DE69032621T2 - Zweiwellenlängenlaserabtastmikroskop - Google Patents

Zweiwellenlängenlaserabtastmikroskop

Info

Publication number
DE69032621T2
DE69032621T2 DE69032621T DE69032621T DE69032621T2 DE 69032621 T2 DE69032621 T2 DE 69032621T2 DE 69032621 T DE69032621 T DE 69032621T DE 69032621 T DE69032621 T DE 69032621T DE 69032621 T2 DE69032621 T2 DE 69032621T2
Authority
DE
Germany
Prior art keywords
excitation
wavelength laser
laser scan
scan microscope
submicrosecond
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69032621T
Other languages
English (en)
Other versions
DE69032621D1 (de
DE69032621T3 (de
Inventor
Winfried Denk
James Strickler
Watt Webb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cornell Research Foundation Inc
Original Assignee
Cornell Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23730872&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69032621(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cornell Research Foundation Inc filed Critical Cornell Research Foundation Inc
Publication of DE69032621D1 publication Critical patent/DE69032621D1/de
Application granted granted Critical
Publication of DE69032621T2 publication Critical patent/DE69032621T2/de
Publication of DE69032621T3 publication Critical patent/DE69032621T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B7/00—Recording or reproducing by optical means, e.g. recording using a thermal beam of optical radiation by modifying optical properties or the physical structure, reproducing using an optical beam at lower power by sensing optical properties; Record carriers therefor
    • G11B7/004—Recording, reproducing or erasing methods; Read, write or erase circuits therefor
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B7/00—Recording or reproducing by optical means, e.g. recording using a thermal beam of optical radiation by modifying optical properties or the physical structure, reproducing using an optical beam at lower power by sensing optical properties; Record carriers therefor
    • G11B7/12—Heads, e.g. forming of the optical beam spot or modulation of the optical beam
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B7/00—Recording or reproducing by optical means, e.g. recording using a thermal beam of optical radiation by modifying optical properties or the physical structure, reproducing using an optical beam at lower power by sensing optical properties; Record carriers therefor
    • G11B7/12—Heads, e.g. forming of the optical beam spot or modulation of the optical beam
    • G11B7/135—Means for guiding the beam from the source to the record carrier or from the record carrier to the detector
    • G11B7/1362—Mirrors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G01N2021/6415—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence with two excitations, e.g. strong pump/probe flash
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/631—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using photolysis and investigating photolysed fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
DE69032621T 1989-11-14 1990-11-13 Zweiwellenlängenlaserabtastmikroskop Expired - Lifetime DE69032621T3 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US436045 1989-11-14
US07/436,045 US5034613A (en) 1989-11-14 1989-11-14 Two-photon laser microscopy
PCT/US1990/006482 WO1991007651A1 (en) 1989-11-14 1990-11-13 Two-photon laser scanning microscopy

Publications (3)

Publication Number Publication Date
DE69032621D1 DE69032621D1 (de) 1998-10-08
DE69032621T2 true DE69032621T2 (de) 1999-03-25
DE69032621T3 DE69032621T3 (de) 2004-08-19

Family

ID=23730872

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69032621T Expired - Lifetime DE69032621T3 (de) 1989-11-14 1990-11-13 Zweiwellenlängenlaserabtastmikroskop
DE69034117T Expired - Lifetime DE69034117T2 (de) 1989-11-14 1990-11-13 Zwei-Photonen molekulare Anregung in einem Laserrastermikroskop

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69034117T Expired - Lifetime DE69034117T2 (de) 1989-11-14 1990-11-13 Zwei-Photonen molekulare Anregung in einem Laserrastermikroskop

Country Status (9)

Country Link
US (1) US5034613A (de)
EP (2) EP0807814B1 (de)
JP (1) JP2848952B2 (de)
AT (2) ATE170636T1 (de)
DE (2) DE69032621T3 (de)
DK (1) DK0807814T3 (de)
ES (2) ES2123501T5 (de)
HK (1) HK1010411A1 (de)
WO (1) WO1991007651A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007055530A1 (de) * 2007-11-21 2009-05-28 Carl Zeiss Ag Laserstrahlbearbeitung

