich Corporation Inorganic matrix compositions, composites and process of making the same
US6897023B2 (en) 2000-09-27 2005-05-24 The Molecular Sciences Institute, Inc. Method for determining relative abundance of nucleic acid sequences
NO20004869D0 (no) 2000-09-28 2000-09-28 Torbjoern Rognes Metode for hurtig optimal lokal sekvensjustering ved bruk av parallell prosessering
US7097809B2 (en) 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
EP1330306A2 (en) 2000-10-10 2003-07-30 BioTrove, Inc. Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
DE10051396A1 (de) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh Verfahren und Vorrichtung zur integrierten Synthese und Analytbestimmung an einem Träger
US6693187B1 (en) 2000-10-17 2004-02-17 Lievre Cornu Llc Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge
PT1327230E (pt) 2000-10-18 2005-10-31 Ultra Proizv Elektronskih Napr Sistema para intercambio de dados de pagamento e dispositivo de terminal de pagamento para uso no mesmo
DE60125312T2 (de) 2000-10-26 2007-06-06 Agilent Technologies, Inc. (n.d. Ges. d. Staates Delaware), Santa Clara Mikroarray
US6905816B2 (en) 2000-11-27 2005-06-14 Intelligent Medical Devices, Inc. Clinically intelligent diagnostic devices and methods
US20020155439A1 (en) 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
US20040253242A1 (en) 2000-12-05 2004-12-16 Bowdish Katherine S. Rationally designed antibodies
DE10060433B4 (de) 2000-12-05 2006-05-11 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Verfahren zur Herstellung eines Fluidbauelements, Fluidbauelement und Analysevorrichtung
EP2332954A3 (en) 2000-12-05 2011-12-21 Avecia Biotechnology, Inc. Process for the preparation of phosphorothionate oligonucleotides
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
AUPR259301A0 (en) 2001-01-18 2001-02-15 Polymerat Pty Ltd Polymers having co-continuous architecture
US20030138782A1 (en) 2001-01-19 2003-07-24 Evans Glen A. Computer-directed assembly of a polynucleotide encoding a target polypeptide
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US6879915B2 (en) 2001-01-31 2005-04-12 Agilent Technologies, Inc. Chemical array fabrication and use
US7027930B2 (en) 2001-01-31 2006-04-11 Agilent Technologies, Inc. Reading chemical arrays
US7166258B2 (en) 2001-01-31 2007-01-23 Agilent Technologies, Inc. Automation-optimized microarray package
US20020164824A1 (en) 2001-02-16 2002-11-07 Jianming Xiao Method and apparatus based on bundled capillaries for high throughput screening
US6660338B1 (en) 2001-03-08 2003-12-09 Agilent Technologies, Inc. Functionalization of substrate surfaces with silane mixtures
DE60207995T2 (de) 2001-03-08 2006-08-03 Applera Corp., Foster City Verfahren zum entschützen von oligonukleotiden
US7211654B2 (en) * 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US6610978B2 (en) 2001-03-27 2003-08-26 Agilent Technologies, Inc. Integrated sample preparation, separation and introduction microdevice for inductively coupled plasma mass spectrometry
US7179659B2 (en) 2001-04-02 2007-02-20 Agilent Technologies, Inc. Sensor surfaces for detecting analytes and methods of use
US20030022240A1 (en) 2001-04-17 2003-01-30 Peizhi Luo Generation and affinity maturation of antibody library in silico
US6943036B2 (en) 2001-04-30 2005-09-13 Agilent Technologies, Inc. Error detection in chemical array fabrication
CA2446417A1 (en) 2001-05-03 2002-11-14 Sigma Genosys, L.P. Methods for assembling protein microarrays
WO2002095073A1 (en) 2001-05-18 2002-11-28 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
WO2002094846A2 (en) 2001-05-22 2002-11-28 Parallel Synthesis Technologies, Inc. Method for in situ, on-chip chemical synthesis
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US6649348B2 (en) 2001-06-29 2003-11-18 Agilent Technologies Inc. Methods for manufacturing arrays
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US6989267B2 (en) 2001-07-02 2006-01-24 Agilent Technologies, Inc. Methods of making microarrays with substrate surfaces having covalently bound polyelectrolyte films
US6753145B2 (en) 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
US7205399B1 (en) 2001-07-06 2007-04-17 Sirna Therapeutics, Inc. Methods and reagents for oligonucleotide synthesis
US7314599B2 (en) 2001-07-17 2008-01-01 Agilent Technologies, Inc. Paek embossing and adhesion for microfluidic devices
US6702256B2 (en) 2001-07-17 2004-03-09 Agilent Technologies, Inc. Flow-switching microdevice
US7128876B2 (en) 2001-07-17 2006-10-31 Agilent Technologies, Inc. Microdevice and method for component separation in a fluid
US20030108903A1 (en) 2001-07-19 2003-06-12 Liman Wang Multiple word DNA computing on surfaces
ATE432981T1 (de) 2001-07-26 2009-06-15 Stratagene California Multi-stellen-mutagenese
WO2003014325A2 (en) 2001-08-10 2003-02-20 Xencor Protein design automation for protein libraries
US6682702B2 (en) 2001-08-24 2004-01-27 Agilent Technologies, Inc. Apparatus and method for simultaneously conducting multiple chemical reactions
US7371580B2 (en) 2001-08-24 2008-05-13 Agilent Technologies, Inc. Use of unstructured nucleic acids in assaying nucleic acid molecules
JP2003101204A (ja) 2001-09-25 2003-04-04 Nec Kansai Ltd 配線基板及び配線基板の製造方法並びに電子部品
US20050124022A1 (en) 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US7524950B2 (en) 2001-10-31 2009-04-28 Agilent Technologies, Inc. Uses of cationic salts for polynucleotide synthesis
US6852850B2 (en) 2001-10-31 2005-02-08 Agilent Technologies, Inc. Use of ionic liquids for fabrication of polynucleotide arrays
US6858720B2 (en) 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US20030087298A1 (en) 2001-11-02 2003-05-08 Roland Green Detection of hybridization on oligonucleotide microarray through covalently labeling microarray probe
AU2002360361A1 (en) 2001-11-09 2003-06-10 Biomicroarrays, Inc. High surface area substrates for microarrays and methods to make same
US7482118B2 (en) 2001-11-15 2009-01-27 Third Wave Technologies, Inc. Endonuclease-substrate complexes
DK1314783T3 (da) 2001-11-22 2009-03-16 Sloning Biotechnology Gmbh Nukleinsyrelinkere og deres anvendelse i gensyntese
US20030099952A1 (en) 2001-11-26 2003-05-29 Roland Green Microarrays with visible pattern detection
WO2003048395A1 (en) 2001-12-03 2003-06-12 Zymogenetics, Inc. Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
US6838888B2 (en) 2001-12-13 2005-01-04 Agilent Technologies, Inc. Flow cell humidity sensor system
AU2002357249A1 (en) 2001-12-13 2003-07-09 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US6790620B2 (en) 2001-12-24 2004-09-14 Agilent Technologies, Inc. Small volume chambers
US7282183B2 (en) 2001-12-24 2007-10-16 Agilent Technologies, Inc. Atmospheric control in reaction chambers
US6846454B2 (en) 2001-12-24 2005-01-25 Agilent Technologies, Inc. Fluid exit in reaction chambers
WO2003057924A1 (en) * 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
US7025324B1 (en) 2002-01-04 2006-04-11 Massachusetts Institute Of Technology Gating apparatus and method of manufacture
US20040009498A1 (en) 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
US6673552B2 (en) 2002-01-14 2004-01-06 Diversa Corporation Methods for purifying annealed double-stranded oligonucleotides lacking base pair mismatches or nucleotide gaps
EP1472373A4 (en) 2002-01-14 2005-07-27 Diversa Corp PROCESSES FOR THE PRODUCTION AND PURIFICATION OF DOUBLE STRANDED POLYNUCLEOTIDES
US7141368B2 (en) 2002-01-30 2006-11-28 Agilent Technologies, Inc. Multi-directional deposition in array fabrication
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
US7037659B2 (en) 2002-01-31 2006-05-02 Nimblegen Systems Inc. Apparatus for constructing DNA probes having a prismatic and kaleidoscopic light homogenizer
US20040126757A1 (en) 2002-01-31 2004-07-01 Francesco Cerrina Method and apparatus for synthesis of arrays of DNA probes
US7083975B2 (en) 2002-02-01 2006-08-01 Roland Green Microarray synthesis instrument and method
US20030148291A1 (en) 2002-02-05 2003-08-07 Karla Robotti Method of immobilizing biologically active molecules for assay purposes in a microfluidic format
US6728129B2 (en) 2002-02-19 2004-04-27 The Regents Of The University Of California Multistate triple-decker dyads in three distinct architectures for information storage applications
US6958119B2 (en) 2002-02-26 2005-10-25 Agilent Technologies, Inc. Mobile phase gradient generation microfluidic device
