DK3030682T3 - De novo synthesized gene libraries - Google Patents

De novo synthesized gene libraries Download PDF

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Publication number
DK3030682T3
DK3030682T3 DK14834665.3T DK14834665T DK3030682T3 DK 3030682 T3 DK3030682 T3 DK 3030682T3 DK 14834665 T DK14834665 T DK 14834665T DK 3030682 T3 DK3030682 T3 DK 3030682T3
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Prior art keywords
gene libraries
synthesized gene
novo synthesized
novo
libraries
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DK14834665.3T
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Danish (da)
English (en)
Inventor
William Banyai
Bill James Peck
Pierre Indermuhle
Andres Fernandez
Siyuan Chen
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Twist Bioscience Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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    • B01J2219/00718—Type of compounds synthesised
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DK14834665.3T 2013-08-05 2014-08-05 De novo synthesized gene libraries DK3030682T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361862445P 2013-08-05 2013-08-05
US201361862457P 2013-08-05 2013-08-05
PCT/US2014/049834 WO2015021080A2 (en) 2013-08-05 2014-08-05 De novo synthesized gene libraries

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DK3030682T3 true DK3030682T3 (da) 2020-09-14

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US (20) US9409139B2 (enExample)
EP (4) EP4242321B1 (enExample)
JP (4) JP6656733B2 (enExample)
KR (6) KR102207770B1 (enExample)
CN (2) CN105637097A (enExample)
CA (1) CA2918258A1 (enExample)
DK (1) DK3030682T3 (enExample)
EA (1) EA034459B1 (enExample)
ES (2) ES2815099T3 (enExample)
GB (1) GB2533173A (enExample)
PL (1) PL3030682T3 (enExample)
SG (1) SG11201600853UA (enExample)
TW (5) TWI805996B (enExample)
WO (1) WO2015021080A2 (enExample)

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CA3002886A1 (en) * 2015-10-21 2017-04-27 F. Hoffmann-La Roche Ag Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore based dna sequencing
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
CN109310784B (zh) 2015-12-07 2022-08-19 阿克生物公司 用于制备和使用指导核酸的方法和组合物
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