Families Citing this family (354)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5260578A (en) * 1991-04-10 1993-11-09 Mayo Foundation For Medical Education And Research Confocal imaging system for visible and ultraviolet light
US6564087B1 (en) 1991-04-29 2003-05-13 Massachusetts Institute Of Technology Fiber optic needle probes for optical coherence tomography imaging
US6111645A (en) 1991-04-29 2000-08-29 Massachusetts Institute Of Technology Grating based phase control optical delay line
US6485413B1 (en) 1991-04-29 2002-11-26 The General Hospital Corporation Methods and apparatus for forward-directed optical scanning instruments
US5289407A (en) * 1991-07-22 1994-02-22 Cornell Research Foundation, Inc. Method for three dimensional optical data storage and retrieval
JP3082346B2 (ja) * 1991-09-12 2000-08-28 株式会社ニコン 蛍光コンフォーカル顕微鏡
US5487080A (en) * 1991-10-30 1996-01-23 University Of New Mexico Principle and applications of multiphoton pumped upconverted lasers
US5296703A (en) * 1992-04-01 1994-03-22 The Regents Of The University Of California Scanning confocal microscope using fluorescence detection
EP0627643B1 (de) * 1993-06-03 1999-05-06 Hamamatsu Photonics K.K. Optisches Laser-Abtastsystem mit Axikon
US5923430A (en) 1993-06-17 1999-07-13 Ultrapointe Corporation Method for characterizing defects on semiconductor wafers
USH1530H (en) * 1993-06-17 1996-05-07 Ultrapointe Corporation Surface extraction from a three-dimensional data set
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
DE4324681C2 (de) * 1993-07-22 1997-09-04 Hell Stefan Verfahren zur optischen Anregung eines Energiezustands einer Probe in einem Probenpunkt und Vorrichtung zur Durchführung des Verfahrens
DE4326181A1 (de) * 1993-08-04 1995-02-09 Europ Lab Molekularbiolog Verfahren und Vorrichtung zur Lumineszenzspektroskopie und Materialmikrobearbeitung von fixierten und bewegten Molekülen, Partikeln und Objekten
DE4331570C2 (de) * 1993-08-17 1996-10-24 Hell Stefan Verfahren zum optischen Anregen einer Probe
FI96452C (fi) * 1994-01-26 1996-06-25 Pekka Haenninen Menetelmä väriaineiden virittämiseksi
EP0801759B1 (de) * 1994-02-01 2001-08-08 Stefan Dr. Hell Vorrichtung und verfahren zum optischen messen eines probenpunktes einer probe mit hoher ortsauflösung
DE4414940C2 (de) * 1994-04-28 1998-07-02 Pekka Haenninen Lumineszenz-Rastermikroskop mit zwei Photonen Anregung
US5686988A (en) * 1994-06-28 1997-11-11 Lockheed Martin Energy Systems, Inc. Gas concentration measurement instrument based on the effects of a wave-mixing interference on stimulated emissions
US20050111089A1 (en) * 1994-07-15 2005-05-26 Baer Stephen C. Superresolving microscopy apparatus
US6259104B1 (en) 1994-07-15 2001-07-10 Stephen C. Baer Superresolution in optical microscopy and microlithography
US5952668A (en) * 1994-07-15 1999-09-14 Baer; Stephen C. Resolution in microscopy and microlithography
US6903347B2 (en) 1994-07-15 2005-06-07 Stephen C. Baer Superresolution in microlithography and fluorescence microscopy
US5866911A (en) * 1994-07-15 1999-02-02 Baer; Stephen C. Method and apparatus for improving resolution in scanned optical system
US7071477B2 (en) * 1994-07-15 2006-07-04 Baer Stephen C Superresolution in microlithography and fluorescence microscopy
US5710046A (en) * 1994-11-04 1998-01-20 Amoco Corporation Tagging hydrocarbons for subsequent identification
US5723338A (en) * 1994-11-04 1998-03-03 Amoco Corporation Tagging hydrocarbons for subsequent identification
US5843783A (en) * 1994-11-04 1998-12-01 Amoco Corporation Tagging hydrocarbons for subsequent identification
US5835262A (en) * 1994-12-28 1998-11-10 Research Development Corporation Of Japan Multi-wavelength optical microscope
DE69615818T2 (de) * 1995-01-16 2002-06-06 Erkki Soini Ein biospezifisches multiparametrisches testverfahren
FI101829B (fi) * 1995-03-07 1998-08-31 Erkki Juhani Soini Biospesifinen määritysmenetelmä
FI98765C (fi) * 1995-01-16 1997-08-11 Erkki Soini Virtaussytometrinen menetelmä ja laite
US5863504A (en) * 1995-03-16 1999-01-26 Bio-Rad Laboratories, Inc. Fluorescence imaging instrument utilizing fish
US5786560A (en) * 1995-03-31 1998-07-28 Panasonic Technologies, Inc. 3-dimensional micromachining with femtosecond laser pulses
US5813987A (en) * 1995-08-01 1998-09-29 Medispectra, Inc. Spectral volume microprobe for analysis of materials
US6104945A (en) * 1995-08-01 2000-08-15 Medispectra, Inc. Spectral volume microprobe arrays
US5713364A (en) * 1995-08-01 1998-02-03 Medispectra, Inc. Spectral volume microprobe analysis of materials
CA2231114A1 (en) * 1995-09-06 1997-03-13 The Research Foundation Of State University Of New York Two-photon upconverting dyes and applications
DE19533092A1 (de) * 1995-09-07 1997-03-13 Basf Ag Vorrichtung zur parallelisierten Zweiphotonen-Fluoreszenz-Korrelations-Spektroskopie (TPA-FCS) und deren Verwendung zum Wirkstoff-Screening
DE69635521T2 (de) * 1995-09-19 2006-08-17 Cornell Research Foundation, Inc. Multiphoton-lasermikroskopie
US5812308A (en) * 1995-12-20 1998-09-22 Spectra Physics Lasers, Inc. Mode locked laser and amplifier
US5814820A (en) * 1996-02-09 1998-09-29 The Board Of Trustees Of The University Of Illinois Pump probe cross correlation fluorescence frequency domain microscope and microscopy
US6545240B2 (en) * 1996-02-16 2003-04-08 Huron Valley Steel Corporation Metal scrap sorting system
US5761111A (en) * 1996-03-15 1998-06-02 President And Fellows Of Harvard College Method and apparatus providing 2-D/3-D optical information storage and retrieval in transparent materials
US5981956A (en) * 1996-05-16 1999-11-09 Affymetrix, Inc. Systems and methods for detection of labeled materials
DE19622359B4 (de) * 1996-06-04 2007-11-22 Carl Zeiss Jena Gmbh Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
DE19744302B4 (de) * 1996-06-04 2008-04-17 Carl Zeiss Jena Gmbh Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
US5754291A (en) * 1996-09-19 1998-05-19 Molecular Dynamics, Inc. Micro-imaging system
US6745067B1 (en) * 1998-09-14 2004-06-01 Lucid, Inc. System for marking the locations of imaged tissue with respect to the surface of the tissue
US6525862B2 (en) 1996-10-30 2003-02-25 Photogen, Inc. Methods and apparatus for optical imaging
US5832931A (en) * 1996-10-30 1998-11-10 Photogen, Inc. Method for improved selectivity in photo-activation and detection of molecular diagnostic agents
US20060095097A1 (en) * 1996-10-30 2006-05-04 Provectus Devicetech, Inc. Treatment of pigmented tissue using optical energy
US7353829B1 (en) * 1996-10-30 2008-04-08 Provectus Devicetech, Inc. Methods and apparatus for multi-photon photo-activation of therapeutic agents
US5829448A (en) * 1996-10-30 1998-11-03 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US7036516B1 (en) 1996-10-30 2006-05-02 Xantech Pharmaceuticals, Inc. Treatment of pigmented tissues using optical energy
US6267913B1 (en) 1996-11-12 2001-07-31 California Institute Of Technology Two-photon or higher-order absorbing optical materials and methods of use
US6608228B1 (en) * 1997-11-07 2003-08-19 California Institute Of Technology Two-photon or higher-order absorbing optical materials for generation of reactive species
WO1998021521A1 (en) * 1996-11-12 1998-05-22 California Institute Of Technology Two-photon or higher-order absorbing optical materials and methods of use
JP3917731B2 (ja) * 1996-11-21 2007-05-23 オリンパス株式会社 レーザ走査顕微鏡
US6148114A (en) * 1996-11-27 2000-11-14 Ultrapointe Corporation Ring dilation and erosion techniques for digital image processing
DE19653413C2 (de) * 1996-12-22 2002-02-07 Stefan Hell Rastermikroskop, bei dem eine Probe in mehreren Probenpunkten gleichzeitig optisch angeregt wird
DE19723873B4 (de) * 1997-06-06 2004-02-05 Evotec Oai Ag Verfahren und Vorrichtung zur Bewegungserfassung eines sich zumindest zeitweilig periodisch bewegenden Objekts
US6826422B1 (en) 1997-01-13 2004-11-30 Medispectra, Inc. Spectral volume microprobe arrays
US6847490B1 (en) 1997-01-13 2005-01-25 Medispectra, Inc. Optical probe accessory device for use in vivo diagnostic procedures
CA2279574C (en) 1997-01-31 2007-07-24 The Horticulture & Food Research Institute Of New Zealand Ltd. Optical apparatus
US5836877A (en) 1997-02-24 1998-11-17 Lucid Inc System for facilitating pathological examination of a lesion in tissue
US5762607A (en) * 1997-03-19 1998-06-09 Schotland; John Carl Emission tomography system and method using direct reconstruction of scattered radiation
AU6569198A (en) * 1997-03-19 1998-10-12 Lucid Technologies, Inc. Cellular surgery utilizing confocal microscopy