US6770892B2 (en) 2002-02-28 2004-08-03 Agilent Technologies, Inc. Method and system for automated focus-distance determination for molecular array scanners
US6929951B2 (en) 2002-02-28 2005-08-16 Agilent Technologies, Inc. Method and system for molecular array scanner calibration
US6914229B2 (en) 2002-02-28 2005-07-05 Agilent Technologies, Inc. Signal offset for prevention of data clipping in a molecular array scanner
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US6919181B2 (en) 2002-03-25 2005-07-19 Agilent Technologies, Inc. Methods for generating ligand arrays
US20030228602A1 (en) 2002-04-01 2003-12-11 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
EP1350853A1 (en) 2002-04-05 2003-10-08 ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V. Detection of polymorphisms
US6773888B2 (en) 2002-04-08 2004-08-10 Affymetrix, Inc. Photoactivatable silane compounds and methods for their synthesis and use
DK1501947T3 (da) 2002-04-22 2008-11-17 Genencor Int Fremgangsmåde til at skabe et bibliotek af bakterielle kloner med varierende niveauer af genekspression
GB0209539D0 (en) 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
US6946285B2 (en) 2002-04-29 2005-09-20 Agilent Technologies, Inc. Arrays with elongated features
US7125523B2 (en) 2002-04-29 2006-10-24 Agilent Technologies, Inc. Holders for arrays
US7094537B2 (en) 2002-04-30 2006-08-22 Agilent Technologies, Inc. Micro arrays with structured and unstructured probes
US6621076B1 (en) 2002-04-30 2003-09-16 Agilent Technologies, Inc. Flexible assembly for transporting sample fluids into a mass spectrometer
AU2003233243A1 (en) 2002-05-06 2003-11-17 Noxxon Pharma Ag Method for amplifying nucleic acids
US20030211478A1 (en) 2002-05-08 2003-11-13 Gentel Corporation Transcription factor profiling on a solid surface
US7221785B2 (en) 2002-05-21 2007-05-22 Agilent Technologies, Inc. Method and system for measuring a molecular array background signal from a continuous background region of specified size
WO2003100019A2 (en) 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
CA2487093A1 (en) 2002-05-24 2003-12-04 Nimblegen Systems, Inc. Microarrays and method for running hybridization reaction for multiple samples on a single microarray
US7537936B2 (en) 2002-05-31 2009-05-26 Agilent Technologies, Inc. Method of testing multiple fluid samples with multiple biopolymer arrays
US6789965B2 (en) 2002-05-31 2004-09-14 Agilent Technologies, Inc. Dot printer with off-axis loading
US7078505B2 (en) 2002-06-06 2006-07-18 Agilent Technologies, Inc. Manufacture of arrays with varying deposition parameters
US7351379B2 (en) 2002-06-14 2008-04-01 Agilent Technologies, Inc. Fluid containment structure
US6939673B2 (en) 2002-06-14 2005-09-06 Agilent Technologies, Inc. Manufacture of arrays with reduced error impact
US7919308B2 (en) 2002-06-14 2011-04-05 Agilent Technologies, Inc. Form in place gaskets for assays
US7371348B2 (en) 2002-06-14 2008-05-13 Agilent Technologies Multiple array format
US20070275411A1 (en) * 2006-05-25 2007-11-29 Mcgall Glenn H Silane mixtures
US7220573B2 (en) 2002-06-21 2007-05-22 Agilent Technologies, Inc. Array assay devices and methods of using the same
US6713262B2 (en) 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
US7894998B2 (en) 2002-06-26 2011-02-22 Agilent Technologies, Inc. Method for identifying suitable nucleic acid probe sequences for use in nucleic acid arrays
US7202358B2 (en) 2002-07-25 2007-04-10 Agilent Technologies, Inc. Methods for producing ligand arrays
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US6835938B2 (en) 2002-07-31 2004-12-28 Agilent Technologies, Inc. Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners
US7101508B2 (en) 2002-07-31 2006-09-05 Agilent Technologies, Inc. Chemical array fabrication errors
US7153689B2 (en) 2002-08-01 2006-12-26 Agilent Technologies, Inc. Apparatus and methods for cleaning and priming droplet dispensing devices
US8946387B2 (en) 2002-08-14 2015-02-03 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US7205128B2 (en) 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
JP2006517090A (ja) 2002-09-26 2006-07-20 コーサン バイオサイエンシーズ， インコーポレイテッド 合成遺伝子
AU2003270898A1 (en) 2002-09-27 2004-04-19 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
US7482170B2 (en) 2002-09-30 2009-01-27 Roche Nimblegen, Inc. Parallel loading of arrays
CA2500783C (en) 2002-10-01 2012-07-17 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
US7129075B2 (en) 2002-10-18 2006-10-31 Transgenomic, Inc. Isolated CEL II endonuclease
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
US20070059692A1 (en) 2002-10-28 2007-03-15 Xiaolian Gao Array oligomer synthesis and use
US7390457B2 (en) 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
US7364896B2 (en) 2002-10-31 2008-04-29 Agilent Technologies, Inc. Test strips including flexible array substrates and method of hybridization
WO2004040295A1 (en) 2002-10-31 2004-05-13 Nanostream, Inc. Parallel detection chromatography systems
US7402279B2 (en) 2002-10-31 2008-07-22 Agilent Technologies, Inc. Device with integrated microfluidic and electronic components
US7422911B2 (en) 2002-10-31 2008-09-09 Agilent Technologies, Inc. Composite flexible array substrate having flexible support
US7629120B2 (en) 2002-10-31 2009-12-08 Rice University Method for assembling PCR fragments of DNA
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
US7029854B2 (en) 2002-11-22 2006-04-18 Agilent Technologies, Inc. Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays
US7062385B2 (en) 2002-11-25 2006-06-13 Tufts University Intelligent electro-optical nucleic acid-based sensor array and method for detecting volatile compounds in ambient air
US7285269B2 (en) 2002-12-02 2007-10-23 Amgen Fremont, Inc. Antibodies directed to tumor necrosis factor
US20040110133A1 (en) 2002-12-06 2004-06-10 Affymetrix, Inc. Functionated photoacid generator for biological microarray synthesis
US7879580B2 (en) 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US6987263B2 (en) 2002-12-13 2006-01-17 Nanostream, Inc. High throughput systems and methods for parallel sample analysis
US20060076482A1 (en) 2002-12-13 2006-04-13 Hobbs Steven E High throughput systems and methods for parallel sample analysis
US7247337B1 (en) 2002-12-16 2007-07-24 Agilent Technologies, Inc. Method and apparatus for microarray fabrication
US20040191810A1 (en) 2002-12-17 2004-09-30 Affymetrix, Inc. Immersed microarrays in conical wells
GB0229443D0 (en) 2002-12-18 2003-01-22 Avecia Ltd Process
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US8232055B2 (en) * 2002-12-23 2012-07-31 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same
CN1753912B (zh) 2002-12-23 2011-11-02 惠氏公司 抗pd-1抗体及其用途
WO2004058392A2 (de) 2002-12-23 2004-07-15 Febit Ag Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
DE10260805A1 (de) 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
US7372982B2 (en) 2003-01-14 2008-05-13 Agilent Technologies, Inc. User interface for molecular array feature analysis
US6809277B2 (en) 2003-01-22 2004-10-26 Agilent Technologies, Inc. Method for registering a deposited material with channel plate channels, and switch produced using same
AU2004209001B2 (en) 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US8073626B2 (en) 2003-01-31 2011-12-06 Agilent Technologies, Inc. Biopolymer array reading
US7202264B2 (en) 2003-01-31 2007-04-10 Isis Pharmaceuticals, Inc. Supports for oligomer synthesis
US6950756B2 (en) 2003-02-05 2005-09-27 Agilent Technologies, Inc. Rearrangement of microarray scan images to form virtual arrays
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7413709B2 (en) 2003-02-12 2008-08-19 Agilent Technologies, Inc. PAEK-based microfluidic device with integrated electrospray emitter
US7244513B2 (en) 2003-02-21 2007-07-17 Nano-Proprietary, Inc. Stain-etched silicon powder
US7070932B2 (en) 2003-02-25 2006-07-04 Agilent Technologies, Inc. Methods and devices for detecting printhead misalignment of an in situ polymeric array synthesis device
US7252938B2 (en) 2003-02-25 2007-08-07 Agilent Technologies, Inc. Methods and devices for producing a polymer at a location of a substrate
JP2004268394A (ja) 2003-03-07 2004-09-30 Canon Inc インクジェット記録装置及びその制御方法
WO2004080887A1 (en) 2003-03-07 2004-09-23 Massachusetts Institute Of Technology Three dimensional mecrofabrication
