EA034459B1 - Синтезированные de novo библиотеки генов - Google Patents

Синтезированные de novo библиотеки генов Download PDF

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Publication number
EA034459B1
EA034459B1 EA201690081A EA201690081A EA034459B1 EA 034459 B1 EA034459 B1 EA 034459B1 EA 201690081 A EA201690081 A EA 201690081A EA 201690081 A EA201690081 A EA 201690081A EA 034459 B1 EA034459 B1 EA 034459B1
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Eurasian Patent Office
Prior art keywords
substrate
less
loci
genes
nucleic acids
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EA201690081A
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English (en)
Russian (ru)
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EA201690081A1 (ru
Inventor
Уильям Баниайи
Билл Джеймс Пек
Андрес Фернандез
Сыюань Чэнь
Пьер Индермуле
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Твист Байосайенс Корпорейшн
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Publication of EA201690081A1 publication Critical patent/EA201690081A1/ru
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
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  • General Chemical & Material Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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EA201690081A 2013-08-05 2014-08-05 Синтезированные de novo библиотеки генов EA034459B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361862445P 2013-08-05 2013-08-05
US201361862457P 2013-08-05 2013-08-05
PCT/US2014/049834 WO2015021080A2 (en) 2013-08-05 2014-08-05 De novo synthesized gene libraries

Publications (2)

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EA201690081A1 EA201690081A1 (ru) 2016-10-31
EA034459B1 true EA034459B1 (ru) 2020-02-11

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EA201690081A EA034459B1 (ru) 2013-08-05 2014-08-05 Синтезированные de novo библиотеки генов

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US (20) US9409139B2 (enExample)
EP (4) EP4610368A3 (enExample)
JP (4) JP6656733B2 (enExample)
KR (6) KR102291045B1 (enExample)
CN (2) CN111593414A (enExample)
CA (1) CA2918258A1 (enExample)
DK (1) DK3030682T3 (enExample)
EA (1) EA034459B1 (enExample)
ES (2) ES2943498T3 (enExample)
GB (1) GB2533173A (enExample)
PL (1) PL3030682T3 (enExample)
SG (1) SG11201600853UA (enExample)
TW (5) TWI707038B (enExample)
WO (1) WO2015021080A2 (enExample)

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HK1258869A1 (zh) * 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
WO2017070549A1 (en) * 2015-10-21 2017-04-27 Genia Technologies, Inc. Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore
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