US6208886B1 (en) * 1997-04-04 2001-03-27 The Research Foundation Of City College Of New York Non-linear optical tomography of turbid media
US5995281A (en) * 1997-04-09 1999-11-30 Carl Zeiss Jena Gmbh Device for coupling the radiation of short-pulse lasers in an optical beam path of a microscope
US6316950B1 (en) * 1997-05-15 2001-11-13 Lucent Technologies Inc. Method and apparatus for imaging semiconductor devices
RO114383B1 (ro) * 1997-05-21 2000-02-28 Storex Technologies S.R.L. Dispozitiv pentru înscrierea tridimensională a informaţiei digitale într-o memorie optică de tip worm
US6096496A (en) * 1997-06-19 2000-08-01 Frankel; Robert D. Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis
US6020591A (en) * 1997-07-11 2000-02-01 Imra America, Inc. Two-photon microscopy with plane wave illumination
US6469311B1 (en) 1997-07-16 2002-10-22 Molecular Devices Corporation Detection device for light transmitted from a sensed volume
US6071748A (en) 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
DE19733194B4 (de) * 1997-08-01 2005-06-16 Carl Zeiss Jena Gmbh Laser-Scanning-Mikroskop
DE19733195B4 (de) * 1997-08-01 2006-04-06 Carl Zeiss Jena Gmbh Hoch-Kompaktes Laser Scanning Mikroskop mit integriertem Kurzpuls Laser
US6771417B1 (en) * 1997-08-01 2004-08-03 Carl Zeiss Jena Gmbh Applications of adaptive optics in microscopy
DE19733193B4 (de) * 1997-08-01 2005-09-08 Carl Zeiss Jena Gmbh Mikroskop mit adaptiver Optik
US6466040B1 (en) * 1997-08-01 2002-10-15 Carl Zeiss Jena Gmbh Three dimensional optical beam induced current (3-D-OBIC)
US5978695A (en) * 1997-08-18 1999-11-02 Lucid Inc. System for imaging mechanically stabilized tissue
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6992761B2 (en) 1997-09-20 2006-01-31 Molecular Devices Corporation Broad range light detection system
US6326605B1 (en) 1998-02-20 2001-12-04 Ljl Biosystems, Inc. Broad range light detection system
WO2000006991A2 (en) 1998-07-27 2000-02-10 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
WO2000006990A2 (en) 1998-07-27 2000-02-10 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
US6576476B1 (en) 1998-09-02 2003-06-10 Ljl Biosystems, Inc. Chemiluminescence detection method and device
US6825921B1 (en) 1999-11-10 2004-11-30 Molecular Devices Corporation Multi-mode light detection system
US6121603A (en) * 1997-12-01 2000-09-19 Hang; Zhijiang Optical confocal device having a common light directing means
US8974363B2 (en) * 1997-12-11 2015-03-10 Provectus Pharmatech, Inc. Topical medicaments and methods for photodynamic treatment of disease
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6132643A (en) * 1998-01-06 2000-10-17 Pavel; Eugen Fluorescent photosensitive vitroceramics and process for the production thereof
DE19801139B4 (de) * 1998-01-14 2016-05-12 Till Photonics Gmbh Punktabtastendes Luminiszenz-Mikroskop
WO1999037999A1 (en) 1998-01-27 1999-07-29 Wisconsin Alumni Research Foundation Signal enhancement for fluorescence microscopy
US6413252B1 (en) 1998-02-26 2002-07-02 Lucid, Inc. Confocal microscope for facilitating cryosurgery of tissue
US6855941B1 (en) * 1998-03-11 2005-02-15 Olympus Optical Co., Ltd. Laser microscope
US20030036855A1 (en) 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
KR100618502B1 (ko) * 1998-03-16 2006-09-01 지이 헬스케어 바이오-사이언시즈 코프. 공초점 마이크로스코피 영상 시스템에서 사용하기 위한 전자기 방출의 집속 시스템 및 방법
US5936728A (en) * 1998-04-14 1999-08-10 Noran Instruments, Inc. Flash photolysis method and apparatus
WO1999054784A1 (en) 1998-04-21 1999-10-28 University Of Connecticut Free-form nanofabrication using multi-photon excitation
US5880006A (en) 1998-05-22 1999-03-09 Vlsi Technology, Inc. Method for fabrication of a semiconductor device
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
DE19829944C2 (de) * 1998-07-04 2002-03-28 Zeiss Carl Jena Gmbh Verfahren und Anordnung zur Gerätekonfiguration eines Fluoreszenz-Laserscanmikroskops
US6228787B1 (en) 1998-07-27 2001-05-08 Eugen Pavel Fluorescent photosensitive glasses and process for the production thereof
ES2296398T3 (es) 1998-07-30 2008-04-16 Xy, Inc. Sistema de inseminacion equina artificial no quirurgica.
US8557298B2 (en) * 1998-08-06 2013-10-15 Provectus Pharmatech, Inc. Medicaments for chemotherapeutic treatment of disease
KR20010072307A (ko) * 1998-08-06 2001-07-31 스무크 존 질병의 표적화 국소치료를 위한 개선된 방법
US20090117199A1 (en) * 1998-08-06 2009-05-07 Scott Timothy C Method of treatment of cancer
EP1121052B1 (de) 1998-09-14 2018-07-25 Lucid, Inc. Bildgebung von chirurgischen biopsien
US7227630B1 (en) 1998-09-14 2007-06-05 Lucid, Inc. Imaging of surgical biopsies
DE19851240C1 (de) * 1998-11-06 2000-03-02 Europ Lab Molekularbiolog Fluoreszenzmikroskop mit Mehrphotonenanregung
US9192780B2 (en) 1998-11-30 2015-11-24 L'oreal Low intensity light therapy for treatment of retinal, macular, and visual pathway disorders
US20060212025A1 (en) * 1998-11-30 2006-09-21 Light Bioscience, Llc Method and apparatus for acne treatment
US6283956B1 (en) * 1998-11-30 2001-09-04 David H. McDaniels Reduction, elimination, or stimulation of hair growth
US6936044B2 (en) * 1998-11-30 2005-08-30 Light Bioscience, Llc Method and apparatus for the stimulation of hair growth
US6887260B1 (en) * 1998-11-30 2005-05-03 Light Bioscience, Llc Method and apparatus for acne treatment
WO2000037917A2 (en) 1998-12-23 2000-06-29 Medispectra, Inc. Systems and methods for optical examination of samples
JP2002532181A (ja) 1998-12-23 2002-10-02 メディスペクトラ， インコーポレイテッド 頚部のスクリーニングのための光学的方法およびシステム
JP3099063B2 (ja) * 1998-12-28 2000-10-16 大阪大学長 多光子顕微鏡
DE19901381A1 (de) * 1999-01-15 2000-07-20 Joerg Enderlein Verfahren und Vorrichtung zur optischen Detektion eines Partikels
IL128519A0 (en) * 1999-02-14 2000-06-01 Aaron Lewis Deconvolving far-field optical images beyond the diffraction limit by using scanned-probe optical and non-optical data as the constraint in mathematical constraint algorithms
WO2000049447A1 (en) * 1999-02-17 2000-08-24 Lucid, Inc. Tissue specimen holder
WO2000049392A1 (en) 1999-02-17 2000-08-24 Lucid, Inc. Cassette for facilitating optical sectioning of a retained tissue specimen
AU3005400A (en) 1999-02-23 2000-09-14 Ljl Biosystems, Inc. Frequency-domain light detection device
DE19908883A1 (de) 1999-03-02 2000-09-07 Rainer Heintzmann Verfahren zur Erhöhung der Auflösung optischer Abbildung
DE19919091C2 (de) * 1999-04-27 2002-01-17 Zeiss Carl Jena Gmbh Anordnung zur Einstellung der Laserleistung und/oder der Pulslänge eines Kurzpulslasers in einem Mikroskop
DE69906253D1 (de) * 1999-04-29 2003-04-30 Univ Erasmus Bestimmung von Analytbeweglichkeit
US6449039B1 (en) 1999-07-28 2002-09-10 Thermo Noran Inc. Laser scanning fluorescence microscopy with compensation for spatial dispersion of fast laser pulses
DE19935766A1 (de) * 1999-07-29 2001-02-01 Friedrich Schiller Uni Jena Bu Verfahren zur optischen Anregung von Fluorophor-markierter DNA und RNA
EP1203218A1 (de) * 1999-07-30 2002-05-08 California Institute of Technology System und verfahren zur überwachung von zellulären aktivität
US6445939B1 (en) 1999-08-09 2002-09-03 Lightlab Imaging, Llc Ultra-small optical probes, imaging optics, and methods for using same
DE19939706C2 (de) * 1999-08-18 2002-09-05 Forschungsverbund Berlin Ev Fluorophor für die Multi-Photonen-Laser-Scanning-Mikroskopie
JP4680337B2 (ja) * 1999-09-20 2011-05-11 オリンパス株式会社 走査型レーザ顕微鏡
US7024316B1 (en) * 1999-10-21 2006-04-04 Dakocytomation Colorado, Inc. Transiently dynamic flow cytometer analysis system
US7167615B1 (en) 1999-11-05 2007-01-23 Board Of Regents, The University Of Texas System Resonant waveguide-grating filters and sensors and methods for making and using same
AU1476801A (en) * 1999-11-10 2001-06-06 Lucid, Inc. System for optically sectioning and mapping surgically excised tissue
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
DE19956620A1 (de) * 1999-11-25 2001-05-31 Zeiss Carl Jena Gmbh Verfahren zur Erfassung von Fluoreszenzerscheinungen in einem Mikroskop
US6902935B2 (en) * 1999-12-15 2005-06-07 Medispectra, Inc. Methods of monitoring effects of chemical agents on a sample
US7187810B2 (en) 1999-12-15 2007-03-06 Medispectra, Inc. Methods and systems for correcting image misalignment
US7260248B2 (en) 1999-12-15 2007-08-21 Medispectra, Inc. Image processing using measures of similarity
AU779659B2 (en) 2000-02-08 2005-02-03 Cornell Research Foundation Inc. Multiphoton excitation through optical fibers for fluorescence spectroscopy
WO2001067176A1 (en) 2000-03-09 2001-09-13 Xerox Corporation Three dimensional optical memory storage
EP1272829A1 (de) * 2000-04-14 2003-01-08 Zeptosens AG Gitter-wellenleiter-struktur zur verstärkung eines anregungsfeldes und deren verwendung