US20050053968A1 (en) 2003-03-31 2005-03-10 Council Of Scientific And Industrial Research Method for storing information in DNA
WO2004090170A1 (en) 2003-04-02 2004-10-21 Blue Heron Biotechnology, Inc. Error reduction in automated gene synthesis
US7534561B2 (en) 2003-04-02 2009-05-19 Agilent Technologies, Inc. Nucleic acid array in situ fabrication methods and arrays produced using the same
US20040219663A1 (en) 2003-04-30 2004-11-04 Page Robert D. Biopolymer array fabrication using different drop deposition heads
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US7206439B2 (en) 2003-04-30 2007-04-17 Agilent Technologies, Inc. Feature locations in array reading
US6916113B2 (en) 2003-05-16 2005-07-12 Agilent Technologies, Inc. Devices and methods for fluid mixing
EP1636017A2 (en) 2003-05-20 2006-03-22 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
AU2004251256B2 (en) 2003-05-30 2009-05-28 The Board Of Trustees Of The University Of Illinois Gene expression profiles that identify genetically elite ungulate mammals
EP1660512A4 (en) 2003-06-02 2009-12-23 Isis Pharmaceuticals Inc SYNTHESIS OF OLIGONUCLEOTIDES USING ALTERNATIVE SOLVENTS
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
US6938476B2 (en) 2003-06-25 2005-09-06 Agilent Technologies, Inc. Apparatus and methods for sensing fluid levels
US7534563B2 (en) 2003-06-30 2009-05-19 Agilent Technologies, Inc. Methods for producing ligand arrays
US20050016851A1 (en) 2003-07-24 2005-01-27 Jensen Klavs F. Microchemical method and apparatus for synthesis and coating of colloidal nanoparticles
US6843281B1 (en) 2003-07-30 2005-01-18 Agilent Techinologies, Inc. Methods and apparatus for introducing liquids into microfluidic chambers
US7353116B2 (en) 2003-07-31 2008-04-01 Agilent Technologies, Inc. Chemical array with test dependent signal reading or processing
US7939310B2 (en) 2003-08-06 2011-05-10 University Of Massachusetts Systems and methods for analyzing nucleic acid sequences
US7028536B2 (en) 2004-06-29 2006-04-18 Nanostream, Inc. Sealing interface for microfluidic device
US7348144B2 (en) 2003-08-13 2008-03-25 Agilent Technologies, Inc. Methods and system for multi-drug treatment discovery
US7229497B2 (en) 2003-08-26 2007-06-12 Massachusetts Institute Of Technology Method of preparing nanocrystals
US7585970B2 (en) 2003-08-30 2009-09-08 Agilent Technologies, Inc. Method of polynucleotide synthesis using modified support
US7385050B2 (en) 2003-08-30 2008-06-10 Agilent Technologies, Inc. Cleavable linker for polynucleotide synthesis
US7417139B2 (en) 2003-08-30 2008-08-26 Agilent Technologies, Inc. Method for polynucleotide synthesis
US7427679B2 (en) 2003-08-30 2008-09-23 Agilent Technologies, Inc. Precursors for two-step polynucleotide synthesis
US7193077B2 (en) 2003-08-30 2007-03-20 Agilent Technologies, Inc. Exocyclic amine triaryl methyl protecting groups in two-step polynucleotide synthesis
US20050049796A1 (en) 2003-09-03 2005-03-03 Webb Peter G. Methods for encoding non-biological information on microarrays
US7362901B2 (en) 2003-09-05 2008-04-22 Gannon Technology Holdings Llc Systems and methods for biometric identification using handwriting recognition
EP2325339A3 (en) 2003-09-09 2011-11-02 Integrigen, Inc. Methods and compositions for generation of germline human antibody genes
WO2005030929A2 (en) 2003-09-23 2005-04-07 Atom Sciences, Inc. Polymeric nucleic acid hybridization probes
US7488607B2 (en) 2003-09-30 2009-02-10 Agilent Technologies, Inc. Electronically readable microarray with electronic addressing function
US7147362B2 (en) 2003-10-15 2006-12-12 Agilent Technologies, Inc. Method of mixing by intermittent centrifugal force
US7075161B2 (en) 2003-10-23 2006-07-11 Agilent Technologies, Inc. Apparatus and method for making a low capacitance artificial nanopore
WO2005043154A2 (en) 2003-10-27 2005-05-12 Massachusetts Institute Of Technology High density reaction chambers and methods of use
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7276338B2 (en) 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
DE10353887A1 (de) 2003-11-18 2005-06-16 Febit Ag Hochparalleler DNA-Synthesizer auf Matrizenbasis
US7851192B2 (en) 2004-11-22 2010-12-14 New England Biolabs, Inc. Modified DNA cleavage enzymes and methods for use
US7282705B2 (en) 2003-12-19 2007-10-16 Agilent Technologies, Inc. Microdevice having an annular lining for producing an electrospray emitter
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
US7084180B2 (en) 2004-01-28 2006-08-01 Velocys, Inc. Fischer-tropsch synthesis using microchannel technology and novel catalyst and microchannel reactor
US7927797B2 (en) 2004-01-28 2011-04-19 454 Life Sciences Corporation Nucleic acid amplification with continuous flow emulsion
US7125488B2 (en) 2004-02-12 2006-10-24 Varian, Inc. Polar-modified bonded phase materials for chromatographic separations
DE602005018166D1 (de) 2004-02-12 2010-01-21 Population Genetics Technologi Genetische analyse mittels sequenzspezifischem sortieren
WO2005089110A2 (en) 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7875463B2 (en) 2004-03-26 2011-01-25 Agilent Technologies, Inc. Generalized pulse jet ejection head control model
WO2005093092A2 (en) 2004-03-26 2005-10-06 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with g-protein coupled receptor 44 (gpr44)
US20050214779A1 (en) 2004-03-29 2005-09-29 Peck Bill J Methods for in situ generation of nucleic acid arrays
US20050214778A1 (en) 2004-03-29 2005-09-29 Peck Bill J Methods for in situ generation of nucleic acid arrays
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
CA2566866A1 (en) 2004-05-11 2006-03-09 Wyeth Novel polynucleotides related to oligonucleotide arrays to monitor gene expression
DK1773978T3 (da) 2004-05-19 2014-05-26 Univ Pittsburgh Perfunderede, tredimensionelle celle/vævssygdomsmodeller
US7302348B2 (en) 2004-06-02 2007-11-27 Agilent Technologies, Inc. Method and system for quantifying and removing spatial-intensity trends in microarray data
US20060002471A1 (en) 2004-06-30 2006-01-05 Lippincott Louis A Motion estimation unit
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
ATE528396T1 (de) 2004-07-06 2011-10-15 Bioren Inc Look-through-mutagenese zur entwicklung veränderter polypeptide mit verbesserten eigenschaften
WO2006014533A2 (en) 2004-07-07 2006-02-09 Home Guardian Llc Instrumented mobility assistance device
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060019084A1 (en) 2004-07-23 2006-01-26 Pearson Laurence T Monolithic composition and method
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
KR101222628B1 (ko) 2004-08-03 2013-01-16 게네아르트 아게 ＣｐＧ 함량을 변화시키는 것에 의해 유전자 발현을조절하는 방법
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US7034290B2 (en) 2004-09-24 2006-04-25 Agilent Technologies, Inc. Target support with pattern recognition sites
US7943046B2 (en) 2004-10-01 2011-05-17 Agilent Technologies, Inc Methods and systems for on-column protein delipidation
CA2584984A1 (en) 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
US7141807B2 (en) 2004-10-22 2006-11-28 Agilent Technologies, Inc. Nanowire capillaries for mass spectrometry
US20060110744A1 (en) 2004-11-23 2006-05-25 Sampas Nicolas M Probe design methods and microarrays for comparative genomic hybridization and location analysis
US8380441B2 (en) 2004-11-30 2013-02-19 Agilent Technologies, Inc. Systems for producing chemical array layouts
US11268149B2 (en) 2004-12-08 2022-03-08 Cedars-Sinai Medical Center Diagnosis and treatment of inflammatory bowel disease
US7977119B2 (en) 2004-12-08 2011-07-12 Agilent Technologies, Inc. Chemical arrays and methods of using the same
US7439272B2 (en) 2004-12-20 2008-10-21 Varian, Inc. Ultraporous sol gel monoliths
WO2006068625A1 (en) 2004-12-22 2006-06-29 National University Of Singapore Novel snake toxin
US20070099196A1 (en) 2004-12-29 2007-05-03 Sakari Kauppinen Novel oligonucleotide compositions and probe sequences useful for detection and analysis of micrornas and their target mRNAs
WO2006076679A1 (en) 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
US20060171855A1 (en) 2005-02-03 2006-08-03 Hongfeng Yin Devices,systems and methods for multi-dimensional separation
WO2006086391A2 (en) 2005-02-07 2006-08-17 Massachusetts Institute Of Technology Electrochemically-degradable layer-by-layer thin films
