ES2815099T3 - Genotecas sintetizadas de novo - Google Patents

Genotecas sintetizadas de novo Download PDF

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Publication number
ES2815099T3
ES2815099T3 ES14834665T ES14834665T ES2815099T3 ES 2815099 T3 ES2815099 T3 ES 2815099T3 ES 14834665 T ES14834665 T ES 14834665T ES 14834665 T ES14834665 T ES 14834665T ES 2815099 T3 ES2815099 T3 ES 2815099T3
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Prior art keywords
examples
substrate
genes
resolved
oligonucleotides
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Spanish (es)
Inventor
William Banyai
Bill James Peck
Andres Fernandez
Siyuan Chen
Pierre Indermuhle
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Twist Bioscience Corp
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Twist Bioscience Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Structural Engineering (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
ES14834665T 2013-08-05 2014-08-05 Genotecas sintetizadas de novo Active ES2815099T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361862445P 2013-08-05 2013-08-05
US201361862457P 2013-08-05 2013-08-05
PCT/US2014/049834 WO2015021080A2 (en) 2013-08-05 2014-08-05 De novo synthesized gene libraries

Publications (1)

Publication Number Publication Date
ES2815099T3 true ES2815099T3 (es) 2021-03-29

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ES14834665T Active ES2815099T3 (es) 2013-08-05 2014-08-05 Genotecas sintetizadas de novo
ES20169965T Active ES2943498T3 (es) 2013-08-05 2014-08-05 Genotecas sintetizadas de novo

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US (20) US9409139B2 (enExample)
EP (4) EP3030682B1 (enExample)
JP (4) JP6656733B2 (enExample)
KR (6) KR102207770B1 (enExample)
CN (2) CN105637097A (enExample)
CA (1) CA2918258A1 (enExample)
DK (1) DK3030682T3 (enExample)
EA (1) EA034459B1 (enExample)
ES (2) ES2815099T3 (enExample)
GB (1) GB2533173A (enExample)
PL (1) PL3030682T3 (enExample)
SG (1) SG11201600853UA (enExample)
TW (5) TWI707038B (enExample)
WO (1) WO2015021080A2 (enExample)

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KR20190002733A (ko) 2010-12-30 2019-01-08 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
US9409139B2 (en) * 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries
EP3968023B1 (en) 2013-08-19 2025-10-08 Invitae Corporation Assays for single molecule detection and use thereof
SG10201911069WA (en) * 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
ES2989276T3 (es) 2014-12-05 2024-11-25 Found Medicine Inc Análisis multigénico de muestras tumorales
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corp Compositions and methods for synthetic gene assembly
CN113528623A (zh) * 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
JP6703599B2 (ja) * 2015-09-14 2020-06-03 エッセンリックス コーポレーション 試料、特に血液を分析するための装置及びシステム、並びにそれらの使用方法
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