US20020001089A1 (en) * 2000-04-18 2002-01-03 Price Jeffrey H. Multiparallel three dimensional optical microscopy system
EP2258169B1 (de) 2000-05-09 2019-07-10 Xy, Llc Verfahren zur Trennung von X-Chromosom-Lager und Y-Chromosom-Lagerpopulationen von Spermatozoiden
DE10027726A1 (de) * 2000-06-03 2001-12-06 Bundesdruckerei Gmbh Sensor für die Echtheitserkennung von Signets auf Dokumenten
DE10027323B4 (de) * 2000-06-05 2013-09-26 Leica Microsystems Cms Gmbh Verfahren zum Generieren eines dreidimensionalen Objekts
JP2004503928A (ja) * 2000-06-15 2004-02-05 スリーエム　イノベイティブ　プロパティズ　カンパニー 多方向光反応吸収方法
AU2001266919A1 (en) * 2000-06-15 2001-12-24 3M Innovative Properties Company Multipass multiphoton absorption method and apparatus
US6852766B1 (en) * 2000-06-15 2005-02-08 3M Innovative Properties Company Multiphoton photosensitization system
US7026103B2 (en) * 2000-06-15 2006-04-11 3M Innovative Properties Company Multicolor imaging using multiphoton photochemical processes
JP4786858B2 (ja) * 2000-06-15 2011-10-05 スリーエム イノベイティブ プロパティズ カンパニー 封入光学素子を提供するための多光子硬化
KR100810546B1 (ko) * 2000-06-15 2008-03-18 쓰리엠 이노베이티브 프로퍼티즈 캄파니 삼차원 광학 소자의 가공 방법
WO2001096958A2 (en) * 2000-06-15 2001-12-20 3M Innovative Properties Company Process for producing microfluidic articles
EP1164401B1 (de) 2000-06-17 2005-03-09 Leica Microsystems Heidelberg GmbH Verschränkte-Photonen-Mikroskop
DE10115590B4 (de) * 2000-06-17 2020-11-05 Leica Microsystems Cms Gmbh Scanmikroskop
US6898367B2 (en) * 2000-06-17 2005-05-24 Leica Microsystems Heidelberg Gmbh Method and instrument for microscopy
DE20122783U1 (de) * 2000-06-17 2007-11-15 Leica Microsystems Cms Gmbh Anordnung zum Untersuchen mikroskopischer Präparate mit einem Scanmikroskop und Beleuchtungseinrichtung für ein Scanmikroskop
US6687000B1 (en) 2000-06-26 2004-02-03 Wisconsin Alumni Research Foundation Photon-sorting spectroscopic microscope system
DE10035190C5 (de) * 2000-07-20 2009-07-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Vorrichtung zur Fluoreszenzmessung
US20070196815A1 (en) * 2000-08-02 2007-08-23 Jason Lappe Positive Selection Procedure for Optically Directed Selection of Cells
DE10039520A1 (de) 2000-08-08 2002-02-21 Leica Microsystems Vorrichtung zur Untersuchung und Manipulation von mikroskopischen Objekten
DE10044308A1 (de) 2000-09-07 2002-03-21 Leica Microsystems Verfahren und Vorrichtung zur Detektion von Fluoreszenzlicht bei der konfokalen Rastermikroskopie
US7321394B1 (en) 2000-09-29 2008-01-22 Lucid, Inc. Automatic gain control for a confocal imaging system
JP4693972B2 (ja) 2000-09-29 2011-06-01 オリンパス株式会社 レーザ顕微鏡
US6295123B1 (en) 2000-10-13 2001-09-25 Taiwan Semiconductor Manufacturing Company Multiple photon absorption for high resolution lithography
US7003345B1 (en) 2000-10-17 2006-02-21 Lucid, Inc. System and method for enhancing microscope images of tissue using citric acid and agents of the like
US6369928B1 (en) 2000-11-01 2002-04-09 Optical Biopsy Technologies, Inc. Fiber-coupled, angled-dual-illumination-axis confocal scanning microscopes for performing reflective and two-photon fluorescence imaging
DE60128138T2 (de) * 2000-11-02 2008-01-03 Cornell Research Foundation, Inc. In vivo multiphoton diagnostische detektion und bilddarstellung einer neurodegenerativen erkrankung
US7194118B1 (en) 2000-11-10 2007-03-20 Lucid, Inc. System for optically sectioning and mapping surgically excised tissue
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
CA2468772C (en) 2000-11-29 2013-10-29 George E. Seidel System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US6414779B1 (en) 2000-11-30 2002-07-02 Opeical Biopsy Technologies, Inc. Integrated angled-dual-axis confocal scanning endoscopes
WO2002048693A1 (fr) * 2000-12-14 2002-06-20 Olympus Optical Co., Ltd. Analyseur fluorometrique et analyse fluorometrique
US6839661B2 (en) 2000-12-15 2005-01-04 Medispectra, Inc. System for normalizing spectra
AU2002245119A1 (en) 2000-12-15 2002-07-30 Sloan-Kettering Institute For Cancer Research Beam-steering of multi-chromatic light using acousto-optical deflectors and dispersion-compensatory optics
CA2328684A1 (en) * 2000-12-15 2002-06-15 Yahia Gawad Photon-triggered luminescent assay
US8208505B2 (en) * 2001-01-30 2012-06-26 Board Of Trustees Of Michigan State University Laser system employing harmonic generation
US7567596B2 (en) 2001-01-30 2009-07-28 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
US7583710B2 (en) * 2001-01-30 2009-09-01 Board Of Trustees Operating Michigan State University Laser and environmental monitoring system
US7450618B2 (en) * 2001-01-30 2008-11-11 Board Of Trustees Operating Michigan State University Laser system using ultrashort laser pulses
US7609731B2 (en) * 2001-01-30 2009-10-27 Board Of Trustees Operating Michigan State University Laser system using ultra-short laser pulses
WO2002061799A2 (en) * 2001-01-30 2002-08-08 Board Of Trustees Operating Michigan State University Control system and apparatus for use with laser excitation or ionization
US7973936B2 (en) * 2001-01-30 2011-07-05 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
EP1372552B1 (de) * 2001-03-27 2017-03-01 WaveLight GmbH Vorrichtung zur bearbeitung und diagnose von augengewebe
CA2443317C (en) 2001-03-30 2013-06-18 The Arizona Board Of Regents On Behalf Of The University Of Arizona Materials, methods, and uses for photochemical generation of acids and/or radical species
US20040052489A1 (en) * 2001-04-02 2004-03-18 Duveneck Gert Ludwig Optical structure for multi-photon excitation and the use thereof
JP3885511B2 (ja) * 2001-04-11 2007-02-21 ソニー株式会社 レーザー光発生装置及び方法
DE10120425C2 (de) * 2001-04-26 2003-12-18 Leica Microsystems Scanmikroskop
US20030211009A1 (en) * 2001-05-18 2003-11-13 Buchanan Kris S. Rapid multi-material sample input system
US20040012872A1 (en) * 2001-06-14 2004-01-22 Fleming Patrick R Multiphoton absorption method using patterned light
US6998214B2 (en) * 2001-07-13 2006-02-14 The Trustees Of Boston College Methods for three-dimensional optical data storage and retrieval
US7336988B2 (en) * 2001-08-08 2008-02-26 Lucent Technologies Inc. Multi-photon endoscopy
US6643071B2 (en) 2001-12-21 2003-11-04 Lucent Technologies Inc. Graded-index lens microscopes
DE10206980A1 (de) * 2002-02-20 2003-08-21 Leica Microsystems Mikroskop, Detektor und Verfahren zur Mikroskopie
US20040133112A1 (en) * 2002-03-08 2004-07-08 Milind Rajadhyaksha System and method for macroscopic and confocal imaging of tissue
WO2003093795A2 (en) * 2002-05-03 2003-11-13 Immunivest Corporation Device and method for analytical cell imaging
JP4175833B2 (ja) * 2002-05-23 2008-11-05 オリンパス株式会社 レーザ顕微鏡
DE10228374A1 (de) * 2002-06-25 2004-01-15 Leica Microsystems Heidelberg Gmbh Verfahren zur Mikroskopie und Mikroskop
US7459696B2 (en) 2003-04-18 2008-12-02 Schomacker Kevin T Methods and apparatus for calibrating spectral data
US7136518B2 (en) 2003-04-18 2006-11-14 Medispectra, Inc. Methods and apparatus for displaying diagnostic data
US7309867B2 (en) 2003-04-18 2007-12-18 Medispectra, Inc. Methods and apparatus for characterization of tissue samples
US6933154B2 (en) 2002-07-09 2005-08-23 Medispectra, Inc. Optimal windows for obtaining optical data for characterization of tissue samples
US6818903B2 (en) * 2002-07-09 2004-11-16 Medispectra, Inc. Method and apparatus for identifying spectral artifacts
US7282723B2 (en) 2002-07-09 2007-10-16 Medispectra, Inc. Methods and apparatus for processing spectral data for use in tissue characterization
US7469160B2 (en) 2003-04-18 2008-12-23 Banks Perry S Methods and apparatus for evaluating image focus
US6768918B2 (en) 2002-07-10 2004-07-27 Medispectra, Inc. Fluorescent fiberoptic probe for tissue health discrimination and method of use thereof
US7103401B2 (en) 2002-07-10 2006-09-05 Medispectra, Inc. Colonic polyp discrimination by tissue fluorescence and fiberoptic probe
NZ538462A (en) 2002-08-01 2008-06-30 Xy Inc Low pressure sperm cell separation system
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
US7855078B2 (en) 2002-08-15 2010-12-21 Xy, Llc High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
US8227256B2 (en) * 2002-09-27 2012-07-24 Saloma Caesar A Two-color (two-photon) excitation with focused excitation beams and a raman shifter
DE10250012B4 (de) * 2002-10-25 2005-06-23 Universität Kassel Verfahren zur Bestimmung der Oberflächenstruktur einer Materialprobe mit ultrakurzen Laserpulsen und Vorrichtung zur Durchführung des Verfahrens