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP4641199B2 (ja) 2005-02-28 2011-03-02 国立感染症研究所長 Ｒｎａ干渉ポリヌクレオチド混合物の設計装置、ｒｎａ干渉ポリヌクレオチド混合物の作製方法、及びｒｎａ干渉ポリヌクレオチド混合物の設計プログラム
US20060203236A1 (en) 2005-03-08 2006-09-14 Zhenghua Ji Sample cell
EP1623763A1 (en) 2005-03-11 2006-02-08 Agilent Technologies, Inc. Chip with cleaning cavity
US7618777B2 (en) 2005-03-16 2009-11-17 Agilent Technologies, Inc. Composition and method for array hybridization
US20060219637A1 (en) 2005-03-29 2006-10-05 Killeen Kevin P Devices, systems and methods for liquid chromatography
WO2006116476A1 (en) 2005-04-27 2006-11-02 Sigma-Aldrich Co. Activators for oligonucleotide and phosphoramidite synthesis
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
WO2006119066A2 (en) 2005-04-29 2006-11-09 The J. Craig Venter Institute Amplification and cloning of single dna molecules using rolling circle amplification
RU2435809C2 (ru) 2005-05-12 2011-12-10 Хемпель А/С Способ образования устойчивого к трещинообразованию лакокрасочного эпоксидного покрытия и лакокрасочная композиция, подходящая для такого способа
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7919239B2 (en) 2005-07-01 2011-04-05 Agilent Technologies, Inc. Increasing hybridization efficiencies
US7718365B2 (en) 2005-07-09 2010-05-18 Agilent Technologies, Inc. Microarray analysis of RNA
US8076064B2 (en) 2005-07-09 2011-12-13 Agilent Technologies, Inc. Method of treatment of RNA sample
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
DE102005037351B3 (de) 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
US9404882B2 (en) 2005-08-11 2016-08-02 New Mexico Tech Research Foundation Method of producing a multi-microchannel, flow-through element and device using same
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
CA2618665C (en) 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
US7749701B2 (en) 2005-08-11 2010-07-06 Agilent Technologies, Inc. Controlling use of oligonucleotide sequences released from arrays
US7805252B2 (en) 2005-08-16 2010-09-28 Dna Twopointo, Inc. Systems and methods for designing and ordering polynucleotides
WO2007025059A1 (en) 2005-08-26 2007-03-01 Surmodics, Inc. Silane coating compositions, coating systems, and methods
US20070196834A1 (en) 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
JP5055282B2 (ja) * 2005-09-14 2012-10-24 イルミナ インコーポレイテッド 連続的なポリマー合成器
US20100233429A1 (en) 2005-09-16 2010-09-16 Yamatake Corporation Substrate for Biochip, Biochip, Method for Manufacturing Substrate for Biochip and Method for Manufacturing Biochip
US20080308884A1 (en) 2005-10-13 2008-12-18 Silex Microsystems Ab Fabrication of Inlet and Outlet Connections for Microfluidic Chips
US7368550B2 (en) 2005-10-31 2008-05-06 Agilent Technologies, Inc. Phosphorus protecting groups
US8202985B2 (en) 2005-10-31 2012-06-19 Agilent Technologies, Inc. Monomer compositions for the synthesis of polynucleotides, methods of synthesis, and methods of deprotection
US8552174B2 (en) 2005-10-31 2013-10-08 Agilent Technologies, Inc. Solutions, methods, and processes for deprotection of polynucleotides
US7759471B2 (en) 2005-10-31 2010-07-20 Agilent Technologies, Inc. Monomer compositions for the synthesis of RNA, methods of synthesis, and methods of deprotection
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7291471B2 (en) 2005-11-21 2007-11-06 Agilent Technologies, Inc. Cleavable oligonucleotide arrays
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US8137936B2 (en) 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
EP1966394B1 (en) 2005-12-22 2012-07-25 Keygene N.V. Improved strategies for transcript profiling using high throughput sequencing technologies
JP5106416B2 (ja) 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
US20070207487A1 (en) 2006-01-25 2007-09-06 Emig Christopher J Photoelectrochemical synthesis of high density combinatorial polymer arrays
WO2008057127A2 (en) 2006-02-06 2008-05-15 Massachusetts Institute Of Technology Self-assembly of macromolecules on multilayered polymer surfaces
EP1987275A1 (en) 2006-02-13 2008-11-05 Koninklijke Philips Electronics N.V. Microfluidic device for molecular diagnostic applications
US8809876B2 (en) 2006-02-14 2014-08-19 Massachusetts Institute Of Technology Absorbing film
US7807356B2 (en) 2006-03-09 2010-10-05 Agilent Technologies, Inc. Labeled nucleotide composition
TW200806317A (en) 2006-03-20 2008-02-01 Wyeth Corp Methods for reducing protein aggregation
US7572908B2 (en) 2006-03-23 2009-08-11 Agilent Technologies, Inc. Cleavable linkers for polynucleotides
US7855281B2 (en) 2006-03-23 2010-12-21 Agilent Technologies, Inc. Cleavable thiocarbonate linkers for polynucleotide synthesis
US20070231800A1 (en) 2006-03-28 2007-10-04 Agilent Technologies, Inc. Determination of methylated DNA
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US20070238104A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Competitive oligonucleotides
US20070238108A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US20070238106A1 (en) 2006-04-07 2007-10-11 Agilent Technologies, Inc. Systems and methods of determining alleles and/or copy numbers
CN101578297A (zh) 2006-04-07 2009-11-11 美国政府健康及人类服务部 治疗肿瘤疾病的抗体组合物和方法
US8058055B2 (en) 2006-04-07 2011-11-15 Agilent Technologies, Inc. High resolution chromosomal mapping
JP5654749B2 (ja) 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US20070259345A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Target determination using compound probes
US20070259346A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Analysis of arrays
US20070259344A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Compound probes and methods of increasing the effective probe densities of arrays
US20070259347A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Methods of increasing the effective probe densities of arrays
US20070281309A1 (en) 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
WO2007136834A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008054543A2 (en) * 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly
US7624114B2 (en) 2006-06-05 2009-11-24 Microsoft Corporation Automatically generating web forms from database schema
ES2551577T3 (es) 2006-06-19 2015-11-20 Yeda Research And Development Company Limited Alargamiento Iterado Programable: Un método para elaborar genes sintéticos y ADN combinatorio y librerías de proteína
AT503861B1 (de) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw Verfahren zur manipulation von t-zell-rezeptoren
AT503902B1 (de) 2006-07-05 2008-06-15 F Star Biotech Forsch & Entw Verfahren zur manipulation von immunglobulinen
US20080193772A1 (en) 2006-07-07 2008-08-14 Bio-Rad Laboratories, Inc Mass spectrometry probes having hydrophobic coatiings
US7572585B2 (en) 2006-07-31 2009-08-11 Agilent Technologies, Inc. Enzymatic labeling of RNA
US7524942B2 (en) 2006-07-31 2009-04-28 Agilent Technologies, Inc. Labeled nucleotide composition
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
EP4082551A1 (en) 2006-08-08 2022-11-02 Rheinische Friedrich-Wilhelms-Universität Bonn Structure and use of 5' phosphate oligonucleotides
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
WO2008023179A2 (en) 2006-08-24 2008-02-28 Solexa Limited Method for retaining even coverage of short insert libraries
US8415138B2 (en) 2006-08-31 2013-04-09 Agilent Technologies, Inc. Apparatuses and methods for oligonucleotide preparation
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US8097711B2 (en) 2006-09-02 2012-01-17 Agilent Technologies, Inc. Thioether substituted aryl carbonate protecting groups
US20080311628A1 (en) 2006-10-03 2008-12-18 Ghc Technologies, Inc. Methods and compositions for rapid amplification and capture of nucleic acid sequences
EP2078077A2 (en) 2006-10-04 2009-07-15 Codon Devices, Inc Nucleic acid libraries and their design and assembly
US20080085511A1 (en) * 2006-10-05 2008-04-10 Peck Bill J Preparation of biopolymer arrays
US20080085514A1 (en) 2006-10-10 2008-04-10 Peck Bill J Methods and devices for array synthesis
JP2008097189A (ja) 2006-10-10 2008-04-24 National Institute Of Advanced Industrial & Technology 塩基配列断片の転写物特異性又は遺伝子特異性を判定する方法
US7867782B2 (en) 2006-10-19 2011-01-11 Agilent Technologies, Inc. Nanoscale moiety placement methods
US7999087B2 (en) 2006-11-15 2011-08-16 Agilent Technologies, Inc. 2′-silyl containing thiocarbonate protecting groups for RNA synthesis
