CA2783351C - Tal effector-mediated dna modification - Google Patents

Tal effector-mediated dna modification Download PDF

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Publication number
CA2783351C
CA2783351C CA2783351A CA2783351A CA2783351C CA 2783351 C CA2783351 C CA 2783351C CA 2783351 A CA2783351 A CA 2783351A CA 2783351 A CA2783351 A CA 2783351A CA 2783351 C CA2783351 C CA 2783351C
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Prior art keywords
recognizing
sequence
tal effector
cell
endonuclease
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CA2783351A
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English (en)
French (fr)
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CA2783351A1 (en
Inventor
Daniel F. Voytas
Adam Bogdanove
Feng Zhang
Michelle Christian
Tomas Cermak
Clarice Lauer Schmidt
Erin Doyle
Li Wang
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Iowa State University Research Foundation Inc
University of Minnesota System
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Iowa State University Research Foundation Inc
University of Minnesota System
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Application filed by Iowa State University Research Foundation Inc, University of Minnesota System filed Critical Iowa State University Research Foundation Inc
Publication of CA2783351A1 publication Critical patent/CA2783351A1/en
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
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    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)

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CA2783351A 2009-12-10 2010-12-10 Tal effector-mediated dna modification Active CA2783351C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US28532409P 2009-12-10 2009-12-10
US61/285,324 2009-12-10
US35210810P 2010-06-07 2010-06-07
US61/352,108 2010-06-07
US36668510P 2010-07-22 2010-07-22
US61/366,685 2010-07-22
PCT/US2010/059932 WO2011072246A2 (en) 2009-12-10 2010-12-10 Tal effector-mediated dna modification

Publications (2)

Publication Number Publication Date
CA2783351A1 CA2783351A1 (en) 2011-06-16
CA2783351C true CA2783351C (en) 2021-09-07

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CA2783351A Active CA2783351C (en) 2009-12-10 2010-12-10 Tal effector-mediated dna modification

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Country Link
US (10) US8586363B2 (cg-RX-API-DMAC10.html)
EP (3) EP2816112B1 (cg-RX-API-DMAC10.html)
JP (3) JP2013513389A (cg-RX-API-DMAC10.html)
KR (2) KR102110725B1 (cg-RX-API-DMAC10.html)
CN (2) CN106834320B (cg-RX-API-DMAC10.html)
AU (2) AU2010327998B2 (cg-RX-API-DMAC10.html)
CA (1) CA2783351C (cg-RX-API-DMAC10.html)
DK (2) DK2510096T4 (cg-RX-API-DMAC10.html)
ES (2) ES2527997T5 (cg-RX-API-DMAC10.html)
HU (1) HUE041436T2 (cg-RX-API-DMAC10.html)
IL (2) IL220234B (cg-RX-API-DMAC10.html)
NO (1) NO2510096T3 (cg-RX-API-DMAC10.html)
PL (2) PL2816112T3 (cg-RX-API-DMAC10.html)
PT (2) PT2816112T (cg-RX-API-DMAC10.html)
SG (1) SG181601A1 (cg-RX-API-DMAC10.html)
TR (1) TR201815882T4 (cg-RX-API-DMAC10.html)
WO (1) WO2011072246A2 (cg-RX-API-DMAC10.html)

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