DE10250568A1 (de) * 2002-10-28 2004-05-13 Carl Zeiss Jena Gmbh Verfahren zur Verbesserung der Tiefendiskriminierung optisch abbildender Systeme
WO2004052181A2 (en) * 2002-12-11 2004-06-24 The Regents Of The University Of California Device and method for inducing vascular injury and/or blockage in an animal model
US7141801B2 (en) * 2002-12-26 2006-11-28 Applied Precision, Llc System and method of illuminating living cells for imaging
DE10300157B4 (de) * 2003-01-07 2016-08-25 Leica Microsystems Cms Gmbh Konfokales 4-Pi-Mikroskop und Verfahren zur konfokalen 4-Pi-Mikroskopie
US7130042B2 (en) * 2003-03-06 2006-10-31 Board Of Trustees Of The Leland Stanford Junior University Dual axis fluorescence microscope with modulated input
EP2308416B1 (de) 2003-03-28 2015-01-07 Inguran, LLC Vorrichtung und Verfahren zur Bereitstellung von geschlechtssortierten Tierspermien
DE10314750A1 (de) * 2003-03-31 2004-11-04 Leica Microsystems Heidelberg Gmbh Rastermikroskop zur Detektion eines Objekts
KR20060041161A (ko) 2003-04-10 2006-05-11 라이트 바이오사이언스, 엘엘씨 세포 증식 및 유전자 발현의 조절을 위한 광조절 방법 및광조절 장치
US7151270B2 (en) * 2003-05-02 2006-12-19 Leica Microsystems Cms Gmbh Method for classifying object image regions of an object to be detected using a scanning microscope
AU2004242121B2 (en) 2003-05-15 2010-06-24 Xy, Llc. Efficient haploid cell sorting for flow cytometer systems
US7091500B2 (en) 2003-06-20 2006-08-15 Lucent Technologies Inc. Multi-photon endoscopic imaging system
US7545494B2 (en) * 2003-07-23 2009-06-09 Bayer Technology Services Gmbh Analytical system and method for analyzing nonlinear optical signals
CA2533129A1 (en) * 2003-07-31 2005-02-10 Light Bioscience, Llc System and method for the photodynamic treatment of burns, wounds, and related skin disorders
AU2004297876A1 (en) * 2003-08-19 2005-06-23 Cornell Research Foundation, Inc. Optical fiber delivery and collection system for biological applications such as multiphoton microscopy, spectroscopy, and endoscopy
US20050056193A1 (en) * 2003-09-11 2005-03-17 Chep International, Inc Pallet
US20050142608A1 (en) * 2003-09-22 2005-06-30 Yokogawa Electric Corporation Screening method and device, and new drug screening method and device
DE10351414A1 (de) * 2003-10-30 2005-06-23 Carl Zeiss Jena Gmbh Laser-Scanning-Mikroskop mit einem non-descannten Detektions- und/oder Beobachtungsstrahlengang
US7706863B2 (en) 2004-01-21 2010-04-27 University Of Washington Methods for assessing a physiological state of a mammalian retina
JP2005275199A (ja) * 2004-03-26 2005-10-06 Yokogawa Electric Corp ３次元共焦点顕微鏡システム
US7892725B2 (en) 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
EP1582858A1 (de) * 2004-03-29 2005-10-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Anregung der Moleküle von einem ersten Zustand in einen zweiten Zustand mit einem optischen Signal
US7170675B2 (en) * 2004-05-19 2007-01-30 Celloptic, Inc. Method and system for wide-field multi-photon microscopy having a confocal excitation plane
US7355702B2 (en) * 2004-06-21 2008-04-08 Olympus Corporation Confocal observation system
TWI261605B (en) * 2004-06-30 2006-09-11 Ind Tech Res Inst Dye compositon of the optical recording medium
AR049732A1 (es) 2004-07-22 2006-08-30 Monsanto Co Proceso para enriquecer una poblacion de celulas de esperma
WO2006015056A2 (en) * 2004-07-27 2006-02-09 Dakocytomation Denmarks A/S Enhancing flow cytometry discrimination with geometric transformation
WO2006078857A2 (en) * 2005-01-16 2006-07-27 Baer Stephen C Single wavelength stimulated emission depletion microscopy
HU227859B1 (en) * 2005-01-27 2012-05-02 E Szilveszter Vizi Real-time 3d nonlinear microscope measuring system and its application
WO2006088841A1 (en) * 2005-02-14 2006-08-24 Board Of Trustees Of Michigan State University Ultra-fast laser system
US7897638B2 (en) * 2005-04-12 2011-03-01 Philadelphia Health & Education Corporation Synthesis of nitrodibenzylfuran chromophore for photodeprotection of organic molecules
WO2006128442A1 (de) * 2005-05-31 2006-12-07 W.O.M. World Of Medicine Ag Verfahren und vorrichtung zur optischen charakterisierung von gewebe
JP4621893B2 (ja) * 2005-08-22 2011-01-26 独立行政法人産業技術総合研究所 物体の調査方法及び調査装置
EP1957959A2 (de) 2005-11-30 2008-08-20 Board of Trustees of Michigan State University Identifizierung molekularer eigenschaften auf laserbasis
US7864996B2 (en) * 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
US7525724B2 (en) * 2006-03-16 2009-04-28 The University Of Kansas Laser system for photonic excitation investigation
JP4759425B2 (ja) 2006-03-28 2011-08-31 オリンパス株式会社 多光子励起型観察装置
US9018562B2 (en) 2006-04-10 2015-04-28 Board Of Trustees Of Michigan State University Laser material processing system
US7773300B2 (en) * 2006-05-12 2010-08-10 Semrock, Inc. Multiphoton fluorescence filters
DE102006029809B3 (de) * 2006-06-28 2007-11-08 Ltb Lasertechnik Berlin Gmbh Ortsaufgelöstes Messverfahren für die Detektion von Melanin in Fluorophorgemischen in einer Festkörperprobe
US8194247B2 (en) * 2006-06-29 2012-06-05 Agency For Science, Technology And Research SHG quantification of matrix-related tissue dynamic and disease
DE102006034910B4 (de) 2006-07-28 2019-05-02 Carl Zeiss Microscopy Gmbh Mikroskop umfassend einen Strahlvereiniger
DE102006034914A1 (de) * 2006-07-28 2008-01-31 Carl Zeiss Microimaging Gmbh Verfahren und Anordnung zur Ansteuerung eines Mikroskops, insbesondere eines Laser-Scanning-Mikroskopes
DE102006034906A1 (de) 2006-07-28 2008-01-31 Carl Zeiss Microimaging Gmbh Laser-Scanning-Mikroskop und Verfahren zu seinem Betrieb
WO2008019130A2 (en) * 2006-08-04 2008-02-14 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health Wide-area fluorescence detection system for multi-photon microscopy
GB0617945D0 (en) 2006-09-12 2006-10-18 Ucl Business Plc Imaging apparatus and methods
DE102006046925A1 (de) * 2006-09-28 2008-04-03 Jenlab Gmbh Verfahren und Anordnung zur Laser-Endoskopie für die Mikrobearbeitung
US7973927B2 (en) * 2006-09-29 2011-07-05 Uwm Research Foundation, Inc. Two-photon microscope with spectral resolution
US7480045B2 (en) * 2006-10-31 2009-01-20 Academia Sinica Controlling pulses in optical microscopy
US20080116392A1 (en) * 2006-11-20 2008-05-22 Celloptic, Inc. Method and system for wide-field multi-photon microscopy having a confocal excitation plane
US7936503B2 (en) * 2007-02-19 2011-05-03 Olympus Corporation Laser scanning microscope
US7867778B2 (en) * 2007-02-23 2011-01-11 Visiongate, Inc. Fluid focusing for positional control of a specimen for 3-D imaging
CN101254091B (zh) * 2007-02-28 2010-08-18 深圳大学 一种视网膜成像的方法
DE102007021378A1 (de) * 2007-05-04 2008-11-06 Ape Angewandte Physik Und Elektronik Gmbh Verfahren und optische Anordnung zum Erzeugen eines nicht-linearen optischen Signals an einem durch ein Anregungsfeld angeregten Material sowie Verwendung des Verfahrens und der optischen Anordnung
US8958156B1 (en) 2007-05-30 2015-02-17 Semrock, Inc. Interference filter for non-zero angle of incidence spectroscopy
DE102007025821A1 (de) 2007-06-02 2008-12-04 Carl Zeiss Microimaging Gmbh Anordnung und Verfahren zur zeitlichen Einstellung der Pulse eines Kurzpulslasers
US20110189702A1 (en) * 2007-07-11 2011-08-04 Ya-Ping Sun Photoluminescent materials for multiphoton imaging
WO2009016806A1 (ja) * 2007-07-27 2009-02-05 Nikon Corporation マルチフォトンレーザ走査顕微鏡装置
DE102007039111B4 (de) 2007-08-18 2014-11-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. STED-Fluoreszenzmikroskopie mit Zweiphotonen-Anregung
US8663604B2 (en) * 2007-09-06 2014-03-04 The Regents Of The University Of California Method for measuring neurotransmitters in vivo
US7961764B2 (en) * 2007-09-12 2011-06-14 Howard Hughes Medical Institute Nonlinear imaging using passive pulse splitters and related technologies
US9354370B1 (en) 2007-09-25 2016-05-31 Semrock, Inc. Optical thin-film notch filter with very wide pass band regions
US7767441B2 (en) 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7811810B2 (en) * 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US8921826B2 (en) * 2007-11-27 2014-12-30 Technion Research & Development Foundation Limited Light source based on simultaneous two-photon emission
WO2009086122A2 (en) 2007-12-21 2009-07-09 Board Of Trustees Of Michigan State University Control in ultrashort laser systems by a deformable mirror in the stretcher
JP5202971B2 (ja) * 2008-01-28 2013-06-05 オリンパス株式会社 測定装置及び測定方法
US8143600B2 (en) 2008-02-18 2012-03-27 Visiongate, Inc. 3D imaging of live cells with ultraviolet radiation
US8090183B2 (en) * 2009-03-12 2012-01-03 Visiongate, Inc. Pattern noise correction for pseudo projections