WO2008063134A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Method of producing a pattern of discriminative wettability
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US8242258B2 (en) 2006-12-03 2012-08-14 Agilent Technologies, Inc. Protecting groups for RNA synthesis
US20080267949A1 (en) 2006-12-05 2008-10-30 Ablynx N.V. Peptides capable of binding to serum proteins
US7989396B2 (en) 2006-12-05 2011-08-02 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule immobilization on biosensors
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US8314220B2 (en) 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA
US20080182296A1 (en) 2007-01-31 2008-07-31 Chanda Pranab K Pcr-directed gene synthesis from large number of overlapping oligodeoxyribonucleotides
KR100827449B1 (ko) 2007-02-07 2008-05-07 삼성전자주식회사 광분해성 화합물과 상기 화합물이 커플링된 올리고머프로브 어레이용 기판, 올리고머 프로브 어레이 및 이의제조 방법
US20100323404A1 (en) 2007-02-09 2010-12-23 Richard Lathrop Method for recombining dna sequences and compositions related thereto
BRPI0807952A2 (pt) 2007-02-20 2014-06-10 Anaptysbio Inc Sistemas de hipermutação somática
JP2008218579A (ja) 2007-03-01 2008-09-18 Denki Kagaku Kogyo Kk 金属ベース回路基板
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US7651762B2 (en) 2007-03-13 2010-01-26 Varian, Inc. Methods and devices using a shrinkable support for porous monolithic materials
US8500982B2 (en) 2007-04-04 2013-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
CN101784556B (zh) 2007-05-10 2014-07-09 安捷伦科技有限公司 用于rna合成的硫代碳保护基团
WO2008148392A1 (en) 2007-06-04 2008-12-11 In Situ Rcp A/S Enzyme activity assay using rolling circle amplification
US20080318334A1 (en) 2007-06-20 2008-12-25 Robotti Karla M Microfluidic devices comprising fluid flow paths having a monolithic chromatographic material
US20090023190A1 (en) 2007-06-20 2009-01-22 Kai Qin Lao Sequence amplification with loopable primers
US8194244B2 (en) 2007-06-29 2012-06-05 Intel Corporation Solution sample plate with wells designed for improved Raman scattering signal detection efficiency
US7659069B2 (en) 2007-08-31 2010-02-09 Agilent Technologies, Inc. Binary signaling assay using a split-polymerase
US8685642B2 (en) 2007-07-30 2014-04-01 Agilent Technologies, Inc. Allele-specific copy number measurement using single nucleotide polymorphism and DNA arrays
US7979215B2 (en) 2007-07-30 2011-07-12 Agilent Technologies, Inc. Methods and systems for evaluating CGH candidate probe nucleic acid sequences
US20090036664A1 (en) 2007-07-31 2009-02-05 Brian Jon Peter Complex oligonucleotide primer mix
WO2009020435A1 (en) 2007-08-07 2009-02-12 Agency For Science, Technology And Research Integrated microfluidic device for gene synthesis
US9440231B2 (en) 2007-08-14 2016-09-13 Fluidigm Corporation Polymer microfluidic biochip fabrication
US20110126929A1 (en) 2007-08-15 2011-06-02 Massachusetts Institute Of Technology Microstructures For Fluidic Ballasting and Flow Control
US9598737B2 (en) 2012-05-09 2017-03-21 Longhorn Vaccines And Diagnostics, Llc Next generation genomic sequencing methods
US20090053704A1 (en) 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20100256017A1 (en) 2007-09-17 2010-10-07 Harry Benjamin Larman Supramolecular nanostamping printing device
US7790387B2 (en) 2007-09-24 2010-09-07 Agilent Technologies, Inc. Thiocarbonate linkers for polynucleotides
CA2700838A1 (en) 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
WO2009070665A1 (en) 2007-11-27 2009-06-04 Massachusetts Institute Of Technology Near field detector for integrated surface plasmon resonance biosensor applications
US20100272711A1 (en) 2007-12-12 2010-10-28 Thomas Jefferson University Compositions and methods for the treatment and prevention of cardiovascular diseases
EP2247708A4 (en) 2007-12-17 2013-02-27 Yeda Res & Dev SYSTEM AND METHOD FOR PROCESSING AND MANIPULATING DNA
WO2012044847A1 (en) 2010-10-01 2012-04-05 Life Technologies Corporation Nucleic acid adaptors and uses thereof
CN102016068A (zh) 2008-01-09 2011-04-13 生命科技公司 制备用于核酸测序的配对标签文库的方法
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
WO2009092564A2 (en) 2008-01-23 2009-07-30 Roche Diagnostics Gmbh Integrated instrument performing synthesis and amplification
WO2009131724A2 (en) 2008-01-24 2009-10-29 Massachusetts Institute Of Technology Insulated nanogap devices and methods of use thereof
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US20090194483A1 (en) 2008-01-31 2009-08-06 Robotti Karla M Microfluidic device having monolithic separation medium and method of use
US8968999B2 (en) 2008-02-15 2015-03-03 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
WO2009113709A1 (ja) 2008-03-11 2009-09-17 国立大学法人東京大学 粘着末端を有するｄｎａ断片の調製方法
US20090230044A1 (en) 2008-03-13 2009-09-17 Agilent Technologies, Inc. Microfluid Chip Cleaning
US20090238722A1 (en) 2008-03-18 2009-09-24 Agilent Technologies, Inc. Pressure-Reinforced Fluidic Chip
EP2286217B1 (en) 2008-03-31 2015-02-18 Pacific Biosciences of California, Inc. Single molecule loading methods and compositions
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
JP4582224B2 (ja) * 2008-05-02 2010-11-17 ソニー株式会社 マイクロビーズ作製方法及びマイクロビーズ
EP3733871A1 (en) 2008-05-27 2020-11-04 Dako Denmark A/S Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers
CN102164962B (zh) 2008-06-30 2014-05-28 诺福泰克公司 抗gd2抗体和方法及其相关应用
GB2461546B (en) 2008-07-02 2010-07-07 Argen X Bv Antigen binding polypeptides
JP4667490B2 (ja) 2008-07-09 2011-04-13 三菱電機株式会社 加熱調理器
WO2010014903A1 (en) 2008-07-31 2010-02-04 Massachusetts Institute Of Technology Multiplexed olfactory receptor-based microsurface plasmon polariton detector
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
SG191561A1 (en) 2008-08-22 2013-07-31 Sangamo Biosciences Inc Methods and compositions for targeted single-stranded cleavage and targeted integration
US8808986B2 (en) * 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
US8034917B2 (en) 2008-08-28 2011-10-11 Agilent Technologies, Inc. Primer-directed chromosome painting
CN102159726A (zh) 2008-09-05 2011-08-17 生命科技公司 用于核酸测序验证、校准和标准化的方法和系统
EP2334692B1 (en) 2008-09-05 2016-04-13 The Royal Institution for the Advancement of Learning/McGill University Rna monomers containing o-acetal levulinyl ester groups and their use in rna microarrays
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US8541569B2 (en) 2008-09-06 2013-09-24 Chemgenes Corporation Phosphoramidites for synthetic RNA in the reverse direction, efficient RNA synthesis and convenient introduction of 3'-end ligands, chromophores and modifications of synthetic RNA
AU2009288632B2 (en) 2008-09-06 2015-08-13 Chemgenes Corporation RNA synthesis - phosphoramidites for synthetic RNA in the reverse direction, and application in convenient introduction of ligands, chromophores and modifications of synthetic RNA at the 3' - end
CN102124112B (zh) 2008-09-10 2013-03-20 金斯瑞公司 基于同源重组dna的克隆方法及组合物
US20100076183A1 (en) 2008-09-22 2010-03-25 Dellinger Douglas J Protected monomer and method of final deprotection for rna synthesis
US8213015B2 (en) 2008-09-25 2012-07-03 Agilent Technologies, Inc. Integrated flow cell with semiconductor oxide tubing
JP2012504416A (ja) 2008-09-30 2012-02-23 アボット・ラボラトリーズ 改良抗体ライブラリー
US20100090341A1 (en) 2008-10-14 2010-04-15 Molecular Imprints, Inc. Nano-patterned active layers formed by nano-imprint lithography
US20100301398A1 (en) * 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010053443A1 (en) * 2008-11-06 2010-05-14 Agency For Science, Technology And Research Apparatus for biopolymer synthesis
US8357489B2 (en) 2008-11-13 2013-01-22 The Board Of Trustees Of The Leland Stanford Junior University Methods for detecting hepatocellular carcinoma
US20110229471A1 (en) 2008-11-26 2011-09-22 Cedars-Sinai Medical Center Methods of determining responsiveness to anti-tnf alpha therapy in inflammatory bowel disease
US9394333B2 (en) 2008-12-02 2016-07-19 Wave Life Sciences Japan Method for the synthesis of phosphorus atom modified nucleic acids
US8963262B2 (en) 2009-08-07 2015-02-24 Massachusettes Institute Of Technology Method and apparatus for forming MEMS device
JO3382B1 (ar) 2008-12-23 2019-03-13 Amgen Inc أجسام مضادة ترتبط مع مستقبل cgrp بشري
TW201104253A (en) 2008-12-31 2011-02-01 Nat Health Research Institutes Microarray chip and method of fabricating for the same
EP2393833A1 (en) 2009-02-09 2011-12-14 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Repertoire of allo-restricted peptide-specific t cell receptor sequences and use thereof