FR2930031A1 (fr) * 2008-04-14 2009-10-16 Centre Nat Rech Scient Dispositif et procede d'analyse exaltee d'un echantillon de particules.
US8675699B2 (en) * 2009-01-23 2014-03-18 Board Of Trustees Of Michigan State University Laser pulse synthesis system
JP5605992B2 (ja) * 2009-01-30 2014-10-15 株式会社東芝 顕微鏡および観測方法
FR2941787B1 (fr) 2009-02-04 2011-04-15 Ecole Polytech Procede et dispositif d'acquisition de signaux en microscopie laser a balayage.
US8254023B2 (en) * 2009-02-23 2012-08-28 Visiongate, Inc. Optical tomography system with high-speed scanner
WO2010141128A2 (en) 2009-03-05 2010-12-09 Board Of Trustees Of Michigan State University Laser amplification system
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US8432546B2 (en) 2009-03-18 2013-04-30 Robert D Frankel Method and system for stimulated Raman microscopy beyond the diffraction limit
US8879150B1 (en) 2009-03-20 2014-11-04 Semrock, Inc. Optical thin-film polarizing bandpass filter
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
US8759792B2 (en) * 2009-07-10 2014-06-24 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Non-contact total emission detection method and system for multi-photon microscopy
JP5852305B2 (ja) 2009-10-28 2016-02-03 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 分解能の向上した顕微鏡法および顕微鏡
US8441633B2 (en) 2009-10-29 2013-05-14 California Institute Of Technology Multiple-photon excitation light sheet illumination microscope
US20110147615A1 (en) * 2009-12-23 2011-06-23 Kintz Gregory J Method and apparatus for microscopic imaging system with wide field of view and high collection efficiency
US8441710B2 (en) * 2010-01-08 2013-05-14 Semrock, Inc. Tunable thin-film filter
US8630322B2 (en) 2010-03-01 2014-01-14 Board Of Trustees Of Michigan State University Laser system for output manipulation
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
DE102010013829A1 (de) 2010-03-26 2011-09-29 Carl Zeiss Microlmaging Gmbh Mikroskop und Verfahren zur Erfassung von Probenlicht
GB201006679D0 (en) 2010-04-21 2010-06-09 Ucl Business Plc Methods and apparatus to control acousto-optic deflectors
DE102010018967B4 (de) 2010-04-29 2021-11-04 Carl Zeiss Microscopy Gmbh Anordnungen und Verfahren zur nichtlinearen Mikroskopie
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
JP2012008261A (ja) * 2010-06-23 2012-01-12 Hamamatsu Photonics Kk 画像生成装置
US8575570B2 (en) 2010-08-25 2013-11-05 California Institute Of Technology Simultaneous orthogonal light sheet microscopy and computed optical tomography
US8432543B2 (en) * 2010-09-20 2013-04-30 Robert D Frankel Method and system for raman, fluorescence, lithographic, stimulated emission and photochemical imaging beyond the diffraction limit
DE102011013613A1 (de) 2010-10-01 2012-04-05 Carl Zeiss Microimaging Gmbh Mikroskop und Mikroskopierverfahren
DE102010047353A1 (de) 2010-10-01 2012-04-05 Carl Zeiss Microimaging Gmbh Laser-Scanning-Mikroskop mit umschaltbarer Betriebsweise
US9494781B2 (en) * 2011-01-19 2016-11-15 California Institute Of Technology Plane-projection multi-photon microscopy
US9103721B2 (en) 2011-04-07 2015-08-11 Uwm Research Foundation, Inc. High speed microscope with spectral resolution
WO2012135961A1 (en) * 2011-04-08 2012-10-11 British Columbia Cancer Agency Branch Apparatus and methods for multiphoton microscopy
GB201106787D0 (en) 2011-04-20 2011-06-01 Ucl Business Plc Methods and apparatus to control acousto-optic deflectors
DE102011115944B4 (de) 2011-10-08 2013-06-06 Jenlab Gmbh Flexibles nichtlineares Laserscanning-Mikroskop zur nicht-invasiven dreidimensionalen Detektion
DE102011122230B8 (de) 2011-12-23 2023-07-06 Menlo Systems Gmbh Optikanordnung und Verfahren zum Untersuchen oder Bearbeiten eines Objekts
AU2013222070B2 (en) 2012-02-26 2016-07-07 Caliber Imaging & Diagnostics, Inc. Tissue specimen stage for an optical sectioning microscope
US9109879B2 (en) 2012-02-29 2015-08-18 Corning Incorporated Systems for and methods of characterizing the thickness profile of laminated glass structures
DE102012010207B4 (de) 2012-05-15 2024-02-29 Carl Zeiss Microscopy Gmbh Mikroskop und Mikroskopieverfahren
US9709791B2 (en) 2012-08-15 2017-07-18 Lucid, Inc. Systems and methods for imaging tissue
DE102012016346B4 (de) 2012-08-16 2023-01-05 Carl Zeiss Microscopy Gmbh Laser-Scanning-Mikroskop
US9304237B1 (en) 2012-12-10 2016-04-05 Semrock, Inc. Tunable band-pass filter
US9267893B2 (en) 2013-10-01 2016-02-23 Wisconsin Alumni Research Foundation Triple sum frequency coherent multidimensional imaging
US10317347B2 (en) * 2013-11-01 2019-06-11 Kla-Tencor Corp. Determining information for defects on wafers
JP6307903B2 (ja) * 2014-02-04 2018-04-11 株式会社Ihi 物質特定方法および物質特定システム
WO2015130651A1 (en) 2014-02-28 2015-09-03 Imra America, Inc. Multi-wavelength, ultrashort pulse generation and delivery, with applications in microscopy
AU2015256153B2 (en) 2014-05-05 2020-04-23 Caliber Imaging & Diagnostics, Inc. System and method for mapping the locations of captured confocal images of a lesion in skin tissue
TWI700473B (zh) 2014-06-04 2020-08-01 美商康寧公司 用於量測玻璃物品厚度的方法及系統
WO2016007954A1 (en) * 2014-07-11 2016-01-14 The University Of North Carolina At Chapel Hill Steering devices for two-photon excitation imaging systems
GB201414631D0 (en) * 2014-08-18 2014-10-01 Univ Singapore Apparatus and methods for simultaneous multimodal nonlinear optical microscopy for label-free bio-imaging
CN104198458B (zh) * 2014-09-26 2017-02-22 哈尔滨工业大学 一种飞秒激光双光子荧光生物显微成像系统及其成像方法
WO2016141339A1 (en) 2015-03-04 2016-09-09 Aramco Services Company Adaptive optics for imaging through highly scattering media in oil reservoir applications
US10514532B1 (en) 2015-09-27 2019-12-24 Caliber Imaging & Diagnostics, Inc. Confocal microscope having a positionable imaging head mounted on a boom stand
US11069054B2 (en) 2015-12-30 2021-07-20 Visiongate, Inc. System and method for automated detection and monitoring of dysplasia and administration of immunotherapy and chemotherapy
TWI619937B (zh) * 2016-01-15 2018-04-01 奇美視像科技股份有限公司 以多光子激發技術檢查物體之方法以及量測物體之裝置
FI20165148A (fi) 2016-02-25 2017-08-26 Arcdia Int Oy Ltd Kaksoisfotoniviritteistä fluoresenssia hyödyntävä bioaffiniteettimääritysmenetelmä
WO2017157950A1 (en) 2016-03-15 2017-09-21 Johann Wolfgang Goethe-Universität Light inducible antisense oligonucleotides for in vivo application
DE102016108745A1 (de) * 2016-05-11 2017-11-16 Hydro Aluminium Rolled Products Gmbh Verfahren und Vorrichtung für das legierungsabhängige Sortieren von Metallschrott, insbesondere Aluminiumschrott
US9785851B1 (en) 2016-06-30 2017-10-10 Huron Valley Steel Corporation Scrap sorting system
US10082657B2 (en) * 2016-08-17 2018-09-25 The United States Of America, As Represented By The Secretary Of The Navy Dual magnification apparatus and system for examining a single objective in a scanning optical microscope using two wavelengths of light
WO2018075562A1 (en) * 2016-10-19 2018-04-26 The Regents Of The University Of California Imaging platform based on nonlinear optical microscopy for rapid scanning large areas of tissue
ES2964060T3 (es) 2016-11-12 2024-04-03 Caliber Imaging & Diagnostics Inc Microscopio confocal con cabezal de formación de imágenes posicionable
CN110799831B (zh) * 2017-06-29 2022-05-31 学校法人东海大学 流体的测量方法、测量装置以及测量系统
RU2680664C1 (ru) * 2017-12-18 2019-02-25 Федеральное Государственное Бюджетное Образовательное Учреждение Высшего Образования "Казанский Национальный Исследовательский Технический Университет Им. А.Н. Туполева-Каи", Книту-Каи Способ регистрации быстрых флуоресцентных сигналов при помощи лазерного сканирующего конфокального микроскопа
WO2019148024A1 (en) 2018-01-26 2019-08-01 Park Jong Kang Systems and methods to reduce scattering in temporal focusing multiphoton microscopy
WO2019165480A1 (en) 2018-02-26 2019-08-29 Caliber Imaging & Diagnostics, Inc. System and method for macroscopic and microscopic imaging ex-vivo tissue
EP3542710A1 (de) 2018-03-23 2019-09-25 JenLab GmbH Multimodales bildgebungssystem und verfahren zur nicht-invasiven untersuchung eines untersuchungsobjekts
GB201804952D0 (en) 2018-03-27 2018-05-09 Pxyl Ltd Improved scanning optical microscope
US10823664B2 (en) 2018-06-22 2020-11-03 Wisconsin Alumni Research Foundation Ultrafast, multiphoton-pump, multiphoton-probe spectroscopy
US11808702B2 (en) * 2019-01-31 2023-11-07 The Rockefeller University Hybrid multi-photon microscopy
US11486818B2 (en) 2020-05-26 2022-11-01 Wisconsin Alumni Research Foundation Methods and systems for coherent multidimensional spectroscopy
CN113049561A (zh) * 2021-03-24 2021-06-29 雷振东 一种压缩光共聚焦探测装置及方法
CN116300310B (zh) * 2023-01-06 2024-04-16 之江实验室 一种利用光引发剂实现超分辨刻写与成像的方法和装置

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508208A (en) * 1967-12-27 1970-04-21 Bell Telephone Labor Inc Optical organic memory device
US4466080A (en) * 1975-01-27 1984-08-14 Formigraphic Engine Corporation Three-dimensional patterned media
US4288861A (en) * 1977-12-01 1981-09-08 Formigraphic Engine Corporation Three-dimensional systems
US4471470A (en) * 1977-12-01 1984-09-11 Formigraphic Engine Corporation Method and media for accessing data in three dimensions
DE3037983C2 (de) * 1980-10-08 1983-03-31 Fa. Carl Zeiss, 7920 Heidenheim Verfahren und Vorrichtung zur lichtinduzierten rastermikroskopischen Darstellung von Probenparametern in ihrer räumlichen Verteilung
US4405237A (en) * 1981-02-04 1983-09-20 The United States Of America As Represented By The Secretary Of The Navy Coherent anti-Stokes Raman device
SE455736B (sv) * 1984-03-15 1988-08-01 Sarastro Ab Forfaringssett och anordning for mikrofotometrering och efterfoljande bildsammanstellning
DE3422144A1 (de) * 1984-06-14 1985-12-19 Josef Prof. Dr. 6900 Heidelberg Bille Geraet zur darstellung flaechenhafter bereiche des menschlichen auges
DE3610165A1 (de) * 1985-03-27 1986-10-02 Olympus Optical Co., Ltd., Tokio/Tokyo Optisches abtastmikroskop
US4877965A (en) * 1985-07-01 1989-10-31 Diatron Corporation Fluorometer
DD254998A1 (de) * 1985-07-26 1988-03-16 Zeiss Jena Veb Carl Anordnung zur bildlichen darstellung und analyse von fluoreszenzsignalen
US4792341A (en) * 1986-06-19 1988-12-20 Clairol Incorporated Hair photobleaching
US4791310A (en) * 1986-10-02 1988-12-13 Syracuse University Fluorescence microscopy
JPS63131116A (ja) * 1986-11-21 1988-06-03 Hitachi Ltd 共焦点顕微鏡
NL8700612A (nl) * 1987-03-13 1988-10-03 Tno Confocale laserscanning microscoop.
US4827125A (en) * 1987-04-29 1989-05-02 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Confocal scanning laser microscope having no moving parts
US4887721A (en) * 1987-11-30 1989-12-19 The United States Of America As Represented By The United States Department Of Energy Laser particle sorter
JPH0315746A (ja) * 1988-05-13 1991-01-24 Hitachi Ltd 光検出型ｄｎａ検出方式