US8569046B2 (en) 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels
WO2010094772A1 (en) * 2009-02-20 2010-08-26 Febit Holding Gmbh Synthesis of sequence-verified nucleic acids
CN105132395A (zh) 2009-03-09 2015-12-09 生物蛋白有限公司 Mirac蛋白
WO2010115122A2 (en) * 2009-04-03 2010-10-07 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
US7862716B2 (en) 2009-04-13 2011-01-04 Sielc Technologies Corporation HPLC schematic with integrated sample cleaning system
EP2424669B1 (en) 2009-04-29 2020-06-03 Sicpa Holding Sa Method for depositing a biological fluid onto a substrate
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US20100292102A1 (en) 2009-05-14 2010-11-18 Ali Nouri System and Method For Preventing Synthesis of Dangerous Biological Sequences
US20100300882A1 (en) 2009-05-26 2010-12-02 General Electric Company Devices and methods for in-line sample preparation of materials
DK2438195T3 (en) 2009-06-02 2015-03-23 Univ California Tracking of virus by sequencing and assembly of virus-derived siRNAs, miRNAs and piRNA'er
WO2010141249A2 (en) 2009-06-02 2010-12-09 Merck Sharp & Dohme Corp. Generation, characterization and uses thereof of anti-notch3 antibodies
TWM370169U (en) 2009-06-10 2009-12-01 Wistron Corp Push button component with illumination structure and electronic device
US8309710B2 (en) 2009-06-29 2012-11-13 Agilent Technologies, Inc. Use of N-alkyl imidazole for sulfurization of oligonucleotides with an acetyl disulfide
US8642755B2 (en) 2009-06-30 2014-02-04 Agilent Technologies, Inc. Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
US8329208B2 (en) 2009-07-28 2012-12-11 Methylation Sciences International Srl Pharmacokinetics of S-adenosylmethionine formulations
CA2769302C (en) 2009-07-30 2018-10-16 F. Hoffmann-La Roche Ag A set of oligonucleotide probes as well as methods and uses related thereto
AU2010285265B2 (en) 2009-08-19 2015-05-21 Merck Patent Gmbh Antibodies for the detection of integrin complexes in FFPE material
ES2562159T3 (es) 2009-08-20 2016-03-02 Population Genetics Technologies Ltd. Composiciones y métodos para el reordenamiento de ácido nucleico molecular
US8476598B1 (en) 2009-08-31 2013-07-02 Sionyx, Inc. Electromagnetic radiation imaging devices and associated methods
US20110082055A1 (en) 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
US20120184724A1 (en) 2009-09-22 2012-07-19 Agilent Technologies, Inc. Protected monomers and methods of deprotection for rna synthesis
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
US8975019B2 (en) 2009-10-19 2015-03-10 University Of Massachusetts Deducing exon connectivity by RNA-templated DNA ligation/sequencing
US8389689B2 (en) 2009-10-28 2013-03-05 Janssen Biotech, Inc. Anti-GLP-1R antibodies and their uses
US20120315670A1 (en) 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US20110114549A1 (en) 2009-11-13 2011-05-19 Agilent Technolgies, Inc. Microfluidic device comprising separation columns
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8500979B2 (en) 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9758817B2 (en) 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
KR101230350B1 (ko) 2010-01-27 2013-02-06 주식회사 엘지화학 구조적 안정성이 우수한 전지팩
KR20110094878A (ko) 2010-02-18 2011-08-24 삼성전자주식회사 올리고머 어레이 제조용 조성물 및 올리고머 어레이 제조 방법
US20120027786A1 (en) 2010-02-23 2012-02-02 Massachusetts Institute Of Technology Genetically programmable pathogen sense and destroy
GB201003036D0 (en) 2010-02-23 2010-04-07 Fermentas Uab Restriction endonucleases and their applications
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
CA2792676A1 (en) 2010-03-09 2011-09-15 Toxcure, Inc. Microneedle nasal delivery device
JP5863766B2 (ja) 2010-04-09 2016-02-17 ザ・キャソリック・ユニバーシティ・オブ・アメリカＴｈｅ Ｃａｔｈｏｌｉｃ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ａｍｅｒｉｃａ タンパク質および核酸送達媒体、その成分および機構
US9125790B2 (en) 2010-04-12 2015-09-08 Mayo Foundation For Medical Education And Research System and method for alleviating freezing gait and gait hypokinesia in users with extrapyramidal disorder
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
DE102010027122A1 (de) * 2010-07-14 2012-01-19 Airbus Operations Gmbh Aufenthaltsmodul mit separatem Privatbereich
EP2598528A1 (en) 2010-07-28 2013-06-05 Immunocore Ltd. T cell receptors
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012045001A2 (en) 2010-09-30 2012-04-05 Vanderbilt University Influenza virus antibodies and immunogens and uses therefor
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
EP2630264A4 (en) * 2010-10-22 2014-04-02 Harvard College Orthogonal amplification and assembly of nucleic acid sequences
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
US20120164633A1 (en) 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Digital droplet sequencing
US9487807B2 (en) 2010-12-27 2016-11-08 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular DNA
MX344595B (es) 2010-12-31 2016-12-20 Bioatla Llc Humanizacion rapida de anticuerpos.
CN103597068A (zh) 2011-03-30 2014-02-19 独立行政法人国立长寿医疗研究中心 膜分选培养器、膜分选培养试剂盒、和使用其的干细胞分选方法、以及分离膜
US10131903B2 (en) 2011-04-01 2018-11-20 The Regents Of The University Of California Microfluidic platform for synthetic biology applications
US9384920B1 (en) 2011-04-04 2016-07-05 Eric J. Bakulich Locking knob
WO2012149171A1 (en) 2011-04-27 2012-11-01 The Regents Of The University Of California Designing padlock probes for targeted genomic sequencing
US8722585B2 (en) 2011-05-08 2014-05-13 Yan Wang Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
US10251611B2 (en) 2011-06-10 2019-04-09 Bright Devices Group Pty Ltd Freezing of gait cue apparatus
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
PT2723764T (pt) 2011-06-21 2018-03-09 Univ Leland Stanford Junior Domínios de ligação dirigidos contra complexos gpcr:proteína g e suas utilizações derivadas
CA2840542A1 (en) 2011-06-28 2013-01-03 Igor Kutyavin Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US20130045483A1 (en) 2011-07-01 2013-02-21 Whitehead Institute For Biomedical Research Yeast cells expressing amyloid beta and uses therefor
US9139874B2 (en) 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
WO2013010062A2 (en) 2011-07-14 2013-01-17 Life Technologies Corporation Nucleic acid complexity reduction
EP2748318B1 (en) 2011-08-26 2015-11-04 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US20150203839A1 (en) 2011-08-26 2015-07-23 Gen9, Inc. Compositions and Methods for High Fidelity Assembly of Nucleic Acids
US20150120265A1 (en) 2011-09-01 2015-04-30 Genome Compiler Corporation System for polynucleotide construct design, visualization and transactions to manufacture the same
WO2013036668A1 (en) 2011-09-06 2013-03-14 Gen-Probe Incorporated Circularized templates for sequencing
US8840981B2 (en) * 2011-09-09 2014-09-23 Eastman Kodak Company Microfluidic device with multilayer coating
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2013055822A2 (en) 2011-10-11 2013-04-18 Life Technologies Corporation Systems and methods for analysis and interpretation of nucleic acid sequence data
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US8987174B2 (en) 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
US8815782B2 (en) 2011-11-11 2014-08-26 Agilent Technologies, Inc. Use of DNAzymes for analysis of an RNA sample
US8450107B1 (en) 2011-11-30 2013-05-28 The Broad Institute Inc. Nucleotide-specific recognition sequences for designer TAL effectors
US20130137173A1 (en) 2011-11-30 2013-05-30 Feng Zhang Nucleotide-specific recognition sequences for designer tal effectors
EP2599785A1 (en) 2011-11-30 2013-06-05 Agilent Technologies, Inc. Novel methods for the synthesis and purification of oligomers
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
WO2013093693A1 (en) 2011-12-22 2013-06-27 Rinat Neuroscience Corp. Staphylococcus aureus specific antibodies and uses thereof
EP3495499A1 (en) 2011-12-30 2019-06-12 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
EP2809795B1 (en) 2012-02-01 2019-09-18 SGI-DNA, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
EP2825653A4 (en) 2012-03-14 2016-01-20 Innovative Targeting Solutions Inc PRODUCTION OF TARGETED SEQUENCE DIVERSITY IN FUSION PROTEINS
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
JP6238417B2 (ja) 2012-03-28 2017-11-29 ケーシーアイ ライセンシング インコーポレイテッド 電子構成部分と臨床構成部分の分離を促進する減圧システム、ドレッシング、および方法
US9732384B2 (en) 2012-04-02 2017-08-15 Lux Bio Group, Inc. Apparatus and method for molecular separation, purification, and sensing
JP6301311B2 (ja) 2012-04-10 2018-03-28 ザ トラスティーズ オブ プリンストン ユニバーシティＴｈｅ Ｔｒｕｓｔｅｅｓ Ｏｆ Ｐｒｉｎｃｅｔｏｎ Ｕｎｉｖｅｒｓｉｔｙ 超高感度センサ
US20150353921A9 (en) 2012-04-16 2015-12-10 Jingdong Tian Method of on-chip nucleic acid molecule synthesis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
US20130281308A1 (en) 2012-04-24 2013-10-24 Gen9, Inc. Methods for sorting nucleic acids and preparative in vitro cloning
WO2015066174A1 (en) 2013-10-29 2015-05-07 Longhorn Vaccines And Diagnostics, Llc Next generation genomic sequencing methods
CA2872908C (en) 2012-05-10 2023-11-14 Gerhard Frey Multi-specific monoclonal antibodies
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US10308979B2 (en) 2012-06-01 2019-06-04 Agilent Technologies, Inc. Target enrichment and labeling for multi-kilobase DNA
DK2856375T3 (en) 2012-06-01 2018-11-05 European Molecular Biology Laboratory High capacity storage of digital information in DNA
US9102936B2 (en) 2012-06-11 2015-08-11 Agilent Technologies, Inc. Method of adaptor-dimer subtraction using a CRISPR CAS6 protein
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
ES2769796T3 (es) 2012-07-03 2020-06-29 Integrated Dna Tech Inc Oligonucleótidos de bloqueo aumentados en Tm y señuelos para un enriquecimiento de diana mejorado y una selección fuera de diana reducida
US20140038240A1 (en) 2012-07-10 2014-02-06 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
US9073962B2 (en) 2012-07-12 2015-07-07 Massachusetts Institute Of Technology Methods of serial assembly of DNA bricks into larger structures
JP6239813B2 (ja) 2012-07-18 2017-11-29 株式会社Ｓｃｒｅｅｎセミコンダクターソリューションズ 基板処理装置および基板処理方法
CN108875312A (zh) 2012-07-19 2018-11-23 哈佛大学校长及研究员协会 利用核酸存储信息的方法
CN104684926B (zh) 2012-07-27 2022-04-08 伊利诺伊州大学理事会 工程化t细胞受体
WO2014021938A1 (en) 2012-08-02 2014-02-06 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization
SG11201500724QA (en) 2012-08-16 2015-02-27 Synthetic Genomics Inc Digital to biological converter
WO2014035693A2 (en) 2012-08-31 2014-03-06 The Scripps Research Institute Methods and compositions related to modulators of eukaryotic cells
WO2014039587A1 (en) 2012-09-05 2014-03-13 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
WO2014062717A1 (en) 2012-10-15 2014-04-24 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
KR20140048733A (ko) * 2012-10-16 2014-04-24 삼성전자주식회사 다중 웰 플레이트 및 상기 다중 웰 플레이트를 이용한 표적 물질 분석 방법
WO2014066179A1 (en) 2012-10-24 2014-05-01 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
EP2928500B1 (en) 2012-12-04 2019-03-06 Phosphorex Inc. Microparticles and nanoparticles having negative surface charges
JP6745599B2 (ja) 2012-12-06 2020-08-26 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 分子の作製
CN104854246B (zh) 2012-12-06 2018-05-01 安捷伦科技有限公司 无限制酶的靶标富集
US9976162B2 (en) 2012-12-10 2018-05-22 Agilent Technologies, Inc. Pairing code directed assembly
HK1213944A1 (zh) 2012-12-10 2016-07-15 Resolution Bioscience, Inc. 用於靶向基因组分析
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
SG10201707011PA (en) 2013-02-28 2017-10-30 Univ Nanyang Tech Method of manufacturing a device for supporting biological material growth and device therefrom
WO2014137328A1 (en) 2013-03-05 2014-09-12 Agilent Technologies, Inc. Detection of genomic rearrangements by sequence capture
US9580746B2 (en) 2013-03-05 2017-02-28 Agilent Technologies, Inc. Synthesis of long fish probes
WO2014160059A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
EP2971034B1 (en) 2013-03-13 2020-12-02 Gen9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
US20140328849A1 (en) 2013-03-15 2014-11-06 Genentech, Inc. Anti-crth2 antibodies and methods of use
US20140274741A1 (en) 2013-03-15 2014-09-18 The Translational Genomics Research Institute Methods to capture and sequence large fragments of dna and diagnostic methods for neuromuscular disease
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
WO2014151696A1 (en) 2013-03-15 2014-09-25 Gen9, Inc. Compositions and methods for multiplex nucleic acids synthesis
EP3795696B1 (en) 2013-03-15 2023-04-26 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US20150293102A1 (en) 2013-04-13 2015-10-15 Jung-Uk Shim Detecting low-abundant analyte in microfluidic droplets
ITRM20130278A1 (it) 2013-05-10 2014-11-11 Consiglio Nazionale Ricerche Procedimento di fabbricazione di film autoassemblati di copolimeri a blocchi
CA2916960C (en) 2013-06-26 2022-12-06 Guangzhou Xiangxue Pharmaceutical Co. Ltd High-stability t-cell receptor and preparation method and application thereof
US20150010953A1 (en) 2013-07-03 2015-01-08 Agilent Technologies, Inc. Method for producing a population of oligonucleotides that has reduced synthesis errors
KR20150005062A (ko) 2013-07-04 2015-01-14 삼성전자주식회사 미니-코어를 사용하는 프로세서
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
EP3027771B1 (en) 2013-07-30 2019-01-16 Gen9, Inc. Methods for the production of long length clonal sequence verified nucleic acid constructs
EP4610368B1 (en) 2013-08-05 2026-02-04 Twist Bioscience Corporation De novo synthesized gene libraries
US9589445B2 (en) 2013-08-07 2017-03-07 Nike, Inc. Activity recognition with activity reminders
CN104371019B (zh) 2013-08-13 2019-09-10 鸿运华宁（杭州）生物医药有限公司 一种能与glp-1r特异性结合的抗体及其与glp-1的融合蛋白质
GB201314721D0 (en) 2013-08-16 2013-10-02 Almagen Ltd A method of selectively masking one or more sites on a surface and a method of synthesising an array of molecules
WO2015023976A2 (en) * 2013-08-16 2015-02-19 Memorial Sloan-Kettering Cancer Center Selective grp94 inhibitors and uses thereof
WO2015031689A1 (en) 2013-08-30 2015-03-05 Personalis, Inc. Methods and systems for genomic analysis
CN105916873B (zh) 2013-09-14 2020-06-19 坎姆根公司 使用反向法高效合成长rna
WO2015040075A1 (en) 2013-09-18 2015-03-26 Genome Research Limited Genomic screening methods using rna-guided endonucleases
US9311311B2 (en) 2013-09-27 2016-04-12 International Business Machines Corporation Archival management of database logs
US9422325B2 (en) 2013-10-04 2016-08-23 Trustees Of Tufts College Glycosylation reactions using phenyl(trifluoroethyl)iodonium salts
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
CA2963072A1 (en) 2013-10-29 2015-05-07 Milbat - Giving Quality To Life Walker-assist device
BR112016012164A2 (pt) 2013-11-26 2017-09-26 Xenco Medical LLC sistema de liberação de implante
CN105934541B (zh) 2013-11-27 2019-07-12 Gen9股份有限公司 核酸文库及其制造方法
KR102454360B1 (ko) 2013-12-04 2022-10-12 추가이 세이야쿠 가부시키가이샤 화합물의 농도에 따라 항원 결합능이 변화되는 항원 결합 분자 및 그의 라이브러리
US20160297883A1 (en) 2013-12-04 2016-10-13 Innovative Targeting Solutions, Inc. G-protein coupled receptor agonists and methods
CA2945628A1 (en) 2013-12-09 2015-06-18 Integrated Dna Technologies, Inc. Long nuceic acid sequences containing variable regions
NZ760065A (en) 2013-12-17 2022-12-23 Genentech Inc Methods of treating cancers using pd-1 axis binding antagonists and taxanes
GB2521387B (en) 2013-12-18 2020-05-27 Ge Healthcare Uk Ltd Oligonucleotide data storage on solid supports
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
WO2015120403A1 (en) 2014-02-08 2015-08-13 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking pcr
WO2015134552A1 (en) 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
BR112016021004A2 (pt) 2014-03-14 2018-01-23 Immunocore Limited biblioteca de partículas; receptor de células t (tcr) isolado não natural; uso de uma biblioteca; método de obtenção de um receptor de células t que se liga especificamente a um antígeno de peptídeo; ácido nucleico; método de construção de uma biblioteca de partículas; método de obtenção de um receptor de células t que se liga especificamente a um antígeno de peptídeo; e partícula
WO2015195178A2 (en) 2014-03-27 2015-12-23 Canon U.S. Life Sciences, Inc. Integration of ex situ fabricated porous polymer monoliths into fluidic chips
US10190161B2 (en) 2014-04-03 2019-01-29 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanowire detectors
EP3133212A1 (en) 2014-04-15 2017-02-22 Volvo Construction Equipment AB Device for controlling engine and hydraulic pump of construction equipment and control method therefor
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
EP3143161B1 (en) 2014-05-16 2021-04-21 Illumina, Inc. Nucleic acid synthesis techniques
US20150361422A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