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007055530A1 (de) * 2007-11-21 2009-05-28 Carl Zeiss Ag Laserstrahlbearbeitung
US8389893B2 (en) 2007-11-21 2013-03-05 Nanoscribe Gmbh Laser beam machining

Also Published As

Publication number Publication date
ATE253727T1 (de) 2003-11-15
WO1991007651A1 (en) 1991-05-30
EP0500717A4 (en) 1992-12-09
US5034613A (en) 1991-07-23
JP2848952B2 (ja) 1999-01-20
EP0500717A1 (de) 1992-09-02
JPH05503149A (ja) 1993-05-27
ATE170636T1 (de) 1998-09-15
EP0807814A1 (de) 1997-11-19
DE69032621D1 (de) 1998-10-08
DE69034117D1 (de) 2003-12-11
ES2123501T5 (es) 2004-07-16
DK0807814T3 (da) 2004-03-15
DE69032621T3 (de) 2004-08-19
DE69034117T2 (de) 2004-08-19
HK1010411A1 (en) 1999-06-17
ES2123501T3 (es) 1999-01-16
EP0500717B1 (de) 1998-09-02
EP0500717B2 (de) 2003-11-19
EP0807814B1 (de) 2003-11-05
ES2210414T3 (es) 2004-07-01

Similar Documents

Publication Publication Date Title
DE69032621T2 (de) Zweiwellenlängenlaserabtastmikroskop
EP0052892B2 (de) Mit Laserstrahl arbeitende Abtastvorrichtung
ATE163762T1 (de) Photothermisches untersuchungsverfahren, einrichtung zu seiner durchführung und verwendung des verfahrens
CA2280154A1 (en) Multiple assays of cell specimens
ATE37625T1 (de) Geraet zur regelung des aufzeichnungsbuendels in einem optischen datenaufzeichnungsverfahren.
US6922279B2 (en) Harmonic generation microscopy
CN102656421A (zh) 利用结构化光学元件和聚焦光束的激光刻图
WO1997025723A3 (de) Röntgenmikroskop mit zonenplatten
DE69317957T2 (de) Vorrichtung zur Erzeugung von Laserstrahlen
DE69221457D1 (de) Einrichtung, in der Frequenzerhöhung von elektromagnetischer Strahlung auftritt, und eine solche Einrichtung enthaltendes Gerät zum optischen Abtasten einer Informationsebene
LT95051A (en) Method and apparatus for laser marking of products from transparent materials
GB2249843A (en) Image production
GB2360008A (en) Material processing applications of lasers using optical breakdown
ATE250208T1 (de) System und methode zur erzeugung paralleler strahlen und ebenen
US20050057736A1 (en) Method and apparatus for multiphoton-absorption exposure wherein exposure condition is changed with depth of convergence position
EP0262855A3 (de) Erzeugung von gepulsten Elektronenstrahlen
ATE342499T1 (de) Vorrichtung und verfahren zur automatischen erzeugung einer wärmevermeidungsreaktion von nematoden
ATE289691T1 (de) Vorrichtung und verfahren zum lesen eines optischen codes
EP0254864A3 (en) Method for determining intracellular mineral levels
ATE467901T1 (de) Optisches beleuchtungssystem, belichtungssystem und belichtungsverfahren
JPH0376569A (ja) 細胞プロセシング装置および方法
JPS6213117B2 (de)
JPH03172167A (ja) 細胞プロセシング装置および方法
EP1149280B1 (de) Verfahren und vorrichtung für automatisierte chromophor-unterstützte laser/licht-inaktivierung (cali)
GB2182455A (en) Optical switch or information store

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings
8310 Action for declaration of annulment
8313 Request for invalidation rejected/withdrawn