US20150361423A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US10472620B2 (en) 2014-07-01 2019-11-12 General Electric Company Method, substrate and device for separating nucleic acids
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CA2954791C (en) 2014-07-14 2025-11-18 The Regents Of The University Of California CRISPR/CAS TRANSCRIPTIONAL MODULATION
EP3169781B1 (en) 2014-07-15 2020-04-08 Life Technologies Corporation Compositions and methods for nucleic acid assembly
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
ES2880335T3 (es) 2014-09-09 2021-11-24 Igenomx Int Genomics Corporation Métodos y composiciones para la preparación rápida de bibliotecas de ácidos nucleicos
WO2016053883A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
US9879283B2 (en) 2014-10-09 2018-01-30 Life Technologies Corporation CRISPR oligonucleotides and gene editing
WO2016055956A1 (en) 2014-10-10 2016-04-14 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
WO2016059610A1 (en) 2014-10-18 2016-04-21 Malik Girik A biomolecule based data storage system
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
US9890417B2 (en) 2014-11-03 2018-02-13 Agilent Technologies, Inc. Signal amplification of fluorescence in situ hybridization
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
CN104562213A (zh) 2014-12-26 2015-04-29 北京诺禾致源生物信息科技有限公司 扩增子文库及其构建方法
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9834774B2 (en) 2015-02-11 2017-12-05 Agilent Technologies, Inc. Methods and compositions for rapid seamless DNA assembly
WO2016130868A2 (en) 2015-02-13 2016-08-18 Vaccine Research Institute Of San Diego Materials and methods to analyze rna isoforms in transcriptomes
CN104734848A (zh) 2015-03-02 2015-06-24 郑州轻工业学院 基于重组dna技术对信息进行加密与隐藏的方法及应用
CA2981517A1 (en) 2015-04-01 2016-10-06 The Scripps Research Institute Methods and compositions related to gpcr agonist polypeptides
WO2016162127A1 (en) 2015-04-08 2016-10-13 Polyphor Ag Backbone-cyclized peptidomimetics
US11164661B2 (en) 2015-04-10 2021-11-02 University Of Washington Integrated system for nucleic acid-based storage and retrieval of digital data using keys
US10125198B2 (en) 2015-04-17 2018-11-13 Distributed Bio, Inc. Method for mass humanization of non-human antibodies
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016173719A1 (en) 2015-04-30 2016-11-03 Abcheck S.R.O. Method for mass humanization of rabbit antibodies
US20160333340A1 (en) 2015-05-11 2016-11-17 Twist Bioscience Corporation Compositions and methods for nucleic acid amplification
US9928869B2 (en) 2015-07-13 2018-03-27 President And Fellows Of Harvard College Methods for retrievable information storage using nucleic acids
GB201513113D0 (en) 2015-07-24 2015-09-09 Genome Res Ltd Nasal sampling methods
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods for modulating protein and cellular activity and method for nucleic acid synthesis
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN108026137A (zh) 2015-10-01 2018-05-11 华盛顿大学 Dna寡核苷酸的多对组装
US20170141793A1 (en) 2015-11-13 2017-05-18 Microsoft Technology Licensing, Llc Error correction for nucleotide data stores
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
PT3387152T (pt) 2015-12-08 2022-04-19 Twinstrand Biosciences Inc Adaptadores, métodos e composições melhorados para sequenciamento duplex
JP7308505B2 (ja) 2016-01-08 2023-07-14 マキシオン セラピューティクス リミテッド 多様性を変化させた足場ドメインを有する結合メンバー
GB201604492D0 (en) 2016-03-16 2016-04-27 Immatics Biotechnologies Gmbh Transfected t-cells and t-cell receptors for use in immunotherapy against cancers
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
CN109564769A (zh) 2016-06-10 2019-04-02 特韦斯特生物科学公司 用于自动注释和筛选生物序列的系统和方法
CN110088281A (zh) 2016-08-03 2019-08-02 特韦斯特生物科学公司 用于多核苷酸合成的纹理化表面
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
CN109843922B (zh) 2016-09-02 2023-10-03 莱蒂恩技术公司 用DuoCAR治疗癌症的组合物和方法
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
JP2020504709A (ja) 2016-11-18 2020-02-13 ツイスト バイオサイエンス コーポレーション 制御された化学量論を有するポリヌクレオチドライブラリおよびその合成
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
PL3558369T3 (pl) 2016-12-21 2025-06-23 Cephalon Llc Przeciwciała specyficznie wiążące się z ludzką il-15 i ich zastosowania
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3056386A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
MY197427A (en) 2017-03-23 2023-06-16 QBiotics Pty Ltd Combination therapy for the treatment or prevention of tumours
WO2018183918A1 (en) 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
SG11201909918XA (en) 2017-04-23 2019-11-28 Illumina Cambridge Ltd Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP4443436A3 (en) 2017-05-08 2024-12-25 Illumina, Inc. Universal short adapters for indexing of polynucleotide
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566209B (zh) 2017-06-12 2024-08-30 特韦斯特生物科学公司 无缝核酸装配方法
US11666863B2 (en) 2017-07-18 2023-06-06 Investigaciones Forestales Bioforest S.A. Method and device for asymmetric polarity inversion in electromembrane processes
EP3681906A4 (en) 2017-09-11 2021-06-09 Twist Bioscience Corporation GPCR-BINDING PROTEINS AND THEIR SYNTHESIS
KR102889470B1 (ko) 2017-10-20 2025-11-21 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US20200299684A1 (en) 2017-10-27 2020-09-24 Twist Bioscience Corporation Systems and methods for polynucleotide scoring
US11427867B2 (en) 2017-11-29 2022-08-30 Xgenomes Corp. Sequencing by emergence
CA3088911A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based storage device and method for synthesizing polynucleotides using the device
US10722916B2 (en) 2018-01-19 2020-07-28 Caulk Garbage Can LLC Caulk gun attachment for wiping excess caulk
WO2019222706A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
US10963953B2 (en) 2018-10-10 2021-03-30 Alliance Inspection Management, LLC Reserve management for continuous bidding portal
US10969965B2 (en) 2018-12-24 2021-04-06 Western Digital Technologies, Inc. Dynamic performance density tuning for data storage device
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
EP3938505A4 (en) 2019-02-25 2022-11-30 Twist Bioscience Corporation COMPOSITIONS AND METHODS FOR SEQUENCING THE NEXT GENERATION
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US20220243195A1 (en) 2019-06-21 2022-08-04 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CA3148245A1 (en) 2019-07-22 2021-01-28 Igenomx International Genomics Corporation Methods and compositions for high throughput sample preparation using double unique dual indexing
CA3154235A1 (en) 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
EP4142739A2 (en) 2020-04-27 2023-03-08 Twist Bioscience Corporation Variant nucleic acid libraries for coronavirus
CN116472302A (zh) 2020-07-07 2023-07-21 特韦斯特生物科学公司 用于光引导聚合物合成的装置和方法
US20220106586A1 (en) 2020-08-25 2022-04-07 Twist Bioscience Corporation Compositions and methods for library sequencing
CA3190667A1 (en) 2020-08-26 2022-03-03 Aaron Sato Methods and compositions relating to glp1r variants
CA3190917A1 (en) 2020-08-28 2022-03-03 Andres Fernandez Devices and methods for synthesis
WO2022076326A1 (en) 2020-10-05 2022-04-14 Twist Bioscience Corporation Hybridization methods and reagents
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
WO2022087293A1 (en) 2020-10-22 2022-04-28 Twist Bioscience Corporation Methods and systems for detecting coronavirus
US20220135965A1 (en) 2020-10-26 2022-05-05 Twist Bioscience Corporation Libraries for next generation sequencing
US20220135690A1 (en) 2020-11-03 2022-05-05 Twist Bioscience Corporation Methods and compositions relating to chemokine receptor variants
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
US20220277808A1 (en) 2021-02-19 2022-09-01 Twist Bioscience Corporation Libraries for identification of genomic variants
US20220323924A1 (en) 2021-03-24 2022-10-13 Twist Bioscience Corporation Electrochemical polynucleotide synthesis
EP4314074A4 (en) 2021-03-24 2025-09-03 Twist Bioscience Corp NUCLEIC ACID VARIANT BANKS FOR TIGIT
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
CA3214947A1 (en) 2021-04-09 2022-10-13 Twist Bioscience Corporation Libraries for mutational analysis
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
EP4334516A1 (en) 2021-05-03 2024-03-13 Twist Bioscience Corporation Variant nucleic acid libraries for ion channels

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